AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1429640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1429640 MtrunA17_Chr1g0209591 92.887 239 17 0 1 239 1 239 1.16e-163 452
Msa1429640 MtrunA17_Chr6g0451821 60.630 254 73 9 1 238 1 243 1.73e-87 260
Msa1429640 MtrunA17_Chr5g0438001 64.173 254 65 9 1 238 1 244 3.78e-85 254
Msa1429640 MtrunA17_Chr1g0209581 59.389 229 66 8 1 211 1 220 2.25e-71 217
Msa1429640 MtrunA17_Chr6g0484191 36.364 253 99 9 20 234 2 230 3.16e-36 127
Msa1429640 MtrunA17_Chr5g0423311 35.169 236 121 7 25 238 25 250 9.54e-32 117
Msa1429640 MtrunA17_Chr4g0055861 32.653 245 108 8 26 230 11 238 7.07e-26 101
Msa1429640 MtrunA17_Chr5g0423331 35.714 168 67 7 77 230 73 213 1.38e-21 89.7
Msa1429640 MtrunA17_Chr5g0423281 33.333 213 105 10 28 230 17 202 2.47e-21 88.6
Msa1429640 MtrunA17_Chr8g0335091 35.484 124 58 3 119 236 445 552 1.78e-12 66.2
Msa1429640 MtrunA17_Chr8g0369051 45.614 57 31 0 172 228 171 227 2.06e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1429640 AT1G25370.2 34.532 278 119 10 1 230 1 263 2.20e-31 116
Msa1429640 AT1G25370.1 34.532 278 119 10 1 230 1 263 2.20e-31 116
Msa1429640 AT1G68340.2 32.000 250 122 8 17 230 2 239 5.72e-27 104
Msa1429640 AT1G68340.1 32.000 250 122 8 17 230 2 239 5.85e-27 104
Msa1429640 AT3G18295.1 41.014 217 101 8 28 232 13 214 7.16e-27 103
Msa1429640 AT3G60410.1 43.200 125 56 4 119 228 196 320 7.26e-21 89.7
Msa1429640 AT3G60410.2 43.200 125 56 4 119 228 196 320 7.26e-21 89.7
Msa1429640 AT3G60410.3 43.200 125 56 4 119 228 196 320 7.26e-21 89.7
Msa1429640 AT1G68340.3 31.169 231 111 8 17 211 2 220 1.05e-20 87.8
Msa1429640 AT1G48770.1 33.831 201 96 6 28 226 11 176 3.27e-15 71.6
Msa1429640 AT1G48770.2 33.831 201 96 6 28 226 11 176 3.27e-15 71.6
Msa1429640 AT1G48770.3 33.831 201 96 6 28 226 11 176 3.27e-15 71.6
Msa1429640 AT4G17440.1 33.071 127 64 3 123 228 83 209 4.74e-13 66.6
Msa1429640 AT4G17440.2 33.071 127 64 3 123 228 83 209 4.74e-13 66.6
Msa1429640 AT4G17440.3 33.071 127 64 3 123 228 83 209 5.79e-13 66.6
Msa1429640 AT4G17440.4 32.061 131 64 4 123 228 83 213 5.67e-11 60.8

Find 89 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCTTTCAATGTTGATTT+TGG 0.185271 tig0026539:+21348 Msa1429640:three_prime_UTR
ATTTGCTTCTGAACATTTCT+AGG 0.189823 tig0026539:-20894 None:intergenic
AGAAGAGAGTGGTGCTGTTA+TGG 0.254438 tig0026539:+20311 Msa1429640:CDS
CCCACTTCAAATAAGGAAAA+TGG 0.259092 tig0026539:-20383 None:intergenic
ATAATTCTCTTATGAGATAT+TGG 0.275131 tig0026539:+21407 Msa1429640:three_prime_UTR
TAATTCTCTTATGAGATATT+GGG 0.311967 tig0026539:+21408 Msa1429640:three_prime_UTR
CTCTCGGTTTGTCGCCGTTA+AGG 0.320855 tig0026539:+20727 Msa1429640:CDS
CTATGATGTGGGATTTGATT+TGG 0.324597 tig0026539:+21285 Msa1429640:three_prime_UTR
GAAGAGAGTGGTGCTGTTAT+GGG 0.326106 tig0026539:+20312 Msa1429640:CDS
AACATCATCAGGAACCTTAT+AGG 0.333378 tig0026539:-21062 None:intergenic
CAACTCGGAGAAATCTCAAT+AGG 0.347215 tig0026539:+20209 Msa1429640:five_prime_UTR
GATTAATGCTGATTCCTATA+AGG 0.358878 tig0026539:+21048 Msa1429640:CDS
AAAGAGAATGGTGGTGGAGA+AGG 0.384258 tig0026539:+20603 Msa1429640:CDS
CTTCAATCTTTCACCTTCAC+CGG 0.392665 tig0026539:-20343 None:intergenic
ACACAAATCAACTAAACTAC+TGG 0.402438 tig0026539:-21320 None:intergenic
GTAAACTTCACCTTCTTCTC+AGG 0.423971 tig0026539:-20789 None:intergenic
CCGCCGTTACCATTCCTTAA+CGG 0.425360 tig0026539:-20741 None:intergenic
TATTCTTATAAACAAAAGAA+TGG 0.433263 tig0026539:-21387 None:intergenic
GATGATGTTCCTGAGAATAA+TGG 0.435032 tig0026539:+21076 Msa1429640:CDS
AACTCGGAGAAATCTCAATA+GGG 0.438602 tig0026539:+20210 Msa1429640:five_prime_UTR
AAGAGAATGGTGGTGGAGAA+GGG 0.452542 tig0026539:+20604 Msa1429640:CDS
TTGAAGAAGGAGAAAGAGAA+TGG 0.453989 tig0026539:+20591 Msa1429640:CDS
TGGTGTCACCGCCGGAGAAT+AGG 0.459611 tig0026539:+20463 Msa1429640:CDS
GGGTGGCTTGAAAACTCGCC+GGG 0.466575 tig0026539:-20145 None:intergenic
GCTGTTATGGGTAATGGTTC+CGG 0.467366 tig0026539:+20324 Msa1429640:CDS
TTATTCTCAGGAACATCATC+AGG 0.474050 tig0026539:-21073 None:intergenic
CACACCTTGCCATTATTCTC+AGG 0.477305 tig0026539:-21085 None:intergenic
AAGGAGAAAGAGAATGGTGG+TGG 0.478905 tig0026539:+20597 Msa1429640:CDS
GAAGATTTCATCACGATGAC+TGG 0.482101 tig0026539:+20840 Msa1429640:CDS
AGAATCGATGGTGGTGATGA+TGG 0.483584 tig0026539:+20498 Msa1429640:CDS
ACAACCACATACCAGGGAAA+AGG 0.488217 tig0026539:-21021 None:intergenic
TACTGTGTCATTGATGAAGA+AGG 0.488455 tig0026539:+20809 Msa1429640:CDS
TAGAAATGTTCAGAAGCAAA+TGG 0.488562 tig0026539:+20896 Msa1429640:CDS
CAACCACATACCAGGGAAAA+GGG 0.489538 tig0026539:-21020 None:intergenic
GGGTCGTCGACGGCTGCGGC+GGG 0.492188 tig0026539:+20624 Msa1429640:CDS
GGAAAATGGAAGTTGTGAAA+AGG 0.494064 tig0026539:-20369 None:intergenic
AGAAGGGTCGTCGACGGCTG+CGG 0.494461 tig0026539:+20620 Msa1429640:CDS
GCTAGGAGTGGTAACAGCTT+AGG 0.503173 tig0026539:+21252 Msa1429640:three_prime_UTR
GACGGCTGCGGCGGGAGAGA+AGG 0.504619 tig0026539:+20632 Msa1429640:CDS
ATCTCAGACAACCACATACC+AGG 0.508509 tig0026539:-21028 None:intergenic
AAGGTTGATGTTTGATCTGA+AGG 0.512312 tig0026539:+20539 Msa1429640:CDS
AGGGTCGTCGACGGCTGCGG+CGG 0.517125 tig0026539:+20623 Msa1429640:CDS
AACGGCGGTGGTAAGTTAAG+AGG 0.518465 tig0026539:+20756 Msa1429640:CDS
AGTGGTGCTGTTATGGGTAA+TGG 0.525672 tig0026539:+20318 Msa1429640:CDS
AGGTGTGGAATTTGAGAACA+AGG 0.527843 tig0026539:+20652 Msa1429640:CDS
GGTTTGTCGCCGTTAAGGAA+TGG 0.536117 tig0026539:+20732 Msa1429640:CDS
AATTCTCTTATGAGATATTG+GGG 0.539956 tig0026539:+21409 Msa1429640:three_prime_UTR
AAAGAATGGACTATTCCCTA+TGG 0.543621 tig0026539:-21373 None:intergenic
AAGCAATGAGAATGACAACT+TGG 0.552322 tig0026539:+21209 Msa1429640:three_prime_UTR
TGTCAGAAGAATCCTGAAAA+CGG 0.554244 tig0026539:+20426 Msa1429640:CDS
GCCTGTGTTAACTGACTGCT+AGG 0.556311 tig0026539:+21235 Msa1429640:three_prime_UTR
CTGGTCAAAAGCCTCATAGA+AGG 0.556564 tig0026539:+20859 Msa1429640:CDS
GCTCAGAAAATTCTTCAACT+CGG 0.562918 tig0026539:+20194 Msa1429640:five_prime_UTR
ATGGGTAATGGTTCCGGTGA+AGG 0.563977 tig0026539:+20330 Msa1429640:CDS
TGGTGGTTGTCCTGAGAAGA+AGG 0.564497 tig0026539:+20779 Msa1429640:CDS
AGTCTGTTCATCCTATTCTC+CGG 0.565451 tig0026539:-20474 None:intergenic
CACAAATCAACTAAACTACT+GGG 0.566998 tig0026539:-21319 None:intergenic
TGGGTGGCTTGAAAACTCGC+CGG 0.570214 tig0026539:-20146 None:intergenic
TTAAGGAATGGTAACGGCGG+TGG 0.572770 tig0026539:+20744 Msa1429640:CDS
GGATACATCTGACTATGATG+TGG 0.574402 tig0026539:+21273 Msa1429640:three_prime_UTR
TCCGTCGATGGTGTCACCGC+CGG 0.576373 tig0026539:+20455 Msa1429640:CDS
AATGAGAATGACAACTTGGA+AGG 0.579939 tig0026539:+21213 Msa1429640:three_prime_UTR
GGTGGTAAGTTAAGAGGTGG+TGG 0.580505 tig0026539:+20762 Msa1429640:CDS
GGATCGATGCTATGCGTGAA+AGG 0.580862 tig0026539:+20520 Msa1429640:CDS
CTGCGGCGGGAGAGAAGGTG+TGG 0.582701 tig0026539:+20637 Msa1429640:CDS
AAACGGTGACGATCCGTCGA+TGG 0.583098 tig0026539:+20443 Msa1429640:CDS
TTTCAGGATTCTTCTGACAC+CGG 0.588036 tig0026539:-20422 None:intergenic
TTTGATCTGAAGGTTGAAGC+TGG 0.588078 tig0026539:+20549 Msa1429640:CDS
GATACATCTGACTATGATGT+GGG 0.594052 tig0026539:+21274 Msa1429640:three_prime_UTR
ATGAACAGACTGAGAATCGA+TGG 0.604081 tig0026539:+20486 Msa1429640:CDS
TGGAATTTGAGAACAAGGAG+AGG 0.605032 tig0026539:+20657 Msa1429640:CDS
TTCTCTTATGAGATATTGGG+GGG 0.610447 tig0026539:+21411 Msa1429640:three_prime_UTR
TCTCAGACAACCACATACCA+GGG 0.619276 tig0026539:-21027 None:intergenic
TGGTGGAGAAGGGTCGTCGA+CGG 0.620188 tig0026539:+20614 Msa1429640:CDS
GAATCGATGGTGGTGATGAT+GGG 0.621168 tig0026539:+20499 Msa1429640:CDS
TCGCCGTTAAGGAATGGTAA+CGG 0.633228 tig0026539:+20738 Msa1429640:CDS
AAGAAGGAGAAAGAGAATGG+TGG 0.634822 tig0026539:+20594 Msa1429640:CDS
GGCGGTGGTAAGTTAAGAGG+TGG 0.646062 tig0026539:+20759 Msa1429640:CDS
CTGTTCATCCTATTCTCCGG+CGG 0.656983 tig0026539:-20471 None:intergenic
TCCTAGCAGTCAGTTAACAC+AGG 0.658107 tig0026539:-21236 None:intergenic
TCCGGCGGTGACACCATCGA+CGG 0.658472 tig0026539:-20456 None:intergenic
TCTGGTGAAGAAGAAGAGAG+TGG 0.667630 tig0026539:+20300 Msa1429640:CDS
CTTAACGGCGACAAACCGAG+AGG 0.678447 tig0026539:-20726 None:intergenic
ATTCTCTTATGAGATATTGG+GGG 0.679935 tig0026539:+21410 Msa1429640:three_prime_UTR
ATCTGAAGGTTGAAGCTGGT+AGG 0.709950 tig0026539:+20553 Msa1429640:CDS
TGTTCCTGAGAATAATGGCA+AGG 0.728190 tig0026539:+21081 Msa1429640:CDS
TGTTAACTGACTGCTAGGAG+TGG 0.732009 tig0026539:+21240 Msa1429640:three_prime_UTR
AACAGACTGAGAATCGATGG+TGG 0.737234 tig0026539:+20489 Msa1429640:CDS
CCGTTAAGGAATGGTAACGG+CGG 0.752215 tig0026539:+20741 Msa1429640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAATGAAATTAAAAATT+AGG - tig0026539:20965-20984 None:intergenic 10.0%
!! TCAAAATGAAATTAAAAATT+AGG - tig0026539:20965-20984 None:intergenic 10.0%
!! TATTCTTATAAACAAAAGAA+TGG - tig0026539:21390-21409 None:intergenic 15.0%
!! TATTCTTATAAACAAAAGAA+TGG - tig0026539:21390-21409 None:intergenic 15.0%
!! ATAATTCTCTTATGAGATAT+TGG + tig0026539:21407-21426 Msa1429640:three_prime_UTR 20.0%
!! TAATTCTCTTATGAGATATT+GGG + tig0026539:21408-21427 Msa1429640:three_prime_UTR 20.0%
!! ATAATTCTCTTATGAGATAT+TGG + tig0026539:21407-21426 Msa1429640:three_prime_UTR 20.0%
!! TAATTCTCTTATGAGATATT+GGG + tig0026539:21408-21427 Msa1429640:three_prime_UTR 20.0%
!!! TTTGTTGTTGTTTTGATCAA+TGG + tig0026539:20272-20291 Msa1429640:exon 25.0%
! GTTAAATTAAGTGTTCTGAT+TGG + tig0026539:21157-21176 Msa1429640:intron 25.0%
! AATTCTCTTATGAGATATTG+GGG + tig0026539:21409-21428 Msa1429640:three_prime_UTR 25.0%
!!! TTTGTTGTTGTTTTGATCAA+TGG + tig0026539:20272-20291 Msa1429640:exon 25.0%
! GTTAAATTAAGTGTTCTGAT+TGG + tig0026539:21157-21176 Msa1429640:intron 25.0%
! AATTCTCTTATGAGATATTG+GGG + tig0026539:21409-21428 Msa1429640:three_prime_UTR 25.0%
! ATTTTCTGAGCAAAATTGAG+AGG - tig0026539:20185-20204 None:intergenic 30.0%
! TTTTGATCAATGGTGTTTTC+TGG + tig0026539:20282-20301 Msa1429640:exon 30.0%
!! TCCATTTTCCTTATTTGAAG+TGG + tig0026539:20382-20401 Msa1429640:CDS 30.0%
!! CCATTTTCCTTATTTGAAGT+GGG + tig0026539:20383-20402 Msa1429640:CDS 30.0%
!! CATTTTCCTTATTTGAAGTG+GGG + tig0026539:20384-20403 Msa1429640:CDS 30.0%
!! ATTTTCCTTATTTGAAGTGG+GGG + tig0026539:20385-20404 Msa1429640:CDS 30.0%
ATTGATGAGAAAAAACCTCT+CGG + tig0026539:20711-20730 Msa1429640:CDS 30.0%
!! ACATTTCTAGGTCTCTTTTT+GGG - tig0026539:20885-20904 None:intergenic 30.0%
!! AACATTTCTAGGTCTCTTTT+TGG - tig0026539:20886-20905 None:intergenic 30.0%
ATTTGCTTCTGAACATTTCT+AGG - tig0026539:20897-20916 None:intergenic 30.0%
TAGAAATGTTCAGAAGCAAA+TGG + tig0026539:20896-20915 Msa1429640:CDS 30.0%
!! GATTAATGCTGATTCCTATA+AGG + tig0026539:21048-21067 Msa1429640:CDS 30.0%
!!! TGTTCTGATTGGTTTGATTT+TGG + tig0026539:21168-21187 Msa1429640:intron 30.0%
!!! TTTGATTTTGGTTTTTGCAG+AGG + tig0026539:21180-21199 Msa1429640:intron 30.0%
CACAAATCAACTAAACTACT+GGG - tig0026539:21322-21341 None:intergenic 30.0%
ACACAAATCAACTAAACTAC+TGG - tig0026539:21323-21342 None:intergenic 30.0%
!!! GATGCTTTCAATGTTGATTT+TGG + tig0026539:21348-21367 Msa1429640:three_prime_UTR 30.0%
!!! AATGTTGATTTTGGTTCCAT+AGG + tig0026539:21357-21376 Msa1429640:three_prime_UTR 30.0%
!!! ATGTTGATTTTGGTTCCATA+GGG + tig0026539:21358-21377 Msa1429640:three_prime_UTR 30.0%
ATTCTCTTATGAGATATTGG+GGG + tig0026539:21410-21429 Msa1429640:three_prime_UTR 30.0%
! ATTTTCTGAGCAAAATTGAG+AGG - tig0026539:20185-20204 None:intergenic 30.0%
! TTTTGATCAATGGTGTTTTC+TGG + tig0026539:20282-20301 Msa1429640:exon 30.0%
!! TCCATTTTCCTTATTTGAAG+TGG + tig0026539:20382-20401 Msa1429640:CDS 30.0%
!! CCATTTTCCTTATTTGAAGT+GGG + tig0026539:20383-20402 Msa1429640:CDS 30.0%
!! CATTTTCCTTATTTGAAGTG+GGG + tig0026539:20384-20403 Msa1429640:CDS 30.0%
!! ATTTTCCTTATTTGAAGTGG+GGG + tig0026539:20385-20404 Msa1429640:CDS 30.0%
ATTGATGAGAAAAAACCTCT+CGG + tig0026539:20711-20730 Msa1429640:CDS 30.0%
!! ACATTTCTAGGTCTCTTTTT+GGG - tig0026539:20885-20904 None:intergenic 30.0%
!! AACATTTCTAGGTCTCTTTT+TGG - tig0026539:20886-20905 None:intergenic 30.0%
ATTTGCTTCTGAACATTTCT+AGG - tig0026539:20897-20916 None:intergenic 30.0%
TAGAAATGTTCAGAAGCAAA+TGG + tig0026539:20896-20915 Msa1429640:CDS 30.0%
!! GATTAATGCTGATTCCTATA+AGG + tig0026539:21048-21067 Msa1429640:CDS 30.0%
!!! TGTTCTGATTGGTTTGATTT+TGG + tig0026539:21168-21187 Msa1429640:intron 30.0%
!!! TTTGATTTTGGTTTTTGCAG+AGG + tig0026539:21180-21199 Msa1429640:intron 30.0%
CACAAATCAACTAAACTACT+GGG - tig0026539:21322-21341 None:intergenic 30.0%
ACACAAATCAACTAAACTAC+TGG - tig0026539:21323-21342 None:intergenic 30.0%
!!! GATGCTTTCAATGTTGATTT+TGG + tig0026539:21348-21367 Msa1429640:three_prime_UTR 30.0%
!!! AATGTTGATTTTGGTTCCAT+AGG + tig0026539:21357-21376 Msa1429640:three_prime_UTR 30.0%
!!! ATGTTGATTTTGGTTCCATA+GGG + tig0026539:21358-21377 Msa1429640:three_prime_UTR 30.0%
ATTCTCTTATGAGATATTGG+GGG + tig0026539:21410-21429 Msa1429640:three_prime_UTR 30.0%
! AGAAACACTTTACGTTTTCC+CGG + tig0026539:20127-20146 Msa1429640:five_prime_UTR 35.0%
GAGAGGAAAGACAAAAACTT+GGG - tig0026539:20168-20187 None:intergenic 35.0%
TGAGAGGAAAGACAAAAACT+TGG - tig0026539:20169-20188 None:intergenic 35.0%
GCTCAGAAAATTCTTCAACT+CGG + tig0026539:20194-20213 Msa1429640:five_prime_UTR 35.0%
AACTCGGAGAAATCTCAATA+GGG + tig0026539:20210-20229 Msa1429640:five_prime_UTR 35.0%
GGAAAATGGAAGTTGTGAAA+AGG - tig0026539:20372-20391 None:intergenic 35.0%
CCCACTTCAAATAAGGAAAA+TGG - tig0026539:20386-20405 None:intergenic 35.0%
TGTCAGAAGAATCCTGAAAA+CGG + tig0026539:20426-20445 Msa1429640:CDS 35.0%
!! AAGGTTGATGTTTGATCTGA+AGG + tig0026539:20539-20558 Msa1429640:CDS 35.0%
!! GAAAGATGCGATTTTGAAGA+AGG + tig0026539:20578-20597 Msa1429640:CDS 35.0%
TTGAAGAAGGAGAAAGAGAA+TGG + tig0026539:20591-20610 Msa1429640:CDS 35.0%
!! TTTTTCTGGAGAGAATGGAA+AGG + tig0026539:20680-20699 Msa1429640:CDS 35.0%
!! TTTTCTGGAGAGAATGGAAA+GGG + tig0026539:20681-20700 Msa1429640:CDS 35.0%
! TACTGTGTCATTGATGAAGA+AGG + tig0026539:20809-20828 Msa1429640:CDS 35.0%
AACATCATCAGGAACCTTAT+AGG - tig0026539:21065-21084 None:intergenic 35.0%
TTATTCTCAGGAACATCATC+AGG - tig0026539:21076-21095 None:intergenic 35.0%
GATGATGTTCCTGAGAATAA+TGG + tig0026539:21076-21095 Msa1429640:CDS 35.0%
AAGCAATGAGAATGACAACT+TGG + tig0026539:21209-21228 Msa1429640:three_prime_UTR 35.0%
AATGAGAATGACAACTTGGA+AGG + tig0026539:21213-21232 Msa1429640:three_prime_UTR 35.0%
GATACATCTGACTATGATGT+GGG + tig0026539:21274-21293 Msa1429640:three_prime_UTR 35.0%
CTATGATGTGGGATTTGATT+TGG + tig0026539:21285-21304 Msa1429640:three_prime_UTR 35.0%
!! AAAGAATGGACTATTCCCTA+TGG - tig0026539:21376-21395 None:intergenic 35.0%
TTCTCTTATGAGATATTGGG+GGG + tig0026539:21411-21430 Msa1429640:three_prime_UTR 35.0%
! AGAAACACTTTACGTTTTCC+CGG + tig0026539:20127-20146 Msa1429640:five_prime_UTR 35.0%
GAGAGGAAAGACAAAAACTT+GGG - tig0026539:20168-20187 None:intergenic 35.0%
TGAGAGGAAAGACAAAAACT+TGG - tig0026539:20169-20188 None:intergenic 35.0%
GCTCAGAAAATTCTTCAACT+CGG + tig0026539:20194-20213 Msa1429640:five_prime_UTR 35.0%
AACTCGGAGAAATCTCAATA+GGG + tig0026539:20210-20229 Msa1429640:five_prime_UTR 35.0%
GGAAAATGGAAGTTGTGAAA+AGG - tig0026539:20372-20391 None:intergenic 35.0%
CCCACTTCAAATAAGGAAAA+TGG - tig0026539:20386-20405 None:intergenic 35.0%
TGTCAGAAGAATCCTGAAAA+CGG + tig0026539:20426-20445 Msa1429640:CDS 35.0%
!! AAGGTTGATGTTTGATCTGA+AGG + tig0026539:20539-20558 Msa1429640:CDS 35.0%
!! GAAAGATGCGATTTTGAAGA+AGG + tig0026539:20578-20597 Msa1429640:CDS 35.0%
TTGAAGAAGGAGAAAGAGAA+TGG + tig0026539:20591-20610 Msa1429640:CDS 35.0%
!! TTTTTCTGGAGAGAATGGAA+AGG + tig0026539:20680-20699 Msa1429640:CDS 35.0%
!! TTTTCTGGAGAGAATGGAAA+GGG + tig0026539:20681-20700 Msa1429640:CDS 35.0%
! TACTGTGTCATTGATGAAGA+AGG + tig0026539:20809-20828 Msa1429640:CDS 35.0%
AACATCATCAGGAACCTTAT+AGG - tig0026539:21065-21084 None:intergenic 35.0%
TTATTCTCAGGAACATCATC+AGG - tig0026539:21076-21095 None:intergenic 35.0%
GATGATGTTCCTGAGAATAA+TGG + tig0026539:21076-21095 Msa1429640:CDS 35.0%
AAGCAATGAGAATGACAACT+TGG + tig0026539:21209-21228 Msa1429640:three_prime_UTR 35.0%
AATGAGAATGACAACTTGGA+AGG + tig0026539:21213-21232 Msa1429640:three_prime_UTR 35.0%
GATACATCTGACTATGATGT+GGG + tig0026539:21274-21293 Msa1429640:three_prime_UTR 35.0%
CTATGATGTGGGATTTGATT+TGG + tig0026539:21285-21304 Msa1429640:three_prime_UTR 35.0%
!! AAAGAATGGACTATTCCCTA+TGG - tig0026539:21376-21395 None:intergenic 35.0%
TTCTCTTATGAGATATTGGG+GGG + tig0026539:21411-21430 Msa1429640:three_prime_UTR 35.0%
AGGAAAGACAAAAACTTGGG+TGG - tig0026539:20165-20184 None:intergenic 40.0%
CAACTCGGAGAAATCTCAAT+AGG + tig0026539:20209-20228 Msa1429640:five_prime_UTR 40.0%
CTTCAATCTTTCACCTTCAC+CGG - tig0026539:20346-20365 None:intergenic 40.0%
TGATTCCCCCACTTCAAATA+AGG - tig0026539:20393-20412 None:intergenic 40.0%
TTTCAGGATTCTTCTGACAC+CGG - tig0026539:20425-20444 None:intergenic 40.0%
AGTCTGTTCATCCTATTCTC+CGG - tig0026539:20477-20496 None:intergenic 40.0%
ATGAACAGACTGAGAATCGA+TGG + tig0026539:20486-20505 Msa1429640:CDS 40.0%
TTTGATCTGAAGGTTGAAGC+TGG + tig0026539:20549-20568 Msa1429640:CDS 40.0%
AAGAAGGAGAAAGAGAATGG+TGG + tig0026539:20594-20613 Msa1429640:CDS 40.0%
AGGTGTGGAATTTGAGAACA+AGG + tig0026539:20652-20671 Msa1429640:CDS 40.0%
TGGAATTTGAGAACAAGGAG+AGG + tig0026539:20657-20676 Msa1429640:CDS 40.0%
! AGAACAAGGAGAGGTTTTTC+TGG + tig0026539:20666-20685 Msa1429640:CDS 40.0%
!! AGAGGTTTTTCTGGAGAGAA+TGG + tig0026539:20675-20694 Msa1429640:CDS 40.0%
GTAAACTTCACCTTCTTCTC+AGG - tig0026539:20792-20811 None:intergenic 40.0%
GAAGATTTCATCACGATGAC+TGG + tig0026539:20840-20859 Msa1429640:CDS 40.0%
TGTTCCTGAGAATAATGGCA+AGG + tig0026539:21081-21100 Msa1429640:CDS 40.0%
GGATACATCTGACTATGATG+TGG + tig0026539:21273-21292 Msa1429640:three_prime_UTR 40.0%
AGGAAAGACAAAAACTTGGG+TGG - tig0026539:20165-20184 None:intergenic 40.0%
CAACTCGGAGAAATCTCAAT+AGG + tig0026539:20209-20228 Msa1429640:five_prime_UTR 40.0%
CTTCAATCTTTCACCTTCAC+CGG - tig0026539:20346-20365 None:intergenic 40.0%
TGATTCCCCCACTTCAAATA+AGG - tig0026539:20393-20412 None:intergenic 40.0%
TTTCAGGATTCTTCTGACAC+CGG - tig0026539:20425-20444 None:intergenic 40.0%
AGTCTGTTCATCCTATTCTC+CGG - tig0026539:20477-20496 None:intergenic 40.0%
ATGAACAGACTGAGAATCGA+TGG + tig0026539:20486-20505 Msa1429640:CDS 40.0%
TTTGATCTGAAGGTTGAAGC+TGG + tig0026539:20549-20568 Msa1429640:CDS 40.0%
AAGAAGGAGAAAGAGAATGG+TGG + tig0026539:20594-20613 Msa1429640:CDS 40.0%
AGGTGTGGAATTTGAGAACA+AGG + tig0026539:20652-20671 Msa1429640:CDS 40.0%
TGGAATTTGAGAACAAGGAG+AGG + tig0026539:20657-20676 Msa1429640:CDS 40.0%
! AGAACAAGGAGAGGTTTTTC+TGG + tig0026539:20666-20685 Msa1429640:CDS 40.0%
!! AGAGGTTTTTCTGGAGAGAA+TGG + tig0026539:20675-20694 Msa1429640:CDS 40.0%
GTAAACTTCACCTTCTTCTC+AGG - tig0026539:20792-20811 None:intergenic 40.0%
GAAGATTTCATCACGATGAC+TGG + tig0026539:20840-20859 Msa1429640:CDS 40.0%
TGTTCCTGAGAATAATGGCA+AGG + tig0026539:21081-21100 Msa1429640:CDS 40.0%
GGATACATCTGACTATGATG+TGG + tig0026539:21273-21292 Msa1429640:three_prime_UTR 40.0%
TCTGGTGAAGAAGAAGAGAG+TGG + tig0026539:20300-20319 Msa1429640:CDS 45.0%
!! AGAAGAGAGTGGTGCTGTTA+TGG + tig0026539:20311-20330 Msa1429640:CDS 45.0%
!! GAAGAGAGTGGTGCTGTTAT+GGG + tig0026539:20312-20331 Msa1429640:CDS 45.0%
!! AGTGGTGCTGTTATGGGTAA+TGG + tig0026539:20318-20337 Msa1429640:CDS 45.0%
! GCTGTTATGGGTAATGGTTC+CGG + tig0026539:20324-20343 Msa1429640:CDS 45.0%
AACAGACTGAGAATCGATGG+TGG + tig0026539:20489-20508 Msa1429640:CDS 45.0%
! AGAATCGATGGTGGTGATGA+TGG + tig0026539:20498-20517 Msa1429640:CDS 45.0%
! GAATCGATGGTGGTGATGAT+GGG + tig0026539:20499-20518 Msa1429640:CDS 45.0%
ATCTGAAGGTTGAAGCTGGT+AGG + tig0026539:20553-20572 Msa1429640:CDS 45.0%
AAGGAGAAAGAGAATGGTGG+TGG + tig0026539:20597-20616 Msa1429640:CDS 45.0%
!! AAAGAGAATGGTGGTGGAGA+AGG + tig0026539:20603-20622 Msa1429640:CDS 45.0%
!! AAGAGAATGGTGGTGGAGAA+GGG + tig0026539:20604-20623 Msa1429640:CDS 45.0%
TCGCCGTTAAGGAATGGTAA+CGG + tig0026539:20738-20757 Msa1429640:CDS 45.0%
CTGGTCAAAAGCCTCATAGA+AGG + tig0026539:20859-20878 Msa1429640:CDS 45.0%
!! CTCTTTTTGGGCCTTCTATG+AGG - tig0026539:20873-20892 None:intergenic 45.0%
CAACCACATACCAGGGAAAA+GGG - tig0026539:21023-21042 None:intergenic 45.0%
ACAACCACATACCAGGGAAA+AGG - tig0026539:21024-21043 None:intergenic 45.0%
TCTCAGACAACCACATACCA+GGG - tig0026539:21030-21049 None:intergenic 45.0%
ATCTCAGACAACCACATACC+AGG - tig0026539:21031-21050 None:intergenic 45.0%
CACACCTTGCCATTATTCTC+AGG - tig0026539:21088-21107 None:intergenic 45.0%
TCCTAGCAGTCAGTTAACAC+AGG - tig0026539:21239-21258 None:intergenic 45.0%
TGTTAACTGACTGCTAGGAG+TGG + tig0026539:21240-21259 Msa1429640:three_prime_UTR 45.0%
TCTTATGAGATATTGGGGGG+AGG + tig0026539:21414-21433 Msa1429640:three_prime_UTR 45.0%
CTTATGAGATATTGGGGGGA+GGG + tig0026539:21415-21434 Msa1429640:three_prime_UTR 45.0%
TCTGGTGAAGAAGAAGAGAG+TGG + tig0026539:20300-20319 Msa1429640:CDS 45.0%
!! AGAAGAGAGTGGTGCTGTTA+TGG + tig0026539:20311-20330 Msa1429640:CDS 45.0%
!! GAAGAGAGTGGTGCTGTTAT+GGG + tig0026539:20312-20331 Msa1429640:CDS 45.0%
!! AGTGGTGCTGTTATGGGTAA+TGG + tig0026539:20318-20337 Msa1429640:CDS 45.0%
! GCTGTTATGGGTAATGGTTC+CGG + tig0026539:20324-20343 Msa1429640:CDS 45.0%
AACAGACTGAGAATCGATGG+TGG + tig0026539:20489-20508 Msa1429640:CDS 45.0%
! AGAATCGATGGTGGTGATGA+TGG + tig0026539:20498-20517 Msa1429640:CDS 45.0%
! GAATCGATGGTGGTGATGAT+GGG + tig0026539:20499-20518 Msa1429640:CDS 45.0%
ATCTGAAGGTTGAAGCTGGT+AGG + tig0026539:20553-20572 Msa1429640:CDS 45.0%
AAGGAGAAAGAGAATGGTGG+TGG + tig0026539:20597-20616 Msa1429640:CDS 45.0%
!! AAAGAGAATGGTGGTGGAGA+AGG + tig0026539:20603-20622 Msa1429640:CDS 45.0%
!! AAGAGAATGGTGGTGGAGAA+GGG + tig0026539:20604-20623 Msa1429640:CDS 45.0%
TCGCCGTTAAGGAATGGTAA+CGG + tig0026539:20738-20757 Msa1429640:CDS 45.0%
CTGGTCAAAAGCCTCATAGA+AGG + tig0026539:20859-20878 Msa1429640:CDS 45.0%
!! CTCTTTTTGGGCCTTCTATG+AGG - tig0026539:20873-20892 None:intergenic 45.0%
CAACCACATACCAGGGAAAA+GGG - tig0026539:21023-21042 None:intergenic 45.0%
ACAACCACATACCAGGGAAA+AGG - tig0026539:21024-21043 None:intergenic 45.0%
TCTCAGACAACCACATACCA+GGG - tig0026539:21030-21049 None:intergenic 45.0%
ATCTCAGACAACCACATACC+AGG - tig0026539:21031-21050 None:intergenic 45.0%
CACACCTTGCCATTATTCTC+AGG - tig0026539:21088-21107 None:intergenic 45.0%
TCCTAGCAGTCAGTTAACAC+AGG - tig0026539:21239-21258 None:intergenic 45.0%
TGTTAACTGACTGCTAGGAG+TGG + tig0026539:21240-21259 Msa1429640:three_prime_UTR 45.0%
TCTTATGAGATATTGGGGGG+AGG + tig0026539:21414-21433 Msa1429640:three_prime_UTR 45.0%
CTTATGAGATATTGGGGGGA+GGG + tig0026539:21415-21434 Msa1429640:three_prime_UTR 45.0%
! ATGGGTAATGGTTCCGGTGA+AGG + tig0026539:20330-20349 Msa1429640:CDS 50.0%
CTGTTCATCCTATTCTCCGG+CGG - tig0026539:20474-20493 None:intergenic 50.0%
GGATCGATGCTATGCGTGAA+AGG + tig0026539:20520-20539 Msa1429640:CDS 50.0%
GGTTTGTCGCCGTTAAGGAA+TGG + tig0026539:20732-20751 Msa1429640:CDS 50.0%
CCGCCGTTACCATTCCTTAA+CGG - tig0026539:20744-20763 None:intergenic 50.0%
!! CCGTTAAGGAATGGTAACGG+CGG + tig0026539:20741-20760 Msa1429640:CDS 50.0%
!! TTAAGGAATGGTAACGGCGG+TGG + tig0026539:20744-20763 Msa1429640:CDS 50.0%
AACGGCGGTGGTAAGTTAAG+AGG + tig0026539:20756-20775 Msa1429640:CDS 50.0%
GGTGGTAAGTTAAGAGGTGG+TGG + tig0026539:20762-20781 Msa1429640:CDS 50.0%
TGGTGGTTGTCCTGAGAAGA+AGG + tig0026539:20779-20798 Msa1429640:CDS 50.0%
! TTTGTGCAGACCCTTTTCCC+TGG + tig0026539:21010-21029 Msa1429640:intron 50.0%
! AGACCCTTTTCCCTGGTATG+TGG + tig0026539:21017-21036 Msa1429640:intron 50.0%
GCCTGTGTTAACTGACTGCT+AGG + tig0026539:21235-21254 Msa1429640:three_prime_UTR 50.0%
GCTAGGAGTGGTAACAGCTT+AGG + tig0026539:21252-21271 Msa1429640:three_prime_UTR 50.0%
! ATGGGTAATGGTTCCGGTGA+AGG + tig0026539:20330-20349 Msa1429640:CDS 50.0%
CTGTTCATCCTATTCTCCGG+CGG - tig0026539:20474-20493 None:intergenic 50.0%
GGATCGATGCTATGCGTGAA+AGG + tig0026539:20520-20539 Msa1429640:CDS 50.0%
GGTTTGTCGCCGTTAAGGAA+TGG + tig0026539:20732-20751 Msa1429640:CDS 50.0%
CCGCCGTTACCATTCCTTAA+CGG - tig0026539:20744-20763 None:intergenic 50.0%
!! CCGTTAAGGAATGGTAACGG+CGG + tig0026539:20741-20760 Msa1429640:CDS 50.0%
!! TTAAGGAATGGTAACGGCGG+TGG + tig0026539:20744-20763 Msa1429640:CDS 50.0%
AACGGCGGTGGTAAGTTAAG+AGG + tig0026539:20756-20775 Msa1429640:CDS 50.0%
GGTGGTAAGTTAAGAGGTGG+TGG + tig0026539:20762-20781 Msa1429640:CDS 50.0%
TGGTGGTTGTCCTGAGAAGA+AGG + tig0026539:20779-20798 Msa1429640:CDS 50.0%
! TTTGTGCAGACCCTTTTCCC+TGG + tig0026539:21010-21029 Msa1429640:intron 50.0%
! AGACCCTTTTCCCTGGTATG+TGG + tig0026539:21017-21036 Msa1429640:intron 50.0%
GCCTGTGTTAACTGACTGCT+AGG + tig0026539:21235-21254 Msa1429640:three_prime_UTR 50.0%
GCTAGGAGTGGTAACAGCTT+AGG + tig0026539:21252-21271 Msa1429640:three_prime_UTR 50.0%
TGGGTGGCTTGAAAACTCGC+CGG - tig0026539:20149-20168 None:intergenic 55.0%
! GACGGATCGTCACCGTTTTC+AGG - tig0026539:20441-20460 None:intergenic 55.0%
AAACGGTGACGATCCGTCGA+TGG + tig0026539:20443-20462 Msa1429640:CDS 55.0%
CTTAACGGCGACAAACCGAG+AGG - tig0026539:20729-20748 None:intergenic 55.0%
! CTCTCGGTTTGTCGCCGTTA+AGG + tig0026539:20727-20746 Msa1429640:CDS 55.0%
GGCGGTGGTAAGTTAAGAGG+TGG + tig0026539:20759-20778 Msa1429640:CDS 55.0%
TGGGTGGCTTGAAAACTCGC+CGG - tig0026539:20149-20168 None:intergenic 55.0%
! GACGGATCGTCACCGTTTTC+AGG - tig0026539:20441-20460 None:intergenic 55.0%
AAACGGTGACGATCCGTCGA+TGG + tig0026539:20443-20462 Msa1429640:CDS 55.0%
CTTAACGGCGACAAACCGAG+AGG - tig0026539:20729-20748 None:intergenic 55.0%
! CTCTCGGTTTGTCGCCGTTA+AGG + tig0026539:20727-20746 Msa1429640:CDS 55.0%
GGCGGTGGTAAGTTAAGAGG+TGG + tig0026539:20759-20778 Msa1429640:CDS 55.0%
GGGTGGCTTGAAAACTCGCC+GGG - tig0026539:20148-20167 None:intergenic 60.0%
GGGGGAATCAGCGTTCTCTC+CGG + tig0026539:20403-20422 Msa1429640:CDS 60.0%
!! TGGTGTCACCGCCGGAGAAT+AGG + tig0026539:20463-20482 Msa1429640:CDS 60.0%
TGGTGGAGAAGGGTCGTCGA+CGG + tig0026539:20614-20633 Msa1429640:CDS 60.0%
GGGTGGCTTGAAAACTCGCC+GGG - tig0026539:20148-20167 None:intergenic 60.0%
GGGGGAATCAGCGTTCTCTC+CGG + tig0026539:20403-20422 Msa1429640:CDS 60.0%
!! TGGTGTCACCGCCGGAGAAT+AGG + tig0026539:20463-20482 Msa1429640:CDS 60.0%
TGGTGGAGAAGGGTCGTCGA+CGG + tig0026539:20614-20633 Msa1429640:CDS 60.0%
!! TCCGTCGATGGTGTCACCGC+CGG + tig0026539:20455-20474 Msa1429640:CDS 65.0%
TCCGGCGGTGACACCATCGA+CGG - tig0026539:20459-20478 None:intergenic 65.0%
AGAAGGGTCGTCGACGGCTG+CGG + tig0026539:20620-20639 Msa1429640:CDS 65.0%
!! TCCGTCGATGGTGTCACCGC+CGG + tig0026539:20455-20474 Msa1429640:CDS 65.0%
TCCGGCGGTGACACCATCGA+CGG - tig0026539:20459-20478 None:intergenic 65.0%
AGAAGGGTCGTCGACGGCTG+CGG + tig0026539:20620-20639 Msa1429640:CDS 65.0%
CTGCGGCGGGAGAGAAGGTG+TGG + tig0026539:20637-20656 Msa1429640:CDS 70.0%
CTGCGGCGGGAGAGAAGGTG+TGG + tig0026539:20637-20656 Msa1429640:CDS 70.0%
AGGGTCGTCGACGGCTGCGG+CGG + tig0026539:20623-20642 Msa1429640:CDS 75.0%
GACGGCTGCGGCGGGAGAGA+AGG + tig0026539:20632-20651 Msa1429640:CDS 75.0%
AGGGTCGTCGACGGCTGCGG+CGG + tig0026539:20623-20642 Msa1429640:CDS 75.0%
GACGGCTGCGGCGGGAGAGA+AGG + tig0026539:20632-20651 Msa1429640:CDS 75.0%
! GGGTCGTCGACGGCTGCGGC+GGG + tig0026539:20624-20643 Msa1429640:CDS 80.0%
! GGGTCGTCGACGGCTGCGGC+GGG + tig0026539:20624-20643 Msa1429640:CDS 80.0%
Chromosome Type Strat End Strand Name
tig0026539 gene 20125 21516 20125 ID=Msa1429640;Name=Msa1429640
tig0026539 mRNA 20125 21516 20125 ID=Msa1429640-mRNA-1;Parent=Msa1429640;Name=Msa1429640-mRNA-1;_AED=0.04;_eAED=0.04;_QI=166|1|1|1|1|1|3|311|239
tig0026539 exon 20125 20920 20125 ID=Msa1429640-mRNA-1:exon:6621;Parent=Msa1429640-mRNA-1
tig0026539 exon 21019 21102 21019 ID=Msa1429640-mRNA-1:exon:6622;Parent=Msa1429640-mRNA-1
tig0026539 exon 21200 21516 21200 ID=Msa1429640-mRNA-1:exon:6623;Parent=Msa1429640-mRNA-1
tig0026539 five_prime_UTR 20125 20290 20125 ID=Msa1429640-mRNA-1:five_prime_utr;Parent=Msa1429640-mRNA-1
tig0026539 CDS 20291 20920 20291 ID=Msa1429640-mRNA-1:cds;Parent=Msa1429640-mRNA-1
tig0026539 CDS 21019 21102 21019 ID=Msa1429640-mRNA-1:cds;Parent=Msa1429640-mRNA-1
tig0026539 CDS 21200 21205 21200 ID=Msa1429640-mRNA-1:cds;Parent=Msa1429640-mRNA-1
tig0026539 three_prime_UTR 21206 21516 21206 ID=Msa1429640-mRNA-1:three_prime_utr;Parent=Msa1429640-mRNA-1
Gene Sequence

>Msa1429640

ATGGTGTTTTCTGGTGAAGAAGAAGAGAGTGGTGCTGTTATGGGTAATGGTTCCGGTGAAGGTGAAAGATTGAAGCATCCTTTTCACAACTTCCATTTTCCTTATTTGAAGTGGGGGAATCAGCGTTCTCTCCGGTGTCAGAAGAATCCTGAAAACGGTGACGATCCGTCGATGGTGTCACCGCCGGAGAATAGGATGAACAGACTGAGAATCGATGGTGGTGATGATGGGATCGATGCTATGCGTGAAAGGTTGATGTTTGATCTGAAGGTTGAAGCTGGTAGGATGAAAGATGCGATTTTGAAGAAGGAGAAAGAGAATGGTGGTGGAGAAGGGTCGTCGACGGCTGCGGCGGGAGAGAAGGTGTGGAATTTGAGAACAAGGAGAGGTTTTTCTGGAGAGAATGGAAAGGGATTGAAGATTGATGAGAAAAAACCTCTCGGTTTGTCGCCGTTAAGGAATGGTAACGGCGGTGGTAAGTTAAGAGGTGGTGGTTGTCCTGAGAAGAAGGTGAAGTTTACTGTGTCATTGATGAAGAAGGAGATTGAAGAAGATTTCATCACGATGACTGGTCAAAAGCCTCATAGAAGGCCCAAAAAGAGACCTAGAAATGTTCAGAAGCAAATGGATACCCTTTTCCCTGGTATGTGGTTGTCTGAGATTAATGCTGATTCCTATAAGGTTCCTGATGATGTTCCTGAGAATAATGGCAAGAGGTAG
Protein sequence

>Msa1429640

MVFSGEEEESGAVMGNGSGEGERLKHPFHNFHFPYLKWGNQRSLRCQKNPENGDDPSMVSPPENRMNRLRIDGGDDGIDAMRERLMFDLKVEAGRMKDAILKKEKENGGGEGSSTAAAGEKVWNLRTRRGFSGENGKGLKIDEKKPLGLSPLRNGNGGGKLRGGGCPEKKVKFTVSLMKKEIEEDFITMTGQKPHRRPKKRPRNVQKQMDTLFPGMWLSEINADSYKVPDDVPENNGKR*