AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0842840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0842840 MtrunA17_Chr1g0210231 75.000 52 10 2 1 51 225 274 9.53e-17 72.8
Msa0842840 MtrunA17_Chr8g0360481 65.385 52 15 2 1 51 207 256 5.87e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0842840 AT3G10420.2 71.154 52 12 2 1 51 236 285 1.18e-14 67.4
Msa0842840 AT3G10420.1 71.154 52 12 2 1 51 236 285 1.79e-14 66.6

Find 85 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCCTCCACTAACCTTAAT+TGG 0.118688 5_4:+20236246 Msa0842840:five_prime_UTR
CGGGGTTTGATTAGAGATTT+TGG 0.120378 5_4:-20236276 None:intergenic
AGGGGCGGTGGCGGCGGTTG+GGG 0.165599 5_4:-20236361 None:intergenic
ATGATTCTGTTCGGTTATTT+TGG 0.184356 5_4:-20236421 None:intergenic
CTGGTCCCTCATTTGGCTTT+GGG 0.251206 5_4:+20239890 Msa0842840:CDS
TCTGGTCCCTCATTTGGCTT+TGG 0.257586 5_4:+20239889 Msa0842840:CDS
GAGGGGCGGTGGCGGCGGTT+GGG 0.269031 5_4:-20236362 None:intergenic
GGTTCCTCTGGTCCCTCATT+TGG 0.282892 5_4:+20239883 Msa0842840:CDS
TTCAATGGGTTTAGTAATAT+CGG 0.290969 5_4:+20236691 Msa0842840:five_prime_UTR
TGGTATAAGGAAGCCGATTT+CGG 0.298681 5_4:-20236401 None:intergenic
ACAAAGATGATGCAAGAAAT+TGG 0.300447 5_4:+20239927 Msa0842840:three_prime_UTR
GTGGATACATCCAATGAAAT+TGG 0.303806 5_4:+20238006 Msa0842840:CDS
GGTGGAGATGTTCTTCTTGC+AGG 0.311714 5_4:+20238030 Msa0842840:CDS
TTCATTGCTGCTGGTTCCTC+TGG 0.329963 5_4:+20239871 Msa0842840:CDS
AGAGGGGCGGTGGCGGCGGT+TGG 0.333783 5_4:-20236363 None:intergenic
TCATCTTTGTCCTATTCTAT+AGG 0.336405 5_4:-20239914 None:intergenic
GGGGCGGTGGCGGCGGTTGG+GGG 0.352187 5_4:-20236360 None:intergenic
GCTGAGAAGAGGGGCGGTGG+CGG 0.366000 5_4:-20236370 None:intergenic
ATCTCCACCTCCAATTTCAT+TGG 0.369281 5_4:-20238016 None:intergenic
CAATGCTGCATATTCACATT+TGG 0.374960 5_4:-20238087 None:intergenic
TGTTGGCTCTTCATTGCTGC+TGG 0.375727 5_4:+20239862 Msa0842840:CDS
GTGGCTGAGAAGAGGGGCGG+TGG 0.376268 5_4:-20236373 None:intergenic
GAGAAGAGGGGCGGTGGCGG+CGG 0.394870 5_4:-20236367 None:intergenic
GGGCTTAAATACTACTTGTT+GGG 0.410633 5_4:+20240113 Msa0842840:three_prime_UTR
TGGGCTTAAATACTACTTGT+TGG 0.414964 5_4:+20240112 Msa0842840:three_prime_UTR
CTTGGCCCAATTAAGGTTAG+TGG 0.424230 5_4:-20236252 None:intergenic
GGCGGTGGTGGCTGAGAAGA+GGG 0.424722 5_4:-20236380 None:intergenic
GTGTGAAGGATTCAGCAATA+TGG 0.428666 5_4:+20240072 Msa0842840:three_prime_UTR
AAAGAGGATGCAAGTATTGT+TGG 0.439321 5_4:+20239845 Msa0842840:CDS
GTTGCTGTTAGATCTTTCAA+TGG 0.442599 5_4:+20236676 Msa0842840:intron
TCAGCCACCACCGCCGAAAT+CGG 0.444366 5_4:+20236388 Msa0842840:five_prime_UTR
CATGAAGCACGTAGCGATAG+TGG 0.449986 5_4:+20237987 Msa0842840:CDS
ATGACAAATGAGTGGGAGAG+AGG 0.451713 5_4:-20236305 None:intergenic
TTGCTGTTAGATCTTTCAAT+GGG 0.458390 5_4:+20236677 Msa0842840:intron
ACCCTCCACTAACCTTAATT+GGG 0.470096 5_4:+20236247 Msa0842840:five_prime_UTR
CAAATGTGAATATGCAGCAT+TGG 0.470780 5_4:+20238088 Msa0842840:CDS
GGCTTTGGGACCTATAGAAT+AGG 0.480062 5_4:+20239904 Msa0842840:CDS
GAAGCCGATTTCGGCGGTGG+TGG 0.484123 5_4:-20236392 None:intergenic
GATGTTCTTCTTGCAGGCAT+AGG 0.487244 5_4:+20238036 Msa0842840:CDS
CGGCGGTGGTGGCTGAGAAG+AGG 0.490749 5_4:-20236381 None:intergenic
TAAAGTTGAACATGGTAGAA+AGG 0.496799 5_4:-20240286 None:intergenic
CTTCTTCTCTACAAAATACA+TGG 0.501734 5_4:+20236544 Msa0842840:five_prime_UTR
ATACTTGCATCCTCTTTACA+TGG 0.501795 5_4:-20239839 None:intergenic
AAGGAAGCCGATTTCGGCGG+TGG 0.506754 5_4:-20236395 None:intergenic
TTGATTGGGCAGAGACTGCT+AGG 0.514063 5_4:+20237947 Msa0842840:intron
TGGCAAATGAATGGCAATGT+TGG 0.517863 5_4:+20240092 Msa0842840:three_prime_UTR
GAGTGGGAGAGAGGATAGAC+CGG 0.519188 5_4:-20236296 None:intergenic
GGCAAATGAATGGCAATGTT+GGG 0.524844 5_4:+20240093 Msa0842840:three_prime_UTR
CCACTGTCAGCATGAGAGAA+AGG 0.525406 5_4:-20240222 None:intergenic
TAAATACATCATATTCTGCT+TGG 0.529650 5_4:-20240036 None:intergenic
AAATGTGAATATGCAGCATT+GGG 0.532173 5_4:+20238089 Msa0842840:CDS
AACATACAAAAGGAGAAGAA+AGG 0.533617 5_4:+20239972 Msa0842840:three_prime_UTR
GAATCCTTCACACTATCTGC+AGG 0.534749 5_4:-20240062 None:intergenic
CTTAAATACTACTTGTTGGG+AGG 0.541392 5_4:+20240116 Msa0842840:three_prime_UTR
CTTCTTGCAGGCATAGGCAT+AGG 0.545790 5_4:+20238042 Msa0842840:CDS
ACATCCAATGAAATTGGAGG+TGG 0.549569 5_4:+20238012 Msa0842840:CDS
GGCAGAGACTGCTAGGATGT+TGG 0.549676 5_4:+20237954 Msa0842840:intron
GTGGTGGCTGAGAAGAGGGG+CGG 0.556578 5_4:-20236376 None:intergenic
ATAGGTCCCAAAGCCAAATG+AGG 0.563237 5_4:-20239896 None:intergenic
AGGGAGAATGACAAATGAGT+GGG 0.566247 5_4:-20236312 None:intergenic
GGCCCAATTAAGGTTAGTGG+AGG 0.566808 5_4:-20236249 None:intergenic
TCAGCAATATGGCAAATGAA+TGG 0.566898 5_4:+20240083 Msa0842840:three_prime_UTR
GTGATGATACTAAACTGAAG+TGG 0.566996 5_4:+20236713 Msa0842840:five_prime_UTR
CCTTTCTCTCATGCTGACAG+TGG 0.568781 5_4:+20240222 Msa0842840:three_prime_UTR
AGTGGGAGAGAGGATAGACC+GGG 0.569567 5_4:-20236295 None:intergenic
AGGATTAGAACCATGTAAAG+AGG 0.571409 5_4:+20239829 Msa0842840:intron
TGATGATACTAAACTGAAGT+GGG 0.584615 5_4:+20236714 Msa0842840:five_prime_UTR
AGCACCTGCAGATAGTGTGA+AGG 0.594876 5_4:+20240058 Msa0842840:three_prime_UTR
TATAAGGAAGCCGATTTCGG+CGG 0.596731 5_4:-20236398 None:intergenic
GATACATCCAATGAAATTGG+AGG 0.601573 5_4:+20238009 Msa0842840:CDS
GCGGTGGTGGCTGAGAAGAG+GGG 0.606814 5_4:-20236379 None:intergenic
GCATAGGCATAGGCAAGGCA+AGG 0.608211 5_4:+20238052 Msa0842840:CDS
TGCAGGCATAGGCATAGGCA+AGG 0.611887 5_4:+20238047 Msa0842840:CDS
GAGGGAGAATGACAAATGAG+TGG 0.613825 5_4:-20236313 None:intergenic
GTGAATATGCAGCATTGGGT+TGG 0.623077 5_4:+20238093 Msa0842840:CDS
GTGTTGAAAGCTTAAATCGA+TGG 0.630616 5_4:-20236226 None:intergenic
TAGGTCCCAAAGCCAAATGA+GGG 0.633837 5_4:-20239895 None:intergenic
GCCCAATTAAGGTTAGTGGA+GGG 0.641151 5_4:-20236248 None:intergenic
ACATGTAGTAAAGTTGAACA+TGG 0.650487 5_4:-20240294 None:intergenic
CAAAATCTCTAATCAAACCC+CGG 0.664906 5_4:+20236277 Msa0842840:five_prime_UTR
AAAGCCAAATGAGGGACCAG+AGG 0.666546 5_4:-20239887 None:intergenic
CTTCTCTACAAAATACATGG+AGG 0.693210 5_4:+20236547 Msa0842840:five_prime_UTR
TAGGCATAGGCAAGGCAAGG+AGG 0.705253 5_4:+20238055 Msa0842840:CDS
CTTCTCATATGTAATCACAG+AGG 0.763007 5_4:+20236759 Msa0842840:five_prime_UTR
GTGGGAGAGAGGATAGACCG+GGG 0.768528 5_4:-20236294 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAAAAAAAAATT+AGG - chr5_4:20238302-20238321 None:intergenic 0.0%
!! AAAAAAACATATACAAAAAT+TGG + chr5_4:20236445-20236464 Msa0842840:five_prime_UTR 10.0%
!! AAAAAACATATACAAAAATT+GGG + chr5_4:20236446-20236465 Msa0842840:five_prime_UTR 10.0%
!! TTAGATTAATTAACAAAAAA+AGG + chr5_4:20239026-20239045 Msa0842840:intron 10.0%
!! ATCTAATAACATAAAAAAAG+GGG - chr5_4:20236617-20236636 None:intergenic 15.0%
!! GATCTAATAACATAAAAAAA+GGG - chr5_4:20236618-20236637 None:intergenic 15.0%
!! AGATCTAATAACATAAAAAA+AGG - chr5_4:20236619-20236638 None:intergenic 15.0%
!!! GATTTTCTTTCAAAAAAAAA+AGG + chr5_4:20237040-20237059 Msa0842840:intron 15.0%
!! TTTAATTAGAGTTGAATAAT+AGG + chr5_4:20237083-20237102 Msa0842840:intron 15.0%
!!! TAATTATATGTTGAGAATTT+TGG + chr5_4:20237122-20237141 Msa0842840:intron 15.0%
!! TTACTTACATAAAATAAGTT+TGG - chr5_4:20237273-20237292 None:intergenic 15.0%
!! GTAAAAGAAAATAACAATAA+CGG + chr5_4:20237588-20237607 Msa0842840:intron 15.0%
!!! AAATTTTAATGTATGTGTTA+AGG - chr5_4:20237685-20237704 None:intergenic 15.0%
!! TCAAACTAGATAAAATAAAA+AGG - chr5_4:20237912-20237931 None:intergenic 15.0%
!! AAATTCTCAAAAAGATAAAA+AGG - chr5_4:20238959-20238978 None:intergenic 15.0%
!! AAGTTTAAATAATACTGAAA+TGG + chr5_4:20239318-20239337 Msa0842840:intron 15.0%
!! AGTTTAAATAATACTGAAAT+GGG + chr5_4:20239319-20239338 Msa0842840:intron 15.0%
!! TTTATTCTTATGTTAATGAT+AGG + chr5_4:20239809-20239828 Msa0842840:intron 15.0%
!!! ATAAATTTTGAACATACAAA+AGG + chr5_4:20239962-20239981 Msa0842840:three_prime_UTR 15.0%
!!! GTTTTTTTTATGATTCTGTT+CGG - chr5_4:20236433-20236452 None:intergenic 20.0%
!!! CTTTACATTTTTACATAGTT+TGG - chr5_4:20236582-20236601 None:intergenic 20.0%
!! TCTAATAACATAAAAAAAGG+GGG - chr5_4:20236616-20236635 None:intergenic 20.0%
!! TTAATGATATGTAGAAAAGT+AGG + chr5_4:20236942-20236961 Msa0842840:intron 20.0%
!! ACAAAATAAATAAGAACAGT+TGG - chr5_4:20236998-20237017 None:intergenic 20.0%
!!! TTATTTTGTGCAAATGTATT+TGG + chr5_4:20237009-20237028 Msa0842840:intron 20.0%
!! ATTAGAATATCGAATAAGTT+TGG - chr5_4:20237378-20237397 None:intergenic 20.0%
!! TATTCGATATTCTAATGAAT+TGG + chr5_4:20237382-20237401 Msa0842840:intron 20.0%
!! TATATGTGTTAAAAACTAAC+AGG - chr5_4:20237415-20237434 None:intergenic 20.0%
!! AAAAACATCTCTGATTATTA+CGG - chr5_4:20237636-20237655 None:intergenic 20.0%
!! AAAATAAAAAGGAAACTGAT+AGG - chr5_4:20237901-20237920 None:intergenic 20.0%
!!! ATTTTAGTAAGGTTTATGTA+AGG - chr5_4:20238377-20238396 None:intergenic 20.0%
!! TTGTTAAAAGGATATTCATT+TGG - chr5_4:20238586-20238605 None:intergenic 20.0%
!! TGTGTTATATGATTGTTAAA+AGG - chr5_4:20238598-20238617 None:intergenic 20.0%
!!! GCAATTAAATATTATCCTTT+TGG - chr5_4:20238803-20238822 None:intergenic 20.0%
!!! ATAAGTAGCATTTTTTTAGT+AGG + chr5_4:20238884-20238903 Msa0842840:intron 20.0%
!! AAGGATTCGATAAAAAAAAT+AGG - chr5_4:20238940-20238959 None:intergenic 20.0%
!! GTTTAAATAATACTGAAATG+GGG + chr5_4:20239320-20239339 Msa0842840:intron 20.0%
!!! ATGTTAATGAACCATTTTTT+TGG + chr5_4:20239772-20239791 Msa0842840:intron 20.0%
!! TTTATGTTTGTCCAAAAAAA+TGG - chr5_4:20239786-20239805 None:intergenic 20.0%
!! TTCAATGGGTTTAGTAATAT+CGG + chr5_4:20236691-20236710 Msa0842840:five_prime_UTR 25.0%
!! AAGTGGGATCTTATATTTTT+CGG + chr5_4:20236730-20236749 Msa0842840:five_prime_UTR 25.0%
!! ATATGTTGAGAATTTTGGAT+AGG + chr5_4:20237127-20237146 Msa0842840:intron 25.0%
!! GAATTTTGGATAGGAAAAAA+TGG + chr5_4:20237136-20237155 Msa0842840:intron 25.0%
! TCATGTTGAGATATAAATTG+AGG - chr5_4:20237227-20237246 None:intergenic 25.0%
! TAATTTCGATATATGCTTCA+AGG - chr5_4:20237305-20237324 None:intergenic 25.0%
! GACTTACGAAAAACTAAAAA+GGG - chr5_4:20237356-20237375 None:intergenic 25.0%
! AACTCATTTAAATGATTGCA+GGG + chr5_4:20237747-20237766 Msa0842840:intron 25.0%
! AGTCAAACATATCACAAAAA+GGG - chr5_4:20238165-20238184 None:intergenic 25.0%
! TAGTCAAACATATCACAAAA+AGG - chr5_4:20238166-20238185 None:intergenic 25.0%
! TTGATGTTGAGATCTAAATT+GGG - chr5_4:20238211-20238230 None:intergenic 25.0%
! TTTGATGTTGAGATCTAAAT+TGG - chr5_4:20238212-20238231 None:intergenic 25.0%
! GACTTACATAAAATGAAGTT+TGG - chr5_4:20238256-20238275 None:intergenic 25.0%
! AAAAATTAGGATATGCTTCA+AGG - chr5_4:20238289-20238308 None:intergenic 25.0%
! ATGAAGAAAAGTGATGTAAA+TGG - chr5_4:20238429-20238448 None:intergenic 25.0%
!! TCACTAAAAAATCAGTGTTT+TGG + chr5_4:20238528-20238547 Msa0842840:intron 25.0%
! ATTTAAAGATACCAAGAGAT+TGG - chr5_4:20238669-20238688 None:intergenic 25.0%
!! AAAAATGCTACTTATATGCT+TGG - chr5_4:20238879-20238898 None:intergenic 25.0%
!!! TTTTTAGTAGGATCATTTGA+TGG + chr5_4:20238896-20238915 Msa0842840:intron 25.0%
! TAAAAAAAATAGGCAAGTGA+GGG - chr5_4:20238930-20238949 None:intergenic 25.0%
! ATAAAAAAAATAGGCAAGTG+AGG - chr5_4:20238931-20238950 None:intergenic 25.0%
! GACATAACACAAGAAAAAAT+CGG - chr5_4:20239093-20239112 None:intergenic 25.0%
!!! TTTTTTCTTGTGTTATGTCT+AGG + chr5_4:20239094-20239113 Msa0842840:intron 25.0%
! TAATACAATTACACAAGCAA+AGG - chr5_4:20239163-20239182 None:intergenic 25.0%
! AATAACAACAATATTGAGTG+AGG + chr5_4:20239253-20239272 Msa0842840:intron 25.0%
! ATTTCTACCTACTTAACATT+TGG + chr5_4:20239385-20239404 Msa0842840:intron 25.0%
! TAAATACATCATATTCTGCT+TGG - chr5_4:20240039-20240058 None:intergenic 25.0%
!!! TGTTCGGTTATTTTGGTATA+AGG - chr5_4:20236417-20236436 None:intergenic 30.0%
! ATGATTCTGTTCGGTTATTT+TGG - chr5_4:20236424-20236443 None:intergenic 30.0%
! CATGTATTTTGTAGAGAAGA+AGG - chr5_4:20236546-20236565 None:intergenic 30.0%
CTTCTTCTCTACAAAATACA+TGG + chr5_4:20236544-20236563 Msa0842840:five_prime_UTR 30.0%
AAAAAAAGGGGGAAATAGTT+AGG - chr5_4:20236605-20236624 None:intergenic 30.0%
! TTGCTGTTAGATCTTTCAAT+GGG + chr5_4:20236677-20236696 Msa0842840:intron 30.0%
TGATGATACTAAACTGAAGT+GGG + chr5_4:20236714-20236733 Msa0842840:five_prime_UTR 30.0%
TTGTGCAAATGTATTTGGTT+AGG + chr5_4:20237014-20237033 Msa0842840:intron 30.0%
GATATAAATTGACGAAGTCT+TGG - chr5_4:20237188-20237207 None:intergenic 30.0%
GGACTTACGAAAAACTAAAA+AGG - chr5_4:20237357-20237376 None:intergenic 30.0%
CAAATATCTGAGTATCTGAT+AGG + chr5_4:20237502-20237521 Msa0842840:intron 30.0%
ATGTTTCATGATTTAGTGGT+TGG + chr5_4:20237536-20237555 Msa0842840:intron 30.0%
GAACTCATTTAAATGATTGC+AGG + chr5_4:20237746-20237765 Msa0842840:intron 30.0%
ACTCATTTAAATGATTGCAG+GGG + chr5_4:20237748-20237767 Msa0842840:intron 30.0%
TTGATATGTCTATTGTGAAC+TGG + chr5_4:20237788-20237807 Msa0842840:intron 30.0%
! TGAACTGGTTTACTATTTAC+TGG + chr5_4:20237803-20237822 Msa0842840:intron 30.0%
AAATGTGAATATGCAGCATT+GGG + chr5_4:20238089-20238108 Msa0842840:CDS 30.0%
TGATGTTGAGATCTAAATTG+GGG - chr5_4:20238210-20238229 None:intergenic 30.0%
! TTTTCAATCACCATGAATGA+AGG + chr5_4:20238325-20238344 Msa0842840:intron 30.0%
TAGTAAGGTTTATGTAAGGA+AGG - chr5_4:20238373-20238392 None:intergenic 30.0%
!!! GGGAAGGTATTATTTTAGTA+AGG - chr5_4:20238388-20238407 None:intergenic 30.0%
!! AAAAATCAGTGTTTTGGACT+TGG + chr5_4:20238534-20238553 Msa0842840:intron 30.0%
!! AAAATCAGTGTTTTGGACTT+GGG + chr5_4:20238535-20238554 Msa0842840:intron 30.0%
! TTTTCAGTGATGTAGATAGA+AGG - chr5_4:20238635-20238654 None:intergenic 30.0%
TCTATCTACATCACTGAAAA+TGG + chr5_4:20238635-20238654 Msa0842840:intron 30.0%
! AGATATTCAAGATTTTGAGC+TGG + chr5_4:20239582-20239601 Msa0842840:intron 30.0%
! GATATTCAAGATTTTGAGCT+GGG + chr5_4:20239583-20239602 Msa0842840:intron 30.0%
!! TTTTTCTCCTTTGAGTTATG+TGG - chr5_4:20239684-20239703 None:intergenic 30.0%
TGTAAATCTGTGTTTGAACA+GGG - chr5_4:20239709-20239728 None:intergenic 30.0%
TCATCTTTGTCCTATTCTAT+AGG - chr5_4:20239917-20239936 None:intergenic 30.0%
ACAAAGATGATGCAAGAAAT+TGG + chr5_4:20239927-20239946 Msa0842840:three_prime_UTR 30.0%
AACATACAAAAGGAGAAGAA+AGG + chr5_4:20239972-20239991 Msa0842840:three_prime_UTR 30.0%
AAAGGAAAAACAACTCATGA+AGG + chr5_4:20239990-20240009 Msa0842840:three_prime_UTR 30.0%
TAAAGTTGAACATGGTAGAA+AGG - chr5_4:20240289-20240308 None:intergenic 30.0%
ACATGTAGTAAAGTTGAACA+TGG - chr5_4:20240297-20240316 None:intergenic 30.0%
GTGTTGAAAGCTTAAATCGA+TGG - chr5_4:20236229-20236248 None:intergenic 35.0%
! TTGATTAGAGATTTTGGGCT+TGG - chr5_4:20236273-20236292 None:intergenic 35.0%
! GGGGTTTGATTAGAGATTTT+GGG - chr5_4:20236278-20236297 None:intergenic 35.0%
CAAAATCTCTAATCAAACCC+CGG + chr5_4:20236277-20236296 Msa0842840:five_prime_UTR 35.0%
CTTCTCTACAAAATACATGG+AGG + chr5_4:20236547-20236566 Msa0842840:five_prime_UTR 35.0%
CTAACAGCAACAATGAAAAC+AGG - chr5_4:20236667-20236686 None:intergenic 35.0%
! GTTGCTGTTAGATCTTTCAA+TGG + chr5_4:20236676-20236695 Msa0842840:intron 35.0%
GTGATGATACTAAACTGAAG+TGG + chr5_4:20236713-20236732 Msa0842840:five_prime_UTR 35.0%
CTTCTCATATGTAATCACAG+AGG + chr5_4:20236759-20236778 Msa0842840:five_prime_UTR 35.0%
AAATTGAGGAAGACAAGTTG+AGG - chr5_4:20237213-20237232 None:intergenic 35.0%
! ATACTCCAACAAGTTGAAAG+TGG - chr5_4:20237453-20237472 None:intergenic 35.0%
GTGGATGTTTCATGATTTAG+TGG + chr5_4:20237532-20237551 Msa0842840:intron 35.0%
! ACTTTCTTTGAAAATGCCTC+TGG + chr5_4:20237859-20237878 Msa0842840:intron 35.0%
AAAACAGAAACAAAGTCCAG+AGG - chr5_4:20237878-20237897 None:intergenic 35.0%
! ATGTCTGAAGAAGCTTTGAT+TGG + chr5_4:20237932-20237951 Msa0842840:intron 35.0%
! TGTCTGAAGAAGCTTTGATT+GGG + chr5_4:20237933-20237952 Msa0842840:intron 35.0%
GTGGATACATCCAATGAAAT+TGG + chr5_4:20238006-20238025 Msa0842840:CDS 35.0%
GATACATCCAATGAAATTGG+AGG + chr5_4:20238009-20238028 Msa0842840:CDS 35.0%
CAATGCTGCATATTCACATT+TGG - chr5_4:20238090-20238109 None:intergenic 35.0%
CAAATGTGAATATGCAGCAT+TGG + chr5_4:20238088-20238107 Msa0842840:CDS 35.0%
TGGTACTAATCTCACTCATT+TGG + chr5_4:20238113-20238132 Msa0842840:intron 35.0%
! GTGATGTAAATGGTGATGAA+GGG - chr5_4:20238419-20238438 None:intergenic 35.0%
! AGTGATGTAAATGGTGATGA+AGG - chr5_4:20238420-20238439 None:intergenic 35.0%
!! AAATCAGTGTTTTGGACTTG+GGG + chr5_4:20238536-20238555 Msa0842840:intron 35.0%
! TTTGGCATCTGTGATTTTGA+GGG - chr5_4:20238568-20238587 None:intergenic 35.0%
! ATTTGGCATCTGTGATTTTG+AGG - chr5_4:20238569-20238588 None:intergenic 35.0%
TGGTGATAATACCAATCTCT+TGG + chr5_4:20238655-20238674 Msa0842840:intron 35.0%
TTTAGGTCTCAACATCCAAA+AGG + chr5_4:20238785-20238804 Msa0842840:intron 35.0%
ACAACAATATTGAGTGAGGA+TGG + chr5_4:20239257-20239276 Msa0842840:intron 35.0%
CAACAATATTGAGTGAGGAT+GGG + chr5_4:20239258-20239277 Msa0842840:intron 35.0%
CTTCAAGCCAAATGTTAAGT+AGG - chr5_4:20239395-20239414 None:intergenic 35.0%
TTTGCTTCCACATAACTCAA+AGG + chr5_4:20239674-20239693 Msa0842840:intron 35.0%
GTGTAAATCTGTGTTTGAAC+AGG - chr5_4:20239710-20239729 None:intergenic 35.0%
AGGATTAGAACCATGTAAAG+AGG + chr5_4:20239829-20239848 Msa0842840:intron 35.0%
ATACTTGCATCCTCTTTACA+TGG - chr5_4:20239842-20239861 None:intergenic 35.0%
! AAAGAGGATGCAAGTATTGT+TGG + chr5_4:20239845-20239864 Msa0842840:CDS 35.0%
TCAGCAATATGGCAAATGAA+TGG + chr5_4:20240083-20240102 Msa0842840:three_prime_UTR 35.0%
TGGGCTTAAATACTACTTGT+TGG + chr5_4:20240112-20240131 Msa0842840:three_prime_UTR 35.0%
GGGCTTAAATACTACTTGTT+GGG + chr5_4:20240113-20240132 Msa0842840:three_prime_UTR 35.0%
! CTTAAATACTACTTGTTGGG+AGG + chr5_4:20240116-20240135 Msa0842840:three_prime_UTR 35.0%
ACCCTCCACTAACCTTAATT+GGG + chr5_4:20236247-20236266 Msa0842840:five_prime_UTR 40.0%
! CGGGGTTTGATTAGAGATTT+TGG - chr5_4:20236279-20236298 None:intergenic 40.0%
AGGGAGAATGACAAATGAGT+GGG - chr5_4:20236315-20236334 None:intergenic 40.0%
!!! GTGTGTGTTTTAGGGTTTGA+GGG - chr5_4:20236334-20236353 None:intergenic 40.0%
! GGAAACTGGTGTGTGTTTTA+GGG - chr5_4:20236342-20236361 None:intergenic 40.0%
! TGGTATAAGGAAGCCGATTT+CGG - chr5_4:20236404-20236423 None:intergenic 40.0%
AGCTACCACTTTCAACTTGT+TGG + chr5_4:20237445-20237464 Msa0842840:intron 40.0%
GTATCTGATAGGAAAATGCG+TGG + chr5_4:20237513-20237532 Msa0842840:intron 40.0%
ACATCCAATGAAATTGGAGG+TGG + chr5_4:20238012-20238031 Msa0842840:CDS 40.0%
ATCTCCACCTCCAATTTCAT+TGG - chr5_4:20238019-20238038 None:intergenic 40.0%
GTTGAGATCTAAATTGGGGA+AGG - chr5_4:20238206-20238225 None:intergenic 40.0%
ACAATGCACTCCTTCATTCA+TGG - chr5_4:20238338-20238357 None:intergenic 40.0%
ATGAAGGGTTTATACAGGGA+AGG - chr5_4:20238404-20238423 None:intergenic 40.0%
GGTGATGAAGGGTTTATACA+GGG - chr5_4:20238408-20238427 None:intergenic 40.0%
! TGGTGATGAAGGGTTTATAC+AGG - chr5_4:20238409-20238428 None:intergenic 40.0%
!! ACACCTAGAGTGCTCATTTT+AGG + chr5_4:20238768-20238787 Msa0842840:intron 40.0%
AGACCTAAAATGAGCACTCT+AGG - chr5_4:20238774-20238793 None:intergenic 40.0%
! TAGTAGGATCATTTGATGGC+TGG + chr5_4:20238900-20238919 Msa0842840:intron 40.0%
AAAAAATAGGCAAGTGAGGG+AGG - chr5_4:20238927-20238946 None:intergenic 40.0%
GGGAGATTAGAATGCATTGA+AGG + chr5_4:20239603-20239622 Msa0842840:intron 40.0%
GAATGCATTGAAGGAGTTGT+TGG + chr5_4:20239612-20239631 Msa0842840:intron 40.0%
TCAAAGTTTCCCAGTGTGTT+TGG - chr5_4:20239638-20239657 None:intergenic 40.0%
ACACTGGGAAACTTTGATGA+AGG + chr5_4:20239641-20239660 Msa0842840:intron 40.0%
GTGTGAAGGATTCAGCAATA+TGG + chr5_4:20240072-20240091 Msa0842840:three_prime_UTR 40.0%
TGGCAAATGAATGGCAATGT+TGG + chr5_4:20240092-20240111 Msa0842840:three_prime_UTR 40.0%
GGCAAATGAATGGCAATGTT+GGG + chr5_4:20240093-20240112 Msa0842840:three_prime_UTR 40.0%
!!! TCCTTTTTTCATGCTGACAG+TGG - chr5_4:20240383-20240402 None:intergenic 40.0%
CACCCTCCACTAACCTTAAT+TGG + chr5_4:20236246-20236265 Msa0842840:five_prime_UTR 45.0%
GCCCAATTAAGGTTAGTGGA+GGG - chr5_4:20236251-20236270 None:intergenic 45.0%
CTTGGCCCAATTAAGGTTAG+TGG - chr5_4:20236255-20236274 None:intergenic 45.0%
! TTTTGGGCTTGGCCCAATTA+AGG - chr5_4:20236262-20236281 None:intergenic 45.0%
ATGACAAATGAGTGGGAGAG+AGG - chr5_4:20236308-20236327 None:intergenic 45.0%
GAGGGAGAATGACAAATGAG+TGG - chr5_4:20236316-20236335 None:intergenic 45.0%
!!! GGTGTGTGTTTTAGGGTTTG+AGG - chr5_4:20236335-20236354 None:intergenic 45.0%
! GGGAAACTGGTGTGTGTTTT+AGG - chr5_4:20236343-20236362 None:intergenic 45.0%
! TATAAGGAAGCCGATTTCGG+CGG - chr5_4:20236401-20236420 None:intergenic 45.0%
GATGTTCTTCTTGCAGGCAT+AGG + chr5_4:20238036-20238055 Msa0842840:CDS 45.0%
! GTGAATATGCAGCATTGGGT+TGG + chr5_4:20238093-20238112 Msa0842840:CDS 45.0%
! AGAGTGCTCAGTTTAGTGCT+CGG + chr5_4:20238838-20238857 Msa0842840:intron 45.0%
AGTTGTTGGCCAAACACACT+GGG + chr5_4:20239626-20239645 Msa0842840:intron 45.0%
GGAAACTTTGATGAAGGAGC+TGG + chr5_4:20239647-20239666 Msa0842840:intron 45.0%
TAGGTCCCAAAGCCAAATGA+GGG - chr5_4:20239898-20239917 None:intergenic 45.0%
ATAGGTCCCAAAGCCAAATG+AGG - chr5_4:20239899-20239918 None:intergenic 45.0%
!! GGCTTTGGGACCTATAGAAT+AGG + chr5_4:20239904-20239923 Msa0842840:CDS 45.0%
GAATCCTTCACACTATCTGC+AGG - chr5_4:20240065-20240084 None:intergenic 45.0%
! ATGCTGACAGTGGCTTTTGT+GGG + chr5_4:20240232-20240251 Msa0842840:three_prime_UTR 45.0%
GCCACTGTCAGCATGAAAAA+AGG + chr5_4:20240379-20240398 Msa0842840:three_prime_UTR 45.0%
GGCCCAATTAAGGTTAGTGG+AGG - chr5_4:20236252-20236271 None:intergenic 50.0%
AGAATCCTGCTCTTCGCATC+AGG + chr5_4:20237474-20237493 Msa0842840:intron 50.0%
TTGATTGGGCAGAGACTGCT+AGG + chr5_4:20237947-20237966 Msa0842840:intron 50.0%
CATGAAGCACGTAGCGATAG+TGG + chr5_4:20237987-20238006 Msa0842840:CDS 50.0%
GGTGGAGATGTTCTTCTTGC+AGG + chr5_4:20238030-20238049 Msa0842840:CDS 50.0%
CTTCTTGCAGGCATAGGCAT+AGG + chr5_4:20238042-20238061 Msa0842840:CDS 50.0%
GAGTTGTTGGCCAAACACAC+TGG + chr5_4:20239625-20239644 Msa0842840:intron 50.0%
!! TGTTGGCTCTTCATTGCTGC+TGG + chr5_4:20239862-20239881 Msa0842840:CDS 50.0%
!! TTCATTGCTGCTGGTTCCTC+TGG + chr5_4:20239871-20239890 Msa0842840:CDS 50.0%
AAAGCCAAATGAGGGACCAG+AGG - chr5_4:20239890-20239909 None:intergenic 50.0%
! TCTGGTCCCTCATTTGGCTT+TGG + chr5_4:20239889-20239908 Msa0842840:CDS 50.0%
!! CTGGTCCCTCATTTGGCTTT+GGG + chr5_4:20239890-20239909 Msa0842840:CDS 50.0%
AGCACCTGCAGATAGTGTGA+AGG + chr5_4:20240058-20240077 Msa0842840:three_prime_UTR 50.0%
CCACTGTCAGCATGAGAGAA+AGG - chr5_4:20240225-20240244 None:intergenic 50.0%
CCTTTCTCTCATGCTGACAG+TGG + chr5_4:20240222-20240241 Msa0842840:three_prime_UTR 50.0%
! CATGCTGACAGTGGCTTTTG+TGG + chr5_4:20240231-20240250 Msa0842840:three_prime_UTR 50.0%
! GCGGCTGTTACAGCTTTTGT+GGG - chr5_4:20240412-20240431 None:intergenic 50.0%
AGTGGGAGAGAGGATAGACC+GGG - chr5_4:20236298-20236317 None:intergenic 55.0%
GAGTGGGAGAGAGGATAGAC+CGG - chr5_4:20236299-20236318 None:intergenic 55.0%
CATCACCTGATGCGAAGAGC+AGG - chr5_4:20237482-20237501 None:intergenic 55.0%
GGCAGAGACTGCTAGGATGT+TGG + chr5_4:20237954-20237973 Msa0842840:intron 55.0%
TGCAGGCATAGGCATAGGCA+AGG + chr5_4:20238047-20238066 Msa0842840:CDS 55.0%
GCATAGGCATAGGCAAGGCA+AGG + chr5_4:20238052-20238071 Msa0842840:CDS 55.0%
TAGGCATAGGCAAGGCAAGG+AGG + chr5_4:20238055-20238074 Msa0842840:CDS 55.0%
GGTTCCTCTGGTCCCTCATT+TGG + chr5_4:20239883-20239902 Msa0842840:CDS 55.0%
GTGGGAGAGAGGATAGACCG+GGG - chr5_4:20236297-20236316 None:intergenic 60.0%
TCAGCCACCACCGCCGAAAT+CGG + chr5_4:20236388-20236407 Msa0842840:five_prime_UTR 60.0%
! AAGGAAGCCGATTTCGGCGG+TGG - chr5_4:20236398-20236417 None:intergenic 60.0%
GTGGTGGCTGAGAAGAGGGG+CGG - chr5_4:20236379-20236398 None:intergenic 65.0%
! GCGGTGGTGGCTGAGAAGAG+GGG - chr5_4:20236382-20236401 None:intergenic 65.0%
! GGCGGTGGTGGCTGAGAAGA+GGG - chr5_4:20236383-20236402 None:intergenic 65.0%
! GAAGCCGATTTCGGCGGTGG+TGG - chr5_4:20236395-20236414 None:intergenic 65.0%
TGGCGGCGGTTGGGGGAAAC+TGG - chr5_4:20236356-20236375 None:intergenic 70.0%
GCTGAGAAGAGGGGCGGTGG+CGG - chr5_4:20236373-20236392 None:intergenic 70.0%
GTGGCTGAGAAGAGGGGCGG+TGG - chr5_4:20236376-20236395 None:intergenic 70.0%
! CGGCGGTGGTGGCTGAGAAG+AGG - chr5_4:20236384-20236403 None:intergenic 70.0%
! GAGAAGAGGGGCGGTGGCGG+CGG - chr5_4:20236370-20236389 None:intergenic 75.0%
!! AGGGGCGGTGGCGGCGGTTG+GGG - chr5_4:20236364-20236383 None:intergenic 80.0%
!! GAGGGGCGGTGGCGGCGGTT+GGG - chr5_4:20236365-20236384 None:intergenic 80.0%
!! AGAGGGGCGGTGGCGGCGGT+TGG - chr5_4:20236366-20236385 None:intergenic 80.0%
!! GGGGCGGTGGCGGCGGTTGG+GGG - chr5_4:20236363-20236382 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 20236213 20240431 20236213 ID=Msa0842840;Name=Msa0842840
chr5_4 mRNA 20236213 20240431 20236213 ID=Msa0842840-mRNA-1;Parent=Msa0842840;Name=Msa0842840-mRNA-1;_AED=0.08;_eAED=0.08;_QI=488|0.66|1|1|0|0|4|506|79
chr5_4 exon 20236213 20236595 20236213 ID=Msa0842840-mRNA-1:exon:8350;Parent=Msa0842840-mRNA-1
chr5_4 exon 20236687 20236780 20236687 ID=Msa0842840-mRNA-1:exon:8351;Parent=Msa0842840-mRNA-1
chr5_4 exon 20237959 20238114 20237959 ID=Msa0842840-mRNA-1:exon:8352;Parent=Msa0842840-mRNA-1
chr5_4 exon 20239831 20240431 20239831 ID=Msa0842840-mRNA-1:exon:8353;Parent=Msa0842840-mRNA-1
chr5_4 five_prime_UTR 20236213 20236595 20236213 ID=Msa0842840-mRNA-1:five_prime_utr;Parent=Msa0842840-mRNA-1
chr5_4 five_prime_UTR 20236687 20236780 20236687 ID=Msa0842840-mRNA-1:five_prime_utr;Parent=Msa0842840-mRNA-1
chr5_4 five_prime_UTR 20237959 20237969 20237959 ID=Msa0842840-mRNA-1:five_prime_utr;Parent=Msa0842840-mRNA-1
chr5_4 CDS 20237970 20238114 20237970 ID=Msa0842840-mRNA-1:cds;Parent=Msa0842840-mRNA-1
chr5_4 CDS 20239831 20239925 20239831 ID=Msa0842840-mRNA-1:cds;Parent=Msa0842840-mRNA-1
chr5_4 three_prime_UTR 20239926 20240431 20239926 ID=Msa0842840-mRNA-1:three_prime_utr;Parent=Msa0842840-mRNA-1
Gene Sequence

>Msa0842840

ATGTTGGCAGATGAGTTCATGAAGCACGTAGCGATAGTGGATACATCCAATGAAATTGGAGGTGGAGATGTTCTTCTTGCAGGCATAGGCATAGGCAAGGCAAGGAGGATGCAAGTTCCAAATGTGAATATGCAGCATTGGGTTGGATTAGAACCATGTAAAGAGGATGCAAGTATTGTTGGCTCTTCATTGCTGCTGGTTCCTCTGGTCCCTCATTTGGCTTTGGGACCTATAGAATAG
Protein sequence

>Msa0842840

MLADEFMKHVAIVDTSNEIGGGDVLLAGIGIGKARRMQVPNVNMQHWVGLEPCKEDASIVGSSLLLVPLVPHLALGPIE*