AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0841660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0841660 MtrunA17_Chr5g0411231 95.000 120 6 0 20 139 1 120 6.80e-81 233
Msa0841660 MtrunA17_Chr5g0411241 85.000 120 18 0 20 139 1 120 3.89e-70 206
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTAATTTGTACTAAATT+TGG 0.168371 5_4:-18148984 Msa0841660:three_prime_UTR
ATTCAAAGACTTATCAAAAT+AGG 0.190071 5_4:+18148654 None:intergenic
TCACTTCTCTAGGGACAATT+TGG 0.245955 5_4:+18149045 None:intergenic
CTTGCTCTTGAGTGCAGCTT+TGG 0.250449 5_4:+18150056 None:intergenic
TTCCCTCTATCATGAATAAT+TGG 0.255625 5_4:+18150194 None:intergenic
TCACTGCTACTCTCAATTTC+AGG 0.257468 5_4:+18149207 None:intergenic
CTCATATCATTGTGAGAATA+TGG 0.263715 5_4:-18150083 Msa0841660:CDS
TTCAAAGACTTATCAAAATA+GGG 0.331440 5_4:+18148655 None:intergenic
TTGCTCTTGAGTGCAGCTTT+GGG 0.361214 5_4:+18150057 None:intergenic
TGTGTGATCAGCTATGAATT+TGG 0.395112 5_4:-18148945 Msa0841660:three_prime_UTR
GGTCCAATTATTCATGATAG+AGG 0.415239 5_4:-18150197 Msa0841660:five_prime_UTR
GTTTGTAATTAGTATGTGTT+TGG 0.438400 5_4:-18148912 Msa0841660:three_prime_UTR
GGCAGTGTGTTGTGCAAGAC+TGG 0.455435 5_4:-18149836 Msa0841660:CDS
CCCTTGCCTGAGTCTGCATC+AGG 0.461443 5_4:+18149117 None:intergenic
GTAATGCAATAGTATGTGTT+TGG 0.464999 5_4:-18148874 Msa0841660:three_prime_UTR
TCAGACTGTTCTTATAATTG+TGG 0.466472 5_4:+18148763 None:intergenic
CCTTAGTGAATCACTTCTCT+AGG 0.478513 5_4:+18149035 None:intergenic
TTCAAGCTAATAAAATATCA+AGG 0.479076 5_4:-18149008 Msa0841660:three_prime_UTR
TACTAAATTTGGTTTATCAT+CGG 0.484979 5_4:-18148973 Msa0841660:three_prime_UTR
AGGTTCAATTATTGAGAAAG+AGG 0.485478 5_4:-18150240 Msa0841660:five_prime_UTR
TGTGTTATAACTAGGACTAC+TGG 0.487878 5_4:-18149800 Msa0841660:CDS
ATTAAGAAATTACAGATCAC+TGG 0.494783 5_4:-18148704 Msa0841660:three_prime_UTR
GAGGTAGAGCCTTGAAAAGT+CGG 0.524080 5_4:+18150168 None:intergenic
CCTAGAGAAGTGATTCACTA+AGG 0.524929 5_4:-18149035 Msa0841660:three_prime_UTR
AATGTATCAACTAGTGAATG+AGG 0.527069 5_4:+18150149 None:intergenic
ATAAGCTTTGTGCAGGAAAG+TGG 0.528788 5_4:-18149891 Msa0841660:intron
AGGACTACTGGACCTGATGC+TGG 0.536873 5_4:-18149788 Msa0841660:CDS
CAAAGCTGCACTCAAGAGCA+AGG 0.561178 5_4:-18150055 Msa0841660:CDS
AGAAATTACAGATCACTGGT+TGG 0.565069 5_4:-18148700 Msa0841660:three_prime_UTR
TCCTGATGCAGACTCAGGCA+AGG 0.574927 5_4:-18149118 Msa0841660:CDS
GTCCAATTATTCATGATAGA+GGG 0.575626 5_4:-18150196 Msa0841660:five_prime_UTR
GAAGGTCCTGATGCAGACTC+AGG 0.576340 5_4:-18149123 Msa0841660:CDS
CTAGAGAAGTGATTCACTAA+GGG 0.578373 5_4:-18149034 Msa0841660:three_prime_UTR
GTTGGACATGTGTTATAACT+AGG 0.581816 5_4:-18149808 Msa0841660:CDS
TCTGCAAGTGTGGTGAAGGA+TGG 0.591787 5_4:-18149167 Msa0841660:CDS
ACATTCTGCAAGTGTGGTGA+AGG 0.593456 5_4:-18149171 Msa0841660:CDS
CTTAGTGAATCACTTCTCTA+GGG 0.599847 5_4:+18149036 None:intergenic
ACAACACACTGCCCAAACCC+AGG 0.603905 5_4:+18149846 None:intergenic
TCTTGTGCATGCAGAGATGG+TGG 0.610106 5_4:-18149240 Msa0841660:intron
GCTGTTTATGACTTTAAACA+TGG 0.619952 5_4:-18150218 Msa0841660:five_prime_UTR
ATTGAGAGTAGCAGTGACAA+TGG 0.620823 5_4:-18149201 Msa0841660:CDS
GTGTGTGACATACAGTGTGC+AGG 0.624270 5_4:+18149733 None:intergenic
GTGTTGTGCAAGACTGGTAA+AGG 0.626364 5_4:-18149830 Msa0841660:CDS
TATAAGAACAGTCTGAAGCA+TGG 0.629013 5_4:-18148756 Msa0841660:three_prime_UTR
CCTGATGCAGACTCAGGCAA+GGG 0.633802 5_4:-18149117 Msa0841660:CDS
AAAGCTGCACTCAAGAGCAA+GGG 0.647133 5_4:-18150054 Msa0841660:intron
TGTGCAAGACTGGTAAAGGT+TGG 0.662647 5_4:-18149826 Msa0841660:CDS
AGTGAAACATTCTGCAAGTG+TGG 0.671634 5_4:-18149177 Msa0841660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTAATTTGTACTAAATT+TGG - chr5_4:18149947-18149966 Msa0841660:intron 10.0%
!!! TAATTAATTTGTACTAAATT+TGG - chr5_4:18149947-18149966 Msa0841660:intron 10.0%
!! AAAACTATATAGATGAAATA+AGG + chr5_4:18148930-18148949 None:intergenic 15.0%
!! GATTTATTTATGTATGAAAA+TGG + chr5_4:18149008-18149027 None:intergenic 15.0%
!!! AAAGTATTATAGTTCATTTT+TGG - chr5_4:18149662-18149681 Msa0841660:intron 15.0%
!! AAAACTATATAGATGAAATA+AGG + chr5_4:18148930-18148949 None:intergenic 15.0%
!! GATTTATTTATGTATGAAAA+TGG + chr5_4:18149008-18149027 None:intergenic 15.0%
!!! AAAGTATTATAGTTCATTTT+TGG - chr5_4:18149662-18149681 Msa0841660:intron 15.0%
!! GTAAAATTAAAATAGGCAAA+AGG + chr5_4:18149228-18149247 None:intergenic 20.0%
!! GATGGTAGTAAAATTAAAAT+AGG + chr5_4:18149235-18149254 None:intergenic 20.0%
!!! TTTTTCAAAATCGAATGAAT+TGG - chr5_4:18149302-18149321 Msa0841660:intron 20.0%
!!! AGTTAATTCTGAAAAACAAT+AGG + chr5_4:18149556-18149575 None:intergenic 20.0%
!! ATTAACTCTTGAGATAAAAT+CGG - chr5_4:18149569-18149588 Msa0841660:intron 20.0%
!! TTCAAGCTAATAAAATATCA+AGG - chr5_4:18149923-18149942 Msa0841660:intron 20.0%
!!! TACTAAATTTGGTTTATCAT+CGG - chr5_4:18149958-18149977 Msa0841660:intron 20.0%
!! GTAAAATTAAAATAGGCAAA+AGG + chr5_4:18149228-18149247 None:intergenic 20.0%
!! GATGGTAGTAAAATTAAAAT+AGG + chr5_4:18149235-18149254 None:intergenic 20.0%
!!! TTTTTCAAAATCGAATGAAT+TGG - chr5_4:18149302-18149321 Msa0841660:intron 20.0%
!!! AGTTAATTCTGAAAAACAAT+AGG + chr5_4:18149556-18149575 None:intergenic 20.0%
!! ATTAACTCTTGAGATAAAAT+CGG - chr5_4:18149569-18149588 Msa0841660:intron 20.0%
!! TTCAAGCTAATAAAATATCA+AGG - chr5_4:18149923-18149942 Msa0841660:intron 20.0%
!!! TACTAAATTTGGTTTATCAT+CGG - chr5_4:18149958-18149977 Msa0841660:intron 20.0%
! TTTGATCAAAATCATTTCGA+TGG + chr5_4:18149253-18149272 None:intergenic 25.0%
! AATGAAACTGCAATGTAATA+AGG + chr5_4:18149378-18149397 None:intergenic 25.0%
! TGTTGTAACAATAGTTGAAT+CGG + chr5_4:18149507-18149526 None:intergenic 25.0%
! ATTATATAAGCCTAGATTTG+TGG + chr5_4:18149620-18149639 None:intergenic 25.0%
! GTTTGTAATTAGTATGTGTT+TGG - chr5_4:18150019-18150038 Msa0841660:intron 25.0%
! ATTAAGAAATTACAGATCAC+TGG - chr5_4:18150227-18150246 Msa0841660:five_prime_UTR 25.0%
! TTTGATCAAAATCATTTCGA+TGG + chr5_4:18149253-18149272 None:intergenic 25.0%
! AATGAAACTGCAATGTAATA+AGG + chr5_4:18149378-18149397 None:intergenic 25.0%
! TGTTGTAACAATAGTTGAAT+CGG + chr5_4:18149507-18149526 None:intergenic 25.0%
! ATTATATAAGCCTAGATTTG+TGG + chr5_4:18149620-18149639 None:intergenic 25.0%
! GTTTGTAATTAGTATGTGTT+TGG - chr5_4:18150019-18150038 Msa0841660:intron 25.0%
! ATTAAGAAATTACAGATCAC+TGG - chr5_4:18150227-18150246 Msa0841660:five_prime_UTR 25.0%
!! GAAGCAGTATTTTTCAATCT+AGG - chr5_4:18148671-18148690 Msa0841660:three_prime_UTR 30.0%
AGGTTCAATTATTGAGAAAG+AGG - chr5_4:18148691-18148710 Msa0841660:three_prime_UTR 30.0%
GCTGTTTATGACTTTAAACA+TGG - chr5_4:18148713-18148732 Msa0841660:three_prime_UTR 30.0%
GTCCAATTATTCATGATAGA+GGG - chr5_4:18148735-18148754 Msa0841660:three_prime_UTR 30.0%
TTCCCTCTATCATGAATAAT+TGG + chr5_4:18148740-18148759 None:intergenic 30.0%
AATGTATCAACTAGTGAATG+AGG + chr5_4:18148785-18148804 None:intergenic 30.0%
CTCATATCATTGTGAGAATA+TGG - chr5_4:18148848-18148867 Msa0841660:three_prime_UTR 30.0%
AAATGGAACAAGAGTAGTAT+GGG + chr5_4:18148991-18149010 None:intergenic 30.0%
AAAATGGAACAAGAGTAGTA+TGG + chr5_4:18148992-18149011 None:intergenic 30.0%
! GGCAAAGTTTTTGTTAAATG+CGG - chr5_4:18149164-18149183 Msa0841660:CDS 30.0%
TCAAAATCGAATGAATTGGT+TGG - chr5_4:18149306-18149325 Msa0841660:intron 30.0%
AGATTTGTGGCATATTTGAA+TGG + chr5_4:18149607-18149626 None:intergenic 30.0%
TTCAAATATGCCACAAATCT+AGG - chr5_4:18149607-18149626 Msa0841660:intron 30.0%
GTAATGCAATAGTATGTGTT+TGG - chr5_4:18150057-18150076 Msa0841660:CDS 30.0%
!! GAAATTTTAAGAGACAACAG+AGG + chr5_4:18150109-18150128 None:intergenic 30.0%
TCAGACTGTTCTTATAATTG+TGG + chr5_4:18150171-18150190 None:intergenic 30.0%
!! GAAGCAGTATTTTTCAATCT+AGG - chr5_4:18148671-18148690 Msa0841660:three_prime_UTR 30.0%
AGGTTCAATTATTGAGAAAG+AGG - chr5_4:18148691-18148710 Msa0841660:three_prime_UTR 30.0%
GCTGTTTATGACTTTAAACA+TGG - chr5_4:18148713-18148732 Msa0841660:three_prime_UTR 30.0%
GTCCAATTATTCATGATAGA+GGG - chr5_4:18148735-18148754 Msa0841660:three_prime_UTR 30.0%
TTCCCTCTATCATGAATAAT+TGG + chr5_4:18148740-18148759 None:intergenic 30.0%
AATGTATCAACTAGTGAATG+AGG + chr5_4:18148785-18148804 None:intergenic 30.0%
CTCATATCATTGTGAGAATA+TGG - chr5_4:18148848-18148867 Msa0841660:three_prime_UTR 30.0%
AAATGGAACAAGAGTAGTAT+GGG + chr5_4:18148991-18149010 None:intergenic 30.0%
AAAATGGAACAAGAGTAGTA+TGG + chr5_4:18148992-18149011 None:intergenic 30.0%
! GGCAAAGTTTTTGTTAAATG+CGG - chr5_4:18149164-18149183 Msa0841660:CDS 30.0%
TCAAAATCGAATGAATTGGT+TGG - chr5_4:18149306-18149325 Msa0841660:intron 30.0%
AGATTTGTGGCATATTTGAA+TGG + chr5_4:18149607-18149626 None:intergenic 30.0%
TTCAAATATGCCACAAATCT+AGG - chr5_4:18149607-18149626 Msa0841660:intron 30.0%
GTAATGCAATAGTATGTGTT+TGG - chr5_4:18150057-18150076 Msa0841660:CDS 30.0%
!! GAAATTTTAAGAGACAACAG+AGG + chr5_4:18150109-18150128 None:intergenic 30.0%
TCAGACTGTTCTTATAATTG+TGG + chr5_4:18150171-18150190 None:intergenic 30.0%
GGTCCAATTATTCATGATAG+AGG - chr5_4:18148734-18148753 Msa0841660:three_prime_UTR 35.0%
! TAAACCAATAAGCTTTGTGC+AGG - chr5_4:18149033-18149052 Msa0841660:three_prime_UTR 35.0%
GTAGTTCAAGACAAAAACTC+AGG - chr5_4:18149062-18149081 Msa0841660:exon 35.0%
GTTGGACATGTGTTATAACT+AGG - chr5_4:18149123-18149142 Msa0841660:CDS 35.0%
TGTGTTATAACTAGGACTAC+TGG - chr5_4:18149131-18149150 Msa0841660:CDS 35.0%
! AATGAATTGGTTGGTTCAAC+TGG - chr5_4:18149315-18149334 Msa0841660:intron 35.0%
AATAGGACTCTGGATCAAAT+CGG + chr5_4:18149539-18149558 None:intergenic 35.0%
TCTGAAAAACAATAGGACTC+TGG + chr5_4:18149549-18149568 None:intergenic 35.0%
!! GATGGAGTTGTTCCATTTTT+AGG - chr5_4:18149782-18149801 Msa0841660:CDS 35.0%
!!! TGTTCCATTTTTAGGACTGA+AGG - chr5_4:18149790-18149809 Msa0841660:CDS 35.0%
CTTAGTGAATCACTTCTCTA+GGG + chr5_4:18149898-18149917 None:intergenic 35.0%
CTAGAGAAGTGATTCACTAA+GGG - chr5_4:18149897-18149916 Msa0841660:intron 35.0%
TGTGTGATCAGCTATGAATT+TGG - chr5_4:18149986-18150005 Msa0841660:intron 35.0%
! TATAAGAACAGTCTGAAGCA+TGG - chr5_4:18150175-18150194 Msa0841660:five_prime_UTR 35.0%
AGAAATTACAGATCACTGGT+TGG - chr5_4:18150231-18150250 Msa0841660:five_prime_UTR 35.0%
GGTCCAATTATTCATGATAG+AGG - chr5_4:18148734-18148753 Msa0841660:three_prime_UTR 35.0%
! TAAACCAATAAGCTTTGTGC+AGG - chr5_4:18149033-18149052 Msa0841660:three_prime_UTR 35.0%
GTAGTTCAAGACAAAAACTC+AGG - chr5_4:18149062-18149081 Msa0841660:exon 35.0%
GTTGGACATGTGTTATAACT+AGG - chr5_4:18149123-18149142 Msa0841660:CDS 35.0%
TGTGTTATAACTAGGACTAC+TGG - chr5_4:18149131-18149150 Msa0841660:CDS 35.0%
! AATGAATTGGTTGGTTCAAC+TGG - chr5_4:18149315-18149334 Msa0841660:intron 35.0%
AATAGGACTCTGGATCAAAT+CGG + chr5_4:18149539-18149558 None:intergenic 35.0%
TCTGAAAAACAATAGGACTC+TGG + chr5_4:18149549-18149568 None:intergenic 35.0%
!! GATGGAGTTGTTCCATTTTT+AGG - chr5_4:18149782-18149801 Msa0841660:CDS 35.0%
!!! TGTTCCATTTTTAGGACTGA+AGG - chr5_4:18149790-18149809 Msa0841660:CDS 35.0%
CTTAGTGAATCACTTCTCTA+GGG + chr5_4:18149898-18149917 None:intergenic 35.0%
CTAGAGAAGTGATTCACTAA+GGG - chr5_4:18149897-18149916 Msa0841660:intron 35.0%
TGTGTGATCAGCTATGAATT+TGG - chr5_4:18149986-18150005 Msa0841660:intron 35.0%
! TATAAGAACAGTCTGAAGCA+TGG - chr5_4:18150175-18150194 Msa0841660:five_prime_UTR 35.0%
AGAAATTACAGATCACTGGT+TGG - chr5_4:18150231-18150250 Msa0841660:five_prime_UTR 35.0%
CTTTCCTGCACAAAGCTTAT+TGG + chr5_4:18149040-18149059 None:intergenic 40.0%
! ATAAGCTTTGTGCAGGAAAG+TGG - chr5_4:18149040-18149059 Msa0841660:three_prime_UTR 40.0%
ACAAAAACTTTGCCAGCATC+AGG + chr5_4:18149158-18149177 None:intergenic 40.0%
TGAGCTGACAACAGTTGAAA+GGG + chr5_4:18149425-18149444 None:intergenic 40.0%
TTGAGCTGACAACAGTTGAA+AGG + chr5_4:18149426-18149445 None:intergenic 40.0%
TCACTGCTACTCTCAATTTC+AGG + chr5_4:18149727-18149746 None:intergenic 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - chr5_4:18149730-18149749 Msa0841660:intron 40.0%
AGTGAAACATTCTGCAAGTG+TGG - chr5_4:18149754-18149773 Msa0841660:CDS 40.0%
AGGACCTTCAGTCCTAAAAA+TGG + chr5_4:18149797-18149816 None:intergenic 40.0%
! TCACTTCTCTAGGGACAATT+TGG + chr5_4:18149889-18149908 None:intergenic 40.0%
CCTTAGTGAATCACTTCTCT+AGG + chr5_4:18149899-18149918 None:intergenic 40.0%
CCTAGAGAAGTGATTCACTA+AGG - chr5_4:18149896-18149915 Msa0841660:intron 40.0%
CTTTCCTGCACAAAGCTTAT+TGG + chr5_4:18149040-18149059 None:intergenic 40.0%
! ATAAGCTTTGTGCAGGAAAG+TGG - chr5_4:18149040-18149059 Msa0841660:three_prime_UTR 40.0%
ACAAAAACTTTGCCAGCATC+AGG + chr5_4:18149158-18149177 None:intergenic 40.0%
TGAGCTGACAACAGTTGAAA+GGG + chr5_4:18149425-18149444 None:intergenic 40.0%
TTGAGCTGACAACAGTTGAA+AGG + chr5_4:18149426-18149445 None:intergenic 40.0%
TCACTGCTACTCTCAATTTC+AGG + chr5_4:18149727-18149746 None:intergenic 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - chr5_4:18149730-18149749 Msa0841660:intron 40.0%
AGTGAAACATTCTGCAAGTG+TGG - chr5_4:18149754-18149773 Msa0841660:CDS 40.0%
AGGACCTTCAGTCCTAAAAA+TGG + chr5_4:18149797-18149816 None:intergenic 40.0%
! TCACTTCTCTAGGGACAATT+TGG + chr5_4:18149889-18149908 None:intergenic 40.0%
CCTTAGTGAATCACTTCTCT+AGG + chr5_4:18149899-18149918 None:intergenic 40.0%
CCTAGAGAAGTGATTCACTA+AGG - chr5_4:18149896-18149915 Msa0841660:intron 40.0%
! AGGGAAGTTCCGACTTTTCA+AGG - chr5_4:18148754-18148773 Msa0841660:three_prime_UTR 45.0%
GAGGTAGAGCCTTGAAAAGT+CGG + chr5_4:18148766-18148785 None:intergenic 45.0%
TTGCTCTTGAGTGCAGCTTT+GGG + chr5_4:18148877-18148896 None:intergenic 45.0%
AAAGCTGCACTCAAGAGCAA+GGG - chr5_4:18148877-18148896 Msa0841660:three_prime_UTR 45.0%
TGGAACAAGAGTAGTATGGG+TGG + chr5_4:18148988-18149007 None:intergenic 45.0%
TCAAGACAAAAACTCAGGCC+TGG - chr5_4:18149067-18149086 Msa0841660:exon 45.0%
CAAGACAAAAACTCAGGCCT+GGG - chr5_4:18149068-18149087 Msa0841660:exon 45.0%
AAAAACTCAGGCCTGGGTTT+GGG - chr5_4:18149074-18149093 Msa0841660:CDS 45.0%
GTGTTGTGCAAGACTGGTAA+AGG - chr5_4:18149101-18149120 Msa0841660:CDS 45.0%
TGTGCAAGACTGGTAAAGGT+TGG - chr5_4:18149105-18149124 Msa0841660:CDS 45.0%
AACTCTTGTGCATGCAGAGA+TGG - chr5_4:18149688-18149707 Msa0841660:intron 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - chr5_4:18149760-18149779 Msa0841660:CDS 45.0%
! AGGGAAGTTCCGACTTTTCA+AGG - chr5_4:18148754-18148773 Msa0841660:three_prime_UTR 45.0%
GAGGTAGAGCCTTGAAAAGT+CGG + chr5_4:18148766-18148785 None:intergenic 45.0%
TTGCTCTTGAGTGCAGCTTT+GGG + chr5_4:18148877-18148896 None:intergenic 45.0%
AAAGCTGCACTCAAGAGCAA+GGG - chr5_4:18148877-18148896 Msa0841660:three_prime_UTR 45.0%
TGGAACAAGAGTAGTATGGG+TGG + chr5_4:18148988-18149007 None:intergenic 45.0%
TCAAGACAAAAACTCAGGCC+TGG - chr5_4:18149067-18149086 Msa0841660:exon 45.0%
CAAGACAAAAACTCAGGCCT+GGG - chr5_4:18149068-18149087 Msa0841660:exon 45.0%
AAAAACTCAGGCCTGGGTTT+GGG - chr5_4:18149074-18149093 Msa0841660:CDS 45.0%
GTGTTGTGCAAGACTGGTAA+AGG - chr5_4:18149101-18149120 Msa0841660:CDS 45.0%
TGTGCAAGACTGGTAAAGGT+TGG - chr5_4:18149105-18149124 Msa0841660:CDS 45.0%
AACTCTTGTGCATGCAGAGA+TGG - chr5_4:18149688-18149707 Msa0841660:intron 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - chr5_4:18149760-18149779 Msa0841660:CDS 45.0%
!! AAAAAAAAAAAAAAACTAAT+AGG + chr5_4:18150142-18150161 None:intergenic 5.0%
!! AAAAAAAAAAAAAAACTAAT+AGG + chr5_4:18150142-18150161 None:intergenic 5.0%
CTTGCTCTTGAGTGCAGCTT+TGG + chr5_4:18148878-18148897 None:intergenic 50.0%
CAAAGCTGCACTCAAGAGCA+AGG - chr5_4:18148876-18148895 Msa0841660:three_prime_UTR 50.0%
CAAAAACTCAGGCCTGGGTT+TGG - chr5_4:18149073-18149092 Msa0841660:CDS 50.0%
GTGTGTGACATACAGTGTGC+AGG + chr5_4:18149201-18149220 None:intergenic 50.0%
TCTTGTGCATGCAGAGATGG+TGG - chr5_4:18149691-18149710 Msa0841660:intron 50.0%
TCTGCAAGTGTGGTGAAGGA+TGG - chr5_4:18149764-18149783 Msa0841660:CDS 50.0%
CTTGCTCTTGAGTGCAGCTT+TGG + chr5_4:18148878-18148897 None:intergenic 50.0%
CAAAGCTGCACTCAAGAGCA+AGG - chr5_4:18148876-18148895 Msa0841660:three_prime_UTR 50.0%
CAAAAACTCAGGCCTGGGTT+TGG - chr5_4:18149073-18149092 Msa0841660:CDS 50.0%
GTGTGTGACATACAGTGTGC+AGG + chr5_4:18149201-18149220 None:intergenic 50.0%
TCTTGTGCATGCAGAGATGG+TGG - chr5_4:18149691-18149710 Msa0841660:intron 50.0%
TCTGCAAGTGTGGTGAAGGA+TGG - chr5_4:18149764-18149783 Msa0841660:CDS 50.0%
ACAACACACTGCCCAAACCC+AGG + chr5_4:18149088-18149107 None:intergenic 55.0%
GGCAGTGTGTTGTGCAAGAC+TGG - chr5_4:18149095-18149114 Msa0841660:CDS 55.0%
AGGACTACTGGACCTGATGC+TGG - chr5_4:18149143-18149162 Msa0841660:CDS 55.0%
GAAGGTCCTGATGCAGACTC+AGG - chr5_4:18149808-18149827 Msa0841660:CDS 55.0%
TCCTGATGCAGACTCAGGCA+AGG - chr5_4:18149813-18149832 Msa0841660:CDS 55.0%
CCTGATGCAGACTCAGGCAA+GGG - chr5_4:18149814-18149833 Msa0841660:CDS 55.0%
ACAACACACTGCCCAAACCC+AGG + chr5_4:18149088-18149107 None:intergenic 55.0%
GGCAGTGTGTTGTGCAAGAC+TGG - chr5_4:18149095-18149114 Msa0841660:CDS 55.0%
AGGACTACTGGACCTGATGC+TGG - chr5_4:18149143-18149162 Msa0841660:CDS 55.0%
GAAGGTCCTGATGCAGACTC+AGG - chr5_4:18149808-18149827 Msa0841660:CDS 55.0%
TCCTGATGCAGACTCAGGCA+AGG - chr5_4:18149813-18149832 Msa0841660:CDS 55.0%
CCTGATGCAGACTCAGGCAA+GGG - chr5_4:18149814-18149833 Msa0841660:CDS 55.0%
CCCTTGCCTGAGTCTGCATC+AGG + chr5_4:18149817-18149836 None:intergenic 60.0%
CCCTTGCCTGAGTCTGCATC+AGG + chr5_4:18149817-18149836 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 18148668 18150285 18148668 ID=Msa0841660;Name=Msa0841660
chr5_4 mRNA 18148668 18150285 18148668 ID=Msa0841660-mRNA-1;Parent=Msa0841660;Name=Msa0841660-mRNA-1;_AED=0.15;_eAED=0.15;_QI=142|1|1|1|1|1|3|403|139
chr5_4 exon 18148668 18149248 18148668 ID=Msa0841660-mRNA-1:exon:7635;Parent=Msa0841660-mRNA-1
chr5_4 exon 18149746 18149898 18149746 ID=Msa0841660-mRNA-1:exon:7634;Parent=Msa0841660-mRNA-1
chr5_4 exon 18150055 18150285 18150055 ID=Msa0841660-mRNA-1:exon:7633;Parent=Msa0841660-mRNA-1
chr5_4 five_prime_UTR 18150144 18150285 18150144 ID=Msa0841660-mRNA-1:five_prime_utr;Parent=Msa0841660-mRNA-1
chr5_4 CDS 18150055 18150143 18150055 ID=Msa0841660-mRNA-1:cds;Parent=Msa0841660-mRNA-1
chr5_4 CDS 18149746 18149898 18149746 ID=Msa0841660-mRNA-1:cds;Parent=Msa0841660-mRNA-1
chr5_4 CDS 18149071 18149248 18149071 ID=Msa0841660-mRNA-1:cds;Parent=Msa0841660-mRNA-1
chr5_4 three_prime_UTR 18148668 18149070 18148668 ID=Msa0841660-mRNA-1:three_prime_utr;Parent=Msa0841660-mRNA-1
Gene Sequence

>Msa0841660

ATGACAAAATTGATAAGCAAAAGAAATCAATCATACTACTCATATCATTGTGAGAATATGGAGTCCCAAAGCTGCACTCAAGAGCAAGGGAAAGTGGTAGTTCAAGACAAAAACTCAGGCCTGGGTTTGGGCAGTGTGTTGTGCAAGACTGGTAAAGGTTGGACATGTGTTATAACTAGGACTACTGGACCTGATGCTGGCAAAGTTTTTGTTAAATGCGGTGAAAATTGCACCTGCACACTAGATGGTGGAGCTGTGACACCTGAAATTGAGAGTAGCAGTGACAATGGCAGTGAAACATTCTGCAAGTGTGGTGAAGGATGGAGTTGTTCCATTTTTAGGACTGAAGGTCCTGATGCAGACTCAGGCAAGGGATTTGCTGATTGCACTCAACAGTATAACTGTGCTTGTAAATGTTGA
Protein sequence

>Msa0841660

MTKLISKRNQSYYSYHCENMESQSCTQEQGKVVVQDKNSGLGLGSVLCKTGKGWTCVITRTTGPDAGKVFVKCGENCTCTLDGGAVTPEIESSSDNGSETFCKCGEGWSCSIFRTEGPDADSGKGFADCTQQYNCACKC*