AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0840320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0840320 MtrunA17_Chr5g0409121 94.940 336 11 3 2 337 1 330 0.0 661
Msa0840320 MtrunA17_Chr4g0050421 73.828 256 67 0 82 337 90 345 7.41e-149 422
Msa0840320 MtrunA17_Chr5g0430001 67.045 264 87 0 74 337 60 323 8.20e-139 396
Msa0840320 MtrunA17_Chr5g0430011 66.300 273 89 1 68 337 52 324 2.87e-133 382
Msa0840320 MtrunA17_Chr2g0289361 26.667 180 111 3 151 310 109 287 2.16e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0840320 AT3G22370.1 79.421 311 61 1 30 337 44 354 0.0 519
Msa0840320 AT3G22360.1 84.249 273 41 1 67 337 53 325 2.31e-175 489
Msa0840320 AT3G27620.1 82.156 269 40 1 77 337 61 329 3.21e-168 471
Msa0840320 AT1G32350.1 70.182 275 74 1 71 337 44 318 4.56e-153 432
Msa0840320 AT5G64210.1 68.293 287 80 3 53 337 76 353 3.01e-147 419
Msa0840320 AT1G32350.2 70.312 256 68 1 71 318 44 299 8.08e-142 404

Find 97 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATACGGTGTGCAAATT+TGG 0.112313 5_4:+16015944 None:intergenic
TTCCTCAAAACTCTACTTTA+AGG 0.145682 5_4:-16015809 Msa0840320:CDS
ACCAATCTGTTGGCCTATTT+TGG 0.146207 5_4:-16015375 Msa0840320:three_prime_UTR
AAATTCTGAAGTCAATATTT+TGG 0.188734 5_4:-16015322 Msa0840320:three_prime_UTR
TTCTGGACAAGATGGCTTAT+TGG 0.228191 5_4:-16017761 Msa0840320:CDS
TTCTTCAATGCTTATTTCTT+AGG 0.253052 5_4:-16015981 Msa0840320:CDS
ATTGGACTGTTAAGAGTCTT+AGG 0.253076 5_4:-16017743 Msa0840320:CDS
ACAAAATAACCCCATTTGTT+TGG 0.260248 5_4:+16018150 None:intergenic
GGTGATAACAAAGATGAAAA+AGG 0.272034 5_4:-16018009 Msa0840320:CDS
CAGTTCACCACCACCAAAAT+AGG 0.299720 5_4:+16015362 None:intergenic
AAAAGGGATTACGAGTTATT+GGG 0.318703 5_4:-16017992 Msa0840320:CDS
TCCTCCGTCGTCGTCGTCTC+AGG 0.324683 5_4:-16018040 Msa0840320:CDS
TTTGCAAAGAAGGGTTTATT+AGG 0.324829 5_4:-16018189 Msa0840320:CDS
AATGGAAATGGAATTGCTTC+AGG 0.325465 5_4:-16017930 Msa0840320:CDS
CCACTTCCATGAAAGTCATT+AGG 0.338652 5_4:+16016054 None:intergenic
CGTAATGTTGTTTGCAAAGA+AGG 0.348388 5_4:-16018199 Msa0840320:CDS
ATGATGCCAATGTATAAAAC+AGG 0.353168 5_4:-16015236 Msa0840320:three_prime_UTR
CAGCTATTGCTATTGACTAT+TGG 0.356927 5_4:-16015836 Msa0840320:CDS
GGGATGTTGTCGAGGTTGTC+AGG 0.363968 5_4:-16015788 Msa0840320:CDS
CATGCACCTAATGACTTTCA+TGG 0.370912 5_4:-16016060 Msa0840320:CDS
ACCATACGGTGTGCAAATTT+GGG 0.381391 5_4:+16015945 None:intergenic
TCCTCAAAACTCTACTTTAA+GGG 0.382420 5_4:-16015808 Msa0840320:CDS
CTTCTTTGCAAACAACATTA+CGG 0.406181 5_4:+16018200 None:intergenic
TTAGAGAAAGAGAGAGAAAG+AGG 0.409678 5_4:+16018329 None:intergenic
GATCAACAACCCCTCTTGTA+AGG 0.418814 5_4:-16018125 Msa0840320:CDS
GGTTTATTAGGTGGTGAAGT+TGG 0.424159 5_4:-16018177 Msa0840320:CDS
AGGGAGGCTGCTGCTCCAAT+TGG 0.436968 5_4:-16015410 Msa0840320:CDS
AAGTTGGTGTTCCAAACAAA+TGG 0.440248 5_4:-16018161 Msa0840320:CDS
AGTTGGTGTTCCAAACAAAT+GGG 0.442514 5_4:-16018160 Msa0840320:CDS
AGCATTCCTCCTACCATGCC+AGG 0.449536 5_4:+16016164 None:intergenic
TCGTCGTCTCAGGGTGGTGC+TGG 0.451393 5_4:-16018030 Msa0840320:CDS
ATTACCAAGGACGAGAGTTA+AGG 0.452922 5_4:-16015430 Msa0840320:CDS
TTACCAAGGACGAGAGTTAA+GGG 0.457110 5_4:-16015429 Msa0840320:CDS
CTGATGGTACTGAATGGAAA+TGG 0.462655 5_4:-16017942 Msa0840320:CDS
TGCACTTGTCATAACTGTCC+AGG 0.463520 5_4:-16016009 Msa0840320:CDS
CATTTCCATTCAGTACCATC+AGG 0.464852 5_4:+16017943 None:intergenic
ATCTTGTCCAGAAAAGTTGT+TGG 0.465005 5_4:+16017771 None:intergenic
GGTATCTAGTGATAACCAAT+TGG 0.473206 5_4:+16015395 None:intergenic
GAGACAGTAGCTGCTGTCCC+TGG 0.491184 5_4:-16016182 Msa0840320:CDS
TCGATCCGGAACGACGAAAA+CGG 0.491381 5_4:+16018271 None:intergenic
ATCCACCACTGTGCTCAAAT+CGG 0.492142 5_4:+16016120 None:intergenic
CCTCCGTCGTCGTCGTCTCA+GGG 0.492164 5_4:-16018039 Msa0840320:CDS
CATCATCATCTTCTTCGATC+CGG 0.496661 5_4:+16018257 None:intergenic
GCTGCTGTCCCTGGCATGGT+AGG 0.504751 5_4:-16016173 Msa0840320:CDS
TCAATAGCAATAGCTGGAGC+AGG 0.506103 5_4:+16015843 None:intergenic
ACCCAAATTTGCACACCGTA+TGG 0.511597 5_4:-16015946 Msa0840320:CDS
CCTAATGACTTTCATGGAAG+TGG 0.513462 5_4:-16016054 Msa0840320:CDS
ATGGAAATGGAATTGCTTCA+GGG 0.515778 5_4:-16017929 Msa0840320:intron
TAATTGAAACAGAGAAGGTA+TGG 0.517621 5_4:-16016224 Msa0840320:intron
CAAAACCTGATGGTACTGAA+TGG 0.518207 5_4:-16017948 Msa0840320:CDS
ACCAAAATAGGCCAACAGAT+TGG 0.521985 5_4:+16015374 None:intergenic
TACTTTAAGGGATGTTGTCG+AGG 0.527952 5_4:-16015796 Msa0840320:CDS
CCTCCCTTAACTCTCGTCCT+TGG 0.532033 5_4:+16015426 None:intergenic
GTAATGTTGTTTGCAAAGAA+GGG 0.540746 5_4:-16018198 Msa0840320:CDS
GGATGTTGTCGAGGTTGTCA+GGG 0.547504 5_4:-16015787 Msa0840320:CDS
GCAAAGAAGGGTTTATTAGG+TGG 0.547710 5_4:-16018186 Msa0840320:CDS
AAAGGGATTACGAGTTATTG+GGG 0.549336 5_4:-16017991 Msa0840320:CDS
TGCTAATCTGTCAGATCAGA+AGG 0.555356 5_4:-16018085 Msa0840320:CDS
GCTGTCCCTGGCATGGTAGG+AGG 0.556200 5_4:-16016170 Msa0840320:CDS
ATCACTAGATACCAATCTGT+TGG 0.557864 5_4:-16015385 Msa0840320:exon
CTGTCTCAAGCATCATTGCT+CGG 0.558637 5_4:+16016198 None:intergenic
AGTGCACGCTCATACCACTT+TGG 0.562854 5_4:+16016026 None:intergenic
AAATTTGCACACCGTATGGT+TGG 0.563076 5_4:-16015942 Msa0840320:CDS
GTTTCTCAAGGAGCTTGACA+AGG 0.563279 5_4:-16015883 Msa0840320:CDS
CTCATACACTGAGTTTCTCA+AGG 0.566793 5_4:-16015895 Msa0840320:CDS
CAATAGTCAATAGCAATAGC+TGG 0.570433 5_4:+16015837 None:intergenic
TGCTTGCGTTCCTTACAAGA+GGG 0.572460 5_4:+16018115 None:intergenic
TCTGCTTTGTATGTCTCCCA+TGG 0.581364 5_4:+16017825 None:intergenic
CGCCGATTTGAGCACAGTGG+TGG 0.585049 5_4:-16016122 Msa0840320:CDS
GATTTGAGCACAGTGGTGGA+TGG 0.585457 5_4:-16016118 Msa0840320:CDS
GTGCTTGCGTTCCTTACAAG+AGG 0.587389 5_4:+16018114 None:intergenic
CGAAGAAGATGATGATGATG+AGG 0.590772 5_4:-16018251 Msa0840320:exon
GCACTTGTCATAACTGTCCA+GGG 0.592217 5_4:-16016008 Msa0840320:CDS
AGTAGCTGCTGTCCCTGGCA+TGG 0.598186 5_4:-16016177 Msa0840320:CDS
ATGATGAGGCATGGTGGTCA+CGG 0.609582 5_4:-16018237 Msa0840320:CDS
TCTAAGATCACAAAACCTGA+TGG 0.617644 5_4:-16017958 Msa0840320:CDS
CAATAGCAATAGCTGGAGCA+GGG 0.618562 5_4:+16015844 None:intergenic
CGATGTTAACCACTTTGCAT+CGG 0.619490 5_4:-16015745 Msa0840320:intron
CCACCCTGAGACGACGACGA+CGG 0.620652 5_4:+16018036 None:intergenic
CCTCGAGGTAACCAACCATA+CGG 0.624191 5_4:+16015931 None:intergenic
TGGAAGTGGCAAAGCCAAAG+TGG 0.631195 5_4:-16016040 Msa0840320:CDS
GCTTGCGTTCCTTACAAGAG+GGG 0.632754 5_4:+16018116 None:intergenic
GCATTCCTCCTACCATGCCA+GGG 0.651988 5_4:+16016165 None:intergenic
ACAAACATACCGATGCAAAG+TGG 0.652245 5_4:+16015736 None:intergenic
TTTCTCAAGGAGCTTGACAA+GGG 0.653346 5_4:-16015882 Msa0840320:CDS
CCCTGAGACGACGACGACGG+AGG 0.657783 5_4:+16018039 None:intergenic
GTTGGTGTTCCAAACAAATG+GGG 0.661779 5_4:-16018159 Msa0840320:CDS
TGATGCCAATGTATAAAACA+GGG 0.675711 5_4:-16015235 Msa0840320:three_prime_UTR
AAGATGATGATGATGAGGCA+TGG 0.678207 5_4:-16018246 Msa0840320:CDS
TGCCAATGTATAAAACAGGG+TGG 0.687062 5_4:-16015232 Msa0840320:three_prime_UTR
AGTGAATAGCTTCTTCCTCG+AGG 0.688820 5_4:+16015916 None:intergenic
TTGCAGGATATACATTACCA+AGG 0.701646 5_4:-16015443 Msa0840320:intron
CCGTCGTCGTCGTCTCAGGG+TGG 0.709337 5_4:-16018036 Msa0840320:CDS
CCGTATGGTTGGTTACCTCG+AGG 0.726979 5_4:-16015931 Msa0840320:CDS
ATGATGATGATGAGGCATGG+TGG 0.746370 5_4:-16018243 Msa0840320:CDS
CCAAGGACGAGAGTTAAGGG+AGG 0.747025 5_4:-16015426 Msa0840320:CDS
TTGCGCCGATTTGAGCACAG+TGG 0.750440 5_4:-16016125 Msa0840320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATTTTATTTATTTTA+TGG - chr5_4:16016539-16016558 Msa0840320:intron 0.0%
!!! AAAAATGATTTTTTTTAATG+TGG - chr5_4:16015678-16015697 Msa0840320:intron 10.0%
!! TCATTATTATTATTATTATC+AGG - chr5_4:16015887-16015906 Msa0840320:CDS 10.0%
!! CATTATTATTATTATTATCA+GGG - chr5_4:16015888-16015907 Msa0840320:CDS 10.0%
!!! GATTTTTATAATAATAATCA+GGG + chr5_4:16016350-16016369 None:intergenic 10.0%
!! TGATTATTATTATAAAAATC+CGG - chr5_4:16016350-16016369 Msa0840320:intron 10.0%
!!! TCAATTATATTGTTTTTTAA+AGG + chr5_4:16017007-16017026 None:intergenic 10.0%
!!! AGCAATTTTATTTATTTTTT+TGG + chr5_4:16018248-16018267 None:intergenic 10.0%
!!! GATTTTGTTTATTTTTCTTT+TGG - chr5_4:16016066-16016085 Msa0840320:CDS 15.0%
!!! GGATTTTTATAATAATAATC+AGG + chr5_4:16016351-16016370 None:intergenic 15.0%
!! GATTATTATTATAAAAATCC+GGG - chr5_4:16016351-16016370 Msa0840320:intron 15.0%
!!! TTTTTATTAATTTTCAAAGG+AGG + chr5_4:16016475-16016494 None:intergenic 15.0%
!!! ATTTTATTTATTTTATGGTC+AGG - chr5_4:16016544-16016563 Msa0840320:intron 15.0%
!!! TTTTATTTATTTTATGGTCA+GGG - chr5_4:16016545-16016564 Msa0840320:intron 15.0%
!! AAACCTAATTAAAAATATGA+TGG + chr5_4:16016728-16016747 None:intergenic 15.0%
!! AAATAAAAAATTGCTTAAAC+TGG - chr5_4:16017066-16017085 Msa0840320:intron 15.0%
!! TTCAAATTTCATTCTTATTA+GGG + chr5_4:16017170-16017189 None:intergenic 15.0%
!! ATTCAAATTTCATTCTTATT+AGG + chr5_4:16017171-16017190 None:intergenic 15.0%
!! AAACTTTGAACATAATTTAT+AGG + chr5_4:16017237-16017256 None:intergenic 15.0%
!!! ATTAGTATTAGTTGTTTTAT+TGG - chr5_4:16018275-16018294 Msa0840320:five_prime_UTR 15.0%
!!! TTTATTTTTCTTTTGGTGTA+TGG - chr5_4:16016073-16016092 Msa0840320:CDS 20.0%
!!! TTAATAAAAAAATGGATTCC+AGG - chr5_4:16016485-16016504 Msa0840320:intron 20.0%
!! TTATGCATGATTGAATATTT+AGG + chr5_4:16016762-16016781 None:intergenic 20.0%
!!! ACATAAAATACTTTTGATCT+TGG + chr5_4:16017276-16017295 None:intergenic 20.0%
!! GTAGCTTATAGATAAATAAA+TGG + chr5_4:16017864-16017883 None:intergenic 20.0%
!!! AAATTCTGAAGTCAATATTT+TGG - chr5_4:16018220-16018239 Msa0840320:CDS 20.0%
! GTGATAACAAAGATGAAAAA+GGG - chr5_4:16015534-16015553 Msa0840320:intron 25.0%
! ATTATTATTATTATCAGGGC+CGG - chr5_4:16015892-16015911 Msa0840320:CDS 25.0%
!!! ACTAGGCTGTAAATATTTTT+TGG + chr5_4:16015973-16015992 None:intergenic 25.0%
! CTGTTACAACAGATAAAATA+AGG + chr5_4:16016181-16016200 None:intergenic 25.0%
! CTCTTCTAATAAAGATTAGA+TGG + chr5_4:16016435-16016454 None:intergenic 25.0%
! CATCTAATCTTTATTAGAAG+AGG - chr5_4:16016433-16016452 Msa0840320:intron 25.0%
!! ATTAATTTTCAAAGGAGGTA+AGG + chr5_4:16016470-16016489 None:intergenic 25.0%
!!! TTTTAAGGTACTTTTGTTCA+AGG - chr5_4:16016699-16016718 Msa0840320:intron 25.0%
! CCTAATTAAAAATATGATGG+CGG + chr5_4:16016725-16016744 None:intergenic 25.0%
!! ATCCAATGTTTTATACTGTA+TGG - chr5_4:16016862-16016881 Msa0840320:intron 25.0%
! TACCATACAGTATAAAACAT+TGG + chr5_4:16016867-16016886 None:intergenic 25.0%
!!! GTGGATTTTTTCAAAACAAA+AGG + chr5_4:16016918-16016937 None:intergenic 25.0%
! GTATTTAATTGAAACAGAGA+AGG - chr5_4:16017313-16017332 Msa0840320:intron 25.0%
!! TTCTTCAATGCTTATTTCTT+AGG - chr5_4:16017561-16017580 Msa0840320:intron 25.0%
! CATAATAAAAAGACAGATCA+TGG + chr5_4:16017914-16017933 None:intergenic 25.0%
!! TTTCTAAAAAGACACAACTA+TGG + chr5_4:16018002-16018021 None:intergenic 25.0%
!!! TAGTTGTGTCTTTTTAGAAA+TGG - chr5_4:16018002-16018021 Msa0840320:CDS 25.0%
!!! TTCATCTGAGTATTTTGAAT+GGG - chr5_4:16018041-16018060 Msa0840320:CDS 25.0%
CTTCTTTGCAAACAACATTA+CGG + chr5_4:16015345-16015364 None:intergenic 30.0%
! GTAATGTTGTTTGCAAAGAA+GGG - chr5_4:16015344-16015363 Msa0840320:three_prime_UTR 30.0%
TTTGCAAAGAAGGGTTTATT+AGG - chr5_4:16015353-16015372 Msa0840320:three_prime_UTR 30.0%
ACAAAATAACCCCATTTGTT+TGG + chr5_4:16015395-16015414 None:intergenic 30.0%
GGTGATAACAAAGATGAAAA+AGG - chr5_4:16015533-16015552 Msa0840320:intron 30.0%
AAAAAGGGATTACGAGTTAT+TGG - chr5_4:16015549-16015568 Msa0840320:intron 30.0%
AAAAGGGATTACGAGTTATT+GGG - chr5_4:16015550-16015569 Msa0840320:intron 30.0%
! TCATAAACCAACAACTTTTC+TGG - chr5_4:16015764-16015783 Msa0840320:CDS 30.0%
!!! AGGCTGTAAATATTTTTTGG+AGG + chr5_4:16015970-16015989 None:intergenic 30.0%
!!! ATACAATGTGTTTTGGAAAG+GGG + chr5_4:16016275-16016294 None:intergenic 30.0%
!!! CATACAATGTGTTTTGGAAA+GGG + chr5_4:16016276-16016295 None:intergenic 30.0%
!!! TCATACAATGTGTTTTGGAA+AGG + chr5_4:16016277-16016296 None:intergenic 30.0%
! CTCATTCATACAATGTGTTT+TGG + chr5_4:16016282-16016301 None:intergenic 30.0%
TATTATAAAAATCCGGGTTC+TGG - chr5_4:16016357-16016376 Msa0840320:intron 30.0%
!! TTAAAACCTTTTCAGGAGTT+AGG + chr5_4:16016685-16016704 None:intergenic 30.0%
! TAACTCCTGAAAAGGTTTTA+AGG - chr5_4:16016684-16016703 Msa0840320:intron 30.0%
! AAGTACCTTAAAACCTTTTC+AGG + chr5_4:16016692-16016711 None:intergenic 30.0%
!! CCGCCATCATATTTTTAATT+AGG - chr5_4:16016722-16016741 Msa0840320:intron 30.0%
! GGATTTCTTTTCAAACCAAA+AGG + chr5_4:16016960-16016979 None:intergenic 30.0%
TAACAATAATAGCTAGCAAG+TGG + chr5_4:16016981-16017000 None:intergenic 30.0%
TAGGATAGTAAGTTCATCAA+GGG + chr5_4:16017218-16017237 None:intergenic 30.0%
ATAGGATAGTAAGTTCATCA+AGG + chr5_4:16017219-16017238 None:intergenic 30.0%
TAATTGAAACAGAGAAGGTA+TGG - chr5_4:16017318-16017337 Msa0840320:intron 30.0%
TTCCTCAAAACTCTACTTTA+AGG - chr5_4:16017733-16017752 Msa0840320:CDS 30.0%
TCCTCAAAACTCTACTTTAA+GGG - chr5_4:16017734-16017753 Msa0840320:CDS 30.0%
CTATAAGCTACAAATTCAAG+AGG - chr5_4:16017873-16017892 Msa0840320:intron 30.0%
CATTCAAAATACTCAGATGA+AGG + chr5_4:16018042-16018061 None:intergenic 30.0%
!! CTTCATCTGAGTATTTTGAA+TGG - chr5_4:16018040-16018059 Msa0840320:CDS 30.0%
! GTTTTCATGTAATGAATTGC+AGG - chr5_4:16018083-16018102 Msa0840320:CDS 30.0%
ATGATGCCAATGTATAAAAC+AGG - chr5_4:16018306-16018325 Msa0840320:five_prime_UTR 30.0%
TGATGCCAATGTATAAAACA+GGG - chr5_4:16018307-16018326 Msa0840320:five_prime_UTR 30.0%
TTAGAGAAAGAGAGAGAAAG+AGG + chr5_4:16015216-16015235 None:intergenic 35.0%
! CGTAATGTTGTTTGCAAAGA+AGG - chr5_4:16015343-16015362 Msa0840320:three_prime_UTR 35.0%
!! AAGTTGGTGTTCCAAACAAA+TGG - chr5_4:16015381-16015400 Msa0840320:exon 35.0%
! AGTTGGTGTTCCAAACAAAT+GGG - chr5_4:16015382-16015401 Msa0840320:exon 35.0%
AAAGGGATTACGAGTTATTG+GGG - chr5_4:16015551-16015570 Msa0840320:intron 35.0%
!! TCAGGTTTTGTGATCTTAGA+AGG + chr5_4:16015584-16015603 None:intergenic 35.0%
! TCTAAGATCACAAAACCTGA+TGG - chr5_4:16015584-16015603 Msa0840320:intron 35.0%
AATGGAAATGGAATTGCTTC+AGG - chr5_4:16015612-16015631 Msa0840320:intron 35.0%
ATGGAAATGGAATTGCTTCA+GGG - chr5_4:16015613-16015632 Msa0840320:intron 35.0%
!!! ATTTTGATTCTGACAGCCAT+GGG - chr5_4:16015701-16015720 Msa0840320:intron 35.0%
ATCTTGTCCAGAAAAGTTGT+TGG + chr5_4:16015774-16015793 None:intergenic 35.0%
! AACAACTTTTCTGGACAAGA+TGG - chr5_4:16015773-16015792 Msa0840320:CDS 35.0%
ATTGGACTGTTAAGAGTCTT+AGG - chr5_4:16015799-16015818 Msa0840320:CDS 35.0%
!!! TCCAACTGATCTCTTTTTTC+AGG - chr5_4:16015824-16015843 Msa0840320:CDS 35.0%
ACCTGAAAAAAGAGATCAGT+TGG + chr5_4:16015828-16015847 None:intergenic 35.0%
ATAGGAACTGGTATGTTAGT+GGG + chr5_4:16016000-16016019 None:intergenic 35.0%
!!! TGTTGCTGTTTCGGTATTTT+GGG - chr5_4:16016040-16016059 Msa0840320:CDS 35.0%
!! TCTTTTGGTGTATGGCTTTT+TGG - chr5_4:16016081-16016100 Msa0840320:CDS 35.0%
AAAGGACACAGCAGAAAAAA+AGG + chr5_4:16016125-16016144 None:intergenic 35.0%
TACTATACCAAAGCAAACAG+CGG + chr5_4:16016149-16016168 None:intergenic 35.0%
! AATGAGCCTCATTTTACTTC+CGG - chr5_4:16016296-16016315 Msa0840320:intron 35.0%
! AAATGGATTCCAGGAATTAC+TGG - chr5_4:16016494-16016513 Msa0840320:intron 35.0%
AAAATCACACCAGTAATTCC+TGG + chr5_4:16016506-16016525 None:intergenic 35.0%
TGCATAATGCATGTGATGTT+TGG - chr5_4:16016775-16016794 Msa0840320:intron 35.0%
GTCAGTTGGTTACATGAAAT+GGG - chr5_4:16016826-16016845 Msa0840320:intron 35.0%
AACAAAAGGTCATCATTCTG+TGG + chr5_4:16016904-16016923 None:intergenic 35.0%
TGAAAAAATCCACCACAAGA+TGG - chr5_4:16016925-16016944 Msa0840320:intron 35.0%
!! AGATGGTGCTTTTTACCTTT+TGG - chr5_4:16016942-16016961 Msa0840320:intron 35.0%
AGGATAGTAAGTTCATCAAG+GGG + chr5_4:16017217-16017236 None:intergenic 35.0%
!! AAGCATTGAAGAAAAAACCC+TGG + chr5_4:16017554-16017573 None:intergenic 35.0%
CAATAGTCAATAGCAATAGC+TGG + chr5_4:16017708-16017727 None:intergenic 35.0%
! CAGCTATTGCTATTGACTAT+TGG - chr5_4:16017706-16017725 Msa0840320:intron 35.0%
! TCCCTTAAAGTAGAGTTTTG+AGG + chr5_4:16017738-16017757 None:intergenic 35.0%
TTGCAGGATATACATTACCA+AGG - chr5_4:16018099-16018118 Msa0840320:CDS 35.0%
GGTATCTAGTGATAACCAAT+TGG + chr5_4:16018150-16018169 None:intergenic 35.0%
ATCACTAGATACCAATCTGT+TGG - chr5_4:16018157-16018176 Msa0840320:CDS 35.0%
! TTCCACCCTGTTTTATACAT+TGG + chr5_4:16018315-16018334 None:intergenic 35.0%
CATCATCATCTTCTTCGATC+CGG + chr5_4:16015288-16015307 None:intergenic 40.0%
! CGAAGAAGATGATGATGATG+AGG - chr5_4:16015291-16015310 Msa0840320:three_prime_UTR 40.0%
! AAGATGATGATGATGAGGCA+TGG - chr5_4:16015296-16015315 Msa0840320:three_prime_UTR 40.0%
GCAAAGAAGGGTTTATTAGG+TGG - chr5_4:16015356-16015375 Msa0840320:three_prime_UTR 40.0%
GGTTTATTAGGTGGTGAAGT+TGG - chr5_4:16015365-16015384 Msa0840320:three_prime_UTR 40.0%
! GTTGGTGTTCCAAACAAATG+GGG - chr5_4:16015383-16015402 Msa0840320:exon 40.0%
TGCTAATCTGTCAGATCAGA+AGG - chr5_4:16015457-16015476 Msa0840320:intron 40.0%
! CAAAACCTGATGGTACTGAA+TGG - chr5_4:16015594-16015613 Msa0840320:intron 40.0%
CATTTCCATTCAGTACCATC+AGG + chr5_4:16015602-16015621 None:intergenic 40.0%
! CTGATGGTACTGAATGGAAA+TGG - chr5_4:16015600-16015619 Msa0840320:intron 40.0%
!!! GATTTTGATTCTGACAGCCA+TGG - chr5_4:16015700-16015719 Msa0840320:intron 40.0%
TTCTGGACAAGATGGCTTAT+TGG - chr5_4:16015781-16015800 Msa0840320:CDS 40.0%
TAGGAACTGGTATGTTAGTG+GGG + chr5_4:16015999-16016018 None:intergenic 40.0%
CATAGGAACTGGTATGTTAG+TGG + chr5_4:16016001-16016020 None:intergenic 40.0%
AAAGCATGCTACAACAGCAT+AGG + chr5_4:16016018-16016037 None:intergenic 40.0%
!! ATGCTTTGCTGTTGCTGTTT+CGG - chr5_4:16016031-16016050 Msa0840320:CDS 40.0%
!!! CTGTTGCTGTTTCGGTATTT+TGG - chr5_4:16016039-16016058 Msa0840320:CDS 40.0%
! TTTGGTTTCTTTCCTGTTGC+TGG - chr5_4:16016099-16016118 Msa0840320:CDS 40.0%
GCTGTTTGCTTTGGTATAGT+AGG - chr5_4:16016148-16016167 Msa0840320:CDS 40.0%
TTTGCTTTGGTATAGTAGGC+TGG - chr5_4:16016152-16016171 Msa0840320:CDS 40.0%
TTGCTTTGGTATAGTAGGCT+GGG - chr5_4:16016153-16016172 Msa0840320:CDS 40.0%
TAGATGGTTCGCTACATTGT+GGG + chr5_4:16016419-16016438 None:intergenic 40.0%
TTAGATGGTTCGCTACATTG+TGG + chr5_4:16016420-16016439 None:intergenic 40.0%
GTTACACCTAACTCCTGAAA+AGG - chr5_4:16016676-16016695 Msa0840320:intron 40.0%
!! CATGTGATGTTTGGCTTGTT+TGG - chr5_4:16016784-16016803 Msa0840320:intron 40.0%
TTGTTTGGATGCATGTGCTT+TGG - chr5_4:16016799-16016818 Msa0840320:intron 40.0%
GGTCAGTTGGTTACATGAAA+TGG - chr5_4:16016825-16016844 Msa0840320:intron 40.0%
AAAAGGTCATCATTCTGTGG+TGG + chr5_4:16016901-16016920 None:intergenic 40.0%
!! TAAAAAGCACCATCTTGTGG+TGG + chr5_4:16016937-16016956 None:intergenic 40.0%
!! AGGTAAAAAGCACCATCTTG+TGG + chr5_4:16016940-16016959 None:intergenic 40.0%
CATGCACCTAATGACTTTCA+TGG - chr5_4:16017482-16017501 Msa0840320:intron 40.0%
CCACTTCCATGAAAGTCATT+AGG + chr5_4:16017491-16017510 None:intergenic 40.0%
CCTAATGACTTTCATGGAAG+TGG - chr5_4:16017488-16017507 Msa0840320:intron 40.0%
ACCATACGGTGTGCAAATTT+GGG + chr5_4:16017600-16017619 None:intergenic 40.0%
AACCATACGGTGTGCAAATT+TGG + chr5_4:16017601-16017620 None:intergenic 40.0%
AAATTTGCACACCGTATGGT+TGG - chr5_4:16017600-16017619 Msa0840320:intron 40.0%
CTCATACACTGAGTTTCTCA+AGG - chr5_4:16017647-16017666 Msa0840320:intron 40.0%
TTTCTCAAGGAGCTTGACAA+GGG - chr5_4:16017660-16017679 Msa0840320:intron 40.0%
! TACTTTAAGGGATGTTGTCG+AGG - chr5_4:16017746-16017765 Msa0840320:CDS 40.0%
CGATGTTAACCACTTTGCAT+CGG - chr5_4:16017797-16017816 Msa0840320:CDS 40.0%
ACAAACATACCGATGCAAAG+TGG + chr5_4:16017809-16017828 None:intergenic 40.0%
TTGCGCAAAAGTGAGACAAA+GGG + chr5_4:16017836-16017855 None:intergenic 40.0%
ATTGCGCAAAAGTGAGACAA+AGG + chr5_4:16017837-16017856 None:intergenic 40.0%
ATTACCAAGGACGAGAGTTA+AGG - chr5_4:16018112-16018131 Msa0840320:CDS 40.0%
TTACCAAGGACGAGAGTTAA+GGG - chr5_4:16018113-16018132 Msa0840320:CDS 40.0%
!! ACCAATCTGTTGGCCTATTT+TGG - chr5_4:16018167-16018186 Msa0840320:CDS 40.0%
ACCAAAATAGGCCAACAGAT+TGG + chr5_4:16018171-16018190 None:intergenic 40.0%
!! AATCTGTTGGCCTATTTTGG+TGG - chr5_4:16018170-16018189 Msa0840320:CDS 40.0%
TGCCAATGTATAAAACAGGG+TGG - chr5_4:16018310-16018329 Msa0840320:five_prime_UTR 40.0%
! ATGATGATGATGAGGCATGG+TGG - chr5_4:16015299-16015318 Msa0840320:three_prime_UTR 45.0%
GATCAACAACCCCTCTTGTA+AGG - chr5_4:16015417-16015436 Msa0840320:CDS 45.0%
TGCTTGCGTTCCTTACAAGA+GGG + chr5_4:16015430-16015449 None:intergenic 45.0%
TCTGCTTTGTATGTCTCCCA+TGG + chr5_4:16015720-16015739 None:intergenic 45.0%
!! TTTTTGGAGGCCCATACCTT+GGG + chr5_4:16015957-16015976 None:intergenic 45.0%
!!! TTTTTTGGAGGCCCATACCT+TGG + chr5_4:16015958-16015977 None:intergenic 45.0%
AGGAACTGGTATGTTAGTGG+GGG + chr5_4:16015998-16016017 None:intergenic 45.0%
TGCTACAACAGCATAGGAAC+TGG + chr5_4:16016012-16016031 None:intergenic 45.0%
!!! TGCTGTTTCGGTATTTTGGG+AGG - chr5_4:16016043-16016062 Msa0840320:CDS 45.0%
!! GCTGTTTCGGTATTTTGGGA+GGG - chr5_4:16016044-16016063 Msa0840320:CDS 45.0%
CAGAAAAAAAGGCCAGCAAC+AGG + chr5_4:16016114-16016133 None:intergenic 45.0%
! TTTGCCATTTAGTGCACTCC+TGG - chr5_4:16016201-16016220 Msa0840320:CDS 45.0%
! TATTTCAAGTGCCAAGCACC+AGG + chr5_4:16016222-16016241 None:intergenic 45.0%
TTCCAACGAATTTGGAGGCT+CGG + chr5_4:16016324-16016343 None:intergenic 45.0%
AGCCTCCAAATTCGTTGGAA+CGG - chr5_4:16016324-16016343 Msa0840320:intron 45.0%
TGAGAGTTGGATCTAGCAGA+AGG - chr5_4:16016385-16016404 Msa0840320:intron 45.0%
GAGAGTTGGATCTAGCAGAA+GGG - chr5_4:16016386-16016405 Msa0840320:intron 45.0%
TGGATGCATGTGCTTTGGTA+AGG - chr5_4:16016804-16016823 Msa0840320:intron 45.0%
TGTGCTTTGGTAAGGTCAGT+TGG - chr5_4:16016812-16016831 Msa0840320:intron 45.0%
CTGTCTCAAGCATCATTGCT+CGG + chr5_4:16017347-16017366 None:intergenic 45.0%
ATCCACCACTGTGCTCAAAT+CGG + chr5_4:16017425-16017444 None:intergenic 45.0%
TGCACTTGTCATAACTGTCC+AGG - chr5_4:16017533-16017552 Msa0840320:intron 45.0%
GCACTTGTCATAACTGTCCA+GGG - chr5_4:16017534-16017553 Msa0840320:intron 45.0%
ACCCAAATTTGCACACCGTA+TGG - chr5_4:16017596-16017615 Msa0840320:intron 45.0%
AGTGAATAGCTTCTTCCTCG+AGG + chr5_4:16017629-16017648 None:intergenic 45.0%
GTTTCTCAAGGAGCTTGACA+AGG - chr5_4:16017659-16017678 Msa0840320:intron 45.0%
CAATAGCAATAGCTGGAGCA+GGG + chr5_4:16017701-16017720 None:intergenic 45.0%
TCAATAGCAATAGCTGGAGC+AGG + chr5_4:16017702-16017721 None:intergenic 45.0%
CAGTTCACCACCACCAAAAT+AGG + chr5_4:16018183-16018202 None:intergenic 45.0%
!!! ATTTTTTTATTAATTTTCAA+AGG + chr5_4:16016478-16016497 None:intergenic 5.0%
!! TTTGAAAATTAATAAAAAAA+TGG - chr5_4:16016477-16016496 Msa0840320:intron 5.0%
!!! TCTTGCCGTTTTCGTCGTTC+CGG - chr5_4:16015266-16015285 Msa0840320:three_prime_UTR 50.0%
TCGATCCGGAACGACGAAAA+CGG + chr5_4:16015274-16015293 None:intergenic 50.0%
ATGATGAGGCATGGTGGTCA+CGG - chr5_4:16015305-16015324 Msa0840320:three_prime_UTR 50.0%
GCTTGCGTTCCTTACAAGAG+GGG + chr5_4:16015429-16015448 None:intergenic 50.0%
GTGCTTGCGTTCCTTACAAG+AGG + chr5_4:16015431-16015450 None:intergenic 50.0%
!! TTTTGGAGGCCCATACCTTG+GGG + chr5_4:16015956-16015975 None:intergenic 50.0%
!! GTATGTTAGTGGGGGGTACT+AGG + chr5_4:16015990-16016009 None:intergenic 50.0%
GGAACTGGTATGTTAGTGGG+GGG + chr5_4:16015997-16016016 None:intergenic 50.0%
TCCTTTGCCGCTGTTTGCTT+TGG - chr5_4:16016139-16016158 Msa0840320:CDS 50.0%
ACCAAAGCAAACAGCGGCAA+AGG + chr5_4:16016143-16016162 None:intergenic 50.0%
! AGCACCAGGAGTGCACTAAA+TGG + chr5_4:16016208-16016227 None:intergenic 50.0%
TTTAGTGCACTCCTGGTGCT+TGG - chr5_4:16016208-16016227 Msa0840320:CDS 50.0%
!! TCGGTGCCGGAAGTAAAATG+AGG + chr5_4:16016305-16016324 None:intergenic 50.0%
ACTCTCATCGATCCAGAACC+CGG + chr5_4:16016372-16016391 None:intergenic 50.0%
!! GGTTCTGGATCGATGAGAGT+TGG - chr5_4:16016372-16016391 Msa0840320:intron 50.0%
! GATTTGAGCACAGTGGTGGA+TGG - chr5_4:16017424-16017443 Msa0840320:intron 50.0%
TGGAAGTGGCAAAGCCAAAG+TGG - chr5_4:16017502-16017521 Msa0840320:intron 50.0%
AGTGCACGCTCATACCACTT+TGG + chr5_4:16017519-16017538 None:intergenic 50.0%
CCTCGAGGTAACCAACCATA+CGG + chr5_4:16017614-16017633 None:intergenic 50.0%
GGATGTTGTCGAGGTTGTCA+GGG - chr5_4:16017755-16017774 Msa0840320:CDS 50.0%
!!! CTGTTGGCCTATTTTGGTGG+TGG - chr5_4:16018173-16018192 Msa0840320:CDS 50.0%
TTATTATCAGGGCCGGACCC+TGG - chr5_4:16015899-16015918 Msa0840320:CDS 55.0%
TATTATCAGGGCCGGACCCT+GGG - chr5_4:16015900-16015919 Msa0840320:CDS 55.0%
CACCGAGCCTCCAAATTCGT+TGG - chr5_4:16016319-16016338 Msa0840320:intron 55.0%
GGCCGTTCCAACGAATTTGG+AGG + chr5_4:16016329-16016348 None:intergenic 55.0%
CAGGGCCGTTCCAACGAATT+TGG + chr5_4:16016332-16016351 None:intergenic 55.0%
GCATTCCTCCTACCATGCCA+GGG + chr5_4:16017380-16017399 None:intergenic 55.0%
AGCATTCCTCCTACCATGCC+AGG + chr5_4:16017381-16017400 None:intergenic 55.0%
!! TTGCGCCGATTTGAGCACAG+TGG - chr5_4:16017417-16017436 Msa0840320:intron 55.0%
! CCGTATGGTTGGTTACCTCG+AGG - chr5_4:16017611-16017630 Msa0840320:intron 55.0%
GGGATGTTGTCGAGGTTGTC+AGG - chr5_4:16017754-16017773 Msa0840320:CDS 55.0%
CCTCCCTTAACTCTCGTCCT+TGG + chr5_4:16018119-16018138 None:intergenic 55.0%
CCAAGGACGAGAGTTAAGGG+AGG - chr5_4:16018116-16018135 Msa0840320:CDS 55.0%
ATTATCAGGGCCGGACCCTG+GGG - chr5_4:16015901-16015920 Msa0840320:CDS 60.0%
GCAAGCAGCGCTATAGCACC+AGG - chr5_4:16015927-16015946 Msa0840320:CDS 60.0%
TATAGCACCAGGCCTCCCCA+AGG - chr5_4:16015938-16015957 Msa0840320:CDS 60.0%
ACCAGGCCTCCCCAAGGTAT+GGG - chr5_4:16015944-16015963 Msa0840320:CDS 60.0%
!! CGAATTTGGAGGCTCGGTGC+CGG + chr5_4:16016318-16016337 None:intergenic 60.0%
GAGACAGTAGCTGCTGTCCC+TGG - chr5_4:16017360-16017379 Msa0840320:intron 60.0%
AGTAGCTGCTGTCCCTGGCA+TGG - chr5_4:16017365-16017384 Msa0840320:intron 60.0%
!! CGCCGATTTGAGCACAGTGG+TGG - chr5_4:16017420-16017439 Msa0840320:intron 60.0%
AGGGAGGCTGCTGCTCCAAT+TGG - chr5_4:16018132-16018151 Msa0840320:CDS 60.0%
TCCTCCGTCGTCGTCGTCTC+AGG - chr5_4:16015502-16015521 Msa0840320:intron 65.0%
CCTCCGTCGTCGTCGTCTCA+GGG - chr5_4:16015503-16015522 Msa0840320:intron 65.0%
CCACCCTGAGACGACGACGA+CGG + chr5_4:16015509-16015528 None:intergenic 65.0%
TCGTCGTCTCAGGGTGGTGC+TGG - chr5_4:16015512-16015531 Msa0840320:intron 65.0%
TTATCAGGGCCGGACCCTGG+GGG - chr5_4:16015902-16015921 Msa0840320:CDS 65.0%
CACCAGGCCTCCCCAAGGTA+TGG - chr5_4:16015943-16015962 Msa0840320:CDS 65.0%
TGGAGGCCCATACCTTGGGG+AGG + chr5_4:16015953-16015972 None:intergenic 65.0%
GCTGCTGTCCCTGGCATGGT+AGG - chr5_4:16017369-16017388 Msa0840320:intron 65.0%
GCTGTCCCTGGCATGGTAGG+AGG - chr5_4:16017372-16017391 Msa0840320:intron 65.0%
CCCTGAGACGACGACGACGG+AGG + chr5_4:16015506-16015525 None:intergenic 70.0%
CCGTCGTCGTCGTCTCAGGG+TGG - chr5_4:16015506-16015525 Msa0840320:intron 70.0%
TATCAGGGCCGGACCCTGGG+GGG - chr5_4:16015903-16015922 Msa0840320:CDS 70.0%
TGCTTGCACCCCCCAGGGTC+CGG + chr5_4:16015914-16015933 None:intergenic 70.0%
AGCGCTGCTTGCACCCCCCA+GGG + chr5_4:16015919-16015938 None:intergenic 70.0%
TAGCGCTGCTTGCACCCCCC+AGG + chr5_4:16015920-16015939 None:intergenic 70.0%
GCCCATACCTTGGGGAGGCC+TGG + chr5_4:16015948-16015967 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 16015207 16018357 16015207 ID=Msa0840320;Name=Msa0840320
chr5_4 mRNA 16015207 16018357 16015207 ID=Msa0840320-mRNA-1;Parent=Msa0840320;Name=Msa0840320-mRNA-1;_AED=0.03;_eAED=0.03;_QI=92|1|1|1|1|1|4|193|337
chr5_4 exon 16015207 16015459 16015207 ID=Msa0840320-mRNA-1:exon:6889;Parent=Msa0840320-mRNA-1
chr5_4 exon 16015746 16016234 16015746 ID=Msa0840320-mRNA-1:exon:6888;Parent=Msa0840320-mRNA-1
chr5_4 exon 16017719 16017847 16017719 ID=Msa0840320-mRNA-1:exon:6887;Parent=Msa0840320-mRNA-1
chr5_4 exon 16017930 16018357 16017930 ID=Msa0840320-mRNA-1:exon:6886;Parent=Msa0840320-mRNA-1
chr5_4 five_prime_UTR 16018266 16018357 16018266 ID=Msa0840320-mRNA-1:five_prime_utr;Parent=Msa0840320-mRNA-1
chr5_4 CDS 16017930 16018265 16017930 ID=Msa0840320-mRNA-1:cds;Parent=Msa0840320-mRNA-1
chr5_4 CDS 16017719 16017847 16017719 ID=Msa0840320-mRNA-1:cds;Parent=Msa0840320-mRNA-1
chr5_4 CDS 16015746 16016234 16015746 ID=Msa0840320-mRNA-1:cds;Parent=Msa0840320-mRNA-1
chr5_4 CDS 16015400 16015459 16015400 ID=Msa0840320-mRNA-1:cds;Parent=Msa0840320-mRNA-1
chr5_4 three_prime_UTR 16015207 16015399 16015207 ID=Msa0840320-mRNA-1:three_prime_utr;Parent=Msa0840320-mRNA-1
Gene Sequence

>Msa0840320

ATGATGATGATGAGGCATGGTGGTCACGGTGCTGCTATGAACACCGTAATGTTGTTTGCAAAGAAGGGTTTATTAGGTGGTGAAGTTGGTGTTCCAAACAAATGGGGTTATTTTGTTAGATCAACAACCCCTCTTGTAAGGAACGCAAGCACTTTCACTGCTAATCTGTCAGATCAGAAGGACGACAAGACTGTCGACAAAACTCCTCCGTCGTCGTCGTCTCAGGGTGGTGCTGGTGATAACAAAGATGAAAAAGGGATTACGAGTTATTGGGGTGTTCAACCTTCTAAGATCACAAAACCTGATGGTACTGAATGGAAATGGAATTGCTTCAGGCCATGGGAGACATACAAAGCAGATGTTACGATTGATCTGACTAAACATCATAAACCAACAACTTTTCTGGACAAGATGGCTTATTGGACTGTTAAGAGTCTTAGGTATCCAACTGATCTCTTTTTTCAGAGAAGGTATGGATGCCGAGCAATGATGCTTGAGACAGTAGCTGCTGTCCCTGGCATGGTAGGAGGAATGCTGCTTCACTGCAAATCATTGCGCCGATTTGAGCACAGTGGTGGATGGATCAAAGCTTTGCTAGAAGAAGCAGAGAACGAGCGCATGCACCTAATGACTTTCATGGAAGTGGCAAAGCCAAAGTGGTATGAGCGTGCACTTGTCATAACTGTCCAGGGTTTTTTCTTCAATGCTTATTTCTTAGGCTATTTGCTCTCACCCAAATTTGCACACCGTATGGTTGGTTACCTCGAGGAAGAAGCTATTCACTCATACACTGAGTTTCTCAAGGAGCTTGACAAGGGTAATATTGAAAATGTCCCTGCTCCAGCTATTGCTATTGACTATTGGCAGCTTCCTCAAAACTCTACTTTAAGGGATGTTGTCGAGGTTGTCAGGGCTGATGAAGCACATCATCGCGATGTTAACCACTTTGCATCGGATATACATTACCAAGGACGAGAGTTAAGGGAGGCTGCTGCTCCAATTGGTTATCACTAG
Protein sequence

>Msa0840320

MMMMRHGGHGAAMNTVMLFAKKGLLGGEVGVPNKWGYFVRSTTPLVRNASTFTANLSDQKDDKTVDKTPPSSSSQGGAGDNKDEKGITSYWGVQPSKITKPDGTEWKWNCFRPWETYKADVTIDLTKHHKPTTFLDKMAYWTVKSLRYPTDLFFQRRYGCRAMMLETVAAVPGMVGGMLLHCKSLRRFEHSGGWIKALLEEAENERMHLMTFMEVAKPKWYERALVITVQGFFFNAYFLGYLLSPKFAHRMVGYLEEEAIHSYTEFLKELDKGNIENVPAPAIAIDYWQLPQNSTLRDVVEVVRADEAHHRDVNHFASDIHYQGRELREAAAPIGYH*