AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0806210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806210 MtrunA17_Chr5g0414051 96.074 433 16 1 1 432 1 433 0.0 822
Msa0806210 MtrunA17_Chr5g0414091 66.129 434 123 7 7 423 9 435 0.0 552
Msa0806210 MtrunA17_Chr8g0391481 67.935 368 113 3 57 420 7 373 0.0 535
Msa0806210 MtrunA17_Chr5g0414061 61.860 430 146 7 5 423 20 442 0.0 521
Msa0806210 MtrunA17_Chr5g0414041 61.765 442 147 6 5 432 11 444 0.0 518
Msa0806210 MtrunA17_Chr3g0140371 68.908 357 108 3 65 421 9 362 0.0 516
Msa0806210 MtrunA17_Chr7g0226991 66.106 357 119 2 66 421 49 404 4.14e-179 506
Msa0806210 MtrunA17_Chr5g0414081 72.500 200 54 1 222 421 3 201 1.60e-108 318
Msa0806210 MtrunA17_Chr8g0387921 39.372 414 230 11 19 419 14 419 6.46e-97 296
Msa0806210 MtrunA17_Chr8g0347321 38.776 392 220 9 40 419 56 439 8.68e-95 292
Msa0806210 MtrunA17_Chr6g0456691 37.381 420 235 12 14 420 16 420 2.11e-93 288
Msa0806210 MtrunA17_Chr6g0456681 38.272 405 222 12 27 420 22 409 5.22e-86 269
Msa0806210 MtrunA17_Chr6g0451321 34.086 443 242 13 17 421 29 459 6.07e-81 257
Msa0806210 MtrunA17_Chr2g0283851 37.119 361 195 14 77 421 58 402 1.55e-75 241
Msa0806210 MtrunA17_Chr1g0188191 34.722 360 213 9 74 421 234 583 2.34e-67 225
Msa0806210 MtrunA17_Chr6g0476041 34.072 361 214 10 74 421 167 516 3.21e-64 215
Msa0806210 MtrunA17_Chr7g0259711 33.244 373 195 17 74 421 91 434 2.75e-52 181
Msa0806210 MtrunA17_Chr1g0175671 34.060 367 199 14 74 421 170 512 6.68e-51 179
Msa0806210 MtrunA17_Chr2g0287901 32.782 363 213 10 74 423 97 441 2.27e-49 174
Msa0806210 MtrunA17_Chr7g0258851 33.333 378 207 14 74 430 248 601 1.71e-48 174
Msa0806210 MtrunA17_Chr7g0221281 36.437 247 140 9 179 420 3 237 2.91e-47 163
Msa0806210 MtrunA17_Chr8g0384151 31.944 360 198 14 74 419 90 416 1.22e-45 163
Msa0806210 MtrunA17_Chr4g0071391 31.680 363 206 13 74 419 109 446 6.77e-44 159
Msa0806210 MtrunA17_Chr4g0006991 30.890 382 210 16 63 419 75 427 1.15e-43 158
Msa0806210 MtrunA17_Chr1g0213281 30.521 403 220 18 59 430 155 528 2.36e-43 159
Msa0806210 MtrunA17_Chr3g0105301 32.022 356 210 10 70 415 39 372 2.41e-43 156
Msa0806210 MtrunA17_Chr5g0430371 30.028 363 224 10 66 415 50 395 4.83e-42 153
Msa0806210 MtrunA17_Chr3g0126021 29.384 422 236 17 26 419 72 459 6.60e-41 151
Msa0806210 MtrunA17_Chr7g0269991 30.362 359 203 13 74 416 137 464 1.53e-40 150
Msa0806210 MtrunA17_Chr8g0368461 29.306 389 209 16 74 425 178 537 2.15e-40 151
Msa0806210 MtrunA17_Chr4g0070341 27.793 367 217 9 74 419 132 471 2.99e-40 149
Msa0806210 MtrunA17_Chr8g0360521 29.144 374 212 13 71 421 68 411 1.16e-39 147
Msa0806210 MtrunA17_Chr5g0413271 29.670 364 221 14 68 421 140 478 1.43e-39 148
Msa0806210 MtrunA17_Chr1g0171171 26.750 400 252 9 29 419 8 375 1.83e-39 145
Msa0806210 MtrunA17_Chr4g0068111 28.333 420 248 13 21 419 66 453 1.87e-39 147
Msa0806210 MtrunA17_Chr3g0113671 28.809 361 223 13 71 415 103 445 5.71e-39 145
Msa0806210 MtrunA17_Chr8g0384421 28.191 376 204 14 74 419 133 472 8.95e-39 145
Msa0806210 MtrunA17_Chr7g0242701 29.427 384 213 17 63 419 75 427 1.56e-38 144
Msa0806210 MtrunA17_Chr2g0325481 31.818 352 189 15 74 414 50 361 9.95e-38 140
Msa0806210 MtrunA17_Chr7g0221271 35.751 193 112 4 14 197 16 205 1.18e-37 135
Msa0806210 MtrunA17_Chr3g0097891 30.571 350 201 11 74 415 31 346 1.53e-36 137
Msa0806210 MtrunA17_Chr3g0085081 30.484 351 198 15 74 415 31 344 4.85e-36 135
Msa0806210 MtrunA17_Chr3g0091031 30.056 356 193 16 74 415 31 344 2.04e-35 134
Msa0806210 MtrunA17_Chr3g0085071 30.769 351 197 15 74 415 46 359 2.17e-35 134
Msa0806210 MtrunA17_Chr4g0005491 27.546 432 267 18 1 415 1 403 5.04e-35 134
Msa0806210 MtrunA17_Chr4g0041011 29.310 406 231 17 24 419 4 363 6.15e-34 130
Msa0806210 MtrunA17_Chr7g0243301 27.053 414 245 18 26 415 25 405 1.28e-33 130
Msa0806210 MtrunA17_Chr6g0455901 26.941 438 244 18 22 419 15 416 2.50e-33 129
Msa0806210 MtrunA17_Chr4g0045951 28.462 390 227 12 33 412 8 355 3.29e-33 128
Msa0806210 MtrunA17_Chr2g0314801 30.056 356 210 14 71 419 30 353 5.13e-33 127
Msa0806210 MtrunA17_Chr3g0114101 27.446 368 213 15 74 416 89 427 1.45e-32 127
Msa0806210 MtrunA17_Chr5g0437601 26.431 367 224 14 71 416 80 421 5.30e-32 126
Msa0806210 MtrunA17_Chr5g0413851 30.168 358 206 12 66 415 34 355 7.20e-32 124
Msa0806210 MtrunA17_Chr4g0045941 25.428 409 226 12 17 412 1 343 8.30e-32 124
Msa0806210 MtrunA17_Chr2g0314791 28.767 365 199 15 71 419 26 345 1.85e-31 123
Msa0806210 MtrunA17_Chr2g0283581 28.109 402 231 17 28 419 5 358 5.76e-31 122
Msa0806210 MtrunA17_Chr7g0221291 36.478 159 92 3 13 165 15 170 1.09e-30 116
Msa0806210 MtrunA17_Chr4g0005481 28.000 375 217 16 68 419 102 446 4.61e-30 121
Msa0806210 MtrunA17_Chr5g0437291 27.297 370 214 17 74 415 165 507 1.84e-29 120
Msa0806210 MtrunA17_Chr4g0049421 26.596 376 215 13 74 419 48 392 4.73e-26 108
Msa0806210 MtrunA17_Chr1g0210771 33.728 169 104 4 74 239 59 222 1.28e-25 107
Msa0806210 MtrunA17_Chr1g0178081 31.395 258 150 11 17 258 5 251 8.83e-24 102
Msa0806210 MtrunA17_Chr1g0178081 34.783 115 61 5 309 421 242 344 4.50e-12 67.0
Msa0806210 MtrunA17_Chr1g0209921 45.918 98 51 2 67 162 37 134 1.07e-21 94.4
Msa0806210 MtrunA17_Chr7g0275501 54.098 61 25 1 358 418 11 68 4.06e-16 72.8
Msa0806210 MtrunA17_Chr4g0057171 40.217 92 46 3 212 303 1 83 7.75e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806210 AT1G70230.1 47.837 416 183 10 23 422 17 414 1.57e-134 393
Msa0806210 AT1G01430.1 40.369 379 204 11 53 419 79 447 6.16e-94 290
Msa0806210 AT4G23790.1 38.425 419 229 12 24 419 15 427 7.90e-94 289
Msa0806210 AT4G01080.1 39.386 391 221 8 32 417 49 428 9.69e-94 290
Msa0806210 AT4G11090.1 42.382 361 189 10 69 419 74 425 4.44e-90 280
Msa0806210 AT5G15890.1 38.287 397 218 11 31 420 147 523 9.38e-90 282
Msa0806210 AT3G02440.1 38.827 358 203 8 74 425 127 474 1.57e-86 272
Msa0806210 AT3G02440.2 38.827 358 203 8 74 425 135 482 1.85e-86 272
Msa0806210 AT3G28150.1 36.605 377 211 10 59 421 50 412 3.04e-84 264
Msa0806210 AT5G15900.1 36.740 362 208 11 74 426 67 416 1.87e-80 255
Msa0806210 AT5G15900.2 36.740 362 208 11 74 426 86 435 2.35e-80 255
Msa0806210 AT4G25360.1 33.880 366 216 9 71 421 172 526 4.10e-64 215
Msa0806210 AT4G25360.2 33.880 366 216 9 71 421 172 526 4.10e-64 215
Msa0806210 AT5G51640.1 33.067 375 225 12 71 430 135 498 2.63e-61 207
Msa0806210 AT5G19160.1 32.620 374 218 9 74 430 99 455 4.00e-54 187
Msa0806210 AT5G06700.1 32.791 369 201 15 74 421 255 597 4.30e-48 174
Msa0806210 AT3G11570.1 30.476 420 240 15 22 419 30 419 1.76e-47 168
Msa0806210 AT3G06080.2 30.769 364 217 10 74 421 103 447 2.76e-47 169
Msa0806210 AT3G12060.1 31.830 377 210 15 74 429 196 546 9.55e-47 169
Msa0806210 AT2G30010.1 29.923 391 246 10 40 419 23 396 3.55e-46 164
Msa0806210 AT1G48880.1 34.254 362 189 15 69 416 110 436 3.88e-46 165
Msa0806210 AT5G64020.1 30.027 373 213 12 74 419 57 408 7.78e-46 164
Msa0806210 AT2G30010.2 30.711 394 239 13 40 419 66 439 9.76e-46 164
Msa0806210 AT5G06230.2 31.806 371 201 13 74 419 21 364 1.10e-42 154
Msa0806210 AT5G06230.1 31.806 371 201 13 74 419 62 405 2.22e-42 154
Msa0806210 AT5G20590.1 32.222 360 201 14 74 415 141 475 3.27e-42 155
Msa0806210 AT5G58600.1 29.972 357 217 10 74 419 66 400 7.31e-42 153
Msa0806210 AT2G14530.1 30.607 379 196 17 74 419 62 406 1.27e-41 152
Msa0806210 AT3G62390.1 29.839 372 212 16 68 421 134 474 3.56e-41 152
Msa0806210 AT5G49340.1 27.473 364 224 9 74 419 100 441 1.15e-40 150
Msa0806210 AT2G42570.1 30.790 367 200 15 66 419 39 364 1.71e-39 145
Msa0806210 AT5G20680.6 27.154 383 211 10 68 419 112 457 2.35e-39 147
Msa0806210 AT5G20680.2 27.034 381 214 9 68 419 188 533 3.07e-39 148
Msa0806210 AT5G20680.4 27.034 381 214 9 68 419 206 551 3.59e-39 148
Msa0806210 AT5G20680.1 27.034 381 214 9 68 419 206 551 3.59e-39 148
Msa0806210 AT5G20680.3 27.034 381 214 9 68 419 206 551 3.59e-39 148
Msa0806210 AT5G20680.5 27.034 381 214 9 68 419 206 551 3.59e-39 148
Msa0806210 AT2G40160.1 29.793 386 222 18 57 415 56 419 3.82e-39 146
Msa0806210 AT2G40160.2 29.793 386 222 18 57 415 56 419 3.89e-39 146
Msa0806210 AT5G01360.2 28.533 368 232 13 63 416 25 375 1.53e-38 143
Msa0806210 AT3G55990.1 30.053 376 219 18 62 415 126 479 2.00e-38 145
Msa0806210 AT1G73140.1 28.877 374 216 15 63 415 57 401 3.32e-38 143
Msa0806210 AT1G60790.1 27.056 377 231 14 65 420 181 534 3.47e-38 145
Msa0806210 AT2G37720.1 28.721 383 203 11 69 419 138 482 3.47e-38 144
Msa0806210 AT5G01360.1 28.533 368 232 13 63 416 81 431 4.83e-38 143
Msa0806210 AT2G40150.1 29.235 366 215 16 74 415 71 416 1.00e-37 142
Msa0806210 AT5G19160.2 29.080 337 195 8 6 308 9 335 2.96e-36 136
Msa0806210 AT2G31110.2 29.700 367 200 16 64 415 35 358 3.96e-36 136
Msa0806210 AT2G38320.1 28.686 373 236 15 58 415 43 400 3.24e-35 135
Msa0806210 AT3G14850.2 31.476 359 195 15 74 419 34 354 3.93e-35 134
Msa0806210 AT3G11030.1 28.261 368 217 15 74 419 106 448 6.84e-35 135
Msa0806210 AT1G29050.1 29.268 369 211 15 62 419 49 378 7.81e-35 133
Msa0806210 AT2G30900.1 28.612 353 207 13 74 415 43 361 9.29e-35 133
Msa0806210 AT5G64470.3 28.108 370 214 15 73 419 54 394 4.76e-34 131
Msa0806210 AT2G40320.1 26.923 364 226 13 71 416 78 419 3.82e-33 129
Msa0806210 AT5G64470.2 27.660 376 214 16 73 419 54 400 1.54e-32 127
Msa0806210 AT3G54260.1 26.891 357 209 12 74 415 51 370 1.73e-31 124
Msa0806210 AT2G34070.1 27.520 367 213 15 66 419 57 383 1.07e-30 122
Msa0806210 AT1G78710.1 29.700 367 210 16 63 419 28 356 5.38e-30 119
Msa0806210 AT3G06080.1 30.522 249 147 6 74 308 103 339 6.67e-30 119
Msa0806210 AT3G62390.2 28.070 285 173 8 42 308 102 372 4.38e-29 118
Msa0806210 AT2G38320.2 28.834 326 203 14 104 415 7 317 1.22e-28 115
Msa0806210 AT2G40160.3 29.885 261 167 10 57 307 56 310 2.01e-28 114
Msa0806210 AT5G20590.2 35.233 193 108 6 74 250 141 332 1.65e-27 113
Msa0806210 AT5G06230.3 31.621 253 145 7 74 308 62 304 1.66e-27 111
Msa0806210 AT5G01620.2 26.503 366 231 11 70 419 106 449 1.88e-27 114
Msa0806210 AT5G01620.1 26.503 366 231 11 70 419 106 449 1.88e-27 114
Msa0806210 AT5G01620.3 26.503 366 231 11 70 419 114 457 2.61e-27 114
Msa0806210 AT5G58600.2 31.513 238 145 7 74 307 66 289 4.96e-26 107
Msa0806210 AT2G30900.2 36.628 172 92 6 74 238 43 204 1.20e-23 100
Msa0806210 AT2G31110.3 29.258 229 132 7 25 245 7 213 3.94e-23 98.6
Msa0806210 AT1G78710.2 35.754 179 103 6 56 225 9 184 8.42e-21 92.8
Msa0806210 AT5G64470.1 26.389 288 171 12 73 337 54 323 6.54e-20 90.5
Msa0806210 AT5G64470.4 27.372 274 158 12 73 323 54 309 3.88e-19 88.2

Find 131 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAATTTCTTGATGATTT+TGG 0.053119 5_3:+23734503 None:intergenic
GTCCTTAAAGCCTTTCTTAA+TGG 0.091385 5_3:+23732410 None:intergenic
TTCCATTAAGAAAGGCTTTA+AGG 0.121836 5_3:-23732412 Msa0806210:CDS
AAATGTATTGTATTAAAATT+TGG 0.148603 5_3:+23732608 None:intergenic
TTGATATGAAAATTTATATT+TGG 0.186932 5_3:-23734335 Msa0806210:five_prime_UTR
TCTACTCCTCCTCATTATTC+AGG 0.242819 5_3:-23733840 Msa0806210:intron
GGTATTGGATGTAACTAAAT+TGG 0.247978 5_3:-23732141 Msa0806210:CDS
GCCACCAATGTGAACCTTTA+TGG 0.250930 5_3:+23732711 None:intergenic
TGCTTAAATTGTTCCGGTTT+CGG 0.254274 5_3:-23732464 Msa0806210:CDS
CCTTTATGGTGGACAGGTTT+AGG 0.257428 5_3:+23732725 None:intergenic
CTTAGTTGGAGAAAAGTATT+TGG 0.257933 5_3:+23732836 None:intergenic
AGTAGAAGATGAAGTGTATA+TGG 0.261792 5_3:+23733954 None:intergenic
CACCAAGGACCTAGGTTTCT+TGG 0.263015 5_3:+23734385 None:intergenic
GGAAAGGGTGAGAAGAAAAT+TGG 0.278867 5_3:-23732254 Msa0806210:CDS
CACCAACAAATGTGCCATTA+TGG 0.283857 5_3:+23731843 None:intergenic
GGTCTTAATTACACTGATAT+TGG 0.285156 5_3:-23732443 Msa0806210:CDS
GTGACTACTTCAATGGAAAA+TGG 0.286087 5_3:-23733006 Msa0806210:CDS
TCTCTTGTATCCTTAATTCA+TGG 0.290646 5_3:-23734671 Msa0806210:five_prime_UTR
CAAACTACCTTTACTATAGA+TGG 0.299980 5_3:-23732892 Msa0806210:CDS
TGTGTTCATTGGTGTTTGCC+TGG 0.324131 5_3:-23732032 Msa0806210:CDS
CTTGGAAGTTTGCATTCATT+TGG 0.325913 5_3:+23732866 None:intergenic
AAAGAGAAATCCATGAATTA+AGG 0.326553 5_3:+23734661 None:intergenic
TCTAGACAAGGAAAATGTTT+GGG 0.327479 5_3:-23731794 Msa0806210:three_prime_UTR
TGCAAAGAAGAGACTCAAAT+TGG 0.329679 5_3:+23732768 None:intergenic
TTCCATAATGGCACATTTGT+TGG 0.341005 5_3:-23731845 Msa0806210:three_prime_UTR
AGGGATTAGATTTGAGGTAT+TGG 0.342670 5_3:-23732156 Msa0806210:CDS
ATCCTAAGTGTTTGGAATCA+TGG 0.351259 5_3:+23734553 None:intergenic
TTCTAGACAAGGAAAATGTT+TGG 0.354572 5_3:-23731795 Msa0806210:three_prime_UTR
GACTTGATTGTGTTATCGTT+TGG 0.361764 5_3:-23732542 Msa0806210:CDS
GTGTTGAGGAGGGAGGATTC+AGG 0.362658 5_3:+23734469 None:intergenic
TGTTCAAAGACTGAACCTTA+TGG 0.369816 5_3:-23732275 Msa0806210:CDS
TTAGGATGCTTAAATTGTTC+CGG 0.372597 5_3:-23732470 Msa0806210:CDS
CCTAAACCTGTCCACCATAA+AGG 0.373037 5_3:-23732725 Msa0806210:CDS
TCTCTGATCTTCTTCCATAA+TGG 0.379762 5_3:-23731857 Msa0806210:three_prime_UTR
AGATGATGAAGAAGTGGGAT+TGG 0.380315 5_3:-23731977 Msa0806210:CDS
TGTTCATGATCCTAAGTGTT+TGG 0.381279 5_3:+23734545 None:intergenic
TCATCACCTTGCACAAGAAA+TGG 0.391236 5_3:+23732647 None:intergenic
AAAGAATAAAGTACAATATA+CGG 0.393984 5_3:+23734262 None:intergenic
CCAAGATCATGTTGATTAAC+CGG 0.398828 5_3:-23734445 Msa0806210:five_prime_UTR
ACCAAGGACCTAGGTTTCTT+GGG 0.399875 5_3:+23734386 None:intergenic
AAATGGAAAGCGAGATTAGA+AGG 0.400157 5_3:-23732229 Msa0806210:CDS
CCTTCGGTTTATTATGAGAA+TGG 0.405122 5_3:-23732500 Msa0806210:CDS
AAAGTTTAATATCTACATAT+TGG 0.408158 5_3:+23731753 None:intergenic
TTGGTTTATTAATGTTCCTT+CGG 0.408464 5_3:-23732516 Msa0806210:CDS
TTCTTCTCACCCTTTCCATA+AGG 0.411285 5_3:+23732260 None:intergenic
CATGTTGTTCCATTGTATAA+AGG 0.411961 5_3:+23732965 None:intergenic
AAAGTATTTGGTTCAAACCT+TGG 0.422116 5_3:+23732848 None:intergenic
GGTTTATTATGAGAATGGTT+TGG 0.433915 5_3:-23732495 Msa0806210:CDS
TGCAAAATGATTGTGTTCAT+TGG 0.437679 5_3:-23732043 Msa0806210:CDS
GATGAAGTGTATATGGAAGA+AGG 0.439128 5_3:+23733961 None:intergenic
ACTTGGAGGGATTAGATTTG+AGG 0.443087 5_3:-23732162 Msa0806210:CDS
AGTGTAATTAAGACCGAAAC+CGG 0.443307 5_3:+23732451 None:intergenic
TTGTGTTATCGTTTGGCAAT+TGG 0.445306 5_3:-23732535 Msa0806210:CDS
GTTGCTAAAGGGAATGGAAT+TGG 0.445312 5_3:-23732359 Msa0806210:CDS
ACACCGTTAGCGAACGGAAA+AGG 0.447241 5_3:+23732077 None:intergenic
TGCACACTTATCTATATTTG+AGG 0.448253 5_3:+23734221 None:intergenic
AAGGATTGAGATTGAAGAAG+TGG 0.457929 5_3:-23732210 Msa0806210:CDS
CACCCATGTGACTACTTCAA+TGG 0.459804 5_3:-23733013 Msa0806210:CDS
CTATTCTATTCTATTCCACT+TGG 0.460694 5_3:-23734097 Msa0806210:five_prime_UTR
TAAGCACCTGGATGACCATC+TGG 0.468486 5_3:+23732107 None:intergenic
ATGTCTCACTCTTCACTCCT+TGG 0.471169 5_3:+23731945 None:intergenic
GATGAAGAAGTGGGATTGGA+AGG 0.471664 5_3:-23731973 Msa0806210:CDS
TTGAGAGGAAAGTTGCTAAA+GGG 0.472700 5_3:-23732370 Msa0806210:CDS
ATGCAACATTATCCGTGTAT+TGG 0.475380 5_3:-23732673 Msa0806210:CDS
AAGTATAAATAAAGAACAAT+GGG 0.476003 5_3:+23733915 None:intergenic
GGTGATGAAAGATCAAAATC+CGG 0.476546 5_3:-23732632 Msa0806210:CDS
TCTAATTTATGATTTAATTG+AGG 0.477409 5_3:+23734415 None:intergenic
ATCTCATTCCAAGTATCTAT+AGG 0.478611 5_3:+23732008 None:intergenic
TTGAGACCAGATGGTCATCC+AGG 0.479603 5_3:-23732113 Msa0806210:CDS
GTGTTCATTGGTGTTTGCCT+GGG 0.480611 5_3:-23732031 Msa0806210:CDS
TGTGAACCTTTATGGTGGAC+AGG 0.481848 5_3:+23732719 None:intergenic
TGCCTGGGCCTATAGATACT+TGG 0.482132 5_3:-23732016 Msa0806210:CDS
CCGGTTAATCAACATGATCT+TGG 0.485376 5_3:+23734445 None:intergenic
TAGTAAAGGTAGTTTGAGTC+AGG 0.487516 5_3:+23732899 None:intergenic
ACCATAAAGGTTCACATTGG+TGG 0.496277 5_3:-23732712 Msa0806210:CDS
TCTAGAGATGATGAAGAAGT+GGG 0.498464 5_3:-23731982 Msa0806210:CDS
TTGGTTTCCATCTATAGTAA+AGG 0.502522 5_3:+23732885 None:intergenic
TTTGGGGTTTGAAAGCAACT+TGG 0.510275 5_3:-23731777 Msa0806210:three_prime_UTR
TAATAAATTAACAAGCTAAG+AGG 0.510694 5_3:+23731921 None:intergenic
AAAGTATAAATAAAGAACAA+TGG 0.511075 5_3:+23733914 None:intergenic
GATGGTGATTGGGATAAGGC+TGG 0.512386 5_3:-23732302 Msa0806210:CDS
GTTTGAGTCAGGTCTTCCAT+TGG 0.512588 5_3:+23732910 None:intergenic
TTCTAGAGATGATGAAGAAG+TGG 0.512711 5_3:-23731983 Msa0806210:CDS
CAAATGAATGCAAACTTCCA+AGG 0.514979 5_3:-23732865 Msa0806210:CDS
AAGACTGAACCTTATGGAAA+GGG 0.515408 5_3:-23732269 Msa0806210:CDS
CATAATGGCACATTTGTTGG+TGG 0.515551 5_3:-23731842 Msa0806210:three_prime_UTR
AGGAAAGTTGCTAAAGGGAA+TGG 0.518826 5_3:-23732365 Msa0806210:CDS
ACCCAAGAAACCTAGGTCCT+TGG 0.522176 5_3:-23734387 Msa0806210:five_prime_UTR
TATTGTATTAAAATTTGGCC+CGG 0.524973 5_3:+23732613 None:intergenic
TGGTCGCCATTTCTTGTGCA+AGG 0.526450 5_3:-23732653 Msa0806210:CDS
AGCAGAAAACAAAGTAGGAA+AGG 0.526927 5_3:-23734051 Msa0806210:five_prime_UTR
ATTGAGAGGAAAGTTGCTAA+AGG 0.529202 5_3:-23732371 Msa0806210:CDS
TATTCTATTCTATTCCACTT+GGG 0.540942 5_3:-23734096 Msa0806210:five_prime_UTR
ATGAAGTGTATATGGAAGAA+GGG 0.544510 5_3:+23733962 None:intergenic
GTGATGAAAGATCAAAATCC+GGG 0.544715 5_3:-23732631 Msa0806210:CDS
AAAGACTGAACCTTATGGAA+AGG 0.548583 5_3:-23732270 Msa0806210:CDS
TGAAATAAAATTTAAACTAG+AGG 0.550858 5_3:+23734307 None:intergenic
ATAATGGCACATTTGTTGGT+GGG 0.555933 5_3:-23731841 Msa0806210:three_prime_UTR
GATGTTAGCAAGTTTAACAA+AGG 0.558526 5_3:+23734636 None:intergenic
TTCCAAGTATCTATAGGCCC+AGG 0.563711 5_3:+23732014 None:intergenic
TTATACAATGGAACAACATG+TGG 0.569031 5_3:-23732962 Msa0806210:CDS
TGAATTAAGGATACAAGAGA+TGG 0.569147 5_3:+23734674 None:intergenic
GGACAAAATTGTATCACCAA+TGG 0.575587 5_3:-23732926 Msa0806210:CDS
CTATGAGCAGAAAACAAAGT+AGG 0.577669 5_3:-23734056 Msa0806210:five_prime_UTR
TTGGCATTGTTGAGACCAGA+TGG 0.580975 5_3:-23732122 Msa0806210:CDS
ATGAAGAAGTGGGATTGGAA+GGG 0.584115 5_3:-23731972 Msa0806210:CDS
GAGTATAACCCAAGAAACCT+AGG 0.593290 5_3:-23734394 Msa0806210:five_prime_UTR
CTAGACAAGGAAAATGTTTG+GGG 0.599938 5_3:-23731793 Msa0806210:three_prime_UTR
ATGATCTTGGTGTGTTGAGG+AGG 0.601958 5_3:+23734458 None:intergenic
TTCCATTGAAGTAGTCACAT+GGG 0.602933 5_3:+23733011 None:intergenic
AACCATGATTCCAAACACTT+AGG 0.604706 5_3:-23734555 Msa0806210:five_prime_UTR
TCTTGGTGTGTTGAGGAGGG+AGG 0.606448 5_3:+23734462 None:intergenic
TTTCCATTGAAGTAGTCACA+TGG 0.607443 5_3:+23733010 None:intergenic
CAATCACCATCAAAATGAGA+AGG 0.607997 5_3:+23732314 None:intergenic
ATTAACATACCTGAATAATG+AGG 0.612033 5_3:+23733831 None:intergenic
AAGAAATGGCGACCAATACA+CGG 0.614637 5_3:+23732661 None:intergenic
AAGTGCGACACCAAGGACCT+AGG 0.616652 5_3:+23734377 None:intergenic
TTCGGAACACCGTTAGCGAA+CGG 0.617341 5_3:+23732071 None:intergenic
AACATGATCTTGGTGTGTTG+AGG 0.620460 5_3:+23734455 None:intergenic
AAAGGATTCATATAAGCACC+TGG 0.621291 5_3:+23732095 None:intergenic
ATTTAATTGAGGAAACTCAC+CGG 0.624932 5_3:+23734426 None:intergenic
GTCTTAATAGCATAATTGAG+AGG 0.633582 5_3:-23732385 Msa0806210:CDS
GGGATTGGAAGGGACAGCCA+AGG 0.651431 5_3:-23731962 Msa0806210:CDS
CCATTCTCATAATAAACCGA+AGG 0.655142 5_3:+23732500 None:intergenic
AACATACCTGAATAATGAGG+AGG 0.659116 5_3:+23733834 None:intergenic
AAATTGGCGAAGGATATGGA+TGG 0.663011 5_3:-23732569 Msa0806210:CDS
ACATGTGGAACAATCAAACA+AGG 0.664368 5_3:-23732947 Msa0806210:CDS
TTGAAGAAAGTGCGACACCA+AGG 0.664614 5_3:+23734370 None:intergenic
TCCACCATAAAGGTTCACAT+TGG 0.669651 5_3:-23732715 Msa0806210:CDS
TGATCTTGGTGTGTTGAGGA+GGG 0.671076 5_3:+23734459 None:intergenic
ACCAATGTGAACCTTTATGG+TGG 0.671348 5_3:+23732714 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAAAAGTTAAGAT+TGG + chr5_3:23731709-23731728 None:intergenic 10.0%
!!! AAATATAAATTTTCATATCA+AGG + chr5_3:23731974-23731993 None:intergenic 10.0%
!! TTGATATGAAAATTTATATT+TGG - chr5_3:23731973-23731992 Msa0806210:CDS 10.0%
!! AAATGTATTGTATTAAAATT+TGG + chr5_3:23733703-23733722 None:intergenic 10.0%
!!! AAATTTTAATACAATACATT+TGG - chr5_3:23733702-23733721 Msa0806210:intron 10.0%
!!! TAATAAACATAATCTTTTTA+TGG + chr5_3:23734424-23734443 None:intergenic 10.0%
!!! AAATCAGTTTTTAATTTTTA+TGG + chr5_3:23734615-23734634 None:intergenic 10.0%
!! TCTAATTTATGATTTAATTG+AGG + chr5_3:23731896-23731915 None:intergenic 15.0%
!! TGAAATAAAATTTAAACTAG+AGG + chr5_3:23732004-23732023 None:intergenic 15.0%
!! AAAGAATAAAGTACAATATA+CGG + chr5_3:23732049-23732068 None:intergenic 15.0%
!! AAGTATAAATAAAGAACAAT+GGG + chr5_3:23732396-23732415 None:intergenic 15.0%
!! AAAGTATAAATAAAGAACAA+TGG + chr5_3:23732397-23732416 None:intergenic 15.0%
!!! TTTTTAAAGGAAAAATGATT+AGG - chr5_3:23732588-23732607 Msa0806210:CDS 15.0%
!! GTACTATATTTAATTAAATG+AGG - chr5_3:23732700-23732719 Msa0806210:CDS 15.0%
!! AAAGTTTAATATCTACATAT+TGG + chr5_3:23734558-23734577 None:intergenic 15.0%
!!! ATTTTTGATTAGATGAATTA+TGG - chr5_3:23734637-23734656 Msa0806210:five_prime_UTR 15.0%
!!! ATGATTAGGTGTTTTTTTAA+AGG - chr5_3:23732575-23732594 Msa0806210:CDS 20.0%
!! TAATTAAATGAGGTAATCAT+TGG - chr5_3:23732710-23732729 Msa0806210:CDS 20.0%
!! AATATATCTTTCGATAAATC+TGG + chr5_3:23732929-23732948 None:intergenic 20.0%
!!! CTTTTTATAAGTGTCTTATA+AGG - chr5_3:23733135-23733154 Msa0806210:intron 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG - chr5_3:23733888-23733907 Msa0806210:CDS 20.0%
!! TAATAAATTAACAAGCTAAG+AGG + chr5_3:23734390-23734409 None:intergenic 20.0%
! AAAGAGAAATCCATGAATTA+AGG + chr5_3:23731650-23731669 None:intergenic 25.0%
!!! CACAAATTTCTTGATGATTT+TGG + chr5_3:23731808-23731827 None:intergenic 25.0%
!!! TTTACTTCACGCAAATTTTT+TGG - chr5_3:23732179-23732198 Msa0806210:CDS 25.0%
! TATTCTATTCTATTCCACTT+GGG - chr5_3:23732212-23732231 Msa0806210:CDS 25.0%
! ATTAACATACCTGAATAATG+AGG + chr5_3:23732480-23732499 None:intergenic 25.0%
! ATTCACACTTGAAAATGATT+AGG - chr5_3:23732561-23732580 Msa0806210:CDS 25.0%
! TGTTGAATTGAACACTAAAA+TGG - chr5_3:23732613-23732632 Msa0806210:CDS 25.0%
! TTCAACTACTCTAATTAACA+TGG + chr5_3:23732658-23732677 None:intergenic 25.0%
! TTAAACACTTATAGTGAGAA+TGG - chr5_3:23732792-23732811 Msa0806210:CDS 25.0%
! TTAACAAATCACCACAATTA+AGG - chr5_3:23732826-23732845 Msa0806210:CDS 25.0%
! TATCGAAAGATATATTTCGA+TGG - chr5_3:23732933-23732952 Msa0806210:CDS 25.0%
!!! TTTTAACTCTTTTTGTCCAT+CGG - chr5_3:23732962-23732981 Msa0806210:CDS 25.0%
! TATAAGTGTCTTATAAGGAT+TGG - chr5_3:23733140-23733159 Msa0806210:intron 25.0%
!!! TTGTATATCAGAGGTTTTAA+AGG + chr5_3:23733167-23733186 None:intergenic 25.0%
!! AAATGGATTCATGACAAAAA+AGG - chr5_3:23733319-23733338 Msa0806210:intron 25.0%
! AAAAAAGGTCCTTTATACAA+TGG - chr5_3:23733334-23733353 Msa0806210:intron 25.0%
!! AACAAACACATTTCTTTTGT+TGG - chr5_3:23733499-23733518 Msa0806210:intron 25.0%
! TATTGTATTAAAATTTGGCC+CGG + chr5_3:23733698-23733717 None:intergenic 25.0%
! TTGGTTTATTAATGTTCCTT+CGG - chr5_3:23733792-23733811 Msa0806210:intron 25.0%
! TGAGAAGAAAATTGGAAAAA+TGG - chr5_3:23734062-23734081 Msa0806210:five_prime_UTR 25.0%
! AAGAAAAAAGCAAATGAACT+TGG - chr5_3:23734129-23734148 Msa0806210:five_prime_UTR 25.0%
TGAATTAAGGATACAAGAGA+TGG + chr5_3:23731637-23731656 None:intergenic 30.0%
TCTCTTGTATCCTTAATTCA+TGG - chr5_3:23731637-23731656 Msa0806210:three_prime_UTR 30.0%
GATGTTAGCAAGTTTAACAA+AGG + chr5_3:23731675-23731694 None:intergenic 30.0%
!! TTCTTGATGATTTTGGAGAA+AGG + chr5_3:23731801-23731820 None:intergenic 30.0%
ATTTAATTGAGGAAACTCAC+CGG + chr5_3:23731885-23731904 None:intergenic 30.0%
TGCACACTTATCTATATTTG+AGG + chr5_3:23732090-23732109 None:intergenic 30.0%
CTATTCTATTCTATTCCACT+TGG - chr5_3:23732211-23732230 Msa0806210:CDS 30.0%
ATGAAGTGTATATGGAAGAA+GGG + chr5_3:23732349-23732368 None:intergenic 30.0%
AGTAGAAGATGAAGTGTATA+TGG + chr5_3:23732357-23732376 None:intergenic 30.0%
! GAGATTTTGTAAGAGACAAA+GGG + chr5_3:23732422-23732441 None:intergenic 30.0%
! TGAGATTTTGTAAGAGACAA+AGG + chr5_3:23732423-23732442 None:intergenic 30.0%
TATCTTTCGATAAATCTGGA+GGG + chr5_3:23732925-23732944 None:intergenic 30.0%
ATATCTTTCGATAAATCTGG+AGG + chr5_3:23732926-23732945 None:intergenic 30.0%
AACCATCTACAAAATTGTCA+CGG - chr5_3:23733044-23733063 Msa0806210:intron 30.0%
! TAAAAAGTGCAAAGAGCTTT+CGG + chr5_3:23733122-23733141 None:intergenic 30.0%
AAACCTCTGATATACAATCA+TGG - chr5_3:23733170-23733189 Msa0806210:intron 30.0%
CATGTTGTTCCATTGTATAA+AGG + chr5_3:23733346-23733365 None:intergenic 30.0%
TTATACAATGGAACAACATG+TGG - chr5_3:23733346-23733365 Msa0806210:intron 30.0%
CAAACTACCTTTACTATAGA+TGG - chr5_3:23733416-23733435 Msa0806210:intron 30.0%
TTGGTTTCCATCTATAGTAA+AGG + chr5_3:23733426-23733445 None:intergenic 30.0%
! AAAGTATTTGGTTCAAACCT+TGG + chr5_3:23733463-23733482 None:intergenic 30.0%
!! CTTAGTTGGAGAAAAGTATT+TGG + chr5_3:23733475-23733494 None:intergenic 30.0%
!!! TGTGTTTGTTTTTGCTTAGT+TGG + chr5_3:23733489-23733508 None:intergenic 30.0%
GGTTTATTATGAGAATGGTT+TGG - chr5_3:23733813-23733832 Msa0806210:intron 30.0%
!!! TATGAGAATGGTTTGGTTTT+AGG - chr5_3:23733820-23733839 Msa0806210:intron 30.0%
TTAGGATGCTTAAATTGTTC+CGG - chr5_3:23733838-23733857 Msa0806210:intron 30.0%
GGTCTTAATTACACTGATAT+TGG - chr5_3:23733865-23733884 Msa0806210:CDS 30.0%
TTCCATTAAGAAAGGCTTTA+AGG - chr5_3:23733896-23733915 Msa0806210:CDS 30.0%
GTCTTAATAGCATAATTGAG+AGG - chr5_3:23733923-23733942 Msa0806210:CDS 30.0%
! TTTTCACCTTCTCATTTTGA+TGG - chr5_3:23733988-23734007 Msa0806210:CDS 30.0%
!!! TTCTCATTTTGATGGTGATT+GGG - chr5_3:23733996-23734015 Msa0806210:CDS 30.0%
AAAAAAGCAAATGAACTTGG+AGG - chr5_3:23734132-23734151 Msa0806210:five_prime_UTR 30.0%
AAAAAGCAAATGAACTTGGA+GGG - chr5_3:23734133-23734152 Msa0806210:five_prime_UTR 30.0%
! GGTATTGGATGTAACTAAAT+TGG - chr5_3:23734167-23734186 Msa0806210:five_prime_UTR 30.0%
! CAATCATTTTGCACATACTT+CGG + chr5_3:23734258-23734277 None:intergenic 30.0%
TGCAAAATGATTGTGTTCAT+TGG - chr5_3:23734265-23734284 Msa0806210:five_prime_UTR 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5_3:23734303-23734322 None:intergenic 30.0%
!!! TTGGTTTTAGTTTCTAGACA+AGG - chr5_3:23734502-23734521 Msa0806210:five_prime_UTR 30.0%
TTCTAGACAAGGAAAATGTT+TGG - chr5_3:23734513-23734532 Msa0806210:five_prime_UTR 30.0%
TCTAGACAAGGAAAATGTTT+GGG - chr5_3:23734514-23734533 Msa0806210:five_prime_UTR 30.0%
AACCATGATTCCAAACACTT+AGG - chr5_3:23731753-23731772 Msa0806210:three_prime_UTR 35.0%
! ATCCTAAGTGTTTGGAATCA+TGG + chr5_3:23731758-23731777 None:intergenic 35.0%
TGTTCATGATCCTAAGTGTT+TGG + chr5_3:23731766-23731785 None:intergenic 35.0%
!! GATTTTGGAGAAAGGAGTTT+TGG + chr5_3:23731793-23731812 None:intergenic 35.0%
! CCAAGATCATGTTGATTAAC+CGG - chr5_3:23731863-23731882 Msa0806210:three_prime_UTR 35.0%
CTATGAGCAGAAAACAAAGT+AGG - chr5_3:23732252-23732271 Msa0806210:CDS 35.0%
AGCAGAAAACAAAGTAGGAA+AGG - chr5_3:23732257-23732276 Msa0806210:CDS 35.0%
GATGAAGTGTATATGGAAGA+AGG + chr5_3:23732350-23732369 None:intergenic 35.0%
AACATACCTGAATAATGAGG+AGG + chr5_3:23732477-23732496 None:intergenic 35.0%
TCATAACACCTCATGATATC+CGG - chr5_3:23732858-23732877 Msa0806210:CDS 35.0%
TAAAGAGCAACTACCTATAC+CGG + chr5_3:23732880-23732899 None:intergenic 35.0%
!!! TTCCAGTTTTAAGCAACACA+TGG - chr5_3:23733009-23733028 Msa0806210:CDS 35.0%
!! TTCCATGTGTTGCTTAAAAC+TGG + chr5_3:23733014-23733033 None:intergenic 35.0%
TTTGTAGATGGTTGCTCTTT+AGG + chr5_3:23733037-23733056 None:intergenic 35.0%
! AACCGTGACAATTTTGTAGA+TGG + chr5_3:23733049-23733068 None:intergenic 35.0%
! ACAAAATTGTCACGGTTTTG+AGG - chr5_3:23733052-23733071 Msa0806210:intron 35.0%
ACACCATGATTGTATATCAG+AGG + chr5_3:23733176-23733195 None:intergenic 35.0%
TTCCATTGAAGTAGTCACAT+GGG + chr5_3:23733300-23733319 None:intergenic 35.0%
TTTCCATTGAAGTAGTCACA+TGG + chr5_3:23733301-23733320 None:intergenic 35.0%
GTGACTACTTCAATGGAAAA+TGG - chr5_3:23733302-23733321 Msa0806210:intron 35.0%
ACATGTGGAACAATCAAACA+AGG - chr5_3:23733361-23733380 Msa0806210:intron 35.0%
GGACAAAATTGTATCACCAA+TGG - chr5_3:23733382-23733401 Msa0806210:intron 35.0%
TAGTAAAGGTAGTTTGAGTC+AGG + chr5_3:23733412-23733431 None:intergenic 35.0%
CTTGGAAGTTTGCATTCATT+TGG + chr5_3:23733445-23733464 None:intergenic 35.0%
CAAATGAATGCAAACTTCCA+AGG - chr5_3:23733443-23733462 Msa0806210:intron 35.0%
TGCAAAGAAGAGACTCAAAT+TGG + chr5_3:23733543-23733562 None:intergenic 35.0%
ATGCAACATTATCCGTGTAT+TGG - chr5_3:23733635-23733654 Msa0806210:intron 35.0%
GGTGATGAAAGATCAAAATC+CGG - chr5_3:23733676-23733695 Msa0806210:intron 35.0%
GTGATGAAAGATCAAAATCC+GGG - chr5_3:23733677-23733696 Msa0806210:intron 35.0%
GGATCGCGTTAATGAAAAAT+TGG - chr5_3:23733723-23733742 Msa0806210:intron 35.0%
CGTTAATGAAAAATTGGCGA+AGG - chr5_3:23733729-23733748 Msa0806210:intron 35.0%
TGAAAAATTGGCGAAGGATA+TGG - chr5_3:23733735-23733754 Msa0806210:intron 35.0%
GACTTGATTGTGTTATCGTT+TGG - chr5_3:23733766-23733785 Msa0806210:intron 35.0%
TTGTGTTATCGTTTGGCAAT+TGG - chr5_3:23733773-23733792 Msa0806210:intron 35.0%
CCATTCTCATAATAAACCGA+AGG + chr5_3:23733811-23733830 None:intergenic 35.0%
CCTTCGGTTTATTATGAGAA+TGG - chr5_3:23733808-23733827 Msa0806210:intron 35.0%
TGCTTAAATTGTTCCGGTTT+CGG - chr5_3:23733844-23733863 Msa0806210:CDS 35.0%
AGTGTAATTAAGACCGAAAC+CGG + chr5_3:23733860-23733879 None:intergenic 35.0%
GTCCTTAAAGCCTTTCTTAA+TGG + chr5_3:23733901-23733920 None:intergenic 35.0%
ATTGAGAGGAAAGTTGCTAA+AGG - chr5_3:23733937-23733956 Msa0806210:CDS 35.0%
TTGAGAGGAAAGTTGCTAAA+GGG - chr5_3:23733938-23733957 Msa0806210:CDS 35.0%
CAATCACCATCAAAATGAGA+AGG + chr5_3:23733997-23734016 None:intergenic 35.0%
!!! CTTCTCATTTTGATGGTGAT+TGG - chr5_3:23733995-23734014 Msa0806210:CDS 35.0%
!!! TTTTGATGGTGATTGGGATA+AGG - chr5_3:23734002-23734021 Msa0806210:CDS 35.0%
TGTTCAAAGACTGAACCTTA+TGG - chr5_3:23734033-23734052 Msa0806210:five_prime_UTR 35.0%
AAAGACTGAACCTTATGGAA+AGG - chr5_3:23734038-23734057 Msa0806210:five_prime_UTR 35.0%
AAGACTGAACCTTATGGAAA+GGG - chr5_3:23734039-23734058 Msa0806210:five_prime_UTR 35.0%
AAATGGAAAGCGAGATTAGA+AGG - chr5_3:23734079-23734098 Msa0806210:five_prime_UTR 35.0%
AAGGATTGAGATTGAAGAAG+TGG - chr5_3:23734098-23734117 Msa0806210:five_prime_UTR 35.0%
! AGGGATTAGATTTGAGGTAT+TGG - chr5_3:23734152-23734171 Msa0806210:five_prime_UTR 35.0%
AAAGGATTCATATAAGCACC+TGG + chr5_3:23734216-23734235 None:intergenic 35.0%
! TTCTAGAGATGATGAAGAAG+TGG - chr5_3:23734325-23734344 Msa0806210:five_prime_UTR 35.0%
TCTAGAGATGATGAAGAAGT+GGG - chr5_3:23734326-23734345 Msa0806210:five_prime_UTR 35.0%
TCTCTGATCTTCTTCCATAA+TGG - chr5_3:23734451-23734470 Msa0806210:five_prime_UTR 35.0%
TTCCATAATGGCACATTTGT+TGG - chr5_3:23734463-23734482 Msa0806210:five_prime_UTR 35.0%
! ATAATGGCACATTTGTTGGT+GGG - chr5_3:23734467-23734486 Msa0806210:five_prime_UTR 35.0%
CTAGACAAGGAAAATGTTTG+GGG - chr5_3:23734515-23734534 Msa0806210:five_prime_UTR 35.0%
! AACATGATCTTGGTGTGTTG+AGG + chr5_3:23731856-23731875 None:intergenic 40.0%
CCGGTTAATCAACATGATCT+TGG + chr5_3:23731866-23731885 None:intergenic 40.0%
GAGTATAACCCAAGAAACCT+AGG - chr5_3:23731914-23731933 Msa0806210:three_prime_UTR 40.0%
TCTACTCCTCCTCATTATTC+AGG - chr5_3:23732468-23732487 Msa0806210:CDS 40.0%
ATCACCACAATTAAGGCCTT+TGG - chr5_3:23732833-23732852 Msa0806210:CDS 40.0%
! ATGAGGTGTTATGAGACCAA+AGG + chr5_3:23732852-23732871 None:intergenic 40.0%
TACCTATACCGGATATCATG+AGG + chr5_3:23732869-23732888 None:intergenic 40.0%
CATCGAAACATATACTCCGA+TGG + chr5_3:23732981-23733000 None:intergenic 40.0%
CATCGGAGTATATGTTTCGA+TGG - chr5_3:23732979-23732998 Msa0806210:CDS 40.0%
!!! TTGTCACGGTTTTGAGGTTT+TGG - chr5_3:23733058-23733077 Msa0806210:intron 40.0%
!!! TGTCACGGTTTTGAGGTTTT+GGG - chr5_3:23733059-23733078 Msa0806210:intron 40.0%
TCCACCATAAAGGTTCACAT+TGG - chr5_3:23733593-23733612 Msa0806210:intron 40.0%
ACCAATGTGAACCTTTATGG+TGG + chr5_3:23733597-23733616 None:intergenic 40.0%
ACCATAAAGGTTCACATTGG+TGG - chr5_3:23733596-23733615 Msa0806210:intron 40.0%
AAGAAATGGCGACCAATACA+CGG + chr5_3:23733650-23733669 None:intergenic 40.0%
TCATCACCTTGCACAAGAAA+TGG + chr5_3:23733664-23733683 None:intergenic 40.0%
AAATTGGCGAAGGATATGGA+TGG - chr5_3:23733739-23733758 Msa0806210:intron 40.0%
AGGAAAGTTGCTAAAGGGAA+TGG - chr5_3:23733943-23733962 Msa0806210:CDS 40.0%
GTTGCTAAAGGGAATGGAAT+TGG - chr5_3:23733949-23733968 Msa0806210:CDS 40.0%
TTCTTCTCACCCTTTCCATA+AGG + chr5_3:23734051-23734070 None:intergenic 40.0%
GGAAAGGGTGAGAAGAAAAT+TGG - chr5_3:23734054-23734073 Msa0806210:five_prime_UTR 40.0%
ACTTGGAGGGATTAGATTTG+AGG - chr5_3:23734146-23734165 Msa0806210:five_prime_UTR 40.0%
! AATCCTTTTCCGTTCGCTAA+CGG - chr5_3:23734228-23734247 Msa0806210:five_prime_UTR 40.0%
AGATGATGAAGAAGTGGGAT+TGG - chr5_3:23734331-23734350 Msa0806210:five_prime_UTR 40.0%
ATGAAGAAGTGGGATTGGAA+GGG - chr5_3:23734336-23734355 Msa0806210:five_prime_UTR 40.0%
! CACCAACAAATGTGCCATTA+TGG + chr5_3:23734468-23734487 None:intergenic 40.0%
! CATAATGGCACATTTGTTGG+TGG - chr5_3:23734466-23734485 Msa0806210:five_prime_UTR 40.0%
!!! TGGTGGGAGACTTTTGTTTT+TGG - chr5_3:23734483-23734502 Msa0806210:five_prime_UTR 40.0%
! TTTGGGGTTTGAAAGCAACT+TGG - chr5_3:23734531-23734550 Msa0806210:five_prime_UTR 40.0%
! TGATCTTGGTGTGTTGAGGA+GGG + chr5_3:23731852-23731871 None:intergenic 45.0%
! ATGATCTTGGTGTGTTGAGG+AGG + chr5_3:23731853-23731872 None:intergenic 45.0%
ACCAAGGACCTAGGTTTCTT+GGG + chr5_3:23731925-23731944 None:intergenic 45.0%
! TTGAAGAAAGTGCGACACCA+AGG + chr5_3:23731941-23731960 None:intergenic 45.0%
! CTCTTTTGTGATGTCCCAAG+TGG + chr5_3:23732229-23732248 None:intergenic 45.0%
GAGACCAAAGGCCTTAATTG+TGG + chr5_3:23732840-23732859 None:intergenic 45.0%
CACCTCATGATATCCGGTAT+AGG - chr5_3:23732864-23732883 Msa0806210:CDS 45.0%
CACCCATGTGACTACTTCAA+TGG - chr5_3:23733295-23733314 Msa0806210:intron 45.0%
GTTTGAGTCAGGTCTTCCAT+TGG + chr5_3:23733401-23733420 None:intergenic 45.0%
!! CCTTTATGGTGGACAGGTTT+AGG + chr5_3:23733586-23733605 None:intergenic 45.0%
CCTAAACCTGTCCACCATAA+AGG - chr5_3:23733583-23733602 Msa0806210:intron 45.0%
!! TGTGAACCTTTATGGTGGAC+AGG + chr5_3:23733592-23733611 None:intergenic 45.0%
GCCACCAATGTGAACCTTTA+TGG + chr5_3:23733600-23733619 None:intergenic 45.0%
TTGGCATTGTTGAGACCAGA+TGG - chr5_3:23734186-23734205 Msa0806210:five_prime_UTR 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG - chr5_3:23734276-23734295 Msa0806210:five_prime_UTR 45.0%
! GTGTTCATTGGTGTTTGCCT+GGG - chr5_3:23734277-23734296 Msa0806210:five_prime_UTR 45.0%
TTCCAAGTATCTATAGGCCC+AGG + chr5_3:23734297-23734316 None:intergenic 45.0%
GATGAAGAAGTGGGATTGGA+AGG - chr5_3:23734335-23734354 Msa0806210:five_prime_UTR 45.0%
ATGTCTCACTCTTCACTCCT+TGG + chr5_3:23734366-23734385 None:intergenic 45.0%
!!! TTACTATTTTAATATATTAT+TGG + chr5_3:23734678-23734697 None:intergenic 5.0%
ACCCAAGAAACCTAGGTCCT+TGG - chr5_3:23731921-23731940 Msa0806210:three_prime_UTR 50.0%
CACCAAGGACCTAGGTTTCT+TGG + chr5_3:23731926-23731945 None:intergenic 50.0%
GGGCTTCGTAGCAATAAGGA+GGG + chr5_3:23732905-23732924 None:intergenic 50.0%
AGGGCTTCGTAGCAATAAGG+AGG + chr5_3:23732906-23732925 None:intergenic 50.0%
TGGAGGGCTTCGTAGCAATA+AGG + chr5_3:23732909-23732928 None:intergenic 50.0%
!!! ACGGTTTTGAGGTTTTGGGC+CGG - chr5_3:23733063-23733082 Msa0806210:intron 50.0%
!!! TGAGGTTTTGGGCCGGTTAA+TGG - chr5_3:23733070-23733089 Msa0806210:intron 50.0%
!!! GAGGTTTTGGGCCGGTTAAT+GGG - chr5_3:23733071-23733090 Msa0806210:intron 50.0%
TAACTAAACTCCGAGAGCCG+AGG + chr5_3:23733097-23733116 None:intergenic 50.0%
TGGTCGCCATTTCTTGTGCA+AGG - chr5_3:23733655-23733674 Msa0806210:intron 50.0%
GATGGTGATTGGGATAAGGC+TGG - chr5_3:23734006-23734025 Msa0806210:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5_3:23734195-23734214 Msa0806210:five_prime_UTR 50.0%
TAAGCACCTGGATGACCATC+TGG + chr5_3:23734204-23734223 None:intergenic 50.0%
ACACCGTTAGCGAACGGAAA+AGG + chr5_3:23734234-23734253 None:intergenic 50.0%
TTCGGAACACCGTTAGCGAA+CGG + chr5_3:23734240-23734259 None:intergenic 50.0%
TGCCTGGGCCTATAGATACT+TGG - chr5_3:23734292-23734311 Msa0806210:five_prime_UTR 50.0%
GTGTTGAGGAGGGAGGATTC+AGG + chr5_3:23731842-23731861 None:intergenic 55.0%
! TCTTGGTGTGTTGAGGAGGG+AGG + chr5_3:23731849-23731868 None:intergenic 55.0%
AAGTGCGACACCAAGGACCT+AGG + chr5_3:23731934-23731953 None:intergenic 55.0%
! TTGGGCCGGTTAATGGGCCT+CGG - chr5_3:23733077-23733096 Msa0806210:intron 60.0%
GAGAGCCGAGGCCCATTAAC+CGG + chr5_3:23733085-23733104 None:intergenic 60.0%
! GGTTAATGGGCCTCGGCTCT+CGG - chr5_3:23733084-23733103 Msa0806210:intron 60.0%
GGGATTGGAAGGGACAGCCA+AGG - chr5_3:23734346-23734365 Msa0806210:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 23731621 23734709 23731621 ID=Msa0806210;Name=Msa0806210
chr5_3 mRNA 23731621 23734709 23731621 ID=Msa0806210-mRNA-1;Parent=Msa0806210;Name=Msa0806210-mRNA-1;_AED=0.00;_eAED=0.00;_QI=682|1|1|1|1|1|2|329|432
chr5_3 exon 23731621 23733061 23731621 ID=Msa0806210-mRNA-1:exon:9314;Parent=Msa0806210-mRNA-1
chr5_3 exon 23733841 23734709 23733841 ID=Msa0806210-mRNA-1:exon:9313;Parent=Msa0806210-mRNA-1
chr5_3 five_prime_UTR 23734028 23734709 23734028 ID=Msa0806210-mRNA-1:five_prime_utr;Parent=Msa0806210-mRNA-1
chr5_3 CDS 23733841 23734027 23733841 ID=Msa0806210-mRNA-1:cds;Parent=Msa0806210-mRNA-1
chr5_3 CDS 23731950 23733061 23731950 ID=Msa0806210-mRNA-1:cds;Parent=Msa0806210-mRNA-1
chr5_3 three_prime_UTR 23731621 23731949 23731621 ID=Msa0806210-mRNA-1:three_prime_utr;Parent=Msa0806210-mRNA-1
Gene Sequence

>Msa0806210

ATGAAATGTACAATGAAAGATGATTCAATTTCACTCTCTAAGACCCTTCTTCCATATACACTTCATCTTCTACTTCTCATAGCTCTCTTACCCATTGTTCTTTATTTATACTTTTACCCTTTGTCTCTTACAAAATCTCAAACTCAGCTTCATCATGCAACTATTTCTACTCCTCCTCATTATTCAGTAAATGACAAACACAAAATTGATGAGCACCCATGTGACTACTTCAATGGAAAATGGATTCATGACAAAAAAGGTCCTTTATACAATGGAACAACATGTGGAACAATCAAACAAGGACAAAATTGTATCACCAATGGAAGACCTGACTCAAACTACCTTTACTATAGATGGAAACCAAATGAATGCAAACTTCCAAGGTTTGAACCAAATACTTTTCTCCAACTAAGCAAAAACAAACACATTTCTTTTGTTGGTGATTCTTTAGCTAGAAACCAATTTGAGTCTCTTCTTTGCATGTTAGCAACTGTTTCAAAACCTAAACCTGTCCACCATAAAGGTTCACATTGGTGGCATTTTTCTTCTCATAATGCAACATTATCCGTGTATTGGTCGCCATTTCTTGTGCAAGGTGATGAAAGATCAAAATCCGGGCCAAATTTTAATACAATACATTTGGATCGCGTTAATGAAAAATTGGCGAAGGATATGGATGGAATCGACTTGATTGTGTTATCGTTTGGCAATTGGTTTATTAATGTTCCTTCGGTTTATTATGAGAATGGTTTGGTTTTAGGATGCTTAAATTGTTCCGGTTTCGGTCTTAATTACACTGATATTGGTTTTTATGTTCCATTAAGAAAGGCTTTAAGGACTTGTCTTAATAGCATAATTGAGAGGAAAGTTGCTAAAGGGAATGGAATTGGTGTAATTGTGAAAACTTTTTCACCTTCTCATTTTGATGGTGATTGGGATAAGGCTGGAACATGTTCAAAGACTGAACCTTATGGAAAGGGTGAGAAGAAAATTGGAAAAATGGAAAGCGAGATTAGAAGGATTGAGATTGAAGAAGTGGAAAATGCTAAGAAAAAAGCAAATGAACTTGGAGGGATTAGATTTGAGGTATTGGATGTAACTAAATTGGCATTGTTGAGACCAGATGGTCATCCAGGTGCTTATATGAATCCTTTTCCGTTCGCTAACGGTGTTCCGAAGTATGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGGCCTATAGATACTTGGAATGAGATTTTTCTAGAGATGATGAAGAAGTGGGATTGGAAGGGACAGCCAAGGAGTGAAGAGTGA
Protein sequence

>Msa0806210

MKCTMKDDSISLSKTLLPYTLHLLLLIALLPIVLYLYFYPLSLTKSQTQLHHATISTPPHYSVNDKHKIDEHPCDYFNGKWIHDKKGPLYNGTTCGTIKQGQNCITNGRPDSNYLYYRWKPNECKLPRFEPNTFLQLSKNKHISFVGDSLARNQFESLLCMLATVSKPKPVHHKGSHWWHFSSHNATLSVYWSPFLVQGDERSKSGPNFNTIHLDRVNEKLAKDMDGIDLIVLSFGNWFINVPSVYYENGLVLGCLNCSGFGLNYTDIGFYVPLRKALRTCLNSIIERKVAKGNGIGVIVKTFSPSHFDGDWDKAGTCSKTEPYGKGEKKIGKMESEIRRIEIEEVENAKKKANELGGIRFEVLDVTKLALLRPDGHPGAYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEIFLEMMKKWDWKGQPRSEE*