AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0760430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0760430 MtrunA17_Chr5g0409121 94.940 336 11 3 2 337 1 330 0.0 660
Msa0760430 MtrunA17_Chr4g0050421 75.600 250 61 0 88 337 96 345 7.96e-150 425
Msa0760430 MtrunA17_Chr5g0430001 67.424 264 86 0 74 337 60 323 7.82e-140 399
Msa0760430 MtrunA17_Chr5g0430011 64.085 284 92 1 54 337 51 324 2.72e-133 382
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0760430 AT3G22370.1 79.743 311 60 1 30 337 44 354 0.0 523
Msa0760430 AT3G22360.1 84.249 273 41 1 67 337 53 325 4.42e-176 491
Msa0760430 AT3G27620.1 82.528 269 39 1 77 337 61 329 5.82e-169 473
Msa0760430 AT1G32350.1 70.652 276 73 1 70 337 43 318 1.32e-154 436
Msa0760430 AT5G64210.1 70.438 274 75 2 66 337 84 353 5.02e-148 421
Msa0760430 AT1G32350.2 70.817 257 67 1 70 318 43 299 2.12e-143 407

Find 94 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATACGGTGTGCAAATT+TGG 0.112313 5_2:+16882531 None:intergenic
TTCCTCAAAACTCTACTTTA+AGG 0.145682 5_2:-16882396 Msa0760430:CDS
ACCAATCTGTTGGCCTATTT+TGG 0.146207 5_2:-16881963 Msa0760430:three_prime_UTR
AAATTCTGAAGTCAATATTT+TGG 0.188734 5_2:-16881910 Msa0760430:three_prime_UTR
TTCTGGACAAGATGGCTTAT+TGG 0.228191 5_2:-16883886 Msa0760430:CDS
TTCTTCAATGCTTATTTCTT+AGG 0.252741 5_2:-16882568 Msa0760430:CDS
ATTGGACTGTTAAGAGTCTT+AGG 0.253076 5_2:-16883868 Msa0760430:CDS
GGTGATAACAAAGATGAAAA+AGG 0.272034 5_2:-16884137 Msa0760430:CDS
ACGAAATAACCCCATTTGTT+TGG 0.275926 5_2:+16884278 None:intergenic
CAGTTCACCACCACCAAAAT+AGG 0.299720 5_2:+16881950 None:intergenic
AAAAGGGATTACGAGTTATT+GGG 0.318703 5_2:-16884120 Msa0760430:CDS
TTTGCAAAGAAGGGTTTATT+AGG 0.324829 5_2:-16884317 Msa0760430:CDS
AATGGAAATGGAATTGCTTC+AGG 0.325465 5_2:-16884058 Msa0760430:CDS
CCACTTCCATGAAAGTCATT+AGG 0.338652 5_2:+16882641 None:intergenic
CGTAATGTTGTTTGCAAAGA+AGG 0.348388 5_2:-16884327 Msa0760430:CDS
ATGATGCCAATGTATAAAAC+AGG 0.353168 5_2:-16881824 Msa0760430:three_prime_UTR
CAGCTATTGCTATTGACTAT+TGG 0.356927 5_2:-16882423 Msa0760430:CDS
GGGATGTTGTCGAGGTTGTC+AGG 0.363968 5_2:-16882375 Msa0760430:CDS
CATGCACCTAATGACTTTCA+TGG 0.370912 5_2:-16882647 Msa0760430:CDS
ACCATACGGTGTGCAAATTT+GGG 0.381391 5_2:+16882532 None:intergenic
TCCTCAAAACTCTACTTTAA+GGG 0.382420 5_2:-16882395 Msa0760430:CDS
CATCATCATCTTCTTCGTTC+CGG 0.393905 5_2:+16884385 None:intergenic
CTTCTTTGCAAACAACATTA+CGG 0.406181 5_2:+16884328 None:intergenic
TTAGAGAAAGAGAGAGAAAG+AGG 0.409678 5_2:+16884457 None:intergenic
GATCAACAACCCCTCTTGTA+AGG 0.418814 5_2:-16884253 Msa0760430:CDS
GGTTTATTAGGTGGTGAAGT+TGG 0.424159 5_2:-16884305 Msa0760430:CDS
AGGGAGGCTGCTGCTCCAAT+TGG 0.436968 5_2:-16881998 Msa0760430:CDS
AAGTTGGTGTTCCAAACAAA+TGG 0.440248 5_2:-16884289 Msa0760430:CDS
AGTTGGTGTTCCAAACAAAT+GGG 0.442514 5_2:-16884288 Msa0760430:CDS
AGCATTCCTCCTACCATGCC+AGG 0.449536 5_2:+16882751 None:intergenic
TCGTCGTCTCAGGGTGGTGC+TGG 0.451393 5_2:-16884158 Msa0760430:CDS
ATTACCAAGGACGAGAGTTA+AGG 0.452922 5_2:-16882018 Msa0760430:CDS
TTACCAAGGACGAGAGTTAA+GGG 0.457110 5_2:-16882017 Msa0760430:CDS
CTGATGGTACTGAATGGAAA+TGG 0.462655 5_2:-16884070 Msa0760430:CDS
TGCACTTGTCATAACTGTCC+AGG 0.463520 5_2:-16882596 Msa0760430:CDS
CATTTCCATTCAGTACCATC+AGG 0.464852 5_2:+16884071 None:intergenic
ATCTTGTCCAGAAAAGTTGT+TGG 0.465005 5_2:+16883896 None:intergenic
GGTATCTAGTGATAACCAAT+TGG 0.473206 5_2:+16881983 None:intergenic
GAGACAGTAGCTGCTGTCCC+TGG 0.491184 5_2:-16882769 Msa0760430:CDS
ATCCACCACTGTGCTCAAAT+CGG 0.492142 5_2:+16882707 None:intergenic
GCTGCTGTCCCTGGCATGGT+AGG 0.504751 5_2:-16882760 Msa0760430:CDS
TCAATAGCAATAGCTGGAGC+AGG 0.506103 5_2:+16882430 None:intergenic
ACCCAAATTTGCACACCGTA+TGG 0.511597 5_2:-16882533 Msa0760430:CDS
CCTAATGACTTTCATGGAAG+TGG 0.513462 5_2:-16882641 Msa0760430:CDS
ATGGAAATGGAATTGCTTCA+GGG 0.515778 5_2:-16884057 Msa0760430:intron
TAATTGAAACAGAGAAGGTA+TGG 0.517621 5_2:-16882811 Msa0760430:intron
CAAAACCTGATGGTACTGAA+TGG 0.518207 5_2:-16884076 Msa0760430:CDS
ACCAAAATAGGCCAACAGAT+TGG 0.521985 5_2:+16881962 None:intergenic
TACTTTAAGGGATGTTGTCG+AGG 0.527952 5_2:-16882383 Msa0760430:CDS
CCTCCCTTAACTCTCGTCCT+TGG 0.532033 5_2:+16882014 None:intergenic
GTAATGTTGTTTGCAAAGAA+GGG 0.540746 5_2:-16884326 Msa0760430:CDS
GGATGTTGTCGAGGTTGTCA+GGG 0.547504 5_2:-16882374 Msa0760430:CDS
GCAAAGAAGGGTTTATTAGG+TGG 0.547710 5_2:-16884314 Msa0760430:CDS
AAAGGGATTACGAGTTATTG+GGG 0.549336 5_2:-16884119 Msa0760430:CDS
TGCTAATCTGTCAGATCAGA+AGG 0.555356 5_2:-16884213 Msa0760430:CDS
GCTGTCCCTGGCATGGTAGG+AGG 0.556200 5_2:-16882757 Msa0760430:CDS
ATCACTAGATACCAATCTGT+TGG 0.557864 5_2:-16881973 Msa0760430:exon
CTGTCTCAAGCATCATTGCT+CGG 0.558637 5_2:+16882785 None:intergenic
AGTGCACGCTCATACCACTT+TGG 0.562854 5_2:+16882613 None:intergenic
AAATTTGCACACCGTATGGT+TGG 0.563076 5_2:-16882529 Msa0760430:CDS
GTTTCTCAAGGAGCTTGACA+AGG 0.563279 5_2:-16882470 Msa0760430:CDS
CTCATACACTGAGTTTCTCA+AGG 0.566793 5_2:-16882482 Msa0760430:CDS
CAATAGTCAATAGCAATAGC+TGG 0.570433 5_2:+16882424 None:intergenic
TGCTTGCGTTCCTTACAAGA+GGG 0.572460 5_2:+16884243 None:intergenic
TCTGCTTTGTATGTCTCCCA+TGG 0.581364 5_2:+16883950 None:intergenic
CGCCGATTTGAGCACAGTGG+TGG 0.585049 5_2:-16882709 Msa0760430:CDS
GATTTGAGCACAGTGGTGGA+TGG 0.585457 5_2:-16882705 Msa0760430:CDS
AGTGAATAGCTTCTTCCTCA+AGG 0.587319 5_2:+16882503 None:intergenic
GTGCTTGCGTTCCTTACAAG+AGG 0.587389 5_2:+16884242 None:intergenic
CGAAGAAGATGATGATGATG+AGG 0.587990 5_2:-16884379 Msa0760430:exon
AGTAGCTGCTGTCCCTGGCA+TGG 0.598186 5_2:-16882764 Msa0760430:CDS
ATGATGAGGCATGGTGGTCA+CGG 0.609582 5_2:-16884365 Msa0760430:CDS
TCTAAGATCACAAAACCTGA+TGG 0.617644 5_2:-16884086 Msa0760430:CDS
CAATAGCAATAGCTGGAGCA+GGG 0.618562 5_2:+16882431 None:intergenic
CGATGTTAACCACTTTGCAT+CGG 0.619490 5_2:-16882332 Msa0760430:intron
GCACTTGTCATAACTGTCCA+GGG 0.622780 5_2:-16882595 Msa0760430:CDS
CCTCAAGGTAACCAACCATA+CGG 0.624191 5_2:+16882518 None:intergenic
TGGAAGTGGCAAAGCCAAAG+TGG 0.631195 5_2:-16882627 Msa0760430:CDS
GCTTGCGTTCCTTACAAGAG+GGG 0.632754 5_2:+16884244 None:intergenic
GACAACATACCGATGCAAAG+TGG 0.644093 5_2:+16882323 None:intergenic
CCCTGAGACGACGACGACGA+CGG 0.644525 5_2:+16884167 None:intergenic
CCGTATGGTTGGTTACCTTG+AGG 0.649479 5_2:-16882518 Msa0760430:CDS
GCATTCCTCCTACCATGCCA+GGG 0.651988 5_2:+16882752 None:intergenic
TTTCTCAAGGAGCTTGACAA+GGG 0.653346 5_2:-16882469 Msa0760430:CDS
GTTGGTGTTCCAAACAAATG+GGG 0.661779 5_2:-16884287 Msa0760430:CDS
AAGCATTGAAGAAAACACCC+TGG 0.672042 5_2:+16882578 None:intergenic
TGATGCCAATGTATAAAACA+GGG 0.675711 5_2:-16881823 Msa0760430:three_prime_UTR
AAGATGATGATGATGAGGCA+TGG 0.678207 5_2:-16884374 Msa0760430:CDS
TGCCAATGTATAAAACAGGG+TGG 0.687062 5_2:-16881820 Msa0760430:three_prime_UTR
TTGCAGGATATACATTACCA+AGG 0.701646 5_2:-16882031 Msa0760430:intron
TCGTCGTCGTCGTCTCAGGG+TGG 0.724572 5_2:-16884164 Msa0760430:CDS
ATGATGATGATGAGGCATGG+TGG 0.746370 5_2:-16884371 Msa0760430:CDS
CCAAGGACGAGAGTTAAGGG+AGG 0.747025 5_2:-16882014 Msa0760430:CDS
TTGCGCCGATTTGAGCACAG+TGG 0.750440 5_2:-16882712 Msa0760430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATTTTATTTATTTTA+TGG - chr5_2:16882660-16882679 Msa0760430:CDS 0.0%
!!! AATGATTTTTTTTTTTAATG+TGG - chr5_2:16882269-16882288 Msa0760430:intron 10.0%
!! TATTATTATTATAAAAATCC+GGG - chr5_2:16882482-16882501 Msa0760430:CDS 10.0%
!!! TTTTTGTTAATTTTTTTCAA+AGG + chr5_2:16882604-16882623 None:intergenic 10.0%
!! GAAAAAAATTAACAAAAAAA+TGG - chr5_2:16882606-16882625 Msa0760430:CDS 10.0%
!!! ATTTTATTTATTTTATGGTC+AGG - chr5_2:16882665-16882684 Msa0760430:CDS 15.0%
!!! TTTTATTTATTTTATGGTCA+GGG - chr5_2:16882666-16882685 Msa0760430:CDS 15.0%
!! AAACCTAATTAAAAATATGA+TGG + chr5_2:16882857-16882876 None:intergenic 15.0%
!!! TCAATTATATTGTTTTTTAG+AGG + chr5_2:16883136-16883155 None:intergenic 15.0%
!! AAATAAAAAATTGCTTAAAC+TGG - chr5_2:16883195-16883214 Msa0760430:intron 15.0%
!! TTCAAATTTCATTCTTATTA+GGG + chr5_2:16883299-16883318 None:intergenic 15.0%
!! ATTCAAATTTCATTCTTATT+AGG + chr5_2:16883300-16883319 None:intergenic 15.0%
!! AAACTTTGAACATAATTTAT+AGG + chr5_2:16883366-16883385 None:intergenic 15.0%
!!! ATTAGTATTAGTTGTTTTAT+TGG - chr5_2:16884403-16884422 Msa0760430:five_prime_UTR 15.0%
!!! TTGTTAATTTTTTTCAAAGG+AGG + chr5_2:16882601-16882620 None:intergenic 20.0%
!!! TTAACAAAAAAATGGATTTC+AGG - chr5_2:16882614-16882633 Msa0760430:CDS 20.0%
!! TTATGCATGATTGAATATTT+AGG + chr5_2:16882891-16882910 None:intergenic 20.0%
!!! ACATAAAATACTTTTGATCT+TGG + chr5_2:16883405-16883424 None:intergenic 20.0%
!! GTAGCTTATAGATAAATAAA+TGG + chr5_2:16883992-16884011 None:intergenic 20.0%
!!! AAATTCTGAAGTCAATATTT+TGG - chr5_2:16884348-16884367 Msa0760430:CDS 20.0%
! GTGATAACAAAGATGAAAAA+GGG - chr5_2:16882122-16882141 Msa0760430:intron 25.0%
!!! TTTTAAGGTACTTTTGTTCA+AGG - chr5_2:16882828-16882847 Msa0760430:intron 25.0%
! CCTAATTAAAAATATGATGG+CGG + chr5_2:16882854-16882873 None:intergenic 25.0%
!! ATCCAATGTTTTATACTGTA+TGG - chr5_2:16882991-16883010 Msa0760430:intron 25.0%
! TACCATACAGTATAAAACAT+TGG + chr5_2:16882996-16883015 None:intergenic 25.0%
!!! GTGGATTTTTTCAAAACAAA+AGG + chr5_2:16883047-16883066 None:intergenic 25.0%
! GTATTTAATTGAAACAGAGA+AGG - chr5_2:16883442-16883461 Msa0760430:intron 25.0%
!! TTCTTCAATGCTTATTTCTT+AGG - chr5_2:16883690-16883709 Msa0760430:intron 25.0%
! CATAATAAAAAGACAGATCA+TGG + chr5_2:16884042-16884061 None:intergenic 25.0%
!! TTTCTAAAAAGACACAACTA+TGG + chr5_2:16884130-16884149 None:intergenic 25.0%
!!! TAGTTGTGTCTTTTTAGAAA+TGG - chr5_2:16884130-16884149 Msa0760430:CDS 25.0%
!!! TTCATCTGAGTATTTTGAAT+GGG - chr5_2:16884169-16884188 Msa0760430:CDS 25.0%
CTTCTTTGCAAACAACATTA+CGG + chr5_2:16881933-16881952 None:intergenic 30.0%
! GTAATGTTGTTTGCAAAGAA+GGG - chr5_2:16881932-16881951 Msa0760430:three_prime_UTR 30.0%
TTTGCAAAGAAGGGTTTATT+AGG - chr5_2:16881941-16881960 Msa0760430:three_prime_UTR 30.0%
GGTGATAACAAAGATGAAAA+AGG - chr5_2:16882121-16882140 Msa0760430:intron 30.0%
AAAAAGGGATTACGAGTTAT+TGG - chr5_2:16882137-16882156 Msa0760430:intron 30.0%
AAAAGGGATTACGAGTTATT+GGG - chr5_2:16882138-16882157 Msa0760430:intron 30.0%
! TCATAAACCAACAACTTTTC+TGG - chr5_2:16882355-16882374 Msa0760430:CDS 30.0%
TATTATAAAAATCCGGGTTC+TGG - chr5_2:16882488-16882507 Msa0760430:CDS 30.0%
CTCTTCTAATAATGATTCGA+TGG + chr5_2:16882566-16882585 None:intergenic 30.0%
CATCGAATCATTATTAGAAG+AGG - chr5_2:16882564-16882583 Msa0760430:CDS 30.0%
! AAATGGATTTCAGGAATTAC+TGG - chr5_2:16882623-16882642 Msa0760430:CDS 30.0%
!! CCGCCATCATATTTTTAATT+AGG - chr5_2:16882851-16882870 Msa0760430:intron 30.0%
! GGATTTCTTTTCAAACCAAA+AGG + chr5_2:16883089-16883108 None:intergenic 30.0%
TAACAATAATAGCTAGCAAG+TGG + chr5_2:16883110-16883129 None:intergenic 30.0%
TAGGATAGTAAGTTCATCAA+GGG + chr5_2:16883347-16883366 None:intergenic 30.0%
ATAGGATAGTAAGTTCATCA+AGG + chr5_2:16883348-16883367 None:intergenic 30.0%
TAATTGAAACAGAGAAGGTA+TGG - chr5_2:16883447-16883466 Msa0760430:intron 30.0%
TTCCTCAAAACTCTACTTTA+AGG - chr5_2:16883862-16883881 Msa0760430:CDS 30.0%
TCCTCAAAACTCTACTTTAA+GGG - chr5_2:16883863-16883882 Msa0760430:CDS 30.0%
CTATAAGCTACAAATTCAAG+AGG - chr5_2:16884001-16884020 Msa0760430:intron 30.0%
CATTCAAAATACTCAGATGA+AGG + chr5_2:16884170-16884189 None:intergenic 30.0%
!! CTTCATCTGAGTATTTTGAA+TGG - chr5_2:16884168-16884187 Msa0760430:CDS 30.0%
! GTTTTCATGTAATGAATTGC+AGG - chr5_2:16884211-16884230 Msa0760430:CDS 30.0%
ATGATGCCAATGTATAAAAC+AGG - chr5_2:16884434-16884453 Msa0760430:five_prime_UTR 30.0%
TGATGCCAATGTATAAAACA+GGG - chr5_2:16884435-16884454 Msa0760430:five_prime_UTR 30.0%
TTAGAGAAAGAGAGAGAAAG+AGG + chr5_2:16881804-16881823 None:intergenic 35.0%
! CGTAATGTTGTTTGCAAAGA+AGG - chr5_2:16881931-16881950 Msa0760430:three_prime_UTR 35.0%
!! AAGTTGGTGTTCCAAACAAA+TGG - chr5_2:16881969-16881988 Msa0760430:exon 35.0%
! AGTTGGTGTTCCAAACAAAT+GGG - chr5_2:16881970-16881989 Msa0760430:exon 35.0%
ACGAAATAACCCCATTTGTT+TGG + chr5_2:16881983-16882002 None:intergenic 35.0%
AAAGGGATTACGAGTTATTG+GGG - chr5_2:16882139-16882158 Msa0760430:intron 35.0%
!! TCAGGTTTTGTGATCTTAGA+AGG + chr5_2:16882172-16882191 None:intergenic 35.0%
! TCTAAGATCACAAAACCTGA+TGG - chr5_2:16882172-16882191 Msa0760430:intron 35.0%
AATGGAAATGGAATTGCTTC+AGG - chr5_2:16882200-16882219 Msa0760430:intron 35.0%
ATGGAAATGGAATTGCTTCA+GGG - chr5_2:16882201-16882220 Msa0760430:intron 35.0%
ATCTTGTCCAGAAAAGTTGT+TGG + chr5_2:16882365-16882384 None:intergenic 35.0%
! AACAACTTTTCTGGACAAGA+TGG - chr5_2:16882364-16882383 Msa0760430:CDS 35.0%
ATTGGACTGTTAAGAGTCTT+AGG - chr5_2:16882390-16882409 Msa0760430:CDS 35.0%
!!! TCCAACTGATCTCTTTTTTC+AGG - chr5_2:16882415-16882434 Msa0760430:CDS 35.0%
ACCTGAAAAAAGAGATCAGT+TGG + chr5_2:16882419-16882438 None:intergenic 35.0%
TTAAAACCTCTTCAGGAGTT+AGG + chr5_2:16882814-16882833 None:intergenic 35.0%
! TAACTCCTGAAGAGGTTTTA+AGG - chr5_2:16882813-16882832 Msa0760430:intron 35.0%
AAGTACCTTAAAACCTCTTC+AGG + chr5_2:16882821-16882840 None:intergenic 35.0%
TGCATAATGCATGTGATGTT+TGG - chr5_2:16882904-16882923 Msa0760430:intron 35.0%
GTCAGTTGGTTACATGAAAT+GGG - chr5_2:16882955-16882974 Msa0760430:intron 35.0%
AACAAAAGGTCATCATTCTG+TGG + chr5_2:16883033-16883052 None:intergenic 35.0%
TGAAAAAATCCACCACAAGA+TGG - chr5_2:16883054-16883073 Msa0760430:intron 35.0%
!! AGATGGTGCTTTTTACCTTT+TGG - chr5_2:16883071-16883090 Msa0760430:intron 35.0%
AGGATAGTAAGTTCATCAAG+GGG + chr5_2:16883346-16883365 None:intergenic 35.0%
CAATAGTCAATAGCAATAGC+TGG + chr5_2:16883837-16883856 None:intergenic 35.0%
! CAGCTATTGCTATTGACTAT+TGG - chr5_2:16883835-16883854 Msa0760430:intron 35.0%
! TCCCTTAAAGTAGAGTTTTG+AGG + chr5_2:16883867-16883886 None:intergenic 35.0%
TTGCAGGATATACATTACCA+AGG - chr5_2:16884227-16884246 Msa0760430:CDS 35.0%
GGTATCTAGTGATAACCAAT+TGG + chr5_2:16884278-16884297 None:intergenic 35.0%
ATCACTAGATACCAATCTGT+TGG - chr5_2:16884285-16884304 Msa0760430:CDS 35.0%
! TTCCACCCTGTTTTATACAT+TGG + chr5_2:16884443-16884462 None:intergenic 35.0%
CATCATCATCTTCTTCGTTC+CGG + chr5_2:16881876-16881895 None:intergenic 40.0%
! CGAAGAAGATGATGATGATG+AGG - chr5_2:16881879-16881898 Msa0760430:three_prime_UTR 40.0%
! AAGATGATGATGATGAGGCA+TGG - chr5_2:16881884-16881903 Msa0760430:three_prime_UTR 40.0%
GCAAAGAAGGGTTTATTAGG+TGG - chr5_2:16881944-16881963 Msa0760430:three_prime_UTR 40.0%
GGTTTATTAGGTGGTGAAGT+TGG - chr5_2:16881953-16881972 Msa0760430:three_prime_UTR 40.0%
! GTTGGTGTTCCAAACAAATG+GGG - chr5_2:16881971-16881990 Msa0760430:exon 40.0%
TGCTAATCTGTCAGATCAGA+AGG - chr5_2:16882045-16882064 Msa0760430:intron 40.0%
! CAAAACCTGATGGTACTGAA+TGG - chr5_2:16882182-16882201 Msa0760430:intron 40.0%
CATTTCCATTCAGTACCATC+AGG + chr5_2:16882190-16882209 None:intergenic 40.0%
! CTGATGGTACTGAATGGAAA+TGG - chr5_2:16882188-16882207 Msa0760430:intron 40.0%
!!! ATTTTGATTGCGACAGCCAT+GGG - chr5_2:16882292-16882311 Msa0760430:intron 40.0%
TTCTGGACAAGATGGCTTAT+TGG - chr5_2:16882372-16882391 Msa0760430:CDS 40.0%
!! CATGTGATGTTTGGCTTGTT+TGG - chr5_2:16882913-16882932 Msa0760430:intron 40.0%
GGTCAGTTGGTTACATGAAA+TGG - chr5_2:16882954-16882973 Msa0760430:intron 40.0%
AAAAGGTCATCATTCTGTGG+TGG + chr5_2:16883030-16883049 None:intergenic 40.0%
!! TAAAAAGCACCATCTTGTGG+TGG + chr5_2:16883066-16883085 None:intergenic 40.0%
!! AGGTAAAAAGCACCATCTTG+TGG + chr5_2:16883069-16883088 None:intergenic 40.0%
CATGCACCTAATGACTTTCA+TGG - chr5_2:16883611-16883630 Msa0760430:intron 40.0%
CCACTTCCATGAAAGTCATT+AGG + chr5_2:16883620-16883639 None:intergenic 40.0%
CCTAATGACTTTCATGGAAG+TGG - chr5_2:16883617-16883636 Msa0760430:intron 40.0%
!! AAGCATTGAAGAAAACACCC+TGG + chr5_2:16883683-16883702 None:intergenic 40.0%
ACCATACGGTGTGCAAATTT+GGG + chr5_2:16883729-16883748 None:intergenic 40.0%
AACCATACGGTGTGCAAATT+TGG + chr5_2:16883730-16883749 None:intergenic 40.0%
AAATTTGCACACCGTATGGT+TGG - chr5_2:16883729-16883748 Msa0760430:intron 40.0%
AGTGAATAGCTTCTTCCTCA+AGG + chr5_2:16883758-16883777 None:intergenic 40.0%
CTCATACACTGAGTTTCTCA+AGG - chr5_2:16883776-16883795 Msa0760430:intron 40.0%
TTTCTCAAGGAGCTTGACAA+GGG - chr5_2:16883789-16883808 Msa0760430:intron 40.0%
! TACTTTAAGGGATGTTGTCG+AGG - chr5_2:16883875-16883894 Msa0760430:CDS 40.0%
CGATGTTAACCACTTTGCAT+CGG - chr5_2:16883926-16883945 Msa0760430:CDS 40.0%
ATTACCAAGGACGAGAGTTA+AGG - chr5_2:16884240-16884259 Msa0760430:CDS 40.0%
TTACCAAGGACGAGAGTTAA+GGG - chr5_2:16884241-16884260 Msa0760430:CDS 40.0%
!! ACCAATCTGTTGGCCTATTT+TGG - chr5_2:16884295-16884314 Msa0760430:CDS 40.0%
ACCAAAATAGGCCAACAGAT+TGG + chr5_2:16884299-16884318 None:intergenic 40.0%
!! AATCTGTTGGCCTATTTTGG+TGG - chr5_2:16884298-16884317 Msa0760430:CDS 40.0%
TGCCAATGTATAAAACAGGG+TGG - chr5_2:16884438-16884457 Msa0760430:five_prime_UTR 40.0%
! ATGATGATGATGAGGCATGG+TGG - chr5_2:16881887-16881906 Msa0760430:three_prime_UTR 45.0%
GATCAACAACCCCTCTTGTA+AGG - chr5_2:16882005-16882024 Msa0760430:CDS 45.0%
TGCTTGCGTTCCTTACAAGA+GGG + chr5_2:16882018-16882037 None:intergenic 45.0%
!!! GATTTTGATTGCGACAGCCA+TGG - chr5_2:16882291-16882310 Msa0760430:intron 45.0%
TCTGCTTTGTATGTCTCCCA+TGG + chr5_2:16882311-16882330 None:intergenic 45.0%
TGAGAGTTGGATCTAGCTGA+AGG - chr5_2:16882516-16882535 Msa0760430:CDS 45.0%
GAGAGTTGGATCTAGCTGAA+GGG - chr5_2:16882517-16882536 Msa0760430:CDS 45.0%
GGTTAGACTCCCACAATGTA+GGG - chr5_2:16882538-16882557 Msa0760430:CDS 45.0%
TCGATGGTTCCCTACATTGT+GGG + chr5_2:16882550-16882569 None:intergenic 45.0%
TTCGATGGTTCCCTACATTG+TGG + chr5_2:16882551-16882570 None:intergenic 45.0%
TTCAGGAGTTAGGTGGAGTT+AGG + chr5_2:16882804-16882823 None:intergenic 45.0%
AAACCTCTTCAGGAGTTAGG+TGG + chr5_2:16882811-16882830 None:intergenic 45.0%
TTGTTTGGATGCACGTGCTT+TGG - chr5_2:16882928-16882947 Msa0760430:intron 45.0%
CTGTCTCAAGCATCATTGCT+CGG + chr5_2:16883476-16883495 None:intergenic 45.0%
ATCCACCACTGTGCTCAAAT+CGG + chr5_2:16883554-16883573 None:intergenic 45.0%
TGCACTTGTCATAACTGTCC+AGG - chr5_2:16883662-16883681 Msa0760430:intron 45.0%
GCACTTGTCATAACTGTCCA+GGG - chr5_2:16883663-16883682 Msa0760430:intron 45.0%
ACCCAAATTTGCACACCGTA+TGG - chr5_2:16883725-16883744 Msa0760430:intron 45.0%
CCTCAAGGTAACCAACCATA+CGG + chr5_2:16883743-16883762 None:intergenic 45.0%
GTTTCTCAAGGAGCTTGACA+AGG - chr5_2:16883788-16883807 Msa0760430:intron 45.0%
CAATAGCAATAGCTGGAGCA+GGG + chr5_2:16883830-16883849 None:intergenic 45.0%
TCAATAGCAATAGCTGGAGC+AGG + chr5_2:16883831-16883850 None:intergenic 45.0%
GACAACATACCGATGCAAAG+TGG + chr5_2:16883938-16883957 None:intergenic 45.0%
TTGCGCAAGAGTGAGACAAA+GGG + chr5_2:16883964-16883983 None:intergenic 45.0%
ATTGCGCAAGAGTGAGACAA+AGG + chr5_2:16883965-16883984 None:intergenic 45.0%
CAGTTCACCACCACCAAAAT+AGG + chr5_2:16884311-16884330 None:intergenic 45.0%
!! TTATTATTATTATAAAAATC+CGG - chr5_2:16882481-16882500 Msa0760430:CDS 5.0%
!!! TCTTGCCGTTTTCGTCGTTC+CGG - chr5_2:16881854-16881873 Msa0760430:three_prime_UTR 50.0%
TCGTTCCGGAACGACGAAAA+CGG + chr5_2:16881862-16881881 None:intergenic 50.0%
ATGATGAGGCATGGTGGTCA+CGG - chr5_2:16881893-16881912 Msa0760430:three_prime_UTR 50.0%
GCTTGCGTTCCTTACAAGAG+GGG + chr5_2:16882017-16882036 None:intergenic 50.0%
GTGCTTGCGTTCCTTACAAG+AGG + chr5_2:16882019-16882038 None:intergenic 50.0%
ACTCTCATCGATCCAGAACC+CGG + chr5_2:16882503-16882522 None:intergenic 50.0%
!! GGTTCTGGATCGATGAGAGT+TGG - chr5_2:16882503-16882522 Msa0760430:CDS 50.0%
GGGTTAGACTCCCACAATGT+AGG - chr5_2:16882537-16882556 Msa0760430:CDS 50.0%
ACTCCACCTAACTCCTGAAG+AGG - chr5_2:16882805-16882824 Msa0760430:intron 50.0%
TGGATGCACGTGCTTTGGTA+AGG - chr5_2:16882933-16882952 Msa0760430:intron 50.0%
CGTGCTTTGGTAAGGTCAGT+TGG - chr5_2:16882941-16882960 Msa0760430:intron 50.0%
! GATTTGAGCACAGTGGTGGA+TGG - chr5_2:16883553-16883572 Msa0760430:intron 50.0%
TGGAAGTGGCAAAGCCAAAG+TGG - chr5_2:16883631-16883650 Msa0760430:intron 50.0%
AGTGCACGCTCATACCACTT+TGG + chr5_2:16883648-16883667 None:intergenic 50.0%
! CCGTATGGTTGGTTACCTTG+AGG - chr5_2:16883740-16883759 Msa0760430:intron 50.0%
GGATGTTGTCGAGGTTGTCA+GGG - chr5_2:16883884-16883903 Msa0760430:CDS 50.0%
!!! CTGTTGGCCTATTTTGGTGG+TGG - chr5_2:16884301-16884320 Msa0760430:CDS 50.0%
GCATTCCTCCTACCATGCCA+GGG + chr5_2:16883509-16883528 None:intergenic 55.0%
AGCATTCCTCCTACCATGCC+AGG + chr5_2:16883510-16883529 None:intergenic 55.0%
!! TTGCGCCGATTTGAGCACAG+TGG - chr5_2:16883546-16883565 Msa0760430:intron 55.0%
GGGATGTTGTCGAGGTTGTC+AGG - chr5_2:16883883-16883902 Msa0760430:CDS 55.0%
CCTCCCTTAACTCTCGTCCT+TGG + chr5_2:16884247-16884266 None:intergenic 55.0%
CCAAGGACGAGAGTTAAGGG+AGG - chr5_2:16884244-16884263 Msa0760430:CDS 55.0%
GAGACAGTAGCTGCTGTCCC+TGG - chr5_2:16883489-16883508 Msa0760430:intron 60.0%
AGTAGCTGCTGTCCCTGGCA+TGG - chr5_2:16883494-16883513 Msa0760430:intron 60.0%
!! CGCCGATTTGAGCACAGTGG+TGG - chr5_2:16883549-16883568 Msa0760430:intron 60.0%
AGGGAGGCTGCTGCTCCAAT+TGG - chr5_2:16884260-16884279 Msa0760430:CDS 60.0%
TCCGTCGTCGTCGTCGTCTC+AGG - chr5_2:16882090-16882109 Msa0760430:intron 65.0%
CCCTGAGACGACGACGACGA+CGG + chr5_2:16882094-16882113 None:intergenic 65.0%
CCGTCGTCGTCGTCGTCTCA+GGG - chr5_2:16882091-16882110 Msa0760430:intron 65.0%
TCGTCGTCGTCGTCTCAGGG+TGG - chr5_2:16882094-16882113 Msa0760430:intron 65.0%
TCGTCGTCTCAGGGTGGTGC+TGG - chr5_2:16882100-16882119 Msa0760430:intron 65.0%
GCTGCTGTCCCTGGCATGGT+AGG - chr5_2:16883498-16883517 Msa0760430:intron 65.0%
GCTGTCCCTGGCATGGTAGG+AGG - chr5_2:16883501-16883520 Msa0760430:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 16881795 16884485 16881795 ID=Msa0760430;Name=Msa0760430
chr5_2 mRNA 16881795 16884485 16881795 ID=Msa0760430-mRNA-1;Parent=Msa0760430;Name=Msa0760430-mRNA-1;_AED=0.04;_eAED=0.04;_QI=92|1|1|1|1|1|4|193|337
chr5_2 exon 16881795 16882047 16881795 ID=Msa0760430-mRNA-1:exon:7473;Parent=Msa0760430-mRNA-1
chr5_2 exon 16882333 16882821 16882333 ID=Msa0760430-mRNA-1:exon:7472;Parent=Msa0760430-mRNA-1
chr5_2 exon 16883844 16883972 16883844 ID=Msa0760430-mRNA-1:exon:7471;Parent=Msa0760430-mRNA-1
chr5_2 exon 16884058 16884485 16884058 ID=Msa0760430-mRNA-1:exon:7470;Parent=Msa0760430-mRNA-1
chr5_2 five_prime_UTR 16884394 16884485 16884394 ID=Msa0760430-mRNA-1:five_prime_utr;Parent=Msa0760430-mRNA-1
chr5_2 CDS 16884058 16884393 16884058 ID=Msa0760430-mRNA-1:cds;Parent=Msa0760430-mRNA-1
chr5_2 CDS 16883844 16883972 16883844 ID=Msa0760430-mRNA-1:cds;Parent=Msa0760430-mRNA-1
chr5_2 CDS 16882333 16882821 16882333 ID=Msa0760430-mRNA-1:cds;Parent=Msa0760430-mRNA-1
chr5_2 CDS 16881988 16882047 16881988 ID=Msa0760430-mRNA-1:cds;Parent=Msa0760430-mRNA-1
chr5_2 three_prime_UTR 16881795 16881987 16881795 ID=Msa0760430-mRNA-1:three_prime_utr;Parent=Msa0760430-mRNA-1
Gene Sequence

>Msa0760430

ATGATGATGATGAGGCATGGTGGTCACGGTGCTGCTATGAACACCGTAATGTTGTTTGCAAAGAAGGGTTTATTAGGTGGTGAAGTTGGTGTTCCAAACAAATGGGGTTATTTCGTTAGATCAACAACCCCTCTTGTAAGGAACGCAAGCACTTTCACTGCTAATCTGTCAGATCAGAAGGACGACAAGACTGTCGACAAAACTCCGTCGTCGTCGTCGTCTCAGGGTGGTGCTGGTGATAACAAAGATGAAAAAGGGATTACGAGTTATTGGGGTGTTCAACCTTCTAAGATCACAAAACCTGATGGTACTGAATGGAAATGGAATTGCTTCAGGCCATGGGAGACATACAAAGCAGATGTTACGATTGATCTGACTAAACATCATAAACCAACAACTTTTCTGGACAAGATGGCTTATTGGACTGTTAAGAGTCTTAGGTATCCAACTGATCTCTTTTTTCAGAGAAGGTATGGATGCCGAGCAATGATGCTTGAGACAGTAGCTGCTGTCCCTGGCATGGTAGGAGGAATGCTGCTTCACTGCAAATCATTGCGCCGATTTGAGCACAGTGGTGGATGGATCAAAGCTTTGCTAGAAGAAGCAGAGAACGAGCGCATGCACCTAATGACTTTCATGGAAGTGGCAAAGCCAAAGTGGTATGAGCGTGCACTTGTCATAACTGTCCAGGGTGTTTTCTTCAATGCTTATTTCTTAGGCTATTTGCTCTCACCCAAATTTGCACACCGTATGGTTGGTTACCTTGAGGAAGAAGCTATTCACTCATACACTGAGTTTCTCAAGGAGCTTGACAAGGGTAATATTGAAAATGTCCCTGCTCCAGCTATTGCTATTGACTATTGGCAGCTTCCTCAAAACTCTACTTTAAGGGATGTTGTCGAGGTTGTCAGGGCTGATGAAGCACATCATCGCGATGTTAACCACTTTGCATCGGATATACATTACCAAGGACGAGAGTTAAGGGAGGCTGCTGCTCCAATTGGTTATCACTAG
Protein sequence

>Msa0760430

MMMMRHGGHGAAMNTVMLFAKKGLLGGEVGVPNKWGYFVRSTTPLVRNASTFTANLSDQKDDKTVDKTPSSSSSQGGAGDNKDEKGITSYWGVQPSKITKPDGTEWKWNCFRPWETYKADVTIDLTKHHKPTTFLDKMAYWTVKSLRYPTDLFFQRRYGCRAMMLETVAAVPGMVGGMLLHCKSLRRFEHSGGWIKALLEEAENERMHLMTFMEVAKPKWYERALVITVQGVFFNAYFLGYLLSPKFAHRMVGYLEEEAIHSYTEFLKELDKGNIENVPAPAIAIDYWQLPQNSTLRDVVEVVRADEAHHRDVNHFASDIHYQGRELREAAAPIGYH*