AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0852430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0852430 MtrunA17_Chr4g0043221 100.000 98 0 0 1 98 1 98 2.34e-66 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0852430 AT1G76860.1 89.796 98 9 1 1 98 1 97 9.87e-59 175
Msa0852430 AT1G21190.1 86.458 96 13 0 3 98 2 97 8.05e-58 173

Find 51 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAACTCAAAGCGTCTTTC+TGG 0.177888 5_4:+43481935 Msa0852430:three_prime_UTR
ATCATTTGCGTTTAAGCTTA+TGG 0.183692 5_4:-43481766 None:intergenic
CAACATCTTAATATGATTCT+TGG 0.270259 5_4:+43480897 Msa0852430:CDS
CGCCATCTCCCCTAACAAAA+AGG 0.290294 5_4:-43481468 None:intergenic
GACTTCCTGAATCCTAAGTT+TGG 0.356983 5_4:+43481901 Msa0852430:three_prime_UTR
CTAAACCAAACTTAGGATTC+AGG 0.374091 5_4:-43481906 None:intergenic
CCAGAAAGACGCTTTGAGTT+GGG 0.377597 5_4:-43481935 None:intergenic
ATGAACAAGGGTTATACTTA+TGG 0.392555 5_4:+43481711 Msa0852430:three_prime_UTR
TAGGGGAGATGGCGTGATAT+TGG 0.403792 5_4:+43481477 Msa0852430:CDS
TCATTGTGAGTAGTGGAATC+TGG 0.417049 5_4:+43480648 Msa0852430:five_prime_UTR
AGACATATGAAGAAATTGTC+AGG 0.427463 5_4:+43480961 Msa0852430:CDS
AAGCGCAGTGAAGGAGCCTT+TGG 0.436284 5_4:+43480707 Msa0852430:CDS
CTTCTAAGTTCTAGTTACAT+TGG 0.450409 5_4:+43481829 Msa0852430:three_prime_UTR
AGGAAGTCTACAAACTTTGA+AGG 0.454963 5_4:-43481886 None:intergenic
GGAAAAGCTAAACCAAACTT+AGG 0.457822 5_4:-43481913 None:intergenic
TACATTGGAGATCTTGAACC+TGG 0.462550 5_4:+43481844 Msa0852430:three_prime_UTR
GAGGATAATCTAATATCATA+AGG 0.485064 5_4:-43481608 None:intergenic
AGAGGAAGAAAGCGCAGTGA+AGG 0.494057 5_4:+43480698 Msa0852430:CDS
ACATTGGAGATCTTGAACCT+GGG 0.500499 5_4:+43481845 Msa0852430:three_prime_UTR
ATGAGTGAGTTTGAGACAGT+TGG 0.507686 5_4:-43480417 None:intergenic
TAGGGTTTCATTGTGAGTAG+TGG 0.510204 5_4:+43480641 Msa0852430:intron
ACCAGAAAGACGCTTTGAGT+TGG 0.519386 5_4:-43481936 None:intergenic
AGGAGCCTTTGGATCTCATA+AGG 0.521514 5_4:+43480718 Msa0852430:CDS
AAGAAGAATGGCGGGGACAG+AGG 0.529954 5_4:+43480680 Msa0852430:exon
CCTCCTTGTTGTTGAAGCGA+TGG 0.532698 5_4:-43480483 None:intergenic
CAGAAAGACGCTTTGAGTTG+GGG 0.538573 5_4:-43481934 None:intergenic
TCATGGAATGCTCATGAACA+AGG 0.539148 5_4:+43481698 Msa0852430:three_prime_UTR
AATTGAGAAAGAAGAATGGC+GGG 0.544172 5_4:+43480672 Msa0852430:exon
CGTTCTGATAGAGAGCTTCG+TGG 0.548358 5_4:+43480774 Msa0852430:CDS
CTCAGCCTTATGAGATCCAA+AGG 0.555367 5_4:-43480723 None:intergenic
GAGCTGTTGTTACATAGATG+TGG 0.561086 5_4:+43481741 Msa0852430:three_prime_UTR
CCACTGAGGACTGCATAACA+TGG 0.569381 5_4:+43481508 Msa0852430:exon
GGGTTATACTTATGGCAGCT+TGG 0.573830 5_4:+43481719 Msa0852430:three_prime_UTR
TGAGTGAGTTTGAGACAGTT+GGG 0.575957 5_4:-43480416 None:intergenic
TGGTAATTGAGAAAGAAGAA+TGG 0.601575 5_4:+43480668 Msa0852430:exon
CATTGGAGATCTTGAACCTG+GGG 0.601867 5_4:+43481846 Msa0852430:three_prime_UTR
TCTCCATCGCTTCAACAACA+AGG 0.616871 5_4:+43480480 Msa0852430:five_prime_UTR
ACTAGCAAACATGCATGTTG+AGG 0.616942 5_4:-43481627 None:intergenic
CACGAAGCTCTCTATCAGAA+CGG 0.619250 5_4:-43480773 None:intergenic
CCATGTTATGCAGTCCTCAG+TGG 0.619717 5_4:-43481508 None:intergenic
GACATATGAAGAAATTGTCA+GGG 0.620564 5_4:+43480962 Msa0852430:CDS
TGTTATGCAGTCCTCAGTGG+TGG 0.628383 5_4:-43481505 None:intergenic
AAAGAGAGAAACAAAGAATG+CGG 0.659823 5_4:-43480443 None:intergenic
TAATTGAGAAAGAAGAATGG+CGG 0.660632 5_4:+43480671 Msa0852430:exon
CATGGAATGCTCATGAACAA+GGG 0.663566 5_4:+43481699 Msa0852430:three_prime_UTR
ATACAACATTCAAACACCCC+AGG 0.667078 5_4:-43481862 None:intergenic
TCTAATTGCAGACTACAAAG+CGG 0.689189 5_4:+43481434 Msa0852430:intron
ATCTAATATCATAAGGAACG+AGG 0.696193 5_4:-43481601 None:intergenic
ATTGAGAAAGAAGAATGGCG+GGG 0.698364 5_4:+43480673 Msa0852430:exon
TATTGGTTTCTCCACCACTG+AGG 0.734600 5_4:+43481494 Msa0852430:CDS
CCATCGCTTCAACAACAAGG+AGG 0.745321 5_4:+43480483 Msa0852430:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTATTTATTTTTT+AGG + chr5_4:43480622-43480641 Msa0852430:intron 0.0%
!!! TTTTATTTATTTATTTTTTA+GGG + chr5_4:43480623-43480642 Msa0852430:intron 0.0%
!!! ATTTTATTTATTTATTTTTT+AGG + chr5_4:43480622-43480641 Msa0852430:intron 0.0%
!!! TTTTATTTATTTATTTTTTA+GGG + chr5_4:43480623-43480642 Msa0852430:intron 0.0%
!! TAAAAGATTATTGTAGTTTA+AGG + chr5_4:43481096-43481115 Msa0852430:intron 15.0%
!!! TATTTTTTAGATTAGATTAC+TGG + chr5_4:43481218-43481237 Msa0852430:intron 15.0%
!! TAAAAGATTATTGTAGTTTA+AGG + chr5_4:43481096-43481115 Msa0852430:intron 15.0%
!!! TATTTTTTAGATTAGATTAC+TGG + chr5_4:43481218-43481237 Msa0852430:intron 15.0%
!!! TTAATTAATAAGCATTGTTG+AGG + chr5_4:43481034-43481053 Msa0852430:intron 20.0%
!!! TAATTAATAAGCATTGTTGA+GGG + chr5_4:43481035-43481054 Msa0852430:intron 20.0%
!!! AAGTTATAGAATTTTTGTGA+TGG - chr5_4:43481569-43481588 None:intergenic 20.0%
!!! TTAATTAATAAGCATTGTTG+AGG + chr5_4:43481034-43481053 Msa0852430:intron 20.0%
!!! TAATTAATAAGCATTGTTGA+GGG + chr5_4:43481035-43481054 Msa0852430:intron 20.0%
!!! AAGTTATAGAATTTTTGTGA+TGG - chr5_4:43481569-43481588 None:intergenic 20.0%
! TGAAAGAAAAAAGAGATGAA+AGG - chr5_4:43480829-43480848 None:intergenic 25.0%
! CAATTTCAATTTCAATTTCC+AGG + chr5_4:43480866-43480885 Msa0852430:intron 25.0%
! CAACATCTTAATATGATTCT+TGG + chr5_4:43480897-43480916 Msa0852430:CDS 25.0%
!! TACTTTCTACAATTTAGCTT+TGG - chr5_4:43481004-43481023 None:intergenic 25.0%
!! ATCTTTTATCAAACTGAAGA+AGG - chr5_4:43481084-43481103 None:intergenic 25.0%
!!! TTGTAGTTTAAGGTGTTTTA+AGG + chr5_4:43481106-43481125 Msa0852430:intron 25.0%
! ATCACAGTTGTGAAAAAATA+AGG - chr5_4:43481264-43481283 None:intergenic 25.0%
! CCAATAAAACAATAGAAACT+TGG - chr5_4:43481320-43481339 None:intergenic 25.0%
!!! CCAAGTTTCTATTGTTTTAT+TGG + chr5_4:43481317-43481336 Msa0852430:intron 25.0%
! AGAAAAACAAAAATCATCCA+AGG - chr5_4:43481414-43481433 None:intergenic 25.0%
! GAGGATAATCTAATATCATA+AGG - chr5_4:43481611-43481630 None:intergenic 25.0%
! TGAAAGAAAAAAGAGATGAA+AGG - chr5_4:43480829-43480848 None:intergenic 25.0%
! CAATTTCAATTTCAATTTCC+AGG + chr5_4:43480866-43480885 Msa0852430:intron 25.0%
! CAACATCTTAATATGATTCT+TGG + chr5_4:43480897-43480916 Msa0852430:CDS 25.0%
!! TACTTTCTACAATTTAGCTT+TGG - chr5_4:43481004-43481023 None:intergenic 25.0%
!! ATCTTTTATCAAACTGAAGA+AGG - chr5_4:43481084-43481103 None:intergenic 25.0%
!!! TTGTAGTTTAAGGTGTTTTA+AGG + chr5_4:43481106-43481125 Msa0852430:intron 25.0%
! ATCACAGTTGTGAAAAAATA+AGG - chr5_4:43481264-43481283 None:intergenic 25.0%
! CCAATAAAACAATAGAAACT+TGG - chr5_4:43481320-43481339 None:intergenic 25.0%
!!! CCAAGTTTCTATTGTTTTAT+TGG + chr5_4:43481317-43481336 Msa0852430:intron 25.0%
! AGAAAAACAAAAATCATCCA+AGG - chr5_4:43481414-43481433 None:intergenic 25.0%
! GAGGATAATCTAATATCATA+AGG - chr5_4:43481611-43481630 None:intergenic 25.0%
AAAGAGAGAAACAAAGAATG+CGG - chr5_4:43480446-43480465 None:intergenic 30.0%
!! TGTGTTGTGTTAATTTGTGA+AGG + chr5_4:43480596-43480615 Msa0852430:intron 30.0%
TGGTAATTGAGAAAGAAGAA+TGG + chr5_4:43480668-43480687 Msa0852430:exon 30.0%
TAATTGAGAAAGAAGAATGG+CGG + chr5_4:43480671-43480690 Msa0852430:exon 30.0%
AGACATATGAAGAAATTGTC+AGG + chr5_4:43480961-43480980 Msa0852430:CDS 30.0%
GACATATGAAGAAATTGTCA+GGG + chr5_4:43480962-43480981 Msa0852430:CDS 30.0%
ACTCTGATTTAAGAACTATG+AGG + chr5_4:43481140-43481159 Msa0852430:intron 30.0%
AGGTCAATAGTTGAAAGAAA+AGG - chr5_4:43481244-43481263 None:intergenic 30.0%
! ATCATCCAAGGAAATTTTGA+TGG - chr5_4:43481402-43481421 None:intergenic 30.0%
ATCTAATATCATAAGGAACG+AGG - chr5_4:43481604-43481623 None:intergenic 30.0%
!! TCTTTTTAGCATGAGAATCA+TGG + chr5_4:43481681-43481700 Msa0852430:three_prime_UTR 30.0%
ATGAACAAGGGTTATACTTA+TGG + chr5_4:43481711-43481730 Msa0852430:three_prime_UTR 30.0%
ATCATTTGCGTTTAAGCTTA+TGG - chr5_4:43481769-43481788 None:intergenic 30.0%
! CTTCTAAGTTCTAGTTACAT+TGG + chr5_4:43481829-43481848 Msa0852430:three_prime_UTR 30.0%
AAAGAGAGAAACAAAGAATG+CGG - chr5_4:43480446-43480465 None:intergenic 30.0%
!! TGTGTTGTGTTAATTTGTGA+AGG + chr5_4:43480596-43480615 Msa0852430:intron 30.0%
TGGTAATTGAGAAAGAAGAA+TGG + chr5_4:43480668-43480687 Msa0852430:exon 30.0%
TAATTGAGAAAGAAGAATGG+CGG + chr5_4:43480671-43480690 Msa0852430:exon 30.0%
AGACATATGAAGAAATTGTC+AGG + chr5_4:43480961-43480980 Msa0852430:CDS 30.0%
GACATATGAAGAAATTGTCA+GGG + chr5_4:43480962-43480981 Msa0852430:CDS 30.0%
ACTCTGATTTAAGAACTATG+AGG + chr5_4:43481140-43481159 Msa0852430:intron 30.0%
AGGTCAATAGTTGAAAGAAA+AGG - chr5_4:43481244-43481263 None:intergenic 30.0%
! ATCATCCAAGGAAATTTTGA+TGG - chr5_4:43481402-43481421 None:intergenic 30.0%
ATCTAATATCATAAGGAACG+AGG - chr5_4:43481604-43481623 None:intergenic 30.0%
!! TCTTTTTAGCATGAGAATCA+TGG + chr5_4:43481681-43481700 Msa0852430:three_prime_UTR 30.0%
ATGAACAAGGGTTATACTTA+TGG + chr5_4:43481711-43481730 Msa0852430:three_prime_UTR 30.0%
ATCATTTGCGTTTAAGCTTA+TGG - chr5_4:43481769-43481788 None:intergenic 30.0%
! CTTCTAAGTTCTAGTTACAT+TGG + chr5_4:43481829-43481848 Msa0852430:three_prime_UTR 30.0%
AGATTGAAAGAGAAAGATCG+AGG - chr5_4:43480515-43480534 None:intergenic 35.0%
AATTGAGAAAGAAGAATGGC+GGG + chr5_4:43480672-43480691 Msa0852430:exon 35.0%
TAAGATGTTGATCATAAGCC+TGG - chr5_4:43480887-43480906 None:intergenic 35.0%
!!! AGGTGTTTTAAGGTTTTGAC+TGG + chr5_4:43481116-43481135 Msa0852430:intron 35.0%
!!! GGTGTTTTAAGGTTTTGACT+GGG + chr5_4:43481117-43481136 Msa0852430:intron 35.0%
! ATATCTACTAACTTGTGACC+TGG + chr5_4:43481282-43481301 Msa0852430:intron 35.0%
ACTTGGTCAAACAAATAACC+AGG - chr5_4:43481303-43481322 None:intergenic 35.0%
!! TCCAAGGAAATTTTGATGGT+TGG - chr5_4:43481398-43481417 None:intergenic 35.0%
TCTAATTGCAGACTACAAAG+CGG + chr5_4:43481434-43481453 Msa0852430:intron 35.0%
! AGTTCCGTTCCTTTTTGTTA+GGG + chr5_4:43481459-43481478 Msa0852430:CDS 35.0%
TCTCATGCTAAAAAGAGAAC+AGG - chr5_4:43481677-43481696 None:intergenic 35.0%
AGGAAGTCTACAAACTTTGA+AGG - chr5_4:43481889-43481908 None:intergenic 35.0%
CTAAACCAAACTTAGGATTC+AGG - chr5_4:43481909-43481928 None:intergenic 35.0%
GGAAAAGCTAAACCAAACTT+AGG - chr5_4:43481916-43481935 None:intergenic 35.0%
AGATTGAAAGAGAAAGATCG+AGG - chr5_4:43480515-43480534 None:intergenic 35.0%
AATTGAGAAAGAAGAATGGC+GGG + chr5_4:43480672-43480691 Msa0852430:exon 35.0%
TAAGATGTTGATCATAAGCC+TGG - chr5_4:43480887-43480906 None:intergenic 35.0%
!!! AGGTGTTTTAAGGTTTTGAC+TGG + chr5_4:43481116-43481135 Msa0852430:intron 35.0%
!!! GGTGTTTTAAGGTTTTGACT+GGG + chr5_4:43481117-43481136 Msa0852430:intron 35.0%
! ATATCTACTAACTTGTGACC+TGG + chr5_4:43481282-43481301 Msa0852430:intron 35.0%
ACTTGGTCAAACAAATAACC+AGG - chr5_4:43481303-43481322 None:intergenic 35.0%
!! TCCAAGGAAATTTTGATGGT+TGG - chr5_4:43481398-43481417 None:intergenic 35.0%
TCTAATTGCAGACTACAAAG+CGG + chr5_4:43481434-43481453 Msa0852430:intron 35.0%
! AGTTCCGTTCCTTTTTGTTA+GGG + chr5_4:43481459-43481478 Msa0852430:CDS 35.0%
TCTCATGCTAAAAAGAGAAC+AGG - chr5_4:43481677-43481696 None:intergenic 35.0%
AGGAAGTCTACAAACTTTGA+AGG - chr5_4:43481889-43481908 None:intergenic 35.0%
CTAAACCAAACTTAGGATTC+AGG - chr5_4:43481909-43481928 None:intergenic 35.0%
GGAAAAGCTAAACCAAACTT+AGG - chr5_4:43481916-43481935 None:intergenic 35.0%
! TGAGTGAGTTTGAGACAGTT+GGG - chr5_4:43480419-43480438 None:intergenic 40.0%
! ATGAGTGAGTTTGAGACAGT+TGG - chr5_4:43480420-43480439 None:intergenic 40.0%
TAGGGTTTCATTGTGAGTAG+TGG + chr5_4:43480641-43480660 Msa0852430:intron 40.0%
! TCATTGTGAGTAGTGGAATC+TGG + chr5_4:43480648-43480667 Msa0852430:five_prime_UTR 40.0%
ATTGAGAAAGAAGAATGGCG+GGG + chr5_4:43480673-43480692 Msa0852430:exon 40.0%
GCCAACCATCAAAATTTCCT+TGG + chr5_4:43481394-43481413 Msa0852430:intron 40.0%
! CAGTTCCGTTCCTTTTTGTT+AGG + chr5_4:43481458-43481477 Msa0852430:CDS 40.0%
! GTTCCGTTCCTTTTTGTTAG+GGG + chr5_4:43481460-43481479 Msa0852430:CDS 40.0%
TCTCCCCTAACAAAAAGGAA+CGG - chr5_4:43481466-43481485 None:intergenic 40.0%
! TTCCTTTTTGTTAGGGGAGA+TGG + chr5_4:43481466-43481485 Msa0852430:CDS 40.0%
ACTAGCAAACATGCATGTTG+AGG - chr5_4:43481630-43481649 None:intergenic 40.0%
TCATGGAATGCTCATGAACA+AGG + chr5_4:43481698-43481717 Msa0852430:three_prime_UTR 40.0%
CATGGAATGCTCATGAACAA+GGG + chr5_4:43481699-43481718 Msa0852430:three_prime_UTR 40.0%
GAGCTGTTGTTACATAGATG+TGG + chr5_4:43481741-43481760 Msa0852430:three_prime_UTR 40.0%
TACATTGGAGATCTTGAACC+TGG + chr5_4:43481844-43481863 Msa0852430:three_prime_UTR 40.0%
ACATTGGAGATCTTGAACCT+GGG + chr5_4:43481845-43481864 Msa0852430:three_prime_UTR 40.0%
ATACAACATTCAAACACCCC+AGG - chr5_4:43481865-43481884 None:intergenic 40.0%
GACTTCCTGAATCCTAAGTT+TGG + chr5_4:43481901-43481920 Msa0852430:three_prime_UTR 40.0%
! TGAGTGAGTTTGAGACAGTT+GGG - chr5_4:43480419-43480438 None:intergenic 40.0%
! ATGAGTGAGTTTGAGACAGT+TGG - chr5_4:43480420-43480439 None:intergenic 40.0%
TAGGGTTTCATTGTGAGTAG+TGG + chr5_4:43480641-43480660 Msa0852430:intron 40.0%
! TCATTGTGAGTAGTGGAATC+TGG + chr5_4:43480648-43480667 Msa0852430:five_prime_UTR 40.0%
ATTGAGAAAGAAGAATGGCG+GGG + chr5_4:43480673-43480692 Msa0852430:exon 40.0%
GCCAACCATCAAAATTTCCT+TGG + chr5_4:43481394-43481413 Msa0852430:intron 40.0%
! CAGTTCCGTTCCTTTTTGTT+AGG + chr5_4:43481458-43481477 Msa0852430:CDS 40.0%
! GTTCCGTTCCTTTTTGTTAG+GGG + chr5_4:43481460-43481479 Msa0852430:CDS 40.0%
TCTCCCCTAACAAAAAGGAA+CGG - chr5_4:43481466-43481485 None:intergenic 40.0%
! TTCCTTTTTGTTAGGGGAGA+TGG + chr5_4:43481466-43481485 Msa0852430:CDS 40.0%
ACTAGCAAACATGCATGTTG+AGG - chr5_4:43481630-43481649 None:intergenic 40.0%
TCATGGAATGCTCATGAACA+AGG + chr5_4:43481698-43481717 Msa0852430:three_prime_UTR 40.0%
CATGGAATGCTCATGAACAA+GGG + chr5_4:43481699-43481718 Msa0852430:three_prime_UTR 40.0%
GAGCTGTTGTTACATAGATG+TGG + chr5_4:43481741-43481760 Msa0852430:three_prime_UTR 40.0%
TACATTGGAGATCTTGAACC+TGG + chr5_4:43481844-43481863 Msa0852430:three_prime_UTR 40.0%
ACATTGGAGATCTTGAACCT+GGG + chr5_4:43481845-43481864 Msa0852430:three_prime_UTR 40.0%
ATACAACATTCAAACACCCC+AGG - chr5_4:43481865-43481884 None:intergenic 40.0%
GACTTCCTGAATCCTAAGTT+TGG + chr5_4:43481901-43481920 Msa0852430:three_prime_UTR 40.0%
TCTCCATCGCTTCAACAACA+AGG + chr5_4:43480480-43480499 Msa0852430:five_prime_UTR 45.0%
AGGAGCCTTTGGATCTCATA+AGG + chr5_4:43480718-43480737 Msa0852430:CDS 45.0%
! CTCAGCCTTATGAGATCCAA+AGG - chr5_4:43480726-43480745 None:intergenic 45.0%
CACGAAGCTCTCTATCAGAA+CGG - chr5_4:43480776-43480795 None:intergenic 45.0%
!! GAACTATGAGGCATTGACAC+AGG + chr5_4:43481152-43481171 Msa0852430:intron 45.0%
!! TTTTGATGGTTGGCGCAGAT+TGG - chr5_4:43481388-43481407 None:intergenic 45.0%
!! TATTGGTTTCTCCACCACTG+AGG + chr5_4:43481494-43481513 Msa0852430:CDS 45.0%
GGGTTATACTTATGGCAGCT+TGG + chr5_4:43481719-43481738 Msa0852430:three_prime_UTR 45.0%
CATTGGAGATCTTGAACCTG+GGG + chr5_4:43481846-43481865 Msa0852430:three_prime_UTR 45.0%
!! CAGAAAGACGCTTTGAGTTG+GGG - chr5_4:43481937-43481956 None:intergenic 45.0%
! CCAGAAAGACGCTTTGAGTT+GGG - chr5_4:43481938-43481957 None:intergenic 45.0%
! ACCAGAAAGACGCTTTGAGT+TGG - chr5_4:43481939-43481958 None:intergenic 45.0%
TCTCCATCGCTTCAACAACA+AGG + chr5_4:43480480-43480499 Msa0852430:five_prime_UTR 45.0%
AGGAGCCTTTGGATCTCATA+AGG + chr5_4:43480718-43480737 Msa0852430:CDS 45.0%
! CTCAGCCTTATGAGATCCAA+AGG - chr5_4:43480726-43480745 None:intergenic 45.0%
CACGAAGCTCTCTATCAGAA+CGG - chr5_4:43480776-43480795 None:intergenic 45.0%
!! GAACTATGAGGCATTGACAC+AGG + chr5_4:43481152-43481171 Msa0852430:intron 45.0%
!! TTTTGATGGTTGGCGCAGAT+TGG - chr5_4:43481388-43481407 None:intergenic 45.0%
!! TATTGGTTTCTCCACCACTG+AGG + chr5_4:43481494-43481513 Msa0852430:CDS 45.0%
GGGTTATACTTATGGCAGCT+TGG + chr5_4:43481719-43481738 Msa0852430:three_prime_UTR 45.0%
CATTGGAGATCTTGAACCTG+GGG + chr5_4:43481846-43481865 Msa0852430:three_prime_UTR 45.0%
!! CAGAAAGACGCTTTGAGTTG+GGG - chr5_4:43481937-43481956 None:intergenic 45.0%
! CCAGAAAGACGCTTTGAGTT+GGG - chr5_4:43481938-43481957 None:intergenic 45.0%
! ACCAGAAAGACGCTTTGAGT+TGG - chr5_4:43481939-43481958 None:intergenic 45.0%
!! AAAAATAAAATGTTATTATA+AGG + chr5_4:43480569-43480588 Msa0852430:intron 5.0%
!! AAAAATAAAATGTTATTATA+AGG + chr5_4:43480569-43480588 Msa0852430:intron 5.0%
!! CCTCCTTGTTGTTGAAGCGA+TGG - chr5_4:43480486-43480505 None:intergenic 50.0%
CCATCGCTTCAACAACAAGG+AGG + chr5_4:43480483-43480502 Msa0852430:five_prime_UTR 50.0%
! AGAGGAAGAAAGCGCAGTGA+AGG + chr5_4:43480698-43480717 Msa0852430:CDS 50.0%
CGTTCTGATAGAGAGCTTCG+TGG + chr5_4:43480774-43480793 Msa0852430:CDS 50.0%
CGCCATCTCCCCTAACAAAA+AGG - chr5_4:43481471-43481490 None:intergenic 50.0%
TAGGGGAGATGGCGTGATAT+TGG + chr5_4:43481477-43481496 Msa0852430:CDS 50.0%
TGTTATGCAGTCCTCAGTGG+TGG - chr5_4:43481508-43481527 None:intergenic 50.0%
CCATGTTATGCAGTCCTCAG+TGG - chr5_4:43481511-43481530 None:intergenic 50.0%
CCACTGAGGACTGCATAACA+TGG + chr5_4:43481508-43481527 Msa0852430:exon 50.0%
! CCCAACTCAAAGCGTCTTTC+TGG + chr5_4:43481935-43481954 Msa0852430:three_prime_UTR 50.0%
!! CCTCCTTGTTGTTGAAGCGA+TGG - chr5_4:43480486-43480505 None:intergenic 50.0%
CCATCGCTTCAACAACAAGG+AGG + chr5_4:43480483-43480502 Msa0852430:five_prime_UTR 50.0%
! AGAGGAAGAAAGCGCAGTGA+AGG + chr5_4:43480698-43480717 Msa0852430:CDS 50.0%
CGTTCTGATAGAGAGCTTCG+TGG + chr5_4:43480774-43480793 Msa0852430:CDS 50.0%
CGCCATCTCCCCTAACAAAA+AGG - chr5_4:43481471-43481490 None:intergenic 50.0%
TAGGGGAGATGGCGTGATAT+TGG + chr5_4:43481477-43481496 Msa0852430:CDS 50.0%
TGTTATGCAGTCCTCAGTGG+TGG - chr5_4:43481508-43481527 None:intergenic 50.0%
CCATGTTATGCAGTCCTCAG+TGG - chr5_4:43481511-43481530 None:intergenic 50.0%
CCACTGAGGACTGCATAACA+TGG + chr5_4:43481508-43481527 Msa0852430:exon 50.0%
! CCCAACTCAAAGCGTCTTTC+TGG + chr5_4:43481935-43481954 Msa0852430:three_prime_UTR 50.0%
! AAGAAGAATGGCGGGGACAG+AGG + chr5_4:43480680-43480699 Msa0852430:exon 55.0%
AAGCGCAGTGAAGGAGCCTT+TGG + chr5_4:43480707-43480726 Msa0852430:CDS 55.0%
! AAGAAGAATGGCGGGGACAG+AGG + chr5_4:43480680-43480699 Msa0852430:exon 55.0%
AAGCGCAGTGAAGGAGCCTT+TGG + chr5_4:43480707-43480726 Msa0852430:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 43480387 43481967 43480387 ID=Msa0852430;Name=Msa0852430
chr5_4 mRNA 43480387 43481967 43480387 ID=Msa0852430-mRNA-1;Parent=Msa0852430;Name=Msa0852430-mRNA-1;_AED=0.36;_eAED=0.36;_QI=161|1|1|1|0.66|0.5|4|442|98
chr5_4 exon 43480387 43480504 43480387 ID=Msa0852430-mRNA-1:exon:13739;Parent=Msa0852430-mRNA-1
chr5_4 exon 43480644 43480806 43480644 ID=Msa0852430-mRNA-1:exon:13740;Parent=Msa0852430-mRNA-1
chr5_4 exon 43480888 43480983 43480888 ID=Msa0852430-mRNA-1:exon:13741;Parent=Msa0852430-mRNA-1
chr5_4 exon 43481445 43481967 43481445 ID=Msa0852430-mRNA-1:exon:13742;Parent=Msa0852430-mRNA-1
chr5_4 five_prime_UTR 43480387 43480504 43480387 ID=Msa0852430-mRNA-1:five_prime_utr;Parent=Msa0852430-mRNA-1
chr5_4 five_prime_UTR 43480644 43480686 43480644 ID=Msa0852430-mRNA-1:five_prime_utr;Parent=Msa0852430-mRNA-1
chr5_4 CDS 43480687 43480806 43480687 ID=Msa0852430-mRNA-1:cds;Parent=Msa0852430-mRNA-1
chr5_4 CDS 43480888 43480983 43480888 ID=Msa0852430-mRNA-1:cds;Parent=Msa0852430-mRNA-1
chr5_4 CDS 43481445 43481525 43481445 ID=Msa0852430-mRNA-1:cds;Parent=Msa0852430-mRNA-1
chr5_4 three_prime_UTR 43481526 43481967 43481526 ID=Msa0852430-mRNA-1:three_prime_utr;Parent=Msa0852430-mRNA-1
Gene Sequence

>Msa0852430

ATGGCGGGGACAGAGGAAGAAAGCGCAGTGAAGGAGCCTTTGGATCTCATAAGGCTGAGTCTCGACGAACGTATCTACGTTAAACTCCGTTCTGATAGAGAGCTTCGTGGAAAACTTCATGCTTATGATCAACATCTTAATATGATTCTTGGTGATGTTGAAGAAATTGTTACTACTGTTGAAATTGATGATGAGACATATGAAGAAATTGTCAGGACTACAAAGCGGACAGTTCCGTTCCTTTTTGTTAGGGGAGATGGCGTGATATTGGTTTCTCCACCACTGAGGACTGCATAA
Protein sequence

>Msa0852430

MAGTEEESAVKEPLDLIRLSLDERIYVKLRSDRELRGKLHAYDQHLNMILGDVEEIVTTVEIDDETYEEIVRTTKRTVPFLFVRGDGVILVSPPLRTA*