AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0824440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0824440 MtrunA17_Chr5g0445201 93.171 205 13 1 1 205 1 204 1.91e-127 357
Msa0824440 MtrunA17_Chr2g0312891 49.254 67 34 0 78 144 96 162 6.41e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0824440 AT4G26670.1 65.327 199 65 3 5 199 11 209 1.31e-82 244
Msa0824440 AT5G55510.1 63.542 192 70 0 15 206 23 214 6.34e-82 243
Msa0824440 AT3G10110.1 43.284 67 38 0 78 144 100 166 8.51e-12 61.6

Find 83 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAGGGTTGCCTCACTTT+TGG 0.154502 5_3:-63697884 Msa0824440:CDS
TTCCGCCGGTGGCGCTTTAA+TGG 0.189872 5_3:-63700666 Msa0824440:CDS
TGCCACTACTGTTGCTGTTT+TGG 0.236153 5_3:+63700829 None:intergenic
CTGCCCATGTCCTGGAAAAC+AGG 0.282308 5_3:-63697809 Msa0824440:CDS
AAAGCTTCTTTCAATTCATT+TGG 0.319112 5_3:+63697734 None:intergenic
TTCTTAAATTATTCAGTGTT+TGG 0.319617 5_3:+63697355 None:intergenic
GATGGCGGTGTTGGGAGAAT+TGG 0.325669 5_3:+63700733 None:intergenic
TCCGCCGGTGGCGCTTTAAT+GGG 0.333280 5_3:-63700665 Msa0824440:CDS
TACTTCACATAAGTGTATTT+CGG 0.339634 5_3:+63697393 None:intergenic
GAATATATGTAATGATTGAT+TGG 0.359410 5_3:-63697438 Msa0824440:three_prime_UTR
GTCATTAACGCTGGAGTAGC+TGG 0.388658 5_3:-63699190 Msa0824440:CDS
GCGGGAACGATGGCGGTGTT+GGG 0.392409 5_3:+63700725 None:intergenic
CATTTGGAAGAGGGAGTTGA+AGG 0.400267 5_3:+63697750 None:intergenic
GCTGGATTGTTTAAGAAGAA+AGG 0.402455 5_3:-63699548 Msa0824440:CDS
GGCGGGAACGATGGCGGTGT+TGG 0.404837 5_3:+63700724 None:intergenic
CTACATCTCGATAAGACATC+TGG 0.408588 5_3:-63697664 Msa0824440:exon
TTTGTCTGGAGTTCATAGTT+TGG 0.413459 5_3:-63699336 Msa0824440:CDS
CACTTGCACTGCCCATGTCC+TGG 0.433645 5_3:-63697817 Msa0824440:CDS
AAAGGATCCTTTGCAGATGC+GGG 0.433799 5_3:-63699521 Msa0824440:CDS
ATGGCGGTGTTGGGAGAATT+GGG 0.443939 5_3:+63700734 None:intergenic
TGCATCTTGAAAAGACTTCG+AGG 0.462635 5_3:-63699308 Msa0824440:CDS
AAGTAGTTACTGAGAGAGTG+AGG 0.469748 5_3:-63700941 Msa0824440:five_prime_UTR
TGACGAAACATTGTTATCCT+AGG 0.471304 5_3:-63697534 Msa0824440:three_prime_UTR
AATATATGTAATGATTGATT+GGG 0.474749 5_3:-63697437 Msa0824440:three_prime_UTR
GCGAATAGTTTGAACTTGAA+AGG 0.486293 5_3:-63697460 Msa0824440:three_prime_UTR
GGAGGGAGCGGCGGGAACGA+TGG 0.491257 5_3:+63700715 None:intergenic
CAGATGTCTTATCGAGATGT+AGG 0.501456 5_3:+63697665 None:intergenic
TTTGGAAGAGGGAGTTGAAG+GGG 0.501908 5_3:+63697752 None:intergenic
TTGGAAGAGGGAGTTGAAGG+GGG 0.503925 5_3:+63697753 None:intergenic
CTTTCAATTCATTTGGAAGA+GGG 0.504674 5_3:+63697741 None:intergenic
AAGACAAACGGAGGGAGCGG+CGG 0.509174 5_3:+63700706 None:intergenic
AGACAAACGGAGGGAGCGGC+GGG 0.510021 5_3:+63700707 None:intergenic
TTCGCCACTGATTCCGCCGG+TGG 0.514250 5_3:-63700677 Msa0824440:CDS
ACTGTTACTCACTTCAGTCT+TGG 0.515228 5_3:+63700983 None:intergenic
CGGCGGAATCAGTGGCGAAA+CGG 0.519179 5_3:+63700681 None:intergenic
ATTTGGAAGAGGGAGTTGAA+GGG 0.523382 5_3:+63697751 None:intergenic
CAACCCTGCAAAAGCGCTTG+TGG 0.524162 5_3:+63697896 None:intergenic
AGAATTGGGAACGGAGGAGT+CGG 0.527066 5_3:+63700748 None:intergenic
TCTTTCAATTCATTTGGAAG+AGG 0.530636 5_3:+63697740 None:intergenic
GACATGGGCAGTGCAAGTGC+TGG 0.532373 5_3:+63697821 None:intergenic
ACTGAAGTGAGTAACAGTTA+TGG 0.534500 5_3:-63700978 Msa0824440:five_prime_UTR
GATAAGACAAACGGAGGGAG+CGG 0.540270 5_3:+63700703 None:intergenic
AGCGCCACCGGCGGAATCAG+TGG 0.542690 5_3:+63700673 None:intergenic
TAAAGGATCCTTTGCAGATG+CGG 0.548481 5_3:-63699522 Msa0824440:CDS
AGCAACAGTAGTGGCAGCAA+TGG 0.550223 5_3:-63700822 Msa0824440:exon
AGACTTCGAGGAAAGGATGA+TGG 0.553368 5_3:-63699296 Msa0824440:intron
ATGTCAACAACACATCTCCT+AGG 0.553581 5_3:+63697517 None:intergenic
GGAGTAGCTGGATGTTGCAC+GGG 0.556294 5_3:-63699178 Msa0824440:CDS
CTTGAAAAGACTTCGAGGAA+AGG 0.562655 5_3:-63699303 Msa0824440:CDS
CGTTTCGCCACTGATTCCGC+CGG 0.563253 5_3:-63700680 Msa0824440:CDS
GGGAGCGGCGGGAACGATGG+CGG 0.565959 5_3:+63700718 None:intergenic
CGAGAAAAGCAATGATATGT+TGG 0.566030 5_3:+63697483 None:intergenic
TGGAGTAGCTGGATGTTGCA+CGG 0.566090 5_3:-63699179 Msa0824440:CDS
TACATCTCGATAAGACATCT+GGG 0.575382 5_3:-63697663 Msa0824440:exon
AACCTAAATAAAAGCGAAGG+AGG 0.579757 5_3:+63697601 None:intergenic
TGCCCATGTCCTGGAAAACA+GGG 0.580299 5_3:-63697808 Msa0824440:CDS
AGAAGACCCCGCATCTGCAA+AGG 0.582837 5_3:+63699514 None:intergenic
ATATATGCAGTCATTAACGC+TGG 0.589905 5_3:-63699199 Msa0824440:intron
AAGAAAATGCCCCAAAAGTG+AGG 0.594359 5_3:+63697873 None:intergenic
GGAACCCATTAAAGCGCCAC+CGG 0.594398 5_3:+63700661 None:intergenic
ATGGCGACGGCGGATTCCGA+CGG 0.594896 5_3:-63700803 Msa0824440:CDS
AATACACTTATGTGAAGTAG+TGG 0.596345 5_3:-63697389 Msa0824440:three_prime_UTR
GGCGAAACGGATAAGACAAA+CGG 0.596943 5_3:+63700694 None:intergenic
GGGTGCATATCTTATCTACA+TGG 0.605368 5_3:+63697622 None:intergenic
ACCCATTAAAGCGCCACCGG+CGG 0.607759 5_3:+63700664 None:intergenic
ACCTAAATAAAAGCGAAGGA+GGG 0.609773 5_3:+63697602 None:intergenic
ATTGGGAACGGAGGAGTCGG+AGG 0.612660 5_3:+63700751 None:intergenic
AGTGGCAGCAATGGCGACGG+CGG 0.615157 5_3:-63700813 Msa0824440:exon
AGTAGTGGCAGCAATGGCGA+CGG 0.625713 5_3:-63700816 Msa0824440:exon
AACCAAAACAGCAACAGTAG+TGG 0.631961 5_3:-63700831 Msa0824440:five_prime_UTR
GAGGAATCGTCGTCATGAGG+CGG 0.635732 5_3:+63700770 None:intergenic
TCGGAGGAATCGTCGTCATG+AGG 0.636042 5_3:+63700767 None:intergenic
AGTAGTTACTGAGAGAGTGA+GGG 0.643075 5_3:-63700940 Msa0824440:five_prime_UTR
AGATGCGGGGTCTTCTGCAA+AGG 0.644010 5_3:-63699507 Msa0824440:intron
TTATGTGAAGTAGTGGCAGT+CGG 0.646364 5_3:-63697382 Msa0824440:three_prime_UTR
AAGGATCCTTTGCAGATGCG+GGG 0.647122 5_3:-63699520 Msa0824440:CDS
AGGAACCTAAATAAAAGCGA+AGG 0.647126 5_3:+63697598 None:intergenic
AGGCGGTGTTGTGATACCGT+CGG 0.666507 5_3:+63700787 None:intergenic
AATTGAAAGAGATAACGCAT+AGG 0.675806 5_3:-63700886 Msa0824440:five_prime_UTR
GGTGTTGGGAGAATTGGGAA+CGG 0.678466 5_3:+63700739 None:intergenic
GAAACGGATAAGACAAACGG+AGG 0.691757 5_3:+63700697 None:intergenic
AAACGGATAAGACAAACGGA+GGG 0.704958 5_3:+63700698 None:intergenic
GTTGGGAGAATTGGGAACGG+AGG 0.705073 5_3:+63700742 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAACTAAAACTGAAT+TGG + chr5_3:63697745-63697764 None:intergenic 15.0%
!!! TTTAAAAATTGTTGTTTGTT+TGG - chr5_3:63698187-63698206 Msa0824440:intron 15.0%
!!! AAACATTTTTGCTTTAATAA+CGG - chr5_3:63699063-63699082 Msa0824440:intron 15.0%
!! TTTAAAAGTTAAAAGAATAC+CGG + chr5_3:63699181-63699200 None:intergenic 15.0%
!!! TACTTCAATTTTGAAAAATT+GGG + chr5_3:63699529-63699548 None:intergenic 15.0%
!! AATATATGTAATGATTGATT+GGG - chr5_3:63700879-63700898 Msa0824440:five_prime_UTR 15.0%
!!! TAATTTTCATGTTTTAATCG+AGG - chr5_3:63697761-63697780 Msa0824440:CDS 20.0%
!!! AATTTTCATGTTTTAATCGA+GGG - chr5_3:63697762-63697781 Msa0824440:CDS 20.0%
!!! GCATTTTATCAAAATTTTGT+AGG + chr5_3:63698008-63698027 None:intergenic 20.0%
!! TTTGTGATTTCATTGAATAA+TGG + chr5_3:63698030-63698049 None:intergenic 20.0%
!!! ATAAACTATGTATGTGTTTT+TGG + chr5_3:63698372-63698391 None:intergenic 20.0%
!!! AACACATACATAGTTTATTT+TGG - chr5_3:63698374-63698393 Msa0824440:intron 20.0%
!! TATCATAGTTAGAAAATAAG+AGG + chr5_3:63698519-63698538 None:intergenic 20.0%
!! TGATATATGATAGAACATTA+TGG - chr5_3:63698534-63698553 Msa0824440:intron 20.0%
!!! TTTTCTGATTATTATGCTTA+AGG - chr5_3:63698719-63698738 Msa0824440:intron 20.0%
!!! TTTAAGAAGAAAGGTTTTAA+AGG - chr5_3:63698777-63698796 Msa0824440:intron 20.0%
!! TTAAAAGTTAAAAGAATACC+GGG + chr5_3:63699180-63699199 None:intergenic 20.0%
!!! CTACTTCAATTTTGAAAAAT+TGG + chr5_3:63699530-63699549 None:intergenic 20.0%
!! TCATAAATTATAAGAAACAG+AGG + chr5_3:63699831-63699850 None:intergenic 20.0%
!! ATTTATGAACTAATGAGTAA+TGG - chr5_3:63699843-63699862 Msa0824440:intron 20.0%
!! GAATATTAAACTATGTTCAT+AGG + chr5_3:63699889-63699908 None:intergenic 20.0%
!! AGTTTAATATTCTGATTTAG+AGG - chr5_3:63699897-63699916 Msa0824440:intron 20.0%
!!! TCTATCTACGATAATTTTTA+GGG - chr5_3:63699928-63699947 Msa0824440:intron 20.0%
!!! CATTTTCTTTTATTATGGAA+GGG - chr5_3:63700457-63700476 Msa0824440:intron 20.0%
!!! TTTTATCAGCATTTTGTTTT+TGG - chr5_3:63700747-63700766 Msa0824440:CDS 20.0%
!! GAATATATGTAATGATTGAT+TGG - chr5_3:63700878-63700897 Msa0824440:five_prime_UTR 20.0%
!! TTCTTAAATTATTCAGTGTT+TGG + chr5_3:63700964-63700983 None:intergenic 20.0%
!! AACTAAAAGAGGCATAATTA+AGG + chr5_3:63697846-63697865 None:intergenic 25.0%
!!! TAATTATGCCTCTTTTAGTT+TGG - chr5_3:63697846-63697865 Msa0824440:CDS 25.0%
! TTGAAAATCCAAACTAAAAG+AGG + chr5_3:63697857-63697876 None:intergenic 25.0%
!!! TCAATTTACCGTTTTAATCA+AGG - chr5_3:63697873-63697892 Msa0824440:CDS 25.0%
!!! CAATTTACCGTTTTAATCAA+GGG - chr5_3:63697874-63697893 Msa0824440:CDS 25.0%
! TTCAATGAAATCACAAATGT+TGG - chr5_3:63698033-63698052 Msa0824440:intron 25.0%
!! AAGTGAAACTGATTCTTATA+TGG - chr5_3:63698140-63698159 Msa0824440:intron 25.0%
!!! ATTGTTGTTTGTTTGGAATT+AGG - chr5_3:63698194-63698213 Msa0824440:intron 25.0%
!!! TTGTTGTTTGTTTGGAATTA+GGG - chr5_3:63698195-63698214 Msa0824440:intron 25.0%
! TATAACAAACATTGTCTGAA+AGG + chr5_3:63698342-63698361 None:intergenic 25.0%
!! GTTAACAAGAAATAGCATTT+TGG - chr5_3:63698486-63698505 Msa0824440:intron 25.0%
!! TGAAAGTATAGACATAATCA+GGG + chr5_3:63699098-63699117 None:intergenic 25.0%
!! ATGAAAGTATAGACATAATC+AGG + chr5_3:63699099-63699118 None:intergenic 25.0%
! TATAAAGAAAGAAGGGTAAA+GGG - chr5_3:63699251-63699270 Msa0824440:intron 25.0%
! GACTATATTACAGCTATTTA+AGG + chr5_3:63699340-63699359 None:intergenic 25.0%
!! TTTTCAAAATTGAAGTAGTC+CGG - chr5_3:63699532-63699551 Msa0824440:CDS 25.0%
!!! AATTTTTTATAAACCAGACC+CGG + chr5_3:63699554-63699573 None:intergenic 25.0%
!! GTCTGGTTTATAAAAAATTC+AGG - chr5_3:63699555-63699574 Msa0824440:intron 25.0%
!! CTCTATCTACGATAATTTTT+AGG - chr5_3:63699927-63699946 Msa0824440:intron 25.0%
! ATCAATGATTAGAACTTAGA+AGG + chr5_3:63700299-63700318 None:intergenic 25.0%
!!! GTTTCATTTTTGTACTTGTT+TGG - chr5_3:63700361-63700380 Msa0824440:intron 25.0%
!! GCATTTTCTTTTATTATGGA+AGG - chr5_3:63700456-63700475 Msa0824440:intron 25.0%
! AAAGCTTCTTTCAATTCATT+TGG + chr5_3:63700585-63700604 None:intergenic 25.0%
! TACTTCACATAAGTGTATTT+CGG + chr5_3:63700926-63700945 None:intergenic 25.0%
AATTGAAAGAGATAACGCAT+AGG - chr5_3:63697430-63697449 Msa0824440:three_prime_UTR 30.0%
AGAAAAACCGTATCCAAAAA+CGG + chr5_3:63697679-63697698 None:intergenic 30.0%
CACTAAACCCTTGATTAAAA+CGG + chr5_3:63697884-63697903 None:intergenic 30.0%
! ATACATTGATTGTCAATGCT+TGG - chr5_3:63697919-63697938 Msa0824440:intron 30.0%
ACTAACAACAATAAGCTTTG+TGG + chr5_3:63698113-63698132 None:intergenic 30.0%
!!! ACTATGTATGTGTTTTTGGT+TGG + chr5_3:63698368-63698387 None:intergenic 30.0%
!!! TTATTTTGGAAGTGACAAGA+TGG - chr5_3:63698388-63698407 Msa0824440:intron 30.0%
CATCTTAGTCAGAAAATAAG+AGG + chr5_3:63698640-63698659 None:intergenic 30.0%
! TTTTTGATACCTCAATTAGC+TGG - chr5_3:63698693-63698712 Msa0824440:intron 30.0%
AAACTGCAAATGTCTAACAA+AGG + chr5_3:63698962-63698981 None:intergenic 30.0%
! GAAAGTATAGACATAATCAG+GGG + chr5_3:63699097-63699116 None:intergenic 30.0%
TGGAACAAGTTTCAAAGAAA+GGG + chr5_3:63699220-63699239 None:intergenic 30.0%
TCTTTCTTTATACGCCAATA+AGG + chr5_3:63699243-63699262 None:intergenic 30.0%
TATTGGCGTATAAAGAAAGA+AGG - chr5_3:63699243-63699262 Msa0824440:intron 30.0%
ATTGGCGTATAAAGAAAGAA+GGG - chr5_3:63699244-63699263 Msa0824440:intron 30.0%
GTATAAAGAAAGAAGGGTAA+AGG - chr5_3:63699250-63699269 Msa0824440:intron 30.0%
TAATATAGTCCCTTATTGGT+GGG - chr5_3:63699350-63699369 Msa0824440:CDS 30.0%
TTTCAAAATTGAAGTAGTCC+GGG - chr5_3:63699533-63699552 Msa0824440:CDS 30.0%
! ACTTTTGGATTCCTTAACAA+GGG + chr5_3:63699969-63699988 None:intergenic 30.0%
! TACTTTTGGATTCCTTAACA+AGG + chr5_3:63699970-63699989 None:intergenic 30.0%
CTTATCATCTAGTATTCTTC+AGG - chr5_3:63700393-63700412 Msa0824440:intron 30.0%
!! TGGGGCATTTTCTTTTATTA+TGG - chr5_3:63700452-63700471 Msa0824440:intron 30.0%
CTTTCAATTCATTTGGAAGA+GGG + chr5_3:63700578-63700597 None:intergenic 30.0%
TCTTTCAATTCATTTGGAAG+AGG + chr5_3:63700579-63700598 None:intergenic 30.0%
!!! AGAAGCTTTCTCTTTTTTCT+CGG - chr5_3:63700596-63700615 Msa0824440:intron 30.0%
AAATGCTGATAAAAACTGCA+AGG + chr5_3:63700741-63700760 None:intergenic 30.0%
!! CAACATATCATTGCTTTTCT+CGG - chr5_3:63700834-63700853 Msa0824440:five_prime_UTR 30.0%
AATACACTTATGTGAAGTAG+TGG - chr5_3:63700927-63700946 Msa0824440:five_prime_UTR 30.0%
ACTGAAGTGAGTAACAGTTA+TGG - chr5_3:63697338-63697357 Msa0824440:three_prime_UTR 35.0%
ATTGTCAATGCTTGGAATAC+TGG - chr5_3:63697927-63697946 Msa0824440:intron 35.0%
TATGAAACACAGACACGAAA+CGG - chr5_3:63697971-63697990 Msa0824440:intron 35.0%
AAATGAACTGAAGTTGTGCT+TGG + chr5_3:63698275-63698294 None:intergenic 35.0%
CAACAACAACAACAACAACA+AGG + chr5_3:63698603-63698622 None:intergenic 35.0%
ATGCTTAAGGATGCTTGTAA+TGG - chr5_3:63698732-63698751 Msa0824440:intron 35.0%
TGCTTAAGGATGCTTGTAAT+GGG - chr5_3:63698733-63698752 Msa0824440:intron 35.0%
GCTGGATTGTTTAAGAAGAA+AGG - chr5_3:63698768-63698787 Msa0824440:intron 35.0%
!! TAGACATTTGCAGTTTTGTC+TGG - chr5_3:63698966-63698985 Msa0824440:intron 35.0%
! TTTGTCTGGAGTTCATAGTT+TGG - chr5_3:63698980-63698999 Msa0824440:intron 35.0%
ATATATGCAGTCATTAACGC+TGG - chr5_3:63699117-63699136 Msa0824440:intron 35.0%
GTGGAACAAGTTTCAAAGAA+AGG + chr5_3:63699221-63699240 None:intergenic 35.0%
! TGAAACTTGTTCCACCTTAT+TGG - chr5_3:63699226-63699245 Msa0824440:intron 35.0%
TTCTTTATACGCCAATAAGG+TGG + chr5_3:63699240-63699259 None:intergenic 35.0%
GCTGTAATATAGTCCCTTAT+TGG - chr5_3:63699346-63699365 Msa0824440:CDS 35.0%
GTAATATAGTCCCTTATTGG+TGG - chr5_3:63699349-63699368 Msa0824440:CDS 35.0%
!!! TTTTTAGTTGTATTCCACCG+TGG - chr5_3:63699382-63699401 Msa0824440:intron 35.0%
ACTTCTTGAATCCTAGAATC+AGG + chr5_3:63699456-63699475 None:intergenic 35.0%
GCATGAAGTAACAAAAACAG+AGG + chr5_3:63699748-63699767 None:intergenic 35.0%
TTCAACCTCTGTTCTGTAAA+TGG - chr5_3:63699787-63699806 Msa0824440:intron 35.0%
! CACTGCACAATTTCTACTTT+TGG + chr5_3:63699984-63700003 None:intergenic 35.0%
TTCCTTAACATGTACCCTTA+GGG - chr5_3:63700073-63700092 Msa0824440:intron 35.0%
AGTATACCATTTCGTACTAG+TGG - chr5_3:63700142-63700161 Msa0824440:intron 35.0%
GTATACCATTTCGTACTAGT+GGG - chr5_3:63700143-63700162 Msa0824440:intron 35.0%
GGTTAAAAGATCGTAACTCT+AGG + chr5_3:63700179-63700198 None:intergenic 35.0%
TTAAGAGTCTACGCAAGATT+GGG + chr5_3:63700206-63700225 None:intergenic 35.0%
ATTAAGAGTCTACGCAAGAT+TGG + chr5_3:63700207-63700226 None:intergenic 35.0%
!!! CTTTTTTTCCCTTTGCTTGA+CGG + chr5_3:63700249-63700268 None:intergenic 35.0%
TACATCTCGATAAGACATCT+GGG - chr5_3:63700653-63700672 Msa0824440:CDS 35.0%
ACCTAAATAAAAGCGAAGGA+GGG + chr5_3:63700717-63700736 None:intergenic 35.0%
AACCTAAATAAAAGCGAAGG+AGG + chr5_3:63700718-63700737 None:intergenic 35.0%
AGGAACCTAAATAAAAGCGA+AGG + chr5_3:63700721-63700740 None:intergenic 35.0%
TGACGAAACATTGTTATCCT+AGG - chr5_3:63700782-63700801 Msa0824440:CDS 35.0%
CGAGAAAAGCAATGATATGT+TGG + chr5_3:63700836-63700855 None:intergenic 35.0%
GCGAATAGTTTGAACTTGAA+AGG - chr5_3:63700856-63700875 Msa0824440:five_prime_UTR 35.0%
ACTGTTACTCACTTCAGTCT+TGG + chr5_3:63697336-63697355 None:intergenic 40.0%
AAGTAGTTACTGAGAGAGTG+AGG - chr5_3:63697375-63697394 Msa0824440:three_prime_UTR 40.0%
AGTAGTTACTGAGAGAGTGA+GGG - chr5_3:63697376-63697395 Msa0824440:three_prime_UTR 40.0%
!! TCGTTTTTGATGCGTTGTTG+TGG + chr5_3:63697464-63697483 None:intergenic 40.0%
AACCAAAACAGCAACAGTAG+TGG - chr5_3:63697485-63697504 Msa0824440:three_prime_UTR 40.0%
AAACGGATAAGACAAACGGA+GGG + chr5_3:63697621-63697640 None:intergenic 40.0%
!!! GCTTTAATGGGTTCCGTTTT+TGG - chr5_3:63697663-63697682 Msa0824440:exon 40.0%
!! ATGGGTTCCGTTTTTGGATA+CGG - chr5_3:63697669-63697688 Msa0824440:exon 40.0%
AAATGTTGGTGTGTCCATGT+CGG - chr5_3:63698047-63698066 Msa0824440:intron 40.0%
! CTTCTGATCAAAAGTGTGTC+CGG + chr5_3:63698076-63698095 None:intergenic 40.0%
AGAAAAACGCCAGCTAATTG+AGG + chr5_3:63698705-63698724 None:intergenic 40.0%
TAAAGGATCCTTTGCAGATG+CGG - chr5_3:63698794-63698813 Msa0824440:intron 40.0%
AAGTCGACATCAAGCAATCA+AGG + chr5_3:63698861-63698880 None:intergenic 40.0%
TGCATCTTGAAAAGACTTCG+AGG - chr5_3:63699008-63699027 Msa0824440:intron 40.0%
CTTGAAAAGACTTCGAGGAA+AGG - chr5_3:63699013-63699032 Msa0824440:intron 40.0%
!! AAAGGCACTTCAGCAAGTTA+AGG + chr5_3:63699479-63699498 None:intergenic 40.0%
!! TTAACTTGCTGAAGTGCCTT+TGG - chr5_3:63699478-63699497 Msa0824440:intron 40.0%
ATCTCACCATTGTGATCCAA+AGG + chr5_3:63699497-63699516 None:intergenic 40.0%
TTGCTAGTGGAAAAACGAAG+CGG + chr5_3:63699676-63699695 None:intergenic 40.0%
ACAGAGAAACGAGGAAAAGT+GGG + chr5_3:63699710-63699729 None:intergenic 40.0%
AACAGAGAAACGAGGAAAAG+TGG + chr5_3:63699711-63699730 None:intergenic 40.0%
ATGCAAGAAACAGAGAAACG+AGG + chr5_3:63699719-63699738 None:intergenic 40.0%
! GTTTCTCTGTTTCTTGCATG+TGG - chr5_3:63699720-63699739 Msa0824440:intron 40.0%
TGAAGCCATTTACAGAACAG+AGG + chr5_3:63699795-63699814 None:intergenic 40.0%
GAGTAATGGTTATGAACCTG+TGG - chr5_3:63699857-63699876 Msa0824440:intron 40.0%
TGGTTATGAACCTGTGGATT+TGG - chr5_3:63699863-63699882 Msa0824440:intron 40.0%
GTTCCTTAACATGTACCCTT+AGG - chr5_3:63700072-63700091 Msa0824440:intron 40.0%
AATGCTAATGTGTGCCCTAA+GGG + chr5_3:63700090-63700109 None:intergenic 40.0%
AAATGCTAATGTGTGCCCTA+AGG + chr5_3:63700091-63700110 None:intergenic 40.0%
AAGATCCCACTAGTACGAAA+TGG + chr5_3:63700151-63700170 None:intergenic 40.0%
CGTCAAGCAAAGGGAAAAAA+AGG - chr5_3:63700247-63700266 Msa0824440:intron 40.0%
AAGAAAATGCCCCAAAAGTG+AGG + chr5_3:63700446-63700465 None:intergenic 40.0%
ATTTGGAAGAGGGAGTTGAA+GGG + chr5_3:63700568-63700587 None:intergenic 40.0%
! TCTTTGAAAAAGCGCAGCAA+GGG - chr5_3:63700621-63700640 Msa0824440:intron 40.0%
CAGATGTCTTATCGAGATGT+AGG + chr5_3:63700654-63700673 None:intergenic 40.0%
CTACATCTCGATAAGACATC+TGG - chr5_3:63700652-63700671 Msa0824440:CDS 40.0%
GGGTGCATATCTTATCTACA+TGG + chr5_3:63700697-63700716 None:intergenic 40.0%
!! ACCCTCCTTCGCTTTTATTT+AGG - chr5_3:63700713-63700732 Msa0824440:CDS 40.0%
ATGTCAACAACACATCTCCT+AGG + chr5_3:63700802-63700821 None:intergenic 40.0%
TTATGTGAAGTAGTGGCAGT+CGG - chr5_3:63700934-63700953 Msa0824440:five_prime_UTR 40.0%
!!! TGCCACTACTGTTGCTGTTT+TGG + chr5_3:63697490-63697509 None:intergenic 45.0%
GAAACGGATAAGACAAACGG+AGG + chr5_3:63697622-63697641 None:intergenic 45.0%
GGCGAAACGGATAAGACAAA+CGG + chr5_3:63697625-63697644 None:intergenic 45.0%
! AAGGCCTTATCCCACTTAGT+GGG + chr5_3:63698584-63698603 None:intergenic 45.0%
!! GCTTGTAATGGGTGTGTTTC+AGG - chr5_3:63698744-63698763 Msa0824440:intron 45.0%
AAAGGATCCTTTGCAGATGC+GGG - chr5_3:63698795-63698814 Msa0824440:intron 45.0%
AGACTTCGAGGAAAGGATGA+TGG - chr5_3:63699020-63699039 Msa0824440:intron 45.0%
!! GGACTTGCTCTAAGTTTTCC+CGG - chr5_3:63699159-63699178 Msa0824440:CDS 45.0%
ATAACCTCGCCCACCAATAA+GGG + chr5_3:63699362-63699381 None:intergenic 45.0%
AATAACCTCGCCCACCAATA+AGG + chr5_3:63699363-63699382 None:intergenic 45.0%
! GAAGTGCCTTTGGATCACAA+TGG - chr5_3:63699488-63699507 Msa0824440:intron 45.0%
AAATTGAAGTAGTCCGGGTC+TGG - chr5_3:63699538-63699557 Msa0824440:CDS 45.0%
TGTTCATAGGCCAAATCCAC+AGG + chr5_3:63699876-63699895 None:intergenic 45.0%
! TCCTTAACAAGGGCACACAT+TGG + chr5_3:63699959-63699978 None:intergenic 45.0%
! TGCCCTAAGGGTACATGTTA+AGG + chr5_3:63700078-63700097 None:intergenic 45.0%
TTGTCATCACCGTCAAGCAA+AGG - chr5_3:63700237-63700256 Msa0824440:intron 45.0%
TGTCATCACCGTCAAGCAAA+GGG - chr5_3:63700238-63700257 Msa0824440:intron 45.0%
TTTGGAAGAGGGAGTTGAAG+GGG + chr5_3:63700567-63700586 None:intergenic 45.0%
CATTTGGAAGAGGGAGTTGA+AGG + chr5_3:63700569-63700588 None:intergenic 45.0%
! GTCTTTGAAAAAGCGCAGCA+AGG - chr5_3:63700620-63700639 Msa0824440:intron 45.0%
AGCAACAGTAGTGGCAGCAA+TGG - chr5_3:63697494-63697513 Msa0824440:three_prime_UTR 50.0%
AGAATTGGGAACGGAGGAGT+CGG + chr5_3:63697571-63697590 None:intergenic 50.0%
! GGTGTTGGGAGAATTGGGAA+CGG + chr5_3:63697580-63697599 None:intergenic 50.0%
! ATGGCGGTGTTGGGAGAATT+GGG + chr5_3:63697585-63697604 None:intergenic 50.0%
GATAAGACAAACGGAGGGAG+CGG + chr5_3:63697616-63697635 None:intergenic 50.0%
ACTTAGTGGGGTAGGCTACA+TGG + chr5_3:63698571-63698590 None:intergenic 50.0%
ATGTAGCCTACCCCACTAAG+TGG - chr5_3:63698570-63698589 Msa0824440:intron 50.0%
TGTAGCCTACCCCACTAAGT+GGG - chr5_3:63698571-63698590 Msa0824440:intron 50.0%
CTTATCCCACTTAGTGGGGT+AGG + chr5_3:63698579-63698598 None:intergenic 50.0%
CTACCCCACTAAGTGGGATA+AGG - chr5_3:63698577-63698596 Msa0824440:intron 50.0%
! AGGCCTTATCCCACTTAGTG+GGG + chr5_3:63698583-63698602 None:intergenic 50.0%
! CAAGGCCTTATCCCACTTAG+TGG + chr5_3:63698585-63698604 None:intergenic 50.0%
! AATGGGTGTGTTTCAGGTGC+TGG - chr5_3:63698750-63698769 Msa0824440:intron 50.0%
AAGGATCCTTTGCAGATGCG+GGG - chr5_3:63698796-63698815 Msa0824440:intron 50.0%
GTCATTAACGCTGGAGTAGC+TGG - chr5_3:63699126-63699145 Msa0824440:intron 50.0%
TGGAGTAGCTGGATGTTGCA+CGG - chr5_3:63699137-63699156 Msa0824440:intron 50.0%
!! ACACTGGATGGTGGATTCCA+CGG + chr5_3:63699402-63699421 None:intergenic 50.0%
TGGAATCCACCATCCAGTGT+TGG - chr5_3:63699402-63699421 Msa0824440:intron 50.0%
! AATCCACCATCCAGTGTTGG+TGG - chr5_3:63699405-63699424 Msa0824440:intron 50.0%
ATTCCACCAACACTGGATGG+TGG + chr5_3:63699411-63699430 None:intergenic 50.0%
GAGATTCCACCAACACTGGA+TGG + chr5_3:63699414-63699433 None:intergenic 50.0%
ACTGGAGATTCCACCAACAC+TGG + chr5_3:63699418-63699437 None:intergenic 50.0%
GGAATCTCCAGTTGAAGCCT+AGG - chr5_3:63699426-63699445 Msa0824440:intron 50.0%
TCCTAGAATCAGGCGATCCT+AGG + chr5_3:63699446-63699465 None:intergenic 50.0%
GGAGGAGGTAGTATTGCTAG+TGG + chr5_3:63699689-63699708 None:intergenic 50.0%
AAACGAGGAAAAGTGGGAGG+AGG + chr5_3:63699704-63699723 None:intergenic 50.0%
GAGAAACGAGGAAAAGTGGG+AGG + chr5_3:63699707-63699726 None:intergenic 50.0%
GCCAATGTGTGCCCTTGTTA+AGG - chr5_3:63699955-63699974 Msa0824440:intron 50.0%
AGTCTACGCAAGATTGGGAG+GGG + chr5_3:63700201-63700220 None:intergenic 50.0%
GAGTCTACGCAAGATTGGGA+GGG + chr5_3:63700202-63700221 None:intergenic 50.0%
AGAGTCTACGCAAGATTGGG+AGG + chr5_3:63700203-63700222 None:intergenic 50.0%
! TTGCAGGGTTGCCTCACTTT+TGG - chr5_3:63700432-63700451 Msa0824440:intron 50.0%
!! TGCAGGGTTGCCTCACTTTT+GGG - chr5_3:63700433-63700452 Msa0824440:intron 50.0%
TGCCCATGTCCTGGAAAACA+GGG - chr5_3:63700508-63700527 Msa0824440:intron 50.0%
! AGCCCTGTTTTCCAGGACAT+GGG + chr5_3:63700513-63700532 None:intergenic 50.0%
! AGTTGAAGGGGGAGTACAAG+GGG + chr5_3:63700555-63700574 None:intergenic 50.0%
! GAGTTGAAGGGGGAGTACAA+GGG + chr5_3:63700556-63700575 None:intergenic 50.0%
TTGGAAGAGGGAGTTGAAGG+GGG + chr5_3:63700566-63700585 None:intergenic 50.0%
AGTAGTGGCAGCAATGGCGA+CGG - chr5_3:63697500-63697519 Msa0824440:three_prime_UTR 55.0%
!! AGGCGGTGTTGTGATACCGT+CGG + chr5_3:63697532-63697551 None:intergenic 55.0%
GAGGAATCGTCGTCATGAGG+CGG + chr5_3:63697549-63697568 None:intergenic 55.0%
TCGGAGGAATCGTCGTCATG+AGG + chr5_3:63697552-63697571 None:intergenic 55.0%
GTTGGGAGAATTGGGAACGG+AGG + chr5_3:63697577-63697596 None:intergenic 55.0%
! GATGGCGGTGTTGGGAGAAT+TGG + chr5_3:63697586-63697605 None:intergenic 55.0%
! GGAACCCATTAAAGCGCCAC+CGG + chr5_3:63697658-63697677 None:intergenic 55.0%
AGAAGACCCCGCATCTGCAA+AGG + chr5_3:63698805-63698824 None:intergenic 55.0%
AGATGCGGGGTCTTCTGCAA+AGG - chr5_3:63698809-63698828 Msa0824440:intron 55.0%
GGAGTAGCTGGATGTTGCAC+GGG - chr5_3:63699138-63699157 Msa0824440:intron 55.0%
! TAGTCCCTTATTGGTGGGCG+AGG - chr5_3:63699355-63699374 Msa0824440:intron 55.0%
AGGCGATCCTAGGCTTCAAC+TGG + chr5_3:63699436-63699455 None:intergenic 55.0%
GCCTAGGATCGCCTGATTCT+AGG - chr5_3:63699442-63699461 Msa0824440:intron 55.0%
GTCTACGCAAGATTGGGAGG+GGG + chr5_3:63700200-63700219 None:intergenic 55.0%
! TGCACCACAAGCGCTTTTGC+AGG - chr5_3:63700416-63700435 Msa0824440:intron 55.0%
! GCACCACAAGCGCTTTTGCA+GGG - chr5_3:63700417-63700436 Msa0824440:intron 55.0%
!! CAACCCTGCAAAAGCGCTTG+TGG + chr5_3:63700423-63700442 None:intergenic 55.0%
!! GCAGGGTTGCCTCACTTTTG+GGG - chr5_3:63700434-63700453 Msa0824440:intron 55.0%
CTGCCCATGTCCTGGAAAAC+AGG - chr5_3:63700507-63700526 Msa0824440:intron 55.0%
! CAGCCCTGTTTTCCAGGACA+TGG + chr5_3:63700514-63700533 None:intergenic 55.0%
! GGAGTTGAAGGGGGAGTACA+AGG + chr5_3:63700557-63700576 None:intergenic 55.0%
ATTGGGAACGGAGGAGTCGG+AGG + chr5_3:63697568-63697587 None:intergenic 60.0%
AAGACAAACGGAGGGAGCGG+CGG + chr5_3:63697613-63697632 None:intergenic 60.0%
CGGCGGAATCAGTGGCGAAA+CGG + chr5_3:63697638-63697657 None:intergenic 60.0%
!! CGTTTCGCCACTGATTCCGC+CGG - chr5_3:63697636-63697655 Msa0824440:three_prime_UTR 60.0%
TTCCGCCGGTGGCGCTTTAA+TGG - chr5_3:63697650-63697669 Msa0824440:three_prime_UTR 60.0%
TCCGCCGGTGGCGCTTTAAT+GGG - chr5_3:63697651-63697670 Msa0824440:three_prime_UTR 60.0%
! ACCCATTAAAGCGCCACCGG+CGG + chr5_3:63697655-63697674 None:intergenic 60.0%
GGTGTGTCCATGTCGGACAC+CGG - chr5_3:63698054-63698073 Msa0824440:intron 60.0%
AGTGTGTCCGGTGTCCGACA+TGG + chr5_3:63698064-63698083 None:intergenic 60.0%
!! CTGGATGGTGGATTCCACGG+TGG + chr5_3:63699399-63699418 None:intergenic 60.0%
ATCGCTGTCGCACAGGGAAG+AGG + chr5_3:63699623-63699642 None:intergenic 60.0%
GGTAGCATCGCTGTCGCACA+GGG + chr5_3:63699629-63699648 None:intergenic 60.0%
AGGTAGCATCGCTGTCGCAC+AGG + chr5_3:63699630-63699649 None:intergenic 60.0%
TGCTACCTCTCCTCCACCAG+CGG - chr5_3:63699642-63699661 Msa0824440:intron 60.0%
GACATGGGCAGTGCAAGTGC+TGG + chr5_3:63700498-63700517 None:intergenic 60.0%
CACTTGCACTGCCCATGTCC+TGG - chr5_3:63700499-63700518 Msa0824440:intron 60.0%
! GTGCTGCAGCCCTGTTTTCC+AGG + chr5_3:63700520-63700539 None:intergenic 60.0%
! TGAAGGGGGAGTACAAGGGG+AGG + chr5_3:63700552-63700571 None:intergenic 60.0%
AGTGGCAGCAATGGCGACGG+CGG - chr5_3:63697503-63697522 Msa0824440:three_prime_UTR 65.0%
! ATGGCGACGGCGGATTCCGA+CGG - chr5_3:63697513-63697532 Msa0824440:three_prime_UTR 65.0%
! GCGGGAACGATGGCGGTGTT+GGG + chr5_3:63697594-63697613 None:intergenic 65.0%
AGACAAACGGAGGGAGCGGC+GGG + chr5_3:63697612-63697631 None:intergenic 65.0%
!! TTCGCCACTGATTCCGCCGG+TGG - chr5_3:63697639-63697658 Msa0824440:three_prime_UTR 65.0%
! GGCGGGAACGATGGCGGTGT+TGG + chr5_3:63697595-63697614 None:intergenic 70.0%
AGCGCCACCGGCGGAATCAG+TGG + chr5_3:63697646-63697665 None:intergenic 70.0%
GGGAGCGGCGGGAACGATGG+CGG + chr5_3:63697601-63697620 None:intergenic 75.0%
GGAGGGAGCGGCGGGAACGA+TGG + chr5_3:63697604-63697623 None:intergenic 75.0%
CGCAGCCGCTGGTGGAGGAG+AGG + chr5_3:63699650-63699669 None:intergenic 75.0%
! GGTGTCGCAGCCGCTGGTGG+AGG + chr5_3:63699655-63699674 None:intergenic 75.0%
!! AGCGGTGTCGCAGCCGCTGG+TGG + chr5_3:63699658-63699677 None:intergenic 75.0%
!! CGAAGCGGTGTCGCAGCCGC+TGG + chr5_3:63699661-63699680 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 63697333 63701005 63697333 ID=Msa0824440;Name=Msa0824440
chr5_3 mRNA 63697333 63701005 63697333 ID=Msa0824440-mRNA-1;Parent=Msa0824440;Name=Msa0824440-mRNA-1;_AED=0.09;_eAED=0.09;_QI=180|1|1|1|1|1|5|340|206
chr5_3 exon 63697333 63697923 63697333 ID=Msa0824440-mRNA-1:exon:19895;Parent=Msa0824440-mRNA-1
chr5_3 exon 63699158 63699211 63699158 ID=Msa0824440-mRNA-1:exon:19894;Parent=Msa0824440-mRNA-1
chr5_3 exon 63699297 63699369 63699297 ID=Msa0824440-mRNA-1:exon:19893;Parent=Msa0824440-mRNA-1
chr5_3 exon 63699508 63699572 63699508 ID=Msa0824440-mRNA-1:exon:19892;Parent=Msa0824440-mRNA-1
chr5_3 exon 63700648 63701005 63700648 ID=Msa0824440-mRNA-1:exon:19891;Parent=Msa0824440-mRNA-1
chr5_3 five_prime_UTR 63700826 63701005 63700826 ID=Msa0824440-mRNA-1:five_prime_utr;Parent=Msa0824440-mRNA-1
chr5_3 CDS 63700648 63700825 63700648 ID=Msa0824440-mRNA-1:cds;Parent=Msa0824440-mRNA-1
chr5_3 CDS 63699508 63699572 63699508 ID=Msa0824440-mRNA-1:cds;Parent=Msa0824440-mRNA-1
chr5_3 CDS 63699297 63699369 63699297 ID=Msa0824440-mRNA-1:cds;Parent=Msa0824440-mRNA-1
chr5_3 CDS 63699158 63699211 63699158 ID=Msa0824440-mRNA-1:cds;Parent=Msa0824440-mRNA-1
chr5_3 CDS 63697673 63697923 63697673 ID=Msa0824440-mRNA-1:cds;Parent=Msa0824440-mRNA-1
chr5_3 three_prime_UTR 63697333 63697672 63697333 ID=Msa0824440-mRNA-1:three_prime_utr;Parent=Msa0824440-mRNA-1
Gene Sequence

>Msa0824440

ATGGCGACGGCGGATTCCGACGGTATCACAACACCGCCTCATGACGACGATTCCTCCGACTCCTCCGTTCCCAATTCTCCCAACACCGCCATCGTTCCCGCCGCTCCCTCCGTTTGTCTTATCCGTTTCGCCACTGATTCCGCCGGTGGCGCTTTAATGGGTTCCGTTTTTGGATACGGTGCTGGATTGTTTAAGAAGAAAGGTTTTAAAGGATCCTTTGCAGATGCGGGGTCTTCTGCAAAGACATTTGCAGTTTTGTCTGGAGTTCATAGTTTGGTTGTTTGCATCTTGAAAAGACTTCGAGGAAAGGATGATGTCATTAACGCTGGAGTAGCTGGATGTTGCACGGGACTTGCTCTAAGTTTTCCCGGTGCACCACAAGCGCTTTTGCAGGGTTGCCTCACTTTTGGGGCATTTTCTTTTATTATGGAAGGGCTAAACAAACAGCAGCCAGCACTTGCACTGCCCATGTCCTGGAAAACAGGGCTGCAGCACAATGCACGACCTCCCCTTGTACTCCCCCTTCAACTCCCTCTTCCAAATGAATTGAAAGAAGCTTTCTCTTTTTTCTCGGAGTCTTTGAAAAAGCGCAGCAAGGGAGCTTATCCTACATCTCGATAA
Protein sequence

>Msa0824440

MATADSDGITTPPHDDDSSDSSVPNSPNTAIVPAAPSVCLIRFATDSAGGALMGSVFGYGAGLFKKKGFKGSFADAGSSAKTFAVLSGVHSLVVCILKRLRGKDDVINAGVAGCCTGLALSFPGAPQALLQGCLTFGAFSFIMEGLNKQQPALALPMSWKTGLQHNARPPLVLPLQLPLPNELKEAFSFFSESLKKRSKGAYPTSR*