AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0817570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817570 MtrunA17_Chr5g0436371 96.429 168 5 1 1 167 1 168 3.79e-116 330
Msa0817570 MtrunA17_Chr7g0258381 94.595 148 8 0 1 148 1 148 4.76e-94 269
Msa0817570 MtrunA17_Chr3g0113091 57.895 171 69 2 1 168 1 171 2.07e-60 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0817570 AT2G39920.4 47.436 156 79 2 1 153 1 156 1.97e-43 145
Msa0817570 AT2G39920.3 47.436 156 79 2 1 153 1 156 1.97e-43 145
Msa0817570 AT2G39920.1 47.436 156 79 2 1 153 1 156 1.97e-43 145
Msa0817570 AT2G39920.2 47.436 156 79 2 1 153 1 156 2.12e-43 145

Find 99 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATAACAAACAACATTATT+TGG 0.116637 5_3:-52899096 Msa0817570:five_prime_UTR
ATGTTGTGTTAGAATCTTAT+TGG 0.224960 5_3:-52899124 Msa0817570:five_prime_UTR
CTTAACATTATCTAGAATTA+AGG 0.225640 5_3:+52897593 None:intergenic
ACATGCTAAGCTCAATAATT+TGG 0.240869 5_3:-52898211 Msa0817570:CDS
AAGGAATGTGATACAGAAAA+AGG 0.244662 5_3:-52896795 Msa0817570:three_prime_UTR
TGTTTGTATATAAACTTAAA+TGG 0.251921 5_3:-52896314 Msa0817570:three_prime_UTR
TGCCGCAGTTCTAAGGGTTT+TGG 0.255038 5_3:-52896455 Msa0817570:three_prime_UTR
CCAAAGCTTAATCTATCTTT+AGG 0.258053 5_3:+52898877 None:intergenic
GTTGATTGATGGTGACATTT+TGG 0.278987 5_3:+52897228 None:intergenic
ATAGGTGCTGGATATATATT+AGG 0.288215 5_3:-52896645 Msa0817570:three_prime_UTR
AAATTACCTTAGTAACAAAT+TGG 0.311682 5_3:+52897996 None:intergenic
AATGAGGATTCCATTGAAAA+AGG 0.315752 5_3:+52898022 None:intergenic
GGTGTTCTGCCGCAGTTCTA+AGG 0.316307 5_3:-52896462 Msa0817570:three_prime_UTR
ATTGCTGGATTTGCTGCTCT+TGG 0.316792 5_3:-52898360 Msa0817570:CDS
TAAGTTTATATACAAACATT+AGG 0.318115 5_3:+52896319 None:intergenic
AGACAAATCGAACATTAATA+TGG 0.322829 5_3:+52897822 None:intergenic
CTTGTTGATGCTGGATTTAG+AGG 0.329191 5_3:-52897206 Msa0817570:three_prime_UTR
GACATAGACAAAGTGAATTA+TGG 0.338112 5_3:+52899252 None:intergenic
CTTTACTAATATTGTAAAAC+GGG 0.339757 5_3:+52897763 None:intergenic
AAATTTACAAATTATGTGTT+TGG 0.346031 5_3:+52896424 None:intergenic
CTTAGAAAATCACCGTAAAA+AGG 0.359497 5_3:+52896513 None:intergenic
GTAATACTCAATCACAGATT+TGG 0.359519 5_3:+52898104 None:intergenic
TCTGCAACAATCTTCATTGC+TGG 0.362031 5_3:-52898375 Msa0817570:CDS
AGAATTAAGGTTGTACATAA+TGG 0.364561 5_3:+52897606 None:intergenic
ATTCTTCCAATTTGTTACTA+AGG 0.365366 5_3:-52898002 Msa0817570:CDS
AGGAGTCTCTCAGCTGGTTC+TGG 0.366206 5_3:-52898809 Msa0817570:intron
TATAACAAACAACATTATTT+GGG 0.366501 5_3:-52899095 Msa0817570:intron
GAGGTGTGTTTGCGTATGTT+TGG 0.377332 5_3:-52896572 Msa0817570:three_prime_UTR
TAACTTTCAATTTGCATATG+TGG 0.384894 5_3:-52896286 Msa0817570:three_prime_UTR
ATTCTTGACTGCGAGTAATT+CGG 0.397431 5_3:-52897789 Msa0817570:three_prime_UTR
TGCAGTTAAGCCGGTGATTT+GGG 0.399324 5_3:+52899176 None:intergenic
TGCATATGTGGATATTGAAA+TGG 0.401677 5_3:-52896274 Msa0817570:three_prime_UTR
GTGCAGTTAAGCCGGTGATT+TGG 0.401813 5_3:+52899175 None:intergenic
GGCTCAAACATGTCACATAT+AGG 0.408550 5_3:+52896680 None:intergenic
TCTTGTATATTCTTAACATA+AGG 0.415938 5_3:+52897306 None:intergenic
ATCAATCAACTTGTTGATGC+TGG 0.420104 5_3:-52897215 Msa0817570:three_prime_UTR
AGCATCAACAAGTTGATTGA+TGG 0.423312 5_3:+52897217 None:intergenic
AGTGTTATTATGTCTGCTTA+TGG 0.427109 5_3:-52898854 Msa0817570:exon
GCTCAAACATGTCACATATA+GGG 0.427523 5_3:+52896681 None:intergenic
GCCAGGTCTTTACAAATGTT+AGG 0.429891 5_3:+52898174 None:intergenic
TAGACCAACCACTAGCTTGA+AGG 0.432274 5_3:+52897282 None:intergenic
ATATATAAATGCTAAGATCT+AGG 0.433644 5_3:-52897569 Msa0817570:three_prime_UTR
AGGTGTGTTTGCGTATGTTT+GGG 0.434144 5_3:-52896571 Msa0817570:three_prime_UTR
TCTTTACTAATATTGTAAAA+CGG 0.436017 5_3:+52897762 None:intergenic
CCTAAAGATAGATTAAGCTT+TGG 0.447877 5_3:-52898877 Msa0817570:five_prime_UTR
GTGTAGCTTCCACGTCAATC+AGG 0.447983 5_3:-52896483 Msa0817570:three_prime_UTR
TCATGTCAAAATAGCAATGC+TGG 0.451480 5_3:-52898291 Msa0817570:CDS
CTCGCAGTCAAGAATCTATA+CGG 0.456712 5_3:+52897797 None:intergenic
TTGATGCTGGATTTAGAGGC+TGG 0.460574 5_3:-52897202 Msa0817570:three_prime_UTR
TAAATATGAATCACAACTGC+GGG 0.462864 5_3:-52896165 Msa0817570:three_prime_UTR
ATGCTAAGATCTAGGACAGC+TGG 0.465364 5_3:-52897561 Msa0817570:three_prime_UTR
GTGATTGAGTATTACTTCAA+TGG 0.480480 5_3:-52898096 Msa0817570:CDS
AGTAACAAATTGGAAGAATG+AGG 0.486000 5_3:+52898006 None:intergenic
GGATTTGGATTTCATTTAGT+CGG 0.491559 5_3:-52897929 Msa0817570:three_prime_UTR
AACATGTCACATATAGGGTC+TGG 0.494617 5_3:+52896686 None:intergenic
AGTCACTCTGCTAGTTTCAA+TGG 0.498636 5_3:-52898328 Msa0817570:CDS
TCCCTACTTTCCCAAATCAC+CGG 0.500223 5_3:-52899186 Msa0817570:five_prime_UTR
GGTGTTAAACCATCAGAAGA+TGG 0.502777 5_3:-52898075 Msa0817570:CDS
GCCGGTGATTTGGGAAAGTA+GGG 0.503258 5_3:+52899185 None:intergenic
ATAATAATAATGTCTGTCCT+AGG 0.504381 5_3:-52899216 Msa0817570:five_prime_UTR
ATGCTGGAATTGTTGAGCTT+AGG 0.504865 5_3:-52898275 Msa0817570:CDS
GTGTTCTGCCGCAGTTCTAA+GGG 0.505357 5_3:-52896461 Msa0817570:three_prime_UTR
AGCCGGTGATTTGGGAAAGT+AGG 0.509707 5_3:+52899184 None:intergenic
TAAAATTTCAGGACACACCT+TGG 0.515008 5_3:-52898939 Msa0817570:intron
ATTAGTAAACTAATGCAAGT+TGG 0.523545 5_3:+52897510 None:intergenic
ACTGCGGGGTCAATTTCGTC+AGG 0.527374 5_3:-52896150 Msa0817570:three_prime_UTR
ACTGTTACAGATGCAGATAC+CGG 0.529547 5_3:-52896728 Msa0817570:three_prime_UTR
CTTTGATATATTGTAAAGCC+AGG 0.529642 5_3:+52898157 None:intergenic
TCCTAACATTTGTAAAGACC+TGG 0.529775 5_3:-52898175 Msa0817570:CDS
ATGTCACTGTCCCATGGCGG+TGG 0.553409 5_3:+52896537 None:intergenic
TCGGAGGAGAGCATAGGTGC+TGG 0.554025 5_3:-52896657 Msa0817570:three_prime_UTR
AGGTTGTACATAATGGATAG+AGG 0.558515 5_3:+52897613 None:intergenic
TATGTGACATGTTTGAGCCT+CGG 0.564759 5_3:-52896676 Msa0817570:three_prime_UTR
AAACTTAAAGTCGAGATGGC+CGG 0.565517 5_3:-52897905 Msa0817570:three_prime_UTR
TAAAACTATACATCATGAGC+CGG 0.566129 5_3:+52897886 None:intergenic
TCTACAAGGAGTCTCTCAGC+TGG 0.576969 5_3:-52898815 Msa0817570:CDS
TGGTCAATATGCAAGAGACT+TGG 0.581585 5_3:-52898133 Msa0817570:CDS
TTGTAGAATACATATGCTCA+TGG 0.586649 5_3:+52898831 None:intergenic
TACAAGAACCTTCAAGCTAG+TGG 0.590030 5_3:-52897290 Msa0817570:three_prime_UTR
ACATCAAAACCATCTTCTGA+TGG 0.590480 5_3:+52898066 None:intergenic
GCTGGTCTTCATTGCTGATG+AGG 0.591699 5_3:-52897184 Msa0817570:intron
CGGAAAACTTAAAGTCGAGA+TGG 0.602720 5_3:-52897909 Msa0817570:three_prime_UTR
AAAGGTATGTCACTGTCCCA+TGG 0.614415 5_3:+52896531 None:intergenic
TTGTTGGCAGTAACATGACA+AGG 0.616642 5_3:-52897404 Msa0817570:three_prime_UTR
CTGGCTTTACAATATATCAA+AGG 0.617801 5_3:-52898156 Msa0817570:CDS
CTAAATATGAATCACAACTG+CGG 0.621433 5_3:-52896166 Msa0817570:three_prime_UTR
AGAACCTTCAAGCTAGTGGT+TGG 0.622868 5_3:-52897286 Msa0817570:three_prime_UTR
TGAGCCTCGGAGGAGAGCAT+AGG 0.626848 5_3:-52896663 Msa0817570:three_prime_UTR
GCTTTACAATATATCAAAGG+TGG 0.628623 5_3:-52898153 Msa0817570:CDS
ATGAGCATATGTATTCTACA+AGG 0.656392 5_3:-52898829 Msa0817570:CDS
GTGACATGTTTGAGCCTCGG+AGG 0.664670 5_3:-52896673 Msa0817570:three_prime_UTR
AGCACCTATGCTCTCCTCCG+AGG 0.665355 5_3:+52896659 None:intergenic
TTTACTAATATTGTAAAACG+GGG 0.671258 5_3:+52897764 None:intergenic
AACTTGCTTCAAGAAATCCA+AGG 0.696146 5_3:+52898922 None:intergenic
AAAGTAGGGAAAGAGCACCT+AGG 0.697542 5_3:+52899199 None:intergenic
GTCCAAAACCCTTAGAACTG+CGG 0.710356 5_3:+52896453 None:intergenic
GGCAGAACACCTGATTGACG+TGG 0.718981 5_3:+52896474 None:intergenic
AAATATGAATCACAACTGCG+GGG 0.744274 5_3:-52896164 Msa0817570:three_prime_UTR
GGTATGTCACTGTCCCATGG+CGG 0.745152 5_3:+52896534 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTATCATTTAAGATT+TGG - chr5_3:52897416-52897435 Msa0817570:three_prime_UTR 10.0%
!! ATATAACAAACAACATTATT+TGG - chr5_3:52896299-52896318 Msa0817570:three_prime_UTR 15.0%
!! TATAACAAACAACATTATTT+GGG - chr5_3:52896300-52896319 Msa0817570:three_prime_UTR 15.0%
!!! CTAATTTTGATTAGTATTTT+TGG - chr5_3:52896924-52896943 Msa0817570:intron 15.0%
!!! ATTTCTATTAGTTTAGTTTT+TGG - chr5_3:52897444-52897463 Msa0817570:three_prime_UTR 15.0%
!!! TTTCTATTAGTTTAGTTTTT+GGG - chr5_3:52897445-52897464 Msa0817570:three_prime_UTR 15.0%
!! TCTTTACTAATATTGTAAAA+CGG + chr5_3:52897636-52897655 None:intergenic 15.0%
!!! ATTTGTTTTATAGATATCTA+AGG - chr5_3:52898260-52898279 Msa0817570:CDS 15.0%
!!! TTTTTTGTATATTAAGAAAG+AGG - chr5_3:52898804-52898823 Msa0817570:intron 15.0%
!! AAATTTACAAATTATGTGTT+TGG + chr5_3:52898974-52898993 None:intergenic 15.0%
!! TAAGTTTATATACAAACATT+AGG + chr5_3:52899079-52899098 None:intergenic 15.0%
!! TGTTTGTATATAAACTTAAA+TGG - chr5_3:52899081-52899100 Msa0817570:intron 15.0%
!! TAATTGCACTAAACAAATTT+AGG + chr5_3:52896350-52896369 None:intergenic 20.0%
!!! TATTTTTTCAATGAAACTGA+AGG - chr5_3:52896409-52896428 Msa0817570:three_prime_UTR 20.0%
!! AACAATTATTATCACCTATA+TGG - chr5_3:52896616-52896635 Msa0817570:three_prime_UTR 20.0%
!!! TTTTAGTCTTTGAAATTGTA+GGG - chr5_3:52896666-52896685 Msa0817570:three_prime_UTR 20.0%
!!! TTTTTTTTAGATCAGTTAGT+TGG + chr5_3:52896770-52896789 None:intergenic 20.0%
!! ATTTAGACCTTAAAACTATA+GGG + chr5_3:52896842-52896861 None:intergenic 20.0%
!! AATTTAGACCTTAAAACTAT+AGG + chr5_3:52896843-52896862 None:intergenic 20.0%
!! AAATTACCTTAGTAACAAAT+TGG + chr5_3:52897402-52897421 None:intergenic 20.0%
!! TTTACTAATATTGTAAAACG+GGG + chr5_3:52897634-52897653 None:intergenic 20.0%
!! CTTTACTAATATTGTAAAAC+GGG + chr5_3:52897635-52897654 None:intergenic 20.0%
!!! CTTAACATTATCTAGAATTA+AGG + chr5_3:52897805-52897824 None:intergenic 20.0%
!! ATATATAAATGCTAAGATCT+AGG - chr5_3:52897826-52897845 Msa0817570:three_prime_UTR 20.0%
!! TCTTGTATATTCTTAACATA+AGG + chr5_3:52898092-52898111 None:intergenic 20.0%
!!! AAAACAAATCTAGGATATAT+GGG + chr5_3:52898249-52898268 None:intergenic 20.0%
!!! TAAAACAAATCTAGGATATA+TGG + chr5_3:52898250-52898269 None:intergenic 20.0%
!! GATATCTATAAAACAAATCT+AGG + chr5_3:52898258-52898277 None:intergenic 20.0%
!! AGAAAAATATTCATTTGCTT+TGG + chr5_3:52898575-52898594 None:intergenic 20.0%
!!! ATGAATATTTTTCTAGACAA+AGG - chr5_3:52898581-52898600 Msa0817570:intron 20.0%
! ATAATAATAATGTCTGTCCT+AGG - chr5_3:52896179-52896198 Msa0817570:three_prime_UTR 25.0%
! ATGTTGTGTTAGAATCTTAT+TGG - chr5_3:52896271-52896290 Msa0817570:three_prime_UTR 25.0%
!!! GTTTTTGGTGTTATTTGAAT+TGG - chr5_3:52896493-52896512 Msa0817570:three_prime_UTR 25.0%
!!! GTTTTAGTCTTTGAAATTGT+AGG - chr5_3:52896665-52896684 Msa0817570:three_prime_UTR 25.0%
!!! TTTAGTCTTTGAAATTGTAG+GGG - chr5_3:52896667-52896686 Msa0817570:three_prime_UTR 25.0%
!!! AATTTCATTGATTTAGACGA+TGG + chr5_3:52896703-52896722 None:intergenic 25.0%
!! TTCATATGCAAAAGTCATTT+TGG - chr5_3:52896810-52896829 Msa0817570:three_prime_UTR 25.0%
!!! ATTCTTCCAATTTGTTACTA+AGG - chr5_3:52897393-52897412 Msa0817570:three_prime_UTR 25.0%
!!! TTAGTTTAGTTTTTGGGATT+TGG - chr5_3:52897451-52897470 Msa0817570:three_prime_UTR 25.0%
! AGACAAATCGAACATTAATA+TGG + chr5_3:52897576-52897595 None:intergenic 25.0%
! AGAATTAAGGTTGTACATAA+TGG + chr5_3:52897792-52897811 None:intergenic 25.0%
! ATTAGTAAACTAATGCAAGT+TGG + chr5_3:52897888-52897907 None:intergenic 25.0%
! TCTGCAAAAATAGAACATAT+TGG + chr5_3:52898533-52898552 None:intergenic 25.0%
! TAACTTTCAATTTGCATATG+TGG - chr5_3:52899109-52899128 Msa0817570:five_prime_UTR 25.0%
GACATAGACAAAGTGAATTA+TGG + chr5_3:52896146-52896165 None:intergenic 30.0%
TTGCACTAAACAAATTTAGG+TGG + chr5_3:52896347-52896366 None:intergenic 30.0%
TAGTGCAATTAATGTTGTCT+AGG - chr5_3:52896359-52896378 Msa0817570:three_prime_UTR 30.0%
!! CCTGAAATTTTAGAGAAACT+TGG + chr5_3:52896448-52896467 None:intergenic 30.0%
!! CCAAGTTTCTCTAAAATTTC+AGG - chr5_3:52896445-52896464 Msa0817570:three_prime_UTR 30.0%
! GATTTCTTGAAGCAAGTTTT+TGG - chr5_3:52896478-52896497 Msa0817570:three_prime_UTR 30.0%
CCAAAGCTTAATCTATCTTT+AGG + chr5_3:52896521-52896540 None:intergenic 30.0%
CCTAAAGATAGATTAAGCTT+TGG - chr5_3:52896518-52896537 Msa0817570:three_prime_UTR 30.0%
! AGTGTTATTATGTCTGCTTA+TGG - chr5_3:52896541-52896560 Msa0817570:three_prime_UTR 30.0%
TTGTAGAATACATATGCTCA+TGG + chr5_3:52896567-52896586 None:intergenic 30.0%
ATGAGCATATGTATTCTACA+AGG - chr5_3:52896566-52896585 Msa0817570:three_prime_UTR 30.0%
! TTCGATCATACTAGTCATTT+TGG - chr5_3:52896730-52896749 Msa0817570:three_prime_UTR 30.0%
TCTGTGAACAAGAAAAAAAC+AGG + chr5_3:52896951-52896970 None:intergenic 30.0%
!!! TTTTTTCTTGTTCACAGAGA+TGG - chr5_3:52896953-52896972 Msa0817570:intron 30.0%
! TTTTTCTTGTTCACAGAGAT+GGG - chr5_3:52896954-52896973 Msa0817570:intron 30.0%
ACATGCTAAGCTCAATAATT+TGG - chr5_3:52897184-52897203 Msa0817570:intron 30.0%
CTTTGATATATTGTAAAGCC+AGG + chr5_3:52897241-52897260 None:intergenic 30.0%
!! CTGGCTTTACAATATATCAA+AGG - chr5_3:52897239-52897258 Msa0817570:three_prime_UTR 30.0%
GCTTTACAATATATCAAAGG+TGG - chr5_3:52897242-52897261 Msa0817570:three_prime_UTR 30.0%
GTAATACTCAATCACAGATT+TGG + chr5_3:52897294-52897313 None:intergenic 30.0%
GTGATTGAGTATTACTTCAA+TGG - chr5_3:52897299-52897318 Msa0817570:three_prime_UTR 30.0%
!!! ACATAGATAGCCTTTTTCAA+TGG - chr5_3:52897363-52897382 Msa0817570:three_prime_UTR 30.0%
AATGAGGATTCCATTGAAAA+AGG + chr5_3:52897376-52897395 None:intergenic 30.0%
AGTAACAAATTGGAAGAATG+AGG + chr5_3:52897392-52897411 None:intergenic 30.0%
GGATTTGGATTTCATTTAGT+CGG - chr5_3:52897466-52897485 Msa0817570:three_prime_UTR 30.0%
TAAAACTATACATCATGAGC+CGG + chr5_3:52897512-52897531 None:intergenic 30.0%
! TGCTAATCTGCATATTTTGT+TGG - chr5_3:52897975-52897994 Msa0817570:three_prime_UTR 30.0%
! AACATGACAAGGTTTTCTTT+TGG - chr5_3:52898002-52898021 Msa0817570:CDS 30.0%
!! ATTTTGTTATCAAGAGAGTC+AGG - chr5_3:52898138-52898157 Msa0817570:CDS 30.0%
TCGATTACAAAGTACATGTT+TGG - chr5_3:52898352-52898371 Msa0817570:CDS 30.0%
AAAGTACATGTTTGGATTGA+CGG - chr5_3:52898360-52898379 Msa0817570:CDS 30.0%
TACATGTCACATATACTTTG+TGG + chr5_3:52898441-52898460 None:intergenic 30.0%
TCACATACTTTGTGTATGTT+TGG + chr5_3:52898493-52898512 None:intergenic 30.0%
! ACATATTGGTGCTTAAAATC+TGG + chr5_3:52898519-52898538 None:intergenic 30.0%
AAGGAATGTGATACAGAAAA+AGG - chr5_3:52898600-52898619 Msa0817570:intron 30.0%
AGGAATGTGATACAGAAAAA+GGG - chr5_3:52898601-52898620 Msa0817570:intron 30.0%
!! ATAGGTGCTGGATATATATT+AGG - chr5_3:52898750-52898769 Msa0817570:intron 30.0%
!! AGGTTTTATCCTAGAGTTTT+TGG - chr5_3:52898770-52898789 Msa0817570:intron 30.0%
TTTATCGAACCAAAAACTCT+AGG + chr5_3:52898782-52898801 None:intergenic 30.0%
CTTAGAAAATCACCGTAAAA+AGG + chr5_3:52898885-52898904 None:intergenic 30.0%
TGCATATGTGGATATTGAAA+TGG - chr5_3:52899121-52899140 Msa0817570:five_prime_UTR 30.0%
AACACATGCGTGAAAAAATA+TGG - chr5_3:52899179-52899198 Msa0817570:intron 30.0%
CTAAATATGAATCACAACTG+CGG - chr5_3:52899229-52899248 Msa0817570:five_prime_UTR 30.0%
TAAATATGAATCACAACTGC+GGG - chr5_3:52899230-52899249 Msa0817570:five_prime_UTR 30.0%
TTTAGGTGGAAGAAAAAGAG+TGG + chr5_3:52896333-52896352 None:intergenic 35.0%
TAAAATTTCAGGACACACCT+TGG - chr5_3:52896456-52896475 Msa0817570:three_prime_UTR 35.0%
! AACTTGCTTCAAGAAATCCA+AGG + chr5_3:52896476-52896495 None:intergenic 35.0%
ATGAGCAGAATCTACCATAT+AGG + chr5_3:52896633-52896652 None:intergenic 35.0%
! TTTTCTTGTTCACAGAGATG+GGG - chr5_3:52896955-52896974 Msa0817570:intron 35.0%
!!! AGTAGTTTTGTGCTTGAATC+AGG - chr5_3:52896978-52896997 Msa0817570:intron 35.0%
TCATGTCAAAATAGCAATGC+TGG - chr5_3:52897104-52897123 Msa0817570:intron 35.0%
TCCTAACATTTGTAAAGACC+TGG - chr5_3:52897220-52897239 Msa0817570:three_prime_UTR 35.0%
ACATCAAAACCATCTTCTGA+TGG + chr5_3:52897332-52897351 None:intergenic 35.0%
!!! ATCAGAAGATGGTTTTGATG+TGG - chr5_3:52897331-52897350 Msa0817570:three_prime_UTR 35.0%
ATTCTTGACTGCGAGTAATT+CGG - chr5_3:52897606-52897625 Msa0817570:three_prime_UTR 35.0%
!! AGGTTGTACATAATGGATAG+AGG + chr5_3:52897785-52897804 None:intergenic 35.0%
!! ACAAGGTTTTCTTTTGGTGT+TGG - chr5_3:52898008-52898027 Msa0817570:CDS 35.0%
!!! GTTGATTGATGGTGACATTT+TGG + chr5_3:52898170-52898189 None:intergenic 35.0%
!! AGCATCAACAAGTTGATTGA+TGG + chr5_3:52898181-52898200 None:intergenic 35.0%
!! ATCAATCAACTTGTTGATGC+TGG - chr5_3:52898180-52898199 Msa0817570:CDS 35.0%
! ACAAATCTAGGATATATGGG+TGG + chr5_3:52898246-52898265 None:intergenic 35.0%
CAATGAGTTTGGCAAAATCA+CGG + chr5_3:52898413-52898432 None:intergenic 35.0%
TTGTGGATGAACAATGAGTT+TGG + chr5_3:52898424-52898443 None:intergenic 35.0%
! TTTTGCAGAACAGAAGATGA+AGG - chr5_3:52898544-52898563 Msa0817570:intron 35.0%
TGATACAGAAAAAGGGTTTC+AGG - chr5_3:52898608-52898627 Msa0817570:intron 35.0%
TAGCAAAAACTTTCGCATTC+CGG + chr5_3:52898689-52898708 None:intergenic 35.0%
GCTCAAACATGTCACATATA+GGG + chr5_3:52898717-52898736 None:intergenic 35.0%
! GACAGTGACATACCTTTTTA+CGG - chr5_3:52898870-52898889 Msa0817570:five_prime_UTR 35.0%
!!! TTTTTTCACGCATGTGTTCT+TGG + chr5_3:52899177-52899196 None:intergenic 35.0%
AAATATGAATCACAACTGCG+GGG - chr5_3:52899231-52899250 Msa0817570:five_prime_UTR 35.0%
AGACCTTAAAACTATAGGGC+AGG + chr5_3:52896838-52896857 None:intergenic 40.0%
TCTGCAACAATCTTCATTGC+TGG - chr5_3:52897020-52897039 Msa0817570:intron 40.0%
! AGTCACTCTGCTAGTTTCAA+TGG - chr5_3:52897067-52897086 Msa0817570:intron 40.0%
ATGCTGGAATTGTTGAGCTT+AGG - chr5_3:52897120-52897139 Msa0817570:intron 40.0%
GCCAGGTCTTTACAAATGTT+AGG + chr5_3:52897224-52897243 None:intergenic 40.0%
TGGTCAATATGCAAGAGACT+TGG - chr5_3:52897262-52897281 Msa0817570:three_prime_UTR 40.0%
GGTGTTAAACCATCAGAAGA+TGG - chr5_3:52897320-52897339 Msa0817570:three_prime_UTR 40.0%
CGGAAAACTTAAAGTCGAGA+TGG - chr5_3:52897486-52897505 Msa0817570:three_prime_UTR 40.0%
AAACTTAAAGTCGAGATGGC+CGG - chr5_3:52897490-52897509 Msa0817570:three_prime_UTR 40.0%
CTCGCAGTCAAGAATCTATA+CGG + chr5_3:52897601-52897620 None:intergenic 40.0%
TTGTTGGCAGTAACATGACA+AGG - chr5_3:52897991-52898010 Msa0817570:exon 40.0%
TACAAGAACCTTCAAGCTAG+TGG - chr5_3:52898105-52898124 Msa0817570:CDS 40.0%
CTTGTTGATGCTGGATTTAG+AGG - chr5_3:52898189-52898208 Msa0817570:CDS 40.0%
ACTGTTACAGATGCAGATAC+CGG - chr5_3:52898667-52898686 Msa0817570:intron 40.0%
AACATGTCACATATAGGGTC+TGG + chr5_3:52898712-52898731 None:intergenic 40.0%
GGCTCAAACATGTCACATAT+AGG + chr5_3:52898718-52898737 None:intergenic 40.0%
TATGTGACATGTTTGAGCCT+CGG - chr5_3:52898719-52898738 Msa0817570:intron 40.0%
AGGTGTGTTTGCGTATGTTT+GGG - chr5_3:52898824-52898843 Msa0817570:CDS 40.0%
!! AAAGTAGGGAAAGAGCACCT+AGG + chr5_3:52896199-52896218 None:intergenic 45.0%
TCCCTACTTTCCCAAATCAC+CGG - chr5_3:52896209-52896228 Msa0817570:three_prime_UTR 45.0%
TGCAGTTAAGCCGGTGATTT+GGG + chr5_3:52896222-52896241 None:intergenic 45.0%
GAACATGTGTGCAGTTAAGC+CGG + chr5_3:52896231-52896250 None:intergenic 45.0%
CTGCACACATGTTCACAACA+TGG - chr5_3:52896237-52896256 Msa0817570:three_prime_UTR 45.0%
!! CTTTGAAATTGTAGGGGACG+AGG - chr5_3:52896673-52896692 Msa0817570:three_prime_UTR 45.0%
!! GAGCCTGCCCTATAGTTTTA+AGG - chr5_3:52896832-52896851 Msa0817570:three_prime_UTR 45.0%
!! ATTGCTGGATTTGCTGCTCT+TGG - chr5_3:52897035-52897054 Msa0817570:intron 45.0%
! ATGCTAAGATCTAGGACAGC+TGG - chr5_3:52897834-52897853 Msa0817570:three_prime_UTR 45.0%
AGAACCTTCAAGCTAGTGGT+TGG - chr5_3:52898109-52898128 Msa0817570:CDS 45.0%
! TAGACCAACCACTAGCTTGA+AGG + chr5_3:52898116-52898135 None:intergenic 45.0%
TTGATGCTGGATTTAGAGGC+TGG - chr5_3:52898193-52898212 Msa0817570:CDS 45.0%
TGAGTTTGGCAAAATCACGG+TGG + chr5_3:52898410-52898429 None:intergenic 45.0%
GAGGTGTGTTTGCGTATGTT+TGG - chr5_3:52898823-52898842 Msa0817570:CDS 45.0%
! AAAGGTATGTCACTGTCCCA+TGG + chr5_3:52898867-52898886 None:intergenic 45.0%
GTCCAAAACCCTTAGAACTG+CGG + chr5_3:52898945-52898964 None:intergenic 45.0%
!!! AGTTCTAAGGGTTTTGGACG+CGG - chr5_3:52898946-52898965 Msa0817570:intron 45.0%
! GCCGGTGATTTGGGAAAGTA+GGG + chr5_3:52896213-52896232 None:intergenic 50.0%
! AGCCGGTGATTTGGGAAAGT+AGG + chr5_3:52896214-52896233 None:intergenic 50.0%
GTGCAGTTAAGCCGGTGATT+TGG + chr5_3:52896223-52896242 None:intergenic 50.0%
TCTACAAGGAGTCTCTCAGC+TGG - chr5_3:52896580-52896599 Msa0817570:three_prime_UTR 50.0%
!! GCTGGTCTTCATTGCTGATG+AGG - chr5_3:52898211-52898230 Msa0817570:CDS 50.0%
GTGTAGCTTCCACGTCAATC+AGG - chr5_3:52898912-52898931 Msa0817570:five_prime_UTR 50.0%
! GTGTTCTGCCGCAGTTCTAA+GGG - chr5_3:52898934-52898953 Msa0817570:intron 50.0%
!!! TGCCGCAGTTCTAAGGGTTT+TGG - chr5_3:52898940-52898959 Msa0817570:intron 50.0%
! AGGAGTCTCTCAGCTGGTTC+TGG - chr5_3:52896586-52896605 Msa0817570:three_prime_UTR 55.0%
GTGACATGTTTGAGCCTCGG+AGG - chr5_3:52898722-52898741 Msa0817570:intron 55.0%
GGTATGTCACTGTCCCATGG+CGG + chr5_3:52898864-52898883 None:intergenic 55.0%
GGCAGAACACCTGATTGACG+TGG + chr5_3:52898924-52898943 None:intergenic 55.0%
GGTGTTCTGCCGCAGTTCTA+AGG - chr5_3:52898933-52898952 Msa0817570:intron 55.0%
ACTGCGGGGTCAATTTCGTC+AGG - chr5_3:52899245-52899264 Msa0817570:five_prime_UTR 55.0%
! TGAGCCTCGGAGGAGAGCAT+AGG - chr5_3:52898732-52898751 Msa0817570:intron 60.0%
AGCACCTATGCTCTCCTCCG+AGG + chr5_3:52898739-52898758 None:intergenic 60.0%
! TCGGAGGAGAGCATAGGTGC+TGG - chr5_3:52898738-52898757 Msa0817570:intron 60.0%
! TGAGCGTTTTGCCACCGCCA+TGG - chr5_3:52898847-52898866 Msa0817570:CDS 60.0%
! GAGCGTTTTGCCACCGCCAT+GGG - chr5_3:52898848-52898867 Msa0817570:CDS 60.0%
ATGTCACTGTCCCATGGCGG+TGG + chr5_3:52898861-52898880 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 52896143 52899274 52896143 ID=Msa0817570;Name=Msa0817570
chr5_3 mRNA 52896143 52899274 52896143 ID=Msa0817570-mRNA-1;Parent=Msa0817570;Name=Msa0817570-mRNA-1;_AED=0.32;_eAED=0.32;_QI=232|1|1|1|0.5|0.2|5|1537|168
chr5_3 exon 52896143 52896865 52896143 ID=Msa0817570-mRNA-1:exon:15967;Parent=Msa0817570-mRNA-1
chr5_3 exon 52897185 52898447 52897185 ID=Msa0817570-mRNA-1:exon:15966;Parent=Msa0817570-mRNA-1
chr5_3 exon 52898810 52898950 52898810 ID=Msa0817570-mRNA-1:exon:15965;Parent=Msa0817570-mRNA-1
chr5_3 exon 52899096 52899154 52899096 ID=Msa0817570-mRNA-1:exon:15964;Parent=Msa0817570-mRNA-1
chr5_3 exon 52899185 52899274 52899185 ID=Msa0817570-mRNA-1:exon:15963;Parent=Msa0817570-mRNA-1
chr5_3 five_prime_UTR 52899185 52899274 52899185 ID=Msa0817570-mRNA-1:five_prime_utr;Parent=Msa0817570-mRNA-1
chr5_3 five_prime_UTR 52899096 52899154 52899096 ID=Msa0817570-mRNA-1:five_prime_utr;Parent=Msa0817570-mRNA-1
chr5_3 five_prime_UTR 52898868 52898950 52898868 ID=Msa0817570-mRNA-1:five_prime_utr;Parent=Msa0817570-mRNA-1
chr5_3 CDS 52898810 52898867 52898810 ID=Msa0817570-mRNA-1:cds;Parent=Msa0817570-mRNA-1
chr5_3 CDS 52897999 52898447 52897999 ID=Msa0817570-mRNA-1:cds;Parent=Msa0817570-mRNA-1
chr5_3 three_prime_UTR 52897185 52897998 52897185 ID=Msa0817570-mRNA-1:three_prime_utr;Parent=Msa0817570-mRNA-1
chr5_3 three_prime_UTR 52896143 52896865 52896143 ID=Msa0817570-mRNA-1:three_prime_utr;Parent=Msa0817570-mRNA-1
Gene Sequence

>Msa0817570

ATGTCTGCTTATGGCCATGAGCATATGTATTCTACAAGGAGTCTCTCAGCTGGTTCTGAGATGGGGAGTAGTTTTGTGCTTGAATCAGGTTTCTACATCACATCATTTTCTGCAACAATCTTCATTGCTGGATTTGCTGCTCTTGGACTTTTACTAGTCACTCTGCTAGTTTCAATGGCAATGATGCTACAATCATGTCAAAATAGCAATGCTGGAATTGTTGAGCTTAGGAATATAAATGATGACTATAGTTATTGCAAAATTCATTCTCTACATGCTAAGCTCAATAATTTGGAAGAATATAATGTTCCTAACATTTGTAAAGACCTGGCTTTACAATATATCAAAGGTGGTCAATATGCAAGAGACTTGGATTCAACCAAATCTGTGATTGAGTATTACTTCAATGGTGTTAAACCATCAGAAGATGGTTTTGATGTGGTGTTGATAGACATAGATAGCCTTTTTCAATGGAATCCTCATTCTTCCAATTTGTTACTAAGGTAA
Protein sequence

>Msa0817570

MSAYGHEHMYSTRSLSAGSEMGSSFVLESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNAGIVELRNINDDYSYCKIHSLHAKLNNLEEYNVPNICKDLALQYIKGGQYARDLDSTKSVIEYYFNGVKPSEDGFDVVLIDIDSLFQWNPHSSNLLLR*