AlfalfaGEDB | Msa1450440

M. sativa cultivar zhongmu-4 / Msa1450440

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450440	sp\|Q9LPK5\|CML44_ARATH	41.935	155	84	3	2	155	4	153	1.78e-33	118

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450440	A0A396IXS7	97.484	159	4	0	1	159	6	164	1.66e-103	302

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0030160	Msa1450440	0.803273	3.771577e-49	-8.615850e-47
Msa0032080	Msa1450440	0.802622	5.141566e-49	-8.615850e-47
Msa0041840	Msa1450440	0.802953	4.392878e-49	-8.615850e-47
Msa0051960	Msa1450440	0.804418	2.181453e-49	-8.615850e-47
Msa0052360	Msa1450440	0.824847	6.465374e-54	-8.615850e-47
Msa0060730	Msa1450440	-0.816985	4.168656e-52	-8.615850e-47
Msa0092410	Msa1450440	0.800889	1.165987e-48	-8.615850e-47
Msa0095940	Msa1450440	0.807395	5.162392e-50	-8.615850e-47
Msa0097370	Msa1450440	0.811595	6.471184e-51	-8.615850e-47
Msa0101860	Msa1450440	0.820368	7.116310e-53	-8.615850e-47
Msa0101960	Msa1450440	0.810344	1.208058e-50	-8.615850e-47
Msa0117520	Msa1450440	0.806488	8.027496e-50	-8.615850e-47
Msa1353890	Msa1450440	0.808771	2.628634e-50	-8.615850e-47
Msa1356000	Msa1450440	0.842163	3.058096e-58	-8.615850e-47
Msa1363820	Msa1450440	-0.812638	3.834266e-51	-8.615850e-47
Msa1390290	Msa1450440	0.815797	7.691122e-52	-8.615850e-47
Msa1407760	Msa1450440	0.805520	1.282938e-49	-8.615850e-47
Msa1410550	Msa1450440	0.802563	5.286125e-49	-8.615850e-47
Msa1415410	Msa1450440	0.806760	7.033436e-50	-8.615850e-47
Msa1416730	Msa1450440	0.800175	1.630177e-48	-8.615850e-47
Msa1418970	Msa1450440	0.814129	1.802531e-51	-8.615850e-47
Msa1419690	Msa1450440	0.818384	2.015814e-52	-8.615850e-47
Msa1419840	Msa1450440	0.813660	2.287554e-51	-8.615850e-47
Msa1437730	Msa1450440	0.818793	1.628091e-52	-8.615850e-47
Msa0612080	Msa1450440	0.811977	5.344987e-51	-8.615850e-47
Msa0613420	Msa1450440	0.838224	3.265520e-57	-8.615850e-47
Msa0641130	Msa1450440	0.829236	5.762085e-55	-8.615850e-47
Msa0661720	Msa1450440	0.822709	2.048978e-53	-8.615850e-47
Msa0673120	Msa1450440	0.801445	8.975458e-49	-8.615850e-47
Msa0708310	Msa1450440	0.809593	1.752213e-50	-8.615850e-47
Msa0733010	Msa1450440	0.819755	9.829885e-53	-8.615850e-47
Msa0738030	Msa1450440	0.821120	4.781268e-53	-8.615850e-47
Msa0742210	Msa1450440	0.811813	5.802011e-51	-8.615850e-47
Msa0795340	Msa1450440	0.807235	5.582278e-50	-8.615850e-47
Msa0823370	Msa1450440	0.833219	6.048902e-56	-8.615850e-47
Msa0830230	Msa1450440	0.813493	2.489501e-51	-8.615850e-47
Msa0832950	Msa1450440	0.807448	5.029752e-50	-8.615850e-47
Msa0832960	Msa1450440	0.811017	8.640823e-51	-8.615850e-47
Msa0833270	Msa1450440	0.817996	2.467928e-52	-8.615850e-47
Msa0138020	Msa1450440	0.806207	9.200462e-50	-8.615850e-47
Msa0160960	Msa1450440	0.814914	1.208528e-51	-8.615850e-47
Msa0168310	Msa1450440	0.814347	1.613442e-51	-8.615850e-47
Msa0186320	Msa1450440	0.803919	2.770685e-49	-8.615850e-47
Msa0217700	Msa1450440	0.823070	1.688410e-53	-8.615850e-47
Msa0231060	Msa1450440	0.802727	4.890156e-49	-8.615850e-47
Msa1232700	Msa1450440	0.816341	5.813225e-52	-8.615850e-47
Msa1234470	Msa1450440	0.813827	2.101307e-51	-8.615850e-47
Msa1236440	Msa1450440	0.817088	3.952898e-52	-8.615850e-47
Msa1238500	Msa1450440	0.811946	5.426867e-51	-8.615850e-47
Msa1238600	Msa1450440	0.802925	4.451590e-49	-8.615850e-47
Msa1256060	Msa1450440	0.800871	1.176132e-48	-8.615850e-47
Msa1276520	Msa1450440	0.848367	6.411420e-60	-8.615850e-47
Msa1277670	Msa1450440	0.822882	1.867644e-53	-8.615850e-47
Msa1281690	Msa1450440	0.809342	1.983827e-50	-8.615850e-47
Msa1306960	Msa1450440	0.802731	4.881255e-49	-8.615850e-47
Msa1313280	Msa1450440	0.801792	7.617018e-49	-8.615850e-47
Msa1314750	Msa1450440	0.840562	8.071205e-58	-8.615850e-47
Msa1342380	Msa1450440	0.801276	9.719859e-49	-8.615850e-47
Msa0374890	Msa1450440	0.832185	1.091760e-55	-8.615850e-47
Msa0375070	Msa1450440	0.814673	1.366743e-51	-8.615850e-47
Msa0386010	Msa1450440	0.818688	1.720352e-52	-8.615850e-47
Msa0395750	Msa1450440	0.807290	5.432335e-50	-8.615850e-47
Msa0423860	Msa1450440	0.824230	9.032592e-54	-8.615850e-47
Msa0443010	Msa1450440	0.820254	7.557044e-53	-8.615850e-47
Msa0470360	Msa1450440	0.846390	2.237764e-59	-8.615850e-47
Msa0470880	Msa1450440	0.855110	7.861213e-62	-8.615850e-47
Msa0999680	Msa1450440	0.832260	1.046313e-55	-8.615850e-47
Msa1024640	Msa1450440	0.800120	1.672851e-48	-8.615850e-47
Msa1048220	Msa1450440	0.817444	3.286502e-52	-8.615850e-47
Msa1063360	Msa1450440	0.819895	9.134589e-53	-8.615850e-47
Msa1069190	Msa1450440	0.802971	4.354444e-49	-8.615850e-47
Msa0267790	Msa1450440	0.825924	3.594808e-54	-8.615850e-47
Msa0269560	Msa1450440	0.812903	3.354668e-51	-8.615850e-47
Msa0278580	Msa1450440	0.803208	3.890853e-49	-8.615850e-47
Msa0348550	Msa1450440	0.810961	8.883629e-51	-8.615850e-47
Msa1122150	Msa1450440	0.833837	4.238468e-56	-8.615850e-47
Msa1140630	Msa1450440	0.833561	4.969257e-56	-8.615850e-47
Msa1160560	Msa1450440	0.842315	2.787675e-58	-8.615850e-47
Msa1175100	Msa1450440	0.815203	1.042761e-51	-8.615850e-47
Msa1181140	Msa1450440	0.822161	2.746268e-53	-8.615850e-47
Msa1213890	Msa1450440	0.824081	9.790492e-54	-8.615850e-47
Msa1214750	Msa1450440	0.812896	3.366431e-51	-8.615850e-47
Msa0526730	Msa1450440	0.807200	5.676095e-50	-8.615850e-47
Msa0534150	Msa1450440	0.806885	6.619680e-50	-8.615850e-47
Msa0545050	Msa1450440	0.837876	4.013218e-57	-8.615850e-47
Msa0556660	Msa1450440	0.830625	2.643342e-55	-8.615850e-47
Msa0558650	Msa1450440	0.823387	1.423996e-53	-8.615850e-47
Msa0567520	Msa1450440	0.816235	6.139143e-52	-8.615850e-47
Msa0574600	Msa1450440	0.813316	2.722591e-51	-8.615850e-47
Msa0576900	Msa1450440	0.815620	8.420112e-52	-8.615850e-47
Msa0600590	Msa1450440	0.827089	1.896806e-54	-8.615850e-47
Msa0857940	Msa1450440	0.819059	1.417075e-52	-8.615850e-47
Msa0878220	Msa1450440	0.855659	5.438930e-62	-8.615850e-47
Msa0899970	Msa1450440	0.802822	4.675363e-49	-8.615850e-47
Msa0943970	Msa1450440	0.858515	7.812003e-63	-8.615850e-47
Msa0944020	Msa1450440	0.847909	8.578119e-60	-8.615850e-47
Msa0965080	Msa1450440	0.817272	3.593872e-52	-8.615850e-47
Msa0965290	Msa1450440	0.833676	4.650179e-56	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450440	MtrunA17_Chr3g0125311	97.484	159	4	0	1	159	6	164	3.19e-107	302
Msa1450440	MtrunA17_Chr1g0160151	61.783	157	52	3	1	157	15	163	1.01e-55	172
Msa1450440	MtrunA17_Chr7g0245371	34.568	162	94	4	4	159	20	175	4.94e-22	87.0
Msa1450440	MtrunA17_Chr8g0351681	37.736	159	79	6	6	156	47	193	7.34e-19	79.3
Msa1450440	MtrunA17_Chr4g0033601	33.121	157	83	3	4	154	1	141	1.80e-18	77.0
Msa1450440	MtrunA17_Chr5g0402331	31.818	154	81	5	8	155	48	183	2.03e-17	75.1
Msa1450440	MtrunA17_Chr3g0111371	31.847	157	79	5	4	154	1	135	3.34e-17	73.6
Msa1450440	MtrunA17_Chr7g0256171	33.962	159	79	4	4	154	1	141	1.27e-16	72.4
Msa1450440	MtrunA17_Chr8g0391691	33.562	146	85	4	6	151	34	167	1.49e-16	73.2
Msa1450440	MtrunA17_Chr5g0402361	32.099	162	85	6	1	155	212	355	1.65e-16	75.1
Msa1450440	MtrunA17_Chr5g0402361	30.000	160	90	5	1	155	40	182	1.35e-14	69.7
Msa1450440	MtrunA17_Chr1g0161211	35.135	148	84	4	8	155	46	181	2.06e-16	72.8
Msa1450440	MtrunA17_Chr4g0064231	34.615	156	90	3	8	154	64	216	1.10e-15	71.2
Msa1450440	MtrunA17_Chr5g0444171	33.784	148	80	4	9	154	398	529	1.21e-15	73.2
Msa1450440	MtrunA17_Chr1g0169581	32.895	152	80	5	9	154	5	140	1.26e-15	69.3
Msa1450440	MtrunA17_Chr4g0057641	32.667	150	88	4	5	154	3	139	2.36e-15	68.6
Msa1450440	MtrunA17_Chr3g0121191	30.263	152	84	4	7	158	92	221	2.47e-15	70.5
Msa1450440	MtrunA17_Chr6g0482731	31.973	147	88	3	8	154	26	160	3.84e-15	68.9
Msa1450440	MtrunA17_Chr4g0074811	33.108	148	81	4	9	154	397	528	1.18e-14	70.5
Msa1450440	MtrunA17_Chr7g0245911	33.553	152	92	5	6	154	45	190	1.30e-14	68.2
Msa1450440	MtrunA17_Chr7g0225921	32.895	152	78	5	10	155	8	141	1.68e-14	66.6
Msa1450440	MtrunA17_Chr4g0057651	32.653	147	86	4	8	154	6	139	2.43e-14	66.2
Msa1450440	MtrunA17_Chr4g0057661	32.667	150	88	4	5	154	3	139	2.59e-14	65.9
Msa1450440	MtrunA17_Chr1g0188171	29.605	152	89	3	7	155	11	147	5.99e-14	65.1
Msa1450440	MtrunA17_Chr5g0434951	33.333	153	77	6	8	154	4	137	6.24e-14	65.1
Msa1450440	MtrunA17_Chr1g0166281	33.099	142	77	5	17	155	21	147	7.47e-14	65.1
Msa1450440	MtrunA17_Chr3g0098951	32.895	152	83	4	9	154	389	527	1.28e-13	67.4
Msa1450440	MtrunA17_Chr2g0329371	34.395	157	83	3	4	154	60	202	4.69e-13	63.9
Msa1450440	MtrunA17_Chr8g0392951	32.026	153	79	5	8	154	24	157	5.22e-13	63.2
Msa1450440	MtrunA17_Chr8g0387671	31.507	146	86	4	9	154	372	503	9.84e-13	64.7
Msa1450440	MtrunA17_Chr1g0176751	30.201	149	83	3	9	154	430	560	1.10e-12	64.7
Msa1450440	MtrunA17_Chr5g0441691	28.289	152	83	4	9	154	374	505	2.23e-12	63.5
Msa1450440	MtrunA17_Chr1g0185001	27.891	147	81	4	16	156	20	147	2.36e-12	60.8
Msa1450440	MtrunA17_Chr5g0440931	26.316	152	94	3	7	155	11	147	3.95e-12	60.5
Msa1450440	MtrunA17_Chr1g0200601	33.557	149	78	4	9	154	336	466	4.25e-12	62.8
Msa1450440	MtrunA17_Chr6g0460211	26.316	152	94	3	7	155	11	147	4.34e-12	60.5
Msa1450440	MtrunA17_Chr6g0459231	32.258	155	80	5	7	155	7	142	5.69e-12	59.7
Msa1450440	MtrunA17_Chr7g0254731	26.316	152	94	3	7	155	11	147	6.01e-12	60.1
Msa1450440	MtrunA17_Chr1g0157131	30.137	146	88	3	9	154	380	511	7.87e-12	62.0
Msa1450440	MtrunA17_Chr5g0397101	30.137	146	88	4	9	154	372	503	7.92e-12	62.0
Msa1450440	MtrunA17_Chr3g0093271	28.767	146	86	4	13	155	68	198	1.08e-11	60.1
Msa1450440	MtrunA17_Chr8g0366401	30.065	153	96	4	7	159	16	157	1.14e-11	59.7
Msa1450440	MtrunA17_Chr4g0071941	44.444	72	40	0	83	154	74	145	1.19e-11	59.3
Msa1450440	MtrunA17_Chr1g0169621	27.108	166	96	4	9	154	5	165	1.51e-11	59.3
Msa1450440	MtrunA17_Chr7g0217621	43.056	72	41	0	83	154	126	197	4.39e-11	58.5
Msa1450440	MtrunA17_Chr4g0044961	29.252	147	90	4	8	154	57	189	6.17e-11	58.2
Msa1450440	MtrunA17_Chr3g0116151	28.571	154	93	4	3	154	1	139	8.44e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450440	AT1G21550.1	41.935	155	84	3	2	155	4	153	1.81e-34	118
Msa1450440	AT4G20780.1	40.123	162	83	3	4	154	25	183	2.60e-26	99.0
Msa1450440	AT5G44460.1	38.037	163	87	4	4	159	24	179	2.65e-23	90.9
Msa1450440	AT1G66400.1	35.000	160	82	5	5	158	12	155	6.10e-21	84.0
Msa1450440	AT1G24620.1	36.364	154	80	5	8	158	37	175	1.17e-19	81.3
Msa1450440	AT2G43290.1	32.468	154	93	2	4	154	61	206	7.06e-19	80.1
Msa1450440	AT3G59440.1	34.615	156	79	3	5	154	48	186	1.24e-17	76.3
Msa1450440	AT1G05990.1	33.121	157	84	3	4	154	1	142	1.31e-17	75.1
Msa1450440	AT5G37770.1	33.121	157	83	4	5	154	14	155	1.65e-16	72.8
Msa1450440	AT5G37770.2	33.121	157	83	4	5	154	14	155	1.65e-16	72.8
Msa1450440	AT4G03290.1	33.121	157	86	3	4	154	1	144	8.82e-16	70.5
Msa1450440	AT4G04710.3	33.784	148	80	4	9	154	324	455	2.36e-15	72.8
Msa1450440	AT4G04710.1	33.784	148	80	4	9	154	351	482	2.55e-15	72.4
Msa1450440	AT4G04710.2	33.784	148	80	4	9	154	338	469	2.65e-15	72.4
Msa1450440	AT4G04740.4	32.877	146	84	3	9	154	374	505	2.90e-15	72.4
Msa1450440	AT4G04740.6	32.877	146	84	3	9	154	374	505	2.90e-15	72.4
Msa1450440	AT4G04740.5	32.877	146	84	3	9	154	374	505	2.90e-15	72.4
Msa1450440	AT4G04740.3	32.877	146	84	3	9	154	374	505	2.90e-15	72.4
Msa1450440	AT4G04740.1	32.877	146	84	3	9	154	374	505	2.90e-15	72.4
Msa1450440	AT4G04740.7	32.877	146	84	3	9	154	374	505	2.90e-15	72.4
Msa1450440	AT3G51850.1	33.758	157	71	5	8	154	359	492	4.85e-15	71.6
Msa1450440	AT4G04720.2	34.247	146	82	4	9	154	385	516	5.30e-15	71.6
Msa1450440	AT4G04720.1	34.247	146	82	4	9	154	385	516	5.30e-15	71.6
Msa1450440	AT1G76640.1	32.680	153	81	4	8	154	22	158	6.88e-15	68.2
Msa1450440	AT5G19360.1	29.530	149	85	3	9	154	374	505	8.71e-15	71.2
Msa1450440	AT5G12180.1	29.530	149	85	3	9	154	379	510	1.05e-14	70.9
Msa1450440	AT3G51920.1	30.137	146	84	4	16	158	20	150	1.48e-14	67.4
Msa1450440	AT3G07490.1	33.758	157	82	3	4	154	1	141	1.98e-14	67.0
Msa1450440	AT5G17470.1	30.405	148	89	5	9	154	3	138	2.25e-14	66.6
Msa1450440	AT4G04710.4	34.000	150	79	5	9	154	351	484	4.83e-14	68.9
Msa1450440	AT1G32250.1	31.293	147	95	2	8	154	16	156	8.46e-14	65.5
Msa1450440	AT1G73630.1	31.613	155	88	5	2	154	12	150	8.97e-14	65.5
Msa1450440	AT4G04700.1	34.932	146	81	4	9	154	337	468	1.11e-13	67.8
Msa1450440	AT4G04695.1	33.784	148	80	4	9	154	337	468	1.86e-13	67.4
Msa1450440	AT4G12860.1	29.221	154	93	2	4	154	1	141	2.09e-13	64.3
Msa1450440	AT1G35670.1	30.921	152	78	4	9	154	332	462	2.33e-13	67.0
Msa1450440	AT4G21940.2	32.192	146	85	4	9	154	407	538	2.91e-13	66.6
Msa1450440	AT4G21940.1	32.192	146	85	4	9	154	407	538	3.11e-13	66.6
Msa1450440	AT2G36180.1	29.677	155	82	5	9	154	1	137	4.77e-13	63.2
Msa1450440	AT5G42380.1	34.211	152	87	5	4	154	45	184	5.09e-13	63.9
Msa1450440	AT4G09570.1	31.579	152	77	4	9	154	331	461	2.02e-12	64.3
Msa1450440	AT1G66410.1	26.316	152	94	3	7	155	11	147	2.03e-12	61.6
Msa1450440	AT5G37780.1	26.316	152	94	3	7	155	11	147	2.03e-12	61.6
Msa1450440	AT4G23650.1	32.192	146	85	4	9	154	384	515	2.41e-12	63.9
Msa1450440	AT5G21274.1	26.974	152	93	3	7	155	11	147	2.76e-12	61.2
Msa1450440	AT1G18530.1	29.139	151	97	2	9	159	8	148	2.86e-12	61.2
Msa1450440	AT4G14640.1	28.188	149	95	2	7	155	12	148	2.87e-12	61.2
Msa1450440	AT3G47480.1	41.096	73	41	2	83	154	107	178	5.77e-12	61.2
Msa1450440	AT3G43810.1	26.316	152	94	3	7	155	11	147	5.99e-12	60.5
Msa1450440	AT3G43810.2	27.333	150	91	3	13	159	17	151	7.44e-12	61.2
Msa1450440	AT2G15680.1	29.333	150	92	4	5	154	47	182	1.08e-11	60.5
Msa1450440	AT2G38910.1	28.947	152	81	4	9	154	440	570	1.33e-11	62.0
Msa1450440	AT3G56800.1	25.658	152	95	3	7	155	11	147	1.82e-11	58.9
Msa1450440	AT2G41110.1	25.658	152	95	3	7	155	11	147	1.82e-11	58.9
Msa1450440	AT2G27030.1	25.658	152	95	3	7	155	11	147	1.82e-11	58.9
Msa1450440	AT1G50700.1	28.082	146	91	3	9	154	379	510	2.60e-11	61.2
Msa1450440	AT4G14640.2	28.358	134	84	2	22	155	9	130	3.48e-11	58.2
Msa1450440	AT1G18210.2	26.174	149	90	4	8	154	23	153	4.00e-11	58.5
Msa1450440	AT1G18210.1	26.174	149	90	4	8	154	23	153	4.00e-11	58.5
Msa1450440	AT2G27030.3	25.658	152	95	3	7	155	11	147	5.55e-11	58.5
Msa1450440	AT5G23580.1	29.530	149	84	3	9	154	328	458	6.31e-11	60.1
Msa1450440	AT5G37780.4	29.197	137	79	5	22	155	13	134	7.09e-11	57.4
Msa1450440	AT3G20410.2	28.082	146	91	3	9	154	397	528	7.67e-11	59.7
Msa1450440	AT3G20410.1	28.082	146	91	3	9	154	397	528	7.67e-11	59.7
Msa1450440	AT3G50770.1	29.299	157	94	5	5	156	61	205	7.71e-11	58.5

Find 21 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTATTATTGCATATTCTT+TGG	0.260505	tig0032904:-30415	Msa1450440:intergenic
TTCAAAGTGTTTGATTTAGA+TGG	0.287480	tig0032904:+30602	Msa1450440:CDS
GTTGAAGAGACTTGGATTCT+TGG	0.328203	tig0032904:+30661	Msa1450440:CDS
ATCAAGCCTACCATCCGAAT+TGG	0.406165	tig0032904:-30732	Msa1450440:intergenic
ACAAGAATTCATTGAAGTCT+AGG	0.438772	tig0032904:-30484	Msa1450440:intergenic
GGGATTGTAGAAGTATGATT+CGG	0.454094	tig0032904:+30699	Msa1450440:CDS
ATGATACCAATTCGGATGGT+AGG	0.471644	tig0032904:+30726	Msa1450440:CDS
TCAACACACACTCAAGCTCT+TGG	0.480809	tig0032904:-30643	Msa1450440:intergenic
GTGTTTGATTTAGATGGAGA+TGG	0.487484	tig0032904:+30608	Msa1450440:CDS
TGAGAATGATGAGTTGGAGA+GGG	0.503791	tig0032904:+30565	Msa1450440:CDS
GAGAACAATGAAGAGAATAG+AGG	0.509945	tig0032904:+30530	Msa1450440:CDS
CTTGGATGAAAGTAGTGGGA+GGG	0.521104	tig0032904:+30679	Msa1450440:CDS
ATGAGAATGATGAGTTGGAG+AGG	0.535268	tig0032904:+30564	Msa1450440:CDS
TGAAGATGAGAATGATGAGT+TGG	0.536288	tig0032904:+30559	Msa1450440:CDS
GAGTGTGTGTTGAAGAGACT+TGG	0.561588	tig0032904:+30653	Msa1450440:CDS
GGATTCTTGGATGAAAGTAG+TGG	0.564575	tig0032904:+30674	Msa1450440:CDS
AACAATGAAGAGAATAGAGG+AGG	0.573830	tig0032904:+30533	Msa1450440:CDS
GATTCTTGGATGAAAGTAGT+GGG	0.605078	tig0032904:+30675	Msa1450440:CDS
GCACAGAGTTTAGCTCCTCA+AGG	0.608778	tig0032904:-30391	Msa1450440:intergenic
AAGCTAGATACAAATTGTGA+CGG	0.623579	tig0032904:+30359	Msa1450440:CDS
TCTTGGATGAAAGTAGTGGG+AGG	0.636199	tig0032904:+30678	Msa1450440:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGCTATTATTGCATATTCTT+TGG	-	tig0032904:30418-30437	Msa1450440:intergenic	25.0%
!!	CTTTTACAACTCAATATCAA+AGG	+	tig0032904:30508-30527	Msa1450440:CDS	25.0%
!!	TTCAAAGTGTTTGATTTAGA+TGG	+	tig0032904:30602-30621	Msa1450440:CDS	25.0%
	AAGCTAGATACAAATTGTGA+CGG	+	tig0032904:30359-30378	Msa1450440:CDS	30.0%
	ACAAGAATTCATTGAAGTCT+AGG	-	tig0032904:30487-30506	Msa1450440:intergenic	30.0%
!!	TCGGTTTTATGATACCAATT+CGG	+	tig0032904:30718-30737	Msa1450440:CDS	30.0%
!	TTTTATGATACCAATTCGGA+TGG	+	tig0032904:30722-30741	Msa1450440:CDS	30.0%
	GAGAACAATGAAGAGAATAG+AGG	+	tig0032904:30530-30549	Msa1450440:CDS	35.0%
	AACAATGAAGAGAATAGAGG+AGG	+	tig0032904:30533-30552	Msa1450440:CDS	35.0%
	TGAAGATGAGAATGATGAGT+TGG	+	tig0032904:30559-30578	Msa1450440:CDS	35.0%
!	GTGTTTGATTTAGATGGAGA+TGG	+	tig0032904:30608-30627	Msa1450440:CDS	35.0%
	GATTCTTGGATGAAAGTAGT+GGG	+	tig0032904:30675-30694	Msa1450440:CDS	35.0%
!	GGGATTGTAGAAGTATGATT+CGG	+	tig0032904:30699-30718	Msa1450440:CDS	35.0%
!	ATGAGAATGATGAGTTGGAG+AGG	+	tig0032904:30564-30583	Msa1450440:CDS	40.0%
!	TGAGAATGATGAGTTGGAGA+GGG	+	tig0032904:30565-30584	Msa1450440:CDS	40.0%
	GTTGAAGAGACTTGGATTCT+TGG	+	tig0032904:30661-30680	Msa1450440:CDS	40.0%
	GGATTCTTGGATGAAAGTAG+TGG	+	tig0032904:30674-30693	Msa1450440:CDS	40.0%
	ATGATACCAATTCGGATGGT+AGG	+	tig0032904:30726-30745	Msa1450440:CDS	40.0%
	TCAACACACACTCAAGCTCT+TGG	-	tig0032904:30646-30665	Msa1450440:intergenic	45.0%
	GAGTGTGTGTTGAAGAGACT+TGG	+	tig0032904:30653-30672	Msa1450440:CDS	45.0%
!	TCTTGGATGAAAGTAGTGGG+AGG	+	tig0032904:30678-30697	Msa1450440:CDS	45.0%
!	CTTGGATGAAAGTAGTGGGA+GGG	+	tig0032904:30679-30698	Msa1450440:CDS	45.0%
	ATCAAGCCTACCATCCGAAT+TGG	-	tig0032904:30735-30754	Msa1450440:intergenic	45.0%
!	GGATGGTAGGCTTGATTTTC+AGG	+	tig0032904:30739-30758	Msa1450440:CDS	45.0%
!!	TGACGGTTTTGTGAGCCTTG+AGG	+	tig0032904:30376-30395	Msa1450440:CDS	50.0%
!	GCACAGAGTTTAGCTCCTCA+AGG	-	tig0032904:30394-30413	Msa1450440:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
tig0032904	gene	30317	30796	30317	ID=Msa1450440;Name=Msa1450440
tig0032904	mRNA	30317	30796	30317	ID=Msa1450440-mRNA-1;Parent=Msa1450440;Name=Msa1450440-mRNA-1;_AED=0.02;_eAED=0.07;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|159
tig0032904	exon	30317	30796	30317	ID=Msa1450440-mRNA-1:exon:17444;Parent=Msa1450440-mRNA-1
tig0032904	CDS	30317	30796	30317	ID=Msa1450440-mRNA-1:cds;Parent=Msa1450440-mRNA-1

Gene Sequence

>Msa1450440

ATGTCTATTCTATCTAGCACTGATTTGCATCGTATTTTTGAGAAGCTAGATACAAATTGTGACGGTTTTGTGAGCCTTGAGGAGCTAAACTCTGTGCTCCAAAGAATATGCAATAATAGCTCACAATTTAGCTTAGAAGAACTTGAATCTCTTGTAGAAAAGAAGAGCCTAGACTTCAATGAATTCTTGTTCTTTTACAACTCAATATCAAAGGAGAACAATGAAGAGAATAGAGGAGGTGATGAAGATGAGAATGATGAGTTGGAGAGGGATCTTGTGAAGACTTTCAAAGTGTTTGATTTAGATGGAGATGGATTCATTACAAGCCAAGAGCTTGAGTGTGTGTTGAAGAGACTTGGATTCTTGGATGAAAGTAGTGGGAGGGATTGTAGAAGTATGATTCGGTTTTATGATACCAATTCGGATGGTAGGCTTGATTTTCAGGAATTCAAGAACATGATGTTGATTACAAGTGTATGA

Protein sequence

>Msa1450440

MSILSSTDLHRIFEKLDTNCDGFVSLEELNSVLQRICNNSSQFSLEELESLVEKKSLDFNEFLFFYNSISKENNEENRGGDEDENDELERDLVKTFKVFDLDGDGFITSQELECVLKRLGFLDESSGRDCRSMIRFYDTNSDGRLDFQEFKNMMLITSV*