AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0830920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0830920 MtrunA17_Chr5g0396661 81.735 219 7 1 1 219 1 186 1.06e-121 342
Msa0830920 MtrunA17_Chr8g0388141 63.470 219 47 1 1 219 62 247 1.47e-90 266
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0830920 AT4G23895.4 60.274 219 52 2 1 219 60 243 1.46e-82 246
Msa0830920 AT4G23895.2 60.274 219 52 2 1 219 60 243 1.46e-82 246
Msa0830920 AT4G23895.1 60.274 219 52 2 1 219 60 243 1.46e-82 246
Msa0830920 AT4G23895.3 61.722 209 45 2 1 209 60 233 3.76e-78 242

Find 76 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCTGTATTTCTCACTTTA+AGG 0.099261 5_4:+3935298 Msa0830920:CDS
TCGCACAATCTAGCTTATTT+TGG 0.119039 5_4:+3934222 Msa0830920:five_prime_UTR
TCCTCTAATTCAAAATTTAA+TGG 0.168766 5_4:-3936187 None:intergenic
CACGCTTAATACTTGGTAAT+TGG 0.189394 5_4:-3935672 None:intergenic
TCCTCGCTTTGTTGTTCTTC+AGG 0.207772 5_4:+3935212 Msa0830920:CDS
ACTAATGAACTTTGCTAGTT+TGG 0.230127 5_4:+3936210 Msa0830920:three_prime_UTR
AATAGAGACTGATGATTATT+TGG 0.240870 5_4:+3936132 Msa0830920:three_prime_UTR
TGAGTTCCAGATTCAGTAAT+AGG 0.270097 5_4:-3934133 None:intergenic
ATCAGAACTTGTTGATTTAG+AGG 0.291898 5_4:+3934477 Msa0830920:CDS
CCTCGCTTTGTTGTTCTTCA+GGG 0.314121 5_4:+3935213 Msa0830920:CDS
CCTCTAATTCAAAATTTAAT+GGG 0.325775 5_4:-3936186 None:intergenic
GGCATTTCCTTGAGAACTTA+AGG 0.331835 5_4:+3934051 Msa0830920:five_prime_UTR
AAGGCACCTATTACTGAATC+TGG 0.344705 5_4:+3934127 Msa0830920:five_prime_UTR
GATGTTTCAGGATTATTTAT+CGG 0.365961 5_4:+3933992 Msa0830920:intron
GGTCACTTCTGACTTATTCC+AGG 0.382654 5_4:+3936153 Msa0830920:three_prime_UTR
CCATCTTCACGCTTAATACT+TGG 0.382946 5_4:-3935679 None:intergenic
TATAACTAACTTAACTGTTC+AGG 0.384599 5_4:-3936266 None:intergenic
TTGCGGTCAGCTCGTCTATC+CGG 0.396043 5_4:+3935008 Msa0830920:CDS
TTGAAATTGTTTACACTTCG+AGG 0.404814 5_4:+3934294 Msa0830920:CDS
CAGAGATGGACAGAGCTTCC+AGG 0.406603 5_4:+3935147 Msa0830920:intron
CTCGAGCAGTTCTAAGATAC+AGG 0.424024 5_4:+3935756 Msa0830920:CDS
TCTGCAATCGGACTGCAAAC+TGG 0.436293 5_4:+3935953 Msa0830920:CDS
TCTACATCGGTATCATCAAT+AGG 0.447712 5_4:-3935180 None:intergenic
ACCTATCCAGAGGAAGATAA+TGG 0.451212 5_4:-3934027 None:intergenic
TTCATATATTTGTTGAGTAC+AGG 0.456033 5_4:+3935246 Msa0830920:CDS
TTATCGCCATTATCTTCCTC+TGG 0.456512 5_4:+3934021 Msa0830920:five_prime_UTR
ACTTGTTGATTTAGAGGAGA+AGG 0.457112 5_4:+3934483 Msa0830920:CDS
TTAGTTAGTTGTACCTGGCC+TGG 0.458537 5_4:-3933195 None:intergenic
AATGGATTTCTTTCATTCGA+AGG 0.473694 5_4:-3933112 None:intergenic
TTATCTTCCTCTGGATAGGT+TGG 0.480663 5_4:+3934030 Msa0830920:five_prime_UTR
CTTCTCTTCTTAACACGGCC+AGG 0.481417 5_4:+3933177 Msa0830920:five_prime_UTR
ATGTCGTGTTAAAATACAAA+AGG 0.492182 5_4:-3935716 None:intergenic
GGTTCGGATGTATAAATATC+CGG 0.494584 5_4:-3935027 None:intergenic
AGAATATAAGAGAGATTCAT+CGG 0.494851 5_4:-3935328 None:intergenic
CTTGGTGTTCTTCAAGTCCT+CGG 0.496975 5_4:-3934258 None:intergenic
CCAAGTATTAAGCGTGAAGA+TGG 0.499854 5_4:+3935679 Msa0830920:CDS
AAGTAGGTAGATACTACTAA+TGG 0.500409 5_4:-3933130 None:intergenic
CTTCTTAACACGGCCAGGCC+AGG 0.500500 5_4:+3933182 Msa0830920:five_prime_UTR
TTACATCTATATCGAACCAT+TGG 0.502885 5_4:-3935994 None:intergenic
ATGATACCGATGTAGATGAT+TGG 0.507290 5_4:+3935187 Msa0830920:CDS
GAGCCCTAGTAGTGGTAGTC+GGG 0.510554 5_4:+3934326 Msa0830920:CDS
CTTCTCTTCTCTTCTTAACA+CGG 0.512426 5_4:+3933172 Msa0830920:five_prime_UTR
TAGTAGTGGTAGTCGGGAAA+AGG 0.515903 5_4:+3934332 Msa0830920:CDS
TTCAACATGAAAGCAGCCCT+TGG 0.520437 5_4:-3934276 None:intergenic
GTGTTCTTCAAGTCCTCGGA+TGG 0.524855 5_4:-3934254 None:intergenic
AAGAGAGATTCATCGGGTAA+AGG 0.527630 5_4:-3935321 None:intergenic
GTGGTTATCAGCTCTGCAAT+CGG 0.533434 5_4:+3935941 Msa0830920:CDS
TACATCTATATCGAACCATT+GGG 0.534421 5_4:-3935993 None:intergenic
TTACACTTCGAGGGCTGCCA+TGG 0.535812 5_4:+3934304 Msa0830920:CDS
CAGATGACCTTAAGTTCTCA+AGG 0.536063 5_4:-3934058 None:intergenic
GGACGAAAACGAAGGTAAGT+AGG 0.543421 5_4:-3933146 None:intergenic
TCAATAGGTGGAGATTCTCC+TGG 0.549524 5_4:-3935165 None:intergenic
TTGGGCCTTGGTGCCATCCG+AGG 0.549919 5_4:+3934241 Msa0830920:five_prime_UTR
TGAAATTGTTTACACTTCGA+GGG 0.554877 5_4:+3934295 Msa0830920:CDS
TTAATGGGTCAATCAAGTCC+TGG 0.561085 5_4:-3936171 None:intergenic
TGTACAAGAAATAATAGTCA+TGG 0.573002 5_4:-3936091 None:intergenic
GAATATAAGAGAGATTCATC+GGG 0.575538 5_4:-3935327 None:intergenic
GCCATTATCTTCCTCTGGAT+AGG 0.579320 5_4:+3934026 Msa0830920:five_prime_UTR
GGAGCCCTAGTAGTGGTAGT+CGG 0.588289 5_4:+3934325 Msa0830920:CDS
TGTTGATTTAGAGGAGAAGG+AGG 0.603598 5_4:+3934486 Msa0830920:CDS
CTACCACTACTAGGGCTCCA+TGG 0.603848 5_4:-3934321 None:intergenic
AATAAAGATCAACACTCTGA+AGG 0.618147 5_4:+3934192 Msa0830920:five_prime_UTR
TTTCCCGACTACCACTACTA+GGG 0.626466 5_4:-3934329 None:intergenic
TCTGATGCATCAACTCCCAA+TGG 0.627776 5_4:+3935978 Msa0830920:CDS
CCCTGAAGAACAACAAAGCG+AGG 0.633323 5_4:-3935213 None:intergenic
TGTAGAATATCTTGAGAACA+AGG 0.639246 5_4:+3934108 Msa0830920:five_prime_UTR
AGAAGAGAGGACGAAAACGA+AGG 0.639661 5_4:-3933154 None:intergenic
CTGCCATGGAGCCCTAGTAG+TGG 0.643912 5_4:+3934318 Msa0830920:CDS
TTAAGAAGAGAAGAGAAGAG+AGG 0.655292 5_4:-3933167 None:intergenic
GATAGAAGTGTTGAGTCGTG+AGG 0.659394 5_4:-3935631 None:intergenic
AGGAAGCCAATCATCTACAT+CGG 0.663664 5_4:-3935193 None:intergenic
CAAGTCCTCGGATGGCACCA+AGG 0.664072 5_4:-3934246 None:intergenic
ACATCGGTATCATCAATAGG+TGG 0.664944 5_4:-3935177 None:intergenic
GAGGACTTGAAGAACACCAA+GGG 0.673825 5_4:+3934260 Msa0830920:five_prime_UTR
GGAAATGCCAACCTATCCAG+AGG 0.683785 5_4:-3934037 None:intergenic
CGAGGACTTGAAGAACACCA+AGG 0.708009 5_4:+3934259 Msa0830920:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAGGTTAAATTTGT+AGG - chr5_4:3933282-3933301 None:intergenic 15.0%
!! TATGCAATATGATATTTAAT+TGG + chr5_4:3933477-3933496 Msa0830920:intron 15.0%
!!! TACTTTTCTTCTTTAAATTA+CGG - chr5_4:3933788-3933807 None:intergenic 15.0%
!!! AAAATATTGATGAAATTTTG+CGG + chr5_4:3934991-3935010 Msa0830920:CDS 15.0%
!! AAAACAAAAGTAATAAAGAG+AGG - chr5_4:3933249-3933268 None:intergenic 20.0%
!!! TTTAACCTTTTTTATTCTCT+TGG + chr5_4:3933287-3933306 Msa0830920:intron 20.0%
!!! TAAGTTGATTAATGAAGTTT+AGG + chr5_4:3933342-3933361 Msa0830920:intron 20.0%
!!! AAGTTGATTAATGAAGTTTA+GGG + chr5_4:3933343-3933362 Msa0830920:intron 20.0%
!! ATAAACTGAATATGTAGAAT+CGG - chr5_4:3933726-3933745 None:intergenic 20.0%
!!! CTACAAAATATAATTGACTT+TGG - chr5_4:3934422-3934441 None:intergenic 20.0%
!!! CAAAGTCAATTATATTTTGT+AGG + chr5_4:3934420-3934439 Msa0830920:intron 20.0%
!!! TTGATTTTTTTATTTGACAC+AGG + chr5_4:3934964-3934983 Msa0830920:intron 20.0%
!! AAATAAACTAATGAAGGTTT+GGG - chr5_4:3935064-3935083 None:intergenic 20.0%
!! AAAATAAACTAATGAAGGTT+TGG - chr5_4:3935065-3935084 None:intergenic 20.0%
!! ATAAGGATAAAAAAATAGAG+AGG - chr5_4:3935452-3935471 None:intergenic 20.0%
!!! CTCTTTTTACCAATATTTTT+CGG + chr5_4:3935554-3935573 Msa0830920:intron 20.0%
!! TAATCAGATATTCTTAAGTT+AGG - chr5_4:3935786-3935805 None:intergenic 20.0%
!! CCTCTAATTCAAAATTTAAT+GGG - chr5_4:3936189-3936208 None:intergenic 20.0%
!! TCCTCTAATTCAAAATTTAA+TGG - chr5_4:3936190-3936209 None:intergenic 20.0%
! ACAATCCAAGAGAATAAAAA+AGG - chr5_4:3933295-3933314 None:intergenic 25.0%
!! TTTTATTCTCTTGGATTGTT+TGG + chr5_4:3933296-3933315 Msa0830920:intron 25.0%
! AGTTGAGAATCAGAATATTT+AGG + chr5_4:3933414-3933433 Msa0830920:intron 25.0%
!! ATAGTGGAACAGTTATTAAT+TGG - chr5_4:3933515-3933534 None:intergenic 25.0%
! TAGTTTAAAAGCAAGTTTGT+CGG + chr5_4:3933623-3933642 Msa0830920:intron 25.0%
!! AAAGTCCAAAAATCCTTTTA+GGG - chr5_4:3933855-3933874 None:intergenic 25.0%
!! TAAAGTCCAAAAATCCTTTT+AGG - chr5_4:3933856-3933875 None:intergenic 25.0%
!! GACTTTATATTTTGCAAGAT+TGG + chr5_4:3933869-3933888 Msa0830920:intron 25.0%
! AATATGGTAGTACTTAGATA+CGG + chr5_4:3933897-3933916 Msa0830920:intron 25.0%
! GATGTTTCAGGATTATTTAT+CGG + chr5_4:3933992-3934011 Msa0830920:intron 25.0%
! TGAAGAACTACAAAACAATA+AGG - chr5_4:3934810-3934829 None:intergenic 25.0%
! AGCAAAAAAGAATCACTATA+TGG - chr5_4:3934840-3934859 None:intergenic 25.0%
!!! ATTCTTTTTTGCTGTTACAA+AGG + chr5_4:3934847-3934866 Msa0830920:intron 25.0%
!!! TTTTTTTATTTGACACAGGT+TGG + chr5_4:3934968-3934987 Msa0830920:intron 25.0%
! GATGCAAAATAAACTAATGA+AGG - chr5_4:3935070-3935089 None:intergenic 25.0%
!!! TCATTTTTTATCTACAGAGA+TGG + chr5_4:3935133-3935152 Msa0830920:intron 25.0%
!! TTCATATATTTGTTGAGTAC+AGG + chr5_4:3935246-3935265 Msa0830920:CDS 25.0%
! AGAATATAAGAGAGATTCAT+CGG - chr5_4:3935331-3935350 None:intergenic 25.0%
! CGAAAAATATTGGTAAAAAG+AGG - chr5_4:3935556-3935575 None:intergenic 25.0%
! ATGTCGTGTTAAAATACAAA+AGG - chr5_4:3935719-3935738 None:intergenic 25.0%
!!! TTTTGTATTTTAACACGACA+TGG + chr5_4:3935718-3935737 Msa0830920:CDS 25.0%
!!! TCTTTTATTCCTTTTTAAGG+TGG + chr5_4:3935914-3935933 Msa0830920:intron 25.0%
! TGTACAAGAAATAATAGTCA+TGG - chr5_4:3936094-3936113 None:intergenic 25.0%
! AATAGAGACTGATGATTATT+TGG + chr5_4:3936132-3936151 Msa0830920:three_prime_UTR 25.0%
!! CCCATTAAATTTTGAATTAG+AGG + chr5_4:3936186-3936205 Msa0830920:three_prime_UTR 25.0%
! AATGGATTTCTTTCATTCGA+AGG - chr5_4:3933115-3933134 None:intergenic 30.0%
AAGTAGGTAGATACTACTAA+TGG - chr5_4:3933133-3933152 None:intergenic 30.0%
GAGGAAAAAGAAAGAGTTTA+TGG - chr5_4:3933230-3933249 None:intergenic 30.0%
TGAGAATCAGAATATTTAGG+TGG + chr5_4:3933417-3933436 Msa0830920:intron 30.0%
GAGAATCAGAATATTTAGGT+GGG + chr5_4:3933418-3933437 Msa0830920:intron 30.0%
AGAATCAGAATATTTAGGTG+GGG + chr5_4:3933419-3933438 Msa0830920:intron 30.0%
GGAACAGTTATTAATTGGTT+AGG - chr5_4:3933510-3933529 None:intergenic 30.0%
! AATCACCTCTAAAGAAATAG+TGG - chr5_4:3933531-3933550 None:intergenic 30.0%
AGTTTAAAAGCAAGTTTGTC+GGG + chr5_4:3933624-3933643 Msa0830920:intron 30.0%
ATATGGTAGTACTTAGATAC+GGG + chr5_4:3933898-3933917 Msa0830920:intron 30.0%
TGATATTACTATTGGCAACT+TGG - chr5_4:3933932-3933951 None:intergenic 30.0%
TCCTCTAGTGATATTACTAT+TGG - chr5_4:3933940-3933959 None:intergenic 30.0%
TGTAGAATATCTTGAGAACA+AGG + chr5_4:3934108-3934127 Msa0830920:five_prime_UTR 30.0%
! CTGGAACTCATAAATCTTTT+AGG + chr5_4:3934146-3934165 Msa0830920:five_prime_UTR 30.0%
!! ATCTTTTAGGCATTGTTTTC+TGG + chr5_4:3934159-3934178 Msa0830920:five_prime_UTR 30.0%
AATAAAGATCAACACTCTGA+AGG + chr5_4:3934192-3934211 Msa0830920:five_prime_UTR 30.0%
TTGAAATTGTTTACACTTCG+AGG + chr5_4:3934294-3934313 Msa0830920:CDS 30.0%
TGAAATTGTTTACACTTCGA+GGG + chr5_4:3934295-3934314 Msa0830920:CDS 30.0%
! GTTTCTTTTGTCATTGATGA+AGG + chr5_4:3934381-3934400 Msa0830920:intron 30.0%
!! ATCAGAACTTGTTGATTTAG+AGG + chr5_4:3934477-3934496 Msa0830920:CDS 30.0%
GCTGTAACAGTAAAAAGTTA+TGG - chr5_4:3934537-3934556 None:intergenic 30.0%
AAGTGATTGTCTTAGTAGTA+GGG + chr5_4:3934765-3934784 Msa0830920:intron 30.0%
GAATATAAGAGAGATTCATC+GGG - chr5_4:3935330-3935349 None:intergenic 30.0%
AATAGAGAGGAGAGAAAAAA+AGG - chr5_4:3935439-3935458 None:intergenic 30.0%
!! ACCAATATTTTTCGGATGAT+TGG + chr5_4:3935562-3935581 Msa0830920:intron 30.0%
TCATTTACAATTGCTGATGA+AGG + chr5_4:3935588-3935607 Msa0830920:intron 30.0%
CATTTACAATTGCTGATGAA+GGG + chr5_4:3935589-3935608 Msa0830920:intron 30.0%
ATTTACAATTGCTGATGAAG+GGG + chr5_4:3935590-3935609 Msa0830920:intron 30.0%
! CGCTCTTTTATTCCTTTTTA+AGG + chr5_4:3935911-3935930 Msa0830920:intron 30.0%
TACATCTATATCGAACCATT+GGG - chr5_4:3935996-3936015 None:intergenic 30.0%
TTACATCTATATCGAACCAT+TGG - chr5_4:3935997-3936016 None:intergenic 30.0%
! AACATGTTTTACTCCTTGTT+TGG + chr5_4:3936015-3936034 Msa0830920:three_prime_UTR 30.0%
!! ACATGTTTTACTCCTTGTTT+GGG + chr5_4:3936016-3936035 Msa0830920:three_prime_UTR 30.0%
ACTAATGAACTTTGCTAGTT+TGG + chr5_4:3936210-3936229 Msa0830920:three_prime_UTR 30.0%
TTAAGAAGAGAAGAGAAGAG+AGG - chr5_4:3933170-3933189 None:intergenic 35.0%
CTTCTCTTCTCTTCTTAACA+CGG + chr5_4:3933172-3933191 Msa0830920:five_prime_UTR 35.0%
TGTAGTTAGTTAGTTGTACC+TGG - chr5_4:3933203-3933222 None:intergenic 35.0%
GAATCAGAATATTTAGGTGG+GGG + chr5_4:3933420-3933439 Msa0830920:intron 35.0%
ATATTTAGGTGGGGGAAAAT+CGG + chr5_4:3933428-3933447 Msa0830920:intron 35.0%
! CTGTTCCACTATTTCTTTAG+AGG + chr5_4:3933523-3933542 Msa0830920:intron 35.0%
GCTATTAGTAGAAGAAGTGA+TGG + chr5_4:3933675-3933694 Msa0830920:intron 35.0%
!!! AAATCCTTTTAGGGTGTGAT+TGG - chr5_4:3933846-3933865 None:intergenic 35.0%
! TCACACCCTAAAAGGATTTT+TGG + chr5_4:3933847-3933866 Msa0830920:intron 35.0%
!! TGCAAGATTGGTTTGCAATA+TGG + chr5_4:3933881-3933900 Msa0830920:intron 35.0%
GCCAATAGTAATATCACTAG+AGG + chr5_4:3933936-3933955 Msa0830920:intron 35.0%
ACATCAACAATGCTAGAAGT+TGG - chr5_4:3933977-3933996 None:intergenic 35.0%
TCTAGCATTGTTGATGTTTC+AGG + chr5_4:3933980-3933999 Msa0830920:intron 35.0%
!! CACTCTCAAGTTTTAACATC+TGG + chr5_4:3934085-3934104 Msa0830920:five_prime_UTR 35.0%
TGAGTTCCAGATTCAGTAAT+AGG - chr5_4:3934136-3934155 None:intergenic 35.0%
!! TTTTAGGCATTGTTTTCTGG+TGG + chr5_4:3934162-3934181 Msa0830920:five_prime_UTR 35.0%
!! TTTAGGCATTGTTTTCTGGT+GGG + chr5_4:3934163-3934182 Msa0830920:five_prime_UTR 35.0%
!! TCGCACAATCTAGCTTATTT+TGG + chr5_4:3934222-3934241 Msa0830920:five_prime_UTR 35.0%
!! CGCACAATCTAGCTTATTTT+GGG + chr5_4:3934223-3934242 Msa0830920:five_prime_UTR 35.0%
!! ACTTGTTGATTTAGAGGAGA+AGG + chr5_4:3934483-3934502 Msa0830920:CDS 35.0%
! AGTCAAATAGCAGTTAGCTT+TGG + chr5_4:3934633-3934652 Msa0830920:intron 35.0%
!! GTCAAATAGCAGTTAGCTTT+GGG + chr5_4:3934634-3934653 Msa0830920:intron 35.0%
ACATTGAACATGACAAAGCT+AGG + chr5_4:3934705-3934724 Msa0830920:intron 35.0%
CAAGTGATTGTCTTAGTAGT+AGG + chr5_4:3934764-3934783 Msa0830920:intron 35.0%
!! AAAAAGTATTCCCCAGATAG+GGG - chr5_4:3934925-3934944 None:intergenic 35.0%
!! CAAAAAGTATTCCCCAGATA+GGG - chr5_4:3934926-3934945 None:intergenic 35.0%
!! ACAAAAAGTATTCCCCAGAT+AGG - chr5_4:3934927-3934946 None:intergenic 35.0%
GGTTCGGATGTATAAATATC+CGG - chr5_4:3935030-3935049 None:intergenic 35.0%
TCTACATCGGTATCATCAAT+AGG - chr5_4:3935183-3935202 None:intergenic 35.0%
ATGATACCGATGTAGATGAT+TGG + chr5_4:3935187-3935206 Msa0830920:CDS 35.0%
GAGCTGTATTTCTCACTTTA+AGG + chr5_4:3935298-3935317 Msa0830920:CDS 35.0%
GCCAATCATCCGAAAAATAT+TGG - chr5_4:3935566-3935585 None:intergenic 35.0%
TTTACAATTGCTGATGAAGG+GGG + chr5_4:3935591-3935610 Msa0830920:intron 35.0%
! CACGCTTAATACTTGGTAAT+TGG - chr5_4:3935675-3935694 None:intergenic 35.0%
!! CATGTTTTACTCCTTGTTTG+GGG + chr5_4:3936017-3936036 Msa0830920:three_prime_UTR 35.0%
!! ATGTTTTACTCCTTGTTTGG+GGG + chr5_4:3936018-3936037 Msa0830920:three_prime_UTR 35.0%
TCTACTAATAGCAAGCTCAC+CGG - chr5_4:3933667-3933686 None:intergenic 40.0%
ATTGGATGGAGGGTTTAAGA+GGG - chr5_4:3933828-3933847 None:intergenic 40.0%
TTATCGCCATTATCTTCCTC+TGG + chr5_4:3934021-3934040 Msa0830920:five_prime_UTR 40.0%
ACCTATCCAGAGGAAGATAA+TGG - chr5_4:3934030-3934049 None:intergenic 40.0%
TTATCTTCCTCTGGATAGGT+TGG + chr5_4:3934030-3934049 Msa0830920:five_prime_UTR 40.0%
GGCATTTCCTTGAGAACTTA+AGG + chr5_4:3934051-3934070 Msa0830920:five_prime_UTR 40.0%
CAGATGACCTTAAGTTCTCA+AGG - chr5_4:3934061-3934080 None:intergenic 40.0%
! AAGGCACCTATTACTGAATC+TGG + chr5_4:3934127-3934146 Msa0830920:five_prime_UTR 40.0%
!! CATTGTTTTCTGGTGGGAAA+GGG + chr5_4:3934169-3934188 Msa0830920:five_prime_UTR 40.0%
!!! ATCTAGCTTATTTTGGGCCT+TGG + chr5_4:3934229-3934248 Msa0830920:five_prime_UTR 40.0%
! GGAAAAGGTTTGCTTTCATC+AGG + chr5_4:3934347-3934366 Msa0830920:intron 40.0%
!! TGTTGATTTAGAGGAGAAGG+AGG + chr5_4:3934486-3934505 Msa0830920:CDS 40.0%
CATGACAAAGCTAGGATTTC+AGG + chr5_4:3934713-3934732 Msa0830920:intron 40.0%
! TCAGGTTCTAGCCATAGATT+TGG + chr5_4:3934731-3934750 Msa0830920:intron 40.0%
GTGACTAGACTCCAAATCTA+TGG - chr5_4:3934745-3934764 None:intergenic 40.0%
AACACTGAATTTCAACTCCG+AGG - chr5_4:3934884-3934903 None:intergenic 40.0%
AAGGTTTGGGCAGTTAAGTA+CGG - chr5_4:3935051-3935070 None:intergenic 40.0%
GTTAGGTAATGACAAGCAGA+TGG - chr5_4:3935092-3935111 None:intergenic 40.0%
CAATCAGTACTAAGCGTGTT+AGG - chr5_4:3935109-3935128 None:intergenic 40.0%
!! CTAACACGCTTAGTACTGAT+TGG + chr5_4:3935107-3935126 Msa0830920:intron 40.0%
ACATCGGTATCATCAATAGG+TGG - chr5_4:3935180-3935199 None:intergenic 40.0%
AGGAAGCCAATCATCTACAT+CGG - chr5_4:3935196-3935215 None:intergenic 40.0%
AAGAGAGATTCATCGGGTAA+AGG - chr5_4:3935324-3935343 None:intergenic 40.0%
CCATCTTCACGCTTAATACT+TGG - chr5_4:3935682-3935701 None:intergenic 40.0%
CCAAGTATTAAGCGTGAAGA+TGG + chr5_4:3935679-3935698 Msa0830920:CDS 40.0%
!!! TCCTTTTTAAGGTGGATTCG+TGG + chr5_4:3935922-3935941 Msa0830920:intron 40.0%
ACCACGAATCCACCTTAAAA+AGG - chr5_4:3935926-3935945 None:intergenic 40.0%
ATCTCAAATTCCCCCAAACA+AGG - chr5_4:3936031-3936050 None:intergenic 40.0%
TTGTTTGGGGGAATTTGAGA+TGG + chr5_4:3936030-3936049 Msa0830920:three_prime_UTR 40.0%
! TTAATGGGTCAATCAAGTCC+TGG - chr5_4:3936174-3936193 None:intergenic 40.0%
GGACGAAAACGAAGGTAAGT+AGG - chr5_4:3933149-3933168 None:intergenic 45.0%
AGAAGAGAGGACGAAAACGA+AGG - chr5_4:3933157-3933176 None:intergenic 45.0%
TTAGTTAGTTGTACCTGGCC+TGG - chr5_4:3933198-3933217 None:intergenic 45.0%
! TGAAGTTTAGGGCTGATCGA+TGG + chr5_4:3933354-3933373 Msa0830920:intron 45.0%
! GGACTGCTAGTCTAACAATC+CGG + chr5_4:3933645-3933664 Msa0830920:intron 45.0%
GGTTTAAGAGGGGAAGATAG+GGG - chr5_4:3933817-3933836 None:intergenic 45.0%
GGGTTTAAGAGGGGAAGATA+GGG - chr5_4:3933818-3933837 None:intergenic 45.0%
AGGGTTTAAGAGGGGAAGAT+AGG - chr5_4:3933819-3933838 None:intergenic 45.0%
TTGGATGGAGGGTTTAAGAG+GGG - chr5_4:3933827-3933846 None:intergenic 45.0%
GATTGGATGGAGGGTTTAAG+AGG - chr5_4:3933829-3933848 None:intergenic 45.0%
TTAGGGTGTGATTGGATGGA+GGG - chr5_4:3933838-3933857 None:intergenic 45.0%
! TTTAGGGTGTGATTGGATGG+AGG - chr5_4:3933839-3933858 None:intergenic 45.0%
!!! CCTTTTAGGGTGTGATTGGA+TGG - chr5_4:3933842-3933861 None:intergenic 45.0%
CCATCCAATCACACCCTAAA+AGG + chr5_4:3933839-3933858 Msa0830920:intron 45.0%
GCCATTATCTTCCTCTGGAT+AGG + chr5_4:3934026-3934045 Msa0830920:five_prime_UTR 45.0%
!! GCATTGTTTTCTGGTGGGAA+AGG + chr5_4:3934168-3934187 Msa0830920:five_prime_UTR 45.0%
!! CTTGGTGTTCTTCAAGTCCT+CGG - chr5_4:3934261-3934280 None:intergenic 45.0%
GAGGACTTGAAGAACACCAA+GGG + chr5_4:3934260-3934279 Msa0830920:five_prime_UTR 45.0%
TTCAACATGAAAGCAGCCCT+TGG - chr5_4:3934279-3934298 None:intergenic 45.0%
TTTCCCGACTACCACTACTA+GGG - chr5_4:3934332-3934351 None:intergenic 45.0%
! TTTTCCCGACTACCACTACT+AGG - chr5_4:3934333-3934352 None:intergenic 45.0%
TAGTAGTGGTAGTCGGGAAA+AGG + chr5_4:3934332-3934351 Msa0830920:CDS 45.0%
CAAAGGAAGCTACTTGACCT+CGG + chr5_4:3934864-3934883 Msa0830920:intron 45.0%
TTGGGCAGTTAAGTACGGTT+CGG - chr5_4:3935046-3935065 None:intergenic 45.0%
TCAATAGGTGGAGATTCTCC+TGG - chr5_4:3935168-3935187 None:intergenic 45.0%
!! TCCTCGCTTTGTTGTTCTTC+AGG + chr5_4:3935212-3935231 Msa0830920:CDS 45.0%
!! CCTCGCTTTGTTGTTCTTCA+GGG + chr5_4:3935213-3935232 Msa0830920:CDS 45.0%
! CTCGCGTTTTCGTAAGCATA+AGG - chr5_4:3935469-3935488 None:intergenic 45.0%
!! GATAGAAGTGTTGAGTCGTG+AGG - chr5_4:3935634-3935653 None:intergenic 45.0%
!! CTCGAGCAGTTCTAAGATAC+AGG + chr5_4:3935756-3935775 Msa0830920:CDS 45.0%
GTGGTTATCAGCTCTGCAAT+CGG + chr5_4:3935941-3935960 Msa0830920:CDS 45.0%
TCTGATGCATCAACTCCCAA+TGG + chr5_4:3935978-3935997 Msa0830920:CDS 45.0%
GGTCACTTCTGACTTATTCC+AGG + chr5_4:3936153-3936172 Msa0830920:three_prime_UTR 45.0%
CTTCTCTTCTTAACACGGCC+AGG + chr5_4:3933177-3933196 Msa0830920:five_prime_UTR 50.0%
TAGGTGGGGGAAAATCGGTA+TGG + chr5_4:3933433-3933452 Msa0830920:intron 50.0%
TAAGAGGGGAAGATAGGGGA+GGG - chr5_4:3933813-3933832 None:intergenic 50.0%
TTAAGAGGGGAAGATAGGGG+AGG - chr5_4:3933814-3933833 None:intergenic 50.0%
GGAAATGCCAACCTATCCAG+AGG - chr5_4:3934040-3934059 None:intergenic 50.0%
GTGTTCTTCAAGTCCTCGGA+TGG - chr5_4:3934257-3934276 None:intergenic 50.0%
CGAGGACTTGAAGAACACCA+AGG + chr5_4:3934259-3934278 Msa0830920:five_prime_UTR 50.0%
AGTATTCCCCAGATAGGGGA+GGG - chr5_4:3934921-3934940 None:intergenic 50.0%
AAGTATTCCCCAGATAGGGG+AGG - chr5_4:3934922-3934941 None:intergenic 50.0%
CCCTGAAGAACAACAAAGCG+AGG - chr5_4:3935216-3935235 None:intergenic 50.0%
TCTGCAATCGGACTGCAAAC+TGG + chr5_4:3935953-3935972 Msa0830920:CDS 50.0%
TTACACTTCGAGGGCTGCCA+TGG + chr5_4:3934304-3934323 Msa0830920:CDS 55.0%
CTACCACTACTAGGGCTCCA+TGG - chr5_4:3934324-3934343 None:intergenic 55.0%
GGAGCCCTAGTAGTGGTAGT+CGG + chr5_4:3934325-3934344 Msa0830920:CDS 55.0%
GAGCCCTAGTAGTGGTAGTC+GGG + chr5_4:3934326-3934345 Msa0830920:CDS 55.0%
TTATCCAGCCCTCCCCTATC+TGG + chr5_4:3934910-3934929 Msa0830920:intron 55.0%
TATCCAGCCCTCCCCTATCT+GGG + chr5_4:3934911-3934930 Msa0830920:intron 55.0%
TTGCGGTCAGCTCGTCTATC+CGG + chr5_4:3935008-3935027 Msa0830920:CDS 55.0%
CAGAGATGGACAGAGCTTCC+AGG + chr5_4:3935147-3935166 Msa0830920:intron 55.0%
CTTCTTAACACGGCCAGGCC+AGG + chr5_4:3933182-3933201 Msa0830920:five_prime_UTR 60.0%
CAAGTCCTCGGATGGCACCA+AGG - chr5_4:3934249-3934268 None:intergenic 60.0%
CTGCCATGGAGCCCTAGTAG+TGG + chr5_4:3934318-3934337 Msa0830920:CDS 60.0%
ATCCAGCCCTCCCCTATCTG+GGG + chr5_4:3934912-3934931 Msa0830920:intron 60.0%
!! TTGGGCCTTGGTGCCATCCG+AGG + chr5_4:3934241-3934260 Msa0830920:five_prime_UTR 65.0%
TTCCCCAGATAGGGGAGGGC+TGG - chr5_4:3934917-3934936 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 3933085 3936276 3933085 ID=Msa0830920;Name=Msa0830920
chr5_4 mRNA 3933085 3936276 3933085 ID=Msa0830920-mRNA-1;Parent=Msa0830920;Name=Msa0830920-mRNA-1;_AED=0.12;_eAED=0.12;_QI=408|0.83|0.71|1|0.83|0.71|7|266|219
chr5_4 exon 3933085 3933203 3933085 ID=Msa0830920-mRNA-1:exon:1542;Parent=Msa0830920-mRNA-1
chr5_4 exon 3934002 3934353 3934002 ID=Msa0830920-mRNA-1:exon:1543;Parent=Msa0830920-mRNA-1
chr5_4 exon 3934442 3934524 3934442 ID=Msa0830920-mRNA-1:exon:1544;Parent=Msa0830920-mRNA-1
chr5_4 exon 3934986 3935049 3934986 ID=Msa0830920-mRNA-1:exon:1545;Parent=Msa0830920-mRNA-1
chr5_4 exon 3935150 3935353 3935150 ID=Msa0830920-mRNA-1:exon:1546;Parent=Msa0830920-mRNA-1
chr5_4 exon 3935610 3935777 3935610 ID=Msa0830920-mRNA-1:exon:1547;Parent=Msa0830920-mRNA-1
chr5_4 exon 3935933 3936276 3935933 ID=Msa0830920-mRNA-1:exon:1548;Parent=Msa0830920-mRNA-1
chr5_4 five_prime_UTR 3933085 3933203 3933085 ID=Msa0830920-mRNA-1:five_prime_utr;Parent=Msa0830920-mRNA-1
chr5_4 five_prime_UTR 3934002 3934290 3934002 ID=Msa0830920-mRNA-1:five_prime_utr;Parent=Msa0830920-mRNA-1
chr5_4 CDS 3934291 3934353 3934291 ID=Msa0830920-mRNA-1:cds;Parent=Msa0830920-mRNA-1
chr5_4 CDS 3934442 3934524 3934442 ID=Msa0830920-mRNA-1:cds;Parent=Msa0830920-mRNA-1
chr5_4 CDS 3934986 3935049 3934986 ID=Msa0830920-mRNA-1:cds;Parent=Msa0830920-mRNA-1
chr5_4 CDS 3935150 3935353 3935150 ID=Msa0830920-mRNA-1:cds;Parent=Msa0830920-mRNA-1
chr5_4 CDS 3935610 3935777 3935610 ID=Msa0830920-mRNA-1:cds;Parent=Msa0830920-mRNA-1
chr5_4 CDS 3935933 3936010 3935933 ID=Msa0830920-mRNA-1:cds;Parent=Msa0830920-mRNA-1
chr5_4 three_prime_UTR 3936011 3936276 3936011 ID=Msa0830920-mRNA-1:three_prime_utr;Parent=Msa0830920-mRNA-1
Gene Sequence

>Msa0830920

ATGTTGAAATTGTTTACACTTCGAGGGCTGCCATGGAGCCCTAGTAGTGGTAGTCGGGAAAAGGTTGAACTAACAGCTGCAGAAGTAGAGTCTCTTCGATCAGAACTTGTTGATTTAGAGGAGAAGGAGGCTCAGTTAAAAGCTCAGTTGGAAAATATTGATGAAATTTTGCGGTCAGCTCGTCTATCCGGATATTTATACATCCGAACCAGATGGACAGAGCTTCCAGGAGAATCTCCACCTATTGATGATACCGATGTAGATGATTGGCTTCCTCGCTTTGTTGTTCTTCAGGGTGAATGTCTCTTCATATATTTGTTGAGTACAGGTAGTGCTACTAACTTCTATTTTATATGCAGAGCTGTATTTCTCACTTTAAGGCCTTTACCCGATGAATCTCTCTTATATTCTCTCGGGGAAATTGCAGACTTAAGTCCTCACGACTCAACACTTCTATCTGACATTGTTGAAGTATACCAATTACCAAGTATTAAGCGTGAAGATGGTGAGATGAGATACGCCTTTTGTATTTTAACACGACATGGATTACGATATGAATGCTCGAGCAGTTCTAAGATACAGGTGGATTCGTGGTTATCAGCTCTGCAATCGGACTGCAAACTGGAATCTGATGCATCAACTCCCAATGGTTCGATATAG
Protein sequence

>Msa0830920

MLKLFTLRGLPWSPSSGSREKVELTAAEVESLRSELVDLEEKEAQLKAQLENIDEILRSARLSGYLYIRTRWTELPGESPPIDDTDVDDWLPRFVVLQGECLFIYLLSTGSATNFYFICRAVFLTLRPLPDESLLYSLGEIADLSPHDSTLLSDIVEVYQLPSIKREDGEMRYAFCILTRHGLRYECSSSSKIQVDSWLSALQSDCKLESDASTPNGSI*