AlfalfaGEDB | Msa0736320

M. sativa cultivar zhongmu-4 / Msa0736320

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736320	AES99104.2	96.667	60	2	0	8	67	1	60	3.11e-34	120

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736320	G7KFR7	96.667	60	2	0	8	67	1	60	1.49e-34	120

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011170	Msa0736320	0.806921	6.505368e-50	-8.615850e-47
Msa0115270	Msa0736320	0.810179	1.311072e-50	-8.615850e-47
Msa0119850	Msa0736320	0.804835	1.785346e-49	-8.615850e-47
Msa0608710	Msa0736320	0.808631	2.816362e-50	-8.615850e-47
Msa0632870	Msa0736320	0.802238	6.168144e-49	-8.615850e-47
Msa0647450	Msa0736320	-0.815518	8.871006e-52	-8.615850e-47
Msa0661190	Msa0736320	-0.815862	7.436900e-52	-8.615850e-47
Msa0678820	Msa0736320	0.809359	1.967257e-50	-8.615850e-47
Msa0736320	Msa0743260	0.800036	1.740226e-48	-8.615850e-47
Msa0736320	Msa0743720	0.804946	1.692016e-49	-8.615850e-47
Msa0736320	Msa0761800	0.804645	1.956046e-49	-8.615850e-47
Msa0736320	Msa0771720	0.802371	5.792378e-49	-8.615850e-47
Msa0736320	Msa0812760	0.809399	1.929286e-50	-8.615850e-47
Msa0736320	Msa0821470	0.834156	3.526711e-56	-8.615850e-47
Msa0736320	Msa0824680	0.802038	6.780991e-49	-8.615850e-47
Msa0736320	Msa0863870	0.803781	2.959484e-49	-8.615850e-47
Msa0736320	Msa0935850	0.802165	6.386371e-49	-8.615850e-47
Msa0736320	Msa0992490	-0.838172	3.367815e-57	-8.615850e-47
Msa0736320	Msa1012830	0.820219	7.701033e-53	-8.615850e-47
Msa0736320	Msa1054410	0.806886	6.616665e-50	-8.615850e-47
Msa0736320	Msa1082500	0.800101	1.687726e-48	-8.615850e-47
Msa0736320	Msa1116130	-0.804632	1.968216e-49	-8.615850e-47
Msa0736320	Msa1233500	0.810636	1.044448e-50	-8.615850e-47
Msa0736320	Msa1233890	0.805017	1.635629e-49	-8.615850e-47
Msa0736320	Msa1279210	0.815102	1.097646e-51	-8.615850e-47
Msa0736320	Msa1300050	0.811825	5.769025e-51	-8.615850e-47
Msa0736320	Msa1348950	0.816905	4.345801e-52	-8.615850e-47
Msa0736320	Msa1362880	0.809849	1.544117e-50	-8.615850e-47
Msa0736320	Msa1444740	0.806651	7.418607e-50	-8.615850e-47
Msa0152170	Msa0736320	0.809419	1.910223e-50	-8.615850e-47
Msa0380040	Msa0736320	0.822479	2.317774e-53	-8.615850e-47
Msa0399340	Msa0736320	0.810076	1.379629e-50	-8.615850e-47
Msa0429290	Msa0736320	0.802906	4.492494e-49	-8.615850e-47
Msa0442200	Msa0736320	0.825569	4.365172e-54	-8.615850e-47
Msa0467900	Msa0736320	-0.821893	3.169413e-53	-8.615850e-47
Msa0475580	Msa0736320	0.817578	3.066561e-52	-8.615850e-47
Msa0477080	Msa0736320	0.802473	5.516571e-49	-8.615850e-47
Msa0344830	Msa0736320	0.813342	2.686847e-51	-8.615850e-47
Msa0519220	Msa0736320	0.814919	1.205523e-51	-8.615850e-47
Msa0540070	Msa0736320	0.817316	3.512207e-52	-8.615850e-47
Msa0543900	Msa0736320	0.804747	1.862112e-49	-8.615850e-47
Msa0562440	Msa0736320	0.822537	2.246380e-53	-8.615850e-47
Msa0599770	Msa0736320	0.818991	1.467847e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0736320	MtrunA17_Chr5g0433571	96.667	60	2	0	8	67	1	60	2.86e-38	120

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 18 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGGCCATGGACATTA+AGG	0.344635	5_1:-52453735	None:intergenic
GGAATGACGTGGTGGAAAAT+AGG	0.362736	5_1:-52453750	None:intergenic
AAACATGACACTTATTTAAG+AGG	0.442906	5_1:-52453811	None:intergenic
ACCTTCTGAGGAACAATTGA+AGG	0.461328	5_1:+52453677	Msa0736320:CDS
GTCGTTCACCTTAATGTCCA+TGG	0.510017	5_1:+52453727	Msa0736320:exon
ATGAACTAGTGGAGATAGCT+AGG	0.521615	5_1:-52453771	None:intergenic
AACATGACACTTATTTAAGA+GGG	0.527972	5_1:-52453810	None:intergenic
TATAATTTGTCTTGGAAACA+CGG	0.534269	5_1:-52453447	None:intergenic
GCCTTCAATTGTTCCTCAGA+AGG	0.544332	5_1:-52453678	None:intergenic
TATCTATGACTATGAACTAG+TGG	0.548108	5_1:-52453782	None:intergenic
CTCACTCATACTATGCTTCG+TGG	0.556047	5_1:-52453366	None:intergenic
TCTTGAAGAGTACATCAAAT+CGG	0.557421	5_1:+52453701	Msa0736320:CDS
CAATGAAACACCACCTTCTG+AGG	0.567138	5_1:+52453665	Msa0736320:CDS
TTCAATTGTTCCTCAGAAGG+TGG	0.592646	5_1:-52453675	None:intergenic
ACGTGGTGGAAAATAGGCCA+TGG	0.603725	5_1:-52453744	None:intergenic
ATGACACTTATTTAAGAGGG+AGG	0.627766	5_1:-52453807	None:intergenic
GATAGCTAGGAATGACGTGG+TGG	0.711922	5_1:-52453758	None:intergenic
GGAGATAGCTAGGAATGACG+TGG	0.729608	5_1:-52453761	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAATATGATAAATATATAGT+AGG	-	chr5_1:52453506-52453525	None:intergenic	15.0%
!!!	AAAGATCTTCATCTTTTTAT+TGG	-	chr5_1:52453420-52453439	None:intergenic	20.0%
!!	AATAAAAAGATGAAGATCTT+TGG	+	chr5_1:52453419-52453438	Msa0736320:CDS	20.0%
!!!	AAATTATACTTTTTGCATCA+GGG	+	chr5_1:52453462-52453481	Msa0736320:CDS	20.0%
!!	GATAAATATATAGTAGGATT+TGG	-	chr5_1:52453500-52453519	None:intergenic	20.0%
!!	ACTGTAAATTAAACAATAAC+AGG	+	chr5_1:52453592-52453611	Msa0736320:intron	20.0%
!!	TATAATTTGTCTTGGAAACA+CGG	-	chr5_1:52453450-52453469	None:intergenic	25.0%
!!	GCAAAAAGTATAATTTGTCT+TGG	-	chr5_1:52453458-52453477	None:intergenic	25.0%
!!!	CAAATTATACTTTTTGCATC+AGG	+	chr5_1:52453461-52453480	Msa0736320:CDS	25.0%
!	TCATCATCTTATGTTATCTT+AGG	+	chr5_1:52453524-52453543	Msa0736320:intron	25.0%
!	CATCATCTTATGTTATCTTA+GGG	+	chr5_1:52453525-52453544	Msa0736320:intron	25.0%
!	GTTAATCACAACAAAATCAT+TGG	-	chr5_1:52453574-52453593	None:intergenic	25.0%
!	AACATGACACTTATTTAAGA+GGG	-	chr5_1:52453813-52453832	None:intergenic	25.0%
!	AAACATGACACTTATTTAAG+AGG	-	chr5_1:52453814-52453833	None:intergenic	25.0%
!	TCTTGAAGAGTACATCAAAT+CGG	+	chr5_1:52453701-52453720	Msa0736320:CDS	30.0%
	TATCTATGACTATGAACTAG+TGG	-	chr5_1:52453785-52453804	None:intergenic	30.0%
!	CAATAACAGGCTTTTGTTCT+TGG	+	chr5_1:52453605-52453624	Msa0736320:intron	35.0%
	AAAATAGGCCATGGACATTA+AGG	-	chr5_1:52453738-52453757	None:intergenic	35.0%
	ATGACACTTATTTAAGAGGG+AGG	-	chr5_1:52453810-52453829	None:intergenic	35.0%
	TTCAATTGTTCCTCAGAAGG+TGG	-	chr5_1:52453678-52453697	None:intergenic	40.0%
!	ACCTTCTGAGGAACAATTGA+AGG	+	chr5_1:52453677-52453696	Msa0736320:CDS	40.0%
	ATGAACTAGTGGAGATAGCT+AGG	-	chr5_1:52453774-52453793	None:intergenic	40.0%
	CTCACTCATACTATGCTTCG+TGG	-	chr5_1:52453369-52453388	None:intergenic	45.0%
	CAATGAAACACCACCTTCTG+AGG	+	chr5_1:52453665-52453684	Msa0736320:CDS	45.0%
	GCCTTCAATTGTTCCTCAGA+AGG	-	chr5_1:52453681-52453700	None:intergenic	45.0%
	GTCGTTCACCTTAATGTCCA+TGG	+	chr5_1:52453727-52453746	Msa0736320:exon	45.0%
!	GGAATGACGTGGTGGAAAAT+AGG	-	chr5_1:52453753-52453772	None:intergenic	45.0%
!	ACGTGGTGGAAAATAGGCCA+TGG	-	chr5_1:52453747-52453766	None:intergenic	50.0%
	GATAGCTAGGAATGACGTGG+TGG	-	chr5_1:52453761-52453780	None:intergenic	50.0%
	GGAGATAGCTAGGAATGACG+TGG	-	chr5_1:52453764-52453783	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	52453345	52453921	52453345	ID=Msa0736320;Name=Msa0736320
chr5_1	mRNA	52453345	52453921	52453345	ID=Msa0736320-mRNA-1;Parent=Msa0736320;Name=Msa0736320-mRNA-1;_AED=0.03;_eAED=0.03;_QI=62\|1\|1\|1\|1\|1\|2\|181\|67
chr5_1	exon	52453345	52453483	52453345	ID=Msa0736320-mRNA-1:exon:14794;Parent=Msa0736320-mRNA-1
chr5_1	exon	52453614	52453921	52453614	ID=Msa0736320-mRNA-1:exon:14795;Parent=Msa0736320-mRNA-1
chr5_1	five_prime_UTR	52453345	52453406	52453345	ID=Msa0736320-mRNA-1:five_prime_utr;Parent=Msa0736320-mRNA-1
chr5_1	CDS	52453407	52453483	52453407	ID=Msa0736320-mRNA-1:cds;Parent=Msa0736320-mRNA-1
chr5_1	CDS	52453614	52453740	52453614	ID=Msa0736320-mRNA-1:cds;Parent=Msa0736320-mRNA-1
chr5_1	three_prime_UTR	52453741	52453921	52453741	ID=Msa0736320-mRNA-1:three_prime_utr;Parent=Msa0736320-mRNA-1

Gene Sequence	>Msa0736320 ATGAACAACACCAATAAAAAGATGAAGATCTTTGGCAAAACCGTGTTTCCAAGACAAATTATACTTTTTGCATCAGGGCTTTTGTTCTTGGCATCAACAACTTATGATGTTCATAGATCAATCAAGAACAATGAAACACCACCTTCTGAGGAACAATTGAAGGCTCTTGAAGAGTACATCAAATCGGTGCGTCGTTCACCTTAA
Protein sequence	>Msa0736320 MNNTNKKMKIFGKTVFPRQIILFASGLLFLASTTYDVHRSIKNNETPPSEEQLKALEEYIKSVRRSP*