Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817660 | XP_003616581.1 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 1.14e-131 | 376 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817660 | sp|Q8H1D2|WOX5_ARATH | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 5.04e-60 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817660 | G7KBY3 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 5.44e-132 | 376 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0817660 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0817660 | Msa0858140 | 0.910169 | 2.529553e-82 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817660 | MtrunA17_Chr5g0436661 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 1.05e-135 | 376 |
| Msa0817660 | MtrunA17_Chr7g0238431 | 69.841 | 63 | 19 | 0 | 33 | 95 | 8 | 70 | 8.19e-28 | 103 |
| Msa0817660 | MtrunA17_Chr8g0392991 | 64.789 | 71 | 25 | 0 | 25 | 95 | 88 | 158 | 1.59e-26 | 103 |
| Msa0817660 | MtrunA17_Chr4g0043891 | 61.765 | 68 | 26 | 0 | 33 | 100 | 8 | 75 | 6.60e-26 | 99.8 |
| Msa0817660 | MtrunA17_Chr1g0153171 | 65.152 | 66 | 23 | 0 | 33 | 98 | 90 | 155 | 7.09e-26 | 99.4 |
| Msa0817660 | MtrunA17_Chr5g0405351 | 59.459 | 74 | 26 | 2 | 33 | 105 | 30 | 100 | 1.17e-24 | 97.4 |
| Msa0817660 | MtrunA17_Chr4g0031341 | 57.576 | 66 | 28 | 0 | 33 | 98 | 25 | 90 | 9.96e-24 | 94.4 |
| Msa0817660 | MtrunA17_Chr8g0385441 | 69.091 | 55 | 17 | 0 | 44 | 98 | 93 | 147 | 3.15e-20 | 82.8 |
| Msa0817660 | MtrunA17_Chr3g0125111 | 67.273 | 55 | 18 | 0 | 44 | 98 | 91 | 145 | 7.58e-20 | 81.6 |
| Msa0817660 | MtrunA17_Chr7g0223471 | 45.161 | 62 | 34 | 0 | 33 | 94 | 47 | 108 | 1.51e-15 | 73.6 |
| Msa0817660 | MtrunA17_Chr2g0283141 | 45.312 | 64 | 35 | 0 | 33 | 96 | 55 | 118 | 2.03e-15 | 73.2 |
| Msa0817660 | MtrunA17_Chr1g0212511 | 43.333 | 60 | 34 | 0 | 33 | 92 | 85 | 144 | 7.73e-12 | 62.0 |
| Msa0817660 | MtrunA17_Chr7g0222981 | 31.193 | 109 | 60 | 2 | 33 | 127 | 68 | 175 | 7.33e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0817660 | AT3G11260.1 | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 5.13e-61 | 187 |
| Msa0817660 | AT5G05770.1 | 65.487 | 113 | 31 | 3 | 4 | 110 | 2 | 112 | 9.45e-41 | 134 |
| Msa0817660 | AT3G18010.1 | 71.429 | 63 | 18 | 0 | 33 | 95 | 76 | 138 | 7.54e-27 | 104 |
| Msa0817660 | AT2G28610.1 | 65.079 | 63 | 22 | 0 | 33 | 95 | 8 | 70 | 6.19e-26 | 100 |
| Msa0817660 | AT5G59340.1 | 57.333 | 75 | 27 | 1 | 21 | 95 | 7 | 76 | 2.72e-25 | 98.6 |
| Msa0817660 | AT1G46480.2 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 1.19e-24 | 95.5 |
| Msa0817660 | AT1G46480.1 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 7.04e-24 | 94.7 |
| Msa0817660 | AT2G17950.1 | 66.667 | 63 | 20 | 1 | 33 | 94 | 38 | 100 | 9.60e-24 | 95.1 |
| Msa0817660 | AT2G01500.1 | 67.273 | 55 | 18 | 0 | 33 | 87 | 61 | 115 | 1.13e-21 | 89.4 |
| Msa0817660 | AT2G33880.1 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 5.77e-15 | 72.0 |
| Msa0817660 | AT2G33880.2 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 6.57e-15 | 72.0 |
| Msa0817660 | AT5G45980.1 | 46.774 | 62 | 33 | 0 | 33 | 94 | 55 | 116 | 1.72e-14 | 70.5 |
| Msa0817660 | AT4G35550.1 | 42.623 | 61 | 35 | 0 | 33 | 93 | 99 | 159 | 5.73e-12 | 63.2 |
| Msa0817660 | AT1G20700.2 | 35.556 | 90 | 55 | 2 | 19 | 106 | 20 | 108 | 5.82e-12 | 60.8 |
| Msa0817660 | AT1G20700.1 | 35.556 | 90 | 55 | 2 | 19 | 106 | 82 | 170 | 1.19e-11 | 61.2 |
| Msa0817660 | AT1G20700.3 | 38.667 | 75 | 45 | 1 | 19 | 93 | 82 | 155 | 1.23e-11 | 61.2 |
Find 24 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTTAACTTGTTCTGTTGT+TGG | 0.328649 | 5_3:+53020395 | None:intergenic |
| AGAGCAAGAATGTGTTCTAT+TGG | 0.384217 | 5_3:-53020280 | Msa0817660:CDS |
| GATTCACCAAATGAGTTCAA+TGG | 0.392997 | 5_3:+53019826 | None:intergenic |
| AGAGTCTCAATCACTCTATC+AGG | 0.404414 | 5_3:+53019859 | None:intergenic |
| ATGTACAATGGGAGAACAAA+TGG | 0.428304 | 5_3:-53019740 | Msa0817660:CDS |
| AACACCCACCATTGGACTTA+CGG | 0.439714 | 5_3:-53019717 | Msa0817660:CDS |
| GGTTTCAGAATCACAAAGCT+AGG | 0.453724 | 5_3:-53020259 | Msa0817660:CDS |
| TTCTGAATTTGATCAGTGCT+TGG | 0.470346 | 5_3:+53020338 | None:intergenic |
| AGTGGAAGCAGCAGCAGCAC+TGG | 0.493876 | 5_3:-53020440 | Msa0817660:CDS |
| AATATGGAAGAGAGCATGTC+AGG | 0.497698 | 5_3:-53020503 | Msa0817660:CDS |
| TTTGCATACACATGTACAAT+GGG | 0.499345 | 5_3:-53019751 | Msa0817660:CDS |
| AGTAGTTTCCATGGTAAAAG+TGG | 0.503916 | 5_3:-53020458 | Msa0817660:CDS |
| ACAAATGGAACACCCACCAT+TGG | 0.543359 | 5_3:-53019725 | Msa0817660:CDS |
| TCATCTGGAAGTAGTTTCCA+TGG | 0.556114 | 5_3:-53020467 | Msa0817660:CDS |
| GTTTCAGAATCACAAAGCTA+GGG | 0.556495 | 5_3:-53020258 | Msa0817660:CDS |
| GTATGCATGCAAATGAATGC+AGG | 0.569996 | 5_3:-53019786 | Msa0817660:CDS |
| AGCAGCACTGGCACAAAGTG+TGG | 0.575447 | 5_3:-53020428 | Msa0817660:CDS |
| CTCAACCGTAAGTCCAATGG+TGG | 0.576156 | 5_3:+53019712 | None:intergenic |
| GTGAATCAGAATCAGAGAAG+TGG | 0.590084 | 5_3:-53019810 | Msa0817660:CDS |
| CTGGCACAAAGTGTGGTCGT+TGG | 0.591246 | 5_3:-53020421 | Msa0817660:CDS |
| AAACTCAACCGTAAGTCCAA+TGG | 0.615710 | 5_3:+53019709 | None:intergenic |
| GTTTGCATACACATGTACAA+TGG | 0.618786 | 5_3:-53019752 | Msa0817660:CDS |
| TATGCATGCAAATGAATGCA+GGG | 0.627141 | 5_3:-53019785 | Msa0817660:CDS |
| TCAACCGTAAGTCCAATGGT+GGG | 0.711399 | 5_3:+53019713 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTTAAAAAATTATCTTAAA+AGG | - | chr5_3:53020199-53020218 | Msa0817660:CDS | 10.0% |
| !! | TGTTAAAAAATTATCTTAAA+AGG | - | chr5_3:53020199-53020218 | Msa0817660:CDS | 10.0% |
| !!! | AAATTATTTTTCTAACGAGA+TGG | + | chr5_3:53020179-53020198 | None:intergenic | 20.0% |
| !!! | CTTACTAAATTTTTTGCTTT+AGG | - | chr5_3:53020223-53020242 | Msa0817660:CDS | 20.0% |
| !!! | AAATTATTTTTCTAACGAGA+TGG | + | chr5_3:53020179-53020198 | None:intergenic | 20.0% |
| !!! | CTTACTAAATTTTTTGCTTT+AGG | - | chr5_3:53020223-53020242 | Msa0817660:CDS | 20.0% |
| !!! | TCCAATCAACTTAGTTTTTA+TGG | - | chr5_3:53019891-53019910 | Msa0817660:intron | 25.0% |
| !!! | ACCATAAAAACTAAGTTGAT+TGG | + | chr5_3:53019895-53019914 | None:intergenic | 25.0% |
| ! | AATGAATAAATGAGTTGTGT+CGG | + | chr5_3:53020085-53020104 | None:intergenic | 25.0% |
| !!! | TCCAATCAACTTAGTTTTTA+TGG | - | chr5_3:53019891-53019910 | Msa0817660:intron | 25.0% |
| !!! | ACCATAAAAACTAAGTTGAT+TGG | + | chr5_3:53019895-53019914 | None:intergenic | 25.0% |
| ! | AATGAATAAATGAGTTGTGT+CGG | + | chr5_3:53020085-53020104 | None:intergenic | 25.0% |
| !! | AGTTTAACTTGTTCTGTTGT+TGG | + | chr5_3:53019810-53019829 | None:intergenic | 30.0% |
| AGTATCAGATCAAAGTTTCA+AGG | + | chr5_3:53020125-53020144 | None:intergenic | 30.0% | |
| ! | TTCTTTTCCTCATATATTGC+AGG | - | chr5_3:53020298-53020317 | Msa0817660:CDS | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | - | chr5_3:53020299-53020318 | Msa0817660:CDS | 30.0% |
| ! | CTTTTTCCATTGAACTCATT+TGG | - | chr5_3:53020370-53020389 | Msa0817660:CDS | 30.0% |
| TTTGCATACACATGTACAAT+GGG | - | chr5_3:53020451-53020470 | Msa0817660:CDS | 30.0% | |
| !! | AGTTTAACTTGTTCTGTTGT+TGG | + | chr5_3:53019810-53019829 | None:intergenic | 30.0% |
| AGTATCAGATCAAAGTTTCA+AGG | + | chr5_3:53020125-53020144 | None:intergenic | 30.0% | |
| ! | TTCTTTTCCTCATATATTGC+AGG | - | chr5_3:53020298-53020317 | Msa0817660:CDS | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | - | chr5_3:53020299-53020318 | Msa0817660:CDS | 30.0% |
| ! | CTTTTTCCATTGAACTCATT+TGG | - | chr5_3:53020370-53020389 | Msa0817660:CDS | 30.0% |
| TTTGCATACACATGTACAAT+GGG | - | chr5_3:53020451-53020470 | Msa0817660:CDS | 30.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | - | chr5_3:53019744-53019763 | Msa0817660:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | + | chr5_3:53019867-53019886 | None:intergenic | 35.0% | |
| AGAGCAAGAATGTGTTCTAT+TGG | - | chr5_3:53019922-53019941 | Msa0817660:intron | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | - | chr5_3:53019944-53019963 | Msa0817660:intron | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | - | chr5_3:53019986-53020005 | Msa0817660:intron | 35.0% |
| TACATGTTACATCATCGTCA+GGG | + | chr5_3:53020270-53020289 | None:intergenic | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | + | chr5_3:53020271-53020290 | None:intergenic | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | + | chr5_3:53020379-53020398 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | - | chr5_3:53020417-53020436 | Msa0817660:CDS | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | - | chr5_3:53020450-53020469 | Msa0817660:CDS | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | - | chr5_3:53020462-53020481 | Msa0817660:CDS | 35.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | - | chr5_3:53019744-53019763 | Msa0817660:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | + | chr5_3:53019867-53019886 | None:intergenic | 35.0% | |
| AGAGCAAGAATGTGTTCTAT+TGG | - | chr5_3:53019922-53019941 | Msa0817660:intron | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | - | chr5_3:53019944-53019963 | Msa0817660:intron | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | - | chr5_3:53019986-53020005 | Msa0817660:intron | 35.0% |
| TACATGTTACATCATCGTCA+GGG | + | chr5_3:53020270-53020289 | None:intergenic | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | + | chr5_3:53020271-53020290 | None:intergenic | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | + | chr5_3:53020379-53020398 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | - | chr5_3:53020417-53020436 | Msa0817660:CDS | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | - | chr5_3:53020450-53020469 | Msa0817660:CDS | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | - | chr5_3:53020462-53020481 | Msa0817660:CDS | 35.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | - | chr5_3:53019720-53019739 | Msa0817660:CDS | 40.0% |
| TCATCTGGAAGTAGTTTCCA+TGG | - | chr5_3:53019735-53019754 | Msa0817660:CDS | 40.0% | |
| ! | TGACTGAACTTTTCAGAGCA+GGG | - | chr5_3:53019832-53019851 | Msa0817660:CDS | 40.0% |
| GGTTTCAGAATCACAAAGCT+AGG | - | chr5_3:53019943-53019962 | Msa0817660:intron | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | - | chr5_3:53019965-53019984 | Msa0817660:intron | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | - | chr5_3:53020239-53020258 | Msa0817660:CDS | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | + | chr5_3:53020308-53020327 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | + | chr5_3:53020346-53020365 | None:intergenic | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | - | chr5_3:53020392-53020411 | Msa0817660:CDS | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | - | chr5_3:53020416-53020435 | Msa0817660:CDS | 40.0% | |
| AAACTCAACCGTAAGTCCAA+TGG | + | chr5_3:53020496-53020515 | None:intergenic | 40.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | - | chr5_3:53019720-53019739 | Msa0817660:CDS | 40.0% |
| TCATCTGGAAGTAGTTTCCA+TGG | - | chr5_3:53019735-53019754 | Msa0817660:CDS | 40.0% | |
| ! | TGACTGAACTTTTCAGAGCA+GGG | - | chr5_3:53019832-53019851 | Msa0817660:CDS | 40.0% |
| GGTTTCAGAATCACAAAGCT+AGG | - | chr5_3:53019943-53019962 | Msa0817660:intron | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | - | chr5_3:53019965-53019984 | Msa0817660:intron | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | - | chr5_3:53020239-53020258 | Msa0817660:CDS | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | + | chr5_3:53020308-53020327 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | + | chr5_3:53020346-53020365 | None:intergenic | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | - | chr5_3:53020392-53020411 | Msa0817660:CDS | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | - | chr5_3:53020416-53020435 | Msa0817660:CDS | 40.0% | |
| AAACTCAACCGTAAGTCCAA+TGG | + | chr5_3:53020496-53020515 | None:intergenic | 40.0% | |
| !! | TGCTGCTTCCACTTTTACCA+TGG | + | chr5_3:53019755-53019774 | None:intergenic | 45.0% |
| ! | CTGACTGAACTTTTCAGAGC+AGG | - | chr5_3:53019831-53019850 | Msa0817660:CDS | 45.0% |
| ACAAATGGAACACCCACCAT+TGG | - | chr5_3:53020477-53020496 | Msa0817660:CDS | 45.0% | |
| AACACCCACCATTGGACTTA+CGG | - | chr5_3:53020485-53020504 | Msa0817660:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | + | chr5_3:53020492-53020511 | None:intergenic | 45.0% | |
| !! | TGCTGCTTCCACTTTTACCA+TGG | + | chr5_3:53019755-53019774 | None:intergenic | 45.0% |
| ! | CTGACTGAACTTTTCAGAGC+AGG | - | chr5_3:53019831-53019850 | Msa0817660:CDS | 45.0% |
| ACAAATGGAACACCCACCAT+TGG | - | chr5_3:53020477-53020496 | Msa0817660:CDS | 45.0% | |
| AACACCCACCATTGGACTTA+CGG | - | chr5_3:53020485-53020504 | Msa0817660:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | + | chr5_3:53020492-53020511 | None:intergenic | 45.0% | |
| GGCCTCTTAATGCATTGGAC+CGG | - | chr5_3:53020244-53020263 | Msa0817660:CDS | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | + | chr5_3:53020266-53020285 | None:intergenic | 50.0% | |
| CTCAACCGTAAGTCCAATGG+TGG | + | chr5_3:53020493-53020512 | None:intergenic | 50.0% | |
| GGCCTCTTAATGCATTGGAC+CGG | - | chr5_3:53020244-53020263 | Msa0817660:CDS | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | + | chr5_3:53020266-53020285 | None:intergenic | 50.0% | |
| CTCAACCGTAAGTCCAATGG+TGG | + | chr5_3:53020493-53020512 | None:intergenic | 50.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | - | chr5_3:53019774-53019793 | Msa0817660:CDS | 55.0% |
| CTGGCACAAAGTGTGGTCGT+TGG | - | chr5_3:53019781-53019800 | Msa0817660:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | + | chr5_3:53020249-53020268 | None:intergenic | 55.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | - | chr5_3:53019774-53019793 | Msa0817660:CDS | 55.0% |
| CTGGCACAAAGTGTGGTCGT+TGG | - | chr5_3:53019781-53019800 | Msa0817660:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | + | chr5_3:53020249-53020268 | None:intergenic | 55.0% | |
| AGTGGAAGCAGCAGCAGCAC+TGG | - | chr5_3:53019762-53019781 | Msa0817660:CDS | 60.0% | |
| AGTGGAAGCAGCAGCAGCAC+TGG | - | chr5_3:53019762-53019781 | Msa0817660:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 53019702 | 53020522 | 53019702 | ID=Msa0817660;Name=Msa0817660 |
| chr5_3 | mRNA | 53019702 | 53020522 | 53019702 | ID=Msa0817660-mRNA-1;Parent=Msa0817660;Name=Msa0817660-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|184 |
| chr5_3 | exon | 53020171 | 53020522 | 53020171 | ID=Msa0817660-mRNA-1:exon:15973;Parent=Msa0817660-mRNA-1 |
| chr5_3 | exon | 53019702 | 53019904 | 53019702 | ID=Msa0817660-mRNA-1:exon:15972;Parent=Msa0817660-mRNA-1 |
| chr5_3 | CDS | 53020171 | 53020522 | 53020171 | ID=Msa0817660-mRNA-1:cds;Parent=Msa0817660-mRNA-1 |
| chr5_3 | CDS | 53019702 | 53019904 | 53019702 | ID=Msa0817660-mRNA-1:cds;Parent=Msa0817660-mRNA-1 |
| Gene Sequence |
| Protein sequence |
