AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0830340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0830340 MtrunA17_Chr1g0209591 92.887 239 17 0 1 239 1 239 1.16e-163 452
Msa0830340 MtrunA17_Chr6g0451821 60.630 254 73 9 1 238 1 243 1.73e-87 260
Msa0830340 MtrunA17_Chr5g0438001 64.173 254 65 9 1 238 1 244 3.78e-85 254
Msa0830340 MtrunA17_Chr1g0209581 59.389 229 66 8 1 211 1 220 2.25e-71 217
Msa0830340 MtrunA17_Chr6g0484191 36.364 253 99 9 20 234 2 230 3.16e-36 127
Msa0830340 MtrunA17_Chr5g0423311 35.169 236 121 7 25 238 25 250 9.54e-32 117
Msa0830340 MtrunA17_Chr4g0055861 32.653 245 108 8 26 230 11 238 7.07e-26 101
Msa0830340 MtrunA17_Chr5g0423331 35.714 168 67 7 77 230 73 213 1.38e-21 89.7
Msa0830340 MtrunA17_Chr5g0423281 33.333 213 105 10 28 230 17 202 2.47e-21 88.6
Msa0830340 MtrunA17_Chr8g0335091 35.484 124 58 3 119 236 445 552 1.78e-12 66.2
Msa0830340 MtrunA17_Chr8g0369051 45.614 57 31 0 172 228 171 227 2.06e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0830340 AT1G25370.2 34.532 278 119 10 1 230 1 263 2.20e-31 116
Msa0830340 AT1G25370.1 34.532 278 119 10 1 230 1 263 2.20e-31 116
Msa0830340 AT1G68340.2 32.000 250 122 8 17 230 2 239 5.72e-27 104
Msa0830340 AT1G68340.1 32.000 250 122 8 17 230 2 239 5.85e-27 104
Msa0830340 AT3G18295.1 41.014 217 101 8 28 232 13 214 7.16e-27 103
Msa0830340 AT3G60410.1 43.200 125 56 4 119 228 196 320 7.26e-21 89.7
Msa0830340 AT3G60410.2 43.200 125 56 4 119 228 196 320 7.26e-21 89.7
Msa0830340 AT3G60410.3 43.200 125 56 4 119 228 196 320 7.26e-21 89.7
Msa0830340 AT1G68340.3 31.169 231 111 8 17 211 2 220 1.05e-20 87.8
Msa0830340 AT1G48770.1 33.831 201 96 6 28 226 11 176 3.27e-15 71.6
Msa0830340 AT1G48770.2 33.831 201 96 6 28 226 11 176 3.27e-15 71.6
Msa0830340 AT1G48770.3 33.831 201 96 6 28 226 11 176 3.27e-15 71.6
Msa0830340 AT4G17440.1 33.071 127 64 3 123 228 83 209 4.74e-13 66.6
Msa0830340 AT4G17440.2 33.071 127 64 3 123 228 83 209 4.74e-13 66.6
Msa0830340 AT4G17440.3 33.071 127 64 3 123 228 83 209 5.79e-13 66.6
Msa0830340 AT4G17440.4 32.061 131 64 4 123 228 83 213 5.67e-11 60.8

Find 89 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCTTTCAATGTTGATTT+TGG 0.185271 5_4:-3370845 Msa0830340:three_prime_UTR
ATTTGCTTCTGAACATTTCT+AGG 0.189823 5_4:+3371299 None:intergenic
AGAAGAGAGTGGTGCTGTTA+TGG 0.254438 5_4:-3371882 Msa0830340:CDS
CCCACTTCAAATAAGGAAAA+TGG 0.259092 5_4:+3371810 None:intergenic
ATAATTCTCTTATGAGATAT+TGG 0.275131 5_4:-3370786 Msa0830340:three_prime_UTR
TAATTCTCTTATGAGATATT+GGG 0.311967 5_4:-3370785 Msa0830340:three_prime_UTR
CTCTCGGTTTGTCGCCGTTA+AGG 0.320855 5_4:-3371466 Msa0830340:CDS
CTATGATGTGGGATTTGATT+TGG 0.324597 5_4:-3370908 Msa0830340:three_prime_UTR
GAAGAGAGTGGTGCTGTTAT+GGG 0.326106 5_4:-3371881 Msa0830340:CDS
AACATCATCAGGAACCTTAT+AGG 0.333378 5_4:+3371131 None:intergenic
CAACTCGGAGAAATCTCAAT+AGG 0.347215 5_4:-3371984 Msa0830340:five_prime_UTR
GATTAATGCTGATTCCTATA+AGG 0.358878 5_4:-3371145 Msa0830340:CDS
AAAGAGAATGGTGGTGGAGA+AGG 0.384258 5_4:-3371590 Msa0830340:CDS
CTTCAATCTTTCACCTTCAC+CGG 0.392665 5_4:+3371850 None:intergenic
ACACAAATCAACTAAACTAC+TGG 0.402438 5_4:+3370873 None:intergenic
GTAAACTTCACCTTCTTCTC+AGG 0.423971 5_4:+3371404 None:intergenic
CCGCCGTTACCATTCCTTAA+CGG 0.425360 5_4:+3371452 None:intergenic
TATTCTTATAAACAAAAGAA+TGG 0.433263 5_4:+3370806 None:intergenic
GATGATGTTCCTGAGAATAA+TGG 0.435032 5_4:-3371117 Msa0830340:CDS
AACTCGGAGAAATCTCAATA+GGG 0.438602 5_4:-3371983 Msa0830340:five_prime_UTR
AAGAGAATGGTGGTGGAGAA+GGG 0.452542 5_4:-3371589 Msa0830340:CDS
TTGAAGAAGGAGAAAGAGAA+TGG 0.453989 5_4:-3371602 Msa0830340:CDS
TGGTGTCACCGCCGGAGAAT+AGG 0.459611 5_4:-3371730 Msa0830340:CDS
GGGTGGCTTGAAAACTCGCC+GGG 0.466575 5_4:+3372048 None:intergenic
GCTGTTATGGGTAATGGTTC+CGG 0.467366 5_4:-3371869 Msa0830340:CDS
TTATTCTCAGGAACATCATC+AGG 0.474050 5_4:+3371120 None:intergenic
CACACCTTGCCATTATTCTC+AGG 0.477305 5_4:+3371108 None:intergenic
AAGGAGAAAGAGAATGGTGG+TGG 0.478905 5_4:-3371596 Msa0830340:CDS
GAAGATTTCATCACGATGAC+TGG 0.482101 5_4:-3371353 Msa0830340:CDS
AGAATCGATGGTGGTGATGA+TGG 0.483584 5_4:-3371695 Msa0830340:CDS
ACAACCACATACCAGGGAAA+AGG 0.488217 5_4:+3371172 None:intergenic
TACTGTGTCATTGATGAAGA+AGG 0.488455 5_4:-3371384 Msa0830340:CDS
TAGAAATGTTCAGAAGCAAA+TGG 0.488562 5_4:-3371297 Msa0830340:CDS
CAACCACATACCAGGGAAAA+GGG 0.489538 5_4:+3371173 None:intergenic
GGGTCGTCGACGGCTGCGGC+GGG 0.492188 5_4:-3371569 Msa0830340:CDS
GGAAAATGGAAGTTGTGAAA+AGG 0.494064 5_4:+3371824 None:intergenic
AGAAGGGTCGTCGACGGCTG+CGG 0.494461 5_4:-3371573 Msa0830340:CDS
GCTAGGAGTGGTAACAGCTT+AGG 0.503173 5_4:-3370941 Msa0830340:three_prime_UTR
GACGGCTGCGGCGGGAGAGA+AGG 0.504619 5_4:-3371561 Msa0830340:CDS
ATCTCAGACAACCACATACC+AGG 0.508509 5_4:+3371165 None:intergenic
AAGGTTGATGTTTGATCTGA+AGG 0.512312 5_4:-3371654 Msa0830340:CDS
AGGGTCGTCGACGGCTGCGG+CGG 0.517125 5_4:-3371570 Msa0830340:CDS
AACGGCGGTGGTAAGTTAAG+AGG 0.518465 5_4:-3371437 Msa0830340:CDS
AGTGGTGCTGTTATGGGTAA+TGG 0.525672 5_4:-3371875 Msa0830340:CDS
AGGTGTGGAATTTGAGAACA+AGG 0.527843 5_4:-3371541 Msa0830340:CDS
GGTTTGTCGCCGTTAAGGAA+TGG 0.536117 5_4:-3371461 Msa0830340:CDS
AATTCTCTTATGAGATATTG+GGG 0.539956 5_4:-3370784 Msa0830340:three_prime_UTR
AAAGAATGGACTATTCCCTA+TGG 0.543621 5_4:+3370820 None:intergenic
AAGCAATGAGAATGACAACT+TGG 0.552322 5_4:-3370984 Msa0830340:three_prime_UTR
TGTCAGAAGAATCCTGAAAA+CGG 0.554244 5_4:-3371767 Msa0830340:CDS
GCCTGTGTTAACTGACTGCT+AGG 0.556311 5_4:-3370958 Msa0830340:three_prime_UTR
CTGGTCAAAAGCCTCATAGA+AGG 0.556564 5_4:-3371334 Msa0830340:CDS
GCTCAGAAAATTCTTCAACT+CGG 0.562918 5_4:-3371999 Msa0830340:five_prime_UTR
ATGGGTAATGGTTCCGGTGA+AGG 0.563977 5_4:-3371863 Msa0830340:CDS
TGGTGGTTGTCCTGAGAAGA+AGG 0.564497 5_4:-3371414 Msa0830340:CDS
AGTCTGTTCATCCTATTCTC+CGG 0.565451 5_4:+3371719 None:intergenic
CACAAATCAACTAAACTACT+GGG 0.566998 5_4:+3370874 None:intergenic
TGGGTGGCTTGAAAACTCGC+CGG 0.570214 5_4:+3372047 None:intergenic
TTAAGGAATGGTAACGGCGG+TGG 0.572770 5_4:-3371449 Msa0830340:CDS
GGATACATCTGACTATGATG+TGG 0.574402 5_4:-3370920 Msa0830340:three_prime_UTR
TCCGTCGATGGTGTCACCGC+CGG 0.576373 5_4:-3371738 Msa0830340:CDS
AATGAGAATGACAACTTGGA+AGG 0.579939 5_4:-3370980 Msa0830340:three_prime_UTR
GGTGGTAAGTTAAGAGGTGG+TGG 0.580505 5_4:-3371431 Msa0830340:CDS
GGATCGATGCTATGCGTGAA+AGG 0.580862 5_4:-3371673 Msa0830340:CDS
CTGCGGCGGGAGAGAAGGTG+TGG 0.582701 5_4:-3371556 Msa0830340:CDS
AAACGGTGACGATCCGTCGA+TGG 0.583098 5_4:-3371750 Msa0830340:CDS
TTTCAGGATTCTTCTGACAC+CGG 0.588036 5_4:+3371771 None:intergenic
TTTGATCTGAAGGTTGAAGC+TGG 0.588078 5_4:-3371644 Msa0830340:CDS
GATACATCTGACTATGATGT+GGG 0.594052 5_4:-3370919 Msa0830340:three_prime_UTR
ATGAACAGACTGAGAATCGA+TGG 0.604081 5_4:-3371707 Msa0830340:CDS
TGGAATTTGAGAACAAGGAG+AGG 0.605032 5_4:-3371536 Msa0830340:CDS
TTCTCTTATGAGATATTGGG+GGG 0.610447 5_4:-3370782 Msa0830340:three_prime_UTR
TCTCAGACAACCACATACCA+GGG 0.619276 5_4:+3371166 None:intergenic
TGGTGGAGAAGGGTCGTCGA+CGG 0.620188 5_4:-3371579 Msa0830340:CDS
GAATCGATGGTGGTGATGAT+GGG 0.621168 5_4:-3371694 Msa0830340:CDS
TCGCCGTTAAGGAATGGTAA+CGG 0.633228 5_4:-3371455 Msa0830340:CDS
AAGAAGGAGAAAGAGAATGG+TGG 0.634822 5_4:-3371599 Msa0830340:CDS
GGCGGTGGTAAGTTAAGAGG+TGG 0.646062 5_4:-3371434 Msa0830340:CDS
CTGTTCATCCTATTCTCCGG+CGG 0.656983 5_4:+3371722 None:intergenic
TCCTAGCAGTCAGTTAACAC+AGG 0.658107 5_4:+3370957 None:intergenic
TCCGGCGGTGACACCATCGA+CGG 0.658472 5_4:+3371737 None:intergenic
TCTGGTGAAGAAGAAGAGAG+TGG 0.667630 5_4:-3371893 Msa0830340:CDS
CTTAACGGCGACAAACCGAG+AGG 0.678447 5_4:+3371467 None:intergenic
ATTCTCTTATGAGATATTGG+GGG 0.679935 5_4:-3370783 Msa0830340:three_prime_UTR
ATCTGAAGGTTGAAGCTGGT+AGG 0.709950 5_4:-3371640 Msa0830340:CDS
TGTTCCTGAGAATAATGGCA+AGG 0.728190 5_4:-3371112 Msa0830340:intron
TGTTAACTGACTGCTAGGAG+TGG 0.732009 5_4:-3370953 Msa0830340:three_prime_UTR
AACAGACTGAGAATCGATGG+TGG 0.737234 5_4:-3371704 Msa0830340:CDS
CCGTTAAGGAATGGTAACGG+CGG 0.752215 5_4:-3371452 Msa0830340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAATGAAATTAAAAATT+AGG + chr5_4:3371539-3371558 None:intergenic 10.0%
!! TCAAAATGAAATTAAAAATT+AGG + chr5_4:3371539-3371558 None:intergenic 10.0%
!! TATTCTTATAAACAAAAGAA+TGG + chr5_4:3371964-3371983 None:intergenic 15.0%
!! TATTCTTATAAACAAAAGAA+TGG + chr5_4:3371964-3371983 None:intergenic 15.0%
!! ATAATTCTCTTATGAGATAT+TGG - chr5_4:3371981-3372000 Msa0830340:five_prime_UTR 20.0%
!! TAATTCTCTTATGAGATATT+GGG - chr5_4:3371982-3372001 Msa0830340:five_prime_UTR 20.0%
!! ATAATTCTCTTATGAGATAT+TGG - chr5_4:3371981-3372000 Msa0830340:five_prime_UTR 20.0%
!! TAATTCTCTTATGAGATATT+GGG - chr5_4:3371982-3372001 Msa0830340:five_prime_UTR 20.0%
!!! TTTGTTGTTGTTTTGATCAA+TGG - chr5_4:3370846-3370865 Msa0830340:three_prime_UTR 25.0%
! GTTAAATTAAGTGTTCTGAT+TGG - chr5_4:3371731-3371750 Msa0830340:CDS 25.0%
! AATTCTCTTATGAGATATTG+GGG - chr5_4:3371983-3372002 Msa0830340:five_prime_UTR 25.0%
!!! TTTGTTGTTGTTTTGATCAA+TGG - chr5_4:3370846-3370865 Msa0830340:three_prime_UTR 25.0%
! GTTAAATTAAGTGTTCTGAT+TGG - chr5_4:3371731-3371750 Msa0830340:CDS 25.0%
! AATTCTCTTATGAGATATTG+GGG - chr5_4:3371983-3372002 Msa0830340:five_prime_UTR 25.0%
! ATTTTCTGAGCAAAATTGAG+AGG + chr5_4:3370759-3370778 None:intergenic 30.0%
! TTTTGATCAATGGTGTTTTC+TGG - chr5_4:3370856-3370875 Msa0830340:three_prime_UTR 30.0%
!! TCCATTTTCCTTATTTGAAG+TGG - chr5_4:3370956-3370975 Msa0830340:three_prime_UTR 30.0%
!! CCATTTTCCTTATTTGAAGT+GGG - chr5_4:3370957-3370976 Msa0830340:three_prime_UTR 30.0%
!! CATTTTCCTTATTTGAAGTG+GGG - chr5_4:3370958-3370977 Msa0830340:three_prime_UTR 30.0%
!! ATTTTCCTTATTTGAAGTGG+GGG - chr5_4:3370959-3370978 Msa0830340:three_prime_UTR 30.0%
ATTGATGAGAAAAAACCTCT+CGG - chr5_4:3371285-3371304 Msa0830340:intron 30.0%
!! ACATTTCTAGGTCTCTTTTT+GGG + chr5_4:3371459-3371478 None:intergenic 30.0%
!! AACATTTCTAGGTCTCTTTT+TGG + chr5_4:3371460-3371479 None:intergenic 30.0%
ATTTGCTTCTGAACATTTCT+AGG + chr5_4:3371471-3371490 None:intergenic 30.0%
TAGAAATGTTCAGAAGCAAA+TGG - chr5_4:3371470-3371489 Msa0830340:CDS 30.0%
!! GATTAATGCTGATTCCTATA+AGG - chr5_4:3371622-3371641 Msa0830340:CDS 30.0%
!!! TGTTCTGATTGGTTTGATTT+TGG - chr5_4:3371742-3371761 Msa0830340:CDS 30.0%
!!! TTTGATTTTGGTTTTTGCAG+AGG - chr5_4:3371754-3371773 Msa0830340:CDS 30.0%
CACAAATCAACTAAACTACT+GGG + chr5_4:3371896-3371915 None:intergenic 30.0%
ACACAAATCAACTAAACTAC+TGG + chr5_4:3371897-3371916 None:intergenic 30.0%
!!! GATGCTTTCAATGTTGATTT+TGG - chr5_4:3371922-3371941 Msa0830340:exon 30.0%
!!! AATGTTGATTTTGGTTCCAT+AGG - chr5_4:3371931-3371950 Msa0830340:five_prime_UTR 30.0%
!!! ATGTTGATTTTGGTTCCATA+GGG - chr5_4:3371932-3371951 Msa0830340:five_prime_UTR 30.0%
ATTCTCTTATGAGATATTGG+GGG - chr5_4:3371984-3372003 Msa0830340:five_prime_UTR 30.0%
! ATTTTCTGAGCAAAATTGAG+AGG + chr5_4:3370759-3370778 None:intergenic 30.0%
! TTTTGATCAATGGTGTTTTC+TGG - chr5_4:3370856-3370875 Msa0830340:three_prime_UTR 30.0%
!! TCCATTTTCCTTATTTGAAG+TGG - chr5_4:3370956-3370975 Msa0830340:three_prime_UTR 30.0%
!! CCATTTTCCTTATTTGAAGT+GGG - chr5_4:3370957-3370976 Msa0830340:three_prime_UTR 30.0%
!! CATTTTCCTTATTTGAAGTG+GGG - chr5_4:3370958-3370977 Msa0830340:three_prime_UTR 30.0%
!! ATTTTCCTTATTTGAAGTGG+GGG - chr5_4:3370959-3370978 Msa0830340:three_prime_UTR 30.0%
ATTGATGAGAAAAAACCTCT+CGG - chr5_4:3371285-3371304 Msa0830340:intron 30.0%
!! ACATTTCTAGGTCTCTTTTT+GGG + chr5_4:3371459-3371478 None:intergenic 30.0%
!! AACATTTCTAGGTCTCTTTT+TGG + chr5_4:3371460-3371479 None:intergenic 30.0%
ATTTGCTTCTGAACATTTCT+AGG + chr5_4:3371471-3371490 None:intergenic 30.0%
TAGAAATGTTCAGAAGCAAA+TGG - chr5_4:3371470-3371489 Msa0830340:CDS 30.0%
!! GATTAATGCTGATTCCTATA+AGG - chr5_4:3371622-3371641 Msa0830340:CDS 30.0%
!!! TGTTCTGATTGGTTTGATTT+TGG - chr5_4:3371742-3371761 Msa0830340:CDS 30.0%
!!! TTTGATTTTGGTTTTTGCAG+AGG - chr5_4:3371754-3371773 Msa0830340:CDS 30.0%
CACAAATCAACTAAACTACT+GGG + chr5_4:3371896-3371915 None:intergenic 30.0%
ACACAAATCAACTAAACTAC+TGG + chr5_4:3371897-3371916 None:intergenic 30.0%
!!! GATGCTTTCAATGTTGATTT+TGG - chr5_4:3371922-3371941 Msa0830340:exon 30.0%
!!! AATGTTGATTTTGGTTCCAT+AGG - chr5_4:3371931-3371950 Msa0830340:five_prime_UTR 30.0%
!!! ATGTTGATTTTGGTTCCATA+GGG - chr5_4:3371932-3371951 Msa0830340:five_prime_UTR 30.0%
ATTCTCTTATGAGATATTGG+GGG - chr5_4:3371984-3372003 Msa0830340:five_prime_UTR 30.0%
! AGAAACACTTTACGTTTTCC+CGG - chr5_4:3370701-3370720 Msa0830340:three_prime_UTR 35.0%
GAGAGGAAAGACAAAAACTT+GGG + chr5_4:3370742-3370761 None:intergenic 35.0%
TGAGAGGAAAGACAAAAACT+TGG + chr5_4:3370743-3370762 None:intergenic 35.0%
GCTCAGAAAATTCTTCAACT+CGG - chr5_4:3370768-3370787 Msa0830340:three_prime_UTR 35.0%
AACTCGGAGAAATCTCAATA+GGG - chr5_4:3370784-3370803 Msa0830340:three_prime_UTR 35.0%
GGAAAATGGAAGTTGTGAAA+AGG + chr5_4:3370946-3370965 None:intergenic 35.0%
CCCACTTCAAATAAGGAAAA+TGG + chr5_4:3370960-3370979 None:intergenic 35.0%
TGTCAGAAGAATCCTGAAAA+CGG - chr5_4:3371000-3371019 Msa0830340:intron 35.0%
!! AAGGTTGATGTTTGATCTGA+AGG - chr5_4:3371113-3371132 Msa0830340:CDS 35.0%
!! GAAAGATGCGATTTTGAAGA+AGG - chr5_4:3371152-3371171 Msa0830340:CDS 35.0%
TTGAAGAAGGAGAAAGAGAA+TGG - chr5_4:3371165-3371184 Msa0830340:CDS 35.0%
!! TTTTTCTGGAGAGAATGGAA+AGG - chr5_4:3371254-3371273 Msa0830340:intron 35.0%
!! TTTTCTGGAGAGAATGGAAA+GGG - chr5_4:3371255-3371274 Msa0830340:intron 35.0%
! TACTGTGTCATTGATGAAGA+AGG - chr5_4:3371383-3371402 Msa0830340:CDS 35.0%
AACATCATCAGGAACCTTAT+AGG + chr5_4:3371639-3371658 None:intergenic 35.0%
TTATTCTCAGGAACATCATC+AGG + chr5_4:3371650-3371669 None:intergenic 35.0%
GATGATGTTCCTGAGAATAA+TGG - chr5_4:3371650-3371669 Msa0830340:CDS 35.0%
AAGCAATGAGAATGACAACT+TGG - chr5_4:3371783-3371802 Msa0830340:CDS 35.0%
AATGAGAATGACAACTTGGA+AGG - chr5_4:3371787-3371806 Msa0830340:CDS 35.0%
GATACATCTGACTATGATGT+GGG - chr5_4:3371848-3371867 Msa0830340:CDS 35.0%
CTATGATGTGGGATTTGATT+TGG - chr5_4:3371859-3371878 Msa0830340:CDS 35.0%
!! AAAGAATGGACTATTCCCTA+TGG + chr5_4:3371950-3371969 None:intergenic 35.0%
TTCTCTTATGAGATATTGGG+GGG - chr5_4:3371985-3372004 Msa0830340:five_prime_UTR 35.0%
! AGAAACACTTTACGTTTTCC+CGG - chr5_4:3370701-3370720 Msa0830340:three_prime_UTR 35.0%
GAGAGGAAAGACAAAAACTT+GGG + chr5_4:3370742-3370761 None:intergenic 35.0%
TGAGAGGAAAGACAAAAACT+TGG + chr5_4:3370743-3370762 None:intergenic 35.0%
GCTCAGAAAATTCTTCAACT+CGG - chr5_4:3370768-3370787 Msa0830340:three_prime_UTR 35.0%
AACTCGGAGAAATCTCAATA+GGG - chr5_4:3370784-3370803 Msa0830340:three_prime_UTR 35.0%
GGAAAATGGAAGTTGTGAAA+AGG + chr5_4:3370946-3370965 None:intergenic 35.0%
CCCACTTCAAATAAGGAAAA+TGG + chr5_4:3370960-3370979 None:intergenic 35.0%
TGTCAGAAGAATCCTGAAAA+CGG - chr5_4:3371000-3371019 Msa0830340:intron 35.0%
!! AAGGTTGATGTTTGATCTGA+AGG - chr5_4:3371113-3371132 Msa0830340:CDS 35.0%
!! GAAAGATGCGATTTTGAAGA+AGG - chr5_4:3371152-3371171 Msa0830340:CDS 35.0%
TTGAAGAAGGAGAAAGAGAA+TGG - chr5_4:3371165-3371184 Msa0830340:CDS 35.0%
!! TTTTTCTGGAGAGAATGGAA+AGG - chr5_4:3371254-3371273 Msa0830340:intron 35.0%
!! TTTTCTGGAGAGAATGGAAA+GGG - chr5_4:3371255-3371274 Msa0830340:intron 35.0%
! TACTGTGTCATTGATGAAGA+AGG - chr5_4:3371383-3371402 Msa0830340:CDS 35.0%
AACATCATCAGGAACCTTAT+AGG + chr5_4:3371639-3371658 None:intergenic 35.0%
TTATTCTCAGGAACATCATC+AGG + chr5_4:3371650-3371669 None:intergenic 35.0%
GATGATGTTCCTGAGAATAA+TGG - chr5_4:3371650-3371669 Msa0830340:CDS 35.0%
AAGCAATGAGAATGACAACT+TGG - chr5_4:3371783-3371802 Msa0830340:CDS 35.0%
AATGAGAATGACAACTTGGA+AGG - chr5_4:3371787-3371806 Msa0830340:CDS 35.0%
GATACATCTGACTATGATGT+GGG - chr5_4:3371848-3371867 Msa0830340:CDS 35.0%
CTATGATGTGGGATTTGATT+TGG - chr5_4:3371859-3371878 Msa0830340:CDS 35.0%
!! AAAGAATGGACTATTCCCTA+TGG + chr5_4:3371950-3371969 None:intergenic 35.0%
TTCTCTTATGAGATATTGGG+GGG - chr5_4:3371985-3372004 Msa0830340:five_prime_UTR 35.0%
AGGAAAGACAAAAACTTGGG+TGG + chr5_4:3370739-3370758 None:intergenic 40.0%
CAACTCGGAGAAATCTCAAT+AGG - chr5_4:3370783-3370802 Msa0830340:three_prime_UTR 40.0%
CTTCAATCTTTCACCTTCAC+CGG + chr5_4:3370920-3370939 None:intergenic 40.0%
TGATTCCCCCACTTCAAATA+AGG + chr5_4:3370967-3370986 None:intergenic 40.0%
TTTCAGGATTCTTCTGACAC+CGG + chr5_4:3370999-3371018 None:intergenic 40.0%
AGTCTGTTCATCCTATTCTC+CGG + chr5_4:3371051-3371070 None:intergenic 40.0%
ATGAACAGACTGAGAATCGA+TGG - chr5_4:3371060-3371079 Msa0830340:intron 40.0%
TTTGATCTGAAGGTTGAAGC+TGG - chr5_4:3371123-3371142 Msa0830340:CDS 40.0%
AAGAAGGAGAAAGAGAATGG+TGG - chr5_4:3371168-3371187 Msa0830340:CDS 40.0%
AGGTGTGGAATTTGAGAACA+AGG - chr5_4:3371226-3371245 Msa0830340:intron 40.0%
TGGAATTTGAGAACAAGGAG+AGG - chr5_4:3371231-3371250 Msa0830340:intron 40.0%
! AGAACAAGGAGAGGTTTTTC+TGG - chr5_4:3371240-3371259 Msa0830340:intron 40.0%
!! AGAGGTTTTTCTGGAGAGAA+TGG - chr5_4:3371249-3371268 Msa0830340:intron 40.0%
GTAAACTTCACCTTCTTCTC+AGG + chr5_4:3371366-3371385 None:intergenic 40.0%
GAAGATTTCATCACGATGAC+TGG - chr5_4:3371414-3371433 Msa0830340:CDS 40.0%
TGTTCCTGAGAATAATGGCA+AGG - chr5_4:3371655-3371674 Msa0830340:CDS 40.0%
GGATACATCTGACTATGATG+TGG - chr5_4:3371847-3371866 Msa0830340:CDS 40.0%
AGGAAAGACAAAAACTTGGG+TGG + chr5_4:3370739-3370758 None:intergenic 40.0%
CAACTCGGAGAAATCTCAAT+AGG - chr5_4:3370783-3370802 Msa0830340:three_prime_UTR 40.0%
CTTCAATCTTTCACCTTCAC+CGG + chr5_4:3370920-3370939 None:intergenic 40.0%
TGATTCCCCCACTTCAAATA+AGG + chr5_4:3370967-3370986 None:intergenic 40.0%
TTTCAGGATTCTTCTGACAC+CGG + chr5_4:3370999-3371018 None:intergenic 40.0%
AGTCTGTTCATCCTATTCTC+CGG + chr5_4:3371051-3371070 None:intergenic 40.0%
ATGAACAGACTGAGAATCGA+TGG - chr5_4:3371060-3371079 Msa0830340:intron 40.0%
TTTGATCTGAAGGTTGAAGC+TGG - chr5_4:3371123-3371142 Msa0830340:CDS 40.0%
AAGAAGGAGAAAGAGAATGG+TGG - chr5_4:3371168-3371187 Msa0830340:CDS 40.0%
AGGTGTGGAATTTGAGAACA+AGG - chr5_4:3371226-3371245 Msa0830340:intron 40.0%
TGGAATTTGAGAACAAGGAG+AGG - chr5_4:3371231-3371250 Msa0830340:intron 40.0%
! AGAACAAGGAGAGGTTTTTC+TGG - chr5_4:3371240-3371259 Msa0830340:intron 40.0%
!! AGAGGTTTTTCTGGAGAGAA+TGG - chr5_4:3371249-3371268 Msa0830340:intron 40.0%
GTAAACTTCACCTTCTTCTC+AGG + chr5_4:3371366-3371385 None:intergenic 40.0%
GAAGATTTCATCACGATGAC+TGG - chr5_4:3371414-3371433 Msa0830340:CDS 40.0%
TGTTCCTGAGAATAATGGCA+AGG - chr5_4:3371655-3371674 Msa0830340:CDS 40.0%
GGATACATCTGACTATGATG+TGG - chr5_4:3371847-3371866 Msa0830340:CDS 40.0%
TCTGGTGAAGAAGAAGAGAG+TGG - chr5_4:3370874-3370893 Msa0830340:three_prime_UTR 45.0%
!! AGAAGAGAGTGGTGCTGTTA+TGG - chr5_4:3370885-3370904 Msa0830340:three_prime_UTR 45.0%
!! GAAGAGAGTGGTGCTGTTAT+GGG - chr5_4:3370886-3370905 Msa0830340:three_prime_UTR 45.0%
!! AGTGGTGCTGTTATGGGTAA+TGG - chr5_4:3370892-3370911 Msa0830340:three_prime_UTR 45.0%
! GCTGTTATGGGTAATGGTTC+CGG - chr5_4:3370898-3370917 Msa0830340:three_prime_UTR 45.0%
AACAGACTGAGAATCGATGG+TGG - chr5_4:3371063-3371082 Msa0830340:intron 45.0%
! AGAATCGATGGTGGTGATGA+TGG - chr5_4:3371072-3371091 Msa0830340:intron 45.0%
! GAATCGATGGTGGTGATGAT+GGG - chr5_4:3371073-3371092 Msa0830340:intron 45.0%
ATCTGAAGGTTGAAGCTGGT+AGG - chr5_4:3371127-3371146 Msa0830340:CDS 45.0%
AAGGAGAAAGAGAATGGTGG+TGG - chr5_4:3371171-3371190 Msa0830340:CDS 45.0%
!! AAAGAGAATGGTGGTGGAGA+AGG - chr5_4:3371177-3371196 Msa0830340:CDS 45.0%
!! AAGAGAATGGTGGTGGAGAA+GGG - chr5_4:3371178-3371197 Msa0830340:intron 45.0%
TCGCCGTTAAGGAATGGTAA+CGG - chr5_4:3371312-3371331 Msa0830340:CDS 45.0%
CTGGTCAAAAGCCTCATAGA+AGG - chr5_4:3371433-3371452 Msa0830340:CDS 45.0%
!! CTCTTTTTGGGCCTTCTATG+AGG + chr5_4:3371447-3371466 None:intergenic 45.0%
CAACCACATACCAGGGAAAA+GGG + chr5_4:3371597-3371616 None:intergenic 45.0%
ACAACCACATACCAGGGAAA+AGG + chr5_4:3371598-3371617 None:intergenic 45.0%
TCTCAGACAACCACATACCA+GGG + chr5_4:3371604-3371623 None:intergenic 45.0%
ATCTCAGACAACCACATACC+AGG + chr5_4:3371605-3371624 None:intergenic 45.0%
CACACCTTGCCATTATTCTC+AGG + chr5_4:3371662-3371681 None:intergenic 45.0%
TCCTAGCAGTCAGTTAACAC+AGG + chr5_4:3371813-3371832 None:intergenic 45.0%
TGTTAACTGACTGCTAGGAG+TGG - chr5_4:3371814-3371833 Msa0830340:CDS 45.0%
TCTTATGAGATATTGGGGGG+AGG - chr5_4:3371988-3372007 Msa0830340:five_prime_UTR 45.0%
CTTATGAGATATTGGGGGGA+GGG - chr5_4:3371989-3372008 Msa0830340:five_prime_UTR 45.0%
TCTGGTGAAGAAGAAGAGAG+TGG - chr5_4:3370874-3370893 Msa0830340:three_prime_UTR 45.0%
!! AGAAGAGAGTGGTGCTGTTA+TGG - chr5_4:3370885-3370904 Msa0830340:three_prime_UTR 45.0%
!! GAAGAGAGTGGTGCTGTTAT+GGG - chr5_4:3370886-3370905 Msa0830340:three_prime_UTR 45.0%
!! AGTGGTGCTGTTATGGGTAA+TGG - chr5_4:3370892-3370911 Msa0830340:three_prime_UTR 45.0%
! GCTGTTATGGGTAATGGTTC+CGG - chr5_4:3370898-3370917 Msa0830340:three_prime_UTR 45.0%
AACAGACTGAGAATCGATGG+TGG - chr5_4:3371063-3371082 Msa0830340:intron 45.0%
! AGAATCGATGGTGGTGATGA+TGG - chr5_4:3371072-3371091 Msa0830340:intron 45.0%
! GAATCGATGGTGGTGATGAT+GGG - chr5_4:3371073-3371092 Msa0830340:intron 45.0%
ATCTGAAGGTTGAAGCTGGT+AGG - chr5_4:3371127-3371146 Msa0830340:CDS 45.0%
AAGGAGAAAGAGAATGGTGG+TGG - chr5_4:3371171-3371190 Msa0830340:CDS 45.0%
!! AAAGAGAATGGTGGTGGAGA+AGG - chr5_4:3371177-3371196 Msa0830340:CDS 45.0%
!! AAGAGAATGGTGGTGGAGAA+GGG - chr5_4:3371178-3371197 Msa0830340:intron 45.0%
TCGCCGTTAAGGAATGGTAA+CGG - chr5_4:3371312-3371331 Msa0830340:CDS 45.0%
CTGGTCAAAAGCCTCATAGA+AGG - chr5_4:3371433-3371452 Msa0830340:CDS 45.0%
!! CTCTTTTTGGGCCTTCTATG+AGG + chr5_4:3371447-3371466 None:intergenic 45.0%
CAACCACATACCAGGGAAAA+GGG + chr5_4:3371597-3371616 None:intergenic 45.0%
ACAACCACATACCAGGGAAA+AGG + chr5_4:3371598-3371617 None:intergenic 45.0%
TCTCAGACAACCACATACCA+GGG + chr5_4:3371604-3371623 None:intergenic 45.0%
ATCTCAGACAACCACATACC+AGG + chr5_4:3371605-3371624 None:intergenic 45.0%
CACACCTTGCCATTATTCTC+AGG + chr5_4:3371662-3371681 None:intergenic 45.0%
TCCTAGCAGTCAGTTAACAC+AGG + chr5_4:3371813-3371832 None:intergenic 45.0%
TGTTAACTGACTGCTAGGAG+TGG - chr5_4:3371814-3371833 Msa0830340:CDS 45.0%
TCTTATGAGATATTGGGGGG+AGG - chr5_4:3371988-3372007 Msa0830340:five_prime_UTR 45.0%
CTTATGAGATATTGGGGGGA+GGG - chr5_4:3371989-3372008 Msa0830340:five_prime_UTR 45.0%
! ATGGGTAATGGTTCCGGTGA+AGG - chr5_4:3370904-3370923 Msa0830340:three_prime_UTR 50.0%
CTGTTCATCCTATTCTCCGG+CGG + chr5_4:3371048-3371067 None:intergenic 50.0%
GGATCGATGCTATGCGTGAA+AGG - chr5_4:3371094-3371113 Msa0830340:intron 50.0%
GGTTTGTCGCCGTTAAGGAA+TGG - chr5_4:3371306-3371325 Msa0830340:CDS 50.0%
CCGCCGTTACCATTCCTTAA+CGG + chr5_4:3371318-3371337 None:intergenic 50.0%
!! CCGTTAAGGAATGGTAACGG+CGG - chr5_4:3371315-3371334 Msa0830340:CDS 50.0%
!! TTAAGGAATGGTAACGGCGG+TGG - chr5_4:3371318-3371337 Msa0830340:CDS 50.0%
AACGGCGGTGGTAAGTTAAG+AGG - chr5_4:3371330-3371349 Msa0830340:CDS 50.0%
GGTGGTAAGTTAAGAGGTGG+TGG - chr5_4:3371336-3371355 Msa0830340:CDS 50.0%
TGGTGGTTGTCCTGAGAAGA+AGG - chr5_4:3371353-3371372 Msa0830340:CDS 50.0%
! TTTGTGCAGACCCTTTTCCC+TGG - chr5_4:3371584-3371603 Msa0830340:CDS 50.0%
! AGACCCTTTTCCCTGGTATG+TGG - chr5_4:3371591-3371610 Msa0830340:CDS 50.0%
GCCTGTGTTAACTGACTGCT+AGG - chr5_4:3371809-3371828 Msa0830340:CDS 50.0%
GCTAGGAGTGGTAACAGCTT+AGG - chr5_4:3371826-3371845 Msa0830340:CDS 50.0%
! ATGGGTAATGGTTCCGGTGA+AGG - chr5_4:3370904-3370923 Msa0830340:three_prime_UTR 50.0%
CTGTTCATCCTATTCTCCGG+CGG + chr5_4:3371048-3371067 None:intergenic 50.0%
GGATCGATGCTATGCGTGAA+AGG - chr5_4:3371094-3371113 Msa0830340:intron 50.0%
GGTTTGTCGCCGTTAAGGAA+TGG - chr5_4:3371306-3371325 Msa0830340:CDS 50.0%
CCGCCGTTACCATTCCTTAA+CGG + chr5_4:3371318-3371337 None:intergenic 50.0%
!! CCGTTAAGGAATGGTAACGG+CGG - chr5_4:3371315-3371334 Msa0830340:CDS 50.0%
!! TTAAGGAATGGTAACGGCGG+TGG - chr5_4:3371318-3371337 Msa0830340:CDS 50.0%
AACGGCGGTGGTAAGTTAAG+AGG - chr5_4:3371330-3371349 Msa0830340:CDS 50.0%
GGTGGTAAGTTAAGAGGTGG+TGG - chr5_4:3371336-3371355 Msa0830340:CDS 50.0%
TGGTGGTTGTCCTGAGAAGA+AGG - chr5_4:3371353-3371372 Msa0830340:CDS 50.0%
! TTTGTGCAGACCCTTTTCCC+TGG - chr5_4:3371584-3371603 Msa0830340:CDS 50.0%
! AGACCCTTTTCCCTGGTATG+TGG - chr5_4:3371591-3371610 Msa0830340:CDS 50.0%
GCCTGTGTTAACTGACTGCT+AGG - chr5_4:3371809-3371828 Msa0830340:CDS 50.0%
GCTAGGAGTGGTAACAGCTT+AGG - chr5_4:3371826-3371845 Msa0830340:CDS 50.0%
TGGGTGGCTTGAAAACTCGC+CGG + chr5_4:3370723-3370742 None:intergenic 55.0%
! GACGGATCGTCACCGTTTTC+AGG + chr5_4:3371015-3371034 None:intergenic 55.0%
AAACGGTGACGATCCGTCGA+TGG - chr5_4:3371017-3371036 Msa0830340:intron 55.0%
CTTAACGGCGACAAACCGAG+AGG + chr5_4:3371303-3371322 None:intergenic 55.0%
! CTCTCGGTTTGTCGCCGTTA+AGG - chr5_4:3371301-3371320 Msa0830340:CDS 55.0%
GGCGGTGGTAAGTTAAGAGG+TGG - chr5_4:3371333-3371352 Msa0830340:CDS 55.0%
TGGGTGGCTTGAAAACTCGC+CGG + chr5_4:3370723-3370742 None:intergenic 55.0%
! GACGGATCGTCACCGTTTTC+AGG + chr5_4:3371015-3371034 None:intergenic 55.0%
AAACGGTGACGATCCGTCGA+TGG - chr5_4:3371017-3371036 Msa0830340:intron 55.0%
CTTAACGGCGACAAACCGAG+AGG + chr5_4:3371303-3371322 None:intergenic 55.0%
! CTCTCGGTTTGTCGCCGTTA+AGG - chr5_4:3371301-3371320 Msa0830340:CDS 55.0%
GGCGGTGGTAAGTTAAGAGG+TGG - chr5_4:3371333-3371352 Msa0830340:CDS 55.0%
GGGTGGCTTGAAAACTCGCC+GGG + chr5_4:3370722-3370741 None:intergenic 60.0%
GGGGGAATCAGCGTTCTCTC+CGG - chr5_4:3370977-3370996 Msa0830340:three_prime_UTR 60.0%
!! TGGTGTCACCGCCGGAGAAT+AGG - chr5_4:3371037-3371056 Msa0830340:intron 60.0%
TGGTGGAGAAGGGTCGTCGA+CGG - chr5_4:3371188-3371207 Msa0830340:intron 60.0%
GGGTGGCTTGAAAACTCGCC+GGG + chr5_4:3370722-3370741 None:intergenic 60.0%
GGGGGAATCAGCGTTCTCTC+CGG - chr5_4:3370977-3370996 Msa0830340:three_prime_UTR 60.0%
!! TGGTGTCACCGCCGGAGAAT+AGG - chr5_4:3371037-3371056 Msa0830340:intron 60.0%
TGGTGGAGAAGGGTCGTCGA+CGG - chr5_4:3371188-3371207 Msa0830340:intron 60.0%
!! TCCGTCGATGGTGTCACCGC+CGG - chr5_4:3371029-3371048 Msa0830340:intron 65.0%
TCCGGCGGTGACACCATCGA+CGG + chr5_4:3371033-3371052 None:intergenic 65.0%
AGAAGGGTCGTCGACGGCTG+CGG - chr5_4:3371194-3371213 Msa0830340:intron 65.0%
!! TCCGTCGATGGTGTCACCGC+CGG - chr5_4:3371029-3371048 Msa0830340:intron 65.0%
TCCGGCGGTGACACCATCGA+CGG + chr5_4:3371033-3371052 None:intergenic 65.0%
AGAAGGGTCGTCGACGGCTG+CGG - chr5_4:3371194-3371213 Msa0830340:intron 65.0%
CTGCGGCGGGAGAGAAGGTG+TGG - chr5_4:3371211-3371230 Msa0830340:intron 70.0%
CTGCGGCGGGAGAGAAGGTG+TGG - chr5_4:3371211-3371230 Msa0830340:intron 70.0%
AGGGTCGTCGACGGCTGCGG+CGG - chr5_4:3371197-3371216 Msa0830340:intron 75.0%
GACGGCTGCGGCGGGAGAGA+AGG - chr5_4:3371206-3371225 Msa0830340:intron 75.0%
AGGGTCGTCGACGGCTGCGG+CGG - chr5_4:3371197-3371216 Msa0830340:intron 75.0%
GACGGCTGCGGCGGGAGAGA+AGG - chr5_4:3371206-3371225 Msa0830340:intron 75.0%
! GGGTCGTCGACGGCTGCGGC+GGG - chr5_4:3371198-3371217 Msa0830340:intron 80.0%
! GGGTCGTCGACGGCTGCGGC+GGG - chr5_4:3371198-3371217 Msa0830340:intron 80.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 3370699 3372090 3370699 ID=Msa0830340;Name=Msa0830340
chr5_4 mRNA 3370699 3372090 3370699 ID=Msa0830340-mRNA-1;Parent=Msa0830340;Name=Msa0830340-mRNA-1;_AED=0.04;_eAED=0.04;_QI=166|1|1|1|1|1|3|311|239
chr5_4 exon 3370699 3371015 3370699 ID=Msa0830340-mRNA-1:exon:1250;Parent=Msa0830340-mRNA-1
chr5_4 exon 3371113 3371196 3371113 ID=Msa0830340-mRNA-1:exon:1249;Parent=Msa0830340-mRNA-1
chr5_4 exon 3371295 3372090 3371295 ID=Msa0830340-mRNA-1:exon:1248;Parent=Msa0830340-mRNA-1
chr5_4 five_prime_UTR 3371925 3372090 3371925 ID=Msa0830340-mRNA-1:five_prime_utr;Parent=Msa0830340-mRNA-1
chr5_4 CDS 3371295 3371924 3371295 ID=Msa0830340-mRNA-1:cds;Parent=Msa0830340-mRNA-1
chr5_4 CDS 3371113 3371196 3371113 ID=Msa0830340-mRNA-1:cds;Parent=Msa0830340-mRNA-1
chr5_4 CDS 3371010 3371015 3371010 ID=Msa0830340-mRNA-1:cds;Parent=Msa0830340-mRNA-1
chr5_4 three_prime_UTR 3370699 3371009 3370699 ID=Msa0830340-mRNA-1:three_prime_utr;Parent=Msa0830340-mRNA-1
Gene Sequence

>Msa0830340

ATGGTGTTTTCTGGTGAAGAAGAAGAGAGTGGTGCTGTTATGGGTAATGGTTCCGGTGAAGGTGAAAGATTGAAGCATCCTTTTCACAACTTCCATTTTCCTTATTTGAAGTGGGGGAATCAGCGTTCTCTCCGGTGTCAGAAGAATCCTGAAAACGGTGACGATCCGTCGATGGTGTCACCGCCGGAGAATAGGATGAACAGACTGAGAATCGATGGTGGTGATGATGGGATCGATGCTATGCGTGAAAGGTTGATGTTTGATCTGAAGGTTGAAGCTGGTAGGATGAAAGATGCGATTTTGAAGAAGGAGAAAGAGAATGGTGGTGGAGAAGGGTCGTCGACGGCTGCGGCGGGAGAGAAGGTGTGGAATTTGAGAACAAGGAGAGGTTTTTCTGGAGAGAATGGAAAGGGATTGAAGATTGATGAGAAAAAACCTCTCGGTTTGTCGCCGTTAAGGAATGGTAACGGCGGTGGTAAGTTAAGAGGTGGTGGTTGTCCTGAGAAGAAGGTGAAGTTTACTGTGTCATTGATGAAGAAGGAGATTGAAGAAGATTTCATCACGATGACTGGTCAAAAGCCTCATAGAAGGCCCAAAAAGAGACCTAGAAATGTTCAGAAGCAAATGGATACCCTTTTCCCTGGTATGTGGTTGTCTGAGATTAATGCTGATTCCTATAAGGTTCCTGATGATGTTCCTGAGAATAATGGCAAGAGGTAG
Protein sequence

>Msa0830340

MVFSGEEEESGAVMGNGSGEGERLKHPFHNFHFPYLKWGNQRSLRCQKNPENGDDPSMVSPPENRMNRLRIDGGDDGIDAMRERLMFDLKVEAGRMKDAILKKEKENGGGEGSSTAAAGEKVWNLRTRRGFSGENGKGLKIDEKKPLGLSPLRNGNGGGKLRGGGCPEKKVKFTVSLMKKEIEEDFITMTGQKPHRRPKKRPRNVQKQMDTLFPGMWLSEINADSYKVPDDVPENNGKR*