AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0720480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0720480 MtrunA17_Chr5g0411231 95.833 120 5 0 1 120 1 120 8.05e-81 233
Msa0720480 MtrunA17_Chr5g0411241 85.000 120 18 0 1 120 1 120 5.92e-70 205
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTAATTTGTACTAAATT+TGG 0.168371 5_1:-16519746 Msa0720480:three_prime_UTR
TGTTTGATCGGCTATGAATT+TGG 0.230112 5_1:-16519707 Msa0720480:three_prime_UTR
TCACTTCTCTAGGGACAATT+TGG 0.245955 5_1:+16519808 None:intergenic
CTTGCTCTTGAGTGCAGCTT+TGG 0.250449 5_1:+16520812 None:intergenic
TTCCCTCTATCATGAATAAT+TGG 0.255625 5_1:+16520946 None:intergenic
TCACTGCTACTCTCAATTTC+AGG 0.257468 5_1:+16519970 None:intergenic
CTCATATCATTGTGAGAATA+TGG 0.263715 5_1:-16520839 Msa0720480:exon
TTTAAGAATTAAGAAATTAC+TGG 0.290187 5_1:-16519471 Msa0720480:three_prime_UTR
TTGCTCTTGAGTGCAGCTTT+GGG 0.361214 5_1:+16520813 None:intergenic
CCCTTGCCTGAGTCTGCATC+AGG 0.380799 5_1:+16519880 None:intergenic
GTACTAAATTTGGTTTATCA+TGG 0.395419 5_1:-16519736 Msa0720480:three_prime_UTR
GTTTGTAATTAGTATGTGTT+TGG 0.396742 5_1:-16519671 Msa0720480:three_prime_UTR
GGTCCAATTATTCATGATAG+AGG 0.415239 5_1:-16520949 Msa0720480:five_prime_UTR
TAGAGAAGTGATTCACTAAA+GGG 0.449627 5_1:-16519796 Msa0720480:three_prime_UTR
CTTTAGTGAATCACTTCTCT+AGG 0.463477 5_1:+16519798 None:intergenic
GTAATGCAATAGTATGTGTT+TGG 0.464999 5_1:-16519633 Msa0720480:three_prime_UTR
TACTAAATTTGGTTTATCAT+GGG 0.470104 5_1:-16519735 Msa0720480:three_prime_UTR
GGCAATGTGTTGTGCAAGAC+TGG 0.472681 5_1:-16520606 Msa0720480:CDS
TTCAAGCTAATAAAATATCA+AGG 0.479076 5_1:-16519770 Msa0720480:three_prime_UTR
AGGTTCAATTATTGAGAAAG+AGG 0.485478 5_1:-16520992 Msa0720480:five_prime_UTR
TGTGTTATAACTAGGACTAC+TGG 0.487878 5_1:-16520570 Msa0720480:CDS
CTAGAGAAGTGATTCACTAA+AGG 0.500118 5_1:-16519797 Msa0720480:three_prime_UTR
GAGGTAGAGCCTTGAAAAGT+CGG 0.524080 5_1:+16520920 None:intergenic
TCATGGGTATTATGTTTGAT+CGG 0.526500 5_1:-16519719 Msa0720480:three_prime_UTR
AATGTATCAACTAGTGAATG+AGG 0.527069 5_1:+16520901 None:intergenic
AGGACTACTGGACCTGATGC+TGG 0.536873 5_1:-16520558 Msa0720480:CDS
GAAGGCCCTGATGCAGACTC+AGG 0.558417 5_1:-16519886 Msa0720480:CDS
TTTAGTGAATCACTTCTCTA+GGG 0.558643 5_1:+16519799 None:intergenic
CAAAGCTGCACTCAAGAGCA+AGG 0.561178 5_1:-16520811 Msa0720480:CDS
CCCTGATGCAGACTCAGGCA+AGG 0.569002 5_1:-16519881 Msa0720480:CDS
CCTTGCCTGAGTCTGCATCA+GGG 0.572670 5_1:+16519881 None:intergenic
GTCCAATTATTCATGATAGA+GGG 0.575626 5_1:-16520948 Msa0720480:five_prime_UTR
GTTGGACATGTGTTATAACT+AGG 0.581816 5_1:-16520578 Msa0720480:CDS
TCTGCAAGTGTGGTGAAGGA+TGG 0.591787 5_1:-16519930 Msa0720480:CDS
ACATTCTGCAAGTGTGGTGA+AGG 0.593456 5_1:-16519934 Msa0720480:CDS
TACTAATTACAAACATCATG+TGG 0.601550 5_1:+16519680 None:intergenic
GTGTGTGACATACAGTGTGC+AGG 0.603372 5_1:+16520503 None:intergenic
GCTGTTTATGACTTTAAACA+TGG 0.619952 5_1:-16520970 Msa0720480:five_prime_UTR
ATTGAGAGTAGCAGTGACAA+TGG 0.620823 5_1:-16519964 Msa0720480:CDS
TCTTGTGCATGCAGTGATGG+TGG 0.622946 5_1:-16520003 Msa0720480:intron
CCTGATGCAGACTCAGGCAA+GGG 0.625232 5_1:-16519880 Msa0720480:CDS
GTGTTGTGCAAGACTGGTAA+AGG 0.626364 5_1:-16520600 Msa0720480:CDS
TATAAGAACAGTCTGAAGCA+TGG 0.629013 5_1:-16519516 Msa0720480:three_prime_UTR
AAAGCTGCACTCAAGAGCAA+GGG 0.647133 5_1:-16520810 Msa0720480:intron
ACAACACATTGCCCAAACCC+AGG 0.649057 5_1:+16520616 None:intergenic
TGTGCAAGACTGGTAAAGGT+TGG 0.662647 5_1:-16520596 Msa0720480:CDS
AGTGAAACATTCTGCAAGTG+TGG 0.671634 5_1:-16519940 Msa0720480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGAAATATAATTCATTTT+TGG - chr5_1:16520385-16520404 Msa0720480:intron 10.0%
!!! TAATTAATTTGTACTAAATT+TGG - chr5_1:16520708-16520727 Msa0720480:intron 10.0%
!!! AAAGAAATATAATTCATTTT+TGG - chr5_1:16520385-16520404 Msa0720480:intron 10.0%
!!! TAATTAATTTGTACTAAATT+TGG - chr5_1:16520708-16520727 Msa0720480:intron 10.0%
!! AAAACTATATAGATGAAATA+AGG + chr5_1:16519697-16519716 None:intergenic 15.0%
!!! TTTCATTTTTTAGATTGAAT+TGG - chr5_1:16520144-16520163 Msa0720480:intron 15.0%
!!! TTCATTTTTTAGATTGAATT+GGG - chr5_1:16520145-16520164 Msa0720480:intron 15.0%
!! AAAAAAAACTAATACTAACA+AGG + chr5_1:16520906-16520925 None:intergenic 15.0%
!! TTTAAGAATTAAGAAATTAC+TGG - chr5_1:16520983-16521002 Msa0720480:five_prime_UTR 15.0%
!! AAAACTATATAGATGAAATA+AGG + chr5_1:16519697-16519716 None:intergenic 15.0%
!!! TTTCATTTTTTAGATTGAAT+TGG - chr5_1:16520144-16520163 Msa0720480:intron 15.0%
!!! TTCATTTTTTAGATTGAATT+GGG - chr5_1:16520145-16520164 Msa0720480:intron 15.0%
!! AAAAAAAACTAATACTAACA+AGG + chr5_1:16520906-16520925 None:intergenic 15.0%
!! TTTAAGAATTAAGAAATTAC+TGG - chr5_1:16520983-16521002 Msa0720480:five_prime_UTR 15.0%
!! GATTTATTTATGCATGAAAA+TGG + chr5_1:16519761-16519780 None:intergenic 20.0%
!! GTAAAATTAAAATAGGCAAA+AGG + chr5_1:16519981-16520000 None:intergenic 20.0%
!! GATGGTAGTAAAATTAAAAT+AGG + chr5_1:16519988-16520007 None:intergenic 20.0%
!!! TTTTTCAAAATCGAATGAAT+TGG - chr5_1:16520055-16520074 Msa0720480:intron 20.0%
!!! AGTTAATTCTGAAAAACAAT+AGG + chr5_1:16520302-16520321 None:intergenic 20.0%
!! ATTAACTCTTGAGATAAAAT+CGG - chr5_1:16520315-16520334 Msa0720480:intron 20.0%
!! TTCAAGCTAATAAAATATCA+AGG - chr5_1:16520684-16520703 Msa0720480:intron 20.0%
!!! TACTAAATTTGGTTTATCAT+GGG - chr5_1:16520719-16520738 Msa0720480:intron 20.0%
!! GATTTATTTATGCATGAAAA+TGG + chr5_1:16519761-16519780 None:intergenic 20.0%
!! GTAAAATTAAAATAGGCAAA+AGG + chr5_1:16519981-16520000 None:intergenic 20.0%
!! GATGGTAGTAAAATTAAAAT+AGG + chr5_1:16519988-16520007 None:intergenic 20.0%
!!! TTTTTCAAAATCGAATGAAT+TGG - chr5_1:16520055-16520074 Msa0720480:intron 20.0%
!!! AGTTAATTCTGAAAAACAAT+AGG + chr5_1:16520302-16520321 None:intergenic 20.0%
!! ATTAACTCTTGAGATAAAAT+CGG - chr5_1:16520315-16520334 Msa0720480:intron 20.0%
!! TTCAAGCTAATAAAATATCA+AGG - chr5_1:16520684-16520703 Msa0720480:intron 20.0%
!!! TACTAAATTTGGTTTATCAT+GGG - chr5_1:16520719-16520738 Msa0720480:intron 20.0%
! TTTGATCAAAATCATTTCGA+TGG + chr5_1:16520006-16520025 None:intergenic 25.0%
! AATGAAACTGCAAAGTAATA+AGG + chr5_1:16520131-16520150 None:intergenic 25.0%
! TGTTGTAACAATAGTTGAAT+CGG + chr5_1:16520253-16520272 None:intergenic 25.0%
! TAAATATAAGCCTAGATTTG+TGG + chr5_1:16520366-16520385 None:intergenic 25.0%
!! GTACTAAATTTGGTTTATCA+TGG - chr5_1:16520718-16520737 Msa0720480:intron 25.0%
! TACTAATTACAAACATCATG+TGG + chr5_1:16520777-16520796 None:intergenic 25.0%
! GTTTGTAATTAGTATGTGTT+TGG - chr5_1:16520783-16520802 Msa0720480:intron 25.0%
! TTTGATCAAAATCATTTCGA+TGG + chr5_1:16520006-16520025 None:intergenic 25.0%
! AATGAAACTGCAAAGTAATA+AGG + chr5_1:16520131-16520150 None:intergenic 25.0%
! TGTTGTAACAATAGTTGAAT+CGG + chr5_1:16520253-16520272 None:intergenic 25.0%
! TAAATATAAGCCTAGATTTG+TGG + chr5_1:16520366-16520385 None:intergenic 25.0%
!! GTACTAAATTTGGTTTATCA+TGG - chr5_1:16520718-16520737 Msa0720480:intron 25.0%
! TACTAATTACAAACATCATG+TGG + chr5_1:16520777-16520796 None:intergenic 25.0%
! GTTTGTAATTAGTATGTGTT+TGG - chr5_1:16520783-16520802 Msa0720480:intron 25.0%
AGGTTCAATTATTGAGAAAG+AGG - chr5_1:16519462-16519481 Msa0720480:three_prime_UTR 30.0%
GCTGTTTATGACTTTAAACA+TGG - chr5_1:16519484-16519503 Msa0720480:three_prime_UTR 30.0%
GTCCAATTATTCATGATAGA+GGG - chr5_1:16519506-16519525 Msa0720480:three_prime_UTR 30.0%
TTCCCTCTATCATGAATAAT+TGG + chr5_1:16519511-16519530 None:intergenic 30.0%
AATGTATCAACTAGTGAATG+AGG + chr5_1:16519556-16519575 None:intergenic 30.0%
CTCATATCATTGTGAGAATA+TGG - chr5_1:16519615-16519634 Msa0720480:three_prime_UTR 30.0%
! AAATGGAACATGAAGAGTAT+TGG + chr5_1:16519744-16519763 None:intergenic 30.0%
ATAGTTCAAGACAAAAACTC+AGG - chr5_1:16519815-16519834 Msa0720480:exon 30.0%
! GGCAAAGTTTTTGTTAAATG+CGG - chr5_1:16519917-16519936 Msa0720480:CDS 30.0%
TCAAAATCGAATGAATTGGT+TGG - chr5_1:16520059-16520078 Msa0720480:intron 30.0%
AATAAGGATCAAATCGATTG+AGG + chr5_1:16520115-16520134 None:intergenic 30.0%
AGATTTGTGGCATATTTGAA+TGG + chr5_1:16520353-16520372 None:intergenic 30.0%
TTCAAATATGCCACAAATCT+AGG - chr5_1:16520353-16520372 Msa0720480:intron 30.0%
ATCTAGCAGTGCAATATATA+TGG + chr5_1:16520421-16520440 None:intergenic 30.0%
TTTAGTGAATCACTTCTCTA+GGG + chr5_1:16520658-16520677 None:intergenic 30.0%
TAGAGAAGTGATTCACTAAA+GGG - chr5_1:16520658-16520677 Msa0720480:intron 30.0%
TCATGGGTATTATGTTTGAT+CGG - chr5_1:16520735-16520754 Msa0720480:intron 30.0%
GTAATGCAATAGTATGTGTT+TGG - chr5_1:16520821-16520840 Msa0720480:CDS 30.0%
!! GAAATTTTAAGAGACAACAG+AGG + chr5_1:16520873-16520892 None:intergenic 30.0%
AGGTTCAATTATTGAGAAAG+AGG - chr5_1:16519462-16519481 Msa0720480:three_prime_UTR 30.0%
GCTGTTTATGACTTTAAACA+TGG - chr5_1:16519484-16519503 Msa0720480:three_prime_UTR 30.0%
GTCCAATTATTCATGATAGA+GGG - chr5_1:16519506-16519525 Msa0720480:three_prime_UTR 30.0%
TTCCCTCTATCATGAATAAT+TGG + chr5_1:16519511-16519530 None:intergenic 30.0%
AATGTATCAACTAGTGAATG+AGG + chr5_1:16519556-16519575 None:intergenic 30.0%
CTCATATCATTGTGAGAATA+TGG - chr5_1:16519615-16519634 Msa0720480:three_prime_UTR 30.0%
! AAATGGAACATGAAGAGTAT+TGG + chr5_1:16519744-16519763 None:intergenic 30.0%
ATAGTTCAAGACAAAAACTC+AGG - chr5_1:16519815-16519834 Msa0720480:exon 30.0%
! GGCAAAGTTTTTGTTAAATG+CGG - chr5_1:16519917-16519936 Msa0720480:CDS 30.0%
TCAAAATCGAATGAATTGGT+TGG - chr5_1:16520059-16520078 Msa0720480:intron 30.0%
AATAAGGATCAAATCGATTG+AGG + chr5_1:16520115-16520134 None:intergenic 30.0%
AGATTTGTGGCATATTTGAA+TGG + chr5_1:16520353-16520372 None:intergenic 30.0%
TTCAAATATGCCACAAATCT+AGG - chr5_1:16520353-16520372 Msa0720480:intron 30.0%
ATCTAGCAGTGCAATATATA+TGG + chr5_1:16520421-16520440 None:intergenic 30.0%
TTTAGTGAATCACTTCTCTA+GGG + chr5_1:16520658-16520677 None:intergenic 30.0%
TAGAGAAGTGATTCACTAAA+GGG - chr5_1:16520658-16520677 Msa0720480:intron 30.0%
TCATGGGTATTATGTTTGAT+CGG - chr5_1:16520735-16520754 Msa0720480:intron 30.0%
GTAATGCAATAGTATGTGTT+TGG - chr5_1:16520821-16520840 Msa0720480:CDS 30.0%
!! GAAATTTTAAGAGACAACAG+AGG + chr5_1:16520873-16520892 None:intergenic 30.0%
!! GCAGCAGTATTTTTCAATCT+AGG - chr5_1:16519442-16519461 Msa0720480:three_prime_UTR 35.0%
GGTCCAATTATTCATGATAG+AGG - chr5_1:16519505-16519524 Msa0720480:three_prime_UTR 35.0%
! TAAACCAATAAGCTTTGTGC+AGG - chr5_1:16519786-16519805 Msa0720480:three_prime_UTR 35.0%
GTTGGACATGTGTTATAACT+AGG - chr5_1:16519876-16519895 Msa0720480:CDS 35.0%
TGTGTTATAACTAGGACTAC+TGG - chr5_1:16519884-16519903 Msa0720480:CDS 35.0%
! AATGAATTGGTTGGTTCAAC+TGG - chr5_1:16520068-16520087 Msa0720480:intron 35.0%
!!! GCGCAACTAATTGTCATTTT+AGG - chr5_1:16520188-16520207 Msa0720480:intron 35.0%
! AATAGGACTCTGGTTCAAAT+CGG + chr5_1:16520285-16520304 None:intergenic 35.0%
TCTGAAAAACAATAGGACTC+TGG + chr5_1:16520295-16520314 None:intergenic 35.0%
!! GATGGAGTTGTTCCATTTTT+AGG - chr5_1:16520542-16520561 Msa0720480:CDS 35.0%
!!! TGTTCCATTTTTAGGACTGA+AGG - chr5_1:16520550-16520569 Msa0720480:CDS 35.0%
CTTTAGTGAATCACTTCTCT+AGG + chr5_1:16520659-16520678 None:intergenic 35.0%
CTAGAGAAGTGATTCACTAA+AGG - chr5_1:16520657-16520676 Msa0720480:intron 35.0%
TGTTTGATCGGCTATGAATT+TGG - chr5_1:16520747-16520766 Msa0720480:intron 35.0%
! TATAAGAACAGTCTGAAGCA+TGG - chr5_1:16520938-16520957 Msa0720480:five_prime_UTR 35.0%
!! GCAGCAGTATTTTTCAATCT+AGG - chr5_1:16519442-16519461 Msa0720480:three_prime_UTR 35.0%
GGTCCAATTATTCATGATAG+AGG - chr5_1:16519505-16519524 Msa0720480:three_prime_UTR 35.0%
! TAAACCAATAAGCTTTGTGC+AGG - chr5_1:16519786-16519805 Msa0720480:three_prime_UTR 35.0%
GTTGGACATGTGTTATAACT+AGG - chr5_1:16519876-16519895 Msa0720480:CDS 35.0%
TGTGTTATAACTAGGACTAC+TGG - chr5_1:16519884-16519903 Msa0720480:CDS 35.0%
! AATGAATTGGTTGGTTCAAC+TGG - chr5_1:16520068-16520087 Msa0720480:intron 35.0%
!!! GCGCAACTAATTGTCATTTT+AGG - chr5_1:16520188-16520207 Msa0720480:intron 35.0%
! AATAGGACTCTGGTTCAAAT+CGG + chr5_1:16520285-16520304 None:intergenic 35.0%
TCTGAAAAACAATAGGACTC+TGG + chr5_1:16520295-16520314 None:intergenic 35.0%
!! GATGGAGTTGTTCCATTTTT+AGG - chr5_1:16520542-16520561 Msa0720480:CDS 35.0%
!!! TGTTCCATTTTTAGGACTGA+AGG - chr5_1:16520550-16520569 Msa0720480:CDS 35.0%
CTTTAGTGAATCACTTCTCT+AGG + chr5_1:16520659-16520678 None:intergenic 35.0%
CTAGAGAAGTGATTCACTAA+AGG - chr5_1:16520657-16520676 Msa0720480:intron 35.0%
TGTTTGATCGGCTATGAATT+TGG - chr5_1:16520747-16520766 Msa0720480:intron 35.0%
! TATAAGAACAGTCTGAAGCA+TGG - chr5_1:16520938-16520957 Msa0720480:five_prime_UTR 35.0%
CTTTCCTGCACAAAGCTTAT+TGG + chr5_1:16519793-16519812 None:intergenic 40.0%
ACAAAAACTTTGCCAGCATC+AGG + chr5_1:16519911-16519930 None:intergenic 40.0%
TCACTGCTACTCTCAATTTC+AGG + chr5_1:16520487-16520506 None:intergenic 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - chr5_1:16520490-16520509 Msa0720480:intron 40.0%
AGTGAAACATTCTGCAAGTG+TGG - chr5_1:16520514-16520533 Msa0720480:intron 40.0%
! TCACTTCTCTAGGGACAATT+TGG + chr5_1:16520649-16520668 None:intergenic 40.0%
CTTTCCTGCACAAAGCTTAT+TGG + chr5_1:16519793-16519812 None:intergenic 40.0%
ACAAAAACTTTGCCAGCATC+AGG + chr5_1:16519911-16519930 None:intergenic 40.0%
TCACTGCTACTCTCAATTTC+AGG + chr5_1:16520487-16520506 None:intergenic 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - chr5_1:16520490-16520509 Msa0720480:intron 40.0%
AGTGAAACATTCTGCAAGTG+TGG - chr5_1:16520514-16520533 Msa0720480:intron 40.0%
! TCACTTCTCTAGGGACAATT+TGG + chr5_1:16520649-16520668 None:intergenic 40.0%
! AGGGAAGTTCCGACTTTTCA+AGG - chr5_1:16519525-16519544 Msa0720480:three_prime_UTR 45.0%
GAGGTAGAGCCTTGAAAAGT+CGG + chr5_1:16519537-16519556 None:intergenic 45.0%
TTGCTCTTGAGTGCAGCTTT+GGG + chr5_1:16519644-16519663 None:intergenic 45.0%
AAAGCTGCACTCAAGAGCAA+GGG - chr5_1:16519644-16519663 Msa0720480:three_prime_UTR 45.0%
TCAAGACAAAAACTCAGGCC+TGG - chr5_1:16519820-16519839 Msa0720480:exon 45.0%
CAAGACAAAAACTCAGGCCT+GGG - chr5_1:16519821-16519840 Msa0720480:exon 45.0%
AAAAACTCAGGCCTGGGTTT+GGG - chr5_1:16519827-16519846 Msa0720480:exon 45.0%
GTGTTGTGCAAGACTGGTAA+AGG - chr5_1:16519854-16519873 Msa0720480:CDS 45.0%
TGTGCAAGACTGGTAAAGGT+TGG - chr5_1:16519858-16519877 Msa0720480:CDS 45.0%
AACTCTTGTGCATGCAGTGA+TGG - chr5_1:16520448-16520467 Msa0720480:intron 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - chr5_1:16520520-16520539 Msa0720480:CDS 45.0%
AGGGCCTTCAGTCCTAAAAA+TGG + chr5_1:16520557-16520576 None:intergenic 45.0%
! AGGGAAGTTCCGACTTTTCA+AGG - chr5_1:16519525-16519544 Msa0720480:three_prime_UTR 45.0%
GAGGTAGAGCCTTGAAAAGT+CGG + chr5_1:16519537-16519556 None:intergenic 45.0%
TTGCTCTTGAGTGCAGCTTT+GGG + chr5_1:16519644-16519663 None:intergenic 45.0%
AAAGCTGCACTCAAGAGCAA+GGG - chr5_1:16519644-16519663 Msa0720480:three_prime_UTR 45.0%
TCAAGACAAAAACTCAGGCC+TGG - chr5_1:16519820-16519839 Msa0720480:exon 45.0%
CAAGACAAAAACTCAGGCCT+GGG - chr5_1:16519821-16519840 Msa0720480:exon 45.0%
AAAAACTCAGGCCTGGGTTT+GGG - chr5_1:16519827-16519846 Msa0720480:exon 45.0%
GTGTTGTGCAAGACTGGTAA+AGG - chr5_1:16519854-16519873 Msa0720480:CDS 45.0%
TGTGCAAGACTGGTAAAGGT+TGG - chr5_1:16519858-16519877 Msa0720480:CDS 45.0%
AACTCTTGTGCATGCAGTGA+TGG - chr5_1:16520448-16520467 Msa0720480:intron 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - chr5_1:16520520-16520539 Msa0720480:CDS 45.0%
AGGGCCTTCAGTCCTAAAAA+TGG + chr5_1:16520557-16520576 None:intergenic 45.0%
CTTGCTCTTGAGTGCAGCTT+TGG + chr5_1:16519645-16519664 None:intergenic 50.0%
CAAAGCTGCACTCAAGAGCA+AGG - chr5_1:16519643-16519662 Msa0720480:three_prime_UTR 50.0%
CAAAAACTCAGGCCTGGGTT+TGG - chr5_1:16519826-16519845 Msa0720480:exon 50.0%
ACAACACATTGCCCAAACCC+AGG + chr5_1:16519841-16519860 None:intergenic 50.0%
GGCAATGTGTTGTGCAAGAC+TGG - chr5_1:16519848-16519867 Msa0720480:CDS 50.0%
GTGTGTGACATACAGTGTGC+AGG + chr5_1:16519954-16519973 None:intergenic 50.0%
TCTTGTGCATGCAGTGATGG+TGG - chr5_1:16520451-16520470 Msa0720480:intron 50.0%
TCTGCAAGTGTGGTGAAGGA+TGG - chr5_1:16520524-16520543 Msa0720480:CDS 50.0%
CTTGCTCTTGAGTGCAGCTT+TGG + chr5_1:16519645-16519664 None:intergenic 50.0%
CAAAGCTGCACTCAAGAGCA+AGG - chr5_1:16519643-16519662 Msa0720480:three_prime_UTR 50.0%
CAAAAACTCAGGCCTGGGTT+TGG - chr5_1:16519826-16519845 Msa0720480:exon 50.0%
ACAACACATTGCCCAAACCC+AGG + chr5_1:16519841-16519860 None:intergenic 50.0%
GGCAATGTGTTGTGCAAGAC+TGG - chr5_1:16519848-16519867 Msa0720480:CDS 50.0%
GTGTGTGACATACAGTGTGC+AGG + chr5_1:16519954-16519973 None:intergenic 50.0%
TCTTGTGCATGCAGTGATGG+TGG - chr5_1:16520451-16520470 Msa0720480:intron 50.0%
TCTGCAAGTGTGGTGAAGGA+TGG - chr5_1:16520524-16520543 Msa0720480:CDS 50.0%
AGGACTACTGGACCTGATGC+TGG - chr5_1:16519896-16519915 Msa0720480:CDS 55.0%
CCTTGCCTGAGTCTGCATCA+GGG + chr5_1:16520576-16520595 None:intergenic 55.0%
CCTGATGCAGACTCAGGCAA+GGG - chr5_1:16520574-16520593 Msa0720480:CDS 55.0%
AGGACTACTGGACCTGATGC+TGG - chr5_1:16519896-16519915 Msa0720480:CDS 55.0%
CCTTGCCTGAGTCTGCATCA+GGG + chr5_1:16520576-16520595 None:intergenic 55.0%
CCTGATGCAGACTCAGGCAA+GGG - chr5_1:16520574-16520593 Msa0720480:CDS 55.0%
GAAGGCCCTGATGCAGACTC+AGG - chr5_1:16520568-16520587 Msa0720480:CDS 60.0%
CCCTGATGCAGACTCAGGCA+AGG - chr5_1:16520573-16520592 Msa0720480:CDS 60.0%
CCCTTGCCTGAGTCTGCATC+AGG + chr5_1:16520577-16520596 None:intergenic 60.0%
GAAGGCCCTGATGCAGACTC+AGG - chr5_1:16520568-16520587 Msa0720480:CDS 60.0%
CCCTGATGCAGACTCAGGCA+AGG - chr5_1:16520573-16520592 Msa0720480:CDS 60.0%
CCCTTGCCTGAGTCTGCATC+AGG + chr5_1:16520577-16520596 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 16519439 16521037 16519439 ID=Msa0720480;Name=Msa0720480
chr5_1 mRNA 16519439 16521037 16519439 ID=Msa0720480-mRNA-1;Parent=Msa0720480;Name=Msa0720480-mRNA-1;_AED=0.14;_eAED=0.14;_QI=195|1|1|1|1|1|3|395|120
chr5_1 exon 16519439 16520011 16519439 ID=Msa0720480-mRNA-1:exon:6288;Parent=Msa0720480-mRNA-1
chr5_1 exon 16520516 16520668 16520516 ID=Msa0720480-mRNA-1:exon:6287;Parent=Msa0720480-mRNA-1
chr5_1 exon 16520811 16521037 16520811 ID=Msa0720480-mRNA-1:exon:6286;Parent=Msa0720480-mRNA-1
chr5_1 five_prime_UTR 16520843 16521037 16520843 ID=Msa0720480-mRNA-1:five_prime_utr;Parent=Msa0720480-mRNA-1
chr5_1 CDS 16520811 16520842 16520811 ID=Msa0720480-mRNA-1:cds;Parent=Msa0720480-mRNA-1
chr5_1 CDS 16520516 16520668 16520516 ID=Msa0720480-mRNA-1:cds;Parent=Msa0720480-mRNA-1
chr5_1 CDS 16519834 16520011 16519834 ID=Msa0720480-mRNA-1:cds;Parent=Msa0720480-mRNA-1
chr5_1 three_prime_UTR 16519439 16519833 16519439 ID=Msa0720480-mRNA-1:three_prime_utr;Parent=Msa0720480-mRNA-1
Gene Sequence

>Msa0720480

ATGGAGTCCCAAAGCTGCACTCAAGAGCAAGGGAAAGTGATAGTTCAAGACAAAAACTCAGGCCTGGGTTTGGGCAATGTGTTGTGCAAGACTGGTAAAGGTTGGACATGTGTTATAACTAGGACTACTGGACCTGATGCTGGCAAAGTTTTTGTTAAATGCGGTGAAAATTGCACCTGCACACTTGATGGTGGAGCTGTGACACCTGAAATTGAGAGTAGCAGTGACAATGGCAGTGAAACATTCTGCAAGTGTGGTGAAGGATGGAGTTGTTCCATTTTTAGGACTGAAGGCCCTGATGCAGACTCAGGCAAGGGATTTGCTGATTGCACTCAAGAGTATAACTGTGCTTGTAAATGTTGA
Protein sequence

>Msa0720480

MESQSCTQEQGKVIVQDKNSGLGLGNVLCKTGKGWTCVITRTTGPDAGKVFVKCGENCTCTLDGGAVTPEIESSSDNGSETFCKCGEGWSCSIFRTEGPDADSGKGFADCTQEYNCACKC*