AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0818590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0818590 MtrunA17_Chr1g0193121 63.830 47 14 1 52 95 28 74 1.01e-11 60.5
Msa0818590 MtrunA17_Chr3g0113951 63.415 41 15 0 55 95 143 183 6.96e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCATCACATTATCTAAAA+TGG 0.146328 5_3:+54540827 Msa0818590:three_prime_UTR
AAACTTTCTCCACTTCATTT+TGG 0.184793 5_3:+54541033 Msa0818590:three_prime_UTR
CGTTGAAGGATCATTTCTTT+TGG 0.236974 5_3:-54538347 None:intergenic
TTGTTATTTGAAATAGTTAC+TGG 0.291033 5_3:+54540763 Msa0818590:three_prime_UTR
TCTTGTTAAGTTGCTAGAAT+TGG 0.311781 5_3:-54540877 None:intergenic
ATAATTGCTCAGTTGAAAAC+TGG 0.318836 5_3:+54540806 Msa0818590:three_prime_UTR
AGTCGGTCTCTTCTCCGAAT+TGG 0.322314 5_3:-54540951 None:intergenic
TTTCAGTTGATCGCTGTCAT+TGG 0.359585 5_3:+54540314 Msa0818590:CDS
GTCGGTCTCTTCTCCGAATT+GGG 0.384719 5_3:-54540950 None:intergenic
AGAACTCATGCATATGTTGT+AGG 0.409419 5_3:-54540585 None:intergenic
TACTGGTCGAATTCCTTGTT+CGG 0.410966 5_3:+54540780 Msa0818590:three_prime_UTR
CAGCTATAAACATTACTTGC+TGG 0.415501 5_3:-54540733 None:intergenic
CCATTGCAAATTCTAAGTCT+TGG 0.440583 5_3:-54540549 None:intergenic
TTCAGTTGATCGCTGTCATT+GGG 0.441038 5_3:+54540315 Msa0818590:CDS
AAGAAGCTGACCAATCGAAC+AGG 0.447048 5_3:+54540523 Msa0818590:CDS
GGCATCACATTATCTAAAAT+GGG 0.449479 5_3:+54540828 Msa0818590:three_prime_UTR
CTTAGAATTTGCAATGGGAA+TGG 0.464104 5_3:+54540555 Msa0818590:CDS
GGACAAACCTCTCAAGGAAA+TGG 0.465375 5_3:+54540849 Msa0818590:three_prime_UTR
CCCTTCCGTTATATATGCTC+TGG 0.470057 5_3:+54538795 Msa0818590:CDS
GTCTTGGTTCCCTGTTCGAT+TGG 0.470710 5_3:-54540533 None:intergenic
TCGACCCTTTCCACCGTTGA+AGG 0.472288 5_3:-54538361 None:intergenic
TGATCCTTCAACGGTGGAAA+GGG 0.483036 5_3:+54538357 Msa0818590:CDS
GTTGATCGCTGTCATTGGGT+TGG 0.490879 5_3:+54540319 Msa0818590:CDS
CAAGACTTAGAATTTGCAAT+GGG 0.495099 5_3:+54540550 Msa0818590:CDS
CAATTTGACAAATCATCAAA+AGG 0.506672 5_3:-54540277 None:intergenic
TTCGGCTGAATCTATTTCGT+CGG 0.524052 5_3:-54540664 None:intergenic
ATGATCCTTCAACGGTGGAA+AGG 0.529383 5_3:+54538356 Msa0818590:CDS
GGATCCACCATTTCCTTGAG+AGG 0.537844 5_3:-54540856 None:intergenic
TTGGGTTGGAGAAGAATGCT+TGG 0.543810 5_3:+54540333 Msa0818590:CDS
CTCAGCCCACCAAAATGAAG+TGG 0.549286 5_3:-54541042 None:intergenic
AAAATGGGACAAACCTCTCA+AGG 0.552810 5_3:+54540843 Msa0818590:three_prime_UTR
ACCAGAGCATATATAACGGA+AGG 0.565928 5_3:-54538796 None:intergenic
CCAAGACTTAGAATTTGCAA+TGG 0.571715 5_3:+54540549 Msa0818590:CDS
GCAAGTAATGTTTATAGCTG+TGG 0.572774 5_3:+54540736 Msa0818590:three_prime_UTR
AGCGTGGTCATCGGGTTATG+TGG 0.575113 5_3:-54540616 None:intergenic
AAGAAATGATCCTTCAACGG+TGG 0.581360 5_3:+54538351 Msa0818590:exon
TGACCACGCTGCTAAAATCT+CGG 0.583117 5_3:+54540629 Msa0818590:three_prime_UTR
CAAACCTCTCAAGGAAATGG+TGG 0.583685 5_3:+54540852 Msa0818590:three_prime_UTR
TAATTGCTCAGTTGAAAACT+GGG 0.584174 5_3:+54540807 Msa0818590:three_prime_UTR
TCACTGATTTCATTCATAGT+CGG 0.585994 5_3:-54540968 None:intergenic
ATTTCTTGCAAGAAAAGCAA+AGG 0.588297 5_3:+54538309 Msa0818590:five_prime_UTR
AATTTCAAAAGATCTGAGAC+CGG 0.588399 5_3:+54539650 Msa0818590:CDS
TTGCAAGAAAAGCAAAGGTG+AGG 0.600501 5_3:+54538314 Msa0818590:five_prime_UTR
GAAGGGTCGTGTAGCTGCAG+AGG 0.601707 5_3:-54538778 None:intergenic
TGTAACCAGAGCATATATAA+CGG 0.602876 5_3:-54538800 None:intergenic
GAGCAATTATCAACCGAACA+AGG 0.610422 5_3:-54540793 None:intergenic
CAAAAGAAATGATCCTTCAA+CGG 0.613619 5_3:+54538348 Msa0818590:exon
TAACAAGAAGATCAATCATG+TGG 0.619730 5_3:+54540892 Msa0818590:three_prime_UTR
CAACGGTGGAAAGGGTCGAA+TGG 0.631199 5_3:+54538365 Msa0818590:CDS
TATATATGCTCTGGTTACAC+AGG 0.638021 5_3:+54538804 Msa0818590:CDS
AGAAGCTGACCAATCGAACA+GGG 0.669085 5_3:+54540524 Msa0818590:CDS
CCAGAGCATATATAACGGAA+GGG 0.681449 5_3:-54538795 None:intergenic
GTGTAGCTGCAGAGGCACTG+AGG 0.736669 5_3:-54538770 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATCTTGTTTTTACATAAAA+AGG + chr5_3:54538416-54538435 Msa0818590:intron 15.0%
!!! ATCTTGTTTTTACATAAAAA+GGG + chr5_3:54538417-54538436 Msa0818590:intron 15.0%
!! TAAATAGCTTATATATTCAA+AGG + chr5_3:54538494-54538513 Msa0818590:intron 15.0%
!! ATAAAAATAAAACCAAGTTA+TGG - chr5_3:54539984-54540003 None:intergenic 15.0%
!! TATTTATATAGTTTCAACCA+TGG - chr5_3:54538480-54538499 None:intergenic 20.0%
!! ATTCAATTATTTCACTAACA+AGG - chr5_3:54538619-54538638 None:intergenic 20.0%
!! TTGGATAAACAAATATTAGT+AGG + chr5_3:54538916-54538935 Msa0818590:intron 20.0%
!! ATAAACAAATATTAGTAGGT+CGG + chr5_3:54538920-54538939 Msa0818590:intron 20.0%
!!! GATAAGTCTAAATTTTAAGA+TGG + chr5_3:54539034-54539053 Msa0818590:intron 20.0%
!! AAATAACTTCAACACAATTA+AGG - chr5_3:54539125-54539144 None:intergenic 20.0%
!! ACATCAAATTAAGTCAATAA+AGG - chr5_3:54539747-54539766 None:intergenic 20.0%
!! ATTTAATAGATATGCTTGAT+GGG + chr5_3:54540029-54540048 Msa0818590:intron 20.0%
!!! CATTTACTTTGTTTCATATT+AGG + chr5_3:54540083-54540102 Msa0818590:intron 20.0%
!!! TTTTGATGATTTGTCAAATT+GGG + chr5_3:54540279-54540298 Msa0818590:CDS 20.0%
!!! TTGTTATTTGAAATAGTTAC+TGG + chr5_3:54540763-54540782 Msa0818590:three_prime_UTR 20.0%
! AGAGACAGTTAAAAGATTAA+AGG - chr5_3:54538396-54538415 None:intergenic 25.0%
! ATAGCTTATATATTCAAAGG+TGG + chr5_3:54538497-54538516 Msa0818590:intron 25.0%
!!! TCAGACAAATATTTTTGGTT+TGG + chr5_3:54538558-54538577 Msa0818590:intron 25.0%
! AGTTCAGCTAGATTAATTTA+TGG - chr5_3:54538723-54538742 None:intergenic 25.0%
! AAAGACATCAATCAAAGAAA+AGG - chr5_3:54538882-54538901 None:intergenic 25.0%
!!! TCTTTTGAGGGAAAAAAAAT+TGG + chr5_3:54538897-54538916 Msa0818590:intron 25.0%
!!! GTTTTGAACCACTTATTATA+AGG + chr5_3:54538963-54538982 Msa0818590:intron 25.0%
! TAACAACACCTTATAATAAG+TGG - chr5_3:54538974-54538993 None:intergenic 25.0%
! TTTAGACTTATCTTCTAACT+TGG - chr5_3:54539026-54539045 None:intergenic 25.0%
!! TAACTTGCATGTATAAATGA+TGG - chr5_3:54539212-54539231 None:intergenic 25.0%
! AGTTACTAAGATTTCAAAGT+TGG + chr5_3:54539227-54539246 Msa0818590:intron 25.0%
! ATTTGATAAACAGACATCAA+AGG + chr5_3:54539292-54539311 Msa0818590:intron 25.0%
!!! AATATTCTGCCCTTATTTTA+AGG - chr5_3:54539318-54539337 None:intergenic 25.0%
! AAATTTGATATTAGTGACGT+CGG - chr5_3:54539502-54539521 None:intergenic 25.0%
!!! GAGTTTTTTTTGCTTGTTAA+AGG + chr5_3:54539589-54539608 Msa0818590:intron 25.0%
!! GAAAAATGCTTTATTAGTAC+CGG - chr5_3:54539672-54539691 None:intergenic 25.0%
!! GCATTTTTCACTTATTCATT+AGG + chr5_3:54539683-54539702 Msa0818590:CDS 25.0%
!! GTGATTTTCTTGATTAAATC+AGG + chr5_3:54539928-54539947 Msa0818590:intron 25.0%
! ATCTTAATACTACCATAACT+TGG + chr5_3:54539969-54539988 Msa0818590:intron 25.0%
! CATTTAATAGATATGCTTGA+TGG + chr5_3:54540028-54540047 Msa0818590:intron 25.0%
! CAATTTGACAAATCATCAAA+AGG - chr5_3:54540280-54540299 None:intergenic 25.0%
!!! CTTTTGATGATTTGTCAAAT+TGG + chr5_3:54540278-54540297 Msa0818590:CDS 25.0%
! CTTTTCTTGCAAGAAATACT+TGG - chr5_3:54538306-54538325 None:intergenic 30.0%
ATTTCTTGCAAGAAAAGCAA+AGG + chr5_3:54538309-54538328 Msa0818590:five_prime_UTR 30.0%
! GTTGAAGGATCATTTCTTTT+GGG - chr5_3:54538349-54538368 None:intergenic 30.0%
CAAAAGAAATGATCCTTCAA+CGG + chr5_3:54538348-54538367 Msa0818590:exon 30.0%
!! TTGAGATTCAAGTTGTGAAT+TGG - chr5_3:54538449-54538468 None:intergenic 30.0%
! GAATCTCAATTTGATAACCA+TGG + chr5_3:54538460-54538479 Msa0818590:intron 30.0%
! AAGGCTCAGACAAATATTTT+TGG + chr5_3:54538553-54538572 Msa0818590:intron 30.0%
ATTTCACTAACAAGGCAAAA+TGG - chr5_3:54538611-54538630 None:intergenic 30.0%
TCAGCTAGATTAATTTATGG+TGG - chr5_3:54538720-54538739 None:intergenic 30.0%
TGTAACCAGAGCATATATAA+CGG - chr5_3:54538803-54538822 None:intergenic 30.0%
! TCTTTGATTGATGTCTTTTG+AGG + chr5_3:54538884-54538903 Msa0818590:intron 30.0%
! CTTTGATTGATGTCTTTTGA+GGG + chr5_3:54538885-54538904 Msa0818590:intron 30.0%
!!! GGTGATTATGTTAGTTTTCA+GGG + chr5_3:54538941-54538960 Msa0818590:intron 30.0%
CAATTAAGGAGAATAGATCA+TGG - chr5_3:54539111-54539130 None:intergenic 30.0%
ACATCAAAGGCCTTAAAATA+AGG + chr5_3:54539305-54539324 Msa0818590:intron 30.0%
CATCAAAGGCCTTAAAATAA+GGG + chr5_3:54539306-54539325 Msa0818590:intron 30.0%
AATATTGTTAAAACGAAGCG+TGG + chr5_3:54539332-54539351 Msa0818590:intron 30.0%
! AATTTCAAAAGATCTGAGAC+CGG + chr5_3:54539650-54539669 Msa0818590:CDS 30.0%
! TTTGTACTATGTGCTTTGTT+TGG + chr5_3:54539874-54539893 Msa0818590:intron 30.0%
ATTAAATGTGTGCTTGTGTT+TGG - chr5_3:54540016-54540035 None:intergenic 30.0%
TGCATTAGTGAAAGTAAATG+TGG - chr5_3:54540147-54540166 None:intergenic 30.0%
! TGCATTTTTGCATACAAGTT+TGG + chr5_3:54540456-54540475 Msa0818590:intron 30.0%
CAAGACTTAGAATTTGCAAT+GGG + chr5_3:54540550-54540569 Msa0818590:CDS 30.0%
!!! ACTACCATCAGACTTTTTTT+CGG - chr5_3:54540685-54540704 None:intergenic 30.0%
ATAATTGCTCAGTTGAAAAC+TGG + chr5_3:54540806-54540825 Msa0818590:three_prime_UTR 30.0%
TAATTGCTCAGTTGAAAACT+GGG + chr5_3:54540807-54540826 Msa0818590:three_prime_UTR 30.0%
! GGCATCACATTATCTAAAAT+GGG + chr5_3:54540828-54540847 Msa0818590:three_prime_UTR 30.0%
! TCTTGTTAAGTTGCTAGAAT+TGG - chr5_3:54540880-54540899 None:intergenic 30.0%
TAACAAGAAGATCAATCATG+TGG + chr5_3:54540892-54540911 Msa0818590:three_prime_UTR 30.0%
! AGTTTTTGTTGATCCCAATT+CGG + chr5_3:54540937-54540956 Msa0818590:three_prime_UTR 30.0%
!! TCACTGATTTCATTCATAGT+CGG - chr5_3:54540971-54540990 None:intergenic 30.0%
! AGTTTTCGAACTGTAAAATC+AGG - chr5_3:54541018-54541037 None:intergenic 30.0%
! CGTTGAAGGATCATTTCTTT+TGG - chr5_3:54538350-54538369 None:intergenic 35.0%
ATAAGATGAAGAACCACTTG+AGG - chr5_3:54538588-54538607 None:intergenic 35.0%
!! TAGCTGAACTCATGTTTTGA+AGG + chr5_3:54538733-54538752 Msa0818590:intron 35.0%
!! AGCTGAACTCATGTTTTGAA+GGG + chr5_3:54538734-54538753 Msa0818590:intron 35.0%
TATATATGCTCTGGTTACAC+AGG + chr5_3:54538804-54538823 Msa0818590:CDS 35.0%
!!! CGGTGATTATGTTAGTTTTC+AGG + chr5_3:54538940-54538959 Msa0818590:intron 35.0%
TTAGTCCTCACCTTATTAGT+TGG + chr5_3:54538991-54539010 Msa0818590:intron 35.0%
ACAAACCAACTAATAAGGTG+AGG - chr5_3:54538999-54539018 None:intergenic 35.0%
!! CACCTTATTAGTTGGTTTGT+AGG + chr5_3:54538999-54539018 Msa0818590:intron 35.0%
GTCCTACAAACCAACTAATA+AGG - chr5_3:54539004-54539023 None:intergenic 35.0%
GTGGTTCAACAATTGATGAA+GGG - chr5_3:54539072-54539091 None:intergenic 35.0%
! TTGGTAAAACTTTTGCAGAC+AGG + chr5_3:54539246-54539265 Msa0818590:intron 35.0%
GAGACAATTCAATTTCAGCT+TGG - chr5_3:54539480-54539499 None:intergenic 35.0%
ACAACTGCAAAACTCTTGAA+TGG - chr5_3:54539556-54539575 None:intergenic 35.0%
!!! AGTTTTGCAGTTGTTTTAGC+TGG + chr5_3:54539562-54539581 Msa0818590:intron 35.0%
ACAATGAAATGACGAGAAGT+AGG - chr5_3:54539722-54539741 None:intergenic 35.0%
GAAAGTAAATGTGGTTAGCA+TGG - chr5_3:54540138-54540157 None:intergenic 35.0%
TTCAGCATCTATAGCCAAAA+GGG - chr5_3:54540197-54540216 None:intergenic 35.0%
TTTCAGCATCTATAGCCAAA+AGG - chr5_3:54540198-54540217 None:intergenic 35.0%
TGGCTATAGATGCTGAAATA+TGG + chr5_3:54540200-54540219 Msa0818590:intron 35.0%
GAAATATGGTTTCTTTGTGC+AGG + chr5_3:54540214-54540233 Msa0818590:intron 35.0%
CGTGTAGAACAAGAAAAAAG+AGG + chr5_3:54540421-54540440 Msa0818590:intron 35.0%
CCATTGCAAATTCTAAGTCT+TGG - chr5_3:54540552-54540571 None:intergenic 35.0%
CCAAGACTTAGAATTTGCAA+TGG + chr5_3:54540549-54540568 Msa0818590:CDS 35.0%
CTTAGAATTTGCAATGGGAA+TGG + chr5_3:54540555-54540574 Msa0818590:CDS 35.0%
AGAACTCATGCATATGTTGT+AGG - chr5_3:54540588-54540607 None:intergenic 35.0%
CAGCTATAAACATTACTTGC+TGG - chr5_3:54540736-54540755 None:intergenic 35.0%
GCAAGTAATGTTTATAGCTG+TGG + chr5_3:54540736-54540755 Msa0818590:three_prime_UTR 35.0%
GGGCATCACATTATCTAAAA+TGG + chr5_3:54540827-54540846 Msa0818590:three_prime_UTR 35.0%
TTGCAAGAAAAGCAAAGGTG+AGG + chr5_3:54538314-54538333 Msa0818590:five_prime_UTR 40.0%
AAGAAATGATCCTTCAACGG+TGG + chr5_3:54538351-54538370 Msa0818590:exon 40.0%
ACACTAACACGTTACACATG+CGG - chr5_3:54538646-54538665 None:intergenic 40.0%
! CCAGAGCATATATAACGGAA+GGG - chr5_3:54538798-54538817 None:intergenic 40.0%
! ACCAGAGCATATATAACGGA+AGG - chr5_3:54538799-54538818 None:intergenic 40.0%
GGTGGTTCAACAATTGATGA+AGG - chr5_3:54539073-54539092 None:intergenic 40.0%
TCATGGATCATGGACAATGT+GGG - chr5_3:54539094-54539113 None:intergenic 40.0%
ATCATGGATCATGGACAATG+TGG - chr5_3:54539095-54539114 None:intergenic 40.0%
GGAGAATAGATCATGGATCA+TGG - chr5_3:54539104-54539123 None:intergenic 40.0%
TTTGCAGACAGGATCAAGAA+TGG + chr5_3:54539257-54539276 Msa0818590:intron 40.0%
AATAGAGTGCACCGTTGAAT+CGG - chr5_3:54539390-54539409 None:intergenic 40.0%
!!! AGTGACCAGTGGTTTTGAAT+GGG - chr5_3:54539417-54539436 None:intergenic 40.0%
TCAGCTTATGACAACCAAGA+TGG + chr5_3:54539451-54539470 Msa0818590:intron 40.0%
! TTTCAGCTTGGTAACCATCT+TGG - chr5_3:54539468-54539487 None:intergenic 40.0%
AAATGACGAGAAGTAGGTTG+TGG - chr5_3:54539716-54539735 None:intergenic 40.0%
AGCTACAATTTCCACTTCCA+GGG + chr5_3:54539796-54539815 Msa0818590:intron 40.0%
TTTCAGTTGATCGCTGTCAT+TGG + chr5_3:54540314-54540333 Msa0818590:CDS 40.0%
TTCAGTTGATCGCTGTCATT+GGG + chr5_3:54540315-54540334 Msa0818590:CDS 40.0%
TCTACACGCAACACACAAAA+GGG - chr5_3:54540409-54540428 None:intergenic 40.0%
TTCTACACGCAACACACAAA+AGG - chr5_3:54540410-54540429 None:intergenic 40.0%
!! GATTGGTCAGCTTCTTTTAC+TGG - chr5_3:54540519-54540538 None:intergenic 40.0%
TTCGGCTGAATCTATTTCGT+CGG - chr5_3:54540667-54540686 None:intergenic 40.0%
TCAGCCGAAAAAAAGTCTGA+TGG + chr5_3:54540678-54540697 Msa0818590:three_prime_UTR 40.0%
TACTGGTCGAATTCCTTGTT+CGG + chr5_3:54540780-54540799 Msa0818590:three_prime_UTR 40.0%
GAGCAATTATCAACCGAACA+AGG - chr5_3:54540796-54540815 None:intergenic 40.0%
AAAATGGGACAAACCTCTCA+AGG + chr5_3:54540843-54540862 Msa0818590:three_prime_UTR 40.0%
ATGATCCTTCAACGGTGGAA+AGG + chr5_3:54538356-54538375 Msa0818590:CDS 45.0%
TGATCCTTCAACGGTGGAAA+GGG + chr5_3:54538357-54538376 Msa0818590:CDS 45.0%
GAAAGCCATGCAGTTGTTCA+AGG + chr5_3:54538534-54538553 Msa0818590:intron 45.0%
CCCTTCCGTTATATATGCTC+TGG + chr5_3:54538795-54538814 Msa0818590:CDS 45.0%
!!! TGACCAGTGGTTTTGAATGG+GGG - chr5_3:54539415-54539434 None:intergenic 45.0%
!!! GTGACCAGTGGTTTTGAATG+GGG - chr5_3:54539416-54539435 None:intergenic 45.0%
!!! GAGTGACCAGTGGTTTTGAA+TGG - chr5_3:54539418-54539437 None:intergenic 45.0%
GAGCTACAATTTCCACTTCC+AGG + chr5_3:54539795-54539814 Msa0818590:intron 45.0%
! ATCGATCTTTTCCCTGGAAG+TGG - chr5_3:54539810-54539829 None:intergenic 45.0%
! CACCTAGCTAGTTACCCTTT+TGG + chr5_3:54540180-54540199 Msa0818590:intron 45.0%
AGCCAAAAGGGTAACTAGCT+AGG - chr5_3:54540185-54540204 None:intergenic 45.0%
!! TTGGGTTGGAGAAGAATGCT+TGG + chr5_3:54540333-54540352 Msa0818590:CDS 45.0%
AAGAAGCTGACCAATCGAAC+AGG + chr5_3:54540523-54540542 Msa0818590:CDS 45.0%
AGAAGCTGACCAATCGAACA+GGG + chr5_3:54540524-54540543 Msa0818590:CDS 45.0%
!! ATTTTAGCAGCGTGGTCATC+GGG - chr5_3:54540627-54540646 None:intergenic 45.0%
!! GATTTTAGCAGCGTGGTCAT+CGG - chr5_3:54540628-54540647 None:intergenic 45.0%
TGACCACGCTGCTAAAATCT+CGG + chr5_3:54540629-54540648 Msa0818590:three_prime_UTR 45.0%
!! AATCCGAGATTTTAGCAGCG+TGG - chr5_3:54540635-54540654 None:intergenic 45.0%
GGACAAACCTCTCAAGGAAA+TGG + chr5_3:54540849-54540868 Msa0818590:three_prime_UTR 45.0%
CAAACCTCTCAAGGAAATGG+TGG + chr5_3:54540852-54540871 Msa0818590:three_prime_UTR 45.0%
CTGAGCCTTGAACAACTGCA+TGG - chr5_3:54538542-54538561 None:intergenic 50.0%
!! TTGGTTTGGCAAGCCTCAAG+TGG + chr5_3:54538572-54538591 Msa0818590:intron 50.0%
TGGATCATGGACAATGTGGG+TGG - chr5_3:54539091-54539110 None:intergenic 50.0%
! ACGAAGCGTGGACTGAAACT+AGG + chr5_3:54539344-54539363 Msa0818590:intron 50.0%
AACTAGGAAAGCCGAGTGTG+CGG + chr5_3:54539360-54539379 Msa0818590:intron 50.0%
TAGCCCCCATTCAAAACCAC+TGG + chr5_3:54539409-54539428 Msa0818590:intron 50.0%
!! AGCTGCATCGATCTTTTCCC+TGG - chr5_3:54539816-54539835 None:intergenic 50.0%
GTTGATCGCTGTCATTGGGT+TGG + chr5_3:54540319-54540338 Msa0818590:CDS 50.0%
GTCTTGGTTCCCTGTTCGAT+TGG - chr5_3:54540536-54540555 None:intergenic 50.0%
GGATCCACCATTTCCTTGAG+AGG - chr5_3:54540859-54540878 None:intergenic 50.0%
GTCGGTCTCTTCTCCGAATT+GGG - chr5_3:54540953-54540972 None:intergenic 50.0%
AGTCGGTCTCTTCTCCGAAT+TGG - chr5_3:54540954-54540973 None:intergenic 50.0%
TCGACCCTTTCCACCGTTGA+AGG - chr5_3:54538364-54538383 None:intergenic 55.0%
CAACGGTGGAAAGGGTCGAA+TGG + chr5_3:54538365-54538384 Msa0818590:CDS 55.0%
AGAGTGCACCGTTGAATCGG+CGG - chr5_3:54539387-54539406 None:intergenic 55.0%
! AGTGCGAGTTGAGTGACCAG+TGG - chr5_3:54539428-54539447 None:intergenic 55.0%
AGCGTGGTCATCGGGTTATG+TGG - chr5_3:54540619-54540638 None:intergenic 55.0%
!! GTGTAGCTGCAGAGGCACTG+AGG - chr5_3:54538773-54538792 None:intergenic 60.0%
GAAGGGTCGTGTAGCTGCAG+AGG - chr5_3:54538781-54538800 None:intergenic 60.0%
!! TGTGCGGACCGCCGATTCAA+CGG + chr5_3:54539376-54539395 Msa0818590:intron 60.0%
GAATCGGCGGTCCGCACACT+CGG - chr5_3:54539374-54539393 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 54538296 54541055 54538296 ID=Msa0818590;Name=Msa0818590
chr5_3 mRNA 54538296 54541055 54538296 ID=Msa0818590-mRNA-1;Parent=Msa0818590;Name=Msa0818590-mRNA-1;_AED=0.29;_eAED=0.29;_QI=60|0.25|0.4|0.8|0|0.4|5|453|122
chr5_3 exon 54538296 54538386 54538296 ID=Msa0818590-mRNA-1:exon:16536;Parent=Msa0818590-mRNA-1
chr5_3 exon 54538755 54538825 54538755 ID=Msa0818590-mRNA-1:exon:16537;Parent=Msa0818590-mRNA-1
chr5_3 exon 54539644 54539704 54539644 ID=Msa0818590-mRNA-1:exon:16538;Parent=Msa0818590-mRNA-1
chr5_3 exon 54540236 54540358 54540236 ID=Msa0818590-mRNA-1:exon:16539;Parent=Msa0818590-mRNA-1
chr5_3 exon 54540520 54541055 54540520 ID=Msa0818590-mRNA-1:exon:16540;Parent=Msa0818590-mRNA-1
chr5_3 five_prime_UTR 54538296 54538355 54538296 ID=Msa0818590-mRNA-1:five_prime_utr;Parent=Msa0818590-mRNA-1
chr5_3 CDS 54538356 54538386 54538356 ID=Msa0818590-mRNA-1:cds;Parent=Msa0818590-mRNA-1
chr5_3 CDS 54538755 54538825 54538755 ID=Msa0818590-mRNA-1:cds;Parent=Msa0818590-mRNA-1
chr5_3 CDS 54539644 54539704 54539644 ID=Msa0818590-mRNA-1:cds;Parent=Msa0818590-mRNA-1
chr5_3 CDS 54540236 54540358 54540236 ID=Msa0818590-mRNA-1:cds;Parent=Msa0818590-mRNA-1
chr5_3 CDS 54540520 54540602 54540520 ID=Msa0818590-mRNA-1:cds;Parent=Msa0818590-mRNA-1
chr5_3 three_prime_UTR 54540603 54541055 54540603 ID=Msa0818590-mRNA-1:three_prime_utr;Parent=Msa0818590-mRNA-1
Gene Sequence

>Msa0818590

ATGATCCTTCAACGGTGGAAAGGGTCGAATGGGTTCAAGTGCTATGCCTCAGTGCCTCTGCAGCTACACGACCCTTCCGTTATATATGCTCTGGTTACACAGTTCTTTAATTTCAAAAGATCTGAGACCGGTACTAATAAAGCATTTTTCACTTATTCATTAGGTAATACTCTTTTGAAGTTGAATCAGAGAATAACTAGTGATCCTTTTGATGATTTGTCAAATTGGGTTGATCATGTAGTTTCAGTTGATCGCTGTCATTGGGTTGGAGAAGAATGCTTGGATATAAAAGAAGCTGACCAATCGAACAGGGAACCAAGACTTAGAATTTGCAATGGGAATGGCTTACTGCCTACAACATATGCATGA
Protein sequence

>Msa0818590

MILQRWKGSNGFKCYASVPLQLHDPSVIYALVTQFFNFKRSETGTNKAFFTYSLGNTLLKLNQRITSDPFDDLSNWVDHVVSVDRCHWVGEECLDIKEADQSNREPRLRICNGNGLLPTTYA*