Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821470 | AES99104.2 | 96.667 | 60 | 2 | 0 | 8 | 67 | 1 | 60 | 3.11e-34 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821470 | G7KFR7 | 96.667 | 60 | 2 | 0 | 8 | 67 | 1 | 60 | 1.49e-34 | 120 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0736320 | Msa0821470 | 0.834156 | 3.526711e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821470 | MtrunA17_Chr5g0433571 | 96.667 | 60 | 2 | 0 | 8 | 67 | 1 | 60 | 2.86e-38 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGGCCATGGACATTA+AGG | 0.344635 | 5_3:-59616894 | None:intergenic |
| GGAATGACGTGGTGGAAAAT+AGG | 0.353143 | 5_3:-59616909 | None:intergenic |
| ACCTTCTGAGGAACAATTGA+AGG | 0.461328 | 5_3:+59616836 | Msa0821470:CDS |
| ATGAACTAGTGGAGATAGCC+AGG | 0.504297 | 5_3:-59616930 | None:intergenic |
| GTCGTTCACCTTAATGTCCA+TGG | 0.510017 | 5_3:+59616886 | Msa0821470:exon |
| TATAATTTGTCTTGGAAACA+CGG | 0.534269 | 5_3:-59616606 | None:intergenic |
| GCCTTCAATTGTTCCTCAGA+AGG | 0.544332 | 5_3:-59616837 | None:intergenic |
| TATCTATGACTATGAACTAG+TGG | 0.548108 | 5_3:-59616941 | None:intergenic |
| CTCACTCATACTATGCTTCG+TGG | 0.556047 | 5_3:-59616525 | None:intergenic |
| TCTTGAAGAGTACATCAAAT+CGG | 0.557421 | 5_3:+59616860 | Msa0821470:CDS |
| CAATGAAACACCACCTTCTG+AGG | 0.567138 | 5_3:+59616824 | Msa0821470:CDS |
| TTCAATTGTTCCTCAGAAGG+TGG | 0.592646 | 5_3:-59616834 | None:intergenic |
| ACGTGGTGGAAAATAGGCCA+TGG | 0.603725 | 5_3:-59616903 | None:intergenic |
| GGAGATAGCCAGGAATGACG+TGG | 0.699259 | 5_3:-59616920 | None:intergenic |
| GATAGCCAGGAATGACGTGG+TGG | 0.711922 | 5_3:-59616917 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAATATGATAAATATATAGT+AGG | - | chr5_3:59616665-59616684 | None:intergenic | 15.0% |
| !!! | AAAGATCTTCATCTTTTTAT+TGG | - | chr5_3:59616579-59616598 | None:intergenic | 20.0% |
| !! | AATAAAAAGATGAAGATCTT+TGG | + | chr5_3:59616578-59616597 | Msa0821470:CDS | 20.0% |
| !!! | AAATTATACTTTTTGCATCA+GGG | + | chr5_3:59616621-59616640 | Msa0821470:CDS | 20.0% |
| !! | GATAAATATATAGTAGGATT+TGG | - | chr5_3:59616659-59616678 | None:intergenic | 20.0% |
| !! | ACTGTAAATTAAACAATAAC+AGG | + | chr5_3:59616751-59616770 | Msa0821470:intron | 20.0% |
| !!! | AATATGACACTTATTTTAGA+GGG | - | chr5_3:59616972-59616991 | None:intergenic | 20.0% |
| !!! | AAATATGACACTTATTTTAG+AGG | - | chr5_3:59616973-59616992 | None:intergenic | 20.0% |
| !! | TATAATTTGTCTTGGAAACA+CGG | - | chr5_3:59616609-59616628 | None:intergenic | 25.0% |
| !! | GCAAAAAGTATAATTTGTCT+TGG | - | chr5_3:59616617-59616636 | None:intergenic | 25.0% |
| !!! | CAAATTATACTTTTTGCATC+AGG | + | chr5_3:59616620-59616639 | Msa0821470:CDS | 25.0% |
| ! | TCATCATCTTATGTTATCTT+AGG | + | chr5_3:59616683-59616702 | Msa0821470:intron | 25.0% |
| ! | CATCATCTTATGTTATCTTA+GGG | + | chr5_3:59616684-59616703 | Msa0821470:intron | 25.0% |
| ! | GTTAATCACAACAAAATCAT+TGG | - | chr5_3:59616733-59616752 | None:intergenic | 25.0% |
| ! | TCTTGAAGAGTACATCAAAT+CGG | + | chr5_3:59616860-59616879 | Msa0821470:CDS | 30.0% |
| TATCTATGACTATGAACTAG+TGG | - | chr5_3:59616944-59616963 | None:intergenic | 30.0% | |
| ! | CAATAACAGGCTTTTGTTCT+TGG | + | chr5_3:59616764-59616783 | Msa0821470:intron | 35.0% |
| AAAATAGGCCATGGACATTA+AGG | - | chr5_3:59616897-59616916 | None:intergenic | 35.0% | |
| !!! | ATGACACTTATTTTAGAGGG+AGG | - | chr5_3:59616969-59616988 | None:intergenic | 35.0% |
| TTCAATTGTTCCTCAGAAGG+TGG | - | chr5_3:59616837-59616856 | None:intergenic | 40.0% | |
| ! | ACCTTCTGAGGAACAATTGA+AGG | + | chr5_3:59616836-59616855 | Msa0821470:CDS | 40.0% |
| CTCACTCATACTATGCTTCG+TGG | - | chr5_3:59616528-59616547 | None:intergenic | 45.0% | |
| CAATGAAACACCACCTTCTG+AGG | + | chr5_3:59616824-59616843 | Msa0821470:CDS | 45.0% | |
| GCCTTCAATTGTTCCTCAGA+AGG | - | chr5_3:59616840-59616859 | None:intergenic | 45.0% | |
| GTCGTTCACCTTAATGTCCA+TGG | + | chr5_3:59616886-59616905 | Msa0821470:exon | 45.0% | |
| ! | GGAATGACGTGGTGGAAAAT+AGG | - | chr5_3:59616912-59616931 | None:intergenic | 45.0% |
| ! | ATTTTCCACCACGTCATTCC+TGG | + | chr5_3:59616912-59616931 | Msa0821470:three_prime_UTR | 45.0% |
| ATGAACTAGTGGAGATAGCC+AGG | - | chr5_3:59616933-59616952 | None:intergenic | 45.0% | |
| ! | ACGTGGTGGAAAATAGGCCA+TGG | - | chr5_3:59616906-59616925 | None:intergenic | 50.0% |
| GATAGCCAGGAATGACGTGG+TGG | - | chr5_3:59616920-59616939 | None:intergenic | 55.0% | |
| GGAGATAGCCAGGAATGACG+TGG | - | chr5_3:59616923-59616942 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 59616504 | 59617081 | 59616504 | ID=Msa0821470;Name=Msa0821470 |
| chr5_3 | mRNA | 59616504 | 59617081 | 59616504 | ID=Msa0821470-mRNA-1;Parent=Msa0821470;Name=Msa0821470-mRNA-1;_AED=0.03;_eAED=0.03;_QI=62|1|1|1|1|1|2|182|67 |
| chr5_3 | exon | 59616504 | 59616642 | 59616504 | ID=Msa0821470-mRNA-1:exon:18140;Parent=Msa0821470-mRNA-1 |
| chr5_3 | exon | 59616773 | 59617081 | 59616773 | ID=Msa0821470-mRNA-1:exon:18141;Parent=Msa0821470-mRNA-1 |
| chr5_3 | five_prime_UTR | 59616504 | 59616565 | 59616504 | ID=Msa0821470-mRNA-1:five_prime_utr;Parent=Msa0821470-mRNA-1 |
| chr5_3 | CDS | 59616566 | 59616642 | 59616566 | ID=Msa0821470-mRNA-1:cds;Parent=Msa0821470-mRNA-1 |
| chr5_3 | CDS | 59616773 | 59616899 | 59616773 | ID=Msa0821470-mRNA-1:cds;Parent=Msa0821470-mRNA-1 |
| chr5_3 | three_prime_UTR | 59616900 | 59617081 | 59616900 | ID=Msa0821470-mRNA-1:three_prime_utr;Parent=Msa0821470-mRNA-1 |
| Gene Sequence |
| Protein sequence |
