AlfalfaGEDB | Msa0810550

M. sativa cultivar zhongmu-4 / Msa0810550

KEGG: K18485
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810550	sp\|Q9C7T4\|BH096_ARATH	46.939	343	131	11	1	319	1	316	1.91e-83	258

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810550	G7KAJ7	79.938	324	41	2	1	324	1	300	0.0	525

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TFs/TRs:

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Gene ID	Type	Classification
Msa0810550	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0732160	Msa0810550	0.811837	5.731740e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810550	MtrunA17_Chr5g0426851	79.938	324	41	2	1	324	1	300	0.0	525
Msa0810550	MtrunA17_Chr3g0132471	53.687	339	117	13	1	321	1	317	1.95e-101	301
Msa0810550	MtrunA17_Chr7g0273081	43.902	328	147	12	1	321	14	311	1.98e-74	231
Msa0810550	MtrunA17_Chr8g0366551	53.271	214	91	5	113	324	114	320	6.50e-67	213
Msa0810550	MtrunA17_Chr1g0191571	40.176	341	161	9	1	319	14	333	3.41e-66	211
Msa0810550	MtrunA17_Chr8g0345871	44.660	206	91	3	112	312	170	357	8.61e-47	162
Msa0810550	MtrunA17_Chr0c01g0489461	41.667	216	116	5	104	312	176	388	1.89e-44	156
Msa0810550	MtrunA17_Chr2g0287761	38.068	176	105	2	123	298	1	172	7.50e-34	123
Msa0810550	MtrunA17_Chr1g0158941	30.068	296	150	10	46	317	27	289	7.71e-26	104
Msa0810550	MtrunA17_Chr3g0126361	37.719	114	66	2	46	154	19	132	4.18e-13	65.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810550	AT1G72210.1	46.939	343	131	11	1	319	1	316	1.94e-84	258
Msa0810550	AT1G72210.2	45.253	316	123	10	1	292	1	290	1.12e-72	227
Msa0810550	AT1G22490.1	43.976	332	144	12	1	319	1	303	6.83e-70	220
Msa0810550	AT1G22490.2	41.967	305	136	11	1	292	1	277	4.89e-57	187
Msa0810550	AT4G01460.1	39.437	213	112	3	113	319	105	306	1.08e-42	150
Msa0810550	AT4G01460.2	39.437	213	112	3	113	319	105	306	1.08e-42	150
Msa0810550	AT5G46690.1	44.118	238	89	8	113	319	78	302	6.27e-42	148
Msa0810550	AT5G65320.1	40.097	207	109	2	119	319	98	295	1.55e-41	146
Msa0810550	AT2G46810.2	39.614	207	95	5	114	312	112	296	2.58e-40	143
Msa0810550	AT3G24140.2	39.367	221	118	3	103	312	177	392	3.68e-40	145

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Find 65 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGGAAGGAGGAAGAG+TGG	0.533377	5_3:+39086071	None:intergenic
AAGAAGAATCCCAATTTGAA+AGG	0.545406	5_3:+39086145	None:intergenic
AAGGACATTATCATCCACAC+TGG	0.650900	5_3:+39085179	None:intergenic
AATTAATAATAGGTAGAAGA+AGG	0.501607	5_3:-39084390	Msa0810550:intron
ACAAGGACAGGTTATGAAGA+TGG	0.569307	5_3:-39085250	Msa0810550:CDS
ACACCTTAATGTTTCCAGTG+TGG	0.628319	5_3:-39085193	Msa0810550:CDS
ACATAATCTACAACTCATGA+AGG	0.534004	5_3:-39085496	Msa0810550:CDS
ACCAACCATAATCAGTCACG+TGG	0.705729	5_3:-39085339	Msa0810550:CDS
ACCCTTTGTACATAAGTAGG+AGG	0.570239	5_3:+39085883	None:intergenic
ACTTGGAGTGGTTTCAACTA+AGG	0.406246	5_3:+39086257	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTGTAAATCAATGGATATTG+TGG	+	chr5_3:39084326-39084345	None:intergenic	30.0%
TCTTTGCAACTGTAAATCAA+TGG	+	chr5_3:39084335-39084354	None:intergenic	30.0%
AAGAAGAATCCCAATTTGAA+AGG	+	chr5_3:39084481-39084500	None:intergenic	30.0%
CTTCAAATGTTAAAGACCAA+TGG	-	chr5_3:39084504-39084523	Msa0810550:intron	30.0%
ATTACCCTTTGTACATAAGT+AGG	+	chr5_3:39084746-39084765	None:intergenic	30.0%
CTACATGTGGTTTAAAAAAC+AGG	-	chr5_3:39085052-39085071	Msa0810550:intron	30.0%
ACATAATCTACAACTCATGA+AGG	-	chr5_3:39085127-39085146	Msa0810550:intron	30.0%
CATAATCTACAACTCATGAA+GGG	-	chr5_3:39085128-39085147	Msa0810550:intron	30.0%
ATGAAAAACTCAGTAAAAGG+TGG	+	chr5_3:39085215-39085234	None:intergenic	30.0%
ATGTTTCCTCAATACTCTAT+GGG	-	chr5_3:39085233-39085252	Msa0810550:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	39084299	39086346	39084299	ID=Msa0810550;Name=Msa0810550
chr5_3	mRNA	39084299	39086346	39084299	ID=Msa0810550-mRNA-1;Parent=Msa0810550;Name=Msa0810550-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|324
chr5_3	exon	39085885	39086346	39085885	ID=Msa0810550-mRNA-1:exon:11866;Parent=Msa0810550-mRNA-1
chr5_3	exon	39085161	39085571	39085161	ID=Msa0810550-mRNA-1:exon:11865;Parent=Msa0810550-mRNA-1
chr5_3	exon	39084299	39084400	39084299	ID=Msa0810550-mRNA-1:exon:11864;Parent=Msa0810550-mRNA-1
chr5_3	CDS	39085885	39086346	39085885	ID=Msa0810550-mRNA-1:cds;Parent=Msa0810550-mRNA-1
chr5_3	CDS	39085161	39085571	39085161	ID=Msa0810550-mRNA-1:cds;Parent=Msa0810550-mRNA-1
chr5_3	CDS	39084299	39084400	39084299	ID=Msa0810550-mRNA-1:cds;Parent=Msa0810550-mRNA-1

Gene Sequence

>Msa0810550

ATGGCCTTAGAGGCTATAGTTTTTCCACAATATCCATTGATTTACAGTTGCAAAGACTACTTGTACTCCTTAGTTGAAACCACTCCAAGTGAAGGATCTTGGAGCCACAACTACGGTTGCCAAACAAAAGATGAAGAAGATCTTTTTAAAATCATCAGTAATAGCTTAGATCACAATATCCTTTCAAATTGGGATTCTTCTTCTCCTTCAAATGTTAAAGACCAATGGAATTCACACTCTTCACCTGAAACTTCCACTCTTCCTCCTTCCACTTTTGCATCCACCACAACAACCATAGCCCCTCCTCGGAGAAAACGACGACGCGCTGTGAATGCAAAAAACGAGGAGGAAATCGAGAGCCAGAGGATGACTCACATTGCAGTTGAGCGCAATCGAAGAAAACAGATGAATGAGTACCTCGATGTCCTCAAATCCTTGATGCCTCCTACTTATGTACAAAGGGGTGATCAAGCGTCAATAGTTGGCGGTGCAATTAACTTCTTGAAGGAGCTAGAACATAATCTACAACTCATGAAGGGACAAAAGAAAATAAACAAAGAAGCTCATGAAAATAGTTTCATTACTTGTTCTTGTTCATCACCACCTTTTACTGAGTTTTTCATGTTTCCTCAATACTCTATGGGTGCACCTCAGAACATCACGTGCTACCCTACCAACCATAATCAGTCACGTGGCATGGCAGATATAGAAGTGACTTTGGTTGATAGTCATACAAATATAAATATAATGTTGAAAAAACGACAAGGACAGGTTATGAAGATGGTTGTTGGGATTCAAAATCTTGGCTTCAACATTCTACACCTTAATGTTTCCAGTGTGGATGATAATGTCCTTGTCTCGGTTAGTGCTAAGGTAGAAGAAGGGTGTCAACTACTGAACACAGTGGATGGAATTGCAGCTGCAGTGAATGAGCTATCACGTAGAATTCAGGTGGAAGATGCTGCTTTGACCTGA

Protein sequence

>Msa0810550

MALEAIVFPQYPLIYSCKDYLYSLVETTPSEGSWSHNYGCQTKDEEDLFKIISNSLDHNILSNWDSSSPSNVKDQWNSHSSPETSTLPPSTFASTTTTIAPPRRKRRRAVNAKNEEEIESQRMTHIAVERNRRKQMNEYLDVLKSLMPPTYVQRGDQASIVGGAINFLKELEHNLQLMKGQKKINKEAHENSFITCSCSSPPFTEFFMFPQYSMGAPQNITCYPTNHNQSRGMADIEVTLVDSHTNINIMLKKRQGQVMKMVVGIQNLGFNILHLNVSSVDDNVLVSVSAKVEEGCQLLNTVDGIAAAVNELSRRIQVEDAALT*