AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0843530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843530 MtrunA17_Chr5g0414201 67.626 417 70 5 5 358 534 948 7.82e-175 512
Msa0843530 MtrunA17_Chr5g0414181 57.720 421 115 4 2 359 529 949 5.74e-145 436
Msa0843530 MtrunA17_Chr3g0087301 54.286 420 125 5 7 359 519 938 3.60e-130 400
Msa0843530 MtrunA17_Chr5g0414211 67.730 282 44 3 133 368 1 281 1.37e-117 348
Msa0843530 MtrunA17_Chr5g0414161 49.622 397 126 8 4 334 21 409 1.62e-105 317
Msa0843530 MtrunA17_Chr5g0415471 48.471 425 145 10 4 358 535 955 9.15e-100 320
Msa0843530 MtrunA17_Chr5g0414311 45.327 428 161 8 2 359 535 959 5.25e-99 318
Msa0843530 MtrunA17_Chr5g0414301 53.509 342 133 6 46 363 144 483 7.62e-97 300
Msa0843530 MtrunA17_Chr5g0417251 51.240 363 107 6 3 296 297 658 5.64e-95 297
Msa0843530 MtrunA17_Chr5g0415481 43.288 365 170 10 2 333 99 459 3.18e-69 225
Msa0843530 MtrunA17_Chr5g0413811 43.917 337 135 11 30 335 334 647 1.49e-62 212
Msa0843530 MtrunA17_Chr5g0414221 77.692 130 17 1 4 121 18 147 1.66e-59 189
Msa0843530 MtrunA17_Chr5g0430721 34.699 366 184 10 46 359 621 983 8.67e-45 166
Msa0843530 MtrunA17_Chr5g0430291 34.605 367 184 9 46 359 582 945 1.20e-44 165
Msa0843530 MtrunA17_Chr5g0430141 34.204 383 196 11 46 376 584 962 2.96e-44 164
Msa0843530 MtrunA17_Chr5g0430341 33.333 363 189 9 46 358 126 485 3.23e-44 160
Msa0843530 MtrunA17_Chr4g0027251 34.135 416 202 12 13 359 536 948 1.26e-43 162
Msa0843530 MtrunA17_Chr7g0244231 33.699 365 189 9 46 359 574 936 1.82e-43 162
Msa0843530 MtrunA17_Chr5g0429961 33.242 364 188 9 46 358 579 938 4.14e-43 160
Msa0843530 MtrunA17_Chr5g0430441 32.117 411 210 11 13 358 535 941 5.67e-43 160
Msa0843530 MtrunA17_Chr6g0470001 32.970 367 177 9 46 359 186 536 7.18e-43 159
Msa0843530 MtrunA17_Chr1g0155321 34.677 372 178 11 34 343 555 923 1.28e-42 159
Msa0843530 MtrunA17_Chr6g0470011 31.773 406 198 11 7 359 550 929 4.32e-42 158
Msa0843530 MtrunA17_Chr6g0470021 31.931 404 195 12 9 359 442 818 4.33e-42 157
Msa0843530 MtrunA17_Chr5g0430621 34.140 372 193 10 46 368 581 949 9.71e-42 157
Msa0843530 MtrunA17_Chr5g0430581 32.843 408 209 11 13 358 530 934 3.69e-41 155
Msa0843530 MtrunA17_Chr5g0431051 34.341 364 184 10 46 358 584 943 4.94e-41 154
Msa0843530 MtrunA17_Chr6g0470081 31.880 367 181 9 46 359 579 929 4.32e-40 152
Msa0843530 MtrunA17_Chr4g0071011 33.242 364 190 10 46 359 73 433 4.73e-40 149
Msa0843530 MtrunA17_Chr5g0430511 31.675 382 191 9 46 358 583 963 1.08e-38 147
Msa0843530 MtrunA17_Chr1g0155991 34.625 387 187 12 34 358 557 939 6.48e-38 145
Msa0843530 MtrunA17_Chr5g0430641 31.771 384 206 11 27 358 562 941 1.15e-36 142
Msa0843530 MtrunA17_Chr5g0429951 30.831 373 202 9 46 363 583 954 2.10e-36 141
Msa0843530 MtrunA17_Chr8g0351811 30.435 414 210 13 13 359 54 456 3.54e-33 131
Msa0843530 MtrunA17_Chr6g0467911 45.665 173 86 4 46 211 579 750 5.73e-32 127
Msa0843530 MtrunA17_Chr5g0430901 31.680 363 166 11 42 359 497 822 1.31e-30 124
Msa0843530 MtrunA17_Chr3g0085931 29.891 368 194 9 46 359 577 934 2.92e-30 123
Msa0843530 MtrunA17_Chr6g0468151 28.099 363 194 6 46 359 581 925 3.61e-30 122
Msa0843530 MtrunA17_Chr6g0468121 30.000 370 197 10 46 359 576 939 6.29e-30 122
Msa0843530 MtrunA17_Chr3g0123111 29.428 367 194 9 46 359 574 928 4.86e-29 119
Msa0843530 MtrunA17_Chr6g0468101 27.250 400 199 8 46 354 536 934 5.42e-29 119
Msa0843530 MtrunA17_Chr5g0430951 32.424 330 159 11 13 280 535 862 2.70e-27 114
Msa0843530 MtrunA17_Chr6g0468201 29.132 357 195 9 57 359 191 543 4.12e-27 113
Msa0843530 MtrunA17_Chr6g0467961 31.627 332 161 8 8 277 523 850 2.54e-26 111
Msa0843530 MtrunA17_Chr6g0468271 28.142 366 202 8 46 359 572 928 3.73e-26 110
Msa0843530 MtrunA17_Chr6g0468451 28.261 368 201 9 46 359 575 933 1.98e-25 108
Msa0843530 MtrunA17_Chr6g0468021 29.348 368 202 9 46 359 573 936 6.89e-24 104
Msa0843530 MtrunA17_Chr5g0410301 26.735 389 202 12 46 357 572 954 1.86e-22 99.8
Msa0843530 MtrunA17_Chr6g0468361 31.174 247 120 5 46 242 184 430 4.29e-22 97.8
Msa0843530 MtrunA17_Chr3g0089651 30.488 328 184 15 46 357 607 906 2.57e-21 96.7
Msa0843530 MtrunA17_Chr2g0282991 39.247 186 100 7 46 222 599 780 3.00e-21 96.3
Msa0843530 MtrunA17_Chr3g0089251 30.581 327 185 15 46 357 632 931 4.93e-21 95.5
Msa0843530 MtrunA17_Chr3g0089211 29.969 327 198 13 46 351 650 966 6.33e-21 95.1
Msa0843530 MtrunA17_Chr3g0089761 31.212 330 178 16 46 357 603 901 7.66e-21 95.1
Msa0843530 MtrunA17_Chr3g0089781 30.088 339 205 14 46 362 603 931 2.29e-20 93.6
Msa0843530 MtrunA17_Chr3g0089811 31.707 328 180 16 46 357 603 902 4.42e-20 92.8
Msa0843530 MtrunA17_Chr5g0410161 30.000 280 123 10 46 268 717 980 8.46e-20 92.0
Msa0843530 MtrunA17_Chr3g0087701 29.912 341 179 14 46 357 593 902 1.27e-19 91.3
Msa0843530 MtrunA17_Chr3g0089421 29.664 327 188 15 46 357 605 904 1.80e-19 90.9
Msa0843530 MtrunA17_Chr3g0092241 31.003 329 187 16 46 360 603 905 6.21e-19 89.4
Msa0843530 MtrunA17_Chr3g0089601 30.183 328 185 15 46 357 601 900 7.06e-19 89.0
Msa0843530 MtrunA17_Chr2g0309721 29.834 362 200 16 23 357 565 899 1.30e-18 88.2
Msa0843530 MtrunA17_Chr4g0025391 30.321 343 179 16 46 352 601 919 1.46e-18 88.2
Msa0843530 MtrunA17_Chr3g0089471 30.793 328 183 15 46 357 651 950 1.59e-18 87.8
Msa0843530 MtrunA17_Chr3g0086221 30.588 340 170 18 46 357 588 889 2.32e-18 87.4
Msa0843530 MtrunA17_Chr3g0089441 28.286 350 197 13 46 362 109 437 3.22e-18 86.7
Msa0843530 MtrunA17_Chr3g0090691 25.000 400 206 14 46 357 604 997 3.32e-18 87.0
Msa0843530 MtrunA17_Chr3g0085751 29.738 343 181 14 46 357 589 902 3.48e-18 87.0
Msa0843530 MtrunA17_Chr3g0089501 35.106 188 108 7 46 225 601 782 3.91e-18 86.7
Msa0843530 MtrunA17_Chr3g0089541 29.573 328 187 15 46 357 606 905 3.96e-18 86.7
Msa0843530 MtrunA17_Chr5g0446611 35.870 184 106 6 47 222 604 783 3.97e-18 86.7
Msa0843530 MtrunA17_Chr3g0086621 28.824 340 184 14 46 357 589 898 5.51e-18 86.3
Msa0843530 MtrunA17_Chr5g0410401 27.320 388 182 18 46 359 446 807 7.46e-18 85.9
Msa0843530 MtrunA17_Chr5g0410481 26.044 407 214 13 46 368 560 963 1.10e-17 85.5
Msa0843530 MtrunA17_Chr5g0410351 25.610 410 215 14 46 368 570 976 1.12e-17 85.5
Msa0843530 MtrunA17_Chr3g0082131 28.117 377 191 16 46 358 611 971 1.30e-17 85.1
Msa0843530 MtrunA17_Chr3g0086161 28.448 348 177 16 46 357 589 900 1.34e-17 85.1
Msa0843530 MtrunA17_Chr3g0086151 31.602 231 132 10 46 268 589 801 1.48e-17 84.7
Msa0843530 MtrunA17_Chr1g0164581 28.117 377 191 16 46 358 587 947 1.55e-17 85.1
Msa0843530 MtrunA17_Chr3g0085711 29.825 342 182 15 46 357 161 474 2.34e-17 84.0
Msa0843530 MtrunA17_Chr3g0092261 30.606 330 187 16 46 360 647 949 2.70e-17 84.3
Msa0843530 MtrunA17_Chr3g0086091 34.682 173 104 5 46 211 400 570 3.00e-17 84.0
Msa0843530 MtrunA17_Chr3g0090651 27.103 321 168 11 46 303 604 921 3.37e-17 84.0
Msa0843530 MtrunA17_Chr7g0255751 38.926 149 82 4 46 185 62 210 5.26e-17 79.7
Msa0843530 MtrunA17_Chr3g0086071 28.395 324 171 13 46 361 209 479 5.73e-17 82.8
Msa0843530 MtrunA17_Chr3g0089991 30.605 281 146 12 27 264 560 834 7.76e-17 82.8
Msa0843530 MtrunA17_Chr7g0248531 29.674 337 180 14 39 364 583 873 1.00e-16 82.4
Msa0843530 MtrunA17_Chr3g0091061 25.000 400 207 14 46 357 604 998 1.08e-16 82.4
Msa0843530 MtrunA17_Chr3g0085651 28.367 349 182 16 46 361 599 912 1.12e-16 82.4
Msa0843530 MtrunA17_Chr3g0084971 30.128 312 158 11 46 300 594 902 1.17e-16 82.4
Msa0843530 MtrunA17_Chr5g0410261 30.935 278 150 11 34 270 577 853 1.22e-16 82.0
Msa0843530 MtrunA17_Chr3g0086181 34.503 171 99 6 46 209 589 753 1.24e-16 82.0
Msa0843530 MtrunA17_Chr3g0085551 35.838 173 100 6 46 209 481 651 2.59e-16 80.9
Msa0843530 MtrunA17_Chr3g0091011 28.863 343 184 14 46 357 573 886 2.79e-16 80.9
Msa0843530 MtrunA17_Chr3g0085091 34.682 173 102 6 46 209 597 767 3.68e-16 80.9
Msa0843530 MtrunA17_Chr8g0356251 28.117 377 196 17 46 358 627 992 3.95e-16 80.5
Msa0843530 MtrunA17_Chr3g0089871 34.286 210 121 9 46 245 606 808 4.07e-16 80.5
Msa0843530 MtrunA17_Chr3g0082391 36.095 169 93 7 46 204 606 769 4.16e-16 80.5
Msa0843530 MtrunA17_Chr3g0087841 27.301 326 196 14 46 357 603 901 5.57e-16 80.1
Msa0843530 MtrunA17_Chr3g0091081 27.103 321 167 12 46 303 602 918 6.82e-16 79.7
Msa0843530 MtrunA17_Chr3g0090431 31.383 188 115 7 46 225 475 656 7.30e-16 79.7
Msa0843530 MtrunA17_Chr3g0085131 28.440 327 163 16 46 361 591 857 1.66e-15 78.6
Msa0843530 MtrunA17_Chr3g0082311 36.527 167 93 7 46 204 605 766 2.03e-15 78.6
Msa0843530 MtrunA17_Chr3g0090371 31.053 190 113 7 46 225 602 783 2.86e-15 77.8
Msa0843530 MtrunA17_Chr3g0085171 34.682 173 102 6 46 209 581 751 3.16e-15 77.8
Msa0843530 MtrunA17_Chr3g0083751 38.012 171 90 9 46 204 606 772 3.35e-15 77.8
Msa0843530 MtrunA17_Chr3g0083621 35.119 168 99 6 46 205 612 777 4.24e-15 77.4
Msa0843530 MtrunA17_Chr7g0221481 35.838 173 98 7 46 209 600 768 4.43e-15 77.4
Msa0843530 MtrunA17_Chr3g0085521 34.161 161 96 5 46 197 586 745 4.65e-15 77.4
Msa0843530 MtrunA17_Chr8g0352831 33.533 167 99 5 46 204 620 782 5.50e-15 77.0
Msa0843530 MtrunA17_Chr3g0085801 31.950 241 133 11 46 273 571 793 5.53e-15 77.0
Msa0843530 MtrunA17_Chr3g0089961 26.300 327 185 10 46 361 606 887 5.62e-15 77.0
Msa0843530 MtrunA17_Chr3g0083641 37.427 171 91 8 46 204 577 743 6.76e-15 76.6
Msa0843530 MtrunA17_Chr3g0086741 34.682 173 102 6 46 209 618 788 7.69e-15 76.6
Msa0843530 MtrunA17_Chr3g0084051 32.479 234 126 12 46 268 605 817 8.96e-15 76.3
Msa0843530 MtrunA17_Chr1g0164391 34.857 175 96 7 46 204 606 778 9.81e-15 76.3
Msa0843530 MtrunA17_Chr5g0415551 36.585 164 97 6 46 204 461 622 1.04e-14 75.9
Msa0843530 MtrunA17_Chr6g0484551 34.884 172 100 6 46 209 577 744 1.11e-14 75.9
Msa0843530 MtrunA17_Chr3g0082351 37.356 174 94 8 46 209 603 771 1.41e-14 75.9
Msa0843530 MtrunA17_Chr3g0083721 35.928 167 97 6 46 204 544 708 1.97e-14 75.1
Msa0843530 MtrunA17_Chr3g0091121 26.500 400 200 18 46 357 214 607 3.71e-14 74.3
Msa0843530 MtrunA17_Chr3g0085111 26.524 328 174 12 46 361 592 864 3.74e-14 74.3
Msa0843530 MtrunA17_Chr8g0392511 33.140 172 103 6 46 209 164 331 3.86e-14 73.2
Msa0843530 MtrunA17_Chr3g0087541 27.859 341 154 17 46 358 604 880 4.42e-14 74.3
Msa0843530 MtrunA17_Chr3g0085561 27.078 373 210 14 46 361 584 951 4.52e-14 74.3
Msa0843530 MtrunA17_Chr3g0082811 33.714 175 101 6 46 209 586 756 5.53e-14 73.9
Msa0843530 MtrunA17_Chr3g0089591 28.075 374 180 16 46 357 607 953 5.72e-14 73.9
Msa0843530 MtrunA17_Chr3g0090141 31.818 154 94 5 46 191 603 753 7.62e-14 73.6
Msa0843530 MtrunA17_Chr3g0085161 27.907 344 186 15 46 357 455 768 1.01e-13 73.2
Msa0843530 MtrunA17_Chr3g0082321 35.329 167 95 7 46 204 551 712 1.04e-13 72.8
Msa0843530 MtrunA17_Chr1g0147141 26.617 402 196 18 46 358 605 996 1.16e-13 72.8
Msa0843530 MtrunA17_Chr3g0087581 33.333 171 105 5 46 209 590 758 1.51e-13 72.8
Msa0843530 MtrunA17_Chr8g0348931 31.651 218 134 8 46 254 609 820 1.63e-13 72.4
Msa0843530 MtrunA17_Chr1g0164611 34.043 188 102 9 46 215 615 798 1.97e-13 72.4
Msa0843530 MtrunA17_Chr3g0082101 34.043 188 102 9 46 215 563 746 2.19e-13 72.0
Msa0843530 MtrunA17_Chr3g0083681 35.329 167 98 6 46 204 578 742 4.40e-13 71.2
Msa0843530 MtrunA17_Chr3g0083591 34.568 162 96 6 51 204 571 730 6.77e-13 70.5
Msa0843530 MtrunA17_Chr4g0010201 25.735 408 204 15 46 359 597 999 7.70e-13 70.5
Msa0843530 MtrunA17_Chr2g0299151 25.697 323 188 11 46 361 594 871 8.09e-13 70.5
Msa0843530 MtrunA17_Chr6g0467921 32.258 155 90 4 209 359 1 144 8.62e-13 68.9
Msa0843530 MtrunA17_Chr4g0009781 24.069 403 215 13 46 359 598 998 9.03e-13 70.1
Msa0843530 MtrunA17_Chr3g0085331 30.058 173 109 6 46 211 615 782 9.54e-13 70.1
Msa0843530 MtrunA17_Chr6g0468341 27.402 281 138 9 129 359 472 736 1.35e-12 69.7
Msa0843530 MtrunA17_Chr3g0084031 33.714 175 102 7 46 209 607 778 2.39e-12 68.9
Msa0843530 MtrunA17_Chr4g0020501 25.000 368 191 13 58 358 488 837 5.35e-12 67.8
Msa0843530 MtrunA17_Chr3g0082791 28.267 375 211 18 46 390 606 952 9.86e-12 67.0
Msa0843530 MtrunA17_Chr4g0010221 33.149 181 107 7 46 212 596 776 1.29e-11 66.6
Msa0843530 MtrunA17_Chr2g0316291 26.024 415 198 17 46 357 575 983 1.51e-11 66.2
Msa0843530 MtrunA17_Chr4g0010261 34.932 146 84 5 46 180 598 743 3.20e-11 65.5
Msa0843530 MtrunA17_Chr4g0010251 27.811 338 212 14 46 362 593 919 4.68e-11 64.7
Msa0843530 MtrunA17_Chr3g0085471 26.609 233 129 9 46 265 605 808 5.13e-11 64.7
Msa0843530 MtrunA17_Chr3g0080871 32.967 182 110 7 51 224 594 771 8.46e-11 63.9
Msa0843530 MtrunA17_Chr3g0085501 29.762 168 105 6 46 207 615 775 8.58e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843530 AT3G14470.1 26.546 388 212 12 43 358 602 988 6.42e-18 86.7
Msa0843530 AT3G14470.2 26.546 388 212 12 43 358 602 988 6.42e-18 86.7
Msa0843530 AT3G14460.1 39.394 132 72 4 46 170 599 729 2.62e-13 72.4

Find 82 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATAATTCTAATAAAAT+AGG 0.122851 5_4:+21173344 None:intergenic
CCTTCCATCTCTCATTGATA+TGG 0.210039 5_4:-21175683 Msa0843530:CDS
AGGCTTAAGTGTATTCTCTT+AGG 0.224893 5_4:-21173467 Msa0843530:exon
AGGTACTTGAATCTTTCTTA+TGG 0.246045 5_4:-21176510 Msa0843530:CDS
AACCTCCTGCAACTCCATTA+TGG 0.246508 5_4:+21175842 None:intergenic
AAAGGCAAGCCCAACAATTT+AGG 0.253032 5_4:+21175706 None:intergenic
CCAGACTATTAGGCAACTTT+TGG 0.258593 5_4:+21176409 None:intergenic
GGTGTTAATGTTAAAGATTT+CGG 0.295929 5_4:+21175480 None:intergenic
GGATGGGAATTGCTCAAGTT+TGG 0.326605 5_4:+21175501 None:intergenic
CATCCCTTGATAAGGCTATC+AGG 0.335767 5_4:+21175779 None:intergenic
CTTCGAAGTTGCTGAGTTTG+AGG 0.337667 5_4:+21176060 None:intergenic
TTCAAGTGTACCAGACTATT+AGG 0.343140 5_4:+21176399 None:intergenic
CACAACGTACATAAGGATTC+TGG 0.350495 5_4:+21176471 None:intergenic
ACCTCCTGCAACTCCATTAT+GGG 0.351088 5_4:+21175843 None:intergenic
CTTCATTGTGGTTGAACATA+AGG 0.357595 5_4:+21175596 None:intergenic
TACTGAATGTCCTAAATTGT+TGG 0.361413 5_4:-21175716 Msa0843530:CDS
TTCTCTATCATCCCTTGATA+AGG 0.370077 5_4:+21175771 None:intergenic
CTTCCATCTCTCATTGATAT+GGG 0.376582 5_4:-21175682 Msa0843530:intron
TCGTCCCATAATGGAGTTGC+AGG 0.387609 5_4:-21175847 Msa0843530:CDS
ATAATAATTCTAATAAAATA+GGG 0.394282 5_4:+21173345 None:intergenic
AAATACTTTGCATTAAGAAT+AGG 0.399908 5_4:+21173266 None:intergenic
ACTGAATGTCCTAAATTGTT+GGG 0.400442 5_4:-21175715 Msa0843530:CDS
GGCTTAAGTGTATTCTCTTA+GGG 0.414020 5_4:-21173466 Msa0843530:exon
CCAAAAGTTGCCTAATAGTC+TGG 0.414031 5_4:-21176409 Msa0843530:CDS
TTGCAGGTGGAACATGTTAG+AGG 0.415349 5_4:-21176694 None:intergenic
CTTGATAAGGCTATCAGGTT+TGG 0.421804 5_4:+21175784 None:intergenic
GACGAGGAGTCGTCCCATAA+TGG 0.428211 5_4:-21175856 Msa0843530:CDS
TTCATTGTGGTTGAACATAA+GGG 0.431182 5_4:+21175597 None:intergenic
TAATGTAAAGGTCTCCTTGA+AGG 0.444130 5_4:+21176229 None:intergenic
GCTTGACATATTAGCTTCCT+TGG 0.444359 5_4:+21176173 None:intergenic
CAGTAATTTGAAATTTAGAA+AGG 0.444658 5_4:+21175734 None:intergenic
CAAATTCCACAACGTACATA+AGG 0.470935 5_4:+21176464 None:intergenic
AGGTTTCTTAGCATCCCATC+TGG 0.475533 5_4:+21175559 None:intergenic
TTTGATATATATTTCTTGGA+TGG 0.485821 5_4:+21175459 None:intergenic
TCTCCAAGTGCTTAATGTAA+AGG 0.491947 5_4:+21176217 None:intergenic
AGGAATAACATCTCAATACA+TGG 0.492791 5_4:+21176598 None:intergenic
CAAACCTGATAGCCTTATCA+AGG 0.493624 5_4:-21175783 Msa0843530:CDS
AAATGAAGTGTCACAAGTAC+AGG 0.494355 5_4:-21176115 Msa0843530:CDS
ATGCCAACATTCTTACATGC+GGG 0.494866 5_4:+21176325 None:intergenic
AGCTAACTTGCTTTCCAGAT+GGG 0.497385 5_4:-21175573 Msa0843530:CDS
AGACCTTTACATTAAGCACT+TGG 0.498417 5_4:-21176220 Msa0843530:CDS
TAAACAGTATGAAAATATCT+TGG 0.501346 5_4:-21173432 Msa0843530:three_prime_UTR
TGGAACATGTTAGAGGAAGT+TGG 0.514806 5_4:-21176687 None:intergenic
AGATCAAATTCTGTTGCATC+CGG 0.525216 5_4:-21173532 Msa0843530:CDS
TTAGGACAAATGAACCTTCA+AGG 0.526890 5_4:-21176243 Msa0843530:CDS
GACATCCTCTTGTTCAAGAC+CGG 0.527277 5_4:+21173513 None:intergenic
TTAATGTTAAAGATTTCGGA+TGG 0.530887 5_4:+21175484 None:intergenic
CTCGAAGTCAAGCACTCGTA+AGG 0.536802 5_4:+21176578 None:intergenic
CTTCGAAGTCTGTATGTTGG+AGG 0.538768 5_4:-21176045 Msa0843530:CDS
GGACAATTTGAAATTGTCAT+GGG 0.548122 5_4:-21176142 Msa0843530:CDS
GAGCTAACTTGCTTTCCAGA+TGG 0.548886 5_4:-21175574 Msa0843530:CDS
CAGAATCCTTATGTACGTTG+TGG 0.551748 5_4:-21176470 Msa0843530:CDS
TCAATACATGGGGTGAGAGT+TGG 0.554333 5_4:+21176610 None:intergenic
AGATGGGATGCTAAGAAACC+TGG 0.563751 5_4:-21175557 Msa0843530:CDS
ATTTGAAATTTAGAAAGGTG+TGG 0.566416 5_4:+21175739 None:intergenic
TGGACAATTTGAAATTGTCA+TGG 0.569957 5_4:-21176143 Msa0843530:CDS
GGAATAACATCTCAATACAT+GGG 0.574024 5_4:+21176599 None:intergenic
TCAATGAGAGATGGAAGGAA+AGG 0.574345 5_4:+21175688 None:intergenic
TCAATAATAACTAAGAAATG+TGG 0.577722 5_4:+21173379 None:intergenic
CAACTTCGAAGTCTGTATGT+TGG 0.580363 5_4:-21176048 Msa0843530:CDS
CATTAAGAATAGGTTTGACA+CGG 0.582049 5_4:+21173276 None:intergenic
TAATGTTAAAGATTTCGGAT+GGG 0.586146 5_4:+21175485 None:intergenic
ATGGGTAATCAGAGTGGACA+AGG 0.589821 5_4:-21173561 Msa0843530:CDS
TGCATCCGGTCTTGAACAAG+AGG 0.595969 5_4:-21173518 Msa0843530:CDS
ATACCCATATCAATGAGAGA+TGG 0.600725 5_4:+21175679 None:intergenic
AGCAAGTTAGCTCTTCATTG+TGG 0.608038 5_4:+21175584 None:intergenic
AGTAAAATGGGTAATCAGAG+TGG 0.614238 5_4:-21173567 Msa0843530:CDS
TAAAAGTGTAATGAATGCCA+AGG 0.617269 5_4:-21176190 Msa0843530:CDS
TGCCAACATTCTTACATGCG+GGG 0.617367 5_4:+21176326 None:intergenic
TCCCATAATGGAGTTGCAGG+AGG 0.618291 5_4:-21175844 Msa0843530:CDS
ACAAGTACAGGAAAATGTTG+AGG 0.624923 5_4:-21176103 Msa0843530:CDS
CCATATCAATGAGAGATGGA+AGG 0.625295 5_4:+21175683 None:intergenic
TTAAGTGTATTCTCTTAGGG+AGG 0.626896 5_4:-21173463 Msa0843530:exon
GCCAACATTCTTACATGCGG+GGG 0.632733 5_4:+21176327 None:intergenic
CTGTATGTTGGAGGATACAC+CGG 0.637156 5_4:-21176036 Msa0843530:intron
AAACCTGATAGCCTTATCAA+GGG 0.638408 5_4:-21175782 Msa0843530:CDS
ATAAAAGGAAAATGCAACAC+GGG 0.645734 5_4:-21175658 Msa0843530:intron
TAATATGTCAAGCAAGTGCG+TGG 0.652604 5_4:-21176163 Msa0843530:CDS
GAATAACATCTCAATACATG+GGG 0.663735 5_4:+21176600 None:intergenic
GATGCCAACATTCTTACATG+CGG 0.676201 5_4:+21176324 None:intergenic
TATAAAAGGAAAATGCAACA+CGG 0.720090 5_4:-21175659 Msa0843530:intron
TCATGTAAAATACTTAGACG+AGG 0.738566 5_4:-21175872 Msa0843530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTGATATATATTTCT+TGG + chr5_4:21174483-21174502 None:intergenic 10.0%
!!! TTTTCAAATTAAAGTTTTTT+AGG - chr5_4:21176100-21176119 Msa0843530:CDS 10.0%
!! CATAATAATTCTAATAAAAT+AGG + chr5_4:21176594-21176613 None:intergenic 10.0%
!!! ATATAGTGTTAAAATTTTCA+AGG + chr5_4:21173636-21173655 None:intergenic 15.0%
!!! TTTTTCATTTTGTTGAATTT+TGG - chr5_4:21175116-21175135 Msa0843530:intron 15.0%
!!! TTTGAAATTTGAAATTTTAG+TGG - chr5_4:21175441-21175460 Msa0843530:intron 15.0%
!!! TGTGATTTATTTTTAATTGT+TGG - chr5_4:21175758-21175777 Msa0843530:CDS 15.0%
!! AATGTTGAATGAAATATATT+TGG - chr5_4:21175987-21176006 Msa0843530:intron 15.0%
!! TAAATGTAATACAAAACATT+AGG + chr5_4:21176327-21176346 None:intergenic 15.0%
!!! ATTTTAACACTATATGTAGT+TGG - chr5_4:21173641-21173660 Msa0843530:intron 20.0%
!!! CTAGTTAAATATTTTAGAGT+AGG + chr5_4:21173953-21173972 None:intergenic 20.0%
!!! CAGTAATTTGAAATTTAGAA+AGG + chr5_4:21174204-21174223 None:intergenic 20.0%
!! TAAAAACATTTGACATTTGT+GGG - chr5_4:21174407-21174426 Msa0843530:intron 20.0%
!! TTTGATATATATTTCTTGGA+TGG + chr5_4:21174479-21174498 None:intergenic 20.0%
!! AAATTTGTAATGCTTCAAAA+GGG + chr5_4:21175064-21175083 None:intergenic 20.0%
!! AAAATTTGTAATGCTTCAAA+AGG + chr5_4:21175065-21175084 None:intergenic 20.0%
!!! TTTTGAAGCATTACAAATTT+TGG - chr5_4:21175064-21175083 Msa0843530:intron 20.0%
!!! TTTGAAGCATTACAAATTTT+GGG - chr5_4:21175065-21175084 Msa0843530:intron 20.0%
!!! AAACTGAATGATACAAATTT+TGG - chr5_4:21175193-21175212 Msa0843530:intron 20.0%
!!! AACTGAATGATACAAATTTT+GGG - chr5_4:21175194-21175213 Msa0843530:intron 20.0%
!!! TGTACTTTTTGTTTGTTTTA+TGG + chr5_4:21175603-21175622 None:intergenic 20.0%
!! ATACACTGAAAATAACTTAT+TGG + chr5_4:21175884-21175903 None:intergenic 20.0%
!! AAATATATTTGGAGAAAGTA+GGG - chr5_4:21175998-21176017 Msa0843530:intron 20.0%
!! GTGAAATGATATATAACAAT+TGG + chr5_4:21176279-21176298 None:intergenic 20.0%
!! TAAACAGTATGAAAATATCT+TGG - chr5_4:21176503-21176522 Msa0843530:CDS 20.0%
!! TCAATAATAACTAAGAAATG+TGG + chr5_4:21176559-21176578 None:intergenic 20.0%
!! AAATACTTTGCATTAAGAAT+AGG + chr5_4:21176672-21176691 None:intergenic 20.0%
!! TTGGTCGTTTAAAATATCTA+AGG - chr5_4:21173405-21173424 Msa0843530:three_prime_UTR 25.0%
!! ATTTGAAATTTAGAAAGGTG+TGG + chr5_4:21174199-21174218 None:intergenic 25.0%
! TATAAAAGGAAAATGCAACA+CGG - chr5_4:21174276-21174295 Msa0843530:intron 25.0%
! CTAAAAACATTTGACATTTG+TGG - chr5_4:21174406-21174425 Msa0843530:intron 25.0%
! TAATGTTAAAGATTTCGGAT+GGG + chr5_4:21174453-21174472 None:intergenic 25.0%
! TTAATGTTAAAGATTTCGGA+TGG + chr5_4:21174454-21174473 None:intergenic 25.0%
! GGTGTTAATGTTAAAGATTT+CGG + chr5_4:21174458-21174477 None:intergenic 25.0%
! GTAATTCTTGAAGTAAACTT+AGG + chr5_4:21174690-21174709 None:intergenic 25.0%
! CTTAAAAGTTTGAGAATTTG+TGG - chr5_4:21174769-21174788 Msa0843530:intron 25.0%
!!! GGTATTTTAATAGAAAACAG+AGG - chr5_4:21174862-21174881 Msa0843530:intron 25.0%
!!! ACTAGTATAATTTTGAAGTG+AGG - chr5_4:21175030-21175049 Msa0843530:intron 25.0%
!!! CTAGTATAATTTTGAAGTGA+GGG - chr5_4:21175031-21175050 Msa0843530:intron 25.0%
! TATCATCTACTCAATATTCT+TGG - chr5_4:21175343-21175362 Msa0843530:intron 25.0%
! AAATAACAAGCTTGAAATTC+TGG + chr5_4:21175523-21175542 None:intergenic 25.0%
! GAAATATATTTGGAGAAAGT+AGG - chr5_4:21175997-21176016 Msa0843530:intron 25.0%
!! CAAGTTACTTTCTTGTAAAA+TGG + chr5_4:21176066-21176085 None:intergenic 25.0%
!!! ACATTTTACTTTTTTTCCCT+GGG + chr5_4:21176174-21176193 None:intergenic 25.0%
!! CATTTATGACAATTCTGTTT+TGG - chr5_4:21176341-21176360 Msa0843530:CDS 25.0%
! GAATGAATAAAAAGCAATGT+AGG + chr5_4:21176447-21176466 None:intergenic 25.0%
!!! CATTGCTTTTTATTCATTCA+AGG - chr5_4:21176448-21176467 Msa0843530:CDS 25.0%
!! TTTTCATACTGTTTAACACT+AGG + chr5_4:21176498-21176517 None:intergenic 25.0%
GAATAACATCTCAATACATG+GGG + chr5_4:21173338-21173357 None:intergenic 30.0%
GGAATAACATCTCAATACAT+GGG + chr5_4:21173339-21173358 None:intergenic 30.0%
AGGAATAACATCTCAATACA+TGG + chr5_4:21173340-21173359 None:intergenic 30.0%
AGGTACTTGAATCTTTCTTA+TGG - chr5_4:21173425-21173444 Msa0843530:three_prime_UTR 30.0%
TATGTAGTTGGCATGAAAAA+AGG - chr5_4:21173653-21173672 Msa0843530:intron 30.0%
! TAAAAGTGTAATGAATGCCA+AGG - chr5_4:21173745-21173764 Msa0843530:intron 30.0%
! TGGACAATTTGAAATTGTCA+TGG - chr5_4:21173792-21173811 Msa0843530:intron 30.0%
! GGACAATTTGAAATTGTCAT+GGG - chr5_4:21173793-21173812 Msa0843530:intron 30.0%
TCATGTAAAATACTTAGACG+AGG - chr5_4:21174063-21174082 Msa0843530:intron 30.0%
TACTGAATGTCCTAAATTGT+TGG - chr5_4:21174219-21174238 Msa0843530:intron 30.0%
ACTGAATGTCCTAAATTGTT+GGG - chr5_4:21174220-21174239 Msa0843530:intron 30.0%
CTCATTGATATGGGTATAAA+AGG - chr5_4:21174262-21174281 Msa0843530:intron 30.0%
ATAAAAGGAAAATGCAACAC+GGG - chr5_4:21174277-21174296 Msa0843530:intron 30.0%
TTCATTGTGGTTGAACATAA+GGG + chr5_4:21174341-21174360 None:intergenic 30.0%
! TTGACAGATGAAGTTTTACA+AGG - chr5_4:21174520-21174539 Msa0843530:intron 30.0%
! TGACAGATGAAGTTTTACAA+GGG - chr5_4:21174521-21174540 Msa0843530:intron 30.0%
!!! TCAAACTTTTAAGACCAGTA+AGG + chr5_4:21174762-21174781 None:intergenic 30.0%
!!! AGCATTACAAATTTTGGGTT+TGG - chr5_4:21175070-21175089 Msa0843530:intron 30.0%
!!! ATTACAAATTTTGGGTTTGG+TGG - chr5_4:21175073-21175092 Msa0843530:intron 30.0%
!!! TTACAAATTTTGGGTTTGGT+GGG - chr5_4:21175074-21175093 Msa0843530:intron 30.0%
!!! TTGGGTTTTATTTCACTACT+TGG - chr5_4:21175212-21175231 Msa0843530:intron 30.0%
AATAGCTAGAAGAAAATGAC+AGG + chr5_4:21175579-21175598 None:intergenic 30.0%
TCTCAGATGATTAATCATAG+TGG - chr5_4:21175652-21175671 Msa0843530:CDS 30.0%
CTCAGATGATTAATCATAGT+GGG - chr5_4:21175653-21175672 Msa0843530:CDS 30.0%
TTTAATTGTTGGCGTCAAAA+TGG - chr5_4:21175769-21175788 Msa0843530:CDS 30.0%
ACTTATTGGATACTACTGAT+TGG + chr5_4:21175870-21175889 None:intergenic 30.0%
TTGAGAATAGATTTAGCTGT+GGG - chr5_4:21175947-21175966 Msa0843530:intron 30.0%
!!! ATTTTACTTTTTTTCCCTGG+GGG + chr5_4:21176172-21176191 None:intergenic 30.0%
!!! CATTTTACTTTTTTTCCCTG+GGG + chr5_4:21176173-21176192 None:intergenic 30.0%
!!! CACATTTTACTTTTTTTCCC+TGG + chr5_4:21176175-21176194 None:intergenic 30.0%
AATGCACAAGATAGTTGAAT+GGG + chr5_4:21176242-21176261 None:intergenic 30.0%
CATTAAGAATAGGTTTGACA+CGG + chr5_4:21176662-21176681 None:intergenic 30.0%
GAAAAGTTGTCGTCTTCAAT+TGG - chr5_4:21173386-21173405 Msa0843530:three_prime_UTR 35.0%
CAAATTCCACAACGTACATA+AGG + chr5_4:21173474-21173493 None:intergenic 35.0%
TTCAAGTGTACCAGACTATT+AGG + chr5_4:21173539-21173558 None:intergenic 35.0%
! GGTTTTCTTTTAGCAGAGTT+AGG - chr5_4:21173674-21173693 Msa0843530:intron 35.0%
TTAGGACAAATGAACCTTCA+AGG - chr5_4:21173692-21173711 Msa0843530:intron 35.0%
TAATGTAAAGGTCTCCTTGA+AGG + chr5_4:21173709-21173728 None:intergenic 35.0%
AGACCTTTACATTAAGCACT+TGG - chr5_4:21173715-21173734 Msa0843530:intron 35.0%
TCTCCAAGTGCTTAATGTAA+AGG + chr5_4:21173721-21173740 None:intergenic 35.0%
! AAATGAAGTGTCACAAGTAC+AGG - chr5_4:21173820-21173839 Msa0843530:intron 35.0%
ACAAGTACAGGAAAATGTTG+AGG - chr5_4:21173832-21173851 Msa0843530:intron 35.0%
TTGTTTACACCTTCCACATT+TGG - chr5_4:21173997-21174016 Msa0843530:intron 35.0%
TGTTTACACCTTCCACATTT+GGG - chr5_4:21173998-21174017 Msa0843530:intron 35.0%
! ACTGCTAAACTCTTTAGAGA+TGG + chr5_4:21174031-21174050 None:intergenic 35.0%
!! AAACCTGATAGCCTTATCAA+GGG - chr5_4:21174153-21174172 Msa0843530:intron 35.0%
TTCTCTATCATCCCTTGATA+AGG + chr5_4:21174167-21174186 None:intergenic 35.0%
CTTCCATCTCTCATTGATAT+GGG - chr5_4:21174253-21174272 Msa0843530:intron 35.0%
ATACCCATATCAATGAGAGA+TGG + chr5_4:21174259-21174278 None:intergenic 35.0%
CTTCATTGTGGTTGAACATA+AGG + chr5_4:21174342-21174361 None:intergenic 35.0%
!!! CAAATGTTTTTAGAGAAGCC+AGG + chr5_4:21174399-21174418 None:intergenic 35.0%
TTAGTCATTGAATCTTGCTC+AGG - chr5_4:21174562-21174581 Msa0843530:intron 35.0%
AAGTCATATGTTGTAAAGCC+GGG + chr5_4:21174603-21174622 None:intergenic 35.0%
GAAGTCATATGTTGTAAAGC+CGG + chr5_4:21174604-21174623 None:intergenic 35.0%
TATGACTTCCCTTCAATCAT+TGG - chr5_4:21174615-21174634 Msa0843530:intron 35.0%
ACTCAATGCCAATGATTGAA+GGG + chr5_4:21174626-21174645 None:intergenic 35.0%
!! CCTTGAACATGAGCTATTTT+TGG + chr5_4:21174844-21174863 None:intergenic 35.0%
CCAAAAATAGCTCATGTTCA+AGG - chr5_4:21174841-21174860 Msa0843530:intron 35.0%
TATTATACTCACGTGAGAAG+TGG - chr5_4:21174999-21175018 Msa0843530:intron 35.0%
!!! GTATAATTTTGAAGTGAGGG+TGG - chr5_4:21175034-21175053 Msa0843530:intron 35.0%
!! GGTGGGTGTCATTTAAATTT+AGG - chr5_4:21175091-21175110 Msa0843530:intron 35.0%
!!! TCCTGTGTTTTTTTTTCCAC+AGG - chr5_4:21175145-21175164 Msa0843530:intron 35.0%
ACCTGTGGAAAAAAAAACAC+AGG + chr5_4:21175149-21175168 None:intergenic 35.0%
!!! TGTTTTTTTTTCCACAGGTC+TGG - chr5_4:21175150-21175169 Msa0843530:intron 35.0%
! AACCCAGTTGTATATTTTCC+CGG + chr5_4:21175262-21175281 None:intergenic 35.0%
AGGCGATATAATGCTACAAT+TGG + chr5_4:21175301-21175320 None:intergenic 35.0%
!! TAATCATAGTGGGTACTAGT+TGG - chr5_4:21175663-21175682 Msa0843530:intron 35.0%
! TAGAGGCAGATGAACTTTTT+GGG + chr5_4:21175703-21175722 None:intergenic 35.0%
! ATAGAGGCAGATGAACTTTT+TGG + chr5_4:21175704-21175723 None:intergenic 35.0%
GCATGAACATTAACGAATAG+AGG + chr5_4:21175720-21175739 None:intergenic 35.0%
CTTGAGAATAGATTTAGCTG+TGG - chr5_4:21175946-21175965 Msa0843530:intron 35.0%
!!! TTTTACTTTTTTTCCCTGGG+GGG + chr5_4:21176171-21176190 None:intergenic 35.0%
TTGAATGGGGTATATTGACA+GGG + chr5_4:21176228-21176247 None:intergenic 35.0%
ATGCACAAGATAGTTGAATG+GGG + chr5_4:21176241-21176260 None:intergenic 35.0%
CAATGCACAAGATAGTTGAA+TGG + chr5_4:21176243-21176262 None:intergenic 35.0%
!!! TATGACAATTCTGTTTTGGC+AGG - chr5_4:21176345-21176364 Msa0843530:CDS 35.0%
!!! TGTTTTGGCAGGAGTAAAAT+GGG - chr5_4:21176356-21176375 Msa0843530:CDS 35.0%
AGTAAAATGGGTAATCAGAG+TGG - chr5_4:21176368-21176387 Msa0843530:CDS 35.0%
AGATCAAATTCTGTTGCATC+CGG - chr5_4:21176403-21176422 Msa0843530:CDS 35.0%
AGGCTTAAGTGTATTCTCTT+AGG - chr5_4:21176468-21176487 Msa0843530:CDS 35.0%
GGCTTAAGTGTATTCTCTTA+GGG - chr5_4:21176469-21176488 Msa0843530:CDS 35.0%
TTAAGTGTATTCTCTTAGGG+AGG - chr5_4:21176472-21176491 Msa0843530:CDS 35.0%
CACAACGTACATAAGGATTC+TGG + chr5_4:21173467-21173486 None:intergenic 40.0%
CAGAATCCTTATGTACGTTG+TGG - chr5_4:21173465-21173484 Msa0843530:exon 40.0%
! CCAGACTATTAGGCAACTTT+TGG + chr5_4:21173529-21173548 None:intergenic 40.0%
CCAAAAGTTGCCTAATAGTC+TGG - chr5_4:21173526-21173545 Msa0843530:CDS 40.0%
ATGCCAACATTCTTACATGC+GGG + chr5_4:21173613-21173632 None:intergenic 40.0%
GATGCCAACATTCTTACATG+CGG + chr5_4:21173614-21173633 None:intergenic 40.0%
! GCTTGACATATTAGCTTCCT+TGG + chr5_4:21173765-21173784 None:intergenic 40.0%
TAATATGTCAAGCAAGTGCG+TGG - chr5_4:21173772-21173791 Msa0843530:intron 40.0%
CAACTTCGAAGTCTGTATGT+TGG - chr5_4:21173887-21173906 Msa0843530:intron 40.0%
TAGGACTAGACATCCATTCT+GGG + chr5_4:21173934-21173953 None:intergenic 40.0%
GTTTACACCTTCCACATTTG+GGG - chr5_4:21173999-21174018 Msa0843530:intron 40.0%
CTTGATAAGGCTATCAGGTT+TGG + chr5_4:21174154-21174173 None:intergenic 40.0%
!! CAAACCTGATAGCCTTATCA+AGG - chr5_4:21174152-21174171 Msa0843530:intron 40.0%
AAAGGCAAGCCCAACAATTT+AGG + chr5_4:21174232-21174251 None:intergenic 40.0%
TCAATGAGAGATGGAAGGAA+AGG + chr5_4:21174250-21174269 None:intergenic 40.0%
CCATATCAATGAGAGATGGA+AGG + chr5_4:21174255-21174274 None:intergenic 40.0%
CCTTCCATCTCTCATTGATA+TGG - chr5_4:21174252-21174271 Msa0843530:intron 40.0%
! AGCAAGTTAGCTCTTCATTG+TGG + chr5_4:21174354-21174373 None:intergenic 40.0%
!! AGCTAACTTGCTTTCCAGAT+GGG - chr5_4:21174362-21174381 Msa0843530:intron 40.0%
CTTCATCTGTCAATGACTTC+AGG + chr5_4:21174513-21174532 None:intergenic 40.0%
GAATCTTGCTCAGGAATAGA+AGG - chr5_4:21174571-21174590 Msa0843530:intron 40.0%
AGGAATAGAAGGTTTACACC+CGG - chr5_4:21174582-21174601 Msa0843530:intron 40.0%
CACTCAATGCCAATGATTGA+AGG + chr5_4:21174627-21174646 None:intergenic 40.0%
ATTTGTGGTTGCAGTGAGTT+AGG - chr5_4:21174784-21174803 Msa0843530:intron 40.0%
TTTGTGGTTGCAGTGAGTTA+GGG - chr5_4:21174785-21174804 Msa0843530:intron 40.0%
GAACGATGTAAAGAGAACAC+AGG - chr5_4:21174808-21174827 Msa0843530:intron 40.0%
ATGTAAAGAGAACACAGGTG+AGG - chr5_4:21174813-21174832 Msa0843530:intron 40.0%
GAAAACAGAGGAATGTTCTG+CGG - chr5_4:21174874-21174893 Msa0843530:intron 40.0%
! GAGGATTTTATTGTGCAGCT+TGG - chr5_4:21174905-21174924 Msa0843530:intron 40.0%
AAATCTAAACACCAGACCTG+TGG + chr5_4:21175164-21175183 None:intergenic 40.0%
! TGTCATTTCCTTGTTCAACC+CGG - chr5_4:21175240-21175259 Msa0843530:intron 40.0%
!! ATATTTTCCCGGGTTGAACA+AGG + chr5_4:21175251-21175270 None:intergenic 40.0%
AACCCGGGAAAATATACAAC+TGG - chr5_4:21175256-21175275 Msa0843530:intron 40.0%
ACCCGGGAAAATATACAACT+GGG - chr5_4:21175257-21175276 Msa0843530:intron 40.0%
! ACCCAGTTGTATATTTTCCC+GGG + chr5_4:21175261-21175280 None:intergenic 40.0%
TATAATGCTACAATTGGCGC+TGG + chr5_4:21175295-21175314 None:intergenic 40.0%
TACCCACGTAAGCAAAGAAA+AGG + chr5_4:21175321-21175340 None:intergenic 40.0%
AATTTCGCACGAAGTTGTTG+AGG + chr5_4:21175632-21175651 None:intergenic 40.0%
TTGAATGTCCCAAGTAAGAG+AGG - chr5_4:21175800-21175819 Msa0843530:CDS 40.0%
CTCCAATACCTCTCTTACTT+GGG + chr5_4:21175811-21175830 None:intergenic 40.0%
GCTCACAGGATTGCTAAATA+AGG + chr5_4:21175845-21175864 None:intergenic 40.0%
TAGATTTAGCTGTGGGAATG+TGG - chr5_4:21175954-21175973 Msa0843530:intron 40.0%
!!! AGTTTTTTAGGCGTAATCGC+TGG - chr5_4:21176112-21176131 Msa0843530:CDS 40.0%
AACATGTTAACTCAGTCTCG+CGG + chr5_4:21176198-21176217 None:intergenic 40.0%
GTTGAATGGGGTATATTGAC+AGG + chr5_4:21176229-21176248 None:intergenic 40.0%
!!! CTGTTTTGGCAGGAGTAAAA+TGG - chr5_4:21176355-21176374 Msa0843530:CDS 40.0%
TCAATACATGGGGTGAGAGT+TGG + chr5_4:21173328-21173347 None:intergenic 45.0%
TGCCAACATTCTTACATGCG+GGG + chr5_4:21173612-21173631 None:intergenic 45.0%
CTTCGAAGTTGCTGAGTTTG+AGG + chr5_4:21173878-21173897 None:intergenic 45.0%
CTTCGAAGTCTGTATGTTGG+AGG - chr5_4:21173890-21173909 Msa0843530:intron 45.0%
CTGTATGTTGGAGGATACAC+CGG - chr5_4:21173899-21173918 Msa0843530:intron 45.0%
! AGACATCCATTCTGGGAAAC+AGG + chr5_4:21173927-21173946 None:intergenic 45.0%
GTAGGACTAGACATCCATTC+TGG + chr5_4:21173935-21173954 None:intergenic 45.0%
GTAGTTTCCCCAAATGTGGA+AGG + chr5_4:21174009-21174028 None:intergenic 45.0%
GATGGTAGTTTCCCCAAATG+TGG + chr5_4:21174013-21174032 None:intergenic 45.0%
ACCTCCTGCAACTCCATTAT+GGG + chr5_4:21174095-21174114 None:intergenic 45.0%
AACCTCCTGCAACTCCATTA+TGG + chr5_4:21174096-21174115 None:intergenic 45.0%
CATCCCTTGATAAGGCTATC+AGG + chr5_4:21174159-21174178 None:intergenic 45.0%
!! GAGCTAACTTGCTTTCCAGA+TGG - chr5_4:21174361-21174380 Msa0843530:intron 45.0%
AGGTTTCTTAGCATCCCATC+TGG + chr5_4:21174379-21174398 None:intergenic 45.0%
AGATGGGATGCTAAGAAACC+TGG - chr5_4:21174378-21174397 Msa0843530:intron 45.0%
GGATGGGAATTGCTCAAGTT+TGG + chr5_4:21174437-21174456 None:intergenic 45.0%
GTTTCCTAACCAGTCAGGTA+GGG + chr5_4:21174668-21174687 None:intergenic 45.0%
CTTAGGTTTCCTAACCAGTC+AGG + chr5_4:21174673-21174692 None:intergenic 45.0%
!! GTAAGGCATTGAATGCTCGT+CGG + chr5_4:21174745-21174764 None:intergenic 45.0%
ACGAGCATTCAATGCCTTAC+TGG - chr5_4:21174745-21174764 Msa0843530:intron 45.0%
TTGTGGTTGCAGTGAGTTAG+GGG - chr5_4:21174786-21174805 Msa0843530:intron 45.0%
AAGAGAACACAGGTGAGGAT+TGG - chr5_4:21174818-21174837 Msa0843530:intron 45.0%
TATACTCACGTGAGAAGTGG+TGG - chr5_4:21175002-21175021 Msa0843530:intron 45.0%
ATACTCACGTGAGAAGTGGT+GGG - chr5_4:21175003-21175022 Msa0843530:intron 45.0%
GTCATTTCCTTGTTCAACCC+GGG - chr5_4:21175241-21175260 Msa0843530:intron 45.0%
! TCGCCTTTTCTTTGCTTACG+TGG - chr5_4:21175315-21175334 Msa0843530:intron 45.0%
! CGCCTTTTCTTTGCTTACGT+GGG - chr5_4:21175316-21175335 Msa0843530:intron 45.0%
!!! GGCAGATGAACTTTTTGGGT+CGG + chr5_4:21175699-21175718 None:intergenic 45.0%
! GTCCCAAGTAAGAGAGGTAT+TGG - chr5_4:21175806-21175825 Msa0843530:CDS 45.0%
GCTCCAATACCTCTCTTACT+TGG + chr5_4:21175812-21175831 None:intergenic 45.0%
! ACTACTGATTGGATGCTCAC+AGG + chr5_4:21175859-21175878 None:intergenic 45.0%
GAAAGTAGGGCTGCTATAGA+TGG - chr5_4:21176011-21176030 Msa0843530:intron 45.0%
ATGGGTAATCAGAGTGGACA+AGG - chr5_4:21176374-21176393 Msa0843530:CDS 45.0%
GACATCCTCTTGTTCAAGAC+CGG + chr5_4:21176425-21176444 None:intergenic 45.0%
!! ATAATAATTCTAATAAAATA+GGG + chr5_4:21176593-21176612 None:intergenic 5.0%
CTCGAAGTCAAGCACTCGTA+AGG + chr5_4:21173360-21173379 None:intergenic 50.0%
GCCAACATTCTTACATGCGG+GGG + chr5_4:21173611-21173630 None:intergenic 50.0%
! CCATTCTGGGAAACAGGAAC+CGG + chr5_4:21173921-21173940 None:intergenic 50.0%
! TCGTCCCATAATGGAGTTGC+AGG - chr5_4:21174088-21174107 Msa0843530:intron 50.0%
! TCCCATAATGGAGTTGCAGG+AGG - chr5_4:21174091-21174110 Msa0843530:intron 50.0%
TCAGGTAGGGATGCTAGGTT+TGG + chr5_4:21174655-21174674 None:intergenic 50.0%
GGTTTCCTAACCAGTCAGGT+AGG + chr5_4:21174669-21174688 None:intergenic 50.0%
AACAGAGGAATGTTCTGCGG+AGG - chr5_4:21174877-21174896 Msa0843530:intron 50.0%
!!! TTTTTTTCCCTGGGGGGTTG+GGG + chr5_4:21176165-21176184 None:intergenic 50.0%
!!! CTTTTTTTCCCTGGGGGGTT+GGG + chr5_4:21176166-21176185 None:intergenic 50.0%
!!! ACTTTTTTTCCCTGGGGGGT+TGG + chr5_4:21176167-21176186 None:intergenic 50.0%
TGCATCCGGTCTTGAACAAG+AGG - chr5_4:21176417-21176436 Msa0843530:CDS 50.0%
GCCCCCGCATGTAAGAATGT+TGG - chr5_4:21173607-21173626 Msa0843530:intron 55.0%
CCGGTTCCTGTTTCCCAGAA+TGG - chr5_4:21173918-21173937 Msa0843530:intron 55.0%
GACGAGGAGTCGTCCCATAA+TGG - chr5_4:21174079-21174098 Msa0843530:intron 55.0%
ACCTAGCATCCCTACCTGAC+TGG - chr5_4:21174656-21174675 Msa0843530:intron 55.0%
ACCAGTCAGGTAGGGATGCT+AGG + chr5_4:21174660-21174679 None:intergenic 55.0%
! GCATCCCTACCTGACTGGTT+AGG - chr5_4:21174661-21174680 Msa0843530:intron 55.0%
GTCGGAAGACACGTCAGCTT+TGG + chr5_4:21174727-21174746 None:intergenic 55.0%
AGAGGAATGTTCTGCGGAGG+AGG - chr5_4:21174880-21174899 Msa0843530:intron 55.0%
!!! TTTTTTCCCTGGGGGGTTGG+GGG + chr5_4:21176164-21176183 None:intergenic 55.0%
GGAATGTTCTGCGGAGGAGG+AGG - chr5_4:21174883-21174902 Msa0843530:intron 60.0%
ATGTTCTGCGGAGGAGGAGG+AGG - chr5_4:21174886-21174905 Msa0843530:intron 60.0%
! TTTTTCCCTGGGGGGTTGGG+GGG + chr5_4:21176163-21176182 None:intergenic 60.0%
AGTAATCCCCCCAACCCCCC+AGG - chr5_4:21176154-21176173 Msa0843530:CDS 65.0%
GTAATCCCCCCAACCCCCCA+GGG - chr5_4:21176155-21176174 Msa0843530:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 21173254 21176703 21173254 ID=Msa0843530;Name=Msa0843530
chr5_4 mRNA 21173254 21176703 21173254 ID=Msa0843530-mRNA-1;Parent=Msa0843530;Name=Msa0843530-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|0|0.16|1|0|0.16|6|228|393
chr5_4 exon 21173254 21173590 21173254 ID=Msa0843530-mRNA-1:exon:8776;Parent=Msa0843530-mRNA-1
chr5_4 exon 21175442 21175673 21175442 ID=Msa0843530-mRNA-1:exon:8775;Parent=Msa0843530-mRNA-1
chr5_4 exon 21175683 21175907 21175683 ID=Msa0843530-mRNA-1:exon:8774;Parent=Msa0843530-mRNA-1
chr5_4 exon 21176037 21176267 21176037 ID=Msa0843530-mRNA-1:exon:8773;Parent=Msa0843530-mRNA-1
chr5_4 exon 21176283 21176530 21176283 ID=Msa0843530-mRNA-1:exon:8772;Parent=Msa0843530-mRNA-1
chr5_4 exon 21176567 21176703 21176567 ID=Msa0843530-mRNA-1:exon:8771;Parent=Msa0843530-mRNA-1
chr5_4 CDS 21176567 21176703 21176567 ID=Msa0843530-mRNA-1:cds;Parent=Msa0843530-mRNA-1
chr5_4 CDS 21176283 21176530 21176283 ID=Msa0843530-mRNA-1:cds;Parent=Msa0843530-mRNA-1
chr5_4 CDS 21176037 21176267 21176037 ID=Msa0843530-mRNA-1:cds;Parent=Msa0843530-mRNA-1
chr5_4 CDS 21175683 21175907 21175683 ID=Msa0843530-mRNA-1:cds;Parent=Msa0843530-mRNA-1
chr5_4 CDS 21175442 21175673 21175442 ID=Msa0843530-mRNA-1:cds;Parent=Msa0843530-mRNA-1
chr5_4 CDS 21173482 21173590 21173482 ID=Msa0843530-mRNA-1:cds;Parent=Msa0843530-mRNA-1
chr5_4 three_prime_UTR 21173254 21173481 21173254 ID=Msa0843530-mRNA-1:three_prime_utr;Parent=Msa0843530-mRNA-1
Gene Sequence

>Msa0843530

ATGTTAGAGGAAGTTGGTTCAATCACGTTACACTGTGTTGAATCATTAAAAACATGTATAAATTGTGTTGACCAACTCTCACCCCATGTATTGAGATGTTATTCCTTACGAGTGCTTGACTTCGAGAGCAAAGAAAAGTACTTGAATCTTTCTTATGGATATTTCAAAACTCTTCCAGAATCCTTATGTACGTTGTGGAATTTGCAGATTTTAAAGTTAGACTATTGTCGAGATCTCCAAAAGTTGCCTAATAGTCTGGTACACTTGAAATCTCTACAACATCTATATTTGAGACGCTGCAATTCTCTATCAAGCTTGCCCCCGCATGTAAGAATGTTGGCATCCTTGAAAATTTTAACACTATATGTAGTTGGCATGAAAAAAGAGTTAGGACAAATGAACCTTCAAGGAGACCTTTACATTAAGCACTTGGAGAGAGTTAAAAGTGTAATGAATGCCAAGGAAGCTAATATGTCAAGCAAGTGCGTGGACAATTTGAAATTGTCATGGGATAGAAATGAAGTGTCACAAGTACAGGAAAATGTTGAGGAGATTCTTGAAGTGCTTCAACCTCAAACTCAGCAACTTCGAAGTCTGTATGTTGGAGGATACACCGTATCTAGTATGAGTCATGTAAAATACTTAGACGAGGAGTCGTCCCATAATGGAGTTGCAGGAGGTTTCATACGTCTAGAATGTCTGATTCTAGAGAAGCTGCCAAACCTGATAGCCTTATCAAGGGATGATAGAGAAAACATGCTTCCACACCTTTCTAAATTTCAAATTACTGAATGTCCTAAATTGTTGGGCTTGCCTTTCCTTCCATCTCTCATTGATATGGGAAAATGCAACACGGGTTTACTAACTTCAATTCATAAACTTGTTAGTCTTGAATCCCTTATGTTCAACCACAATGAAGAGCTAACTTGCTTTCCAGATGGGATGCTAAGAAACCTGGCTTCTCTAAAAACATTTGACATTTGTGGGATTTCCAAACTTGAGCAATTCCCATCCGAAATCTTTAACATTAACACCATCCAAGAAATATATATCAAAAAATGTGATAACCTGAAGAGTAAAATGGGTAATCAGAGTGGACAAGGACATGAAGATCAAATTCTGTTGCATCCGGTCTTGAACAAGAGGATGTCCTACATTGCTTTTTATTCATTCAAGGCTTAA
Protein sequence

>Msa0843530

MLEEVGSITLHCVESLKTCINCVDQLSPHVLRCYSLRVLDFESKEKYLNLSYGYFKTLPESLCTLWNLQILKLDYCRDLQKLPNSLVHLKSLQHLYLRRCNSLSSLPPHVRMLASLKILTLYVVGMKKELGQMNLQGDLYIKHLERVKSVMNAKEANMSSKCVDNLKLSWDRNEVSQVQENVEEILEVLQPQTQQLRSLYVGGYTVSSMSHVKYLDEESSHNGVAGGFIRLECLILEKLPNLIALSRDDRENMLPHLSKFQITECPKLLGLPFLPSLIDMGKCNTGLLTSIHKLVSLESLMFNHNEELTCFPDGMLRNLASLKTFDICGISKLEQFPSEIFNINTIQEIYIKKCDNLKSKMGNQSGQGHEDQILLHPVLNKRMSYIAFYSFKA*