Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858140 | XP_003616581.1 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 1.14e-131 | 376 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858140 | sp|Q8H1D2|WOX5_ARATH | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 5.04e-60 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858140 | G7KBY3 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 5.44e-132 | 376 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0858140 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0817660 | Msa0858140 | 0.910169 | 2.529553e-82 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858140 | MtrunA17_Chr5g0436661 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 1.05e-135 | 376 |
| Msa0858140 | MtrunA17_Chr7g0238431 | 69.841 | 63 | 19 | 0 | 33 | 95 | 8 | 70 | 8.19e-28 | 103 |
| Msa0858140 | MtrunA17_Chr8g0392991 | 64.789 | 71 | 25 | 0 | 25 | 95 | 88 | 158 | 1.59e-26 | 103 |
| Msa0858140 | MtrunA17_Chr4g0043891 | 61.765 | 68 | 26 | 0 | 33 | 100 | 8 | 75 | 6.60e-26 | 99.8 |
| Msa0858140 | MtrunA17_Chr1g0153171 | 65.152 | 66 | 23 | 0 | 33 | 98 | 90 | 155 | 7.09e-26 | 99.4 |
| Msa0858140 | MtrunA17_Chr5g0405351 | 59.459 | 74 | 26 | 2 | 33 | 105 | 30 | 100 | 1.17e-24 | 97.4 |
| Msa0858140 | MtrunA17_Chr4g0031341 | 57.576 | 66 | 28 | 0 | 33 | 98 | 25 | 90 | 9.96e-24 | 94.4 |
| Msa0858140 | MtrunA17_Chr8g0385441 | 69.091 | 55 | 17 | 0 | 44 | 98 | 93 | 147 | 3.15e-20 | 82.8 |
| Msa0858140 | MtrunA17_Chr3g0125111 | 67.273 | 55 | 18 | 0 | 44 | 98 | 91 | 145 | 7.58e-20 | 81.6 |
| Msa0858140 | MtrunA17_Chr7g0223471 | 45.161 | 62 | 34 | 0 | 33 | 94 | 47 | 108 | 1.51e-15 | 73.6 |
| Msa0858140 | MtrunA17_Chr2g0283141 | 45.312 | 64 | 35 | 0 | 33 | 96 | 55 | 118 | 2.03e-15 | 73.2 |
| Msa0858140 | MtrunA17_Chr1g0212511 | 43.333 | 60 | 34 | 0 | 33 | 92 | 85 | 144 | 7.73e-12 | 62.0 |
| Msa0858140 | MtrunA17_Chr7g0222981 | 31.193 | 109 | 60 | 2 | 33 | 127 | 68 | 175 | 7.33e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0858140 | AT3G11260.1 | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 5.13e-61 | 187 |
| Msa0858140 | AT5G05770.1 | 65.487 | 113 | 31 | 3 | 4 | 110 | 2 | 112 | 9.45e-41 | 134 |
| Msa0858140 | AT3G18010.1 | 71.429 | 63 | 18 | 0 | 33 | 95 | 76 | 138 | 7.54e-27 | 104 |
| Msa0858140 | AT2G28610.1 | 65.079 | 63 | 22 | 0 | 33 | 95 | 8 | 70 | 6.19e-26 | 100 |
| Msa0858140 | AT5G59340.1 | 57.333 | 75 | 27 | 1 | 21 | 95 | 7 | 76 | 2.72e-25 | 98.6 |
| Msa0858140 | AT1G46480.2 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 1.19e-24 | 95.5 |
| Msa0858140 | AT1G46480.1 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 7.04e-24 | 94.7 |
| Msa0858140 | AT2G17950.1 | 66.667 | 63 | 20 | 1 | 33 | 94 | 38 | 100 | 9.60e-24 | 95.1 |
| Msa0858140 | AT2G01500.1 | 67.273 | 55 | 18 | 0 | 33 | 87 | 61 | 115 | 1.13e-21 | 89.4 |
| Msa0858140 | AT2G33880.1 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 5.77e-15 | 72.0 |
| Msa0858140 | AT2G33880.2 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 6.57e-15 | 72.0 |
| Msa0858140 | AT5G45980.1 | 46.774 | 62 | 33 | 0 | 33 | 94 | 55 | 116 | 1.72e-14 | 70.5 |
| Msa0858140 | AT4G35550.1 | 42.623 | 61 | 35 | 0 | 33 | 93 | 99 | 159 | 5.73e-12 | 63.2 |
| Msa0858140 | AT1G20700.2 | 35.556 | 90 | 55 | 2 | 19 | 106 | 20 | 108 | 5.82e-12 | 60.8 |
| Msa0858140 | AT1G20700.1 | 35.556 | 90 | 55 | 2 | 19 | 106 | 82 | 170 | 1.19e-11 | 61.2 |
| Msa0858140 | AT1G20700.3 | 38.667 | 75 | 45 | 1 | 19 | 93 | 82 | 155 | 1.23e-11 | 61.2 |
Find 24 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTTAACTTGTTCTGTTGT+TGG | 0.328649 | 5_4:+55283286 | None:intergenic |
| AGAGCAAGAATGTGTTCTAT+TGG | 0.384217 | 5_4:-55283171 | Msa0858140:CDS |
| GATTCACCAAATGAGTTCAA+TGG | 0.392997 | 5_4:+55282716 | None:intergenic |
| AGAGTCTCAATCACTCTATC+AGG | 0.404414 | 5_4:+55282749 | None:intergenic |
| ATGTACAATGGGAGAACAAA+TGG | 0.428304 | 5_4:-55282630 | Msa0858140:CDS |
| AACACCCACCATTGGACTTA+CGG | 0.439714 | 5_4:-55282607 | Msa0858140:CDS |
| GGTTTCAGAATCACAAAGCT+AGG | 0.453724 | 5_4:-55283150 | Msa0858140:CDS |
| TTCTGAATTTGATCAGTGCT+TGG | 0.470346 | 5_4:+55283229 | None:intergenic |
| AGTGGAAGCAGCAGCAGCAC+TGG | 0.493876 | 5_4:-55283331 | Msa0858140:CDS |
| AATATGGAAGAGAGCATGTC+AGG | 0.497698 | 5_4:-55283394 | Msa0858140:CDS |
| TTTGCATACACATGTACAAT+GGG | 0.499345 | 5_4:-55282641 | Msa0858140:CDS |
| AGTAGTTTCCATGGTAAAAG+TGG | 0.503916 | 5_4:-55283349 | Msa0858140:CDS |
| ACAAATGGAACACCCACCAT+TGG | 0.543359 | 5_4:-55282615 | Msa0858140:CDS |
| TCATCTGGAAGTAGTTTCCA+TGG | 0.556114 | 5_4:-55283358 | Msa0858140:CDS |
| GTTTCAGAATCACAAAGCTA+GGG | 0.556495 | 5_4:-55283149 | Msa0858140:CDS |
| GTATGCATGCAAATGAATGC+AGG | 0.569996 | 5_4:-55282676 | Msa0858140:CDS |
| AGCAGCACTGGCACAAAGTG+TGG | 0.575447 | 5_4:-55283319 | Msa0858140:CDS |
| CTCAACCGTAAGTCCAATGG+TGG | 0.576156 | 5_4:+55282602 | None:intergenic |
| GTGAATCAGAATCAGAGAAG+TGG | 0.590084 | 5_4:-55282700 | Msa0858140:CDS |
| CTGGCACAAAGTGTGGTCGT+TGG | 0.591246 | 5_4:-55283312 | Msa0858140:CDS |
| AAACTCAACCGTAAGTCCAA+TGG | 0.615710 | 5_4:+55282599 | None:intergenic |
| GTTTGCATACACATGTACAA+TGG | 0.618786 | 5_4:-55282642 | Msa0858140:CDS |
| TATGCATGCAAATGAATGCA+GGG | 0.627141 | 5_4:-55282675 | Msa0858140:CDS |
| TCAACCGTAAGTCCAATGGT+GGG | 0.711399 | 5_4:+55282603 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAAAAAAATTATCTTAAA+AGG | - | chr5_4:55283090-55283109 | Msa0858140:CDS | 10.0% |
| !! | GTTAAAAAAATTATCTTAAA+AGG | - | chr5_4:55283090-55283109 | Msa0858140:CDS | 10.0% |
| !!! | AAATTATTTTTCTAACGAGA+TGG | + | chr5_4:55283069-55283088 | None:intergenic | 20.0% |
| !!! | CTTACTAAATTTTTTGCTTT+AGG | - | chr5_4:55283114-55283133 | Msa0858140:CDS | 20.0% |
| !!! | AAATTATTTTTCTAACGAGA+TGG | + | chr5_4:55283069-55283088 | None:intergenic | 20.0% |
| !!! | CTTACTAAATTTTTTGCTTT+AGG | - | chr5_4:55283114-55283133 | Msa0858140:CDS | 20.0% |
| !!! | TCCAATCAACTTAGTTTTTA+TGG | - | chr5_4:55282781-55282800 | Msa0858140:intron | 25.0% |
| !!! | ACCATAAAAACTAAGTTGAT+TGG | + | chr5_4:55282785-55282804 | None:intergenic | 25.0% |
| !!! | TCCAATCAACTTAGTTTTTA+TGG | - | chr5_4:55282781-55282800 | Msa0858140:intron | 25.0% |
| !!! | ACCATAAAAACTAAGTTGAT+TGG | + | chr5_4:55282785-55282804 | None:intergenic | 25.0% |
| !! | AGTTTAACTTGTTCTGTTGT+TGG | + | chr5_4:55282700-55282719 | None:intergenic | 30.0% |
| AAAGAATAAATGAGATGCGT+CGG | + | chr5_4:55282975-55282994 | None:intergenic | 30.0% | |
| AGTATCAGATCAAAGTTTCA+AGG | + | chr5_4:55283015-55283034 | None:intergenic | 30.0% | |
| ! | TTCTTTTCCTCATATATTGC+AGG | - | chr5_4:55283189-55283208 | Msa0858140:CDS | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | - | chr5_4:55283190-55283209 | Msa0858140:CDS | 30.0% |
| ! | CTTTTTCCATTGAACTCATT+TGG | - | chr5_4:55283261-55283280 | Msa0858140:CDS | 30.0% |
| TTTGCATACACATGTACAAT+GGG | - | chr5_4:55283342-55283361 | Msa0858140:CDS | 30.0% | |
| !! | AGTTTAACTTGTTCTGTTGT+TGG | + | chr5_4:55282700-55282719 | None:intergenic | 30.0% |
| AAAGAATAAATGAGATGCGT+CGG | + | chr5_4:55282975-55282994 | None:intergenic | 30.0% | |
| AGTATCAGATCAAAGTTTCA+AGG | + | chr5_4:55283015-55283034 | None:intergenic | 30.0% | |
| ! | TTCTTTTCCTCATATATTGC+AGG | - | chr5_4:55283189-55283208 | Msa0858140:CDS | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | - | chr5_4:55283190-55283209 | Msa0858140:CDS | 30.0% |
| ! | CTTTTTCCATTGAACTCATT+TGG | - | chr5_4:55283261-55283280 | Msa0858140:CDS | 30.0% |
| TTTGCATACACATGTACAAT+GGG | - | chr5_4:55283342-55283361 | Msa0858140:CDS | 30.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | - | chr5_4:55282634-55282653 | Msa0858140:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | + | chr5_4:55282757-55282776 | None:intergenic | 35.0% | |
| AGAGCAAGAATGTGTTCTAT+TGG | - | chr5_4:55282812-55282831 | Msa0858140:intron | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | - | chr5_4:55282834-55282853 | Msa0858140:intron | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | - | chr5_4:55282876-55282895 | Msa0858140:intron | 35.0% |
| TACATGTTACATCATCGTCA+GGG | + | chr5_4:55283161-55283180 | None:intergenic | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | + | chr5_4:55283162-55283181 | None:intergenic | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | + | chr5_4:55283270-55283289 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | - | chr5_4:55283308-55283327 | Msa0858140:CDS | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | - | chr5_4:55283341-55283360 | Msa0858140:CDS | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | - | chr5_4:55283353-55283372 | Msa0858140:CDS | 35.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | - | chr5_4:55282634-55282653 | Msa0858140:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | + | chr5_4:55282757-55282776 | None:intergenic | 35.0% | |
| AGAGCAAGAATGTGTTCTAT+TGG | - | chr5_4:55282812-55282831 | Msa0858140:intron | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | - | chr5_4:55282834-55282853 | Msa0858140:intron | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | - | chr5_4:55282876-55282895 | Msa0858140:intron | 35.0% |
| TACATGTTACATCATCGTCA+GGG | + | chr5_4:55283161-55283180 | None:intergenic | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | + | chr5_4:55283162-55283181 | None:intergenic | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | + | chr5_4:55283270-55283289 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | - | chr5_4:55283308-55283327 | Msa0858140:CDS | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | - | chr5_4:55283341-55283360 | Msa0858140:CDS | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | - | chr5_4:55283353-55283372 | Msa0858140:CDS | 35.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | - | chr5_4:55282610-55282629 | Msa0858140:CDS | 40.0% |
| TCATCTGGAAGTAGTTTCCA+TGG | - | chr5_4:55282625-55282644 | Msa0858140:CDS | 40.0% | |
| ! | TGACTGAACTTTTCAGAGCA+GGG | - | chr5_4:55282722-55282741 | Msa0858140:CDS | 40.0% |
| GGTTTCAGAATCACAAAGCT+AGG | - | chr5_4:55282833-55282852 | Msa0858140:intron | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | - | chr5_4:55282855-55282874 | Msa0858140:intron | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | - | chr5_4:55283130-55283149 | Msa0858140:CDS | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | + | chr5_4:55283199-55283218 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | + | chr5_4:55283237-55283256 | None:intergenic | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | - | chr5_4:55283283-55283302 | Msa0858140:CDS | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | - | chr5_4:55283307-55283326 | Msa0858140:CDS | 40.0% | |
| AAACTCAACCGTAAGTCCAA+TGG | + | chr5_4:55283387-55283406 | None:intergenic | 40.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | - | chr5_4:55282610-55282629 | Msa0858140:CDS | 40.0% |
| TCATCTGGAAGTAGTTTCCA+TGG | - | chr5_4:55282625-55282644 | Msa0858140:CDS | 40.0% | |
| ! | TGACTGAACTTTTCAGAGCA+GGG | - | chr5_4:55282722-55282741 | Msa0858140:CDS | 40.0% |
| GGTTTCAGAATCACAAAGCT+AGG | - | chr5_4:55282833-55282852 | Msa0858140:intron | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | - | chr5_4:55282855-55282874 | Msa0858140:intron | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | - | chr5_4:55283130-55283149 | Msa0858140:CDS | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | + | chr5_4:55283199-55283218 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | + | chr5_4:55283237-55283256 | None:intergenic | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | - | chr5_4:55283283-55283302 | Msa0858140:CDS | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | - | chr5_4:55283307-55283326 | Msa0858140:CDS | 40.0% | |
| AAACTCAACCGTAAGTCCAA+TGG | + | chr5_4:55283387-55283406 | None:intergenic | 40.0% | |
| !! | TGCTGCTTCCACTTTTACCA+TGG | + | chr5_4:55282645-55282664 | None:intergenic | 45.0% |
| ! | CTGACTGAACTTTTCAGAGC+AGG | - | chr5_4:55282721-55282740 | Msa0858140:CDS | 45.0% |
| ACAAATGGAACACCCACCAT+TGG | - | chr5_4:55283368-55283387 | Msa0858140:CDS | 45.0% | |
| AACACCCACCATTGGACTTA+CGG | - | chr5_4:55283376-55283395 | Msa0858140:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | + | chr5_4:55283383-55283402 | None:intergenic | 45.0% | |
| !! | TGCTGCTTCCACTTTTACCA+TGG | + | chr5_4:55282645-55282664 | None:intergenic | 45.0% |
| ! | CTGACTGAACTTTTCAGAGC+AGG | - | chr5_4:55282721-55282740 | Msa0858140:CDS | 45.0% |
| ACAAATGGAACACCCACCAT+TGG | - | chr5_4:55283368-55283387 | Msa0858140:CDS | 45.0% | |
| AACACCCACCATTGGACTTA+CGG | - | chr5_4:55283376-55283395 | Msa0858140:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | + | chr5_4:55283383-55283402 | None:intergenic | 45.0% | |
| GGCCTCTTAATGCATTGGAC+CGG | - | chr5_4:55283135-55283154 | Msa0858140:CDS | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | + | chr5_4:55283157-55283176 | None:intergenic | 50.0% | |
| CTCAACCGTAAGTCCAATGG+TGG | + | chr5_4:55283384-55283403 | None:intergenic | 50.0% | |
| GGCCTCTTAATGCATTGGAC+CGG | - | chr5_4:55283135-55283154 | Msa0858140:CDS | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | + | chr5_4:55283157-55283176 | None:intergenic | 50.0% | |
| CTCAACCGTAAGTCCAATGG+TGG | + | chr5_4:55283384-55283403 | None:intergenic | 50.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | - | chr5_4:55282664-55282683 | Msa0858140:CDS | 55.0% |
| CTGGCACAAAGTGTGGTCGT+TGG | - | chr5_4:55282671-55282690 | Msa0858140:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | + | chr5_4:55283140-55283159 | None:intergenic | 55.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | - | chr5_4:55282664-55282683 | Msa0858140:CDS | 55.0% |
| CTGGCACAAAGTGTGGTCGT+TGG | - | chr5_4:55282671-55282690 | Msa0858140:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | + | chr5_4:55283140-55283159 | None:intergenic | 55.0% | |
| AGTGGAAGCAGCAGCAGCAC+TGG | - | chr5_4:55282652-55282671 | Msa0858140:CDS | 60.0% | |
| AGTGGAAGCAGCAGCAGCAC+TGG | - | chr5_4:55282652-55282671 | Msa0858140:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 55282592 | 55283413 | 55282592 | ID=Msa0858140;Name=Msa0858140 |
| chr5_4 | mRNA | 55282592 | 55283413 | 55282592 | ID=Msa0858140-mRNA-1;Parent=Msa0858140;Name=Msa0858140-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|184 |
| chr5_4 | exon | 55283062 | 55283413 | 55283062 | ID=Msa0858140-mRNA-1:exon:16972;Parent=Msa0858140-mRNA-1 |
| chr5_4 | exon | 55282592 | 55282794 | 55282592 | ID=Msa0858140-mRNA-1:exon:16971;Parent=Msa0858140-mRNA-1 |
| chr5_4 | CDS | 55283062 | 55283413 | 55283062 | ID=Msa0858140-mRNA-1:cds;Parent=Msa0858140-mRNA-1 |
| chr5_4 | CDS | 55282592 | 55282794 | 55282592 | ID=Msa0858140-mRNA-1:cds;Parent=Msa0858140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
