AlfalfaGEDB | Msa0732160

M. sativa cultivar zhongmu-4 / Msa0732160

KEGG: K18485
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0732160	XP_003615193.2	74.691	324	42	5	1	308	1	300	1.30e-165	472

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0732160	sp\|Q9C7T4\|BH096_ARATH	47.761	335	124	12	1	303	1	316	5.72e-82	253

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0732160	G7KAJ7	74.691	324	42	5	1	308	1	300	6.23e-166	472

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TFs/TRs:

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Gene ID	Type	Classification
Msa0732160	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0732160	Msa0773240	0.869768	2.416890e-66	-8.615850e-47
Msa0732160	Msa0810550	0.811837	5.731740e-51	-8.615850e-47
Msa0732160	Msa0855790	0.852100	5.758913e-61	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0732160	MtrunA17_Chr5g0426851	74.691	324	42	5	1	308	1	300	1.20e-169	472
Msa0732160	MtrunA17_Chr3g0132471	52.568	331	117	11	1	305	1	317	4.12e-96	286
Msa0732160	MtrunA17_Chr7g0273081	44.025	318	145	12	1	305	14	311	1.65e-71	223
Msa0732160	MtrunA17_Chr8g0366551	52.113	213	94	5	98	308	114	320	1.01e-63	204
Msa0732160	MtrunA17_Chr1g0191571	42.378	328	156	10	1	303	14	333	4.40e-63	202
Msa0732160	MtrunA17_Chr8g0345871	43.689	206	92	4	97	296	170	357	8.53e-44	153
Msa0732160	MtrunA17_Chr0c01g0489461	40.278	216	116	6	90	296	177	388	3.30e-41	147
Msa0732160	MtrunA17_Chr2g0287761	38.636	176	103	3	108	282	1	172	1.23e-32	119
Msa0732160	MtrunA17_Chr1g0158941	30.072	276	160	6	46	301	27	289	4.95e-27	107

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0732160	AT1G72210.1	47.761	335	124	12	1	303	1	316	5.82e-83	253
Msa0732160	AT1G72210.2	46.104	308	116	11	1	276	1	290	6.57e-71	222
Msa0732160	AT1G22490.1	44.969	318	145	11	1	303	1	303	3.16e-67	213
Msa0732160	AT1G22490.2	43.003	293	134	9	1	276	1	277	1.47e-54	181
Msa0732160	AT5G46690.1	44.958	238	86	9	98	303	78	302	9.90e-43	150
Msa0732160	AT5G65320.1	39.614	207	109	3	104	303	98	295	5.06e-40	142
Msa0732160	AT4G01460.1	38.967	213	112	4	98	303	105	306	6.07e-40	142
Msa0732160	AT4G01460.2	38.967	213	112	4	98	303	105	306	6.07e-40	142
Msa0732160	AT2G46810.2	39.409	203	100	7	99	296	112	296	2.69e-37	135
Msa0732160	AT3G24140.2	38.462	221	119	4	88	296	177	392	3.09e-37	137

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Find 63 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGGAAGTGGGAAGAG+TGG	0.541468	5_1:+44221264	None:intergenic
AAGGACATTATCATCCACAC+TGG	0.650900	5_1:+44220376	None:intergenic
AAGGAGGCATCAAGGATTTG+AGG	0.530586	5_1:+44221092	None:intergenic
AATTAATAATAGGTAGAAGA+AGG	0.501607	5_1:-44219589	Msa0732160:intron
ACAAGGACAGGTTATGAAGA+TGG	0.569307	5_1:-44220447	Msa0732160:CDS
ACACCTTAATGTTTCCAGTG+TGG	0.628319	5_1:-44220390	Msa0732160:CDS
ACATAATCTACAATTCATGA+AGG	0.570662	5_1:-44220693	Msa0732160:CDS
ACCAACCATAATCAGTCACG+TGG	0.705729	5_1:-44220536	Msa0732160:CDS
ACGAGGGGCTGTGGTTGTTG+TGG	0.561823	5_1:+44221234	None:intergenic
ACTTGGAGTGGTTTCAACTA+AGG	0.406246	5_1:+44221405	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTGTAAATCAATGGATATTG+TGG	+	chr5_1:44219525-44219544	None:intergenic	30.0%
TCTTTGCAACTGTAAATCAA+TGG	+	chr5_1:44219534-44219553	None:intergenic	30.0%
TGAACCACATGTAGAATTTA+GGG	+	chr5_1:44220197-44220216	None:intergenic	30.0%
TTGAACCACATGTAGAATTT+AGG	+	chr5_1:44220198-44220217	None:intergenic	30.0%
ATGAAAAACTCAGTAAAAGG+TGG	+	chr5_1:44220365-44220384	None:intergenic	30.0%
ATGTTTCCTCAATACTCTAT+GGG	-	chr5_1:44220383-44220402	Msa0732160:CDS	30.0%
CTCGTTAAATAAATGATGAG+TGG	-	chr5_1:44220742-44220761	Msa0732160:CDS	30.0%
TAGAGAAATATTCGATTGAG+AGG	-	chr5_1:44220784-44220803	Msa0732160:intron	30.0%
AGAGAAATATTCGATTGAGA+GGG	-	chr5_1:44220785-44220804	Msa0732160:intron	30.0%
CTGTAAATCAATGGATATTG+TGG	+	chr5_1:44219525-44219544	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	44219498	44221494	44219498	ID=Msa0732160;Name=Msa0732160
chr5_1	mRNA	44219498	44221494	44219498	ID=Msa0732160-mRNA-1;Parent=Msa0732160;Name=Msa0732160-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|308
chr5_1	exon	44221081	44221494	44221081	ID=Msa0732160-mRNA-1:exon:12578;Parent=Msa0732160-mRNA-1
chr5_1	exon	44220358	44220768	44220358	ID=Msa0732160-mRNA-1:exon:12577;Parent=Msa0732160-mRNA-1
chr5_1	exon	44219498	44219599	44219498	ID=Msa0732160-mRNA-1:exon:12576;Parent=Msa0732160-mRNA-1
chr5_1	CDS	44221081	44221494	44221081	ID=Msa0732160-mRNA-1:cds;Parent=Msa0732160-mRNA-1
chr5_1	CDS	44220358	44220768	44220358	ID=Msa0732160-mRNA-1:cds;Parent=Msa0732160-mRNA-1
chr5_1	CDS	44219498	44219599	44219498	ID=Msa0732160-mRNA-1:cds;Parent=Msa0732160-mRNA-1

Gene Sequence

>Msa0732160

ATGGCCTTAGAGGCTATAGTTTTTCCACAATATCCATTGATTTACAGTTGCAAAGACTACTTGTACTCCTTAGTTGAAACCACTCCAAGTGAAGGATCTTGGAGCCACAACTACGGTTGCCAAACAAAAGACGAAGAAGATCTTTTTAAAATCAGCAATAACTTCGATCATAATCTCCTTTCAAATTGGGATTCTTCCCCAGAAACTACCACTCTTCCCACTTCCACTTTTGCATCCACCACAACAACCACAGCCCCTCGTCAGAGCAAACGACGACGCACTGTGAATGCAAAAAATGAGGAGGAAATCGAGAGCCAGAGGATGACTCACATTGCAGTTGAGCGCAATCGAAGAAAACAGATGAATGAGTACCTCGATGTCCTCAAATCCTTGATGCCTCCTTATGCAAAAAGGGGTGATCAAGCATCAATAGTTGGCGGTGCAATTAACTTCTTGAAGGAGCTAGAACATAATCTACAATTCATGAAGGGACAAAAGAAAATAAACAAACAAGCTCATGAAAATAGTTTCATTACTTGTTCTTGTTCATCACCACCTTTTACTGAGTTTTTCATGTTTCCTCAATACTCTATGGGTGCACCTCAGAACATCACGTGCTACCCTACCAACCATAATCAGTCACGTGGCATGGCAGATATAGAAGTGACTTTGGTTGATAGTCATACAAATATAAATATAATGTTGAAAAAACGACAAGGACAGGTTATGAAGATGGTTGTTGGGATTCAAAATCTTGGCTTCAACATTCTACACCTTAATGTTTCCAGTGTGGATGATAATGTCCTTGTCTCGGTTAGTGCTAAGGTAGAAGAAGGGTGTCAACTACTGAACACAGTGGATGGAATTGCAGCTGCAGTGAATGAGCTATCACGTAGAATTCAGGTGGAAGATGCTGCTTTGACCTGA

Protein sequence

>Msa0732160

MALEAIVFPQYPLIYSCKDYLYSLVETTPSEGSWSHNYGCQTKDEEDLFKISNNFDHNLLSNWDSSPETTTLPTSTFASTTTTTAPRQSKRRRTVNAKNEEEIESQRMTHIAVERNRRKQMNEYLDVLKSLMPPYAKRGDQASIVGGAINFLKELEHNLQFMKGQKKINKQAHENSFITCSCSSPPFTEFFMFPQYSMGAPQNITCYPTNHNQSRGMADIEVTLVDSHTNINIMLKKRQGQVMKMVVGIQNLGFNILHLNVSSVDDNVLVSVSAKVEEGCQLLNTVDGIAAAVNELSRRIQVEDAALT*