AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0843350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843350 MtrunA17_Chr5g0414051 95.612 433 19 0 1 433 1 433 0.0 820
Msa0843350 MtrunA17_Chr5g0414091 66.744 433 123 6 7 424 9 435 0.0 557
Msa0843350 MtrunA17_Chr8g0391481 67.663 368 114 3 58 421 7 373 0.0 533
Msa0843350 MtrunA17_Chr5g0414061 61.149 435 142 7 5 424 20 442 0.0 521
Msa0843350 MtrunA17_Chr5g0414041 61.298 447 150 6 1 433 7 444 0.0 518
Msa0843350 MtrunA17_Chr3g0140371 68.627 357 109 3 66 422 9 362 0.0 514
Msa0843350 MtrunA17_Chr7g0226991 66.106 357 119 2 67 422 49 404 1.04e-178 504
Msa0843350 MtrunA17_Chr5g0414081 72.000 200 55 1 223 422 3 201 5.54e-108 317
Msa0843350 MtrunA17_Chr8g0387921 39.614 414 230 11 19 420 14 419 4.33e-97 297
Msa0843350 MtrunA17_Chr6g0456691 38.480 421 230 12 14 421 16 420 1.12e-94 291
Msa0843350 MtrunA17_Chr8g0347321 38.776 392 220 9 41 420 56 439 6.58e-94 290
Msa0843350 MtrunA17_Chr6g0456681 38.519 405 222 12 27 421 22 409 1.03e-85 268
Msa0843350 MtrunA17_Chr6g0451321 34.305 446 238 14 17 422 29 459 2.08e-81 258
Msa0843350 MtrunA17_Chr2g0283851 37.396 361 194 14 78 422 58 402 1.26e-75 241
Msa0843350 MtrunA17_Chr1g0188191 34.626 361 212 10 75 422 234 583 5.11e-67 224
Msa0843350 MtrunA17_Chr6g0476041 33.795 361 215 10 75 422 167 516 1.08e-63 213
Msa0843350 MtrunA17_Chr7g0259711 33.780 373 193 18 75 422 91 434 1.01e-52 182
Msa0843350 MtrunA17_Chr1g0175671 34.605 367 197 15 75 422 170 512 2.79e-51 181
Msa0843350 MtrunA17_Chr2g0287901 32.698 367 208 10 75 424 97 441 2.83e-49 174
Msa0843350 MtrunA17_Chr7g0258851 33.598 378 206 14 75 431 248 601 6.68e-49 176
Msa0843350 MtrunA17_Chr7g0221281 36.437 247 140 9 180 421 3 237 1.70e-47 164
Msa0843350 MtrunA17_Chr1g0213281 31.266 403 217 18 60 431 155 528 5.49e-45 164
Msa0843350 MtrunA17_Chr4g0071391 31.880 367 200 13 75 420 109 446 1.31e-44 161
Msa0843350 MtrunA17_Chr4g0006991 31.481 378 213 17 64 420 75 427 2.70e-44 159
Msa0843350 MtrunA17_Chr8g0384151 30.726 358 205 12 75 420 90 416 3.00e-44 159
Msa0843350 MtrunA17_Chr3g0105301 32.022 356 210 10 71 416 39 372 6.00e-43 155
Msa0843350 MtrunA17_Chr5g0430371 30.303 363 223 10 67 416 50 395 5.24e-42 153
Msa0843350 MtrunA17_Chr7g0269991 30.919 359 201 13 75 417 137 464 1.20e-41 153
Msa0843350 MtrunA17_Chr3g0126021 29.195 435 250 16 10 420 59 459 4.35e-41 152
Msa0843350 MtrunA17_Chr8g0368461 29.306 389 209 16 75 426 178 537 1.05e-40 152
Msa0843350 MtrunA17_Chr5g0413271 29.945 364 220 13 69 422 140 478 1.66e-40 150
Msa0843350 MtrunA17_Chr4g0070341 28.571 371 209 10 75 420 132 471 3.70e-40 149
Msa0843350 MtrunA17_Chr1g0171171 27.612 402 247 11 29 420 8 375 5.91e-40 147
Msa0843350 MtrunA17_Chr8g0360521 29.144 374 212 14 72 422 68 411 1.23e-39 147
Msa0843350 MtrunA17_Chr7g0242701 29.659 381 216 17 64 420 75 427 3.26e-39 146
Msa0843350 MtrunA17_Chr3g0113671 28.809 361 223 13 72 416 103 445 6.25e-39 145
Msa0843350 MtrunA17_Chr4g0068111 30.053 376 205 13 71 420 110 453 1.81e-38 144
Msa0843350 MtrunA17_Chr2g0325481 32.102 352 188 15 75 415 50 361 2.03e-38 142
Msa0843350 MtrunA17_Chr8g0384421 27.467 375 208 12 75 420 133 472 2.24e-38 144
Msa0843350 MtrunA17_Chr7g0221271 35.870 184 109 2 24 198 22 205 1.06e-37 136
Msa0843350 MtrunA17_Chr3g0097891 30.659 349 202 10 75 416 31 346 3.20e-37 139
Msa0843350 MtrunA17_Chr3g0085081 31.054 351 196 16 75 416 31 344 1.14e-36 137
Msa0843350 MtrunA17_Chr3g0091031 30.484 351 198 16 75 416 31 344 1.87e-36 137
Msa0843350 MtrunA17_Chr4g0005491 28.406 433 263 19 1 416 1 403 3.65e-36 137
Msa0843350 MtrunA17_Chr3g0085071 31.054 351 196 15 75 416 46 359 3.88e-36 136
Msa0843350 MtrunA17_Chr4g0041011 29.975 407 228 18 24 420 4 363 9.89e-36 135
Msa0843350 MtrunA17_Chr7g0243301 27.512 418 241 18 23 416 26 405 6.36e-35 134
Msa0843350 MtrunA17_Chr6g0455901 26.941 438 245 17 22 420 15 416 3.81e-34 132
Msa0843350 MtrunA17_Chr2g0314801 30.056 356 210 14 72 420 30 353 4.50e-34 130
Msa0843350 MtrunA17_Chr4g0045951 29.793 386 227 14 34 413 8 355 1.01e-33 129
Msa0843350 MtrunA17_Chr3g0114101 27.763 371 208 16 75 417 89 427 2.54e-33 130
Msa0843350 MtrunA17_Chr5g0437601 26.486 370 220 15 72 417 80 421 4.40e-33 129
Msa0843350 MtrunA17_Chr2g0314791 29.041 365 198 15 72 420 26 345 9.91e-33 126
Msa0843350 MtrunA17_Chr5g0413851 30.748 361 200 14 67 416 34 355 1.72e-32 126
Msa0843350 MtrunA17_Chr1g0210771 27.793 367 193 15 75 416 59 378 1.08e-31 124
Msa0843350 MtrunA17_Chr4g0045941 25.971 412 221 14 17 413 1 343 3.84e-31 122
Msa0843350 MtrunA17_Chr2g0283581 28.040 403 228 17 30 420 6 358 6.34e-31 122
Msa0843350 MtrunA17_Chr5g0437291 27.945 365 218 17 75 416 165 507 2.75e-30 122
Msa0843350 MtrunA17_Chr7g0221291 35.625 160 93 2 13 166 15 170 3.21e-30 115
Msa0843350 MtrunA17_Chr4g0005481 28.267 375 216 16 69 420 102 446 3.32e-30 121
Msa0843350 MtrunA17_Chr4g0049421 26.596 376 215 13 75 420 48 392 8.42e-27 111
Msa0843350 MtrunA17_Chr1g0178081 32.411 253 149 10 23 259 5 251 1.78e-25 106
Msa0843350 MtrunA17_Chr1g0178081 34.783 115 61 5 310 422 242 344 4.28e-12 67.4
Msa0843350 MtrunA17_Chr1g0209921 44.554 101 54 2 68 166 37 137 4.63e-22 95.1
Msa0843350 MtrunA17_Chr7g0275501 55.738 61 24 1 359 419 11 68 1.60e-16 73.9
Msa0843350 MtrunA17_Chr2g0287911 46.429 56 23 1 75 130 61 109 1.57e-11 60.8
Msa0843350 MtrunA17_Chr4g0057171 40.217 92 46 3 213 304 1 83 9.71e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843350 AT1G70230.1 48.077 416 183 10 23 423 17 414 8.36e-135 394
Msa0843350 AT4G23790.1 37.819 431 247 11 5 420 3 427 1.18e-93 289
Msa0843350 AT1G01430.1 40.106 379 205 11 54 420 79 447 5.30e-93 288
Msa0843350 AT4G01080.1 39.286 392 222 8 32 418 48 428 5.79e-93 288
Msa0843350 AT5G15890.1 39.578 379 208 11 47 421 162 523 7.82e-91 285
Msa0843350 AT4G11090.1 38.824 425 229 13 17 420 11 425 1.36e-89 278
Msa0843350 AT3G02440.1 39.665 358 200 9 75 426 127 474 7.95e-88 275
Msa0843350 AT3G02440.2 39.665 358 200 9 75 426 135 482 9.39e-88 276
Msa0843350 AT3G28150.1 36.870 377 210 10 60 422 50 412 7.60e-86 268
Msa0843350 AT5G15900.1 37.293 362 206 11 75 427 67 416 2.05e-81 257
Msa0843350 AT5G15900.2 37.293 362 206 11 75 427 86 435 2.42e-81 258
Msa0843350 AT4G25360.1 33.607 366 217 9 72 422 172 526 2.03e-63 213
Msa0843350 AT4G25360.2 33.607 366 217 9 72 422 172 526 2.03e-63 213
Msa0843350 AT5G51640.1 32.124 386 214 12 72 431 135 498 1.82e-61 207
Msa0843350 AT5G19160.1 30.022 463 277 14 3 431 6 455 2.64e-54 187
Msa0843350 AT5G06700.1 33.784 370 196 16 75 422 255 597 1.30e-49 178
Msa0843350 AT3G06080.2 30.997 371 207 10 75 422 103 447 1.60e-48 172
Msa0843350 AT3G12060.1 32.095 377 209 15 75 430 196 546 5.38e-48 172
Msa0843350 AT2G30010.1 30.867 392 241 11 41 420 23 396 2.70e-47 167
Msa0843350 AT5G64020.1 30.563 373 211 12 75 420 57 408 2.76e-47 167
Msa0843350 AT3G11570.1 30.641 421 239 16 22 420 30 419 6.71e-47 167
Msa0843350 AT2G30010.2 30.867 392 241 11 41 420 66 439 6.88e-47 167
Msa0843350 AT1G48880.1 33.978 362 190 15 70 417 110 436 1.92e-45 163
Msa0843350 AT5G20590.1 32.692 364 194 14 75 416 141 475 2.30e-43 159
Msa0843350 AT5G58600.1 30.641 359 212 11 75 420 66 400 1.34e-42 155
Msa0843350 AT5G06230.2 31.806 371 201 13 75 420 21 364 2.32e-42 153
Msa0843350 AT5G06230.1 31.806 371 201 13 75 420 62 405 6.14e-42 153
Msa0843350 AT3G62390.1 30.108 372 211 16 69 422 134 474 7.09e-42 154
Msa0843350 AT5G49340.1 27.747 364 223 9 75 420 100 441 9.73e-42 154
Msa0843350 AT2G14530.1 30.769 377 198 17 75 420 62 406 1.37e-41 152
Msa0843350 AT2G40160.1 29.439 428 252 20 19 416 12 419 3.21e-40 149
Msa0843350 AT2G40160.2 29.439 428 252 20 19 416 12 419 5.10e-40 149
Msa0843350 AT5G01360.2 28.455 369 231 14 64 417 25 375 9.91e-40 146
Msa0843350 AT5G20680.6 27.154 383 211 10 69 420 112 457 2.10e-39 147
Msa0843350 AT2G42570.1 31.335 367 198 16 67 420 39 364 2.40e-39 145
Msa0843350 AT5G20680.4 27.034 381 214 9 69 420 206 551 2.94e-39 149
Msa0843350 AT5G20680.1 27.034 381 214 9 69 420 206 551 2.94e-39 149
Msa0843350 AT5G20680.3 27.034 381 214 9 69 420 206 551 2.94e-39 149
Msa0843350 AT5G20680.5 27.034 381 214 9 69 420 206 551 2.94e-39 149
Msa0843350 AT5G20680.2 27.034 381 214 9 69 420 188 533 3.07e-39 148
Msa0843350 AT5G01360.1 28.455 369 231 14 64 417 81 431 3.82e-39 146
Msa0843350 AT2G37720.1 28.010 382 207 9 70 420 138 482 4.11e-39 147
Msa0843350 AT1G73140.1 28.877 374 216 15 64 416 57 401 5.53e-39 145
Msa0843350 AT1G60790.1 27.056 377 231 14 66 421 181 534 2.11e-38 146
Msa0843350 AT3G55990.1 30.053 376 219 18 63 416 126 479 2.37e-38 145
Msa0843350 AT2G40150.1 29.508 366 214 16 75 416 71 416 8.10e-38 142
Msa0843350 AT5G19160.2 29.706 340 196 11 3 309 6 335 3.08e-36 136
Msa0843350 AT2G38320.1 26.942 412 261 15 20 416 14 400 3.96e-36 137
Msa0843350 AT3G11030.1 28.533 368 216 15 75 420 106 448 8.87e-36 137
Msa0843350 AT2G31110.2 29.700 367 200 16 65 416 35 358 9.67e-36 135
Msa0843350 AT2G30900.1 28.693 352 208 13 75 416 43 361 2.31e-35 134
Msa0843350 AT3G14850.2 31.198 359 196 15 75 420 34 354 4.71e-35 133
Msa0843350 AT1G29050.1 28.997 369 212 15 63 420 49 378 1.17e-34 133
Msa0843350 AT5G64470.3 28.108 370 214 15 74 420 54 394 4.00e-34 132
Msa0843350 AT2G40320.1 27.198 364 225 13 72 417 78 419 9.88e-34 131
Msa0843350 AT5G64470.2 27.568 370 222 15 74 420 54 400 1.18e-32 128
Msa0843350 AT3G54260.1 26.989 352 215 11 75 416 51 370 2.29e-31 124
Msa0843350 AT2G34070.1 28.455 369 207 15 67 420 57 383 4.65e-31 123
Msa0843350 AT3G06080.1 30.830 253 141 6 75 309 103 339 5.15e-31 122
Msa0843350 AT1G78710.1 29.539 369 208 15 64 420 28 356 4.52e-30 120
Msa0843350 AT3G62390.2 28.421 285 172 8 43 309 102 372 1.02e-29 120
Msa0843350 AT2G40160.3 29.126 309 190 12 19 308 12 310 1.04e-29 117
Msa0843350 AT2G38320.2 28.528 326 204 13 105 416 7 317 3.85e-29 116
Msa0843350 AT5G01620.2 26.087 368 230 12 71 420 106 449 8.22e-29 118
Msa0843350 AT5G01620.1 26.087 368 230 12 71 420 106 449 8.22e-29 118
Msa0843350 AT5G01620.3 26.301 365 233 12 71 420 114 457 1.20e-28 117
Msa0843350 AT5G20590.2 35.751 193 107 6 75 251 141 332 2.79e-28 115
Msa0843350 AT5G06230.3 31.621 253 145 7 75 309 62 304 3.96e-27 110
Msa0843350 AT5G58600.2 32.500 240 140 8 75 308 66 289 4.73e-27 110
Msa0843350 AT2G30900.2 51.111 90 43 1 75 163 43 132 1.77e-24 102
Msa0843350 AT2G31110.3 30.526 190 113 5 65 246 35 213 9.64e-23 97.4
Msa0843350 AT1G78710.2 45.133 113 53 4 57 163 9 118 9.26e-21 92.4
Msa0843350 AT5G64470.1 26.389 288 171 12 74 338 54 323 5.34e-20 90.9
Msa0843350 AT5G64470.4 27.372 274 158 12 74 324 54 309 3.32e-19 88.6
Msa0843350 AT3G14850.1 27.778 288 157 15 146 420 2 251 6.59e-12 65.9

Find 124 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAATTTCTTGATGATTT+TGG 0.053119 5_4:-21001429 None:intergenic
GTCCTTAAAGCCTTTCTTAA+TGG 0.091385 5_4:-21003573 None:intergenic
TTCCATTAAGAAAGGCTTTA+AGG 0.121836 5_4:+21003571 Msa0843350:CDS
AAATGTATTGTATTAAAATT+TGG 0.148603 5_4:-21003375 None:intergenic
ATGATATGAAAATTTATATT+TGG 0.185679 5_4:+21001596 Msa0843350:five_prime_UTR
TCTACTCCTCCTCATTATTC+AGG 0.242819 5_4:+21002095 Msa0843350:CDS
GGTATTGGATGTAACTAAAT+TGG 0.247978 5_4:+21003842 Msa0843350:CDS
GCCACCAATGTGAACCTTTA+TGG 0.250930 5_4:-21003272 None:intergenic
CCTTTATGGTGGACAGGTTT+AGG 0.257428 5_4:-21003258 None:intergenic
AGTAGAAGATGAAGTGTATA+TGG 0.261792 5_4:-21001978 None:intergenic
TGCTTAAATTGTTCCGCTTT+CGG 0.266045 5_4:+21003519 Msa0843350:CDS
GGTCTTAATTATACTGATAT+TGG 0.266549 5_4:+21003540 Msa0843350:CDS
GGAAAGGGTGAGAAGAAAAT+TGG 0.278867 5_4:+21003729 Msa0843350:CDS
TTGTGTTATCATTTGGAAAT+TGG 0.278897 5_4:+21003448 Msa0843350:CDS
CACCAACAAATGTGCCATTA+TGG 0.283857 5_4:-21004139 None:intergenic
GTGACTACTTCAATGGAAAA+TGG 0.286087 5_4:+21002977 Msa0843350:CDS
TCTCTTGTATCCTTAATTCA+TGG 0.290646 5_4:+21001265 Msa0843350:five_prime_UTR
CAAACTACCTTTACTATAGA+TGG 0.299980 5_4:+21003091 Msa0843350:CDS
GACTTGATTGTGTTATCATT+TGG 0.311025 5_4:+21003441 Msa0843350:CDS
AAGAATAAAGTACAATATTA+TGG 0.315105 5_4:-21001669 None:intergenic
TGTGTTCATTGGTGTTTGCC+TGG 0.324131 5_4:+21003951 Msa0843350:CDS
CTTGGAAGATTGCACTCATT+TGG 0.324777 5_4:-21003117 None:intergenic
AAAGAGAAATCCATGAATTA+AGG 0.326553 5_4:-21001275 None:intergenic
TGCAAAGAAGAGACTCAAAT+TGG 0.329679 5_4:-21003215 None:intergenic
TTCCATAATGGCACATTTGT+TGG 0.341005 5_4:+21004137 Msa0843350:three_prime_UTR
TGTTCATGATCCTAAGGGTT+TGG 0.341408 5_4:-21001387 None:intergenic
AGGGATTAGATTTGAGGTAT+TGG 0.342670 5_4:+21003827 Msa0843350:CDS
TGTTCAAAGACTGAACCTTA+TGG 0.369816 5_4:+21003708 Msa0843350:CDS
CCTAAACCTGTCCACCATAA+AGG 0.373037 5_4:+21003258 Msa0843350:CDS
GTTCATGATCCTAAGGGTTT+GGG 0.373466 5_4:-21001386 None:intergenic
TCTCTGATCTTCTTCCATAA+TGG 0.379762 5_4:+21004125 Msa0843350:three_prime_UTR
AGATGATGAAGAAGTGGGAT+TGG 0.380315 5_4:+21004006 Msa0843350:CDS
CACAAGGACCTAGGTTTCTT+CGG 0.389681 5_4:-21001546 None:intergenic
TCATCGCCTTGCACAAGAAA+TGG 0.392070 5_4:-21003336 None:intergenic
CCAAGATCATGTTGATTAAC+CGG 0.398828 5_4:+21001487 Msa0843350:five_prime_UTR
AAATGGAAAGCGAGATTAGA+AGG 0.400157 5_4:+21003754 Msa0843350:CDS
CCTTCAGTTTATTATGAGAA+TGG 0.403217 5_4:+21003483 Msa0843350:CDS
GGCGATGAAAGATCAAAATC+CGG 0.403584 5_4:+21003351 Msa0843350:CDS
GTGATGAGGAGGGAGGATTC+AGG 0.404932 5_4:-21001463 None:intergenic
TTCTTCTCACCCTTTCCATA+AGG 0.411285 5_4:-21003723 None:intergenic
CATGTTGTTCCATTGTATAA+AGG 0.411961 5_4:-21003018 None:intergenic
TGAAATAAAATTTAAAATAG+AGG 0.423562 5_4:-21001624 None:intergenic
TGCAAAATGATTGTGTTCAT+TGG 0.437679 5_4:+21003940 Msa0843350:CDS
CTTAGTTGGAGAAAAGTGTT+TGG 0.437733 5_4:-21003147 None:intergenic
GATGAAGTGTATATGGAAGA+AGG 0.439128 5_4:-21001971 None:intergenic
ACTTGGAGGGATTAGATTTG+AGG 0.443087 5_4:+21003821 Msa0843350:CDS
GTTGCTAAAGGGAATGGAAT+TGG 0.445312 5_4:+21003624 Msa0843350:CDS
CATCCATGTGACTACTTCAA+TGG 0.447132 5_4:+21002970 Msa0843350:CDS
TGCACACTTATCTATATTTG+AGG 0.448253 5_4:-21001711 None:intergenic
TATAAATAAAGAAGAACAAT+GGG 0.454593 5_4:-21002017 None:intergenic
GGAAGATGTTCATGATCCTA+AGG 0.456150 5_4:-21001393 None:intergenic
GACAAAATTGTATCACCAAT+GGG 0.457041 5_4:+21003058 Msa0843350:CDS
AAGGATTGAGATTGAAGAAG+TGG 0.457929 5_4:+21003773 Msa0843350:CDS
AGTTTATTATGAGAATGGTT+TGG 0.460395 5_4:+21003488 Msa0843350:CDS
CTATTCTATTCTATTCCACT+TGG 0.460694 5_4:+21001835 Msa0843350:five_prime_UTR
AAAGTGTTTGGTTCAAACCT+TGG 0.467308 5_4:-21003135 None:intergenic
TAAGCACCTGGATGACCATC+TGG 0.468486 5_4:-21003876 None:intergenic
ATGTCTCACTCTTCACTCCT+TGG 0.471169 5_4:-21004038 None:intergenic
GATGAAGAAGTGGGATTGGA+AGG 0.471664 5_4:+21004010 Msa0843350:CDS
TTGAGAGGAAAGTTGCTAAA+GGG 0.472700 5_4:+21003613 Msa0843350:CDS
ACACCGTTAGCTAACGGAAA+AGG 0.473607 5_4:-21003906 None:intergenic
ATGCAACATTATCCGTGTAT+TGG 0.475380 5_4:+21003310 Msa0843350:CDS
TCTAATTTATGATTTAATTG+AGG 0.477409 5_4:-21001517 None:intergenic
TAGTAAAGGTAGTTTGAGTC+TGG 0.478277 5_4:-21003084 None:intergenic
ATCTCATTCCAAGTATCTAT+AGG 0.478611 5_4:-21003975 None:intergenic
TTGAGACCAGATGGTCATCC+AGG 0.479603 5_4:+21003870 Msa0843350:CDS
GTGTTCATTGGTGTTTGCCT+GGG 0.480611 5_4:+21003952 Msa0843350:CDS
TGTGAACCTTTATGGTGGAC+AGG 0.481848 5_4:-21003264 None:intergenic
TGCCTGGGCCTATAGATACT+TGG 0.482132 5_4:+21003967 Msa0843350:CDS
CCGGTTAATCAACATGATCT+TGG 0.485376 5_4:-21001487 None:intergenic
AATCAAGATCCCAAACCCTT+AGG 0.488568 5_4:+21001377 Msa0843350:five_prime_UTR
ACCATAAAGGTTCACATTGG+TGG 0.496277 5_4:+21003271 Msa0843350:CDS
TCTAGAGATGATGAAGAAGT+GGG 0.498464 5_4:+21004001 Msa0843350:CDS
TTGGTTTCCATCTATAGTAA+AGG 0.502522 5_4:-21003098 None:intergenic
GTTTGAGTCTGGTCTCCCAT+TGG 0.504311 5_4:-21003073 None:intergenic
TAATAAATTAACAAGCTAAG+AGG 0.510694 5_4:-21004062 None:intergenic
GATGGTGATTGGGATAAGGC+TGG 0.512386 5_4:+21003681 Msa0843350:CDS
TTCTAGAGATGATGAAGAAG+TGG 0.512711 5_4:+21004000 Msa0843350:CDS
AAGACTGAACCTTATGGAAA+GGG 0.515408 5_4:+21003714 Msa0843350:CDS
CATAATGGCACATTTGTTGG+TGG 0.515551 5_4:+21004140 Msa0843350:three_prime_UTR
TGGTCGCCATTTCTTGTGCA+AGG 0.517981 5_4:+21003330 Msa0843350:CDS
AGGAAAGTTGCTAAAGGGAA+TGG 0.518826 5_4:+21003618 Msa0843350:CDS
TATTGTATTAAAATTTGGCC+CGG 0.524973 5_4:-21003370 None:intergenic
AGCAGAAAACAAAGTAGGAA+AGG 0.526927 5_4:+21001881 Msa0843350:five_prime_UTR
ATTGAGAGGAAAGTTGCTAA+AGG 0.529202 5_4:+21003612 Msa0843350:CDS
GGACAAAATTGTATCACCAA+TGG 0.532795 5_4:+21003057 Msa0843350:CDS
CAAATGAGTGCAATCTTCCA+AGG 0.538693 5_4:+21003118 Msa0843350:CDS
TATTCTATTCTATTCCACTT+GGG 0.540942 5_4:+21001836 Msa0843350:five_prime_UTR
GCGATGAAAGATCAAAATCC+GGG 0.544305 5_4:+21003352 Msa0843350:CDS
ATGAAGTGTATATGGAAGAA+GGG 0.544510 5_4:-21001970 None:intergenic
AAAGACTGAACCTTATGGAA+AGG 0.548583 5_4:+21003713 Msa0843350:CDS
GAAGATGTTCATGATCCTAA+GGG 0.549732 5_4:-21001392 None:intergenic
GTATAAATAAAGAAGAACAA+TGG 0.554764 5_4:-21002018 None:intergenic
ATAATGGCACATTTGTTGGT+GGG 0.555933 5_4:+21004141 Msa0843350:three_prime_UTR
TTCCAAGTATCTATAGGCCC+AGG 0.563711 5_4:-21003969 None:intergenic
ACATGTGGAACAATCAAAGA+AGG 0.566099 5_4:+21003036 Msa0843350:CDS
TTATACAATGGAACAACATG+TGG 0.569031 5_4:+21003021 Msa0843350:CDS
TGAATTAAGGATACAAGAGA+TGG 0.569147 5_4:-21001262 None:intergenic
AGTATAATTAAGACCGAAAG+CGG 0.574670 5_4:-21003532 None:intergenic
CTATGAGCAGAAAACAAAGT+AGG 0.577669 5_4:+21001876 Msa0843350:five_prime_UTR
TTGGCATTGTTGAGACCAGA+TGG 0.580975 5_4:+21003861 Msa0843350:CDS
ATGAAGAAGTGGGATTGGAA+GGG 0.584115 5_4:+21004011 Msa0843350:CDS
GAGTATAACCGAAGAAACCT+AGG 0.594607 5_4:+21001538 Msa0843350:five_prime_UTR
ATGATCTTGGTGTGATGAGG+AGG 0.601958 5_4:-21001474 None:intergenic
CAATCACCATCAAAATGAGA+AGG 0.607997 5_4:-21003669 None:intergenic
ATTAACATACCTGAATAATG+AGG 0.612033 5_4:-21002104 None:intergenic
TTCGGAACACCGTTAGCTAA+CGG 0.613273 5_4:-21003912 None:intergenic
AAGAAATGGCGACCAATACA+CGG 0.614637 5_4:-21003322 None:intergenic
TTTCCATTGAAGTAGTCACA+TGG 0.617272 5_4:-21002973 None:intergenic
CCATTCTCATAATAAACTGA+AGG 0.619413 5_4:-21003483 None:intergenic
AAAGGATTCATATAAGCACC+TGG 0.621291 5_4:-21003888 None:intergenic
ATTTAATTGAGGAAACTCAC+CGG 0.624932 5_4:-21001506 None:intergenic
GTCTTAATAGCATAATTGAG+AGG 0.633582 5_4:+21003598 Msa0843350:CDS
GGATACAAGAGATGGAGTCA+AGG 0.637728 5_4:-21001254 None:intergenic
AAAGTGTGACACAAGGACCT+AGG 0.643102 5_4:-21001555 None:intergenic
GGGATTGGAAGGGACAGCCA+AGG 0.651431 5_4:+21004021 Msa0843350:CDS
AACATGATCTTGGTGTGATG+AGG 0.658091 5_4:-21001477 None:intergenic
AACATACCTGAATAATGAGG+AGG 0.659116 5_4:-21002101 None:intergenic
AAATTGGCGAAGGATATGGA+TGG 0.663011 5_4:+21003414 Msa0843350:CDS
TCCACCATAAAGGTTCACAT+TGG 0.669651 5_4:+21003268 Msa0843350:CDS
ACCAATGTGAACCTTTATGG+TGG 0.671348 5_4:-21003269 None:intergenic
TGATCTTGGTGTGATGAGGA+GGG 0.687237 5_4:-21001473 None:intergenic
ATTGAAGAAAGTGTGACACA+AGG 0.695094 5_4:-21001562 None:intergenic
TCTTGGTGTGATGAGGAGGG+AGG 0.711294 5_4:-21001470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAATAAGATAAGAT+TGG - chr5_4:21001337-21001356 None:intergenic 10.0%
!!! AAATATAAATTTTCATATCA+TGG - chr5_4:21001597-21001616 None:intergenic 10.0%
!! ATGATATGAAAATTTATATT+TGG + chr5_4:21001596-21001615 Msa0843350:five_prime_UTR 10.0%
!!! TGAAATAAAATTTAAAATAG+AGG - chr5_4:21001627-21001646 None:intergenic 10.0%
!!! TTTTAATAAAAGATACAATA+AGG - chr5_4:21002767-21002786 None:intergenic 10.0%
!! TTAGATTTATTTAAACTATT+TGG + chr5_4:21002866-21002885 Msa0843350:intron 10.0%
!! AAATGTATTGTATTAAAATT+TGG - chr5_4:21003378-21003397 None:intergenic 10.0%
!!! AAATTTTAATACAATACATT+TGG + chr5_4:21003377-21003396 Msa0843350:CDS 10.0%
!!! TAATAAACATAATCTTTTTA+TGG - chr5_4:21004098-21004117 None:intergenic 10.0%
!! TCTAATTTATGATTTAATTG+AGG - chr5_4:21001520-21001539 None:intergenic 15.0%
!! AAGAATAAAGTACAATATTA+TGG - chr5_4:21001672-21001691 None:intergenic 15.0%
!! TATAAATAAAGAAGAACAAT+GGG - chr5_4:21002020-21002039 None:intergenic 15.0%
!!! TTTTTAAAGGAAAAATGATT+AGG + chr5_4:21002215-21002234 Msa0843350:intron 15.0%
!! GTACTATATTTAATTAAATG+AGG + chr5_4:21002327-21002346 Msa0843350:intron 15.0%
!! GTATAAATAAAGAAGAACAA+TGG - chr5_4:21002021-21002040 None:intergenic 20.0%
!!! ATGATTAGGTGTTTTTTTAA+AGG + chr5_4:21002202-21002221 Msa0843350:intron 20.0%
!! TAATTAAATGAGGTAATCAT+TGG + chr5_4:21002337-21002356 Msa0843350:intron 20.0%
!! AATATACCTTACGATAAATT+TGG - chr5_4:21002530-21002549 None:intergenic 20.0%
!! TTAAATAAATCTAATCAGAG+TGG - chr5_4:21002860-21002879 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG + chr5_4:21003563-21003582 Msa0843350:CDS 20.0%
!! TAATAAATTAACAAGCTAAG+AGG - chr5_4:21004065-21004084 None:intergenic 20.0%
! AAAGAGAAATCCATGAATTA+AGG - chr5_4:21001278-21001297 None:intergenic 25.0%
! TTGGAAGAAATGAAAAATGT+TGG - chr5_4:21001318-21001337 None:intergenic 25.0%
!!! CACAAATTTCTTGATGATTT+TGG - chr5_4:21001432-21001451 None:intergenic 25.0%
!!! TTTACTTCACGCAAATTTTT+TGG + chr5_4:21001803-21001822 Msa0843350:five_prime_UTR 25.0%
! TATTCTATTCTATTCCACTT+GGG + chr5_4:21001836-21001855 Msa0843350:five_prime_UTR 25.0%
! ATTAACATACCTGAATAATG+AGG - chr5_4:21002107-21002126 None:intergenic 25.0%
! ATTCACACTTGAAAATGATT+AGG + chr5_4:21002188-21002207 Msa0843350:intron 25.0%
! TGTTGAATTGAACACTAAAA+TGG + chr5_4:21002240-21002259 Msa0843350:intron 25.0%
! TTCAACTACTCTAATTAACA+TGG - chr5_4:21002285-21002304 None:intergenic 25.0%
! TTAAACACTTATAGTGAGAA+AGG + chr5_4:21002416-21002435 Msa0843350:intron 25.0%
!! TATCGTAAGGTATATTTCTA+TGG + chr5_4:21002534-21002553 Msa0843350:intron 25.0%
! TCGAAAAATATATGTTTCGA+CGG + chr5_4:21002592-21002611 Msa0843350:intron 25.0%
!! AAATGGATTCATGACAAAAA+AGG + chr5_4:21002994-21003013 Msa0843350:CDS 25.0%
! AAAAAAGGTCCTTTATACAA+TGG + chr5_4:21003009-21003028 Msa0843350:CDS 25.0%
!! AACAAACACATATCTTTTGT+TGG + chr5_4:21003174-21003193 Msa0843350:CDS 25.0%
! TATTGTATTAAAATTTGGCC+CGG - chr5_4:21003373-21003392 None:intergenic 25.0%
! TTGTGTTATCATTTGGAAAT+TGG + chr5_4:21003448-21003467 Msa0843350:CDS 25.0%
! AGTTTATTATGAGAATGGTT+TGG + chr5_4:21003488-21003507 Msa0843350:CDS 25.0%
! GGTCTTAATTATACTGATAT+TGG + chr5_4:21003540-21003559 Msa0843350:CDS 25.0%
! TGAGAAGAAAATTGGAAAAA+TGG + chr5_4:21003737-21003756 Msa0843350:CDS 25.0%
! AAGAAAAAAGCAAATGAACT+TGG + chr5_4:21003804-21003823 Msa0843350:CDS 25.0%
TGAATTAAGGATACAAGAGA+TGG - chr5_4:21001265-21001284 None:intergenic 30.0%
TCTCTTGTATCCTTAATTCA+TGG + chr5_4:21001265-21001284 Msa0843350:five_prime_UTR 30.0%
! AATGTTGGCAAGTTTTACAA+AGG - chr5_4:21001303-21001322 None:intergenic 30.0%
!! TTCTTGATGATTTTGGAGAA+AGG - chr5_4:21001425-21001444 None:intergenic 30.0%
ATTTAATTGAGGAAACTCAC+CGG - chr5_4:21001509-21001528 None:intergenic 30.0%
TGCACACTTATCTATATTTG+AGG - chr5_4:21001714-21001733 None:intergenic 30.0%
CTATTCTATTCTATTCCACT+TGG + chr5_4:21001835-21001854 Msa0843350:five_prime_UTR 30.0%
ATGAAGTGTATATGGAAGAA+GGG - chr5_4:21001973-21001992 None:intergenic 30.0%
AGTAGAAGATGAAGTGTATA+TGG - chr5_4:21001981-21002000 None:intergenic 30.0%
! GAGATTTTGTAAGAGACAAA+GGG - chr5_4:21002049-21002068 None:intergenic 30.0%
! TGAGATTTTGTAAGAGACAA+AGG - chr5_4:21002050-21002069 None:intergenic 30.0%
TACCTTACGATAAATTTGGA+GGG - chr5_4:21002526-21002545 None:intergenic 30.0%
ATACCTTACGATAAATTTGG+AGG - chr5_4:21002527-21002546 None:intergenic 30.0%
TTAAAAGTGAGAAGAGCTTT+CGG - chr5_4:21002735-21002754 None:intergenic 30.0%
AAACCTCTGATATACAATCA+TGG + chr5_4:21002817-21002836 Msa0843350:intron 30.0%
AACCTCTGATATACAATCAT+GGG + chr5_4:21002818-21002837 Msa0843350:intron 30.0%
CATGTTGTTCCATTGTATAA+AGG - chr5_4:21003021-21003040 None:intergenic 30.0%
TTATACAATGGAACAACATG+TGG + chr5_4:21003021-21003040 Msa0843350:CDS 30.0%
GACAAAATTGTATCACCAAT+GGG + chr5_4:21003058-21003077 Msa0843350:CDS 30.0%
CAAACTACCTTTACTATAGA+TGG + chr5_4:21003091-21003110 Msa0843350:CDS 30.0%
TTGGTTTCCATCTATAGTAA+AGG - chr5_4:21003101-21003120 None:intergenic 30.0%
!!! TGTGTTTGTTTTTGCTTAGT+TGG - chr5_4:21003164-21003183 None:intergenic 30.0%
GACTTGATTGTGTTATCATT+TGG + chr5_4:21003441-21003460 Msa0843350:CDS 30.0%
CCATTCTCATAATAAACTGA+AGG - chr5_4:21003486-21003505 None:intergenic 30.0%
CCTTCAGTTTATTATGAGAA+TGG + chr5_4:21003483-21003502 Msa0843350:CDS 30.0%
!!! TATGAGAATGGTTTGGTTTT+AGG + chr5_4:21003495-21003514 Msa0843350:CDS 30.0%
AGTATAATTAAGACCGAAAG+CGG - chr5_4:21003535-21003554 None:intergenic 30.0%
TTCCATTAAGAAAGGCTTTA+AGG + chr5_4:21003571-21003590 Msa0843350:CDS 30.0%
GTCTTAATAGCATAATTGAG+AGG + chr5_4:21003598-21003617 Msa0843350:CDS 30.0%
! TTTTCACCTTCTCATTTTGA+TGG + chr5_4:21003663-21003682 Msa0843350:CDS 30.0%
!!! TTCTCATTTTGATGGTGATT+GGG + chr5_4:21003671-21003690 Msa0843350:CDS 30.0%
AAAAAAGCAAATGAACTTGG+AGG + chr5_4:21003807-21003826 Msa0843350:CDS 30.0%
AAAAAGCAAATGAACTTGGA+GGG + chr5_4:21003808-21003827 Msa0843350:CDS 30.0%
! GGTATTGGATGTAACTAAAT+TGG + chr5_4:21003842-21003861 Msa0843350:CDS 30.0%
! CAATCATTTTGCACATACTT+CGG - chr5_4:21003933-21003952 None:intergenic 30.0%
TGCAAAATGATTGTGTTCAT+TGG + chr5_4:21003940-21003959 Msa0843350:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG - chr5_4:21003978-21003997 None:intergenic 30.0%
GAAGATGTTCATGATCCTAA+GGG - chr5_4:21001395-21001414 None:intergenic 35.0%
!! GATTTTGGAGAAAGGAGTTT+TGG - chr5_4:21001417-21001436 None:intergenic 35.0%
! CCAAGATCATGTTGATTAAC+CGG + chr5_4:21001487-21001506 Msa0843350:five_prime_UTR 35.0%
! ATTGAAGAAAGTGTGACACA+AGG - chr5_4:21001565-21001584 None:intergenic 35.0%
CTATGAGCAGAAAACAAAGT+AGG + chr5_4:21001876-21001895 Msa0843350:five_prime_UTR 35.0%
AGCAGAAAACAAAGTAGGAA+AGG + chr5_4:21001881-21001900 Msa0843350:five_prime_UTR 35.0%
GATGAAGTGTATATGGAAGA+AGG - chr5_4:21001974-21001993 None:intergenic 35.0%
AACATACCTGAATAATGAGG+AGG - chr5_4:21002104-21002123 None:intergenic 35.0%
! TAGTGAGAAAGGTACAAGTA+AGG + chr5_4:21002427-21002446 Msa0843350:intron 35.0%
TACCTAAACTGGATATCATG+AGG - chr5_4:21002470-21002489 None:intergenic 35.0%
TAAAGAGCAACTACCTAAAC+TGG - chr5_4:21002481-21002500 None:intergenic 35.0%
! TTCCTGTTTAAAGCAACACA+TGG + chr5_4:21002622-21002641 Msa0843350:intron 35.0%
!! TTCCATGTGTTGCTTTAAAC+AGG - chr5_4:21002627-21002646 None:intergenic 35.0%
!! TTGTCACCGTTTTGATGTTT+TGG + chr5_4:21002671-21002690 Msa0843350:intron 35.0%
!! TGTCACCGTTTTGATGTTTT+GGG + chr5_4:21002672-21002691 Msa0843350:intron 35.0%
TTTCCATTGAAGTAGTCACA+TGG - chr5_4:21002976-21002995 None:intergenic 35.0%
GTGACTACTTCAATGGAAAA+TGG + chr5_4:21002977-21002996 Msa0843350:CDS 35.0%
ACATGTGGAACAATCAAAGA+AGG + chr5_4:21003036-21003055 Msa0843350:CDS 35.0%
GGACAAAATTGTATCACCAA+TGG + chr5_4:21003057-21003076 Msa0843350:CDS 35.0%
TAGTAAAGGTAGTTTGAGTC+TGG - chr5_4:21003087-21003106 None:intergenic 35.0%
! AAAGTGTTTGGTTCAAACCT+TGG - chr5_4:21003138-21003157 None:intergenic 35.0%
!! CTTAGTTGGAGAAAAGTGTT+TGG - chr5_4:21003150-21003169 None:intergenic 35.0%
TGCAAAGAAGAGACTCAAAT+TGG - chr5_4:21003218-21003237 None:intergenic 35.0%
ATGCAACATTATCCGTGTAT+TGG + chr5_4:21003310-21003329 Msa0843350:CDS 35.0%
GGATCGCGTTAATGAAAAAT+TGG + chr5_4:21003398-21003417 Msa0843350:CDS 35.0%
CGTTAATGAAAAATTGGCGA+AGG + chr5_4:21003404-21003423 Msa0843350:CDS 35.0%
TGAAAAATTGGCGAAGGATA+TGG + chr5_4:21003410-21003429 Msa0843350:CDS 35.0%
TGCTTAAATTGTTCCGCTTT+CGG + chr5_4:21003519-21003538 Msa0843350:CDS 35.0%
GTCCTTAAAGCCTTTCTTAA+TGG - chr5_4:21003576-21003595 None:intergenic 35.0%
ATTGAGAGGAAAGTTGCTAA+AGG + chr5_4:21003612-21003631 Msa0843350:CDS 35.0%
TTGAGAGGAAAGTTGCTAAA+GGG + chr5_4:21003613-21003632 Msa0843350:CDS 35.0%
CAATCACCATCAAAATGAGA+AGG - chr5_4:21003672-21003691 None:intergenic 35.0%
!!! CTTCTCATTTTGATGGTGAT+TGG + chr5_4:21003670-21003689 Msa0843350:CDS 35.0%
!!! TTTTGATGGTGATTGGGATA+AGG + chr5_4:21003677-21003696 Msa0843350:CDS 35.0%
TGTTCAAAGACTGAACCTTA+TGG + chr5_4:21003708-21003727 Msa0843350:CDS 35.0%
AAAGACTGAACCTTATGGAA+AGG + chr5_4:21003713-21003732 Msa0843350:CDS 35.0%
AAGACTGAACCTTATGGAAA+GGG + chr5_4:21003714-21003733 Msa0843350:CDS 35.0%
AAATGGAAAGCGAGATTAGA+AGG + chr5_4:21003754-21003773 Msa0843350:CDS 35.0%
AAGGATTGAGATTGAAGAAG+TGG + chr5_4:21003773-21003792 Msa0843350:CDS 35.0%
! AGGGATTAGATTTGAGGTAT+TGG + chr5_4:21003827-21003846 Msa0843350:CDS 35.0%
AAAGGATTCATATAAGCACC+TGG - chr5_4:21003891-21003910 None:intergenic 35.0%
! AATCCTTTTCCGTTAGCTAA+CGG + chr5_4:21003903-21003922 Msa0843350:CDS 35.0%
! TTCTAGAGATGATGAAGAAG+TGG + chr5_4:21004000-21004019 Msa0843350:CDS 35.0%
TCTAGAGATGATGAAGAAGT+GGG + chr5_4:21004001-21004020 Msa0843350:CDS 35.0%
TCTCTGATCTTCTTCCATAA+TGG + chr5_4:21004125-21004144 Msa0843350:three_prime_UTR 35.0%
TTCCATAATGGCACATTTGT+TGG + chr5_4:21004137-21004156 Msa0843350:three_prime_UTR 35.0%
! ATAATGGCACATTTGTTGGT+GGG + chr5_4:21004141-21004160 Msa0843350:three_prime_UTR 35.0%
AATCAAGATCCCAAACCCTT+AGG + chr5_4:21001377-21001396 Msa0843350:five_prime_UTR 40.0%
GTTCATGATCCTAAGGGTTT+GGG - chr5_4:21001389-21001408 None:intergenic 40.0%
TGTTCATGATCCTAAGGGTT+TGG - chr5_4:21001390-21001409 None:intergenic 40.0%
GGAAGATGTTCATGATCCTA+AGG - chr5_4:21001396-21001415 None:intergenic 40.0%
! AACATGATCTTGGTGTGATG+AGG - chr5_4:21001480-21001499 None:intergenic 40.0%
CCGGTTAATCAACATGATCT+TGG - chr5_4:21001490-21001509 None:intergenic 40.0%
GAGTATAACCGAAGAAACCT+AGG + chr5_4:21001538-21001557 Msa0843350:five_prime_UTR 40.0%
TCTACTCCTCCTCATTATTC+AGG + chr5_4:21002095-21002114 Msa0843350:CDS 40.0%
! AAAGGTACAAGTAAGGCCTT+TGG + chr5_4:21002434-21002453 Msa0843350:intron 40.0%
CACCTCATGATATCCAGTTT+AGG + chr5_4:21002465-21002484 Msa0843350:intron 40.0%
AGCCCTCCAAATTTATCGTA+AGG + chr5_4:21002521-21002540 Msa0843350:intron 40.0%
TCTGTAGATGGTTGCTCTTT+AGG - chr5_4:21002650-21002669 None:intergenic 40.0%
AACGGTGACAATTCTGTAGA+TGG - chr5_4:21002662-21002681 None:intergenic 40.0%
CACCCATGATTGTATATCAG+AGG - chr5_4:21002823-21002842 None:intergenic 40.0%
CATCCATGTGACTACTTCAA+TGG + chr5_4:21002970-21002989 Msa0843350:CDS 40.0%
CTTGGAAGATTGCACTCATT+TGG - chr5_4:21003120-21003139 None:intergenic 40.0%
CAAATGAGTGCAATCTTCCA+AGG + chr5_4:21003118-21003137 Msa0843350:CDS 40.0%
TCCACCATAAAGGTTCACAT+TGG + chr5_4:21003268-21003287 Msa0843350:CDS 40.0%
ACCAATGTGAACCTTTATGG+TGG - chr5_4:21003272-21003291 None:intergenic 40.0%
ACCATAAAGGTTCACATTGG+TGG + chr5_4:21003271-21003290 Msa0843350:CDS 40.0%
AAGAAATGGCGACCAATACA+CGG - chr5_4:21003325-21003344 None:intergenic 40.0%
GGCGATGAAAGATCAAAATC+CGG + chr5_4:21003351-21003370 Msa0843350:CDS 40.0%
GCGATGAAAGATCAAAATCC+GGG + chr5_4:21003352-21003371 Msa0843350:CDS 40.0%
AAATTGGCGAAGGATATGGA+TGG + chr5_4:21003414-21003433 Msa0843350:CDS 40.0%
AGGAAAGTTGCTAAAGGGAA+TGG + chr5_4:21003618-21003637 Msa0843350:CDS 40.0%
GTTGCTAAAGGGAATGGAAT+TGG + chr5_4:21003624-21003643 Msa0843350:CDS 40.0%
TTCTTCTCACCCTTTCCATA+AGG - chr5_4:21003726-21003745 None:intergenic 40.0%
GGAAAGGGTGAGAAGAAAAT+TGG + chr5_4:21003729-21003748 Msa0843350:CDS 40.0%
ACTTGGAGGGATTAGATTTG+AGG + chr5_4:21003821-21003840 Msa0843350:CDS 40.0%
AGATGATGAAGAAGTGGGAT+TGG + chr5_4:21004006-21004025 Msa0843350:CDS 40.0%
ATGAAGAAGTGGGATTGGAA+GGG + chr5_4:21004011-21004030 Msa0843350:CDS 40.0%
! CACCAACAAATGTGCCATTA+TGG - chr5_4:21004142-21004161 None:intergenic 40.0%
! CATAATGGCACATTTGTTGG+TGG + chr5_4:21004140-21004159 Msa0843350:three_prime_UTR 40.0%
!!! TGGTGGGAGACTTTTGTTTT+TGG + chr5_4:21004157-21004176 Msa0843350:three_prime_UTR 40.0%
GGATACAAGAGATGGAGTCA+AGG - chr5_4:21001257-21001276 None:intergenic 45.0%
! TGATCTTGGTGTGATGAGGA+GGG - chr5_4:21001476-21001495 None:intergenic 45.0%
! ATGATCTTGGTGTGATGAGG+AGG - chr5_4:21001477-21001496 None:intergenic 45.0%
CACAAGGACCTAGGTTTCTT+CGG - chr5_4:21001549-21001568 None:intergenic 45.0%
AAAGTGTGACACAAGGACCT+AGG - chr5_4:21001558-21001577 None:intergenic 45.0%
! CTCTTTTGTGATGTCCCAAG+TGG - chr5_4:21001853-21001872 None:intergenic 45.0%
! ATGAGGTGTCATGAGACCAA+AGG - chr5_4:21002453-21002472 None:intergenic 45.0%
TGGAGGGCTTCATAGCAATA+CGG - chr5_4:21002510-21002529 None:intergenic 45.0%
!! ACCGTTTTGATGTTTTGGGC+CGG + chr5_4:21002676-21002695 Msa0843350:intron 45.0%
ACCGGCCCAAAACATCAAAA+CGG - chr5_4:21002680-21002699 None:intergenic 45.0%
!! TGATGTTTTGGGCCGGTTAA+TGG + chr5_4:21002683-21002702 Msa0843350:intron 45.0%
!! GATGTTTTGGGCCGGTTAAT+GGG + chr5_4:21002684-21002703 Msa0843350:intron 45.0%
TCGGTGTAACTAAACTCCGA+GGG - chr5_4:21002716-21002735 None:intergenic 45.0%
! TTCGGTGTAACTAAACTCCG+AGG - chr5_4:21002717-21002736 None:intergenic 45.0%
!! CCTTTATGGTGGACAGGTTT+AGG - chr5_4:21003261-21003280 None:intergenic 45.0%
CCTAAACCTGTCCACCATAA+AGG + chr5_4:21003258-21003277 Msa0843350:CDS 45.0%
!! TGTGAACCTTTATGGTGGAC+AGG - chr5_4:21003267-21003286 None:intergenic 45.0%
GCCACCAATGTGAACCTTTA+TGG - chr5_4:21003275-21003294 None:intergenic 45.0%
TCATCGCCTTGCACAAGAAA+TGG - chr5_4:21003339-21003358 None:intergenic 45.0%
TTGGCATTGTTGAGACCAGA+TGG + chr5_4:21003861-21003880 Msa0843350:CDS 45.0%
ACACCGTTAGCTAACGGAAA+AGG - chr5_4:21003909-21003928 None:intergenic 45.0%
TTCGGAACACCGTTAGCTAA+CGG - chr5_4:21003915-21003934 None:intergenic 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG + chr5_4:21003951-21003970 Msa0843350:CDS 45.0%
! GTGTTCATTGGTGTTTGCCT+GGG + chr5_4:21003952-21003971 Msa0843350:CDS 45.0%
TTCCAAGTATCTATAGGCCC+AGG - chr5_4:21003972-21003991 None:intergenic 45.0%
GATGAAGAAGTGGGATTGGA+AGG + chr5_4:21004010-21004029 Msa0843350:CDS 45.0%
ATGTCTCACTCTTCACTCCT+TGG - chr5_4:21004041-21004060 None:intergenic 45.0%
GGGCTTCATAGCAATACGGA+GGG - chr5_4:21002506-21002525 None:intergenic 50.0%
AGGGCTTCATAGCAATACGG+AGG - chr5_4:21002507-21002526 None:intergenic 50.0%
GTTTGAGTCTGGTCTCCCAT+TGG - chr5_4:21003076-21003095 None:intergenic 50.0%
TGGTCGCCATTTCTTGTGCA+AGG + chr5_4:21003330-21003349 Msa0843350:CDS 50.0%
GATGGTGATTGGGATAAGGC+TGG + chr5_4:21003681-21003700 Msa0843350:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG + chr5_4:21003870-21003889 Msa0843350:CDS 50.0%
TAAGCACCTGGATGACCATC+TGG - chr5_4:21003879-21003898 None:intergenic 50.0%
TGCCTGGGCCTATAGATACT+TGG + chr5_4:21003967-21003986 Msa0843350:CDS 50.0%
GTGATGAGGAGGGAGGATTC+AGG - chr5_4:21001466-21001485 None:intergenic 55.0%
! TCTTGGTGTGATGAGGAGGG+AGG - chr5_4:21001473-21001492 None:intergenic 55.0%
TAACTAAACTCCGAGGGCCG+TGG - chr5_4:21002710-21002729 None:intergenic 55.0%
! TTGGGCCGGTTAATGGGCCA+CGG + chr5_4:21002690-21002709 Msa0843350:intron 60.0%
GGGATTGGAAGGGACAGCCA+AGG + chr5_4:21004021-21004040 Msa0843350:CDS 60.0%
GAGGGCCGTGGCCCATTAAC+CGG - chr5_4:21002698-21002717 None:intergenic 65.0%
! GGTTAATGGGCCACGGCCCT+CGG + chr5_4:21002697-21002716 Msa0843350:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 21001249 21004180 21001249 ID=Msa0843350;Name=Msa0843350
chr5_4 mRNA 21001249 21004180 21001249 ID=Msa0843350-mRNA-1;Parent=Msa0843350;Name=Msa0843350-mRNA-1;_AED=0.05;_eAED=0.05;_QI=678|1|1|1|0.5|0.33|3|93|433
chr5_4 exon 21001249 21002116 21001249 ID=Msa0843350-mRNA-1:exon:8693;Parent=Msa0843350-mRNA-1
chr5_4 exon 21002944 21004072 21002944 ID=Msa0843350-mRNA-1:exon:8694;Parent=Msa0843350-mRNA-1
chr5_4 exon 21004105 21004180 21004105 ID=Msa0843350-mRNA-1:exon:8695;Parent=Msa0843350-mRNA-1
chr5_4 five_prime_UTR 21001249 21001926 21001249 ID=Msa0843350-mRNA-1:five_prime_utr;Parent=Msa0843350-mRNA-1
chr5_4 CDS 21001927 21002116 21001927 ID=Msa0843350-mRNA-1:cds;Parent=Msa0843350-mRNA-1
chr5_4 CDS 21002944 21004055 21002944 ID=Msa0843350-mRNA-1:cds;Parent=Msa0843350-mRNA-1
chr5_4 three_prime_UTR 21004056 21004072 21004056 ID=Msa0843350-mRNA-1:three_prime_utr;Parent=Msa0843350-mRNA-1
chr5_4 three_prime_UTR 21004105 21004180 21004105 ID=Msa0843350-mRNA-1:three_prime_utr;Parent=Msa0843350-mRNA-1
Gene Sequence

>Msa0843350

ATGAAAAGTACAATGAAAGATGATTCAATTTCACTCTCTAAGACCCTTCTTCCATATACACTTCATCTTCTACTTCTCATAGCTCTCTTACCCATTGTTCTTCTTTATTTATACTTTTACCCTTTGTCTCTTACAAAATCTCAAACTCAGCTTCATCATGCAACTATTTCTACTCCTCCTCATTATTCAGTAAATGACAAACACAAAATTGATGAGCATCCATGTGACTACTTCAATGGAAAATGGATTCATGACAAAAAAGGTCCTTTATACAATGGAACAACATGTGGAACAATCAAAGAAGGACAAAATTGTATCACCAATGGGAGACCAGACTCAAACTACCTTTACTATAGATGGAAACCAAATGAGTGCAATCTTCCAAGGTTTGAACCAAACACTTTTCTCCAACTAAGCAAAAACAAACACATATCTTTTGTTGGTGATTCTTTAGCTAGAAACCAATTTGAGTCTCTTCTTTGCATGTTATCAATTGTTTCAAAACCTAAACCTGTCCACCATAAAGGTTCACATTGGTGGCATTTTTCTTCTCATAATGCAACATTATCCGTGTATTGGTCGCCATTTCTTGTGCAAGGCGATGAAAGATCAAAATCCGGGCCAAATTTTAATACAATACATTTGGATCGCGTTAATGAAAAATTGGCGAAGGATATGGATGGAATCGACTTGATTGTGTTATCATTTGGAAATTGGTTTATTAATGTTCCTTCAGTTTATTATGAGAATGGTTTGGTTTTAGGATGCTTAAATTGTTCCGCTTTCGGTCTTAATTATACTGATATTGGTTTTTATGTTCCATTAAGAAAGGCTTTAAGGACTTGTCTTAATAGCATAATTGAGAGGAAAGTTGCTAAAGGGAATGGAATTGGTGTAATTGTGAAAACTTTTTCACCTTCTCATTTTGATGGTGATTGGGATAAGGCTGGAACATGTTCAAAGACTGAACCTTATGGAAAGGGTGAGAAGAAAATTGGAAAAATGGAAAGCGAGATTAGAAGGATTGAGATTGAAGAAGTGGAAAATGCTAAGAAAAAAGCAAATGAACTTGGAGGGATTAGATTTGAGGTATTGGATGTAACTAAATTGGCATTGTTGAGACCAGATGGTCATCCAGGTGCTTATATGAATCCTTTTCCGTTAGCTAACGGTGTTCCGAAGTATGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGGCCTATAGATACTTGGAATGAGATTTTTCTAGAGATGATGAAGAAGTGGGATTGGAAGGGACAGCCAAGGAGTGAAGAGTGA
Protein sequence

>Msa0843350

MKSTMKDDSISLSKTLLPYTLHLLLLIALLPIVLLYLYFYPLSLTKSQTQLHHATISTPPHYSVNDKHKIDEHPCDYFNGKWIHDKKGPLYNGTTCGTIKEGQNCITNGRPDSNYLYYRWKPNECNLPRFEPNTFLQLSKNKHISFVGDSLARNQFESLLCMLSIVSKPKPVHHKGSHWWHFSSHNATLSVYWSPFLVQGDERSKSGPNFNTIHLDRVNEKLAKDMDGIDLIVLSFGNWFINVPSVYYENGLVLGCLNCSAFGLNYTDIGFYVPLRKALRTCLNSIIERKVAKGNGIGVIVKTFSPSHFDGDWDKAGTCSKTEPYGKGEKKIGKMESEIRRIEIEEVENAKKKANELGGIRFEVLDVTKLALLRPDGHPGAYMNPFPLANGVPKYVQNDCVHWCLPGPIDTWNEIFLEMMKKWDWKGQPRSEE*