AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0832960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0832960 MtrunA17_Chr5g0398171 96.698 848 28 0 1 848 1 848 0.0 1723
Msa0832960 MtrunA17_Chr5g0398161 92.623 854 57 1 1 848 1 854 0.0 1665
Msa0832960 MtrunA17_Chr7g0246481 68.713 847 251 7 11 848 273 1114 0.0 1224
Msa0832960 MtrunA17_Chr8g0350951 68.123 847 253 6 15 848 207 1049 0.0 1214
Msa0832960 MtrunA17_Chr8g0380631 52.844 844 358 9 36 847 13 848 0.0 875
Msa0832960 MtrunA17_Chr5g0406161 50.174 861 360 12 38 841 16 864 0.0 870
Msa0832960 MtrunA17_Chr3g0143471 50.745 873 374 13 23 847 1 865 0.0 870
Msa0832960 MtrunA17_Chr2g0333021 45.189 821 404 16 36 837 4 797 0.0 710
Msa0832960 MtrunA17_Chr4g0003411 45.232 818 403 16 38 837 6 796 0.0 710
Msa0832960 MtrunA17_Chr3g0137821 44.418 833 407 20 37 838 4 811 0.0 672
Msa0832960 MtrunA17_Chr3g0137791 42.806 834 423 19 37 838 4 815 0.0 657
Msa0832960 MtrunA17_Chr2g0330431 40.939 767 382 20 84 837 36 744 6.33e-178 531
Msa0832960 MtrunA17_Chr8g0341931 39.042 835 396 26 37 847 7 752 3.00e-169 509
Msa0832960 MtrunA17_Chr1g0192401 31.522 276 137 11 498 765 711 942 3.32e-23 106
Msa0832960 MtrunA17_Chr1g0192401 29.148 223 114 7 249 455 365 559 2.28e-18 90.5
Msa0832960 MtrunA17_Chr5g0406151 59.677 62 25 0 359 420 17 78 4.99e-21 88.2
Msa0832960 MtrunA17_Chr2g0295411 30.455 220 110 7 252 455 394 586 3.11e-19 93.6
Msa0832960 MtrunA17_Chr2g0295411 33.838 198 100 8 540 732 785 956 3.86e-17 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0832960 AT2G42010.1 69.643 840 243 7 15 848 250 1083 0.0 1251
Msa0832960 AT2G42010.2 67.630 865 243 8 15 848 250 1108 0.0 1238
Msa0832960 AT4G11850.1 67.580 839 258 6 21 848 23 858 0.0 1204
Msa0832960 AT4G00240.1 66.150 839 274 5 15 848 94 927 0.0 1202
Msa0832960 AT4G11840.1 65.052 867 278 9 3 848 4 866 0.0 1178
Msa0832960 AT4G00240.2 64.994 837 252 6 15 848 94 892 0.0 1162
Msa0832960 AT4G00240.3 68.394 772 238 3 80 848 7 775 0.0 1154
Msa0832960 AT4G11830.2 64.836 856 277 9 13 848 5 856 0.0 1152
Msa0832960 AT4G11840.2 63.699 865 263 9 3 848 4 836 0.0 1143
Msa0832960 AT4G11830.1 63.765 850 264 9 13 848 5 824 0.0 1121
Msa0832960 AT4G35790.2 51.336 861 358 14 35 847 9 856 0.0 871
Msa0832960 AT4G35790.1 50.688 872 358 15 35 847 9 867 0.0 866
Msa0832960 AT4G11830.4 64.196 634 210 7 13 633 5 634 0.0 846
Msa0832960 AT4G11830.3 64.830 617 200 7 13 616 5 617 0.0 830
Msa0832960 AT3G15730.1 45.110 818 410 15 37 838 5 799 0.0 718
Msa0832960 AT1G52570.1 44.798 817 414 15 37 838 5 799 0.0 711
Msa0832960 AT1G52570.3 44.798 817 414 15 37 838 5 799 0.0 711
Msa0832960 AT1G52570.2 44.798 817 414 15 37 838 5 799 0.0 711
Msa0832960 AT4G35790.3 50.000 688 291 12 35 675 9 690 0.0 674
Msa0832960 AT5G25370.1 44.686 828 408 17 36 837 5 808 0.0 665
Msa0832960 AT5G25370.3 44.686 828 408 17 36 837 5 808 0.0 665
Msa0832960 AT5G25370.2 44.686 828 408 17 36 837 5 808 0.0 665
Msa0832960 AT5G25370.4 46.524 748 366 13 105 837 5 733 0.0 651
Msa0832960 AT1G55180.2 39.018 774 388 23 84 837 19 728 3.18e-167 503
Msa0832960 AT1G55180.1 38.293 820 407 26 38 837 10 750 4.44e-167 504
Msa0832960 AT3G16785.6 33.659 205 97 8 537 732 756 930 7.08e-19 92.8
Msa0832960 AT3G16785.6 29.237 236 117 8 256 475 390 591 3.89e-18 90.1
Msa0832960 AT3G16785.5 33.659 205 97 8 537 732 756 930 7.08e-19 92.8
Msa0832960 AT3G16785.5 29.237 236 117 8 256 475 390 591 3.89e-18 90.1
Msa0832960 AT3G16785.3 33.659 205 97 8 537 732 756 930 7.46e-19 92.8
Msa0832960 AT3G16785.3 29.237 236 117 8 256 475 390 591 3.93e-18 90.1
Msa0832960 AT3G16785.2 33.659 205 97 8 537 732 756 930 7.46e-19 92.8
Msa0832960 AT3G16785.2 29.237 236 117 8 256 475 390 591 3.93e-18 90.1
Msa0832960 AT3G16785.1 33.659 205 97 8 537 732 756 930 8.48e-19 92.4
Msa0832960 AT3G16785.1 29.237 236 117 8 256 475 390 591 4.53e-18 90.1
Msa0832960 AT3G16785.4 33.659 205 97 8 537 732 756 930 1.02e-18 92.4
Msa0832960 AT3G16785.4 29.237 236 117 8 256 475 390 591 3.55e-18 90.5
Msa0832960 AT3G05630.1 31.818 242 115 10 540 765 714 921 5.86e-18 89.7
Msa0832960 AT3G05630.1 28.000 250 122 6 229 455 358 572 4.01e-17 87.0

Find 176 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGGCTCATCATATAAT+TGG 0.123581 5_4:-6133124 Msa0832960:CDS
AACATGGCTCATTTGGTTTA+TGG 0.146616 5_4:-6136087 Msa0832960:CDS
GCCATGAGGTTTAGATTTCT+TGG 0.202085 5_4:+6132094 None:intergenic
GACAGAATCTCTGACATTAT+TGG 0.203366 5_4:-6133573 Msa0832960:CDS
TTCCCCATTCTTAATACTAA+TGG 0.230802 5_4:-6135613 Msa0832960:CDS
TGATGTTGGTGGAAACATTA+AGG 0.267482 5_4:-6131835 Msa0832960:CDS
ATAGCCTCTTTCGGTTCTTT+TGG 0.270707 5_4:+6133361 None:intergenic
TGGAAATCCTTTCACAGAAT+TGG 0.280674 5_4:+6133381 None:intergenic
TCTCACACCTTCTTGTGATT+TGG 0.281020 5_4:+6135213 None:intergenic
TAGTGCCTTCCATTGATCTT+TGG 0.281191 5_4:+6132168 None:intergenic
TATGGTGAAACTCCATCTTT+TGG 0.282104 5_4:-6136069 Msa0832960:CDS
CACCAACACCATTGTTATAA+AGG 0.284689 5_4:+6135524 None:intergenic
CTTCCTAGTTTGCGGGTTGA+TGG 0.291314 5_4:-6135409 Msa0832960:CDS
CAACCAACGCTCCTCAAAAT+TGG 0.295875 5_4:+6133662 None:intergenic
GGATGATGAATACGTGTTAA+TGG 0.315798 5_4:-6132207 Msa0832960:CDS
CTACTCAGAGGATTCTCTTT+TGG 0.327286 5_4:-6132732 Msa0832960:CDS
CCTACTTCTAAAAGCATGTT+TGG 0.328089 5_4:-6135166 Msa0832960:CDS
GATGGAAAAGATTGTACATT+AGG 0.338975 5_4:-6135250 Msa0832960:CDS
AGAAAGATTATAGCTTTCAT+TGG 0.342958 5_4:-6133990 Msa0832960:CDS
CTCCAACACATTCTCTCTTT+AGG 0.344293 5_4:-6133935 Msa0832960:CDS
CAACCACTTCAAGTTCCTTA+AGG 0.344845 5_4:-6136005 Msa0832960:CDS
GGTCATTGAGTGAGTATAAC+TGG 0.346798 5_4:-6131971 Msa0832960:CDS
ACCCTTTGTATAGCCTCTTT+CGG 0.356128 5_4:+6133352 None:intergenic
CAAGGAGACTTGGCATGTTC+AGG 0.356759 5_4:-6133517 Msa0832960:intron
TTGTGAAAAGTGTCCATGTT+AGG 0.365738 5_4:+6135928 None:intergenic
AACAATTTACAAAGCATTAG+AGG 0.366161 5_4:-6132526 Msa0832960:CDS
ACTACAATGTTAGTCTCATT+AGG 0.367770 5_4:-6135282 Msa0832960:CDS
AGGTTCTCTTCCTAGTTTGC+GGG 0.371513 5_4:-6135416 Msa0832960:CDS
TTAAAATGTTGCATCCAAAC+AGG 0.372747 5_4:+6135769 None:intergenic
GTGCTTCTTTGCCCAAGATC+TGG 0.384976 5_4:-6134936 Msa0832960:CDS
TTGAATGTGTGAGAAGAATA+AGG 0.385372 5_4:-6131992 Msa0832960:CDS
TTTCAAGCACTCTTCAGTTA+AGG 0.392408 5_4:-6134958 Msa0832960:CDS
CAAGTTGTTGACCTTGTCCA+TGG 0.394464 5_4:+6136037 None:intergenic
CATATCAGCCTAATCATACT+TGG 0.394543 5_4:-6132121 Msa0832960:CDS
GATTTGCATTCCAAAGTTGA+TGG 0.398771 5_4:-6133708 Msa0832960:CDS
ATGATATATGTTCATTCAAA+AGG 0.399907 5_4:-6132239 Msa0832960:CDS
GAACTTGAAGTGGTTGGAAA+TGG 0.401100 5_4:+6136012 None:intergenic
GTACTTGAGGGTACACCTTC+AGG 0.405376 5_4:+6132757 None:intergenic
TCTTGGCTCATCATATAATT+GGG 0.407407 5_4:-6133123 Msa0832960:CDS
GTATAACTGGAGACAATATT+TGG 0.407854 5_4:-6131958 Msa0832960:CDS
CTCACACATTCAATGCTCTC+TGG 0.408766 5_4:+6132002 None:intergenic
CAAAACGGTTTCAGTCTTGA+AGG 0.413615 5_4:+6135585 None:intergenic
TGCCCAAGATCTGGAGGAAA+AGG 0.418793 5_4:-6134927 Msa0832960:CDS
TTTCCATTAGTATTAAGAAT+GGG 0.420135 5_4:+6135610 None:intergenic
TTAATGTTTCCACCAACATC+AGG 0.420258 5_4:+6131837 None:intergenic
CATTGATCTTTGGTTTATGT+TGG 0.423427 5_4:+6132178 None:intergenic
CTACAATGTTAGTCTCATTA+GGG 0.423726 5_4:-6135281 Msa0832960:CDS
ATTGTTGGTTCACAGCTTAT+TGG 0.425858 5_4:-6135691 Msa0832960:CDS
TGGGTTGAGTTTCCAAAAGA+TGG 0.430054 5_4:+6136057 None:intergenic
TTGGTTGCCCAAGTATGATT+AGG 0.430796 5_4:+6132113 None:intergenic
GCCACAGAAACCGTGACATA+TGG 0.434554 5_4:+6135838 None:intergenic
GGATGACTAACCTCGTAGTT+AGG 0.436794 5_4:+6133876 None:intergenic
TAAAGGGCTACCTTATGAAT+AGG 0.439458 5_4:+6135541 None:intergenic
CGTTATCAGAACTTGGTTTG+TGG 0.441502 5_4:-6133239 Msa0832960:intron
AAGATTATAGCTTTCATTGG+AGG 0.443477 5_4:-6133987 Msa0832960:CDS
ACCAACACCATTGTTATAAA+GGG 0.443797 5_4:+6135525 None:intergenic
TGCAAACAATTTAATTCCAA+TGG 0.444897 5_4:-6132854 Msa0832960:CDS
ATCTTTAGAGCAATTTCCAT+TGG 0.451686 5_4:+6132838 None:intergenic
AAGACAATGCAAATGATGTA+TGG 0.452227 5_4:-6132549 Msa0832960:CDS
CTGATCAATTGCTAGGAAGC+TGG 0.453979 5_4:-6134074 Msa0832960:intron
GATGATGAATACGTGTTAAT+GGG 0.455690 5_4:-6132206 Msa0832960:CDS
GGCATTGTGAAGCCTCTTGT+TGG 0.456266 5_4:-6131873 Msa0832960:CDS
TCACAGCTTATTGGTGCAGT+TGG 0.457651 5_4:-6135682 Msa0832960:CDS
CCACTTGATGTTGATTCAAA+GGG 0.459671 5_4:-6131894 Msa0832960:CDS
TCCACTTGATGTTGATTCAA+AGG 0.463205 5_4:-6131895 Msa0832960:CDS
TGACATATGGATCACTGGTT+TGG 0.467645 5_4:+6135851 None:intergenic
TCTGATGCTTGATGTGCAAC+AGG 0.468636 5_4:+6135742 None:intergenic
TAGTATTAAGAATGGGGAAA+AGG 0.471331 5_4:+6135617 None:intergenic
TATGTCACGGTTTCTGTGGC+TGG 0.472374 5_4:-6135835 Msa0832960:CDS
GATAATGAAAATATTTCAAA+TGG 0.472444 5_4:-6132429 Msa0832960:CDS
GAAATGATGAGAACCCTGTT+TGG 0.472532 5_4:-6135783 Msa0832960:CDS
CCACCATCAACCCGCAAACT+AGG 0.478544 5_4:+6135406 None:intergenic
TCAGTATACTCCTATTCATA+AGG 0.478673 5_4:-6135551 Msa0832960:CDS
ATCGATTCCAATTCTGTGAA+AGG 0.478797 5_4:-6133388 Msa0832960:CDS
AAGGTTCTCTTCCTAGTTTG+CGG 0.479454 5_4:-6135417 Msa0832960:CDS
CTTAAGGAACTTGAAGTGGT+TGG 0.481851 5_4:+6136006 None:intergenic
CTAAGTGGAAAATATGTAGC+AGG 0.483576 5_4:+6135484 None:intergenic
GATGTTGGTGGAAACATTAA+GGG 0.486353 5_4:-6131834 Msa0832960:CDS
GGCAGCAGGACCATCAACTT+TGG 0.486946 5_4:+6133698 None:intergenic
ATGTAAGAGTAGGATCCTTA+AGG 0.489381 5_4:+6135990 None:intergenic
GGAGGACTTGATTTATGTCT+CGG 0.491165 5_4:-6133969 Msa0832960:CDS
CGTCGATTGGTCTATATCGT+TGG 0.492090 5_4:-6135316 Msa0832960:CDS
TATCTTTGATGCAATAAGTC+AGG 0.496499 5_4:-6135341 Msa0832960:CDS
CCTAGGCAATCGCGAGCTCT+CGG 0.501874 5_4:-6132451 Msa0832960:CDS
AAGCATGTTTGGATATAAAA+CGG 0.501929 5_4:-6135155 Msa0832960:intron
ACATAGCTGGCTCAAACAGC+AGG 0.503041 5_4:-6134904 Msa0832960:intron
AACTTGAAGTGGTTGGAAAT+GGG 0.507675 5_4:+6136013 None:intergenic
AAAATTGGTGAGGATGTCAT+AGG 0.508031 5_4:+6133677 None:intergenic
TACAAAGCATTAGAGGAGGC+TGG 0.508183 5_4:-6132519 Msa0832960:CDS
AATAAGTCAGGCGCGTCGAT+TGG 0.511402 5_4:-6135329 Msa0832960:CDS
CCCTTTGAATCAACATCAAG+TGG 0.513048 5_4:+6131894 None:intergenic
TGTGAAACCTTCCCTGATGT+TGG 0.513930 5_4:-6131849 Msa0832960:CDS
CTCTGAGTAGCTGTACTTGA+GGG 0.515931 5_4:+6132745 None:intergenic
TTAAGGATCCTACTCTTACA+TGG 0.518551 5_4:-6135988 Msa0832960:CDS
GTAAAAGACAGTGATATTGT+TGG 0.521060 5_4:-6135706 Msa0832960:CDS
CTCCTATGTGTTCACTCCAT+AGG 0.524544 5_4:+6132048 None:intergenic
TCCTATGTGTTCACTCCATA+GGG 0.526136 5_4:+6132049 None:intergenic
GCCCTTTATAACAATGGTGT+TGG 0.526442 5_4:-6135526 Msa0832960:CDS
ACCGAAAGAGGCTATACAAA+GGG 0.526697 5_4:-6133353 Msa0832960:intron
TCTCCTTGTTATGTTCATCA+AGG 0.528828 5_4:+6133532 None:intergenic
CTTAGAAATTTGCATTCAAG+AGG 0.529711 5_4:-6135962 Msa0832960:CDS
GATTGGTCTATATCGTTGGT+TGG 0.530259 5_4:-6135312 Msa0832960:CDS
TGGGATTCTTACAAAGACCT+TGG 0.531474 5_4:-6133104 Msa0832960:intron
GAAACCTTCCCTGATGTTGG+TGG 0.531756 5_4:-6131846 Msa0832960:CDS
GAAATCTAAACCTCATGGCC+AGG 0.532948 5_4:-6132090 Msa0832960:CDS
CAACATAAACCAAAGATCAA+TGG 0.533533 5_4:-6132177 Msa0832960:CDS
AAATCATGCCATGGTTGTCT+TGG 0.535876 5_4:+6133726 None:intergenic
AAACCTCATGGCCAGGTGCA+TGG 0.536424 5_4:-6132083 Msa0832960:CDS
TGTGATGGTGCAAAAGTTGA+AGG 0.538735 5_4:-6135640 Msa0832960:CDS
CTGAATAGATAAGCTCAAAA+CGG 0.543575 5_4:+6135570 None:intergenic
TTCCATTAGTATTAAGAATG+GGG 0.546474 5_4:+6135611 None:intergenic
GAAAAGTGTCCATGTTAGGA+AGG 0.547302 5_4:+6135932 None:intergenic
GTCATAGTGGATGCTGATGC+TGG 0.547843 5_4:-6134023 Msa0832960:CDS
CTAAGTTGCCATGTAAGAGT+AGG 0.549310 5_4:+6135980 None:intergenic
GTGAGGATGTCATAGGCAGC+AGG 0.549590 5_4:+6133684 None:intergenic
CTCATTAGGGATACTAGAGA+TGG 0.553430 5_4:-6135268 Msa0832960:CDS
GAGAGTGCTTCTACTTGTGT+GGG 0.553897 5_4:-6135194 Msa0832960:CDS
CCGAGAGCTCGCGATTGCCT+AGG 0.555324 5_4:+6132451 None:intergenic
AACCGTGACATATGGATCAC+TGG 0.555417 5_4:+6135846 None:intergenic
CTTTATAACAATGGTGTTGG+TGG 0.555512 5_4:-6135523 Msa0832960:CDS
AAGTTGTTGACCTTGTCCAT+GGG 0.555522 5_4:+6136038 None:intergenic
AGAGACACAGAAATTGCAAT+GGG 0.556833 5_4:-6132146 Msa0832960:CDS
CTGGAGGAAAAGGACATAGC+TGG 0.557282 5_4:-6134917 Msa0832960:CDS
ATTTCCAAAAGAACCGAAAG+AGG 0.558961 5_4:-6133365 Msa0832960:CDS
TCAAATGGTGTAAAATCTAA+CGG 0.559838 5_4:-6132414 Msa0832960:CDS
CCTCTGAGTAGCTGTACTTG+AGG 0.560599 5_4:+6132744 None:intergenic
TCATCAGAAGACAGTCATAG+TGG 0.563195 5_4:-6134036 Msa0832960:CDS
GATCCTTAAGGAACTTGAAG+TGG 0.566587 5_4:+6136002 None:intergenic
AAAACGGTTTCAGTCTTGAA+GGG 0.570945 5_4:+6135586 None:intergenic
TCATTCAAAAGGAATGATAG+TGG 0.571139 5_4:-6132228 Msa0832960:CDS
TGAAAATGAGTATGAACCAC+AGG 0.574704 5_4:-6132493 Msa0832960:CDS
CTCAAAGCCAAATCACAAGA+AGG 0.575595 5_4:-6135220 Msa0832960:CDS
GTGATTCCTATGTGGCCTGA+AGG 0.577680 5_4:-6132772 Msa0832960:CDS
TGAGAGTGCTTCTACTTGTG+TGG 0.580023 5_4:-6135195 Msa0832960:CDS
GTTATGAGAGTGGAAATTGT+TGG 0.584095 5_4:-6135369 Msa0832960:CDS
CTTCTTTGCCCAAGATCTGG+AGG 0.588065 5_4:-6134933 Msa0832960:CDS
GTCCTAAAGAGAGAATGTGT+TGG 0.588200 5_4:+6133933 None:intergenic
GGAAACTCAACCCATGGACA+AGG 0.589018 5_4:-6136048 Msa0832960:CDS
GGTACACCTTCAGGCCACAT+AGG 0.590249 5_4:+6132766 None:intergenic
GTTTCCACCAACATCAGGGA+AGG 0.590800 5_4:+6131842 None:intergenic
CCTAGTTTGCGGGTTGATGG+TGG 0.591417 5_4:-6135406 Msa0832960:CDS
CAAGATGCTCATGTTCCACA+AGG 0.593601 5_4:-6135436 Msa0832960:CDS
TAAAATGTTGCATCCAAACA+GGG 0.594950 5_4:+6135770 None:intergenic
ACCAAGAAATCTAAACCTCA+TGG 0.595362 5_4:-6132095 Msa0832960:CDS
ATGAACATAACAAGGAGACT+TGG 0.598531 5_4:-6133527 Msa0832960:CDS
AAGGTTTCACAGCCAACAAG+AGG 0.599819 5_4:+6131861 None:intergenic
TTGTTATGTTCATCAAGGCA+AGG 0.603816 5_4:+6133537 None:intergenic
TGTATATTGTGATTCCTATG+TGG 0.604015 5_4:-6132780 Msa0832960:CDS
ATATCAGCCTAATCATACTT+GGG 0.606198 5_4:-6132120 Msa0832960:CDS
AAGGTAGCCCTTTATAACAA+TGG 0.607429 5_4:-6135532 Msa0832960:CDS
GAGTGAACACATAGGAGGTG+TGG 0.608475 5_4:-6132042 Msa0832960:CDS
TAATGTTTCCACCAACATCA+GGG 0.608720 5_4:+6131838 None:intergenic
CAGAATTGGAATCGATCGAA+CGG 0.618474 5_4:+6133395 None:intergenic
ATGGTTATAGAATGTCCCTA+TGG 0.625370 5_4:-6132064 Msa0832960:CDS
CCAGTTGAAAAGTTATGTGA+TGG 0.628489 5_4:-6135655 Msa0832960:CDS
TTGGAATGCAAATCATGCCA+TGG 0.632340 5_4:+6133717 None:intergenic
TCCATATGTCACGGTTTCTG+TGG 0.632651 5_4:-6135839 Msa0832960:CDS
ACATTCTATAACCATGCACC+TGG 0.636369 5_4:+6132072 None:intergenic
AACCGAAAGAGGCTATACAA+AGG 0.636642 5_4:-6133354 Msa0832960:CDS
AACGCTCCTCAAAATTGGTG+AGG 0.637134 5_4:+6133667 None:intergenic
CATGATGTGAGTTATGAGAG+TGG 0.638733 5_4:-6135379 Msa0832960:CDS
TAACCATGCACCTGGCCATG+AGG 0.638841 5_4:+6132080 None:intergenic
AACCAGTGATCCATATGTCA+CGG 0.640470 5_4:-6135848 Msa0832960:CDS
AAGAAATTCAAGTAGTCCTG+TGG 0.640692 5_4:+6132477 None:intergenic
TAGAGACACAGAAATTGCAA+TGG 0.640880 5_4:-6132147 Msa0832960:CDS
ATAAACCAAAGATCAATGGA+AGG 0.645459 5_4:-6132173 Msa0832960:CDS
TTGCCTTGATGAACATAACA+AGG 0.649129 5_4:-6133535 Msa0832960:CDS
AATTTACAAAGCATTAGAGG+AGG 0.674412 5_4:-6132523 Msa0832960:CDS
CTGGTTGTCCAAGACAACCA+TGG 0.675621 5_4:-6133734 Msa0832960:CDS
CTATGGAGTGAACACATAGG+AGG 0.681727 5_4:-6132047 Msa0832960:CDS
CTTTCATAATCCTAACTACG+AGG 0.683265 5_4:-6133886 Msa0832960:intron
ACTTATGAACACTTATGACG+AGG 0.684284 5_4:-6134994 Msa0832960:CDS
GAGACTAACATTGTAGTACA+CGG 0.710080 5_4:+6135288 None:intergenic
CCTCAAGTACAGCTACTCAG+AGG 0.711303 5_4:-6132744 Msa0832960:CDS
TCCCTATGGAGTGAACACAT+AGG 0.736923 5_4:-6132050 Msa0832960:CDS
AAACTAGGAAGAGAACCTTG+TGG 0.820813 5_4:+6135421 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATAATGAAAATAGAAAA+TGG + chr5_4:6134029-6134048 None:intergenic 15.0%
!!! ATGTTCATATGAAATTTTTA+TGG + chr5_4:6135231-6135250 None:intergenic 15.0%
!! GATAATGAAAATATTTCAAA+TGG - chr5_4:6135452-6135471 Msa0832960:CDS 15.0%
!! TTTCCATTAGTATTAAGAAT+GGG + chr5_4:6132274-6132293 None:intergenic 20.0%
!!! TTTTCCATTAGTATTAAGAA+TGG + chr5_4:6132275-6132294 None:intergenic 20.0%
!! TTAATCTTTCACTATTCTTT+CGG - chr5_4:6133555-6133574 Msa0832960:CDS 20.0%
!! TTTAAGAATATCTGTATTAC+TGG + chr5_4:6133728-6133747 None:intergenic 20.0%
!! TTTAAATACAGTATACTACT+CGG + chr5_4:6133768-6133787 None:intergenic 20.0%
!! GTCTGAAAATATATTATAAG+TGG - chr5_4:6134079-6134098 Msa0832960:intron 20.0%
!! TGAAAATATATTATAAGTGG+TGG - chr5_4:6134082-6134101 Msa0832960:intron 20.0%
!!! ATTGAGAATCAATATTTTCT+TGG - chr5_4:6134741-6134760 Msa0832960:intron 20.0%
!! AATGATTAACTAACTTAATC+AGG + chr5_4:6135570-6135589 None:intergenic 20.0%
!! ATGATATATGTTCATTCAAA+AGG - chr5_4:6135642-6135661 Msa0832960:CDS 20.0%
!! ATTTTGTTGCCAAGTTTTTT+AGG + chr5_4:6132007-6132026 None:intergenic 25.0%
! TTCCATTAGTATTAAGAATG+GGG + chr5_4:6132273-6132292 None:intergenic 25.0%
!! ACATATTTTCCACTTAGAAA+AGG - chr5_4:6132403-6132422 Msa0832960:CDS 25.0%
! AAGCATGTTTGGATATAAAA+CGG - chr5_4:6132726-6132745 Msa0832960:intron 25.0%
!!! ACTATGATTTGTTGATATGT+AGG - chr5_4:6132860-6132879 Msa0832960:intron 25.0%
! AAACATAAGTACACTAGTTA+AGG - chr5_4:6133144-6133163 Msa0832960:CDS 25.0%
!!! TTGATCAATCATATTGATTC+TGG - chr5_4:6133275-6133294 Msa0832960:intron 25.0%
! GAAGTCAATAATACAAACTT+GGG + chr5_4:6133476-6133495 None:intergenic 25.0%
! TGAAGTCAATAATACAAACT+TGG + chr5_4:6133477-6133496 None:intergenic 25.0%
! TAATCTTTCACTATTCTTTC+GGG - chr5_4:6133556-6133575 Msa0832960:CDS 25.0%
!! ATTTGTTAGAAGACTAACTA+TGG + chr5_4:6133677-6133696 None:intergenic 25.0%
! AATTGATCAGAAAGCAAAAT+AGG + chr5_4:6133797-6133816 None:intergenic 25.0%
! AGAAAGATTATAGCTTTCAT+TGG - chr5_4:6133891-6133910 Msa0832960:CDS 25.0%
! ATATTGAAGAAGTTTCTCAA+CGG + chr5_4:6134413-6134432 None:intergenic 25.0%
! TGCAAACAATTTAATTCCAA+TGG - chr5_4:6135027-6135046 Msa0832960:intron 25.0%
! TGTCAATATCAAACATGTAT+AGG + chr5_4:6135308-6135327 None:intergenic 25.0%
!! AACAATTTACAAAGCATTAG+AGG - chr5_4:6135355-6135374 Msa0832960:CDS 25.0%
! TCAAATGGTGTAAAATCTAA+CGG - chr5_4:6135467-6135486 Msa0832960:CDS 25.0%
! GATTAAAAACAAAATACCTG+AGG + chr5_4:6135503-6135522 None:intergenic 25.0%
!! TCATTATTGTTTGCACATTT+TGG - chr5_4:6135585-6135604 Msa0832960:CDS 25.0%
!! ATTTTCTTGAAGTACAGTAA+AGG + chr5_4:6136075-6136094 None:intergenic 25.0%
CTTAGAAATTTGCATTCAAG+AGG - chr5_4:6131919-6131938 Msa0832960:CDS 30.0%
TCAATCCTACCTAAAAAACT+TGG - chr5_4:6131995-6132014 Msa0832960:CDS 30.0%
TAAAATGTTGCATCCAAACA+GGG + chr5_4:6132114-6132133 None:intergenic 30.0%
TTAAAATGTTGCATCCAAAC+AGG + chr5_4:6132115-6132134 None:intergenic 30.0%
GTAAAAGACAGTGATATTGT+TGG - chr5_4:6132175-6132194 Msa0832960:CDS 30.0%
TAGTATTAAGAATGGGGAAA+AGG + chr5_4:6132267-6132286 None:intergenic 30.0%
TTCCCCATTCTTAATACTAA+TGG - chr5_4:6132268-6132287 Msa0832960:CDS 30.0%
CTGAATAGATAAGCTCAAAA+CGG + chr5_4:6132314-6132333 None:intergenic 30.0%
TCAGTATACTCCTATTCATA+AGG - chr5_4:6132330-6132349 Msa0832960:intron 30.0%
ACCAACACCATTGTTATAAA+GGG + chr5_4:6132359-6132378 None:intergenic 30.0%
! TATTTTCCACTTAGAAAAGG+TGG - chr5_4:6132406-6132425 Msa0832960:CDS 30.0%
TATCTTTGATGCAATAAGTC+AGG - chr5_4:6132540-6132559 Msa0832960:CDS 30.0%
! ACTACAATGTTAGTCTCATT+AGG - chr5_4:6132599-6132618 Msa0832960:intron 30.0%
! CTACAATGTTAGTCTCATTA+GGG - chr5_4:6132600-6132619 Msa0832960:intron 30.0%
GATGGAAAAGATTGTACATT+AGG - chr5_4:6132631-6132650 Msa0832960:intron 30.0%
GCAGGTAAGAATAAATAGAA+AGG + chr5_4:6132769-6132788 None:intergenic 30.0%
TTCAAAATGTAACTACTTGC+AGG + chr5_4:6132787-6132806 None:intergenic 30.0%
GCAACTGTTTGCAAATTAAT+AGG + chr5_4:6132821-6132840 None:intergenic 30.0%
!! TGATTTGTTGATATGTAGGT+TGG - chr5_4:6132864-6132883 Msa0832960:intron 30.0%
TGTCTATTGAGTGCATAATA+TGG - chr5_4:6133024-6133043 Msa0832960:intron 30.0%
TGAGTGCATAATATGGTTTA+AGG - chr5_4:6133031-6133050 Msa0832960:intron 30.0%
GCTCGTTGAATTTAAACTAA+CGG + chr5_4:6133195-6133214 None:intergenic 30.0%
AGAGATGAATGCGAAAAAAT+AGG - chr5_4:6133248-6133267 Msa0832960:intron 30.0%
! AGCATTTCGCATTTTGAATA+TGG + chr5_4:6133406-6133425 None:intergenic 30.0%
TCAATAATACAAACTTGGGT+GGG + chr5_4:6133472-6133491 None:intergenic 30.0%
ACAGATATATGCAGAATTCA+CGG + chr5_4:6133520-6133539 None:intergenic 30.0%
! GCATATATCTGTTTTGTCAT+TGG - chr5_4:6133528-6133547 Msa0832960:CDS 30.0%
AAGATTATAGCTTTCATTGG+AGG - chr5_4:6133894-6133913 Msa0832960:CDS 30.0%
ATTGTCTTCACCTTACAAAT+CGG - chr5_4:6134105-6134124 Msa0832960:intron 30.0%
TATTGAAGAAGTTTCTCAAC+GGG + chr5_4:6134412-6134431 None:intergenic 30.0%
TTCTTGGCTCATCATATAAT+TGG - chr5_4:6134757-6134776 Msa0832960:intron 30.0%
TCTTGGCTCATCATATAATT+GGG - chr5_4:6134758-6134777 Msa0832960:intron 30.0%
AATACTACAATACTTGACGA+GGG + chr5_4:6134937-6134956 None:intergenic 30.0%
AAATACTACAATACTTGACG+AGG + chr5_4:6134938-6134957 None:intergenic 30.0%
GCAAAGGAATGAATATAACA+AGG + chr5_4:6134995-6135014 None:intergenic 30.0%
!! ATCTTTAGAGCAATTTCCAT+TGG + chr5_4:6135046-6135065 None:intergenic 30.0%
TGTATATTGTGATTCCTATG+TGG - chr5_4:6135101-6135120 Msa0832960:intron 30.0%
TCCTTTACCACTTGAAAAAA+TGG - chr5_4:6135182-6135201 Msa0832960:CDS 30.0%
!!! ACCATTTTTTCAAGTGGTAA+AGG + chr5_4:6135186-6135205 None:intergenic 30.0%
!!! TTTGTCACCATTTTTTCAAG+TGG + chr5_4:6135192-6135211 None:intergenic 30.0%
TGACAATGTTATGTTGTTCA+AGG + chr5_4:6135260-6135279 None:intergenic 30.0%
AAGACAATGCAAATGATGTA+TGG - chr5_4:6135332-6135351 Msa0832960:CDS 30.0%
! AATTTACAAAGCATTAGAGG+AGG - chr5_4:6135358-6135377 Msa0832960:CDS 30.0%
! TACTTGAATTTCTTTTGCCT+AGG - chr5_4:6135413-6135432 Msa0832960:CDS 30.0%
TCATTCAAAAGGAATGATAG+TGG - chr5_4:6135653-6135672 Msa0832960:CDS 30.0%
GATGATGAATACGTGTTAAT+GGG - chr5_4:6135675-6135694 Msa0832960:CDS 30.0%
! CATTGATCTTTGGTTTATGT+TGG + chr5_4:6135706-6135725 None:intergenic 30.0%
CAACATAAACCAAAGATCAA+TGG - chr5_4:6135704-6135723 Msa0832960:CDS 30.0%
ATAAACCAAAGATCAATGGA+AGG - chr5_4:6135708-6135727 Msa0832960:CDS 30.0%
ATATCAGCCTAATCATACTT+GGG - chr5_4:6135761-6135780 Msa0832960:CDS 30.0%
TTGAATGTGTGAGAAGAATA+AGG - chr5_4:6135889-6135908 Msa0832960:CDS 30.0%
GTATAACTGGAGACAATATT+TGG - chr5_4:6135923-6135942 Msa0832960:CDS 30.0%
! TATGGTGAAACTCCATCTTT+TGG - chr5_4:6131812-6131831 Msa0832960:CDS 35.0%
AACTTGAAGTGGTTGGAAAT+GGG + chr5_4:6131871-6131890 None:intergenic 35.0%
ATGTAAGAGTAGGATCCTTA+AGG + chr5_4:6131894-6131913 None:intergenic 35.0%
TTAAGGATCCTACTCTTACA+TGG - chr5_4:6131893-6131912 Msa0832960:CDS 35.0%
!! TTGTGAAAAGTGTCCATGTT+AGG + chr5_4:6131956-6131975 None:intergenic 35.0%
! GACACTTTTCACAAGAACTT+GGG - chr5_4:6131962-6131981 Msa0832960:CDS 35.0%
! ACACTTTTCACAAGAACTTG+GGG - chr5_4:6131963-6131982 Msa0832960:CDS 35.0%
!!! TGTTGCCAAGTTTTTTAGGT+AGG + chr5_4:6132003-6132022 None:intergenic 35.0%
!! ATTGTTGGTTCACAGCTTAT+TGG - chr5_4:6132190-6132209 Msa0832960:CDS 35.0%
! CCATCACATAACTTTTCAAC+TGG + chr5_4:6132229-6132248 None:intergenic 35.0%
CCAGTTGAAAAGTTATGTGA+TGG - chr5_4:6132226-6132245 Msa0832960:CDS 35.0%
AAAACGGTTTCAGTCTTGAA+GGG + chr5_4:6132298-6132317 None:intergenic 35.0%
TAAAGGGCTACCTTATGAAT+AGG + chr5_4:6132343-6132362 None:intergenic 35.0%
AAGGTAGCCCTTTATAACAA+TGG - chr5_4:6132349-6132368 Msa0832960:intron 35.0%
CACCAACACCATTGTTATAA+AGG + chr5_4:6132360-6132379 None:intergenic 35.0%
! CTTTATAACAATGGTGTTGG+TGG - chr5_4:6132358-6132377 Msa0832960:intron 35.0%
CTAAGTGGAAAATATGTAGC+AGG + chr5_4:6132400-6132419 None:intergenic 35.0%
! ACTTTTCCACCTTTTCTAAG+TGG + chr5_4:6132415-6132434 None:intergenic 35.0%
GTTATGAGAGTGGAAATTGT+TGG - chr5_4:6132512-6132531 Msa0832960:CDS 35.0%
GAGACTAACATTGTAGTACA+CGG + chr5_4:6132596-6132615 None:intergenic 35.0%
!! CCAAACATGCTTTTAGAAGT+AGG + chr5_4:6132718-6132737 None:intergenic 35.0%
! CCTACTTCTAAAAGCATGTT+TGG - chr5_4:6132715-6132734 Msa0832960:intron 35.0%
ACTTATGAACACTTATGACG+AGG - chr5_4:6132887-6132906 Msa0832960:intron 35.0%
TTTCAAGCACTCTTCAGTTA+AGG - chr5_4:6132923-6132942 Msa0832960:intron 35.0%
CTAACTATACATGAGAGAGT+GGG - chr5_4:6133298-6133317 Msa0832960:intron 35.0%
TGATATATATGATGCCACAC+AGG - chr5_4:6133332-6133351 Msa0832960:intron 35.0%
AGAAATGCAAAAATCCTGTG+TGG + chr5_4:6133349-6133368 None:intergenic 35.0%
GTCAATAATACAAACTTGGG+TGG + chr5_4:6133473-6133492 None:intergenic 35.0%
ACGGCATGTTGTTGAATAAA+AGG + chr5_4:6133501-6133520 None:intergenic 35.0%
GTTAGAAGACTAACTATGGA+TGG + chr5_4:6133673-6133692 None:intergenic 35.0%
TTGCTTTCTGATCAATTGCT+AGG - chr5_4:6133800-6133819 Msa0832960:intron 35.0%
CTTTCATAATCCTAACTACG+AGG - chr5_4:6133995-6134014 Msa0832960:CDS 35.0%
GTCTCTCATCGGATAAAATA+TGG - chr5_4:6134057-6134076 Msa0832960:CDS 35.0%
TTGTCTTCACCTTACAAATC+GGG - chr5_4:6134106-6134125 Msa0832960:intron 35.0%
!! CTTACAAATCGGGTTTTGTA+GGG - chr5_4:6134116-6134135 Msa0832960:intron 35.0%
GATTTGCATTCCAAAGTTGA+TGG - chr5_4:6134173-6134192 Msa0832960:intron 35.0%
AAAATTGGTGAGGATGTCAT+AGG + chr5_4:6134207-6134226 None:intergenic 35.0%
GACAGAATCTCTGACATTAT+TGG - chr5_4:6134308-6134327 Msa0832960:intron 35.0%
TTGTTATGTTCATCAAGGCA+AGG + chr5_4:6134347-6134366 None:intergenic 35.0%
TTGCCTTGATGAACATAACA+AGG - chr5_4:6134346-6134365 Msa0832960:intron 35.0%
! TCTCCTTGTTATGTTCATCA+AGG + chr5_4:6134352-6134371 None:intergenic 35.0%
ATGAACATAACAAGGAGACT+TGG - chr5_4:6134354-6134373 Msa0832960:intron 35.0%
! ATCGATTCCAATTCTGTGAA+AGG - chr5_4:6134493-6134512 Msa0832960:intron 35.0%
TGGAAATCCTTTCACAGAAT+TGG + chr5_4:6134503-6134522 None:intergenic 35.0%
ATTTCCAAAAGAACCGAAAG+AGG - chr5_4:6134516-6134535 Msa0832960:intron 35.0%
CTTCATTCTCTTCTCAAACT+TGG - chr5_4:6134559-6134578 Msa0832960:intron 35.0%
CTCTTTGATTAACACTTCAC+AGG + chr5_4:6134598-6134617 None:intergenic 35.0%
TTGACTTCGTTATCAGAACT+TGG - chr5_4:6134635-6134654 Msa0832960:intron 35.0%
!! CATTCCTTTGCTTTTATGAC+AGG - chr5_4:6135004-6135023 Msa0832960:intron 35.0%
TGAAAATGAGTATGAACCAC+AGG - chr5_4:6135388-6135407 Msa0832960:CDS 35.0%
AAGAAATTCAAGTAGTCCTG+TGG + chr5_4:6135407-6135426 None:intergenic 35.0%
TAACGGAAAAAACAATCCTC+AGG - chr5_4:6135484-6135503 Msa0832960:CDS 35.0%
AACTAACTTAATCAGGAAGC+AGG + chr5_4:6135563-6135582 None:intergenic 35.0%
!! GTTTGCACATTTTGGAAACT+AGG - chr5_4:6135593-6135612 Msa0832960:CDS 35.0%
GGATGATGAATACGTGTTAA+TGG - chr5_4:6135674-6135693 Msa0832960:CDS 35.0%
TAGAGACACAGAAATTGCAA+TGG - chr5_4:6135734-6135753 Msa0832960:CDS 35.0%
AGAGACACAGAAATTGCAAT+GGG - chr5_4:6135735-6135754 Msa0832960:CDS 35.0%
CATATCAGCCTAATCATACT+TGG - chr5_4:6135760-6135779 Msa0832960:CDS 35.0%
ACCAAGAAATCTAAACCTCA+TGG - chr5_4:6135786-6135805 Msa0832960:CDS 35.0%
ATGGTTATAGAATGTCCCTA+TGG - chr5_4:6135817-6135836 Msa0832960:CDS 35.0%
!! TCCACTTGATGTTGATTCAA+AGG - chr5_4:6135986-6136005 Msa0832960:CDS 35.0%
!! CCACTTGATGTTGATTCAAA+GGG - chr5_4:6135987-6136006 Msa0832960:CDS 35.0%
TAATGTTTCCACCAACATCA+GGG + chr5_4:6136046-6136065 None:intergenic 35.0%
TTAATGTTTCCACCAACATC+AGG + chr5_4:6136047-6136066 None:intergenic 35.0%
! TGATGTTGGTGGAAACATTA+AGG - chr5_4:6136046-6136065 Msa0832960:CDS 35.0%
! GATGTTGGTGGAAACATTAA+GGG - chr5_4:6136047-6136066 Msa0832960:CDS 35.0%
! TGGGTTGAGTTTCCAAAAGA+TGG + chr5_4:6131827-6131846 None:intergenic 40.0%
!! TCTTTTGGAAACTCAACCCA+TGG - chr5_4:6131827-6131846 Msa0832960:CDS 40.0%
AAGTTGTTGACCTTGTCCAT+GGG + chr5_4:6131846-6131865 None:intergenic 40.0%
GAACTTGAAGTGGTTGGAAA+TGG + chr5_4:6131872-6131891 None:intergenic 40.0%
CTTAAGGAACTTGAAGTGGT+TGG + chr5_4:6131878-6131897 None:intergenic 40.0%
! CAACCACTTCAAGTTCCTTA+AGG - chr5_4:6131876-6131895 Msa0832960:CDS 40.0%
GATCCTTAAGGAACTTGAAG+TGG + chr5_4:6131882-6131901 None:intergenic 40.0%
CTAAGTTGCCATGTAAGAGT+AGG + chr5_4:6131904-6131923 None:intergenic 40.0%
! GTCCATGTTAGGAAGGTTTT+TGG + chr5_4:6131945-6131964 None:intergenic 40.0%
!! GAAAAGTGTCCATGTTAGGA+AGG + chr5_4:6131952-6131971 None:intergenic 40.0%
! GGACACTTTTCACAAGAACT+TGG - chr5_4:6131961-6131980 Msa0832960:CDS 40.0%
! CACTTTTCACAAGAACTTGG+GGG - chr5_4:6131964-6131983 Msa0832960:CDS 40.0%
! TGACATATGGATCACTGGTT+TGG + chr5_4:6132033-6132052 None:intergenic 40.0%
AACCAGTGATCCATATGTCA+CGG - chr5_4:6132033-6132052 Msa0832960:CDS 40.0%
GAAATGATGAGAACCCTGTT+TGG - chr5_4:6132098-6132117 Msa0832960:CDS 40.0%
! TGTGATGGTGCAAAAGTTGA+AGG - chr5_4:6132241-6132260 Msa0832960:CDS 40.0%
CAAAACGGTTTCAGTCTTGA+AGG + chr5_4:6132299-6132318 None:intergenic 40.0%
GCCCTTTATAACAATGGTGT+TGG - chr5_4:6132355-6132374 Msa0832960:intron 40.0%
AAACTAGGAAGAGAACCTTG+TGG + chr5_4:6132463-6132482 None:intergenic 40.0%
AAGGTTCTCTTCCTAGTTTG+CGG - chr5_4:6132464-6132483 Msa0832960:CDS 40.0%
CATGATGTGAGTTATGAGAG+TGG - chr5_4:6132502-6132521 Msa0832960:CDS 40.0%
!! GATTGGTCTATATCGTTGGT+TGG - chr5_4:6132569-6132588 Msa0832960:intron 40.0%
CTCATTAGGGATACTAGAGA+TGG - chr5_4:6132613-6132632 Msa0832960:intron 40.0%
CTCAAAGCCAAATCACAAGA+AGG - chr5_4:6132661-6132680 Msa0832960:intron 40.0%
TCTCACACCTTCTTGTGATT+TGG + chr5_4:6132671-6132690 None:intergenic 40.0%
GCTAACTATACATGAGAGAG+TGG - chr5_4:6133297-6133316 Msa0832960:intron 40.0%
ATACAAACTTGGGTGGGATA+CGG + chr5_4:6133466-6133485 None:intergenic 40.0%
ATTGCTATATCCAGCGAAAG+AGG + chr5_4:6133586-6133605 None:intergenic 40.0%
TCATCAGAAGACAGTCATAG+TGG - chr5_4:6133845-6133864 Msa0832960:intron 40.0%
GGAGGACTTGATTTATGTCT+CGG - chr5_4:6133912-6133931 Msa0832960:CDS 40.0%
CTCCAACACATTCTCTCTTT+AGG - chr5_4:6133946-6133965 Msa0832960:CDS 40.0%
GTCCTAAAGAGAGAATGTGT+TGG + chr5_4:6133951-6133970 None:intergenic 40.0%
TTCTCTTGAGAGTCTCTCAT+CGG - chr5_4:6134046-6134065 Msa0832960:CDS 40.0%
! CCTACAAAACCCGATTTGTA+AGG + chr5_4:6134118-6134137 None:intergenic 40.0%
!! CCTTACAAATCGGGTTTTGT+AGG - chr5_4:6134115-6134134 Msa0832960:intron 40.0%
! GTTTTGTAGGGTTCTGTTAC+TGG - chr5_4:6134128-6134147 Msa0832960:intron 40.0%
AAATCATGCCATGGTTGTCT+TGG + chr5_4:6134158-6134177 None:intergenic 40.0%
TTGGAATGCAAATCATGCCA+TGG + chr5_4:6134167-6134186 None:intergenic 40.0%
! TGACATCCTCACCAATTTTG+AGG - chr5_4:6134208-6134227 Msa0832960:intron 40.0%
CAGAATTGGAATCGATCGAA+CGG + chr5_4:6134489-6134508 None:intergenic 40.0%
! ATAGCCTCTTTCGGTTCTTT+TGG + chr5_4:6134523-6134542 None:intergenic 40.0%
AACCGAAAGAGGCTATACAA+AGG - chr5_4:6134527-6134546 Msa0832960:intron 40.0%
ACCGAAAGAGGCTATACAAA+GGG - chr5_4:6134528-6134547 Msa0832960:intron 40.0%
ACCCTTTGTATAGCCTCTTT+CGG + chr5_4:6134532-6134551 None:intergenic 40.0%
! CGTTATCAGAACTTGGTTTG+TGG - chr5_4:6134642-6134661 Msa0832960:intron 40.0%
TGGGATTCTTACAAAGACCT+TGG - chr5_4:6134777-6134796 Msa0832960:intron 40.0%
TTTGAGCTCAATACTCACCA+AGG + chr5_4:6134797-6134816 None:intergenic 40.0%
TGAGCTCAAAAACGAAGAGA+CGG - chr5_4:6134807-6134826 Msa0832960:intron 40.0%
TGCACCTGTCATAAAAGCAA+AGG + chr5_4:6135011-6135030 None:intergenic 40.0%
! CTACTCAGAGGATTCTCTTT+TGG - chr5_4:6135149-6135168 Msa0832960:intron 40.0%
TAGTGCCTTCCATTGATCTT+TGG + chr5_4:6135716-6135735 None:intergenic 40.0%
TTGGTTGCCCAAGTATGATT+AGG + chr5_4:6135771-6135790 None:intergenic 40.0%
GCCATGAGGTTTAGATTTCT+TGG + chr5_4:6135790-6135809 None:intergenic 40.0%
ACATTCTATAACCATGCACC+TGG + chr5_4:6135812-6135831 None:intergenic 40.0%
TCCTATGTGTTCACTCCATA+GGG + chr5_4:6135835-6135854 None:intergenic 40.0%
GGTCATTGAGTGAGTATAAC+TGG - chr5_4:6135910-6135929 Msa0832960:CDS 40.0%
CCCTTTGAATCAACATCAAG+TGG + chr5_4:6135990-6136009 None:intergenic 40.0%
CAAGTTGTTGACCTTGTCCA+TGG + chr5_4:6131847-6131866 None:intergenic 45.0%
GGCCAAAAACCTTCCTAACA+TGG - chr5_4:6131940-6131959 Msa0832960:CDS 45.0%
AACCGTGACATATGGATCAC+TGG + chr5_4:6132038-6132057 None:intergenic 45.0%
TCCATATGTCACGGTTTCTG+TGG - chr5_4:6132042-6132061 Msa0832960:CDS 45.0%
TCTGATGCTTGATGTGCAAC+AGG + chr5_4:6132142-6132161 None:intergenic 45.0%
TCACAGCTTATTGGTGCAGT+TGG - chr5_4:6132199-6132218 Msa0832960:CDS 45.0%
!!! GGTGTTGGTGGTGATTTTCA+AGG - chr5_4:6132370-6132389 Msa0832960:intron 45.0%
CAAGATGCTCATGTTCCACA+AGG - chr5_4:6132445-6132464 Msa0832960:CDS 45.0%
! AGGTTCTCTTCCTAGTTTGC+GGG - chr5_4:6132465-6132484 Msa0832960:CDS 45.0%
!! CGTCGATTGGTCTATATCGT+TGG - chr5_4:6132565-6132584 Msa0832960:intron 45.0%
!! TGAGAGTGCTTCTACTTGTG+TGG - chr5_4:6132686-6132705 Msa0832960:intron 45.0%
!! GAGAGTGCTTCTACTTGTGT+GGG - chr5_4:6132687-6132706 Msa0832960:intron 45.0%
! GTCCTTTTCCTCCAGATCTT+GGG + chr5_4:6132959-6132978 None:intergenic 45.0%
! TGTCCTTTTCCTCCAGATCT+TGG + chr5_4:6132960-6132979 None:intergenic 45.0%
! GCGAAATGCTGTTTATAGCG+AGG - chr5_4:6133416-6133435 Msa0832960:intron 45.0%
CTGATCAATTGCTAGGAAGC+TGG - chr5_4:6133807-6133826 Msa0832960:intron 45.0%
GGATGACTAACCTCGTAGTT+AGG + chr5_4:6134008-6134027 None:intergenic 45.0%
AACGCTCCTCAAAATTGGTG+AGG + chr5_4:6134217-6134236 None:intergenic 45.0%
!! TCACCAATTTTGAGGAGCGT+TGG - chr5_4:6134216-6134235 Msa0832960:intron 45.0%
CAACCAACGCTCCTCAAAAT+TGG + chr5_4:6134222-6134241 None:intergenic 45.0%
!!! TTTTGAGGAGCGTTGGTTGA+TGG - chr5_4:6134223-6134242 Msa0832960:intron 45.0%
CTCAAAAACGAAGAGACGGA+GGG - chr5_4:6134811-6134830 Msa0832960:intron 45.0%
TCAAAAACGAAGAGACGGAG+GGG - chr5_4:6134812-6134831 Msa0832960:intron 45.0%
CTCTGAGTAGCTGTACTTGA+GGG + chr5_4:6135139-6135158 None:intergenic 45.0%
! TCAGAGGATTCTCTTTTGGC+AGG - chr5_4:6135153-6135172 Msa0832960:intron 45.0%
! TACAAAGCATTAGAGGAGGC+TGG - chr5_4:6135362-6135381 Msa0832960:CDS 45.0%
AATCAGGAAGCAGGCACAAA+AGG + chr5_4:6135554-6135573 None:intergenic 45.0%
GAAATCTAAACCTCATGGCC+AGG - chr5_4:6135791-6135810 Msa0832960:CDS 45.0%
TCCCTATGGAGTGAACACAT+AGG - chr5_4:6135831-6135850 Msa0832960:CDS 45.0%
CTCCTATGTGTTCACTCCAT+AGG + chr5_4:6135836-6135855 None:intergenic 45.0%
CTATGGAGTGAACACATAGG+AGG - chr5_4:6135834-6135853 Msa0832960:CDS 45.0%
CTCACACATTCAATGCTCTC+TGG + chr5_4:6135882-6135901 None:intergenic 45.0%
AAGGTTTCACAGCCAACAAG+AGG + chr5_4:6136023-6136042 None:intergenic 45.0%
TGTGAAACCTTCCCTGATGT+TGG - chr5_4:6136032-6136051 Msa0832960:CDS 45.0%
!! TTAAAAATATTACATTATAT+AGG - chr5_4:6134956-6134975 Msa0832960:CDS 5.0%
GGAAACTCAACCCATGGACA+AGG - chr5_4:6131833-6131852 Msa0832960:CDS 50.0%
GCCACAGAAACCGTGACATA+TGG + chr5_4:6132046-6132065 None:intergenic 50.0%
TATGTCACGGTTTCTGTGGC+TGG - chr5_4:6132046-6132065 Msa0832960:CDS 50.0%
! CTTCCTAGTTTGCGGGTTGA+TGG - chr5_4:6132472-6132491 Msa0832960:CDS 50.0%
! AATAAGTCAGGCGCGTCGAT+TGG - chr5_4:6132552-6132571 Msa0832960:CDS 50.0%
GTGCTTCTTTGCCCAAGATC+TGG - chr5_4:6132945-6132964 Msa0832960:intron 50.0%
CTTCTTTGCCCAAGATCTGG+AGG - chr5_4:6132948-6132967 Msa0832960:intron 50.0%
TGCCCAAGATCTGGAGGAAA+AGG - chr5_4:6132954-6132973 Msa0832960:intron 50.0%
CTGGAGGAAAAGGACATAGC+TGG - chr5_4:6132964-6132983 Msa0832960:intron 50.0%
! ACATAGCTGGCTCAAACAGC+AGG - chr5_4:6132977-6132996 Msa0832960:intron 50.0%
ACTAACGGCAGTCGAGCAAT+AGG + chr5_4:6133180-6133199 None:intergenic 50.0%
AAACTTGGGTGGGATACGGT+CGG + chr5_4:6133462-6133481 None:intergenic 50.0%
!! GTCATAGTGGATGCTGATGC+TGG - chr5_4:6133858-6133877 Msa0832960:intron 50.0%
CTGGTTGTCCAAGACAACCA+TGG - chr5_4:6134147-6134166 Msa0832960:intron 50.0%
CAAGGAGACTTGGCATGTTC+AGG - chr5_4:6134364-6134383 Msa0832960:intron 50.0%
GCTCAAAAACGAAGAGACGG+AGG - chr5_4:6134810-6134829 Msa0832960:intron 50.0%
GTGATTCCTATGTGGCCTGA+AGG - chr5_4:6135109-6135128 Msa0832960:intron 50.0%
! GTACTTGAGGGTACACCTTC+AGG + chr5_4:6135127-6135146 None:intergenic 50.0%
CCTCTGAGTAGCTGTACTTG+AGG + chr5_4:6135140-6135159 None:intergenic 50.0%
CCTCAAGTACAGCTACTCAG+AGG - chr5_4:6135137-6135156 Msa0832960:intron 50.0%
GAGTGAACACATAGGAGGTG+TGG - chr5_4:6135839-6135858 Msa0832960:CDS 50.0%
GGCATTGTGAAGCCTCTTGT+TGG - chr5_4:6136008-6136027 Msa0832960:CDS 50.0%
GAAACCTTCCCTGATGTTGG+TGG - chr5_4:6136035-6136054 Msa0832960:CDS 50.0%
GTTTCCACCAACATCAGGGA+AGG + chr5_4:6136042-6136061 None:intergenic 50.0%
CCACCATCAACCCGCAAACT+AGG + chr5_4:6132478-6132497 None:intergenic 55.0%
!! CCTAGTTTGCGGGTTGATGG+TGG - chr5_4:6132475-6132494 Msa0832960:CDS 55.0%
AACTTGGGTGGGATACGGTC+GGG + chr5_4:6133461-6133480 None:intergenic 55.0%
TTCGGGACAACCTCTTTCGC+TGG - chr5_4:6133573-6133592 Msa0832960:CDS 55.0%
GGCAGCAGGACCATCAACTT+TGG + chr5_4:6134186-6134205 None:intergenic 55.0%
GTGAGGATGTCATAGGCAGC+AGG + chr5_4:6134200-6134219 None:intergenic 55.0%
GGTACACCTTCAGGCCACAT+AGG + chr5_4:6135118-6135137 None:intergenic 55.0%
AAACCTCATGGCCAGGTGCA+TGG - chr5_4:6135798-6135817 Msa0832960:CDS 55.0%
TAACCATGCACCTGGCCATG+AGG + chr5_4:6135804-6135823 None:intergenic 55.0%
CCTAGGCAATCGCGAGCTCT+CGG - chr5_4:6135430-6135449 Msa0832960:CDS 60.0%
CCGAGAGCTCGCGATTGCCT+AGG + chr5_4:6135433-6135452 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 6131797 6136106 6131797 ID=Msa0832960;Name=Msa0832960
chr5_4 mRNA 6131797 6136106 6131797 ID=Msa0832960-mRNA-1;Parent=Msa0832960;Name=Msa0832960-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0.12|0|1|1|1|9|0|848
chr5_4 exon 6135156 6136106 6135156 ID=Msa0832960-mRNA-1:exon:2658;Parent=Msa0832960-mRNA-1
chr5_4 exon 6134905 6135021 6134905 ID=Msa0832960-mRNA-1:exon:2657;Parent=Msa0832960-mRNA-1
chr5_4 exon 6133887 6134081 6133887 ID=Msa0832960-mRNA-1:exon:2656;Parent=Msa0832960-mRNA-1
chr5_4 exon 6133518 6133766 6133518 ID=Msa0832960-mRNA-1:exon:2655;Parent=Msa0832960-mRNA-1
chr5_4 exon 6133354 6133422 6133354 ID=Msa0832960-mRNA-1:exon:2654;Parent=Msa0832960-mRNA-1
chr5_4 exon 6133105 6133252 6133105 ID=Msa0832960-mRNA-1:exon:2653;Parent=Msa0832960-mRNA-1
chr5_4 exon 6132729 6132877 6132729 ID=Msa0832960-mRNA-1:exon:2652;Parent=Msa0832960-mRNA-1
chr5_4 exon 6132398 6132574 6132398 ID=Msa0832960-mRNA-1:exon:2651;Parent=Msa0832960-mRNA-1
chr5_4 exon 6131797 6132288 6131797 ID=Msa0832960-mRNA-1:exon:2650;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6135156 6136106 6135156 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6134905 6135021 6134905 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6133887 6134081 6133887 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6133518 6133766 6133518 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6133354 6133422 6133354 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6133105 6133252 6133105 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6132729 6132877 6132729 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6132398 6132574 6132398 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
chr5_4 CDS 6131797 6132288 6131797 ID=Msa0832960-mRNA-1:cds;Parent=Msa0832960-mRNA-1
Gene Sequence

>Msa0832960

ATGGCTCATTTGGTTTATGGTGAAACTCCATCTTTTGGAAACTCAACCCATGGACAAGGTCAACAACTTGTCCCATTTCCAACCACTTCAAGTTCCTTAAGGATCCTACTCTTACATGGCAACTTAGAAATTTGCATTCAAGAGGCCAAAAACCTTCCTAACATGGACACTTTTCACAAGAACTTGGGGGCAATGCTTTCAATCCTACCTAAAAAACTTGGCAACAAAATGAACCAAACCAGTGATCCATATGTCACGGTTTCTGTGGCTGGTGCTGTGATTGCAAGAACTTTTGTGATCAGAAATGATGAGAACCCTGTTTGGATGCAACATTTTAATGTACCTGTTGCACATCAAGCATCAGAGATTCACTTTGTTGTAAAAGACAGTGATATTGTTGGTTCACAGCTTATTGGTGCAGTTGGAATTCCAGTTGAAAAGTTATGTGATGGTGCAAAAGTTGAAGGCCTTTTCCCCATTCTTAATACTAATGGAAAACCCTTCAAGACTGAAACCGTTTTGAGCTTATCTATTCAGTATACTCCTATTCATAAGGTAGCCCTTTATAACAATGGTGTTGGTGGTGATTTTCAAGGTGTTCCTGCTACATATTTTCCACTTAGAAAAGGTGGAAAAGTTACACTTTATCAAGATGCTCATGTTCCACAAGGTTCTCTTCCTAGTTTGCGGGTTGATGGTGGTGATCATGATGTGAGTTATGAGAGTGGAAATTGTTGGCATGATATCTTTGATGCAATAAGTCAGGCGCGTCGATTGGTCTATATCGTTGGTTGGTCCGTGTACTACAATGTTAGTCTCATTAGGGATACTAGAGATGGAAAAGATTGTACATTAGGAGATCTTCTCAAAGCCAAATCACAAGAAGGTGTGAGAGTGCTTCTACTTGTGTGGGATGATCCTACTTCTAAAAGCATGTTTGGATATAAAACGGTTGGACTTATGAACACTTATGACGAGGAAACTCGCAGTTTTTTCAAGCACTCTTCAGTTAAGGTGCTTCTTTGCCCAAGATCTGGAGGAAAAGGACATAGCTGGCTCAAACAGCAGGAAGCTGGAACAATCTATACTCATCATCAGAAGACAGTCATAGTGGATGCTGATGCTGGTCAGCATAAAAGAAAGATTATAGCTTTCATTGGAGGACTTGATTTATGTCTCGGAAGATATGATACTCCAACACATTCTCTCTTTAGGACTTTGCAGACAACGCACAAAGATGACTTTCATAATCCTAACTACGAGGGTTCTGTTACTGGTTGTCCAAGACAACCATGGCATGATTTGCATTCCAAAGTTGATGGTCCTGCTGCCTATGACATCCTCACCAATTTTGAGGAGCGTTGGTTGATGGCATTAAAAATGACTACACTTCAAAAGATGAAAACTTCACATGATGATTCGTTACTGAAAATTGACAGAATCTCTGACATTATTGGCATTGATGACGTACCTTGCCTTGATGAACATAACAAGGAGACTTGGCATGTTCAGATTTTCCGTTCGATCGATTCCAATTCTGTGAAAGGATTTCCAAAAGAACCGAAAGAGGCTATACAAAGGAACTTGGTTTGTGGAAAGAATGTAATGATAGACATGAGCATACATTCTGCATATGTCAAAGCAATCAGAGCAGCACAGAAGTTCATTTATATTGAGAATCAATATTTTCTTGGCTCATCATATAATTGGGATTCTTACAAAGACCTTGGTGCAAACAATTTAATTCCAATGGAAATTGCTCTAAAGATAGCAAACAAAATCAAACAGAAAGAGAGATTCTGTGTGTATATTGTGATTCCTATGTGGCCTGAAGGTGTACCCTCAAGTACAGCTACTCAGAGGATTCTCTTTTGGCAGTTCAAGACAATGCAAATGATGTATGGAACAATTTACAAAGCATTAGAGGAGGCTGGACTTGAAAATGAGTATGAACCACAGGACTACTTGAATTTCTTTTGCCTAGGCAATCGCGAGCTCTCGGATAATGAAAATATTTCAAATGGTGTAAAATCTAACGGAAAAAACAATCCTCAGGCACTTACTAAAAAGAACAGAAGATTCATGATATATGTTCATTCAAAAGGAATGATAGTGGATGATGAATACGTGTTAATGGGATCTGCCAACATAAACCAAAGATCAATGGAAGGCACTAGAGACACAGAAATTGCAATGGGAGCATATCAGCCTAATCATACTTGGGCAACCAAGAAATCTAAACCTCATGGCCAGGTGCATGGTTATAGAATGTCCCTATGGAGTGAACACATAGGAGGTGTGGAAGAATGTTTCAAGCAACCAGAGAGCATTGAATGTGTGAGAAGAATAAGGTCATTGAGTGAGTATAACTGGAGACAATATTTGGCTGATGAAGTAACTGAAATGAATGCTCATTTACTGAAATATCCACTTGATGTTGATTCAAAGGGCATTGTGAAGCCTCTTGTTGGCTGTGAAACCTTCCCTGATGTTGGTGGAAACATTAAGGGCACCTTTACTGTACTTCAAGAAAATCTCACCATCTAA
Protein sequence

>Msa0832960

MAHLVYGETPSFGNSTHGQGQQLVPFPTTSSSLRILLLHGNLEICIQEAKNLPNMDTFHKNLGAMLSILPKKLGNKMNQTSDPYVTVSVAGAVIARTFVIRNDENPVWMQHFNVPVAHQASEIHFVVKDSDIVGSQLIGAVGIPVEKLCDGAKVEGLFPILNTNGKPFKTETVLSLSIQYTPIHKVALYNNGVGGDFQGVPATYFPLRKGGKVTLYQDAHVPQGSLPSLRVDGGDHDVSYESGNCWHDIFDAISQARRLVYIVGWSVYYNVSLIRDTRDGKDCTLGDLLKAKSQEGVRVLLLVWDDPTSKSMFGYKTVGLMNTYDEETRSFFKHSSVKVLLCPRSGGKGHSWLKQQEAGTIYTHHQKTVIVDADAGQHKRKIIAFIGGLDLCLGRYDTPTHSLFRTLQTTHKDDFHNPNYEGSVTGCPRQPWHDLHSKVDGPAAYDILTNFEERWLMALKMTTLQKMKTSHDDSLLKIDRISDIIGIDDVPCLDEHNKETWHVQIFRSIDSNSVKGFPKEPKEAIQRNLVCGKNVMIDMSIHSAYVKAIRAAQKFIYIENQYFLGSSYNWDSYKDLGANNLIPMEIALKIANKIKQKERFCVYIVIPMWPEGVPSSTATQRILFWQFKTMQMMYGTIYKALEEAGLENEYEPQDYLNFFCLGNRELSDNENISNGVKSNGKNNPQALTKKNRRFMIYVHSKGMIVDDEYVLMGSANINQRSMEGTRDTEIAMGAYQPNHTWATKKSKPHGQVHGYRMSLWSEHIGGVEECFKQPESIECVRRIRSLSEYNWRQYLADEVTEMNAHLLKYPLDVDSKGIVKPLVGCETFPDVGGNIKGTFTVLQENLTI*