AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0812160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0812160 MtrunA17_Chr4g0043221 100.000 98 0 0 1 98 1 98 2.34e-66 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0812160 AT1G76860.1 89.796 98 9 1 1 98 1 97 9.87e-59 175
Msa0812160 AT1G21190.1 86.458 96 13 0 3 98 2 97 8.05e-58 173

Find 47 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCAACTCAAAGCTCTTTC+TGG 0.156432 5_3:+42083429 Msa0812160:three_prime_UTR
ATCATTGGCGTTTAAGCTTA+TGG 0.251620 5_3:-42083263 None:intergenic
CAACATCTTAATATGATTCT+TGG 0.270259 5_3:+42082394 Msa0812160:CDS
CGCCATCTCCCCTAACAAAA+AGG 0.290294 5_3:-42082965 None:intergenic
GACTTCCTGAATCCTAAGTT+TGG 0.349750 5_3:+42083397 Msa0812160:three_prime_UTR
CTAAACCAAACTTAGGATTC+AGG 0.374091 5_3:-42083402 None:intergenic
ATGAACAAGGGTTATACTTA+TGG 0.392555 5_3:+42083208 Msa0812160:three_prime_UTR
TAGGGGAGATGGCGTGATAT+TGG 0.403792 5_3:+42082974 Msa0812160:CDS
TTCATTGTGAGTAGTGAATC+TGG 0.406569 5_3:+42082160 Msa0812160:five_prime_UTR
AGACATATGAAGAAATTGTC+AGG 0.427463 5_3:+42082458 Msa0812160:CDS
TATAGCAAATCAAGAATCAT+TGG 0.435889 5_3:-42083278 None:intergenic
AAGCGCAGTGAAGGAGCCTT+TGG 0.436284 5_3:+42082219 Msa0812160:CDS
CTTCTAAGTTCTAGTTACAT+TGG 0.450409 5_3:+42083325 Msa0812160:three_prime_UTR
GGAAAAGCTAAACCAAACTT+AGG 0.450798 5_3:-42083409 None:intergenic
TACATTGGAGATCTTGAACC+TGG 0.462550 5_3:+42083340 Msa0812160:three_prime_UTR
GACCAGAAAGAGCTTTGAGT+TGG 0.474701 5_3:-42083431 None:intergenic
AGGAAGTCTAGAAACTTTGA+AGG 0.480064 5_3:-42083382 None:intergenic
GAGGATAATCTAATATCATA+AGG 0.485064 5_3:-42083105 None:intergenic
ACCAGAAAGAGCTTTGAGTT+GGG 0.492462 5_3:-42083430 None:intergenic
AGAGGAAGAAAGCGCAGTGA+AGG 0.494057 5_3:+42082210 Msa0812160:CDS
ACATTGGAGATCTTGAACCT+GGG 0.500499 5_3:+42083341 Msa0812160:three_prime_UTR
AGGAGCCTTTGGATCTCATA+AGG 0.521514 5_3:+42082230 Msa0812160:CDS
AAGAAGAATGGCGGGGACAG+AGG 0.529954 5_3:+42082192 Msa0812160:exon
TCATGGAATGCTCATGAACA+AGG 0.539148 5_3:+42083195 Msa0812160:three_prime_UTR
AATTGAGAAAGAAGAATGGC+GGG 0.544172 5_3:+42082184 Msa0812160:exon
CGTTCTGATAGAGAGCTTCG+TGG 0.548358 5_3:+42082286 Msa0812160:CDS
CTCAGCCTTATGAGATCCAA+AGG 0.555367 5_3:-42082235 None:intergenic
GTACCTCCTTGTTGAAGCGA+TGG 0.558308 5_3:-42081769 None:intergenic
GAGCTGTTGTTACATAGATG+TGG 0.561086 5_3:+42083238 Msa0812160:three_prime_UTR
CCACTGAGGACTGCATAACA+TGG 0.569381 5_3:+42083005 Msa0812160:exon
GGGTTATACTTATGGCAGCT+TGG 0.573830 5_3:+42083216 Msa0812160:three_prime_UTR
ATCTCTCCATCGCTTCAACA+AGG 0.583163 5_3:+42081763 Msa0812160:five_prime_UTR
TGGTAATTGAGAAAGAAGAA+TGG 0.601575 5_3:+42082180 Msa0812160:exon
CATTGGAGATCTTGAACCTG+GGG 0.601867 5_3:+42083342 Msa0812160:three_prime_UTR
ACTAGCAAACATGCATGTTG+AGG 0.616942 5_3:-42083124 None:intergenic
CACGAAGCTCTCTATCAGAA+CGG 0.619250 5_3:-42082285 None:intergenic
CCATGTTATGCAGTCCTCAG+TGG 0.619717 5_3:-42083005 None:intergenic
GACATATGAAGAAATTGTCA+GGG 0.620564 5_3:+42082459 Msa0812160:CDS
TGTTATGCAGTCCTCAGTGG+TGG 0.628383 5_3:-42083002 None:intergenic
TAATTGAGAAAGAAGAATGG+CGG 0.660632 5_3:+42082183 Msa0812160:exon
CATGGAATGCTCATGAACAA+GGG 0.663566 5_3:+42083196 Msa0812160:three_prime_UTR
ATACAACATTCAAACACCCC+AGG 0.667078 5_3:-42083358 None:intergenic
TCTAATTGCAGACTACAAAG+CGG 0.689189 5_3:+42082931 Msa0812160:intron
ATCTAATATCATAAGGAACG+AGG 0.696193 5_3:-42083098 None:intergenic
ATTGAGAAAGAAGAATGGCG+GGG 0.698364 5_3:+42082185 Msa0812160:exon
TCTCCATCGCTTCAACAAGG+AGG 0.716351 5_3:+42081766 Msa0812160:five_prime_UTR
TATTGGTTTCTCCACCACTG+AGG 0.734600 5_3:+42082991 Msa0812160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTATTTATTTTTTA+GGG + chr5_3:42082136-42082155 Msa0812160:intron 0.0%
!!! TTTATTTTATTTATTTTTTA+GGG + chr5_3:42082136-42082155 Msa0812160:intron 0.0%
!!! TTTTTTTGTATTTGAGATTT+AGG + chr5_3:42081892-42081911 Msa0812160:intron 15.0%
!!! TTTTTTGTATTTGAGATTTA+GGG + chr5_3:42081893-42081912 Msa0812160:intron 15.0%
!! TAAAAGATTATTGTAGTTTA+AGG + chr5_3:42082593-42082612 Msa0812160:intron 15.0%
!!! TATTTTTTAGATTAGATTAC+TGG + chr5_3:42082715-42082734 Msa0812160:intron 15.0%
!!! TTTTTTTGTATTTGAGATTT+AGG + chr5_3:42081892-42081911 Msa0812160:intron 15.0%
!!! TTTTTTGTATTTGAGATTTA+GGG + chr5_3:42081893-42081912 Msa0812160:intron 15.0%
!! TAAAAGATTATTGTAGTTTA+AGG + chr5_3:42082593-42082612 Msa0812160:intron 15.0%
!!! TATTTTTTAGATTAGATTAC+TGG + chr5_3:42082715-42082734 Msa0812160:intron 15.0%
!!! TTTTTTCTTGTGATTTTGAA+TGG + chr5_3:42081847-42081866 Msa0812160:intron 20.0%
!!! TGATTTTGAATGGTTATTTA+GGG + chr5_3:42081857-42081876 Msa0812160:intron 20.0%
!! CAAATACAAAAAAACTAACA+AGG - chr5_3:42081886-42081905 None:intergenic 20.0%
!! AAAAACAATTCATTAAACCT+GGG - chr5_3:42082056-42082075 None:intergenic 20.0%
!! AAAAAACAATTCATTAAACC+TGG - chr5_3:42082057-42082076 None:intergenic 20.0%
!!! TTAATTAATAAGCATTGTTG+AGG + chr5_3:42082531-42082550 Msa0812160:intron 20.0%
!!! TAATTAATAAGCATTGTTGA+GGG + chr5_3:42082532-42082551 Msa0812160:intron 20.0%
!!! AAGTTATAGAATTTTTGTGA+TGG - chr5_3:42083066-42083085 None:intergenic 20.0%
!!! TTTTTTCTTGTGATTTTGAA+TGG + chr5_3:42081847-42081866 Msa0812160:intron 20.0%
!!! TGATTTTGAATGGTTATTTA+GGG + chr5_3:42081857-42081876 Msa0812160:intron 20.0%
!! CAAATACAAAAAAACTAACA+AGG - chr5_3:42081886-42081905 None:intergenic 20.0%
!! AAAAACAATTCATTAAACCT+GGG - chr5_3:42082056-42082075 None:intergenic 20.0%
!! AAAAAACAATTCATTAAACC+TGG - chr5_3:42082057-42082076 None:intergenic 20.0%
!!! TTAATTAATAAGCATTGTTG+AGG + chr5_3:42082531-42082550 Msa0812160:intron 20.0%
!!! TAATTAATAAGCATTGTTGA+GGG + chr5_3:42082532-42082551 Msa0812160:intron 20.0%
!!! AAGTTATAGAATTTTTGTGA+TGG - chr5_3:42083066-42083085 None:intergenic 20.0%
!! GTGATTTTGAATGGTTATTT+AGG + chr5_3:42081856-42081875 Msa0812160:intron 25.0%
!!! GTGTGTTAATTAATTTGTGA+AGG + chr5_3:42082113-42082132 Msa0812160:intron 25.0%
! TGAAAGAAAAAAGAGATGAA+AGG - chr5_3:42082338-42082357 None:intergenic 25.0%
! TGATTTCAATTTCAATTTCC+AGG + chr5_3:42082363-42082382 Msa0812160:intron 25.0%
! CAACATCTTAATATGATTCT+TGG + chr5_3:42082394-42082413 Msa0812160:CDS 25.0%
!! TACTTTCTACAATTTAGCTT+TGG - chr5_3:42082501-42082520 None:intergenic 25.0%
!! ATCTTTTATCAAACTGAAGA+AGG - chr5_3:42082581-42082600 None:intergenic 25.0%
!!! TTGTAGTTTAAGGTGTTTTA+AGG + chr5_3:42082603-42082622 Msa0812160:intron 25.0%
! ATCACAGTTGTGAAAAAATA+AGG - chr5_3:42082761-42082780 None:intergenic 25.0%
! AGAAAAACAAAAATCATCCA+AGG - chr5_3:42082911-42082930 None:intergenic 25.0%
! GAGGATAATCTAATATCATA+AGG - chr5_3:42083108-42083127 None:intergenic 25.0%
! TATAGCAAATCAAGAATCAT+TGG - chr5_3:42083281-42083300 None:intergenic 25.0%
!! GTGATTTTGAATGGTTATTT+AGG + chr5_3:42081856-42081875 Msa0812160:intron 25.0%
!!! GTGTGTTAATTAATTTGTGA+AGG + chr5_3:42082113-42082132 Msa0812160:intron 25.0%
! TGAAAGAAAAAAGAGATGAA+AGG - chr5_3:42082338-42082357 None:intergenic 25.0%
! TGATTTCAATTTCAATTTCC+AGG + chr5_3:42082363-42082382 Msa0812160:intron 25.0%
! CAACATCTTAATATGATTCT+TGG + chr5_3:42082394-42082413 Msa0812160:CDS 25.0%
!! TACTTTCTACAATTTAGCTT+TGG - chr5_3:42082501-42082520 None:intergenic 25.0%
!! ATCTTTTATCAAACTGAAGA+AGG - chr5_3:42082581-42082600 None:intergenic 25.0%
!!! TTGTAGTTTAAGGTGTTTTA+AGG + chr5_3:42082603-42082622 Msa0812160:intron 25.0%
! ATCACAGTTGTGAAAAAATA+AGG - chr5_3:42082761-42082780 None:intergenic 25.0%
! AGAAAAACAAAAATCATCCA+AGG - chr5_3:42082911-42082930 None:intergenic 25.0%
! GAGGATAATCTAATATCATA+AGG - chr5_3:42083108-42083127 None:intergenic 25.0%
! TATAGCAAATCAAGAATCAT+TGG - chr5_3:42083281-42083300 None:intergenic 25.0%
TGGTAATTGAGAAAGAAGAA+TGG + chr5_3:42082180-42082199 Msa0812160:exon 30.0%
TAATTGAGAAAGAAGAATGG+CGG + chr5_3:42082183-42082202 Msa0812160:exon 30.0%
AGACATATGAAGAAATTGTC+AGG + chr5_3:42082458-42082477 Msa0812160:CDS 30.0%
GACATATGAAGAAATTGTCA+GGG + chr5_3:42082459-42082478 Msa0812160:CDS 30.0%
ACTCTGATTTAAGAACTATG+AGG + chr5_3:42082637-42082656 Msa0812160:intron 30.0%
AGGTCAATAGTTGAAAGAAA+AGG - chr5_3:42082741-42082760 None:intergenic 30.0%
CCAATAAAACGATAGAAACT+TGG - chr5_3:42082817-42082836 None:intergenic 30.0%
! CCAAGTTTCTATCGTTTTAT+TGG + chr5_3:42082814-42082833 Msa0812160:intron 30.0%
! ATCATCCAAGGAAATTTTGA+TGG - chr5_3:42082899-42082918 None:intergenic 30.0%
ATCTAATATCATAAGGAACG+AGG - chr5_3:42083101-42083120 None:intergenic 30.0%
!! TCTTTTTAGCATGAGAATCA+TGG + chr5_3:42083178-42083197 Msa0812160:three_prime_UTR 30.0%
ATGAACAAGGGTTATACTTA+TGG + chr5_3:42083208-42083227 Msa0812160:three_prime_UTR 30.0%
! CTTCTAAGTTCTAGTTACAT+TGG + chr5_3:42083325-42083344 Msa0812160:three_prime_UTR 30.0%
TGGTAATTGAGAAAGAAGAA+TGG + chr5_3:42082180-42082199 Msa0812160:exon 30.0%
TAATTGAGAAAGAAGAATGG+CGG + chr5_3:42082183-42082202 Msa0812160:exon 30.0%
AGACATATGAAGAAATTGTC+AGG + chr5_3:42082458-42082477 Msa0812160:CDS 30.0%
GACATATGAAGAAATTGTCA+GGG + chr5_3:42082459-42082478 Msa0812160:CDS 30.0%
ACTCTGATTTAAGAACTATG+AGG + chr5_3:42082637-42082656 Msa0812160:intron 30.0%
AGGTCAATAGTTGAAAGAAA+AGG - chr5_3:42082741-42082760 None:intergenic 30.0%
CCAATAAAACGATAGAAACT+TGG - chr5_3:42082817-42082836 None:intergenic 30.0%
! CCAAGTTTCTATCGTTTTAT+TGG + chr5_3:42082814-42082833 Msa0812160:intron 30.0%
! ATCATCCAAGGAAATTTTGA+TGG - chr5_3:42082899-42082918 None:intergenic 30.0%
ATCTAATATCATAAGGAACG+AGG - chr5_3:42083101-42083120 None:intergenic 30.0%
!! TCTTTTTAGCATGAGAATCA+TGG + chr5_3:42083178-42083197 Msa0812160:three_prime_UTR 30.0%
ATGAACAAGGGTTATACTTA+TGG + chr5_3:42083208-42083227 Msa0812160:three_prime_UTR 30.0%
! CTTCTAAGTTCTAGTTACAT+TGG + chr5_3:42083325-42083344 Msa0812160:three_prime_UTR 30.0%
AGATTGAAAGAGAAAGATCG+AGG - chr5_3:42081798-42081817 None:intergenic 35.0%
ATTCGATTGTGAAACTAACC+TGG + chr5_3:42082017-42082036 Msa0812160:intron 35.0%
TTCATTGTGAGTAGTGAATC+TGG + chr5_3:42082160-42082179 Msa0812160:five_prime_UTR 35.0%
AATTGAGAAAGAAGAATGGC+GGG + chr5_3:42082184-42082203 Msa0812160:exon 35.0%
TAAGATGTTGATCATAAGCC+TGG - chr5_3:42082384-42082403 None:intergenic 35.0%
!!! AGGTGTTTTAAGGTTTTGAC+TGG + chr5_3:42082613-42082632 Msa0812160:intron 35.0%
!!! GGTGTTTTAAGGTTTTGACT+GGG + chr5_3:42082614-42082633 Msa0812160:intron 35.0%
! ATATCTACTAACTTGTGACC+TGG + chr5_3:42082779-42082798 Msa0812160:intron 35.0%
ACTTGGTCAAACAAATAACC+AGG - chr5_3:42082800-42082819 None:intergenic 35.0%
!! TCCAAGGAAATTTTGATGGT+TGG - chr5_3:42082895-42082914 None:intergenic 35.0%
TCTAATTGCAGACTACAAAG+CGG + chr5_3:42082931-42082950 Msa0812160:intron 35.0%
! AGTTCCGTTCCTTTTTGTTA+GGG + chr5_3:42082956-42082975 Msa0812160:CDS 35.0%
TCTCATGCTAAAAAGAGAAC+AGG - chr5_3:42083174-42083193 None:intergenic 35.0%
ATCATTGGCGTTTAAGCTTA+TGG - chr5_3:42083266-42083285 None:intergenic 35.0%
AGGAAGTCTAGAAACTTTGA+AGG - chr5_3:42083385-42083404 None:intergenic 35.0%
CTAAACCAAACTTAGGATTC+AGG - chr5_3:42083405-42083424 None:intergenic 35.0%
GGAAAAGCTAAACCAAACTT+AGG - chr5_3:42083412-42083431 None:intergenic 35.0%
AGATTGAAAGAGAAAGATCG+AGG - chr5_3:42081798-42081817 None:intergenic 35.0%
ATTCGATTGTGAAACTAACC+TGG + chr5_3:42082017-42082036 Msa0812160:intron 35.0%
TTCATTGTGAGTAGTGAATC+TGG + chr5_3:42082160-42082179 Msa0812160:five_prime_UTR 35.0%
AATTGAGAAAGAAGAATGGC+GGG + chr5_3:42082184-42082203 Msa0812160:exon 35.0%
TAAGATGTTGATCATAAGCC+TGG - chr5_3:42082384-42082403 None:intergenic 35.0%
!!! AGGTGTTTTAAGGTTTTGAC+TGG + chr5_3:42082613-42082632 Msa0812160:intron 35.0%
!!! GGTGTTTTAAGGTTTTGACT+GGG + chr5_3:42082614-42082633 Msa0812160:intron 35.0%
! ATATCTACTAACTTGTGACC+TGG + chr5_3:42082779-42082798 Msa0812160:intron 35.0%
ACTTGGTCAAACAAATAACC+AGG - chr5_3:42082800-42082819 None:intergenic 35.0%
!! TCCAAGGAAATTTTGATGGT+TGG - chr5_3:42082895-42082914 None:intergenic 35.0%
TCTAATTGCAGACTACAAAG+CGG + chr5_3:42082931-42082950 Msa0812160:intron 35.0%
! AGTTCCGTTCCTTTTTGTTA+GGG + chr5_3:42082956-42082975 Msa0812160:CDS 35.0%
TCTCATGCTAAAAAGAGAAC+AGG - chr5_3:42083174-42083193 None:intergenic 35.0%
ATCATTGGCGTTTAAGCTTA+TGG - chr5_3:42083266-42083285 None:intergenic 35.0%
AGGAAGTCTAGAAACTTTGA+AGG - chr5_3:42083385-42083404 None:intergenic 35.0%
CTAAACCAAACTTAGGATTC+AGG - chr5_3:42083405-42083424 None:intergenic 35.0%
GGAAAAGCTAAACCAAACTT+AGG - chr5_3:42083412-42083431 None:intergenic 35.0%
! AGGGCTTTTCAATTCAAGCT+TGG + chr5_3:42081912-42081931 Msa0812160:intron 40.0%
TCAATTCAAGCTTGGATTCG+AGG + chr5_3:42081920-42081939 Msa0812160:intron 40.0%
CAATTCAAGCTTGGATTCGA+GGG + chr5_3:42081921-42081940 Msa0812160:intron 40.0%
ATTGAGAAAGAAGAATGGCG+GGG + chr5_3:42082185-42082204 Msa0812160:exon 40.0%
!! AGAACTATGAGGCATTGACA+CGG + chr5_3:42082648-42082667 Msa0812160:intron 40.0%
GCCAACCATCAAAATTTCCT+TGG + chr5_3:42082891-42082910 Msa0812160:intron 40.0%
! CAGTTCCGTTCCTTTTTGTT+AGG + chr5_3:42082955-42082974 Msa0812160:CDS 40.0%
! GTTCCGTTCCTTTTTGTTAG+GGG + chr5_3:42082957-42082976 Msa0812160:CDS 40.0%
TCTCCCCTAACAAAAAGGAA+CGG - chr5_3:42082963-42082982 None:intergenic 40.0%
! TTCCTTTTTGTTAGGGGAGA+TGG + chr5_3:42082963-42082982 Msa0812160:CDS 40.0%
ACTAGCAAACATGCATGTTG+AGG - chr5_3:42083127-42083146 None:intergenic 40.0%
TCATGGAATGCTCATGAACA+AGG + chr5_3:42083195-42083214 Msa0812160:three_prime_UTR 40.0%
CATGGAATGCTCATGAACAA+GGG + chr5_3:42083196-42083215 Msa0812160:three_prime_UTR 40.0%
GAGCTGTTGTTACATAGATG+TGG + chr5_3:42083238-42083257 Msa0812160:three_prime_UTR 40.0%
TACATTGGAGATCTTGAACC+TGG + chr5_3:42083340-42083359 Msa0812160:three_prime_UTR 40.0%
ACATTGGAGATCTTGAACCT+GGG + chr5_3:42083341-42083360 Msa0812160:three_prime_UTR 40.0%
ATACAACATTCAAACACCCC+AGG - chr5_3:42083361-42083380 None:intergenic 40.0%
GACTTCCTGAATCCTAAGTT+TGG + chr5_3:42083397-42083416 Msa0812160:three_prime_UTR 40.0%
!! ACCAGAAAGAGCTTTGAGTT+GGG - chr5_3:42083433-42083452 None:intergenic 40.0%
! AGGGCTTTTCAATTCAAGCT+TGG + chr5_3:42081912-42081931 Msa0812160:intron 40.0%
TCAATTCAAGCTTGGATTCG+AGG + chr5_3:42081920-42081939 Msa0812160:intron 40.0%
CAATTCAAGCTTGGATTCGA+GGG + chr5_3:42081921-42081940 Msa0812160:intron 40.0%
ATTGAGAAAGAAGAATGGCG+GGG + chr5_3:42082185-42082204 Msa0812160:exon 40.0%
!! AGAACTATGAGGCATTGACA+CGG + chr5_3:42082648-42082667 Msa0812160:intron 40.0%
GCCAACCATCAAAATTTCCT+TGG + chr5_3:42082891-42082910 Msa0812160:intron 40.0%
! CAGTTCCGTTCCTTTTTGTT+AGG + chr5_3:42082955-42082974 Msa0812160:CDS 40.0%
! GTTCCGTTCCTTTTTGTTAG+GGG + chr5_3:42082957-42082976 Msa0812160:CDS 40.0%
TCTCCCCTAACAAAAAGGAA+CGG - chr5_3:42082963-42082982 None:intergenic 40.0%
! TTCCTTTTTGTTAGGGGAGA+TGG + chr5_3:42082963-42082982 Msa0812160:CDS 40.0%
ACTAGCAAACATGCATGTTG+AGG - chr5_3:42083127-42083146 None:intergenic 40.0%
TCATGGAATGCTCATGAACA+AGG + chr5_3:42083195-42083214 Msa0812160:three_prime_UTR 40.0%
CATGGAATGCTCATGAACAA+GGG + chr5_3:42083196-42083215 Msa0812160:three_prime_UTR 40.0%
GAGCTGTTGTTACATAGATG+TGG + chr5_3:42083238-42083257 Msa0812160:three_prime_UTR 40.0%
TACATTGGAGATCTTGAACC+TGG + chr5_3:42083340-42083359 Msa0812160:three_prime_UTR 40.0%
ACATTGGAGATCTTGAACCT+GGG + chr5_3:42083341-42083360 Msa0812160:three_prime_UTR 40.0%
ATACAACATTCAAACACCCC+AGG - chr5_3:42083361-42083380 None:intergenic 40.0%
GACTTCCTGAATCCTAAGTT+TGG + chr5_3:42083397-42083416 Msa0812160:three_prime_UTR 40.0%
!! ACCAGAAAGAGCTTTGAGTT+GGG - chr5_3:42083433-42083452 None:intergenic 40.0%
ATCTCTCCATCGCTTCAACA+AGG + chr5_3:42081763-42081782 Msa0812160:five_prime_UTR 45.0%
CTGGGTAATTCACAAACACC+AGG - chr5_3:42082038-42082057 None:intergenic 45.0%
CTGGTGTTTGTGAATTACCC+AGG + chr5_3:42082036-42082055 Msa0812160:intron 45.0%
AGGAGCCTTTGGATCTCATA+AGG + chr5_3:42082230-42082249 Msa0812160:CDS 45.0%
! CTCAGCCTTATGAGATCCAA+AGG - chr5_3:42082238-42082257 None:intergenic 45.0%
CACGAAGCTCTCTATCAGAA+CGG - chr5_3:42082288-42082307 None:intergenic 45.0%
!! GAACTATGAGGCATTGACAC+GGG + chr5_3:42082649-42082668 Msa0812160:intron 45.0%
!! TTTTGATGGTTGGCGCAGAT+TGG - chr5_3:42082885-42082904 None:intergenic 45.0%
!! TATTGGTTTCTCCACCACTG+AGG + chr5_3:42082991-42083010 Msa0812160:CDS 45.0%
GGGTTATACTTATGGCAGCT+TGG + chr5_3:42083216-42083235 Msa0812160:three_prime_UTR 45.0%
CATTGGAGATCTTGAACCTG+GGG + chr5_3:42083342-42083361 Msa0812160:three_prime_UTR 45.0%
TCCCAACTCAAAGCTCTTTC+TGG + chr5_3:42083429-42083448 Msa0812160:three_prime_UTR 45.0%
!! GACCAGAAAGAGCTTTGAGT+TGG - chr5_3:42083434-42083453 None:intergenic 45.0%
ATCTCTCCATCGCTTCAACA+AGG + chr5_3:42081763-42081782 Msa0812160:five_prime_UTR 45.0%
CTGGGTAATTCACAAACACC+AGG - chr5_3:42082038-42082057 None:intergenic 45.0%
CTGGTGTTTGTGAATTACCC+AGG + chr5_3:42082036-42082055 Msa0812160:intron 45.0%
AGGAGCCTTTGGATCTCATA+AGG + chr5_3:42082230-42082249 Msa0812160:CDS 45.0%
! CTCAGCCTTATGAGATCCAA+AGG - chr5_3:42082238-42082257 None:intergenic 45.0%
CACGAAGCTCTCTATCAGAA+CGG - chr5_3:42082288-42082307 None:intergenic 45.0%
!! GAACTATGAGGCATTGACAC+GGG + chr5_3:42082649-42082668 Msa0812160:intron 45.0%
!! TTTTGATGGTTGGCGCAGAT+TGG - chr5_3:42082885-42082904 None:intergenic 45.0%
!! TATTGGTTTCTCCACCACTG+AGG + chr5_3:42082991-42083010 Msa0812160:CDS 45.0%
GGGTTATACTTATGGCAGCT+TGG + chr5_3:42083216-42083235 Msa0812160:three_prime_UTR 45.0%
CATTGGAGATCTTGAACCTG+GGG + chr5_3:42083342-42083361 Msa0812160:three_prime_UTR 45.0%
TCCCAACTCAAAGCTCTTTC+TGG + chr5_3:42083429-42083448 Msa0812160:three_prime_UTR 45.0%
!! GACCAGAAAGAGCTTTGAGT+TGG - chr5_3:42083434-42083453 None:intergenic 45.0%
!! AAAAATAAAATGTTATTATA+AGG + chr5_3:42082083-42082102 Msa0812160:intron 5.0%
!!! GTTTATTTTATTTATTTTTT+AGG + chr5_3:42082135-42082154 Msa0812160:intron 5.0%
!! AAAAATAAAATGTTATTATA+AGG + chr5_3:42082083-42082102 Msa0812160:intron 5.0%
!!! GTTTATTTTATTTATTTTTT+AGG + chr5_3:42082135-42082154 Msa0812160:intron 5.0%
TCTCCATCGCTTCAACAAGG+AGG + chr5_3:42081766-42081785 Msa0812160:five_prime_UTR 50.0%
GTACCTCCTTGTTGAAGCGA+TGG - chr5_3:42081772-42081791 None:intergenic 50.0%
! AGAGGAAGAAAGCGCAGTGA+AGG + chr5_3:42082210-42082229 Msa0812160:CDS 50.0%
CGTTCTGATAGAGAGCTTCG+TGG + chr5_3:42082286-42082305 Msa0812160:CDS 50.0%
CGCCATCTCCCCTAACAAAA+AGG - chr5_3:42082968-42082987 None:intergenic 50.0%
TAGGGGAGATGGCGTGATAT+TGG + chr5_3:42082974-42082993 Msa0812160:CDS 50.0%
TGTTATGCAGTCCTCAGTGG+TGG - chr5_3:42083005-42083024 None:intergenic 50.0%
CCATGTTATGCAGTCCTCAG+TGG - chr5_3:42083008-42083027 None:intergenic 50.0%
CCACTGAGGACTGCATAACA+TGG + chr5_3:42083005-42083024 Msa0812160:exon 50.0%
TCTCCATCGCTTCAACAAGG+AGG + chr5_3:42081766-42081785 Msa0812160:five_prime_UTR 50.0%
GTACCTCCTTGTTGAAGCGA+TGG - chr5_3:42081772-42081791 None:intergenic 50.0%
! AGAGGAAGAAAGCGCAGTGA+AGG + chr5_3:42082210-42082229 Msa0812160:CDS 50.0%
CGTTCTGATAGAGAGCTTCG+TGG + chr5_3:42082286-42082305 Msa0812160:CDS 50.0%
CGCCATCTCCCCTAACAAAA+AGG - chr5_3:42082968-42082987 None:intergenic 50.0%
TAGGGGAGATGGCGTGATAT+TGG + chr5_3:42082974-42082993 Msa0812160:CDS 50.0%
TGTTATGCAGTCCTCAGTGG+TGG - chr5_3:42083005-42083024 None:intergenic 50.0%
CCATGTTATGCAGTCCTCAG+TGG - chr5_3:42083008-42083027 None:intergenic 50.0%
CCACTGAGGACTGCATAACA+TGG + chr5_3:42083005-42083024 Msa0812160:exon 50.0%
! AAGAAGAATGGCGGGGACAG+AGG + chr5_3:42082192-42082211 Msa0812160:exon 55.0%
AAGCGCAGTGAAGGAGCCTT+TGG + chr5_3:42082219-42082238 Msa0812160:CDS 55.0%
! AAGAAGAATGGCGGGGACAG+AGG + chr5_3:42082192-42082211 Msa0812160:exon 55.0%
AAGCGCAGTGAAGGAGCCTT+TGG + chr5_3:42082219-42082238 Msa0812160:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 42081755 42083460 42081755 ID=Msa0812160;Name=Msa0812160
chr5_3 mRNA 42081755 42083460 42081755 ID=Msa0812160-mRNA-1;Parent=Msa0812160;Name=Msa0812160-mRNA-1;_AED=0.05;_eAED=0.05;_QI=75|1|1|1|0.66|0.5|4|438|98
chr5_3 exon 42081755 42081787 42081755 ID=Msa0812160-mRNA-1:exon:12864;Parent=Msa0812160-mRNA-1
chr5_3 exon 42082157 42082318 42082157 ID=Msa0812160-mRNA-1:exon:12865;Parent=Msa0812160-mRNA-1
chr5_3 exon 42082385 42082480 42082385 ID=Msa0812160-mRNA-1:exon:12866;Parent=Msa0812160-mRNA-1
chr5_3 exon 42082942 42083460 42082942 ID=Msa0812160-mRNA-1:exon:12867;Parent=Msa0812160-mRNA-1
chr5_3 five_prime_UTR 42081755 42081787 42081755 ID=Msa0812160-mRNA-1:five_prime_utr;Parent=Msa0812160-mRNA-1
chr5_3 five_prime_UTR 42082157 42082198 42082157 ID=Msa0812160-mRNA-1:five_prime_utr;Parent=Msa0812160-mRNA-1
chr5_3 CDS 42082199 42082318 42082199 ID=Msa0812160-mRNA-1:cds;Parent=Msa0812160-mRNA-1
chr5_3 CDS 42082385 42082480 42082385 ID=Msa0812160-mRNA-1:cds;Parent=Msa0812160-mRNA-1
chr5_3 CDS 42082942 42083022 42082942 ID=Msa0812160-mRNA-1:cds;Parent=Msa0812160-mRNA-1
chr5_3 three_prime_UTR 42083023 42083460 42083023 ID=Msa0812160-mRNA-1:three_prime_utr;Parent=Msa0812160-mRNA-1
Gene Sequence

>Msa0812160

ATGGCGGGGACAGAGGAAGAAAGCGCAGTGAAGGAGCCTTTGGATCTCATAAGGCTGAGTCTCGACGAACGTATCTACGTTAAACTCCGTTCTGATAGAGAGCTTCGTGGAAAACTTCATGCTTATGATCAACATCTTAATATGATTCTTGGTGATGTTGAAGAAATTGTTACTACTGTTGAAATTGATGATGAGACATATGAAGAAATTGTCAGGACTACAAAGCGGACAGTTCCGTTCCTTTTTGTTAGGGGAGATGGCGTGATATTGGTTTCTCCACCACTGAGGACTGCATAA
Protein sequence

>Msa0812160

MAGTEEESAVKEPLDLIRLSLDERIYVKLRSDRELRGKLHAYDQHLNMILGDVEEIVTTVEIDDETYEEIVRTTKRTVPFLFVRGDGVILVSPPLRTA*