AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0726140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0726140 MtrunA17_Chr4g0002051 45.946 222 95 3 82 281 83 301 1.40e-57 187
Msa0726140 MtrunA17_Chr8g0381471 44.025 159 65 2 145 281 1 157 2.80e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTATCGAAAGCGATTTA+TGG 0.217435 5_1:+26647735 Msa0726140:CDS
AACCAATATTTAGGATAAAA+TGG 0.269706 5_1:-26647565 None:intergenic
GGTGGTTTCAAATCAACTTA+TGG 0.292463 5_1:+26648132 Msa0726140:CDS
ATTGTTTCAGGTAATTGATT+TGG 0.299049 5_1:+26647504 Msa0726140:intron
AATTGCTCATGTCGAAAGTT+TGG 0.300899 5_1:+26646896 Msa0726140:CDS
TAGGAATGATTGTGCGATTT+GGG 0.304560 5_1:+26648324 Msa0726140:intron
TTTCATGGAAAACTTGTTGA+TGG 0.314872 5_1:+26648092 Msa0726140:CDS
ATCAAATGGATGATGGAAAA+AGG 0.314997 5_1:+26648349 Msa0726140:CDS
GTCGGAATAAACCAATATTT+AGG 0.320561 5_1:-26647574 None:intergenic
CTTGATACTTGTCTTCCTCT+TGG 0.328648 5_1:-26647972 None:intergenic
TAATTAATCTTAATTGTTGT+AGG 0.331207 5_1:+26647302 Msa0726140:CDS
TTAGGAATGATTGTGCGATT+TGG 0.345924 5_1:+26648323 Msa0726140:intron
GAAGACAAGTATCAAGAAAA+TGG 0.346177 5_1:+26647979 Msa0726140:CDS
GGTTATCGAAAGCGATTTAT+GGG 0.356200 5_1:+26647736 Msa0726140:CDS
TTGATACTTGTCTTCCTCTT+GGG 0.380492 5_1:-26647971 None:intergenic
GGATTTGATCTTGCGGAATT+CGG 0.384621 5_1:+26648548 Msa0726140:CDS
ATCATCGTCAATAAATTTGT+TGG 0.391722 5_1:-26647395 None:intergenic
GTTTATTCCGACCACATTAT+CGG 0.391757 5_1:+26647585 Msa0726140:CDS
ACAATCTGTAGATCAAATAA+AGG 0.392762 5_1:+26646836 Msa0726140:CDS
TGAGCAATTTGTGAGCGTTT+TGG 0.431058 5_1:-26646882 None:intergenic
TCATCGTCAATAAATTTGTT+GGG 0.434932 5_1:-26647394 None:intergenic
TTAGCAACTCGTGTTGGCAT+TGG 0.451963 5_1:+26647687 Msa0726140:intron
GTGCGATTTGGGTAATCAAA+TGG 0.465432 5_1:+26648335 Msa0726140:CDS
ACAGAGATAGATTACGTTGA+AGG 0.486255 5_1:+26647343 Msa0726140:CDS
ATGGATCCCTCTTTCTATGT+GGG 0.495254 5_1:+26648111 Msa0726140:CDS
TTTGTTGCTCCACGTGGTTA+CGG 0.500598 5_1:+26648177 Msa0726140:CDS
GATGGATCCCTCTTTCTATG+TGG 0.506410 5_1:+26648110 Msa0726140:CDS
AGGAACTAGGAAGTTGCTGC+AGG 0.508385 5_1:+26647363 Msa0726140:CDS
TTGGGTAATCAAATGGATGA+TGG 0.515751 5_1:+26648342 Msa0726140:CDS
GATCCCTCTTTCTATGTGGG+TGG 0.525374 5_1:+26648114 Msa0726140:CDS
GAAACCACCCACATAGAAAG+AGG 0.536877 5_1:-26648118 None:intergenic
GAGTACTGAGTTTATTTCAA+AGG 0.549217 5_1:+26647762 Msa0726140:CDS
TAGATTACGTTGAAGGAACT+AGG 0.550308 5_1:+26647350 Msa0726140:CDS
TCTTAATTGTTGTAGGCAAG+AGG 0.559172 5_1:+26647309 Msa0726140:CDS
TTACACTAACAGGTGGATGA+AGG 0.560129 5_1:+26648471 Msa0726140:intron
CCACGTGGTTACGGAAGTCA+AGG 0.569221 5_1:+26648186 Msa0726140:CDS
ATCGAAAGCGATTTATGGGG+GGG 0.572674 5_1:+26647740 Msa0726140:CDS
TTAATAATTTACCGATAATG+TGG 0.589365 5_1:-26647596 None:intergenic
ATTTATTTAGCAACTCGTGT+TGG 0.592550 5_1:+26647681 Msa0726140:intron
TTATCGAAAGCGATTTATGG+GGG 0.592691 5_1:+26647738 Msa0726140:CDS
TAATTTACCGATAATGTGGT+CGG 0.599623 5_1:-26647592 None:intergenic
TATCGAAAGCGATTTATGGG+GGG 0.620517 5_1:+26647739 Msa0726140:CDS
AAAAGAGGGATTTGATCTTG+CGG 0.622365 5_1:+26648541 Msa0726140:CDS
GGAACTAGGAAGTTGCTGCA+GGG 0.630697 5_1:+26647364 Msa0726140:CDS
GTTATCGAAAGCGATTTATG+GGG 0.643499 5_1:+26647737 Msa0726140:CDS
TTTAAGTTTGTTGCTCCACG+TGG 0.650344 5_1:+26648171 Msa0726140:CDS
GTTCGTCAGCTCTTCCCAAG+AGG 0.652918 5_1:+26647957 Msa0726140:CDS
AAACCACCCACATAGAAAGA+GGG 0.662397 5_1:-26648117 None:intergenic
CCTTGACTTCCGTAACCACG+TGG 0.696038 5_1:-26648186 None:intergenic
CACGTGGTTACGGAAGTCAA+GGG 0.703622 5_1:+26648187 Msa0726140:CDS
TATGAGAAAGAACATAGACA+TGG 0.713561 5_1:+26646957 Msa0726140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACTCATAATTTTTTATTT+AGG + chr5_1:26648305-26648324 Msa0726140:intron 10.0%
!! TAATTAATCTTAATTGTTGT+AGG + chr5_1:26647302-26647321 Msa0726140:CDS 15.0%
!! CTACAATTATATGAAGAATT+TGG - chr5_1:26647147-26647166 None:intergenic 20.0%
!! ATTTCTCTTTAAATTGTTTC+AGG + chr5_1:26647492-26647511 Msa0726140:intron 20.0%
!!! AACCAATATTTAGGATAAAA+TGG - chr5_1:26647568-26647587 None:intergenic 20.0%
!! TTAATAATTTACCGATAATG+TGG - chr5_1:26647599-26647618 None:intergenic 20.0%
!!! TTTTTTATATCTTACGAGTT+TGG - chr5_1:26648213-26648232 None:intergenic 20.0%
! ACAATCTGTAGATCAAATAA+AGG + chr5_1:26646836-26646855 Msa0726140:CDS 25.0%
!! GTATCTAATTTGTAACCTAA+AGG - chr5_1:26647065-26647084 None:intergenic 25.0%
! TACAAATTAGATACCTCAAA+AGG + chr5_1:26647071-26647090 Msa0726140:intron 25.0%
! AAGAATTTGGTTAATACCTT+TGG - chr5_1:26647134-26647153 None:intergenic 25.0%
! TTATATGTTTGTTTATGCAG+TGG + chr5_1:26647193-26647212 Msa0726140:intron 25.0%
!! TCATCGTCAATAAATTTGTT+GGG - chr5_1:26647397-26647416 None:intergenic 25.0%
! ATCATCGTCAATAAATTTGT+TGG - chr5_1:26647398-26647417 None:intergenic 25.0%
!! ATTGTTTCAGGTAATTGATT+TGG + chr5_1:26647504-26647523 Msa0726140:intron 25.0%
!! CACCATTTTATCCTAAATAT+TGG + chr5_1:26647563-26647582 Msa0726140:CDS 25.0%
! ACCAACAGAAAAAATGATAA+AGG + chr5_1:26647714-26647733 Msa0726140:CDS 25.0%
! CCAACAGAAAAAATGATAAA+GGG + chr5_1:26647715-26647734 Msa0726140:CDS 25.0%
! CATAGCTATGTAGATAAAAA+GGG - chr5_1:26647821-26647840 None:intergenic 25.0%
! TCATAGCTATGTAGATAAAA+AGG - chr5_1:26647822-26647841 None:intergenic 25.0%
!!! ACATTTTTAGTCTCTTTTCA+TGG + chr5_1:26648077-26648096 Msa0726140:intron 25.0%
!! AAATCCCTCTTTTTATTGTA+AGG - chr5_1:26648534-26648553 None:intergenic 25.0%
!!! AATTTTGCGAAAGTTTGCTA+AGG + chr5_1:26646923-26646942 Msa0726140:CDS 30.0%
TATGAGAAAGAACATAGACA+TGG + chr5_1:26646957-26646976 Msa0726140:CDS 30.0%
AGGAAACTCTTCTCAATTAA+AGG + chr5_1:26647008-26647027 Msa0726140:intron 30.0%
!!! GGTGTTTTGGTTACAATAAT+CGG - chr5_1:26647044-26647063 None:intergenic 30.0%
ATTGTAACCAAAACACCTTT+AGG + chr5_1:26647047-26647066 Msa0726140:intron 30.0%
!!! TTTGTAACCTAAAGGTGTTT+TGG - chr5_1:26647057-26647076 None:intergenic 30.0%
AATTGCAGCTAATATTCCAA+AGG + chr5_1:26647115-26647134 Msa0726140:intron 30.0%
GTCGGAATAAACCAATATTT+AGG - chr5_1:26647577-26647596 None:intergenic 30.0%
TAATTTACCGATAATGTGGT+CGG - chr5_1:26647595-26647614 None:intergenic 30.0%
ATTTATTTAGCAACTCGTGT+TGG + chr5_1:26647681-26647700 Msa0726140:intron 30.0%
!!! CCCTTTATCATTTTTTCTGT+TGG - chr5_1:26647718-26647737 None:intergenic 30.0%
!! GAGTACTGAGTTTATTTCAA+AGG + chr5_1:26647762-26647781 Msa0726140:CDS 30.0%
TTCCTTCTCATAATTGACAT+CGG + chr5_1:26647899-26647918 Msa0726140:intron 30.0%
GAAGACAAGTATCAAGAAAA+TGG + chr5_1:26647979-26647998 Msa0726140:CDS 30.0%
!! TTTCATGGAAAACTTGTTGA+TGG + chr5_1:26648092-26648111 Msa0726140:CDS 30.0%
!! ATCAAATGGATGATGGAAAA+AGG + chr5_1:26648349-26648368 Msa0726140:CDS 30.0%
TTTACCACAATTACACTAAC+AGG + chr5_1:26648461-26648480 Msa0726140:intron 30.0%
TGCATCCTTACAATAAAAAG+AGG + chr5_1:26648526-26648545 Msa0726140:CDS 30.0%
GCATCCTTACAATAAAAAGA+GGG + chr5_1:26648527-26648546 Msa0726140:CDS 30.0%
AATTGCTCATGTCGAAAGTT+TGG + chr5_1:26646896-26646915 Msa0726140:CDS 35.0%
TTTGGTTACAATAATCGGAG+TGG - chr5_1:26647039-26647058 None:intergenic 35.0%
ATTAGATACCTCAAAAGGCA+AGG + chr5_1:26647076-26647095 Msa0726140:intron 35.0%
TCTTAATTGTTGTAGGCAAG+AGG + chr5_1:26647309-26647328 Msa0726140:CDS 35.0%
ACAGAGATAGATTACGTTGA+AGG + chr5_1:26647343-26647362 Msa0726140:CDS 35.0%
TAGATTACGTTGAAGGAACT+AGG + chr5_1:26647350-26647369 Msa0726140:CDS 35.0%
GTTTATTCCGACCACATTAT+CGG + chr5_1:26647585-26647604 Msa0726140:CDS 35.0%
GGTTATCGAAAGCGATTTAT+GGG + chr5_1:26647736-26647755 Msa0726140:CDS 35.0%
GTTATCGAAAGCGATTTATG+GGG + chr5_1:26647737-26647756 Msa0726140:CDS 35.0%
TTATCGAAAGCGATTTATGG+GGG + chr5_1:26647738-26647757 Msa0726140:CDS 35.0%
CAATGAACGGTATCATGTAT+CGG - chr5_1:26647878-26647897 None:intergenic 35.0%
TATGAGAAGGAACCAATGAA+CGG - chr5_1:26647891-26647910 None:intergenic 35.0%
TTGATACTTGTCTTCCTCTT+GGG - chr5_1:26647974-26647993 None:intergenic 35.0%
GGTGGTTTCAAATCAACTTA+TGG + chr5_1:26648132-26648151 Msa0726140:CDS 35.0%
TTAGGAATGATTGTGCGATT+TGG + chr5_1:26648323-26648342 Msa0726140:intron 35.0%
TAGGAATGATTGTGCGATTT+GGG + chr5_1:26648324-26648343 Msa0726140:intron 35.0%
TTGGGTAATCAAATGGATGA+TGG + chr5_1:26648342-26648361 Msa0726140:CDS 35.0%
AAAAGAGGGATTTGATCTTG+CGG + chr5_1:26648541-26648560 Msa0726140:CDS 35.0%
! TGAGCAATTTGTGAGCGTTT+TGG - chr5_1:26646885-26646904 None:intergenic 40.0%
CTGCACAAAATCACAGTGTT+AGG + chr5_1:26646988-26647007 Msa0726140:intron 40.0%
!! GTTGACTTTTGATGAGAGTC+TGG + chr5_1:26647537-26647556 Msa0726140:CDS 40.0%
GGGTTATCGAAAGCGATTTA+TGG + chr5_1:26647735-26647754 Msa0726140:CDS 40.0%
TATCGAAAGCGATTTATGGG+GGG + chr5_1:26647739-26647758 Msa0726140:CDS 40.0%
CGATACATGATACCGTTCAT+TGG + chr5_1:26647876-26647895 Msa0726140:intron 40.0%
TGCCGATGTCAATTATGAGA+AGG - chr5_1:26647904-26647923 None:intergenic 40.0%
CATAATTGACATCGGCAGAA+AGG + chr5_1:26647907-26647926 Msa0726140:intron 40.0%
CTTGATACTTGTCTTCCTCT+TGG - chr5_1:26647975-26647994 None:intergenic 40.0%
ATGGATCCCTCTTTCTATGT+GGG + chr5_1:26648111-26648130 Msa0726140:CDS 40.0%
AAACCACCCACATAGAAAGA+GGG - chr5_1:26648120-26648139 None:intergenic 40.0%
!! TTTAAGTTTGTTGCTCCACG+TGG + chr5_1:26648171-26648190 Msa0726140:CDS 40.0%
! GTGCGATTTGGGTAATCAAA+TGG + chr5_1:26648335-26648354 Msa0726140:CDS 40.0%
ACCACAATTACACTAACAGG+TGG + chr5_1:26648464-26648483 Msa0726140:intron 40.0%
TCCACCTGTTAGTGTAATTG+TGG - chr5_1:26648468-26648487 None:intergenic 40.0%
TTACACTAACAGGTGGATGA+AGG + chr5_1:26648471-26648490 Msa0726140:intron 40.0%
GGATTTGATCTTGCGGAATT+CGG + chr5_1:26648548-26648567 Msa0726140:CDS 40.0%
! TTAGCAACTCGTGTTGGCAT+TGG + chr5_1:26647687-26647706 Msa0726140:intron 45.0%
ATCGAAAGCGATTTATGGGG+GGG + chr5_1:26647740-26647759 Msa0726140:CDS 45.0%
TGACATCGGCAGAAAGGAAT+TGG + chr5_1:26647913-26647932 Msa0726140:intron 45.0%
GATGGATCCCTCTTTCTATG+TGG + chr5_1:26648110-26648129 Msa0726140:CDS 45.0%
GAAACCACCCACATAGAAAG+AGG - chr5_1:26648121-26648140 None:intergenic 45.0%
TTTGTTGCTCCACGTGGTTA+CGG + chr5_1:26648177-26648196 Msa0726140:CDS 45.0%
!!! TAAATATTATCTTATTTTAT+AGG + chr5_1:26647262-26647281 Msa0726140:intron 5.0%
! GCGAGATTCCTTGCCTTTTG+AGG - chr5_1:26647087-26647106 None:intergenic 50.0%
AGGAACTAGGAAGTTGCTGC+AGG + chr5_1:26647363-26647382 Msa0726140:CDS 50.0%
GGAACTAGGAAGTTGCTGCA+GGG + chr5_1:26647364-26647383 Msa0726140:CDS 50.0%
GATCCCTCTTTCTATGTGGG+TGG + chr5_1:26648114-26648133 Msa0726140:CDS 50.0%
CACGTGGTTACGGAAGTCAA+GGG + chr5_1:26648187-26648206 Msa0726140:CDS 50.0%
GTTCGTCAGCTCTTCCCAAG+AGG + chr5_1:26647957-26647976 Msa0726140:CDS 55.0%
CCTTGACTTCCGTAACCACG+TGG - chr5_1:26648189-26648208 None:intergenic 55.0%
CCACGTGGTTACGGAAGTCA+AGG + chr5_1:26648186-26648205 Msa0726140:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 26646807 26648584 26646807 ID=Msa0726140;Name=Msa0726140
chr5_1 mRNA 26646807 26648584 26646807 ID=Msa0726140-mRNA-1;Parent=Msa0726140;Name=Msa0726140-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0|0|0.5|0.71|0.75|8|0|295
chr5_1 exon 26646807 26647002 26646807 ID=Msa0726140-mRNA-1:exon:9449;Parent=Msa0726140-mRNA-1
chr5_1 exon 26647284 26647417 26647284 ID=Msa0726140-mRNA-1:exon:9450;Parent=Msa0726140-mRNA-1
chr5_1 exon 26647514 26647606 26647514 ID=Msa0726140-mRNA-1:exon:9451;Parent=Msa0726140-mRNA-1
chr5_1 exon 26647691 26647783 26647691 ID=Msa0726140-mRNA-1:exon:9452;Parent=Msa0726140-mRNA-1
chr5_1 exon 26647929 26648000 26647929 ID=Msa0726140-mRNA-1:exon:9453;Parent=Msa0726140-mRNA-1
chr5_1 exon 26648087 26648217 26648087 ID=Msa0726140-mRNA-1:exon:9454;Parent=Msa0726140-mRNA-1
chr5_1 exon 26648327 26648393 26648327 ID=Msa0726140-mRNA-1:exon:9455;Parent=Msa0726140-mRNA-1
chr5_1 exon 26648483 26648584 26648483 ID=Msa0726140-mRNA-1:exon:9456;Parent=Msa0726140-mRNA-1
chr5_1 CDS 26646807 26647002 26646807 ID=Msa0726140-mRNA-1:cds;Parent=Msa0726140-mRNA-1
chr5_1 CDS 26647284 26647417 26647284 ID=Msa0726140-mRNA-1:cds;Parent=Msa0726140-mRNA-1
chr5_1 CDS 26647514 26647606 26647514 ID=Msa0726140-mRNA-1:cds;Parent=Msa0726140-mRNA-1
chr5_1 CDS 26647691 26647783 26647691 ID=Msa0726140-mRNA-1:cds;Parent=Msa0726140-mRNA-1
chr5_1 CDS 26647929 26648000 26647929 ID=Msa0726140-mRNA-1:cds;Parent=Msa0726140-mRNA-1
chr5_1 CDS 26648087 26648217 26648087 ID=Msa0726140-mRNA-1:cds;Parent=Msa0726140-mRNA-1
chr5_1 CDS 26648327 26648393 26648327 ID=Msa0726140-mRNA-1:cds;Parent=Msa0726140-mRNA-1
chr5_1 CDS 26648483 26648584 26648483 ID=Msa0726140-mRNA-1:cds;Parent=Msa0726140-mRNA-1
Gene Sequence

>Msa0726140

ATGGTAGAGACAGTTGTTTACAGCACAAAACAATCTGTAGATCAAATAAAGGAAATACTTGTGAAGAGCGCTACACCAAAACGCTCACAAATTGCTCATGTCGAAAGTTTGGAAGAAATTTTGCGAAAGTTTGCTAAGGAAAAAGATTCTTATGAGAAAGAACATAGACATGGCAAAGAATCTGCACAAAATCACAGTTGTACATATAATGATATAATTAATCTTAATTGTTGTAGGCAAGAGGTCATGATCAGTACAGAGATAGATTACGTTGAAGGAACTAGGAAGTTGCTGCAGGGTTTGAAACCCAACAAATTTATTGACGATGATGTAATTGATTTGGCTGCTTGCATGTTGACTTTTGATGAGAGTCTGGAATCACCATTTTATCCTAAATATTGGTTTATTCCGACCACATTATCGCAACTCGTGTTGGCATTGGAAACACCAACAGAAAAAATGATAAAGGGTTATCGAAAGCGATTTATGGGGGGGAGTACTGAGTTTATTTCAAAGGAATTGGTTTATCTTGACTCGTATCAAAGTTCGTCAGCTCTTCCCAAGAGGAAGACAAGTATCAAGAAAATGTCTCTTTTCATGGAAAACTTGTTGATGGATCCCTCTTTCTATGTGGGTGGTTTCAAATCAACTTATGGACACAAGATATCAGAGTTTAAGTTTGTTGCTCCACGTGGTTACGGAAGTCAAGGGCCAAACTCGAATGATTGTGCGATTTGGGTAATCAAATGGATGATGGAAAAAGGAGCTGATGAATATAAGATTAAAGTGGATGAAGGCTCAAGACTACAAACTGCACTGCAATTGACAATGCATCCTTACAATAAAAAGAGGGATTTGATCTTGCGGAATTCGGTGAAATACAGATAA

Protein sequence

>Msa0726140

MVETVVYSTKQSVDQIKEILVKSATPKRSQIAHVESLEEILRKFAKEKDSYEKEHRHGKESAQNHSCTYNDIINLNCCRQEVMISTEIDYVEGTRKLLQGLKPNKFIDDDVIDLAACMLTFDESLESPFYPKYWFIPTTLSQLVLALETPTEKMIKGYRKRFMGGSTEFISKELVYLDSYQSSSALPKRKTSIKKMSLFMENLLMDPSFYVGGFKSTYGHKISEFKFVAPRGYGSQGPNSNDCAIWVIKWMMEKGADEYKIKVDEGSRLQTALQLTMHPYNKKRDLILRNSVKYR*