AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0738130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0738130 MtrunA17_Chr5g0436371 94.276 297 16 1 1 296 1 297 0.0 552
Msa0738130 MtrunA17_Chr3g0113091 59.468 301 112 4 1 293 1 299 3.35e-119 344
Msa0738130 MtrunA17_Chr7g0258381 93.243 148 10 0 1 148 1 148 9.14e-91 266
Msa0738130 MtrunA17_Chr7g0258401 88.660 97 11 0 184 280 1 97 1.11e-57 180
Msa0738130 MtrunA17_Chr8g0356021 28.302 212 138 5 86 286 47 255 1.90e-18 82.8
Msa0738130 MtrunA17_Chr1g0186301 25.379 264 171 8 41 286 3 258 3.69e-17 79.3
Msa0738130 MtrunA17_Chr6g0487381 26.761 213 144 4 84 286 38 248 3.51e-15 73.6
Msa0738130 MtrunA17_Chr1g0168451 24.498 249 163 8 51 286 26 262 9.04e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0738130 AT2G39920.4 39.446 289 150 6 1 283 1 270 6.64e-62 197
Msa0738130 AT2G39920.3 39.446 289 150 6 1 283 1 270 6.64e-62 197
Msa0738130 AT2G39920.1 39.446 289 150 6 1 283 1 270 6.64e-62 197
Msa0738130 AT2G39920.2 39.100 289 150 6 1 283 1 269 1.90e-61 196
Msa0738130 AT4G29270.1 32.710 214 130 6 84 286 42 252 7.93e-24 97.8
Msa0738130 AT4G25150.1 25.379 264 164 8 48 286 1 256 3.53e-17 79.7
Msa0738130 AT4G29260.1 28.054 221 139 5 80 286 37 251 1.44e-16 78.2
Msa0738130 AT5G51260.1 25.926 189 120 4 85 258 44 227 1.25e-14 72.8
Msa0738130 AT5G44020.1 24.454 229 156 7 72 286 43 268 9.99e-13 67.4
Msa0738130 AT1G04040.1 23.176 233 163 6 68 286 37 267 2.06e-11 63.5

Find 83 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTTGGATTTCAAATTT+TGG 0.145660 5_1:+55825645 None:intergenic
CCAAGTTTCTCTAAAATTTC+AGG 0.148674 5_1:-55828376 Msa0738130:five_prime_UTR
AGCTTGTCTCTTAACGAAAT+TGG 0.198410 5_1:+55825551 None:intergenic
ACATGCTAAGCTCAATAATT+TGG 0.229680 5_1:-55827638 Msa0738130:CDS
AAGGAATGTGATACAGAAAA+AGG 0.244662 5_1:-55826205 Msa0738130:CDS
TGTTTGTATATAAACTTAAA+TGG 0.251921 5_1:-55825724 Msa0738130:three_prime_UTR
TGCCCCAGTTCTAAGGGTTT+TGG 0.255038 5_1:-55825865 Msa0738130:three_prime_UTR
CCAAAGCTTAATCTATCTTT+AGG 0.258053 5_1:+55828303 None:intergenic
GTTGATTGATGGTGACATTT+TGG 0.278987 5_1:+55826655 None:intergenic
AACACATGCATGACAAAATA+TGG 0.282125 5_1:-55825626 Msa0738130:three_prime_UTR
ATAGGTGCTGGATATATATT+AGG 0.288215 5_1:-55826055 Msa0738130:exon
CATGAGCAAGCCGGTGATTT+GGG 0.305766 5_1:+55828570 None:intergenic
AAATTACCTTAGTAACAAAT+TGG 0.311682 5_1:+55827423 None:intergenic
AATGAGGATTCCATTGAAAA+AGG 0.315752 5_1:+55827449 None:intergenic
ATTGCTGGATTTGCTGCTCT+TGG 0.316792 5_1:-55827787 Msa0738130:CDS
TAAGTTTATATACAAACATT+AGG 0.318115 5_1:+55825729 None:intergenic
ACATGAGCAAGCCGGTGATT+TGG 0.318554 5_1:+55828569 None:intergenic
CTTGTTGATGCTGGATTTAG+AGG 0.329191 5_1:-55826633 Msa0738130:CDS
GGTGTTCTGCCCCAGTTCTA+AGG 0.329796 5_1:-55825872 Msa0738130:three_prime_UTR
AAATTTACAAATTATGTGTT+TGG 0.346031 5_1:+55825834 None:intergenic
CTTAGAAAATCACCGTAAAA+AGG 0.359497 5_1:+55825923 None:intergenic
GTAATACTCAATCACAGATT+TGG 0.359519 5_1:+55827531 None:intergenic
TCTGCAACAATCTTCATTGC+TGG 0.362031 5_1:-55827802 Msa0738130:CDS
ATTCTTCCAATTTGTTACTA+AGG 0.365366 5_1:-55827429 Msa0738130:intron
AGGAGTCTCTCAGCTGGTTC+TGG 0.366206 5_1:-55828235 Msa0738130:intron
GAGGTGTGTTTGCGTATGTT+TGG 0.377332 5_1:-55825982 Msa0738130:three_prime_UTR
TAACTTTCAATTTGCATATG+TGG 0.384894 5_1:-55825696 Msa0738130:three_prime_UTR
TGCATATGTGGATATTGAAA+TGG 0.401677 5_1:-55825684 Msa0738130:three_prime_UTR
GCGTCCAAAACCCTTAGAAC+TGG 0.407978 5_1:+55825861 None:intergenic
GGCTCAAACATGTCACATAT+AGG 0.417441 5_1:+55826090 None:intergenic
ATCAATCAACTTGTTGATGC+TGG 0.420104 5_1:-55826642 Msa0738130:CDS
AGCATCAACAAGTTGATTGA+TGG 0.423312 5_1:+55826644 None:intergenic
GACAGGTCTTTACAAATGTT+AGG 0.426632 5_1:+55827601 None:intergenic
AGTGTTATTATGTCTGCTTA+TGG 0.427109 5_1:-55828280 Msa0738130:exon
GCTCAAACATGTCACATATA+GGG 0.427523 5_1:+55826091 None:intergenic
TAGACCAACCACTAGCTTGA+AGG 0.432274 5_1:+55826709 None:intergenic
AGGTGTGTTTGCGTATGTTT+GGG 0.434144 5_1:-55825981 Msa0738130:three_prime_UTR
AGTCACTCTGCTAGTTTCAA+TGG 0.436671 5_1:-55827755 Msa0738130:CDS
CCTAAAGATAGATTAAGCTT+TGG 0.447877 5_1:-55828303 Msa0738130:five_prime_UTR
GTGTAGCTTCCACGTCAATC+AGG 0.447983 5_1:-55825893 Msa0738130:three_prime_UTR
TCATGTCAAAACAGCAATGC+TGG 0.450576 5_1:-55827718 Msa0738130:CDS
GCTGGTCTTCATTGCTGATA+AGG 0.453762 5_1:-55826611 Msa0738130:intron
TTGATGCTGGATTTAGAGGC+TGG 0.460574 5_1:-55826629 Msa0738130:CDS
GTGTTCTGCCCCAGTTCTAA+GGG 0.472649 5_1:-55825871 Msa0738130:three_prime_UTR
TAAATCTGAATCACAACTGC+GGG 0.479847 5_1:-55825575 Msa0738130:three_prime_UTR
GTGATTGAGTATTACTTCAA+TGG 0.480480 5_1:-55827523 Msa0738130:CDS
AGTAACAAATTGGAAGAATG+AGG 0.486000 5_1:+55827433 None:intergenic
AACATGTCACATATAGGGTC+TGG 0.494617 5_1:+55826096 None:intergenic
TCCCTACCTTCCCAAATCAC+CGG 0.500223 5_1:-55828580 Msa0738130:five_prime_UTR
GGTGTTAAACCATCAGAAGA+TGG 0.502777 5_1:-55827502 Msa0738130:CDS
CGTCCAAAACCCTTAGAACT+GGG 0.504051 5_1:+55825862 None:intergenic
ATAATAATAATGTCTGTCCT+AGG 0.504381 5_1:-55828610 Msa0738130:five_prime_UTR
ATGCTGGAATTATTGAGCTT+AGG 0.508522 5_1:-55827702 Msa0738130:CDS
ACTGTTACACATGCAGATAC+CGG 0.511047 5_1:-55826138 Msa0738130:CDS
TAAAATTTCAGGACACACCT+TGG 0.515008 5_1:-55828365 Msa0738130:five_prime_UTR
CTTTGATATATTGTAAAGAC+AGG 0.522975 5_1:+55827584 None:intergenic
TCAGAGGAGAGCATAGGTGC+TGG 0.524135 5_1:-55826067 Msa0738130:exon
GCCGGTGATTTGGGAAGGTA+GGG 0.527392 5_1:+55828579 None:intergenic
TTTATCGAACCAAATACTCT+AGG 0.552924 5_1:+55826026 None:intergenic
ATGTCACTGTCCCATGGCGG+TGG 0.553409 5_1:+55825947 None:intergenic
AGCCGGTGATTTGGGAAGGT+AGG 0.576640 5_1:+55828578 None:intergenic
TCTACAAGGAGTCTCTCAGC+TGG 0.576969 5_1:-55828241 Msa0738130:CDS
TGGTCAATATGCAAGAGACT+TGG 0.581585 5_1:-55827560 Msa0738130:CDS
TTGTAGAATACATATGCTCA+TGG 0.586649 5_1:+55828257 None:intergenic
TACAAGAACCTTCAAGCTAG+TGG 0.590030 5_1:-55826717 Msa0738130:CDS
ACATCAAAACCATCTTCTGA+TGG 0.590480 5_1:+55827493 None:intergenic
AGCAAGCCGGTGATTTGGGA+AGG 0.599055 5_1:+55828574 None:intergenic
AAAGGTATGTCACTGTCCCA+TGG 0.614415 5_1:+55825941 None:intergenic
TGAGCCTCAGAGGAGAGCAT+AGG 0.617171 5_1:-55826073 Msa0738130:exon
AGCACCTATGCTCTCCTCTG+AGG 0.619362 5_1:+55826069 None:intergenic
AGAACCTTCAAGCTAGTGGT+TGG 0.622868 5_1:-55826713 Msa0738130:CDS
AACTTGCTTCAACAAATCCA+AGG 0.627973 5_1:+55828348 None:intergenic
TCTTTACAATATATCAAAGG+TGG 0.628623 5_1:-55827580 Msa0738130:CDS
GTGACATGTTTGAGCCTCAG+AGG 0.633336 5_1:-55826083 Msa0738130:CDS
CTGTCTTTACAATATATCAA+AGG 0.633916 5_1:-55827583 Msa0738130:CDS
ATGAGCATATGTATTCTACA+AGG 0.656392 5_1:-55828255 Msa0738130:CDS
CTAACACAACATGAGCAAGC+CGG 0.666323 5_1:+55828561 None:intergenic
GGCAGAACACCTGATTGACG+TGG 0.671532 5_1:+55825884 None:intergenic
CTAAATCTGAATCACAACTG+CGG 0.672658 5_1:-55825576 Msa0738130:three_prime_UTR
AAGGTAGGGAAAGAGCACCT+AGG 0.697542 5_1:+55828593 None:intergenic
GTCCAAAACCCTTAGAACTG+GGG 0.707945 5_1:+55825863 None:intergenic
GGTATGTCACTGTCCCATGG+CGG 0.745152 5_1:+55825944 None:intergenic
AAATCTGAATCACAACTGCG+GGG 0.748308 5_1:-55825574 Msa0738130:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTATCATTTAAGATT+TGG - chr5_1:55826773-55826792 Msa0738130:CDS 10.0%
!! ATATAACAAACAACATTATT+TGG - chr5_1:55825657-55825676 Msa0738130:three_prime_UTR 15.0%
!! TATAACAAACAACATTATTT+GGG - chr5_1:55825658-55825677 Msa0738130:three_prime_UTR 15.0%
!!! CTAATTTTGATTAGTATTTT+TGG - chr5_1:55826281-55826300 Msa0738130:intron 15.0%
!!! ATTTCTATTAGTTTAGTTTT+TGG - chr5_1:55826801-55826820 Msa0738130:intron 15.0%
!!! TTTCTATTAGTTTAGTTTTT+GGG - chr5_1:55826802-55826821 Msa0738130:intron 15.0%
!! TCTTTACTAATATTGTAAAA+CGG + chr5_1:55826993-55827012 None:intergenic 15.0%
!!! TTTAACATTATCTAGAATTA+AGG + chr5_1:55827162-55827181 None:intergenic 15.0%
!!! TTTTTTGTATATTAAGAAAG+AGG - chr5_1:55828178-55828197 Msa0738130:intron 15.0%
!! AAATTTACAAATTATGTGTT+TGG + chr5_1:55828348-55828367 None:intergenic 15.0%
!! TAAGTTTATATACAAACATT+AGG + chr5_1:55828453-55828472 None:intergenic 15.0%
!! TGTTTGTATATAAACTTAAA+TGG - chr5_1:55828455-55828474 Msa0738130:intron 15.0%
!! TAATTGCACTAAACAAATTT+AGG + chr5_1:55825708-55825727 None:intergenic 20.0%
!!! TATTTTTTCAATGAAACTGA+AGG - chr5_1:55825767-55825786 Msa0738130:three_prime_UTR 20.0%
!!! AATATCATTGATTTAGACTA+TGG + chr5_1:55826059-55826078 None:intergenic 20.0%
!!! TTTTTTTTAGATCAGTTAGT+CGG + chr5_1:55826126-55826145 None:intergenic 20.0%
!!! TTCATATTCAAAAGTCATTT+TGG - chr5_1:55826166-55826185 Msa0738130:CDS 20.0%
!! ATTTAGTCCTTAAAACTATA+GGG + chr5_1:55826199-55826218 None:intergenic 20.0%
!! AATTTAGTCCTTAAAACTAT+AGG + chr5_1:55826200-55826219 None:intergenic 20.0%
!! AAATTACCTTAGTAACAAAT+TGG + chr5_1:55826759-55826778 None:intergenic 20.0%
!! TTTACTAATATTGTAAAACG+GGG + chr5_1:55826991-55827010 None:intergenic 20.0%
!! CTTTACTAATATTGTAAAAC+GGG + chr5_1:55826992-55827011 None:intergenic 20.0%
!! TATCTATAAAACAACATTTG+TGG + chr5_1:55827638-55827657 None:intergenic 20.0%
!!! TGTTGTTTTATAGATATCTA+AGG - chr5_1:55827642-55827661 Msa0738130:CDS 20.0%
!! AGAAAAATATTCATTTGCTT+TGG + chr5_1:55827949-55827968 None:intergenic 20.0%
!!! ATGAATATTTTTCTAGACAA+AGG - chr5_1:55827955-55827974 Msa0738130:intron 20.0%
! ATAATAATAATGTCTGTCCT+AGG - chr5_1:55825569-55825588 Msa0738130:three_prime_UTR 25.0%
! ATGTTGTGTTAGAATCTTAT+TGG - chr5_1:55825629-55825648 Msa0738130:three_prime_UTR 25.0%
! TGTTGTGTTAGAATCTTATT+GGG - chr5_1:55825630-55825649 Msa0738130:three_prime_UTR 25.0%
!!! GTTTTTGGTGTTATTTGAAT+TGG - chr5_1:55825851-55825870 Msa0738130:three_prime_UTR 25.0%
!! TTTTAGTCCTTGAAATTGTA+GGG - chr5_1:55826022-55826041 Msa0738130:three_prime_UTR 25.0%
! CTTTGATATATTGTAAAGAC+AGG + chr5_1:55826598-55826617 None:intergenic 25.0%
! CTGTCTTTACAATATATCAA+AGG - chr5_1:55826596-55826615 Msa0738130:intron 25.0%
! TCTTTACAATATATCAAAGG+TGG - chr5_1:55826599-55826618 Msa0738130:intron 25.0%
!!! ATTCTTCCAATTTGTTACTA+AGG - chr5_1:55826750-55826769 Msa0738130:CDS 25.0%
!!! TTAGTTTAGTTTTTGGGATT+TGG - chr5_1:55826808-55826827 Msa0738130:intron 25.0%
! AGACAAATCGAACATTAATA+TGG + chr5_1:55826933-55826952 None:intergenic 25.0%
! AGAATTAAGGTTGTACATAA+TGG + chr5_1:55827149-55827168 None:intergenic 25.0%
! ATTAGTAAACTAATGCAAGT+TGG + chr5_1:55827245-55827264 None:intergenic 25.0%
!! AACCATTATCTAGGATATAT+GGG + chr5_1:55827606-55827625 None:intergenic 25.0%
! TCTGCAAAAATAGAACATAT+TGG + chr5_1:55827907-55827926 None:intergenic 25.0%
! TAACTTTCAATTTGCATATG+TGG - chr5_1:55828483-55828502 Msa0738130:intron 25.0%
!! TGTTCTTGGATTTCAAATTT+TGG + chr5_1:55828537-55828556 None:intergenic 25.0%
TTGCACTAAACAAATTTAGG+TGG + chr5_1:55825705-55825724 None:intergenic 30.0%
TAGTGCAATTAATGTTGTCT+AGG - chr5_1:55825717-55825736 Msa0738130:three_prime_UTR 30.0%
!! CCTGAAATTTTAGAGAAACT+TGG + chr5_1:55825806-55825825 None:intergenic 30.0%
!! CCAAGTTTCTCTAAAATTTC+AGG - chr5_1:55825803-55825822 Msa0738130:three_prime_UTR 30.0%
!!! GATTTGTTGAAGCAAGTTTT+TGG - chr5_1:55825836-55825855 Msa0738130:three_prime_UTR 30.0%
CCAAAGCTTAATCTATCTTT+AGG + chr5_1:55825879-55825898 None:intergenic 30.0%
CCTAAAGATAGATTAAGCTT+TGG - chr5_1:55825876-55825895 Msa0738130:three_prime_UTR 30.0%
! AGTGTTATTATGTCTGCTTA+TGG - chr5_1:55825899-55825918 Msa0738130:three_prime_UTR 30.0%
TTGTAGAATACATATGCTCA+TGG + chr5_1:55825925-55825944 None:intergenic 30.0%
ATGAGCATATGTATTCTACA+AGG - chr5_1:55825924-55825943 Msa0738130:three_prime_UTR 30.0%
TAATGAGCAGAATCTACAAT+AGG + chr5_1:55825991-55826010 None:intergenic 30.0%
!! GTTTTAGTCCTTGAAATTGT+AGG - chr5_1:55826021-55826040 Msa0738130:three_prime_UTR 30.0%
TTTAGTCCTTGAAATTGTAG+GGG - chr5_1:55826023-55826042 Msa0738130:three_prime_UTR 30.0%
TCTGTGAACAAGAAAAAAAC+AGG + chr5_1:55826308-55826327 None:intergenic 30.0%
!!! TTTTTTCTTGTTCACAGAGA+TGG - chr5_1:55826310-55826329 Msa0738130:intron 30.0%
! TTTTTCTTGTTCACAGAGAT+GGG - chr5_1:55826311-55826330 Msa0738130:intron 30.0%
ACATGCTAAGCTCAATAATT+TGG - chr5_1:55826541-55826560 Msa0738130:intron 30.0%
GTAATACTCAATCACAGATT+TGG + chr5_1:55826651-55826670 None:intergenic 30.0%
GTGATTGAGTATTACTTCAA+TGG - chr5_1:55826656-55826675 Msa0738130:CDS 30.0%
!!! ACATAGATAGCCTTTTTCAA+TGG - chr5_1:55826720-55826739 Msa0738130:CDS 30.0%
AATGAGGATTCCATTGAAAA+AGG + chr5_1:55826733-55826752 None:intergenic 30.0%
AGTAACAAATTGGAAGAATG+AGG + chr5_1:55826749-55826768 None:intergenic 30.0%
GGATTTGGATTTCATTTAGT+CGG - chr5_1:55826823-55826842 Msa0738130:intron 30.0%
TAAAACTATACATCATGAGC+CGG + chr5_1:55826869-55826888 None:intergenic 30.0%
ACAGATAAATGCTAAGATCT+AGG - chr5_1:55827183-55827202 Msa0738130:intron 30.0%
! AACTTGACAAGGTTTTCTTT+TGG - chr5_1:55827359-55827378 Msa0738130:intron 30.0%
!! ATTTTGTTATCAAGAGAGTC+AGG - chr5_1:55827495-55827514 Msa0738130:CDS 30.0%
! CAACCATTATCTAGGATATA+TGG + chr5_1:55827607-55827626 None:intergenic 30.0%
! CATATATCCTAGATAATGGT+TGG - chr5_1:55827605-55827624 Msa0738130:CDS 30.0%
! TTTTTCTCCAACCATTATCT+AGG + chr5_1:55827615-55827634 None:intergenic 30.0%
TCGATTACAAAGTACATGTT+TGG - chr5_1:55827730-55827749 Msa0738130:CDS 30.0%
AAAGTACATGTTTGGATTGA+CGG - chr5_1:55827738-55827757 Msa0738130:CDS 30.0%
AACATGTCACATATACTTTG+TGG + chr5_1:55827819-55827838 None:intergenic 30.0%
TCACATACTTAGTGTATGTT+TGG + chr5_1:55827867-55827886 None:intergenic 30.0%
AAGGAATGTGATACAGAAAA+AGG - chr5_1:55827974-55827993 Msa0738130:intron 30.0%
AGGAATGTGATACAGAAAAA+GGG - chr5_1:55827975-55827994 Msa0738130:intron 30.0%
!! ATAGGTGCTGGATATATATT+AGG - chr5_1:55828124-55828143 Msa0738130:intron 30.0%
!! AGGTTTTATCCTAGAGTATT+TGG - chr5_1:55828144-55828163 Msa0738130:intron 30.0%
TTTATCGAACCAAATACTCT+AGG + chr5_1:55828156-55828175 None:intergenic 30.0%
CTTAGAAAATCACCGTAAAA+AGG + chr5_1:55828259-55828278 None:intergenic 30.0%
TGCATATGTGGATATTGAAA+TGG - chr5_1:55828495-55828514 Msa0738130:intron 30.0%
AACACATGCATGACAAAATA+TGG - chr5_1:55828553-55828572 Msa0738130:intron 30.0%
TTTAGGTGGAAGAAAAAGAG+TGG + chr5_1:55825691-55825710 None:intergenic 35.0%
TAAAATTTCAGGACACACCT+TGG - chr5_1:55825814-55825833 Msa0738130:three_prime_UTR 35.0%
! AACTTGCTTCAACAAATCCA+AGG + chr5_1:55825834-55825853 None:intergenic 35.0%
! TTTTCTTGTTCACAGAGATG+GGG - chr5_1:55826312-55826331 Msa0738130:intron 35.0%
!!! AGTAGTTTTGTGCTTGAATC+AGG - chr5_1:55826335-55826354 Msa0738130:intron 35.0%
ATGCTGGAATTATTGAGCTT+AGG - chr5_1:55826477-55826496 Msa0738130:intron 35.0%
GACAGGTCTTTACAAATGTT+AGG + chr5_1:55826581-55826600 None:intergenic 35.0%
ACATCAAAACCATCTTCTGA+TGG + chr5_1:55826689-55826708 None:intergenic 35.0%
!!! ATCAGAAGATGGTTTTGATG+TGG - chr5_1:55826688-55826707 Msa0738130:CDS 35.0%
ATTCTTGACTGCGAGTAATT+CGG - chr5_1:55826963-55826982 Msa0738130:intron 35.0%
!! AGGTTGTACATAATGGATAG+AGG + chr5_1:55827142-55827161 None:intergenic 35.0%
ACATTTGAGCTGTTATAGCA+TGG + chr5_1:55827274-55827293 None:intergenic 35.0%
TATGCAGATTAGCATCAATC+TGG + chr5_1:55827326-55827345 None:intergenic 35.0%
TTGTTAGCAGTAACTTGACA+AGG - chr5_1:55827348-55827367 Msa0738130:intron 35.0%
!! ACAAGGTTTTCTTTTGGTGT+TGG - chr5_1:55827365-55827384 Msa0738130:intron 35.0%
!!! GTTGATTGATGGTGACATTT+TGG + chr5_1:55827527-55827546 None:intergenic 35.0%
!! AGCATCAACAAGTTGATTGA+TGG + chr5_1:55827538-55827557 None:intergenic 35.0%
!! ATCAATCAACTTGTTGATGC+TGG - chr5_1:55827537-55827556 Msa0738130:CDS 35.0%
! CATTATCTAGGATATATGGG+TGG + chr5_1:55827603-55827622 None:intergenic 35.0%
CACCCATATATCCTAGATAA+TGG - chr5_1:55827601-55827620 Msa0738130:CDS 35.0%
CAATGAGTTTGGCAAAATCA+CGG + chr5_1:55827791-55827810 None:intergenic 35.0%
TTGTGGATGAACAATGAGTT+TGG + chr5_1:55827802-55827821 None:intergenic 35.0%
CTTAGTGTATGTTTGGTACA+AGG + chr5_1:55827860-55827879 None:intergenic 35.0%
! ACATATTGGTGCTTAGAATC+TGG + chr5_1:55827893-55827912 None:intergenic 35.0%
! TTTTGCAGAACAGAAGATGA+AGG - chr5_1:55827918-55827937 Msa0738130:intron 35.0%
TGATACAGAAAAAGGGTTTC+AGG - chr5_1:55827982-55828001 Msa0738130:intron 35.0%
TAGCAAAAACTTTCGCATTC+CGG + chr5_1:55828063-55828082 None:intergenic 35.0%
GCTCAAACATGTCACATATA+GGG + chr5_1:55828091-55828110 None:intergenic 35.0%
! GACAGTGACATACCTTTTTA+CGG - chr5_1:55828244-55828263 Msa0738130:CDS 35.0%
! TTTTGTCATGCATGTGTTCT+TGG + chr5_1:55828551-55828570 None:intergenic 35.0%
CTAAATCTGAATCACAACTG+CGG - chr5_1:55828603-55828622 Msa0738130:five_prime_UTR 35.0%
TAAATCTGAATCACAACTGC+GGG - chr5_1:55828604-55828623 Msa0738130:five_prime_UTR 35.0%
! TTCGATCATGCTAGTCGTTT+TGG - chr5_1:55826086-55826105 Msa0738130:CDS 40.0%
!! AGACCTGCCCTATAGTTTTA+AGG - chr5_1:55826189-55826208 Msa0738130:CDS 40.0%
AGTCCTTAAAACTATAGGGC+AGG + chr5_1:55826195-55826214 None:intergenic 40.0%
TCTGCAACAATCTTCATTGC+TGG - chr5_1:55826377-55826396 Msa0738130:intron 40.0%
! AGTCACTCTGCTAGTTTCAA+TGG - chr5_1:55826424-55826443 Msa0738130:intron 40.0%
TCATGTCAAAACAGCAATGC+TGG - chr5_1:55826461-55826480 Msa0738130:intron 40.0%
TGGTCAATATGCAAGAGACT+TGG - chr5_1:55826619-55826638 Msa0738130:CDS 40.0%
GGTGTTAAACCATCAGAAGA+TGG - chr5_1:55826677-55826696 Msa0738130:CDS 40.0%
CGGAAAACTTAAAGTCGAGA+TGG - chr5_1:55826843-55826862 Msa0738130:intron 40.0%
AAACTTAAAGTCGAGATGGC+CGG - chr5_1:55826847-55826866 Msa0738130:intron 40.0%
CTCGCAGTCAAGAATCTATA+CGG + chr5_1:55826958-55826977 None:intergenic 40.0%
TACAAGAACCTTCAAGCTAG+TGG - chr5_1:55827462-55827481 Msa0738130:CDS 40.0%
CTTGTTGATGCTGGATTTAG+AGG - chr5_1:55827546-55827565 Msa0738130:CDS 40.0%
ACTGTTACACATGCAGATAC+CGG - chr5_1:55828041-55828060 Msa0738130:intron 40.0%
AACATGTCACATATAGGGTC+TGG + chr5_1:55828086-55828105 None:intergenic 40.0%
GGCTCAAACATGTCACATAT+AGG + chr5_1:55828092-55828111 None:intergenic 40.0%
AGGTGTGTTTGCGTATGTTT+GGG - chr5_1:55828198-55828217 Msa0738130:intron 40.0%
AAATCTGAATCACAACTGCG+GGG - chr5_1:55828605-55828624 Msa0738130:five_prime_UTR 40.0%
CTAACACAACATGAGCAAGC+CGG + chr5_1:55825621-55825640 None:intergenic 45.0%
!! ATTGCTGGATTTGCTGCTCT+TGG - chr5_1:55826392-55826411 Msa0738130:intron 45.0%
! ATGCTAAGATCTAGGACAGC+TGG - chr5_1:55827191-55827210 Msa0738130:intron 45.0%
AGAACCTTCAAGCTAGTGGT+TGG - chr5_1:55827466-55827485 Msa0738130:CDS 45.0%
! TAGACCAACCACTAGCTTGA+AGG + chr5_1:55827473-55827492 None:intergenic 45.0%
TTGATGCTGGATTTAGAGGC+TGG - chr5_1:55827550-55827569 Msa0738130:CDS 45.0%
!! GCTGGTCTTCATTGCTGATA+AGG - chr5_1:55827568-55827587 Msa0738130:CDS 45.0%
TGAGTTTGGCAAAATCACGG+TGG + chr5_1:55827788-55827807 None:intergenic 45.0%
GAGGTGTGTTTGCGTATGTT+TGG - chr5_1:55828197-55828216 Msa0738130:intron 45.0%
! AAAGGTATGTCACTGTCCCA+TGG + chr5_1:55828241-55828260 None:intergenic 45.0%
GTCCAAAACCCTTAGAACTG+GGG + chr5_1:55828319-55828338 None:intergenic 45.0%
CGTCCAAAACCCTTAGAACT+GGG + chr5_1:55828320-55828339 None:intergenic 45.0%
!!! AGTTCTAAGGGTTTTGGACG+CGG - chr5_1:55828320-55828339 Msa0738130:five_prime_UTR 45.0%
!! AAGGTAGGGAAAGAGCACCT+AGG + chr5_1:55825589-55825608 None:intergenic 50.0%
TCCCTACCTTCCCAAATCAC+CGG - chr5_1:55825599-55825618 Msa0738130:three_prime_UTR 50.0%
CATGAGCAAGCCGGTGATTT+GGG + chr5_1:55825612-55825631 None:intergenic 50.0%
ACATGAGCAAGCCGGTGATT+TGG + chr5_1:55825613-55825632 None:intergenic 50.0%
TCTACAAGGAGTCTCTCAGC+TGG - chr5_1:55825938-55825957 Msa0738130:three_prime_UTR 50.0%
CCTCGTCCCCTACAATTTCA+AGG + chr5_1:55826032-55826051 None:intergenic 50.0%
CCTTGAAATTGTAGGGGACG+AGG - chr5_1:55826029-55826048 Msa0738130:three_prime_UTR 50.0%
GTGACATGTTTGAGCCTCAG+AGG - chr5_1:55828096-55828115 Msa0738130:intron 50.0%
GTGTAGCTTCCACGTCAATC+AGG - chr5_1:55828286-55828305 Msa0738130:exon 50.0%
! GTGTTCTGCCCCAGTTCTAA+GGG - chr5_1:55828308-55828327 Msa0738130:five_prime_UTR 50.0%
!!! TGCCCCAGTTCTAAGGGTTT+TGG - chr5_1:55828314-55828333 Msa0738130:five_prime_UTR 50.0%
GCGTCCAAAACCCTTAGAAC+TGG + chr5_1:55828321-55828340 None:intergenic 50.0%
! GCCGGTGATTTGGGAAGGTA+GGG + chr5_1:55825603-55825622 None:intergenic 55.0%
! AGCCGGTGATTTGGGAAGGT+AGG + chr5_1:55825604-55825623 None:intergenic 55.0%
! AGCAAGCCGGTGATTTGGGA+AGG + chr5_1:55825608-55825627 None:intergenic 55.0%
! AGGAGTCTCTCAGCTGGTTC+TGG - chr5_1:55825944-55825963 Msa0738130:three_prime_UTR 55.0%
! TGAGCCTCAGAGGAGAGCAT+AGG - chr5_1:55828106-55828125 Msa0738130:intron 55.0%
AGCACCTATGCTCTCCTCTG+AGG + chr5_1:55828113-55828132 None:intergenic 55.0%
! TCAGAGGAGAGCATAGGTGC+TGG - chr5_1:55828112-55828131 Msa0738130:intron 55.0%
GGTATGTCACTGTCCCATGG+CGG + chr5_1:55828238-55828257 None:intergenic 55.0%
GGCAGAACACCTGATTGACG+TGG + chr5_1:55828298-55828317 None:intergenic 55.0%
GGTGTTCTGCCCCAGTTCTA+AGG - chr5_1:55828307-55828326 Msa0738130:five_prime_UTR 55.0%
! TGAGCGTTTTGCCACCGCCA+TGG - chr5_1:55828221-55828240 Msa0738130:intron 60.0%
! GAGCGTTTTGCCACCGCCAT+GGG - chr5_1:55828222-55828241 Msa0738130:intron 60.0%
ATGTCACTGTCCCATGGCGG+TGG + chr5_1:55828235-55828254 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 55825553 55828648 55825553 ID=Msa0738130;Name=Msa0738130
chr5_1 mRNA 55825553 55828648 55825553 ID=Msa0738130-mRNA-1;Parent=Msa0738130;Name=Msa0738130-mRNA-1;_AED=0.41;_eAED=0.41;_QI=246|1|1|1|0.75|0.6|5|521|296
chr5_1 exon 55825553 55826275 55825553 ID=Msa0738130-mRNA-1:exon:15795;Parent=Msa0738130-mRNA-1
chr5_1 exon 55826612 55826797 55826612 ID=Msa0738130-mRNA-1:exon:15794;Parent=Msa0738130-mRNA-1
chr5_1 exon 55827430 55827874 55827430 ID=Msa0738130-mRNA-1:exon:15793;Parent=Msa0738130-mRNA-1
chr5_1 exon 55828236 55828456 55828236 ID=Msa0738130-mRNA-1:exon:15792;Parent=Msa0738130-mRNA-1
chr5_1 exon 55828566 55828648 55828566 ID=Msa0738130-mRNA-1:exon:15791;Parent=Msa0738130-mRNA-1
chr5_1 five_prime_UTR 55828566 55828648 55828566 ID=Msa0738130-mRNA-1:five_prime_utr;Parent=Msa0738130-mRNA-1
chr5_1 five_prime_UTR 55828294 55828456 55828294 ID=Msa0738130-mRNA-1:five_prime_utr;Parent=Msa0738130-mRNA-1
chr5_1 CDS 55828236 55828293 55828236 ID=Msa0738130-mRNA-1:cds;Parent=Msa0738130-mRNA-1
chr5_1 CDS 55827430 55827874 55827430 ID=Msa0738130-mRNA-1:cds;Parent=Msa0738130-mRNA-1
chr5_1 CDS 55826612 55826797 55826612 ID=Msa0738130-mRNA-1:cds;Parent=Msa0738130-mRNA-1
chr5_1 CDS 55826074 55826275 55826074 ID=Msa0738130-mRNA-1:cds;Parent=Msa0738130-mRNA-1
chr5_1 three_prime_UTR 55825553 55826073 55825553 ID=Msa0738130-mRNA-1:three_prime_utr;Parent=Msa0738130-mRNA-1
Gene Sequence

>Msa0738130

ATGTCTGCTTATGGCCATGAGCATATGTATTCTACAAGGAGTCTCTCAGCTGGTTCTGAGATGGGGAGTAGTTTTGTGCTTGAATCAGGTTTCTACATCACATCATTTTCTGCAACAATCTTCATTGCTGGATTTGCTGCTCTTGGACTTTTATTAGTCACTCTGCTAGTTTCAATGGCAATGATGCTACAATCATGTCAAAACAGCAATGCTGGAATTATTGAGCTTAGGAATATAAATGATGACTATAGTTATTGCAAAATTCATTCTCTACATGCTAAGCTCAATAATTTGGACGAACATAATGTTCCTAACATTTGTAAAGACCTGTCTTTACAATATATCAAAGGTGGTCAATATGCAAGAGACTTGGATTCAACCAAATCTGTGATTGAGTATTACTTCAATGGTGTTAAACCATCAGAAGATGGTTTTGATGTGGTGTTGATAGACATAGATAGCCTTTTTCAATGGAATCCTCATTCTTCCAATTTGTTACTAAGCATTAGCAATTGTATCATCGACGCAAAGAATTTAAAGCGCGTGCTTATGTTAAGAATATACAAGAACCTTCAAGCTAGTGGTTGGTCTATAATTTTGTTATCAAGAGAGTCAGGAACACACCAAAATGTCACCATCAATCAACTTGTTGATGCTGGATTTAGAGGCTGGTCTTCATTGCTGATAAGAACAGAAGATGAAGGATCTACCAAAGCAAATGAATATTTTTCTAGACAAAGGAATGTGATACAGAAAAAGGGTTTCAGGATAAAAAGTATCATAAGCAGTCATGTTGATATTTTGACTGTTACACATGCAGATACCGGAATGCGAAAGTTTTTGCTACCAGACCCTATATGTGACATGTTTGAGCCTCAGAGGAGAGCATAG
Protein sequence

>Msa0738130

MSAYGHEHMYSTRSLSAGSEMGSSFVLESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNAGIIELRNINDDYSYCKIHSLHAKLNNLDEHNVPNICKDLSLQYIKGGQYARDLDSTKSVIEYYFNGVKPSEDGFDVVLIDIDSLFQWNPHSSNLLLSISNCIIDAKNLKRVLMLRIYKNLQASGWSIILLSRESGTHQNVTINQLVDAGFRGWSSLLIRTEDEGSTKANEYFSRQRNVIQKKGFRIKSIISSHVDILTVTHADTGMRKFLLPDPICDMFEPQRRA*