AlfalfaGEDB | Msa0830300

M. sativa cultivar zhongmu-4 / Msa0830300

KEGG: -
GO: -
PFAM: PF12776.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830300	XP_039684367.1	88.435	294	21	1	308	588	50	343	0.0	548

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830300	A0A396GQG1	87.031	293	25	1	309	588	1	293	0.0	537

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0830300	Msa1429670	0.943861	5.595601e-103	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830300	MtrunA17_Chr8g0360271	87.031	293	25	1	309	588	1	293	0.0	537
Msa0830300	MtrunA17_Chr1g0209621	95.720	257	9	2	1	256	1	256	5.27e-156	449
Msa0830300	MtrunA17_Chr6g0484211	36.330	267	119	8	5	256	1	231	1.94e-23	99.8
Msa0830300	MtrunA17_Chr6g0457701	36.981	265	124	12	14	261	7	245	4.06e-23	99.0
Msa0830300	MtrunA17_Chr3g0110301	67.692	65	8	1	366	417	26	90	6.56e-23	94.0
Msa0830300	MtrunA17_Chr7g0250821	36.398	261	128	11	1	243	1	241	4.64e-22	96.3
Msa0830300	MtrunA17_Chr3g0084111	36.196	163	67	4	427	588	5	131	1.84e-19	85.1
Msa0830300	MtrunA17_Chr8g0391091	33.852	257	121	9	5	256	1	213	7.40e-15	74.3
Msa0830300	MtrunA17_Chr1g0156481	25.424	295	166	12	321	584	18	289	4.59e-11	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0830300	AT1G68330.1	42.143	280	130	11	1	262	1	266	1.40e-32	127
Msa0830300	AT1G48780.1	35.317	252	123	11	17	256	9	232	5.22e-17	81.6
Msa0830300	AT1G67050.1	36.331	278	129	12	1	256	1	252	3.83e-14	73.6
Msa0830300	AT3G18300.2	30.605	281	148	12	5	256	2	264	7.11e-13	69.7
Msa0830300	AT3G18300.1	30.605	281	148	12	5	256	2	264	7.11e-13	69.7

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Find 97 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTGAGGCGGTGAACG+AGG	0.615754	5_4:+3360110	Msa0830300:intron
AAAACTTTCGTTACAAAATC+AGG	0.317317	5_4:-3358600	None:intergenic
AAAAGCGTTGGAAAAGTTTA+AGG	0.170316	5_4:+3359755	Msa0830300:CDS
AAATTCTAGGACTGAATCCT+GGG	0.562407	5_4:-3355679	None:intergenic
AACGAGGTTCCTAACCTTGA+AGG	0.452298	5_4:+3360126	Msa0830300:CDS
AACTAAAGGCAAAGATGCAT+AGG	0.582306	5_4:+3358748	Msa0830300:CDS
AAGAAGAAGCAAGGTGTAGA+TGG	0.344753	5_4:-3357212	None:intergenic
AAGAAGGGATAGGAGTGCAG+AGG	0.647414	5_4:+3359644	Msa0830300:CDS
AAGAGCTGATAGGTTAAGAA+GGG	0.537119	5_4:+3359629	Msa0830300:CDS
AAGTGTGTGGCTGCTCTCGA+TGG	0.472530	5_4:+3359702	Msa0830300:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCATGTGAGAATGAAATTCT+AGG	-	chr5_4:3355695-3355714	None:intergenic	30.0%
AAACTACCGTAAAGAAAAAG+AGG	+	chr5_4:3355969-3355988	Msa0830300:CDS	30.0%
AGAATTCTTGTCTGATGAAA+CGG	+	chr5_4:3355997-3356016	Msa0830300:CDS	30.0%
CAGAAATGTTTGGATAACAA+TGG	-	chr5_4:3356040-3356059	None:intergenic	30.0%
CATTGTTATCCAAACATTTC+TGG	+	chr5_4:3356038-3356057	Msa0830300:CDS	30.0%
TTTGAATTGCCAGAAATGTT+TGG	-	chr5_4:3356050-3356069	None:intergenic	30.0%
GTTCTCTACAATTCTTATCA+AGG	+	chr5_4:3356119-3356138	Msa0830300:CDS	30.0%
AAGTTACAACACAATACTTG+AGG	+	chr5_4:3356737-3356756	Msa0830300:intron	30.0%
TGTAGATGGAAGAAAAATCA+CGG	-	chr5_4:3357201-3357220	None:intergenic	30.0%
GATTGATATTTGCAGCTTAT+AGG	+	chr5_4:3357551-3357570	Msa0830300:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	3355647	3360260	3355647	ID=Msa0830300;Name=Msa0830300
chr5_4	mRNA	3355647	3360260	3355647	ID=Msa0830300-mRNA-1;Parent=Msa0830300;Name=Msa0830300-mRNA-1;_AED=0.30;_eAED=0.35;_QI=0\|0.25\|0\|1\|1\|1\|5\|0\|632
chr5_4	exon	3355647	3356413	3355647	ID=Msa0830300-mRNA-1:exon:1243;Parent=Msa0830300-mRNA-1
chr5_4	exon	3357130	3357286	3357130	ID=Msa0830300-mRNA-1:exon:1244;Parent=Msa0830300-mRNA-1
chr5_4	exon	3358561	3358845	3358561	ID=Msa0830300-mRNA-1:exon:1245;Parent=Msa0830300-mRNA-1
chr5_4	exon	3359285	3359833	3359285	ID=Msa0830300-mRNA-1:exon:1246;Parent=Msa0830300-mRNA-1
chr5_4	exon	3360120	3360260	3360120	ID=Msa0830300-mRNA-1:exon:1247;Parent=Msa0830300-mRNA-1
chr5_4	CDS	3355647	3356413	3355647	ID=Msa0830300-mRNA-1:cds;Parent=Msa0830300-mRNA-1
chr5_4	CDS	3357130	3357286	3357130	ID=Msa0830300-mRNA-1:cds;Parent=Msa0830300-mRNA-1
chr5_4	CDS	3358561	3358845	3358561	ID=Msa0830300-mRNA-1:cds;Parent=Msa0830300-mRNA-1
chr5_4	CDS	3359285	3359833	3359285	ID=Msa0830300-mRNA-1:cds;Parent=Msa0830300-mRNA-1
chr5_4	CDS	3360120	3360260	3360120	ID=Msa0830300-mRNA-1:cds;Parent=Msa0830300-mRNA-1

Gene Sequence

>Msa0830300

ATGGCAATTGAAGTGTGTAGTGAAACAAACAGCCCAGGATTCAGTCCTAGAATTTCATTCTCACATGATTTAAAGAATTCAAATGGTTCAGAAATCCCCGTAGAAGATCTTCAGCTTCGGTCAGATTTACACCTCTTAGATTCAACCTCCGATTTTGTTTTCTCCATCGCCAATGGTTTTTCTCAGCAGTTCTCTTCAGCTGATGAACTTTTTTCCGATGGAAAAATAGTTCCAATGGAAATAAAAAAAGTTATCTCCAATGGAATTATCGTTCCAATGAAGAAAAATTCATCAGCTAATGAATTCTTCGAGCCTCTTCAAAAAACTACCGTAAAGAAAAAGAGGCTTAAAGAATTCTTGTCTGATGAAACGGACGACGAAGAAGAGAAGCCATTGTTATCCAAACATTTCTGGCAATTCAAAAGAAGCAGCAGTTTGAATTTCGATACAGCTCGTCAAAATAAATTGATTCGTTCTCTACAATTCTTATCAAGGAGCCATTCAACCGGTTCAGTGCTGAATCCAAAACAAACAGAGCTTCAAAAAGAAAGACAAAAGCAAAGGTTGCAGAAACAATCTTCTGTTTCAAGTAGTAGAAGATCTTCTTCGTCTTCTTCAACTTCAAGTACATATTATTTCTATAATTCATCTAATAAATCTTCGTTAAGGAAATGTTCAAGTAATGGTGTTAGAATTAGTCCTGTTTTGAATCTACCTCAAGCATATATTCCTAAAGTTACTGCTAAATTTTTTGGATTTGGTTCATTAAAAACTAACGTGAGAACACTGAGAAGCGAAAATGGCGATTTCGACGGCGATTCTTCTCCGATCTCCGCCGTGATTTTTCTTCCATCTACACCTTGCTTCTTCTTCTCCACCGCGCTTCTTCTCTACCGTGCTTCTTCTTCTCTGCGGTTTTTTCAGATGGCAACTAATGTTGACATTAATGAGACAAAGCTTTGGCCTGATTTTGTAACGAAAGTTTTCATTGACATAATGGTTGATGAAGTTACAAAAGGAAATATGCCAAATGGTGTGTTTCATACTAGAACATGGTCCTCAATGATGTATAAGTTGAGTACCATAACTAATCGACCGTATCAACCTGATCAACTAAAGGCAAAGATGCATAGGTTGCGAGCCATGTATCGTGAGTTCTATTCACTGAAGCATAACACCGGATTTGGCTGGAATGCTGAAACAAACGCTGTACATCCCAAAGCTGCAGAGTTTCAAAAGAAAGGGTGTGACCAATACAAGTTGCTGGAAATAATATTCAACAAAAACAATGCAACTGGAGTACTTCATCATTCATCTACCCAAGACCCACCAAACACAGATGACGAAAACGAGCTTGATGATCAATACATCAACAATGGGAATGGGAGTGCTAATCATGTGCATGTTGATAAGGATAGTTCATATGATGATCTACATGAAGTTGAGCACATCACTCGCAGTGAAAAGCGACAGGTTCATGTGAGATCCAAGAAGGAGTCTACATCACATCATATGAATGAGGCACTTGCAGCATGTGCTCAAGTTTCTTTGGTAAGAGCTGATAGGTTAAGAAGGGATAGGAGTGCAGAGGCTACTTCGCGTGTAACTTCAGATTGTTCTCTTACCAAGTGTGTGGCTGCTCTCGATGGGCTAGAAGACATTTCAGATGATTCCTATGGAAAAGCGTTGGAAAAGTTTAAGGATTTTGATTGGAGGGAAATGTTTATCGCCATGCCTAATGAGAGGAAGCGTGGATGGGCGGTGAACGAGGTTCCTAACCTTGAAGGGATGAGCTCGTATGTTGAAAACTTAACAAGGTTATCTGATGAAGGTGCAGCTGAGATGGCAATGGATAGGAATCATCTTAGAGATAGGATGTGGGTACATCGTCATAATTAA

Protein sequence

>Msa0830300

MAIEVCSETNSPGFSPRISFSHDLKNSNGSEIPVEDLQLRSDLHLLDSTSDFVFSIANGFSQQFSSADELFSDGKIVPMEIKKVISNGIIVPMKKNSSANEFFEPLQKTTVKKKRLKEFLSDETDDEEEKPLLSKHFWQFKRSSSLNFDTARQNKLIRSLQFLSRSHSTGSVLNPKQTELQKERQKQRLQKQSSVSSSRRSSSSSSTSSTYYFYNSSNKSSLRKCSSNGVRISPVLNLPQAYIPKVTAKFFGFGSLKTNVRTLRSENGDFDGDSSPISAVIFLPSTPCFFFSTALLLYRASSSLRFFQMATNVDINETKLWPDFVTKVFIDIMVDEVTKGNMPNGVFHTRTWSSMMYKLSTITNRPYQPDQLKAKMHRLRAMYREFYSLKHNTGFGWNAETNAVHPKAAEFQKKGCDQYKLLEIIFNKNNATGVLHHSSTQDPPNTDDENELDDQYINNGNGSANHVHVDKDSSYDDLHEVEHITRSEKRQVHVRSKKESTSHHMNEALAACAQVSLVRADRLRRDRSAEATSRVTSDCSLTKCVAALDGLEDISDDSYGKALEKFKDFDWREMFIAMPNERKRGWAVNEVPNLEGMSSYVENLTRLSDEGAAEMAMDRNHLRDRMWVHRHN*