AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0712250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712250 MtrunA17_Chr5g0396661 81.735 219 7 1 1 219 1 186 1.06e-121 342
Msa0712250 MtrunA17_Chr8g0388141 63.470 219 47 1 1 219 62 247 1.47e-90 266
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712250 AT4G23895.4 60.274 219 52 2 1 219 60 243 1.46e-82 246
Msa0712250 AT4G23895.2 60.274 219 52 2 1 219 60 243 1.46e-82 246
Msa0712250 AT4G23895.1 60.274 219 52 2 1 219 60 243 1.46e-82 246
Msa0712250 AT4G23895.3 61.722 209 45 2 1 209 60 233 3.76e-78 242

Find 72 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCTGTATTTCTCACTTTA+AGG 0.099261 5_1:+4074762 Msa0712250:CDS
TCGCACAATCTAGCTTATTT+TGG 0.119039 5_1:+4073686 Msa0712250:five_prime_UTR
TCCTCTAATTCAAAATTTAA+TGG 0.168766 5_1:-4075653 None:intergenic
CACGCTTAATACTTGGTAAT+TGG 0.189394 5_1:-4075136 None:intergenic
TCCTCGCTTTGTTGTTCTTC+AGG 0.207772 5_1:+4074676 Msa0712250:CDS
ACTAATGAACTTTGCTAGTT+TGG 0.230127 5_1:+4075676 Msa0712250:three_prime_UTR
AATAGAGACTGATGATTATT+TGG 0.240870 5_1:+4075598 Msa0712250:three_prime_UTR
TGAGTTCCAGATTCAGTAAT+AGG 0.270097 5_1:-4073597 None:intergenic
ATCAGAACTTGTTGATTTAG+AGG 0.291898 5_1:+4073941 Msa0712250:CDS
CCTCGCTTTGTTGTTCTTCA+GGG 0.314121 5_1:+4074677 Msa0712250:CDS
CCTCTAATTCAAAATTTAAT+GGG 0.325775 5_1:-4075652 None:intergenic
GGCATTTCCTTGAGAACTTA+AGG 0.331835 5_1:+4073515 Msa0712250:five_prime_UTR
AAGGCACCTATTACTGAATC+TGG 0.344705 5_1:+4073591 Msa0712250:five_prime_UTR
GATGTTTCAGGATTATTTAT+CGG 0.365961 5_1:+4073456 Msa0712250:intron
GGTCACTTCTGACTTATTCC+AGG 0.382654 5_1:+4075619 Msa0712250:three_prime_UTR
CCATCTTCACGCTTAATACT+TGG 0.382946 5_1:-4075143 None:intergenic
TATAACTAACTTAACTGTTC+AGG 0.384599 5_1:-4075732 None:intergenic
TTGCGGTCAGCTCGTCTATC+CGG 0.396043 5_1:+4074472 Msa0712250:CDS
TTGAAATTGTTTACACTTCG+AGG 0.404814 5_1:+4073758 Msa0712250:CDS
CAGAGATGGACAGAGCTTCC+AGG 0.406603 5_1:+4074611 Msa0712250:intron
CTCGAGCAGTTCTAAGATAC+AGG 0.424024 5_1:+4075220 Msa0712250:CDS
TCTGCAATCGGACTGCAAAC+TGG 0.436293 5_1:+4075419 Msa0712250:CDS
AGTTTAAAAGCAAGTTTGTC+GGG 0.443241 5_1:+4073088 Msa0712250:five_prime_UTR
TCTACATCGGTATCATCAAT+AGG 0.447712 5_1:-4074644 None:intergenic
ACCTATCCAGAGGAAGATAA+TGG 0.451212 5_1:-4073491 None:intergenic
TTCATATATTTGTTGAGTAC+AGG 0.456033 5_1:+4074710 Msa0712250:CDS
TTATCGCCATTATCTTCCTC+TGG 0.456512 5_1:+4073485 Msa0712250:five_prime_UTR
ACTTGTTGATTTAGAGGAGA+AGG 0.457112 5_1:+4073947 Msa0712250:CDS
TTAATCAAAATCAATAGTAC+TGG 0.466308 5_1:-4073034 None:intergenic
TTATCTTCCTCTGGATAGGT+TGG 0.480663 5_1:+4073494 Msa0712250:five_prime_UTR
ATGTCGTGTTAAAATACAAA+AGG 0.492182 5_1:-4075180 None:intergenic
GGTTCGGATGTATAAATATC+CGG 0.494584 5_1:-4074491 None:intergenic
AGAATATAAGAGAGATTCAT+CGG 0.494851 5_1:-4074792 None:intergenic
CTTGGTGTTCTTCAAGTCCT+CGG 0.496975 5_1:-4073722 None:intergenic
TAGTTTAAAAGCAAGTTTGT+CGG 0.497053 5_1:+4073087 Msa0712250:five_prime_UTR
CCAAGTATTAAGCGTGAAGA+TGG 0.499854 5_1:+4075143 Msa0712250:CDS
TTACATCTATATCGAACCAT+TGG 0.502885 5_1:-4075460 None:intergenic
ATGATACCGATGTAGATGAT+TGG 0.507290 5_1:+4074651 Msa0712250:CDS
TAAGCTAGATTGTGCGATTG+AGG 0.508755 5_1:-4073680 None:intergenic
GAGCCCTAGTAGTGGTAGTC+GGG 0.510554 5_1:+4073790 Msa0712250:CDS
TAGTAGTGGTAGTCGGGAAA+AGG 0.515903 5_1:+4073796 Msa0712250:CDS
ACTGGATTTCTTTCATTCGA+AGG 0.518198 5_1:-4073016 None:intergenic
TTCAACATGAAAGCAGCCCT+TGG 0.520437 5_1:-4073740 None:intergenic
GTGTTCTTCAAGTCCTCGGA+TGG 0.524855 5_1:-4073718 None:intergenic
AAGAGAGATTCATCGGGTAA+AGG 0.527630 5_1:-4074785 None:intergenic
GTGGTTATCAGCTCTGCAAT+CGG 0.533434 5_1:+4075407 Msa0712250:CDS
TACATCTATATCGAACCATT+GGG 0.534421 5_1:-4075459 None:intergenic
TTACACTTCGAGGGCTGCCA+TGG 0.535812 5_1:+4073768 Msa0712250:CDS
CAGATGACCTTAAGTTCTCA+AGG 0.536063 5_1:-4073522 None:intergenic
TCAATAGGTGGAGATTCTCC+TGG 0.549524 5_1:-4074629 None:intergenic
TTGGGCCTTGGTGCCATCCG+AGG 0.549919 5_1:+4073705 Msa0712250:five_prime_UTR
TGAAATTGTTTACACTTCGA+GGG 0.554877 5_1:+4073759 Msa0712250:CDS
TTAATGGGTCAATCAAGTCC+TGG 0.561085 5_1:-4075637 None:intergenic
TGTACAAGAAATAATAGTCA+TGG 0.573002 5_1:-4075557 None:intergenic
GAATATAAGAGAGATTCATC+GGG 0.575538 5_1:-4074791 None:intergenic
GCCATTATCTTCCTCTGGAT+AGG 0.579320 5_1:+4073490 Msa0712250:five_prime_UTR
GGAGCCCTAGTAGTGGTAGT+CGG 0.588289 5_1:+4073789 Msa0712250:CDS
TGTTGATTTAGAGGAGAAGG+AGG 0.603598 5_1:+4073950 Msa0712250:CDS
CTACCACTACTAGGGCTCCA+TGG 0.603848 5_1:-4073785 None:intergenic
AATAAAGATCAACACTCTGA+AGG 0.618147 5_1:+4073656 Msa0712250:five_prime_UTR
TTTCCCGACTACCACTACTA+GGG 0.626466 5_1:-4073793 None:intergenic
TCTGATGCATCAACTCCCAA+TGG 0.627776 5_1:+4075444 Msa0712250:CDS
CCCTGAAGAACAACAAAGCG+AGG 0.633323 5_1:-4074677 None:intergenic
TGTAGAATATCTTGAGAACA+AGG 0.639246 5_1:+4073572 Msa0712250:five_prime_UTR
CTGCCATGGAGCCCTAGTAG+TGG 0.643912 5_1:+4073782 Msa0712250:CDS
GATAGAAGTGTTGAGTCGTG+AGG 0.659394 5_1:-4075095 None:intergenic
AGGAAGCCAATCATCTACAT+CGG 0.663664 5_1:-4074657 None:intergenic
CAAGTCCTCGGATGGCACCA+AGG 0.664072 5_1:-4073710 None:intergenic
ACATCGGTATCATCAATAGG+TGG 0.664944 5_1:-4074641 None:intergenic
GAGGACTTGAAGAACACCAA+GGG 0.673825 5_1:+4073724 Msa0712250:five_prime_UTR
GGAAATGCCAACCTATCCAG+AGG 0.683785 5_1:-4073501 None:intergenic
CGAGGACTTGAAGAACACCA+AGG 0.708009 5_1:+4073723 Msa0712250:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTTTTCTTCTTTAAATTA+CGG - chr5_1:4073252-4073271 None:intergenic 15.0%
!!! AAAATATTGATGAAATTTTG+CGG + chr5_1:4074455-4074474 Msa0712250:CDS 15.0%
!! TTAATCAAAATCAATAGTAC+TGG - chr5_1:4073037-4073056 None:intergenic 20.0%
!! ATAAACTGAATATGTAGAAT+CGG - chr5_1:4073190-4073209 None:intergenic 20.0%
!!! CTACAAAATATAATTGACTT+TGG - chr5_1:4073886-4073905 None:intergenic 20.0%
!!! CAAAGTCAATTATATTTTGT+AGG + chr5_1:4073884-4073903 Msa0712250:intron 20.0%
!!! TTGATTTTTTTATTTGACAC+AGG + chr5_1:4074428-4074447 Msa0712250:intron 20.0%
!! AAATAAACTAATGAAGGTTT+GGG - chr5_1:4074528-4074547 None:intergenic 20.0%
!! AAAATAAACTAATGAAGGTT+TGG - chr5_1:4074529-4074548 None:intergenic 20.0%
!! ATAAGGATAAAAAAATAGAG+AGG - chr5_1:4074916-4074935 None:intergenic 20.0%
!!! CTCTTTTTACCAATATTTTT+CGG + chr5_1:4075018-4075037 Msa0712250:intron 20.0%
!! TAATCAGATATTCTTAAGTT+AGG - chr5_1:4075250-4075269 None:intergenic 20.0%
!! CCTCTAATTCAAAATTTAAT+GGG - chr5_1:4075655-4075674 None:intergenic 20.0%
!! TCCTCTAATTCAAAATTTAA+TGG - chr5_1:4075656-4075675 None:intergenic 20.0%
! TAGTTTAAAAGCAAGTTTGT+CGG + chr5_1:4073087-4073106 Msa0712250:five_prime_UTR 25.0%
!! AAAGTCCAAAAATCCTTTTA+GGG - chr5_1:4073319-4073338 None:intergenic 25.0%
!! TAAAGTCCAAAAATCCTTTT+AGG - chr5_1:4073320-4073339 None:intergenic 25.0%
!! GACTTTATATTTTGCAAGAT+TGG + chr5_1:4073333-4073352 Msa0712250:intron 25.0%
! AATATGGTAGTACTTAGATA+CGG + chr5_1:4073361-4073380 Msa0712250:intron 25.0%
! GATGTTTCAGGATTATTTAT+CGG + chr5_1:4073456-4073475 Msa0712250:intron 25.0%
! TGAAGAACTACAAAACAATA+AGG - chr5_1:4074274-4074293 None:intergenic 25.0%
! AGCAAAAAAGAATCACTATA+TGG - chr5_1:4074304-4074323 None:intergenic 25.0%
!!! ATTCTTTTTTGCTGTTACAA+AGG + chr5_1:4074311-4074330 Msa0712250:intron 25.0%
!!! TTTTTTTATTTGACACAGGT+TGG + chr5_1:4074432-4074451 Msa0712250:intron 25.0%
! GATGCAAAATAAACTAATGA+AGG - chr5_1:4074534-4074553 None:intergenic 25.0%
!!! TCATTTTTTATCTACAGAGA+TGG + chr5_1:4074597-4074616 Msa0712250:intron 25.0%
!! TTCATATATTTGTTGAGTAC+AGG + chr5_1:4074710-4074729 Msa0712250:CDS 25.0%
! AGAATATAAGAGAGATTCAT+CGG - chr5_1:4074795-4074814 None:intergenic 25.0%
! CGAAAAATATTGGTAAAAAG+AGG - chr5_1:4075020-4075039 None:intergenic 25.0%
! ATGTCGTGTTAAAATACAAA+AGG - chr5_1:4075183-4075202 None:intergenic 25.0%
!!! TTTTGTATTTTAACACGACA+TGG + chr5_1:4075182-4075201 Msa0712250:CDS 25.0%
!!! TCTTTTATTCCTTTTTAAGG+TGG + chr5_1:4075380-4075399 Msa0712250:intron 25.0%
! TGTACAAGAAATAATAGTCA+TGG - chr5_1:4075560-4075579 None:intergenic 25.0%
! AATAGAGACTGATGATTATT+TGG + chr5_1:4075598-4075617 Msa0712250:three_prime_UTR 25.0%
!! CCCATTAAATTTTGAATTAG+AGG + chr5_1:4075652-4075671 Msa0712250:three_prime_UTR 25.0%
AGTTTAAAAGCAAGTTTGTC+GGG + chr5_1:4073088-4073107 Msa0712250:five_prime_UTR 30.0%
ATATGGTAGTACTTAGATAC+GGG + chr5_1:4073362-4073381 Msa0712250:intron 30.0%
TGATATTACTATTGGCAACT+TGG - chr5_1:4073396-4073415 None:intergenic 30.0%
TCCTCTAGTGATATTACTAT+TGG - chr5_1:4073404-4073423 None:intergenic 30.0%
TGTAGAATATCTTGAGAACA+AGG + chr5_1:4073572-4073591 Msa0712250:five_prime_UTR 30.0%
! CTGGAACTCATAAATCTTTT+AGG + chr5_1:4073610-4073629 Msa0712250:five_prime_UTR 30.0%
!! ATCTTTTAGGCATTGTTTTC+TGG + chr5_1:4073623-4073642 Msa0712250:five_prime_UTR 30.0%
AATAAAGATCAACACTCTGA+AGG + chr5_1:4073656-4073675 Msa0712250:five_prime_UTR 30.0%
TTGAAATTGTTTACACTTCG+AGG + chr5_1:4073758-4073777 Msa0712250:CDS 30.0%
TGAAATTGTTTACACTTCGA+GGG + chr5_1:4073759-4073778 Msa0712250:CDS 30.0%
! GTTTCTTTTGTCATTGATGA+AGG + chr5_1:4073845-4073864 Msa0712250:intron 30.0%
!! ATCAGAACTTGTTGATTTAG+AGG + chr5_1:4073941-4073960 Msa0712250:CDS 30.0%
GCTGTAACAGTAAAAAGTTA+TGG - chr5_1:4074001-4074020 None:intergenic 30.0%
AAGTGATTGTCTTAGTAGTA+GGG + chr5_1:4074229-4074248 Msa0712250:intron 30.0%
GAATATAAGAGAGATTCATC+GGG - chr5_1:4074794-4074813 None:intergenic 30.0%
AATAGAGAGGAGAGAAAAAA+AGG - chr5_1:4074903-4074922 None:intergenic 30.0%
!! ACCAATATTTTTCGGATGAT+TGG + chr5_1:4075026-4075045 Msa0712250:intron 30.0%
TCATTTACAATTGCTGATGA+AGG + chr5_1:4075052-4075071 Msa0712250:intron 30.0%
CATTTACAATTGCTGATGAA+GGG + chr5_1:4075053-4075072 Msa0712250:intron 30.0%
ATTTACAATTGCTGATGAAG+GGG + chr5_1:4075054-4075073 Msa0712250:intron 30.0%
! CGCTCTTTTATTCCTTTTTA+AGG + chr5_1:4075377-4075396 Msa0712250:intron 30.0%
TACATCTATATCGAACCATT+GGG - chr5_1:4075462-4075481 None:intergenic 30.0%
TTACATCTATATCGAACCAT+TGG - chr5_1:4075463-4075482 None:intergenic 30.0%
! AACATGTTTTACTCCTTGTT+TGG + chr5_1:4075481-4075500 Msa0712250:three_prime_UTR 30.0%
!! ACATGTTTTACTCCTTGTTT+GGG + chr5_1:4075482-4075501 Msa0712250:three_prime_UTR 30.0%
ACTAATGAACTTTGCTAGTT+TGG + chr5_1:4075676-4075695 Msa0712250:three_prime_UTR 30.0%
ACTGGATTTCTTTCATTCGA+AGG - chr5_1:4073019-4073038 None:intergenic 35.0%
GCTATTAGTAGAAGAAGTGA+TGG + chr5_1:4073139-4073158 Msa0712250:intron 35.0%
!!! AAATCCTTTTAGGGTGTGAT+TGG - chr5_1:4073310-4073329 None:intergenic 35.0%
! TCACACCCTAAAAGGATTTT+TGG + chr5_1:4073311-4073330 Msa0712250:intron 35.0%
!! TGCAAGATTGGTTTGCAATA+TGG + chr5_1:4073345-4073364 Msa0712250:intron 35.0%
GCCAATAGTAATATCACTAG+AGG + chr5_1:4073400-4073419 Msa0712250:intron 35.0%
ACATCAACAATGCTAGAAGT+TGG - chr5_1:4073441-4073460 None:intergenic 35.0%
TCTAGCATTGTTGATGTTTC+AGG + chr5_1:4073444-4073463 Msa0712250:intron 35.0%
!! CACTCTCAAGTTTTAACATC+TGG + chr5_1:4073549-4073568 Msa0712250:five_prime_UTR 35.0%
TGAGTTCCAGATTCAGTAAT+AGG - chr5_1:4073600-4073619 None:intergenic 35.0%
!! TTTTAGGCATTGTTTTCTGG+TGG + chr5_1:4073626-4073645 Msa0712250:five_prime_UTR 35.0%
!! TTTAGGCATTGTTTTCTGGT+GGG + chr5_1:4073627-4073646 Msa0712250:five_prime_UTR 35.0%
!! TCGCACAATCTAGCTTATTT+TGG + chr5_1:4073686-4073705 Msa0712250:five_prime_UTR 35.0%
!! CGCACAATCTAGCTTATTTT+GGG + chr5_1:4073687-4073706 Msa0712250:five_prime_UTR 35.0%
!! ACTTGTTGATTTAGAGGAGA+AGG + chr5_1:4073947-4073966 Msa0712250:CDS 35.0%
! AGTCAAATAGCAGTTAGCTT+TGG + chr5_1:4074097-4074116 Msa0712250:intron 35.0%
!! GTCAAATAGCAGTTAGCTTT+GGG + chr5_1:4074098-4074117 Msa0712250:intron 35.0%
ACATTGAACATGACAAAGCT+AGG + chr5_1:4074169-4074188 Msa0712250:intron 35.0%
CAAGTGATTGTCTTAGTAGT+AGG + chr5_1:4074228-4074247 Msa0712250:intron 35.0%
!! AAAAAGTATTCCCCAGATAG+GGG - chr5_1:4074389-4074408 None:intergenic 35.0%
!! CAAAAAGTATTCCCCAGATA+GGG - chr5_1:4074390-4074409 None:intergenic 35.0%
!! ACAAAAAGTATTCCCCAGAT+AGG - chr5_1:4074391-4074410 None:intergenic 35.0%
GGTTCGGATGTATAAATATC+CGG - chr5_1:4074494-4074513 None:intergenic 35.0%
TCTACATCGGTATCATCAAT+AGG - chr5_1:4074647-4074666 None:intergenic 35.0%
ATGATACCGATGTAGATGAT+TGG + chr5_1:4074651-4074670 Msa0712250:CDS 35.0%
GAGCTGTATTTCTCACTTTA+AGG + chr5_1:4074762-4074781 Msa0712250:CDS 35.0%
GCCAATCATCCGAAAAATAT+TGG - chr5_1:4075030-4075049 None:intergenic 35.0%
TTTACAATTGCTGATGAAGG+GGG + chr5_1:4075055-4075074 Msa0712250:intron 35.0%
! CACGCTTAATACTTGGTAAT+TGG - chr5_1:4075139-4075158 None:intergenic 35.0%
!! CATGTTTTACTCCTTGTTTG+GGG + chr5_1:4075483-4075502 Msa0712250:three_prime_UTR 35.0%
!! ATGTTTTACTCCTTGTTTGG+GGG + chr5_1:4075484-4075503 Msa0712250:three_prime_UTR 35.0%
TCTACTAATAGCAAGCTCAC+CGG - chr5_1:4073131-4073150 None:intergenic 40.0%
ATTGGATGGAGGGTTTAAGA+GGG - chr5_1:4073292-4073311 None:intergenic 40.0%
TTATCGCCATTATCTTCCTC+TGG + chr5_1:4073485-4073504 Msa0712250:five_prime_UTR 40.0%
ACCTATCCAGAGGAAGATAA+TGG - chr5_1:4073494-4073513 None:intergenic 40.0%
TTATCTTCCTCTGGATAGGT+TGG + chr5_1:4073494-4073513 Msa0712250:five_prime_UTR 40.0%
GGCATTTCCTTGAGAACTTA+AGG + chr5_1:4073515-4073534 Msa0712250:five_prime_UTR 40.0%
CAGATGACCTTAAGTTCTCA+AGG - chr5_1:4073525-4073544 None:intergenic 40.0%
! AAGGCACCTATTACTGAATC+TGG + chr5_1:4073591-4073610 Msa0712250:five_prime_UTR 40.0%
!! CATTGTTTTCTGGTGGGAAA+GGG + chr5_1:4073633-4073652 Msa0712250:five_prime_UTR 40.0%
TAAGCTAGATTGTGCGATTG+AGG - chr5_1:4073683-4073702 None:intergenic 40.0%
!!! ATCTAGCTTATTTTGGGCCT+TGG + chr5_1:4073693-4073712 Msa0712250:five_prime_UTR 40.0%
! GGAAAAGGTTTGCTTTCATC+AGG + chr5_1:4073811-4073830 Msa0712250:intron 40.0%
!! TGTTGATTTAGAGGAGAAGG+AGG + chr5_1:4073950-4073969 Msa0712250:CDS 40.0%
CATGACAAAGCTAGGATTTC+AGG + chr5_1:4074177-4074196 Msa0712250:intron 40.0%
! TCAGGTTCTAGCCATAGATT+TGG + chr5_1:4074195-4074214 Msa0712250:intron 40.0%
GTGACTAGACTCCAAATCTA+TGG - chr5_1:4074209-4074228 None:intergenic 40.0%
AACACTGAATTTCAACTCCG+AGG - chr5_1:4074348-4074367 None:intergenic 40.0%
AAGGTTTGGGCAGTTAAGTA+CGG - chr5_1:4074515-4074534 None:intergenic 40.0%
GTTAGGTAATGACAAGCAGA+TGG - chr5_1:4074556-4074575 None:intergenic 40.0%
CAATCAGTACTAAGCGTGTT+AGG - chr5_1:4074573-4074592 None:intergenic 40.0%
!! CTAACACGCTTAGTACTGAT+TGG + chr5_1:4074571-4074590 Msa0712250:intron 40.0%
ACATCGGTATCATCAATAGG+TGG - chr5_1:4074644-4074663 None:intergenic 40.0%
AGGAAGCCAATCATCTACAT+CGG - chr5_1:4074660-4074679 None:intergenic 40.0%
AAGAGAGATTCATCGGGTAA+AGG - chr5_1:4074788-4074807 None:intergenic 40.0%
CCATCTTCACGCTTAATACT+TGG - chr5_1:4075146-4075165 None:intergenic 40.0%
CCAAGTATTAAGCGTGAAGA+TGG + chr5_1:4075143-4075162 Msa0712250:CDS 40.0%
!!! TCCTTTTTAAGGTGGATTCG+TGG + chr5_1:4075388-4075407 Msa0712250:intron 40.0%
ACCACGAATCCACCTTAAAA+AGG - chr5_1:4075392-4075411 None:intergenic 40.0%
ATCTCAAATTCCCCCAAACA+AGG - chr5_1:4075497-4075516 None:intergenic 40.0%
TTGTTTGGGGGAATTTGAGA+TGG + chr5_1:4075496-4075515 Msa0712250:three_prime_UTR 40.0%
! TTAATGGGTCAATCAAGTCC+TGG - chr5_1:4075640-4075659 None:intergenic 40.0%
! GGACTGCTAGTCTAACAATC+CGG + chr5_1:4073109-4073128 Msa0712250:intron 45.0%
GGTTTAAGAGGGGAAGATAG+GGG - chr5_1:4073281-4073300 None:intergenic 45.0%
GGGTTTAAGAGGGGAAGATA+GGG - chr5_1:4073282-4073301 None:intergenic 45.0%
AGGGTTTAAGAGGGGAAGAT+AGG - chr5_1:4073283-4073302 None:intergenic 45.0%
TTGGATGGAGGGTTTAAGAG+GGG - chr5_1:4073291-4073310 None:intergenic 45.0%
GATTGGATGGAGGGTTTAAG+AGG - chr5_1:4073293-4073312 None:intergenic 45.0%
TTAGGGTGTGATTGGATGGA+GGG - chr5_1:4073302-4073321 None:intergenic 45.0%
! TTTAGGGTGTGATTGGATGG+AGG - chr5_1:4073303-4073322 None:intergenic 45.0%
!!! CCTTTTAGGGTGTGATTGGA+TGG - chr5_1:4073306-4073325 None:intergenic 45.0%
CCATCCAATCACACCCTAAA+AGG + chr5_1:4073303-4073322 Msa0712250:intron 45.0%
GCCATTATCTTCCTCTGGAT+AGG + chr5_1:4073490-4073509 Msa0712250:five_prime_UTR 45.0%
!! GCATTGTTTTCTGGTGGGAA+AGG + chr5_1:4073632-4073651 Msa0712250:five_prime_UTR 45.0%
!! CTTGGTGTTCTTCAAGTCCT+CGG - chr5_1:4073725-4073744 None:intergenic 45.0%
GAGGACTTGAAGAACACCAA+GGG + chr5_1:4073724-4073743 Msa0712250:five_prime_UTR 45.0%
TTCAACATGAAAGCAGCCCT+TGG - chr5_1:4073743-4073762 None:intergenic 45.0%
TTTCCCGACTACCACTACTA+GGG - chr5_1:4073796-4073815 None:intergenic 45.0%
! TTTTCCCGACTACCACTACT+AGG - chr5_1:4073797-4073816 None:intergenic 45.0%
TAGTAGTGGTAGTCGGGAAA+AGG + chr5_1:4073796-4073815 Msa0712250:CDS 45.0%
CAAAGGAAGCTACTTGACCT+CGG + chr5_1:4074328-4074347 Msa0712250:intron 45.0%
TTGGGCAGTTAAGTACGGTT+CGG - chr5_1:4074510-4074529 None:intergenic 45.0%
TCAATAGGTGGAGATTCTCC+TGG - chr5_1:4074632-4074651 None:intergenic 45.0%
!! TCCTCGCTTTGTTGTTCTTC+AGG + chr5_1:4074676-4074695 Msa0712250:CDS 45.0%
!! CCTCGCTTTGTTGTTCTTCA+GGG + chr5_1:4074677-4074696 Msa0712250:CDS 45.0%
! CTCGCGTTTTCGTAAGCATA+AGG - chr5_1:4074933-4074952 None:intergenic 45.0%
!! GATAGAAGTGTTGAGTCGTG+AGG - chr5_1:4075098-4075117 None:intergenic 45.0%
!! CTCGAGCAGTTCTAAGATAC+AGG + chr5_1:4075220-4075239 Msa0712250:CDS 45.0%
GTGGTTATCAGCTCTGCAAT+CGG + chr5_1:4075407-4075426 Msa0712250:CDS 45.0%
TCTGATGCATCAACTCCCAA+TGG + chr5_1:4075444-4075463 Msa0712250:CDS 45.0%
GGTCACTTCTGACTTATTCC+AGG + chr5_1:4075619-4075638 Msa0712250:three_prime_UTR 45.0%
TAAGAGGGGAAGATAGGGGA+GGG - chr5_1:4073277-4073296 None:intergenic 50.0%
TTAAGAGGGGAAGATAGGGG+AGG - chr5_1:4073278-4073297 None:intergenic 50.0%
GGAAATGCCAACCTATCCAG+AGG - chr5_1:4073504-4073523 None:intergenic 50.0%
GTGTTCTTCAAGTCCTCGGA+TGG - chr5_1:4073721-4073740 None:intergenic 50.0%
CGAGGACTTGAAGAACACCA+AGG + chr5_1:4073723-4073742 Msa0712250:five_prime_UTR 50.0%
AGTATTCCCCAGATAGGGGA+GGG - chr5_1:4074385-4074404 None:intergenic 50.0%
AAGTATTCCCCAGATAGGGG+AGG - chr5_1:4074386-4074405 None:intergenic 50.0%
CCCTGAAGAACAACAAAGCG+AGG - chr5_1:4074680-4074699 None:intergenic 50.0%
TCTGCAATCGGACTGCAAAC+TGG + chr5_1:4075419-4075438 Msa0712250:CDS 50.0%
TTACACTTCGAGGGCTGCCA+TGG + chr5_1:4073768-4073787 Msa0712250:CDS 55.0%
CTACCACTACTAGGGCTCCA+TGG - chr5_1:4073788-4073807 None:intergenic 55.0%
GGAGCCCTAGTAGTGGTAGT+CGG + chr5_1:4073789-4073808 Msa0712250:CDS 55.0%
GAGCCCTAGTAGTGGTAGTC+GGG + chr5_1:4073790-4073809 Msa0712250:CDS 55.0%
TTATCCAGCCCTCCCCTATC+TGG + chr5_1:4074374-4074393 Msa0712250:intron 55.0%
TATCCAGCCCTCCCCTATCT+GGG + chr5_1:4074375-4074394 Msa0712250:intron 55.0%
TTGCGGTCAGCTCGTCTATC+CGG + chr5_1:4074472-4074491 Msa0712250:CDS 55.0%
CAGAGATGGACAGAGCTTCC+AGG + chr5_1:4074611-4074630 Msa0712250:intron 55.0%
CAAGTCCTCGGATGGCACCA+AGG - chr5_1:4073713-4073732 None:intergenic 60.0%
CTGCCATGGAGCCCTAGTAG+TGG + chr5_1:4073782-4073801 Msa0712250:CDS 60.0%
ATCCAGCCCTCCCCTATCTG+GGG + chr5_1:4074376-4074395 Msa0712250:intron 60.0%
!! TTGGGCCTTGGTGCCATCCG+AGG + chr5_1:4073705-4073724 Msa0712250:five_prime_UTR 65.0%
TTCCCCAGATAGGGGAGGGC+TGG - chr5_1:4074381-4074400 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 4072989 4075742 4072989 ID=Msa0712250;Name=Msa0712250
chr5_1 mRNA 4072989 4075742 4072989 ID=Msa0712250-mRNA-1;Parent=Msa0712250;Name=Msa0712250-mRNA-1;_AED=0.11;_eAED=0.11;_QI=414|0.83|0.71|1|0.83|0.71|7|266|219
chr5_1 exon 4072989 4073113 4072989 ID=Msa0712250-mRNA-1:exon:1561;Parent=Msa0712250-mRNA-1
chr5_1 exon 4073466 4073817 4073466 ID=Msa0712250-mRNA-1:exon:1562;Parent=Msa0712250-mRNA-1
chr5_1 exon 4073906 4073988 4073906 ID=Msa0712250-mRNA-1:exon:1563;Parent=Msa0712250-mRNA-1
chr5_1 exon 4074450 4074513 4074450 ID=Msa0712250-mRNA-1:exon:1564;Parent=Msa0712250-mRNA-1
chr5_1 exon 4074614 4074817 4074614 ID=Msa0712250-mRNA-1:exon:1565;Parent=Msa0712250-mRNA-1
chr5_1 exon 4075074 4075241 4075074 ID=Msa0712250-mRNA-1:exon:1566;Parent=Msa0712250-mRNA-1
chr5_1 exon 4075399 4075742 4075399 ID=Msa0712250-mRNA-1:exon:1567;Parent=Msa0712250-mRNA-1
chr5_1 five_prime_UTR 4072989 4073113 4072989 ID=Msa0712250-mRNA-1:five_prime_utr;Parent=Msa0712250-mRNA-1
chr5_1 five_prime_UTR 4073466 4073754 4073466 ID=Msa0712250-mRNA-1:five_prime_utr;Parent=Msa0712250-mRNA-1
chr5_1 CDS 4073755 4073817 4073755 ID=Msa0712250-mRNA-1:cds;Parent=Msa0712250-mRNA-1
chr5_1 CDS 4073906 4073988 4073906 ID=Msa0712250-mRNA-1:cds;Parent=Msa0712250-mRNA-1
chr5_1 CDS 4074450 4074513 4074450 ID=Msa0712250-mRNA-1:cds;Parent=Msa0712250-mRNA-1
chr5_1 CDS 4074614 4074817 4074614 ID=Msa0712250-mRNA-1:cds;Parent=Msa0712250-mRNA-1
chr5_1 CDS 4075074 4075241 4075074 ID=Msa0712250-mRNA-1:cds;Parent=Msa0712250-mRNA-1
chr5_1 CDS 4075399 4075476 4075399 ID=Msa0712250-mRNA-1:cds;Parent=Msa0712250-mRNA-1
chr5_1 three_prime_UTR 4075477 4075742 4075477 ID=Msa0712250-mRNA-1:three_prime_utr;Parent=Msa0712250-mRNA-1
Gene Sequence

>Msa0712250

ATGTTGAAATTGTTTACACTTCGAGGGCTGCCATGGAGCCCTAGTAGTGGTAGTCGGGAAAAGGTTGAACTAACAGCTGCAGAAGTAGAGTCTCTTCGATCAGAACTTGTTGATTTAGAGGAGAAGGAGGCTCAGTTAAAAGCTCAGTTGGAAAATATTGATGAAATTTTGCGGTCAGCTCGTCTATCCGGATATTTATACATCCGAACCAGATGGACAGAGCTTCCAGGAGAATCTCCACCTATTGATGATACCGATGTAGATGATTGGCTTCCTCGCTTTGTTGTTCTTCAGGGTGAATGTCTCTTCATATATTTGTTGAGTACAGGTAGTGCTACTAACTTCTATTTTATATGCAGAGCTGTATTTCTCACTTTAAGGCCTTTACCCGATGAATCTCTCTTATATTCTCTCGGGGAAATTGCAGACTTAAGTCCTCACGACTCAACACTTCTATCTGACATTGTTGAAGTATACCAATTACCAAGTATTAAGCGTGAAGATGGTGAGATGAGATACGCCTTTTGTATTTTAACACGACATGGATTACGATATGAATGCTCGAGCAGTTCTAAGATACAGGTGGATTCGTGGTTATCAGCTCTGCAATCGGACTGCAAACTGGAATCTGATGCATCAACTCCCAATGGTTCGATATAG
Protein sequence

>Msa0712250

MLKLFTLRGLPWSPSSGSREKVELTAAEVESLRSELVDLEEKEAQLKAQLENIDEILRSARLSGYLYIRTRWTELPGESPPIDDTDVDDWLPRFVVLQGECLFIYLLSTGSATNFYFICRAVFLTLRPLPDESLLYSLGEIADLSPHDSTLLSDIVEVYQLPSIKREDGEMRYAFCILTRHGLRYECSSSSKIQVDSWLSALQSDCKLESDASTPNGSI*