AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0787580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787580 MtrunA17_Chr5g0445201 94.118 204 12 0 1 204 1 204 6.27e-130 363
Msa0787580 MtrunA17_Chr2g0312891 49.254 67 34 0 77 143 96 162 6.69e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787580 AT4G26670.1 64.593 209 63 4 1 198 1 209 1.14e-82 244
Msa0787580 AT5G55510.1 63.542 192 70 0 14 205 23 214 2.23e-82 244
Msa0787580 AT3G10110.1 43.284 67 38 0 77 143 100 166 8.79e-12 61.6

Find 84 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCAGGGTTGCCTCACTTT+TGG 0.109323 5_2:-73491723 Msa0787580:CDS
CTCTACTACTGTTGCTGTTT+TGG 0.185304 5_2:+73494655 None:intergenic
TTCCGCCGGTGGCGCTTTAA+TGG 0.189872 5_2:-73494496 Msa0787580:CDS
CTGCCCATGTCCTGGAAAAC+AGG 0.282308 5_2:-73491648 Msa0787580:CDS
AAAGCTTCTTTCAATTCATT+TGG 0.319112 5_2:+73491573 None:intergenic
TTCTTAAATTATTCAGTGTT+TGG 0.319617 5_2:+73491194 None:intergenic
GATGGCGGTGTTGGGAGAAT+TGG 0.325669 5_2:+73494563 None:intergenic
TCCGCCGGTGGCGCTTTAAT+GGG 0.333280 5_2:-73494495 Msa0787580:CDS
TACTTCACATAAGTGTATTT+CGG 0.339634 5_2:+73491232 None:intergenic
GAATATATGTAATGATTGAT+TGG 0.359410 5_2:-73491277 Msa0787580:three_prime_UTR
GTCATTAACGCTGGAGTAGC+TGG 0.388658 5_2:-73493029 Msa0787580:CDS
GCGGGAACGATGGCGGTGTT+GGG 0.392409 5_2:+73494555 None:intergenic
CATTTGGAAGAGGGAGTTGA+AGG 0.400267 5_2:+73491589 None:intergenic
GCTGGATTGTTTAAGAAGAA+AGG 0.402455 5_2:-73493387 Msa0787580:CDS
GGCGGGAACGATGGCGGTGT+TGG 0.404837 5_2:+73494554 None:intergenic
CTACATCTCGATAAGACATC+TGG 0.408588 5_2:-73491503 Msa0787580:exon
TTTGTCTGGAGTTCATAGTT+TGG 0.413459 5_2:-73493175 Msa0787580:CDS
CACTTGCACTGCCCATGTCC+TGG 0.433645 5_2:-73491656 Msa0787580:CDS
AAAGGATCCTTTGCAGATGC+GGG 0.433799 5_2:-73493360 Msa0787580:CDS
TGCACCACAAGCGCTTCTGC+AGG 0.437995 5_2:-73491739 Msa0787580:CDS
ATGGCGGTGTTGGGAGAATT+GGG 0.443939 5_2:+73494564 None:intergenic
GTTAATTGTACTAATTGAAT+CGG 0.447561 5_2:-73491165 Msa0787580:three_prime_UTR
TGCATCTTGAAAAGACTTCG+AGG 0.462635 5_2:-73493147 Msa0787580:CDS
TGACGAAACATTGTTATCCT+AGG 0.471304 5_2:-73491373 Msa0787580:three_prime_UTR
AATATATGTAATGATTGATT+GGG 0.474749 5_2:-73491276 Msa0787580:three_prime_UTR
GCGAATAGTTTGAACTTGAA+AGG 0.486293 5_2:-73491299 Msa0787580:three_prime_UTR
GGAGGGAGCGGCGGGAACGA+TGG 0.491257 5_2:+73494545 None:intergenic
CAGATGTCTTATCGAGATGT+AGG 0.501456 5_2:+73491504 None:intergenic
TTTGGAAGAGGGAGTTGAAG+GGG 0.501908 5_2:+73491591 None:intergenic
TTGGAAGAGGGAGTTGAAGG+GGG 0.503925 5_2:+73491592 None:intergenic
CTTTCAATTCATTTGGAAGA+GGG 0.504674 5_2:+73491580 None:intergenic
AAGACAAACGGAGGGAGCGG+CGG 0.509174 5_2:+73494536 None:intergenic
AGACAAACGGAGGGAGCGGC+GGG 0.510021 5_2:+73494537 None:intergenic
TTCGCCACTGATTCCGCCGG+TGG 0.514250 5_2:-73494507 Msa0787580:CDS
ACTGTTACTCACTTCAGTCT+TGG 0.515228 5_2:+73494808 None:intergenic
CGGCGGAATCAGTGGCGAAA+CGG 0.519179 5_2:+73494511 None:intergenic
ATTTGGAAGAGGGAGTTGAA+GGG 0.523382 5_2:+73491590 None:intergenic
CAACCCTGCAGAAGCGCTTG+TGG 0.524375 5_2:+73491735 None:intergenic
AGAATTGGGAACGGAGGAGT+CGG 0.527066 5_2:+73494578 None:intergenic
TCTTTCAATTCATTTGGAAG+AGG 0.530636 5_2:+73491579 None:intergenic
GACATGGGCAGTGCAAGTGC+TGG 0.532373 5_2:+73491660 None:intergenic
ACTGAAGTGAGTAACAGTTA+TGG 0.534500 5_2:-73494803 Msa0787580:five_prime_UTR
GATAAGACAAACGGAGGGAG+CGG 0.540270 5_2:+73494533 None:intergenic
AGCGCCACCGGCGGAATCAG+TGG 0.542690 5_2:+73494503 None:intergenic
TAAAGGATCCTTTGCAGATG+CGG 0.548481 5_2:-73493361 Msa0787580:CDS
AGACTTCGAGGAAAGGATGA+TGG 0.553368 5_2:-73493135 Msa0787580:intron
ATGTCAACAACACATCTCCT+AGG 0.553581 5_2:+73491356 None:intergenic
GGAGTAGCTGGATGTTGCAC+GGG 0.556294 5_2:-73493017 Msa0787580:CDS
CTTGAAAAGACTTCGAGGAA+AGG 0.562655 5_2:-73493142 Msa0787580:CDS
CGTTTCGCCACTGATTCCGC+CGG 0.563253 5_2:-73494510 Msa0787580:CDS
GGGAGCGGCGGGAACGATGG+CGG 0.565959 5_2:+73494548 None:intergenic
CGAGAAAAGCAATGATATGT+TGG 0.566030 5_2:+73491322 None:intergenic
TGGAGTAGCTGGATGTTGCA+CGG 0.566090 5_2:-73493018 Msa0787580:CDS
TACATCTCGATAAGACATCT+GGG 0.575382 5_2:-73491502 Msa0787580:exon
AACCTAAATAAAAGCGAAGG+AGG 0.579757 5_2:+73491440 None:intergenic
TGCCCATGTCCTGGAAAACA+GGG 0.580299 5_2:-73491647 Msa0787580:CDS
AGAAGACCCCGCATCTGCAA+AGG 0.582837 5_2:+73493353 None:intergenic
GCACCACAAGCGCTTCTGCA+GGG 0.585746 5_2:-73491738 Msa0787580:CDS
ATATATGCAGTCATTAACGC+TGG 0.589905 5_2:-73493038 Msa0787580:intron
AAGAAAATGCCCCAAAAGTG+AGG 0.594359 5_2:+73491712 None:intergenic
GGAACCCATTAAAGCGCCAC+CGG 0.594398 5_2:+73494491 None:intergenic
ATGGCGACGGCGGATTCCGA+CGG 0.594896 5_2:-73494630 Msa0787580:CDS
AATACACTTATGTGAAGTAG+TGG 0.596345 5_2:-73491228 Msa0787580:three_prime_UTR
GGCGAAACGGATAAGACAAA+CGG 0.596943 5_2:+73494524 None:intergenic
GAAAGAGATAACGCATAGGT+AGG 0.598200 5_2:-73494709 Msa0787580:five_prime_UTR
GGGTGCATATCTTATCTACA+TGG 0.605368 5_2:+73491461 None:intergenic
ACCCATTAAAGCGCCACCGG+CGG 0.607759 5_2:+73494494 None:intergenic
ACCTAAATAAAAGCGAAGGA+GGG 0.609773 5_2:+73491441 None:intergenic
ATTGGGAACGGAGGAGTCGG+AGG 0.612660 5_2:+73494581 None:intergenic
CAGCAACAGTAGTAGAGCAA+TGG 0.616319 5_2:-73494649 Msa0787580:exon
CAGTAGTAGAGCAATGGCGA+CGG 0.618507 5_2:-73494643 Msa0787580:exon
TAGTAGAGCAATGGCGACGG+CGG 0.629072 5_2:-73494640 Msa0787580:exon
TCGGAGGAATCTTCGTCATG+AGG 0.640872 5_2:+73494597 None:intergenic
AGATGCGGGGTCTTCTGCAA+AGG 0.644010 5_2:-73493346 Msa0787580:intron
TTATGTGAAGTAGTGGCAGT+CGG 0.646364 5_2:-73491221 Msa0787580:three_prime_UTR
AAGGATCCTTTGCAGATGCG+GGG 0.647122 5_2:-73493359 Msa0787580:CDS
AGGAACCTAAATAAAAGCGA+AGG 0.647126 5_2:+73491437 None:intergenic
ATGAGGCGGTGTGATACCGT+CGG 0.648572 5_2:+73494614 None:intergenic
GAGGAATCTTCGTCATGAGG+CGG 0.670657 5_2:+73494600 None:intergenic
AATTGAAAGAGATAACGCAT+AGG 0.675806 5_2:-73494713 Msa0787580:five_prime_UTR
GGTGTTGGGAGAATTGGGAA+CGG 0.678466 5_2:+73494569 None:intergenic
GAAACGGATAAGACAAACGG+AGG 0.691757 5_2:+73494527 None:intergenic
AAACGGATAAGACAAACGGA+GGG 0.704958 5_2:+73494528 None:intergenic
GTTGGGAGAATTGGGAACGG+AGG 0.705073 5_2:+73494572 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAACTAAAACTGAAT+TGG + chr5_2:73491572-73491591 None:intergenic 15.0%
!!! TTTAAATATTGTTGTTTGTT+TGG - chr5_2:73492014-73492033 Msa0787580:intron 15.0%
!!! AAACATTTTTGCTTTAATAA+CGG - chr5_2:73492881-73492900 Msa0787580:intron 15.0%
!! TTTAAAAGTTAAAAGAATAC+CGG + chr5_2:73492999-73493018 None:intergenic 15.0%
!!! TACTTCAATTTTGAAAAATT+GGG + chr5_2:73493347-73493366 None:intergenic 15.0%
!! AATATATGTAATGATTGATT+GGG - chr5_2:73494697-73494716 Msa0787580:five_prime_UTR 15.0%
!!! TAATTTTCATGTTTTAATCG+AGG - chr5_2:73491588-73491607 Msa0787580:CDS 20.0%
!!! AATTTTCATGTTTTAATCGA+GGG - chr5_2:73491589-73491608 Msa0787580:CDS 20.0%
!!! GCATTTTATCAAAATTTTGT+AGG + chr5_2:73491835-73491854 None:intergenic 20.0%
!! TTTGTGATTTCATTGAATAA+TGG + chr5_2:73491857-73491876 None:intergenic 20.0%
!! TATAACAAATATTGTCTGAA+AGG + chr5_2:73492169-73492188 None:intergenic 20.0%
!!! ATAAACTATGTATGTGTTTT+TGG + chr5_2:73492199-73492218 None:intergenic 20.0%
!!! AACACATACATAGTTTATTT+TGG - chr5_2:73492201-73492220 Msa0787580:intron 20.0%
!! TATCATAGTTAGAAAATAAG+AGG + chr5_2:73492346-73492365 None:intergenic 20.0%
!! TGATATATGATAGAACATTA+TGG - chr5_2:73492361-73492380 Msa0787580:intron 20.0%
!!! TTTTCTGATTATTATGCTTA+AGG - chr5_2:73492537-73492556 Msa0787580:intron 20.0%
!!! TTTAAGAAGAAAGGTTTTAA+AGG - chr5_2:73492595-73492614 Msa0787580:intron 20.0%
!! TTAAAAGTTAAAAGAATACC+GGG + chr5_2:73492998-73493017 None:intergenic 20.0%
!!! CTACTTCAATTTTGAAAAAT+TGG + chr5_2:73493348-73493367 None:intergenic 20.0%
!! TCATAAATTATAAGAAACAG+AGG + chr5_2:73493649-73493668 None:intergenic 20.0%
!! ATTTATGAACTAATGAGTAA+TGG - chr5_2:73493661-73493680 Msa0787580:intron 20.0%
!! GAATATTAAACTATGTTCAT+AGG + chr5_2:73493707-73493726 None:intergenic 20.0%
!! AGTTTAATATTCTGATTTAG+AGG - chr5_2:73493715-73493734 Msa0787580:intron 20.0%
!!! TCTATCTACGATAATTTTTA+GGG - chr5_2:73493746-73493765 Msa0787580:intron 20.0%
!!! CATTTTCTTTTATTATGGAA+GGG - chr5_2:73494275-73494294 Msa0787580:intron 20.0%
!!! TTTTATCAGCATTTTGTTTT+TGG - chr5_2:73494565-73494584 Msa0787580:CDS 20.0%
!! GAATATATGTAATGATTGAT+TGG - chr5_2:73494696-73494715 Msa0787580:five_prime_UTR 20.0%
!! TTCTTAAATTATTCAGTGTT+TGG + chr5_2:73494782-73494801 None:intergenic 20.0%
!! AACTAAAAGAGGCATAATTA+AGG + chr5_2:73491673-73491692 None:intergenic 25.0%
!!! TAATTATGCCTCTTTTAGTT+TGG - chr5_2:73491673-73491692 Msa0787580:CDS 25.0%
! TTGAAAATCCAAACTAAAAG+AGG + chr5_2:73491684-73491703 None:intergenic 25.0%
!!! TCAATTTACCGTTTTAATCA+AGG - chr5_2:73491700-73491719 Msa0787580:CDS 25.0%
!!! CAATTTACCGTTTTAATCAA+GGG - chr5_2:73491701-73491720 Msa0787580:CDS 25.0%
! TTCAATGAAATCACAAATGT+TGG - chr5_2:73491860-73491879 Msa0787580:intron 25.0%
!! AAGTGAAACTGATTCTTATA+TGG - chr5_2:73491967-73491986 Msa0787580:intron 25.0%
!!! ATTGTTGTTTGTTTGGAATT+AGG - chr5_2:73492021-73492040 Msa0787580:intron 25.0%
!!! TTGTTGTTTGTTTGGAATTA+GGG - chr5_2:73492022-73492041 Msa0787580:intron 25.0%
!! GTTAACAAGAAATAGCATTT+TGG - chr5_2:73492313-73492332 Msa0787580:intron 25.0%
!! TGAAAGTATAGACATAATCA+GGG + chr5_2:73492916-73492935 None:intergenic 25.0%
!! ATGAAAGTATAGACATAATC+AGG + chr5_2:73492917-73492936 None:intergenic 25.0%
! TATAAAGAAAGAAGGGTAAA+GGG - chr5_2:73493069-73493088 Msa0787580:intron 25.0%
! GACTATATTACAGCTATTTA+AGG + chr5_2:73493158-73493177 None:intergenic 25.0%
!! TTTTCAAAATTGAAGTAGTC+CGG - chr5_2:73493350-73493369 Msa0787580:CDS 25.0%
!!! AATTTTTTATAAACCAGACC+CGG + chr5_2:73493372-73493391 None:intergenic 25.0%
!! GTCTGGTTTATAAAAAATTC+AGG - chr5_2:73493373-73493392 Msa0787580:CDS 25.0%
!! CTCTATCTACGATAATTTTT+AGG - chr5_2:73493745-73493764 Msa0787580:intron 25.0%
! ATCAATGATTAGAACTTAGA+AGG + chr5_2:73494117-73494136 None:intergenic 25.0%
!!! GTTTCATTTTTGTACTTGTT+TGG - chr5_2:73494179-73494198 Msa0787580:intron 25.0%
!! GCATTTTCTTTTATTATGGA+AGG - chr5_2:73494274-73494293 Msa0787580:intron 25.0%
! AAAGCTTCTTTCAATTCATT+TGG + chr5_2:73494403-73494422 None:intergenic 25.0%
! TACTTCACATAAGTGTATTT+CGG + chr5_2:73494744-73494763 None:intergenic 25.0%
AATTGAAAGAGATAACGCAT+AGG - chr5_2:73491260-73491279 Msa0787580:three_prime_UTR 30.0%
AGAAAAACCGTATCCAAAAA+CGG + chr5_2:73491506-73491525 None:intergenic 30.0%
CACTAAACCCTTGATTAAAA+CGG + chr5_2:73491711-73491730 None:intergenic 30.0%
! ATACATTGATTGTCAATGCT+TGG - chr5_2:73491746-73491765 Msa0787580:intron 30.0%
ACTAACAACAATAAGCTTTG+TGG + chr5_2:73491940-73491959 None:intergenic 30.0%
!!! ACTATGTATGTGTTTTTGGT+TGG + chr5_2:73492195-73492214 None:intergenic 30.0%
!!! TTATTTTGGAAGTGACAAGA+TGG - chr5_2:73492215-73492234 Msa0787580:intron 30.0%
CATCTTAGTCAGAAAATAAG+AGG + chr5_2:73492458-73492477 None:intergenic 30.0%
! TTTTTGATACCTCAATTAGC+TGG - chr5_2:73492511-73492530 Msa0787580:intron 30.0%
AAACTGCAAATGTCTAACAA+AGG + chr5_2:73492780-73492799 None:intergenic 30.0%
! GAAAGTATAGACATAATCAG+GGG + chr5_2:73492915-73492934 None:intergenic 30.0%
TGGAACAAGTTTCAAAGAAA+GGG + chr5_2:73493038-73493057 None:intergenic 30.0%
TCTTTCTTTATACGCCAATA+AGG + chr5_2:73493061-73493080 None:intergenic 30.0%
TATTGGCGTATAAAGAAAGA+AGG - chr5_2:73493061-73493080 Msa0787580:intron 30.0%
ATTGGCGTATAAAGAAAGAA+GGG - chr5_2:73493062-73493081 Msa0787580:intron 30.0%
GTATAAAGAAAGAAGGGTAA+AGG - chr5_2:73493068-73493087 Msa0787580:intron 30.0%
TAATATAGTCCCTTATTGGT+GGG - chr5_2:73493168-73493187 Msa0787580:CDS 30.0%
TTTCAAAATTGAAGTAGTCC+GGG - chr5_2:73493351-73493370 Msa0787580:CDS 30.0%
! ACTTTTGGATTCCTTAACAA+GGG + chr5_2:73493787-73493806 None:intergenic 30.0%
! TACTTTTGGATTCCTTAACA+AGG + chr5_2:73493788-73493807 None:intergenic 30.0%
CTTATCATCTAGTATTCTTC+AGG - chr5_2:73494211-73494230 Msa0787580:intron 30.0%
!! TGGGGCATTTTCTTTTATTA+TGG - chr5_2:73494270-73494289 Msa0787580:intron 30.0%
CTTTCAATTCATTTGGAAGA+GGG + chr5_2:73494396-73494415 None:intergenic 30.0%
TCTTTCAATTCATTTGGAAG+AGG + chr5_2:73494397-73494416 None:intergenic 30.0%
!!! AGAAGCTTTCTCTTTTTTCT+CGG - chr5_2:73494414-73494433 Msa0787580:intron 30.0%
AAATGCTGATAAAAACTGCA+AGG + chr5_2:73494559-73494578 None:intergenic 30.0%
!! CAACATATCATTGCTTTTCT+CGG - chr5_2:73494652-73494671 Msa0787580:exon 30.0%
AATACACTTATGTGAAGTAG+TGG - chr5_2:73494745-73494764 Msa0787580:five_prime_UTR 30.0%
ACTGAAGTGAGTAACAGTTA+TGG - chr5_2:73491170-73491189 Msa0787580:three_prime_UTR 35.0%
ATTGTCAATGCTTGGAATAC+TGG - chr5_2:73491754-73491773 Msa0787580:intron 35.0%
TATGAAACACAGACACGAAA+CGG - chr5_2:73491798-73491817 Msa0787580:intron 35.0%
AAATGAACTGAAGTTGTGCT+TGG + chr5_2:73492102-73492121 None:intergenic 35.0%
CAACAACAACAACAACAACA+AGG + chr5_2:73492430-73492449 None:intergenic 35.0%
ATGCTTAAGGATGCTTGTAA+TGG - chr5_2:73492550-73492569 Msa0787580:intron 35.0%
TGCTTAAGGATGCTTGTAAT+GGG - chr5_2:73492551-73492570 Msa0787580:intron 35.0%
GCTGGATTGTTTAAGAAGAA+AGG - chr5_2:73492586-73492605 Msa0787580:intron 35.0%
!! TAGACATTTGCAGTTTTGTC+TGG - chr5_2:73492784-73492803 Msa0787580:intron 35.0%
! TTTGTCTGGAGTTCATAGTT+TGG - chr5_2:73492798-73492817 Msa0787580:intron 35.0%
ATATATGCAGTCATTAACGC+TGG - chr5_2:73492935-73492954 Msa0787580:intron 35.0%
GTGGAACAAGTTTCAAAGAA+AGG + chr5_2:73493039-73493058 None:intergenic 35.0%
! TGAAACTTGTTCCACCTTAT+TGG - chr5_2:73493044-73493063 Msa0787580:intron 35.0%
TTCTTTATACGCCAATAAGG+TGG + chr5_2:73493058-73493077 None:intergenic 35.0%
GCTGTAATATAGTCCCTTAT+TGG - chr5_2:73493164-73493183 Msa0787580:CDS 35.0%
GTAATATAGTCCCTTATTGG+TGG - chr5_2:73493167-73493186 Msa0787580:CDS 35.0%
!!! TTTTTAGTTGTATTCCACCG+TGG - chr5_2:73493200-73493219 Msa0787580:intron 35.0%
ACTTCTTGAATCCTAGAATC+AGG + chr5_2:73493274-73493293 None:intergenic 35.0%
GCATGAAGTAACAAAAACAG+AGG + chr5_2:73493566-73493585 None:intergenic 35.0%
TTCAACCTCTGTTCTGTAAA+TGG - chr5_2:73493605-73493624 Msa0787580:intron 35.0%
! CACTGCACAATTTCTACTTT+TGG + chr5_2:73493802-73493821 None:intergenic 35.0%
TTCCTTAACATGTACCCTTA+GGG - chr5_2:73493891-73493910 Msa0787580:intron 35.0%
AGTATACCATTTCGTACTAG+TGG - chr5_2:73493960-73493979 Msa0787580:intron 35.0%
GTATACCATTTCGTACTAGT+GGG - chr5_2:73493961-73493980 Msa0787580:intron 35.0%
GGTTAAAAGATCGTAACTCT+AGG + chr5_2:73493997-73494016 None:intergenic 35.0%
TTAAGAGTCTACGCAAGATT+GGG + chr5_2:73494024-73494043 None:intergenic 35.0%
ATTAAGAGTCTACGCAAGAT+TGG + chr5_2:73494025-73494044 None:intergenic 35.0%
!!! CTTTTTTTCCCTTTGCTTGA+CGG + chr5_2:73494067-73494086 None:intergenic 35.0%
TACATCTCGATAAGACATCT+GGG - chr5_2:73494471-73494490 Msa0787580:intron 35.0%
ACCTAAATAAAAGCGAAGGA+GGG + chr5_2:73494535-73494554 None:intergenic 35.0%
AACCTAAATAAAAGCGAAGG+AGG + chr5_2:73494536-73494555 None:intergenic 35.0%
AGGAACCTAAATAAAAGCGA+AGG + chr5_2:73494539-73494558 None:intergenic 35.0%
TGACGAAACATTGTTATCCT+AGG - chr5_2:73494600-73494619 Msa0787580:CDS 35.0%
CGAGAAAAGCAATGATATGT+TGG + chr5_2:73494654-73494673 None:intergenic 35.0%
GCGAATAGTTTGAACTTGAA+AGG - chr5_2:73494674-73494693 Msa0787580:five_prime_UTR 35.0%
ACTGTTACTCACTTCAGTCT+TGG + chr5_2:73491168-73491187 None:intergenic 40.0%
GAAAGAGATAACGCATAGGT+AGG - chr5_2:73491264-73491283 Msa0787580:three_prime_UTR 40.0%
!!! CTCTACTACTGTTGCTGTTT+TGG + chr5_2:73491321-73491340 None:intergenic 40.0%
AAACGGATAAGACAAACGGA+GGG + chr5_2:73491448-73491467 None:intergenic 40.0%
!!! GCTTTAATGGGTTCCGTTTT+TGG - chr5_2:73491490-73491509 Msa0787580:three_prime_UTR 40.0%
!! ATGGGTTCCGTTTTTGGATA+CGG - chr5_2:73491496-73491515 Msa0787580:exon 40.0%
AAATGTTGGTGTGTCCATGT+CGG - chr5_2:73491874-73491893 Msa0787580:intron 40.0%
! CTTCTGATCAAAAGTGTGTC+CGG + chr5_2:73491903-73491922 None:intergenic 40.0%
AGAAAAACGCCAGCTAATTG+AGG + chr5_2:73492523-73492542 None:intergenic 40.0%
TAAAGGATCCTTTGCAGATG+CGG - chr5_2:73492612-73492631 Msa0787580:intron 40.0%
AAGTCGACATCAAGCAATCA+AGG + chr5_2:73492679-73492698 None:intergenic 40.0%
TGCATCTTGAAAAGACTTCG+AGG - chr5_2:73492826-73492845 Msa0787580:intron 40.0%
CTTGAAAAGACTTCGAGGAA+AGG - chr5_2:73492831-73492850 Msa0787580:intron 40.0%
!! AAAGGCACTTCAGCAAGTTA+AGG + chr5_2:73493297-73493316 None:intergenic 40.0%
!! TTAACTTGCTGAAGTGCCTT+TGG - chr5_2:73493296-73493315 Msa0787580:intron 40.0%
ATCTCACCATTGTGATCCAA+AGG + chr5_2:73493315-73493334 None:intergenic 40.0%
TTGCTAGTGGAAAAACGAAG+CGG + chr5_2:73493494-73493513 None:intergenic 40.0%
ACAGAGAAACGAGGAAAAGT+GGG + chr5_2:73493528-73493547 None:intergenic 40.0%
AACAGAGAAACGAGGAAAAG+TGG + chr5_2:73493529-73493548 None:intergenic 40.0%
ATGCAAGAAACAGAGAAACG+AGG + chr5_2:73493537-73493556 None:intergenic 40.0%
! GTTTCTCTGTTTCTTGCATG+TGG - chr5_2:73493538-73493557 Msa0787580:intron 40.0%
TGAAGCCATTTACAGAACAG+AGG + chr5_2:73493613-73493632 None:intergenic 40.0%
GAGTAATGGTTATGAACCTG+TGG - chr5_2:73493675-73493694 Msa0787580:intron 40.0%
TGGTTATGAACCTGTGGATT+TGG - chr5_2:73493681-73493700 Msa0787580:intron 40.0%
GTTCCTTAACATGTACCCTT+AGG - chr5_2:73493890-73493909 Msa0787580:intron 40.0%
AATGCTAATGTGTGCCCTAA+GGG + chr5_2:73493908-73493927 None:intergenic 40.0%
AAATGCTAATGTGTGCCCTA+AGG + chr5_2:73493909-73493928 None:intergenic 40.0%
AAGATCCCACTAGTACGAAA+TGG + chr5_2:73493969-73493988 None:intergenic 40.0%
CGTCAAGCAAAGGGAAAAAA+AGG - chr5_2:73494065-73494084 Msa0787580:intron 40.0%
AAGAAAATGCCCCAAAAGTG+AGG + chr5_2:73494264-73494283 None:intergenic 40.0%
ATTTGGAAGAGGGAGTTGAA+GGG + chr5_2:73494386-73494405 None:intergenic 40.0%
! TCTTTGAAAAAGCGCAGCAA+GGG - chr5_2:73494439-73494458 Msa0787580:intron 40.0%
CAGATGTCTTATCGAGATGT+AGG + chr5_2:73494472-73494491 None:intergenic 40.0%
CTACATCTCGATAAGACATC+TGG - chr5_2:73494470-73494489 Msa0787580:intron 40.0%
GGGTGCATATCTTATCTACA+TGG + chr5_2:73494515-73494534 None:intergenic 40.0%
!! ACCCTCCTTCGCTTTTATTT+AGG - chr5_2:73494531-73494550 Msa0787580:CDS 40.0%
ATGTCAACAACACATCTCCT+AGG + chr5_2:73494620-73494639 None:intergenic 40.0%
TTATGTGAAGTAGTGGCAGT+CGG - chr5_2:73494752-73494771 Msa0787580:five_prime_UTR 40.0%
CAGCAACAGTAGTAGAGCAA+TGG - chr5_2:73491324-73491343 Msa0787580:three_prime_UTR 45.0%
GAAACGGATAAGACAAACGG+AGG + chr5_2:73491449-73491468 None:intergenic 45.0%
GGCGAAACGGATAAGACAAA+CGG + chr5_2:73491452-73491471 None:intergenic 45.0%
! AAGGCCTTATCCCACTTAGT+GGG + chr5_2:73492411-73492430 None:intergenic 45.0%
!! GCTTGTAATGGGTGTGTTTC+AGG - chr5_2:73492562-73492581 Msa0787580:intron 45.0%
AAAGGATCCTTTGCAGATGC+GGG - chr5_2:73492613-73492632 Msa0787580:intron 45.0%
AGACTTCGAGGAAAGGATGA+TGG - chr5_2:73492838-73492857 Msa0787580:intron 45.0%
!! GGACTTGCTCTAAGTTTTCC+CGG - chr5_2:73492977-73492996 Msa0787580:intron 45.0%
ATAACCTCGCCCACCAATAA+GGG + chr5_2:73493180-73493199 None:intergenic 45.0%
AATAACCTCGCCCACCAATA+AGG + chr5_2:73493181-73493200 None:intergenic 45.0%
! GAAGTGCCTTTGGATCACAA+TGG - chr5_2:73493306-73493325 Msa0787580:intron 45.0%
AAATTGAAGTAGTCCGGGTC+TGG - chr5_2:73493356-73493375 Msa0787580:CDS 45.0%
TGTTCATAGGCCAAATCCAC+AGG + chr5_2:73493694-73493713 None:intergenic 45.0%
! TCCTTAACAAGGGCACACAT+TGG + chr5_2:73493777-73493796 None:intergenic 45.0%
! TGCCCTAAGGGTACATGTTA+AGG + chr5_2:73493896-73493915 None:intergenic 45.0%
TTGTCATCACCGTCAAGCAA+AGG - chr5_2:73494055-73494074 Msa0787580:intron 45.0%
TGTCATCACCGTCAAGCAAA+GGG - chr5_2:73494056-73494075 Msa0787580:intron 45.0%
TTTGGAAGAGGGAGTTGAAG+GGG + chr5_2:73494385-73494404 None:intergenic 45.0%
CATTTGGAAGAGGGAGTTGA+AGG + chr5_2:73494387-73494406 None:intergenic 45.0%
! GTCTTTGAAAAAGCGCAGCA+AGG - chr5_2:73494438-73494457 Msa0787580:intron 45.0%
CAGTAGTAGAGCAATGGCGA+CGG - chr5_2:73491330-73491349 Msa0787580:three_prime_UTR 50.0%
GAGGAATCTTCGTCATGAGG+CGG + chr5_2:73491376-73491395 None:intergenic 50.0%
TCGGAGGAATCTTCGTCATG+AGG + chr5_2:73491379-73491398 None:intergenic 50.0%
AGAATTGGGAACGGAGGAGT+CGG + chr5_2:73491398-73491417 None:intergenic 50.0%
! GGTGTTGGGAGAATTGGGAA+CGG + chr5_2:73491407-73491426 None:intergenic 50.0%
! ATGGCGGTGTTGGGAGAATT+GGG + chr5_2:73491412-73491431 None:intergenic 50.0%
GATAAGACAAACGGAGGGAG+CGG + chr5_2:73491443-73491462 None:intergenic 50.0%
ACTTAGTGGGGTAGGCTACA+TGG + chr5_2:73492398-73492417 None:intergenic 50.0%
ATGTAGCCTACCCCACTAAG+TGG - chr5_2:73492397-73492416 Msa0787580:intron 50.0%
TGTAGCCTACCCCACTAAGT+GGG - chr5_2:73492398-73492417 Msa0787580:intron 50.0%
CTTATCCCACTTAGTGGGGT+AGG + chr5_2:73492406-73492425 None:intergenic 50.0%
CTACCCCACTAAGTGGGATA+AGG - chr5_2:73492404-73492423 Msa0787580:intron 50.0%
! AGGCCTTATCCCACTTAGTG+GGG + chr5_2:73492410-73492429 None:intergenic 50.0%
! CAAGGCCTTATCCCACTTAG+TGG + chr5_2:73492412-73492431 None:intergenic 50.0%
! AATGGGTGTGTTTCAGGTGC+TGG - chr5_2:73492568-73492587 Msa0787580:intron 50.0%
AAGGATCCTTTGCAGATGCG+GGG - chr5_2:73492614-73492633 Msa0787580:intron 50.0%
GTCATTAACGCTGGAGTAGC+TGG - chr5_2:73492944-73492963 Msa0787580:intron 50.0%
TGGAGTAGCTGGATGTTGCA+CGG - chr5_2:73492955-73492974 Msa0787580:intron 50.0%
!! ACACTGGATGGTGGATTCCA+CGG + chr5_2:73493220-73493239 None:intergenic 50.0%
TGGAATCCACCATCCAGTGT+TGG - chr5_2:73493220-73493239 Msa0787580:intron 50.0%
! AATCCACCATCCAGTGTTGG+TGG - chr5_2:73493223-73493242 Msa0787580:intron 50.0%
ATTCCACCAACACTGGATGG+TGG + chr5_2:73493229-73493248 None:intergenic 50.0%
GAGATTCCACCAACACTGGA+TGG + chr5_2:73493232-73493251 None:intergenic 50.0%
ACTGGAGATTCCACCAACAC+TGG + chr5_2:73493236-73493255 None:intergenic 50.0%
GGAATCTCCAGTTGAAGCCT+AGG - chr5_2:73493244-73493263 Msa0787580:intron 50.0%
TCCTAGAATCAGGCGATCCT+AGG + chr5_2:73493264-73493283 None:intergenic 50.0%
GGAGGAGGTAGTATTGCTAG+TGG + chr5_2:73493507-73493526 None:intergenic 50.0%
AAACGAGGAAAAGTGGGAGG+AGG + chr5_2:73493522-73493541 None:intergenic 50.0%
GAGAAACGAGGAAAAGTGGG+AGG + chr5_2:73493525-73493544 None:intergenic 50.0%
GCCAATGTGTGCCCTTGTTA+AGG - chr5_2:73493773-73493792 Msa0787580:intron 50.0%
AGTCTACGCAAGATTGGGAG+GGG + chr5_2:73494019-73494038 None:intergenic 50.0%
GAGTCTACGCAAGATTGGGA+GGG + chr5_2:73494020-73494039 None:intergenic 50.0%
AGAGTCTACGCAAGATTGGG+AGG + chr5_2:73494021-73494040 None:intergenic 50.0%
!! TGCAGGGTTGCCTCACTTTT+GGG - chr5_2:73494251-73494270 Msa0787580:intron 50.0%
TGCCCATGTCCTGGAAAACA+GGG - chr5_2:73494326-73494345 Msa0787580:intron 50.0%
! AGCCCTGTTTTCCAGGACAT+GGG + chr5_2:73494331-73494350 None:intergenic 50.0%
! AGTTGAAGGGGGAGTACAAG+GGG + chr5_2:73494373-73494392 None:intergenic 50.0%
! GAGTTGAAGGGGGAGTACAA+GGG + chr5_2:73494374-73494393 None:intergenic 50.0%
TTGGAAGAGGGAGTTGAAGG+GGG + chr5_2:73494384-73494403 None:intergenic 50.0%
TAGTAGAGCAATGGCGACGG+CGG - chr5_2:73491333-73491352 Msa0787580:three_prime_UTR 55.0%
ATGAGGCGGTGTGATACCGT+CGG + chr5_2:73491362-73491381 None:intergenic 55.0%
GTTGGGAGAATTGGGAACGG+AGG + chr5_2:73491404-73491423 None:intergenic 55.0%
! GATGGCGGTGTTGGGAGAAT+TGG + chr5_2:73491413-73491432 None:intergenic 55.0%
! GGAACCCATTAAAGCGCCAC+CGG + chr5_2:73491485-73491504 None:intergenic 55.0%
AGAAGACCCCGCATCTGCAA+AGG + chr5_2:73492623-73492642 None:intergenic 55.0%
AGATGCGGGGTCTTCTGCAA+AGG - chr5_2:73492627-73492646 Msa0787580:intron 55.0%
GGAGTAGCTGGATGTTGCAC+GGG - chr5_2:73492956-73492975 Msa0787580:intron 55.0%
! TAGTCCCTTATTGGTGGGCG+AGG - chr5_2:73493173-73493192 Msa0787580:CDS 55.0%
AGGCGATCCTAGGCTTCAAC+TGG + chr5_2:73493254-73493273 None:intergenic 55.0%
GCCTAGGATCGCCTGATTCT+AGG - chr5_2:73493260-73493279 Msa0787580:intron 55.0%
GTCTACGCAAGATTGGGAGG+GGG + chr5_2:73494018-73494037 None:intergenic 55.0%
! CTGCAGGGTTGCCTCACTTT+TGG - chr5_2:73494250-73494269 Msa0787580:intron 55.0%
!! GCAGGGTTGCCTCACTTTTG+GGG - chr5_2:73494252-73494271 Msa0787580:intron 55.0%
CTGCCCATGTCCTGGAAAAC+AGG - chr5_2:73494325-73494344 Msa0787580:intron 55.0%
! CAGCCCTGTTTTCCAGGACA+TGG + chr5_2:73494332-73494351 None:intergenic 55.0%
! GGAGTTGAAGGGGGAGTACA+AGG + chr5_2:73494375-73494394 None:intergenic 55.0%
ATTGGGAACGGAGGAGTCGG+AGG + chr5_2:73491395-73491414 None:intergenic 60.0%
AAGACAAACGGAGGGAGCGG+CGG + chr5_2:73491440-73491459 None:intergenic 60.0%
CGGCGGAATCAGTGGCGAAA+CGG + chr5_2:73491465-73491484 None:intergenic 60.0%
!! CGTTTCGCCACTGATTCCGC+CGG - chr5_2:73491463-73491482 Msa0787580:three_prime_UTR 60.0%
TTCCGCCGGTGGCGCTTTAA+TGG - chr5_2:73491477-73491496 Msa0787580:three_prime_UTR 60.0%
TCCGCCGGTGGCGCTTTAAT+GGG - chr5_2:73491478-73491497 Msa0787580:three_prime_UTR 60.0%
! ACCCATTAAAGCGCCACCGG+CGG + chr5_2:73491482-73491501 None:intergenic 60.0%
GGTGTGTCCATGTCGGACAC+CGG - chr5_2:73491881-73491900 Msa0787580:intron 60.0%
AGTGTGTCCGGTGTCCGACA+TGG + chr5_2:73491891-73491910 None:intergenic 60.0%
!! CTGGATGGTGGATTCCACGG+TGG + chr5_2:73493217-73493236 None:intergenic 60.0%
ATCGCTGTCGCACAGGGAAG+AGG + chr5_2:73493441-73493460 None:intergenic 60.0%
GGTAGCATCGCTGTCGCACA+GGG + chr5_2:73493447-73493466 None:intergenic 60.0%
AGGTAGCATCGCTGTCGCAC+AGG + chr5_2:73493448-73493467 None:intergenic 60.0%
TGCTACCTCTCCTCCACCAG+CGG - chr5_2:73493460-73493479 Msa0787580:intron 60.0%
TGCACCACAAGCGCTTCTGC+AGG - chr5_2:73494234-73494253 Msa0787580:intron 60.0%
GCACCACAAGCGCTTCTGCA+GGG - chr5_2:73494235-73494254 Msa0787580:intron 60.0%
!! CAACCCTGCAGAAGCGCTTG+TGG + chr5_2:73494241-73494260 None:intergenic 60.0%
GACATGGGCAGTGCAAGTGC+TGG + chr5_2:73494316-73494335 None:intergenic 60.0%
CACTTGCACTGCCCATGTCC+TGG - chr5_2:73494317-73494336 Msa0787580:intron 60.0%
! GTGCTGCAGCCCTGTTTTCC+AGG + chr5_2:73494338-73494357 None:intergenic 60.0%
! TGAAGGGGGAGTACAAGGGG+AGG + chr5_2:73494370-73494389 None:intergenic 60.0%
! ATGGCGACGGCGGATTCCGA+CGG - chr5_2:73491343-73491362 Msa0787580:three_prime_UTR 65.0%
! GCGGGAACGATGGCGGTGTT+GGG + chr5_2:73491421-73491440 None:intergenic 65.0%
AGACAAACGGAGGGAGCGGC+GGG + chr5_2:73491439-73491458 None:intergenic 65.0%
!! TTCGCCACTGATTCCGCCGG+TGG - chr5_2:73491466-73491485 Msa0787580:three_prime_UTR 65.0%
! GGCGGGAACGATGGCGGTGT+TGG + chr5_2:73491422-73491441 None:intergenic 70.0%
AGCGCCACCGGCGGAATCAG+TGG + chr5_2:73491473-73491492 None:intergenic 70.0%
GGGAGCGGCGGGAACGATGG+CGG + chr5_2:73491428-73491447 None:intergenic 75.0%
GGAGGGAGCGGCGGGAACGA+TGG + chr5_2:73491431-73491450 None:intergenic 75.0%
CGCAGCCGCTGGTGGAGGAG+AGG + chr5_2:73493468-73493487 None:intergenic 75.0%
! GGTGTCGCAGCCGCTGGTGG+AGG + chr5_2:73493473-73493492 None:intergenic 75.0%
!! AGCGGTGTCGCAGCCGCTGG+TGG + chr5_2:73493476-73493495 None:intergenic 75.0%
!! CGAAGCGGTGTCGCAGCCGC+TGG + chr5_2:73493479-73493498 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 73491165 73494830 73491165 ID=Msa0787580;Name=Msa0787580
chr5_2 mRNA 73491165 73494830 73491165 ID=Msa0787580-mRNA-1;Parent=Msa0787580;Name=Msa0787580-mRNA-1;_AED=0.09;_eAED=0.09;_QI=178|1|1|1|1|1|5|347|205
chr5_2 exon 73491165 73491762 73491165 ID=Msa0787580-mRNA-1:exon:22410;Parent=Msa0787580-mRNA-1
chr5_2 exon 73492997 73493050 73492997 ID=Msa0787580-mRNA-1:exon:22409;Parent=Msa0787580-mRNA-1
chr5_2 exon 73493136 73493208 73493136 ID=Msa0787580-mRNA-1:exon:22408;Parent=Msa0787580-mRNA-1
chr5_2 exon 73493347 73493411 73493347 ID=Msa0787580-mRNA-1:exon:22407;Parent=Msa0787580-mRNA-1
chr5_2 exon 73494478 73494830 73494478 ID=Msa0787580-mRNA-1:exon:22406;Parent=Msa0787580-mRNA-1
chr5_2 five_prime_UTR 73494653 73494830 73494653 ID=Msa0787580-mRNA-1:five_prime_utr;Parent=Msa0787580-mRNA-1
chr5_2 CDS 73494478 73494652 73494478 ID=Msa0787580-mRNA-1:cds;Parent=Msa0787580-mRNA-1
chr5_2 CDS 73493347 73493411 73493347 ID=Msa0787580-mRNA-1:cds;Parent=Msa0787580-mRNA-1
chr5_2 CDS 73493136 73493208 73493136 ID=Msa0787580-mRNA-1:cds;Parent=Msa0787580-mRNA-1
chr5_2 CDS 73492997 73493050 73492997 ID=Msa0787580-mRNA-1:cds;Parent=Msa0787580-mRNA-1
chr5_2 CDS 73491512 73491762 73491512 ID=Msa0787580-mRNA-1:cds;Parent=Msa0787580-mRNA-1
chr5_2 three_prime_UTR 73491165 73491511 73491165 ID=Msa0787580-mRNA-1:three_prime_utr;Parent=Msa0787580-mRNA-1
Gene Sequence

>Msa0787580

ATGGCGACGGCGGATTCCGACGGTATCACACCGCCTCATGACGAAGATTCCTCCGACTCCTCCGTTCCCAATTCTCCCAACACCGCCATCGTTCCCGCCGCTCCCTCCGTTTGTCTTATCCGTTTCGCCACTGATTCCGCCGGTGGCGCTTTAATGGGTTCCGTTTTTGGATACGGTGCTGGATTGTTTAAGAAGAAAGGTTTTAAAGGATCCTTTGCAGATGCGGGGTCTTCTGCAAAGACATTTGCAGTTTTGTCTGGAGTTCATAGTTTGGTTGTTTGCATCTTGAAAAGACTTCGAGGAAAGGATGATGTCATTAACGCTGGAGTAGCTGGATGTTGCACGGGACTTGCTCTAAGTTTTCCCGGTGCACCACAAGCGCTTCTGCAGGGTTGCCTCACTTTTGGGGCATTTTCTTTTATTATGGAAGGGCTAAACAAACAGCAGCCAGCACTTGCACTGCCCATGTCCTGGAAAACAGGGCTGCAGCACAATGCACGACCTCCCCTTGTACTCCCCCTTCAACTCCCTCTTCCAAATGAATTGAAAGAAGCTTTCTCTTTTTTCTCGGAGTCTTTGAAAAAGCGCAGCAAGGGAGCTTATCCTACATCTCGATAA
Protein sequence

>Msa0787580

MATADSDGITPPHDEDSSDSSVPNSPNTAIVPAAPSVCLIRFATDSAGGALMGSVFGYGAGLFKKKGFKGSFADAGSSAKTFAVLSGVHSLVVCILKRLRGKDDVINAGVAGCCTGLALSFPGAPQALLQGCLTFGAFSFIMEGLNKQQPALALPMSWKTGLQHNARPPLVLPLQLPLPNELKEAFSFFSESLKKRSKGAYPTSR*