AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0806070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806070 MtrunA17_Chr5g0414201 67.626 417 70 5 5 358 534 948 7.82e-175 512
Msa0806070 MtrunA17_Chr5g0414181 57.720 421 115 4 2 359 529 949 5.74e-145 436
Msa0806070 MtrunA17_Chr3g0087301 54.286 420 125 5 7 359 519 938 3.60e-130 400
Msa0806070 MtrunA17_Chr5g0414211 67.730 282 44 3 133 368 1 281 1.37e-117 348
Msa0806070 MtrunA17_Chr5g0414161 49.622 397 126 8 4 334 21 409 1.62e-105 317
Msa0806070 MtrunA17_Chr5g0415471 48.471 425 145 10 4 358 535 955 9.15e-100 320
Msa0806070 MtrunA17_Chr5g0414311 45.327 428 161 8 2 359 535 959 5.25e-99 318
Msa0806070 MtrunA17_Chr5g0414301 53.509 342 133 6 46 363 144 483 7.62e-97 300
Msa0806070 MtrunA17_Chr5g0417251 51.240 363 107 6 3 296 297 658 5.64e-95 297
Msa0806070 MtrunA17_Chr5g0415481 43.288 365 170 10 2 333 99 459 3.18e-69 225
Msa0806070 MtrunA17_Chr5g0413811 43.917 337 135 11 30 335 334 647 1.49e-62 212
Msa0806070 MtrunA17_Chr5g0414221 77.692 130 17 1 4 121 18 147 1.66e-59 189
Msa0806070 MtrunA17_Chr5g0430721 34.699 366 184 10 46 359 621 983 8.67e-45 166
Msa0806070 MtrunA17_Chr5g0430291 34.605 367 184 9 46 359 582 945 1.20e-44 165
Msa0806070 MtrunA17_Chr5g0430141 34.204 383 196 11 46 376 584 962 2.96e-44 164
Msa0806070 MtrunA17_Chr5g0430341 33.333 363 189 9 46 358 126 485 3.23e-44 160
Msa0806070 MtrunA17_Chr4g0027251 34.135 416 202 12 13 359 536 948 1.26e-43 162
Msa0806070 MtrunA17_Chr7g0244231 33.699 365 189 9 46 359 574 936 1.82e-43 162
Msa0806070 MtrunA17_Chr5g0429961 33.242 364 188 9 46 358 579 938 4.14e-43 160
Msa0806070 MtrunA17_Chr5g0430441 32.117 411 210 11 13 358 535 941 5.67e-43 160
Msa0806070 MtrunA17_Chr6g0470001 32.970 367 177 9 46 359 186 536 7.18e-43 159
Msa0806070 MtrunA17_Chr1g0155321 34.677 372 178 11 34 343 555 923 1.28e-42 159
Msa0806070 MtrunA17_Chr6g0470011 31.773 406 198 11 7 359 550 929 4.32e-42 158
Msa0806070 MtrunA17_Chr6g0470021 31.931 404 195 12 9 359 442 818 4.33e-42 157
Msa0806070 MtrunA17_Chr5g0430621 34.140 372 193 10 46 368 581 949 9.71e-42 157
Msa0806070 MtrunA17_Chr5g0430581 32.843 408 209 11 13 358 530 934 3.69e-41 155
Msa0806070 MtrunA17_Chr5g0431051 34.341 364 184 10 46 358 584 943 4.94e-41 154
Msa0806070 MtrunA17_Chr6g0470081 31.880 367 181 9 46 359 579 929 4.32e-40 152
Msa0806070 MtrunA17_Chr4g0071011 33.242 364 190 10 46 359 73 433 4.73e-40 149
Msa0806070 MtrunA17_Chr5g0430511 31.675 382 191 9 46 358 583 963 1.08e-38 147
Msa0806070 MtrunA17_Chr1g0155991 34.625 387 187 12 34 358 557 939 6.48e-38 145
Msa0806070 MtrunA17_Chr5g0430641 31.771 384 206 11 27 358 562 941 1.15e-36 142
Msa0806070 MtrunA17_Chr5g0429951 30.831 373 202 9 46 363 583 954 2.10e-36 141
Msa0806070 MtrunA17_Chr8g0351811 30.435 414 210 13 13 359 54 456 3.54e-33 131
Msa0806070 MtrunA17_Chr6g0467911 45.665 173 86 4 46 211 579 750 5.73e-32 127
Msa0806070 MtrunA17_Chr5g0430901 31.680 363 166 11 42 359 497 822 1.31e-30 124
Msa0806070 MtrunA17_Chr3g0085931 29.891 368 194 9 46 359 577 934 2.92e-30 123
Msa0806070 MtrunA17_Chr6g0468151 28.099 363 194 6 46 359 581 925 3.61e-30 122
Msa0806070 MtrunA17_Chr6g0468121 30.000 370 197 10 46 359 576 939 6.29e-30 122
Msa0806070 MtrunA17_Chr3g0123111 29.428 367 194 9 46 359 574 928 4.86e-29 119
Msa0806070 MtrunA17_Chr6g0468101 27.250 400 199 8 46 354 536 934 5.42e-29 119
Msa0806070 MtrunA17_Chr5g0430951 32.424 330 159 11 13 280 535 862 2.70e-27 114
Msa0806070 MtrunA17_Chr6g0468201 29.132 357 195 9 57 359 191 543 4.12e-27 113
Msa0806070 MtrunA17_Chr6g0467961 31.627 332 161 8 8 277 523 850 2.54e-26 111
Msa0806070 MtrunA17_Chr6g0468271 28.142 366 202 8 46 359 572 928 3.73e-26 110
Msa0806070 MtrunA17_Chr6g0468451 28.261 368 201 9 46 359 575 933 1.98e-25 108
Msa0806070 MtrunA17_Chr6g0468021 29.348 368 202 9 46 359 573 936 6.89e-24 104
Msa0806070 MtrunA17_Chr5g0410301 26.735 389 202 12 46 357 572 954 1.86e-22 99.8
Msa0806070 MtrunA17_Chr6g0468361 31.174 247 120 5 46 242 184 430 4.29e-22 97.8
Msa0806070 MtrunA17_Chr3g0089651 30.488 328 184 15 46 357 607 906 2.57e-21 96.7
Msa0806070 MtrunA17_Chr2g0282991 39.247 186 100 7 46 222 599 780 3.00e-21 96.3
Msa0806070 MtrunA17_Chr3g0089251 30.581 327 185 15 46 357 632 931 4.93e-21 95.5
Msa0806070 MtrunA17_Chr3g0089211 29.969 327 198 13 46 351 650 966 6.33e-21 95.1
Msa0806070 MtrunA17_Chr3g0089761 31.212 330 178 16 46 357 603 901 7.66e-21 95.1
Msa0806070 MtrunA17_Chr3g0089781 30.088 339 205 14 46 362 603 931 2.29e-20 93.6
Msa0806070 MtrunA17_Chr3g0089811 31.707 328 180 16 46 357 603 902 4.42e-20 92.8
Msa0806070 MtrunA17_Chr5g0410161 30.000 280 123 10 46 268 717 980 8.46e-20 92.0
Msa0806070 MtrunA17_Chr3g0087701 29.912 341 179 14 46 357 593 902 1.27e-19 91.3
Msa0806070 MtrunA17_Chr3g0089421 29.664 327 188 15 46 357 605 904 1.80e-19 90.9
Msa0806070 MtrunA17_Chr3g0092241 31.003 329 187 16 46 360 603 905 6.21e-19 89.4
Msa0806070 MtrunA17_Chr3g0089601 30.183 328 185 15 46 357 601 900 7.06e-19 89.0
Msa0806070 MtrunA17_Chr2g0309721 29.834 362 200 16 23 357 565 899 1.30e-18 88.2
Msa0806070 MtrunA17_Chr4g0025391 30.321 343 179 16 46 352 601 919 1.46e-18 88.2
Msa0806070 MtrunA17_Chr3g0089471 30.793 328 183 15 46 357 651 950 1.59e-18 87.8
Msa0806070 MtrunA17_Chr3g0086221 30.588 340 170 18 46 357 588 889 2.32e-18 87.4
Msa0806070 MtrunA17_Chr3g0089441 28.286 350 197 13 46 362 109 437 3.22e-18 86.7
Msa0806070 MtrunA17_Chr3g0090691 25.000 400 206 14 46 357 604 997 3.32e-18 87.0
Msa0806070 MtrunA17_Chr3g0085751 29.738 343 181 14 46 357 589 902 3.48e-18 87.0
Msa0806070 MtrunA17_Chr3g0089501 35.106 188 108 7 46 225 601 782 3.91e-18 86.7
Msa0806070 MtrunA17_Chr3g0089541 29.573 328 187 15 46 357 606 905 3.96e-18 86.7
Msa0806070 MtrunA17_Chr5g0446611 35.870 184 106 6 47 222 604 783 3.97e-18 86.7
Msa0806070 MtrunA17_Chr3g0086621 28.824 340 184 14 46 357 589 898 5.51e-18 86.3
Msa0806070 MtrunA17_Chr5g0410401 27.320 388 182 18 46 359 446 807 7.46e-18 85.9
Msa0806070 MtrunA17_Chr5g0410481 26.044 407 214 13 46 368 560 963 1.10e-17 85.5
Msa0806070 MtrunA17_Chr5g0410351 25.610 410 215 14 46 368 570 976 1.12e-17 85.5
Msa0806070 MtrunA17_Chr3g0082131 28.117 377 191 16 46 358 611 971 1.30e-17 85.1
Msa0806070 MtrunA17_Chr3g0086161 28.448 348 177 16 46 357 589 900 1.34e-17 85.1
Msa0806070 MtrunA17_Chr3g0086151 31.602 231 132 10 46 268 589 801 1.48e-17 84.7
Msa0806070 MtrunA17_Chr1g0164581 28.117 377 191 16 46 358 587 947 1.55e-17 85.1
Msa0806070 MtrunA17_Chr3g0085711 29.825 342 182 15 46 357 161 474 2.34e-17 84.0
Msa0806070 MtrunA17_Chr3g0092261 30.606 330 187 16 46 360 647 949 2.70e-17 84.3
Msa0806070 MtrunA17_Chr3g0086091 34.682 173 104 5 46 211 400 570 3.00e-17 84.0
Msa0806070 MtrunA17_Chr3g0090651 27.103 321 168 11 46 303 604 921 3.37e-17 84.0
Msa0806070 MtrunA17_Chr7g0255751 38.926 149 82 4 46 185 62 210 5.26e-17 79.7
Msa0806070 MtrunA17_Chr3g0086071 28.395 324 171 13 46 361 209 479 5.73e-17 82.8
Msa0806070 MtrunA17_Chr3g0089991 30.605 281 146 12 27 264 560 834 7.76e-17 82.8
Msa0806070 MtrunA17_Chr7g0248531 29.674 337 180 14 39 364 583 873 1.00e-16 82.4
Msa0806070 MtrunA17_Chr3g0091061 25.000 400 207 14 46 357 604 998 1.08e-16 82.4
Msa0806070 MtrunA17_Chr3g0085651 28.367 349 182 16 46 361 599 912 1.12e-16 82.4
Msa0806070 MtrunA17_Chr3g0084971 30.128 312 158 11 46 300 594 902 1.17e-16 82.4
Msa0806070 MtrunA17_Chr5g0410261 30.935 278 150 11 34 270 577 853 1.22e-16 82.0
Msa0806070 MtrunA17_Chr3g0086181 34.503 171 99 6 46 209 589 753 1.24e-16 82.0
Msa0806070 MtrunA17_Chr3g0085551 35.838 173 100 6 46 209 481 651 2.59e-16 80.9
Msa0806070 MtrunA17_Chr3g0091011 28.863 343 184 14 46 357 573 886 2.79e-16 80.9
Msa0806070 MtrunA17_Chr3g0085091 34.682 173 102 6 46 209 597 767 3.68e-16 80.9
Msa0806070 MtrunA17_Chr8g0356251 28.117 377 196 17 46 358 627 992 3.95e-16 80.5
Msa0806070 MtrunA17_Chr3g0089871 34.286 210 121 9 46 245 606 808 4.07e-16 80.5
Msa0806070 MtrunA17_Chr3g0082391 36.095 169 93 7 46 204 606 769 4.16e-16 80.5
Msa0806070 MtrunA17_Chr3g0087841 27.301 326 196 14 46 357 603 901 5.57e-16 80.1
Msa0806070 MtrunA17_Chr3g0091081 27.103 321 167 12 46 303 602 918 6.82e-16 79.7
Msa0806070 MtrunA17_Chr3g0090431 31.383 188 115 7 46 225 475 656 7.30e-16 79.7
Msa0806070 MtrunA17_Chr3g0085131 28.440 327 163 16 46 361 591 857 1.66e-15 78.6
Msa0806070 MtrunA17_Chr3g0082311 36.527 167 93 7 46 204 605 766 2.03e-15 78.6
Msa0806070 MtrunA17_Chr3g0090371 31.053 190 113 7 46 225 602 783 2.86e-15 77.8
Msa0806070 MtrunA17_Chr3g0085171 34.682 173 102 6 46 209 581 751 3.16e-15 77.8
Msa0806070 MtrunA17_Chr3g0083751 38.012 171 90 9 46 204 606 772 3.35e-15 77.8
Msa0806070 MtrunA17_Chr3g0083621 35.119 168 99 6 46 205 612 777 4.24e-15 77.4
Msa0806070 MtrunA17_Chr7g0221481 35.838 173 98 7 46 209 600 768 4.43e-15 77.4
Msa0806070 MtrunA17_Chr3g0085521 34.161 161 96 5 46 197 586 745 4.65e-15 77.4
Msa0806070 MtrunA17_Chr8g0352831 33.533 167 99 5 46 204 620 782 5.50e-15 77.0
Msa0806070 MtrunA17_Chr3g0085801 31.950 241 133 11 46 273 571 793 5.53e-15 77.0
Msa0806070 MtrunA17_Chr3g0089961 26.300 327 185 10 46 361 606 887 5.62e-15 77.0
Msa0806070 MtrunA17_Chr3g0083641 37.427 171 91 8 46 204 577 743 6.76e-15 76.6
Msa0806070 MtrunA17_Chr3g0086741 34.682 173 102 6 46 209 618 788 7.69e-15 76.6
Msa0806070 MtrunA17_Chr3g0084051 32.479 234 126 12 46 268 605 817 8.96e-15 76.3
Msa0806070 MtrunA17_Chr1g0164391 34.857 175 96 7 46 204 606 778 9.81e-15 76.3
Msa0806070 MtrunA17_Chr5g0415551 36.585 164 97 6 46 204 461 622 1.04e-14 75.9
Msa0806070 MtrunA17_Chr6g0484551 34.884 172 100 6 46 209 577 744 1.11e-14 75.9
Msa0806070 MtrunA17_Chr3g0082351 37.356 174 94 8 46 209 603 771 1.41e-14 75.9
Msa0806070 MtrunA17_Chr3g0083721 35.928 167 97 6 46 204 544 708 1.97e-14 75.1
Msa0806070 MtrunA17_Chr3g0091121 26.500 400 200 18 46 357 214 607 3.71e-14 74.3
Msa0806070 MtrunA17_Chr3g0085111 26.524 328 174 12 46 361 592 864 3.74e-14 74.3
Msa0806070 MtrunA17_Chr8g0392511 33.140 172 103 6 46 209 164 331 3.86e-14 73.2
Msa0806070 MtrunA17_Chr3g0087541 27.859 341 154 17 46 358 604 880 4.42e-14 74.3
Msa0806070 MtrunA17_Chr3g0085561 27.078 373 210 14 46 361 584 951 4.52e-14 74.3
Msa0806070 MtrunA17_Chr3g0082811 33.714 175 101 6 46 209 586 756 5.53e-14 73.9
Msa0806070 MtrunA17_Chr3g0089591 28.075 374 180 16 46 357 607 953 5.72e-14 73.9
Msa0806070 MtrunA17_Chr3g0090141 31.818 154 94 5 46 191 603 753 7.62e-14 73.6
Msa0806070 MtrunA17_Chr3g0085161 27.907 344 186 15 46 357 455 768 1.01e-13 73.2
Msa0806070 MtrunA17_Chr3g0082321 35.329 167 95 7 46 204 551 712 1.04e-13 72.8
Msa0806070 MtrunA17_Chr1g0147141 26.617 402 196 18 46 358 605 996 1.16e-13 72.8
Msa0806070 MtrunA17_Chr3g0087581 33.333 171 105 5 46 209 590 758 1.51e-13 72.8
Msa0806070 MtrunA17_Chr8g0348931 31.651 218 134 8 46 254 609 820 1.63e-13 72.4
Msa0806070 MtrunA17_Chr1g0164611 34.043 188 102 9 46 215 615 798 1.97e-13 72.4
Msa0806070 MtrunA17_Chr3g0082101 34.043 188 102 9 46 215 563 746 2.19e-13 72.0
Msa0806070 MtrunA17_Chr3g0083681 35.329 167 98 6 46 204 578 742 4.40e-13 71.2
Msa0806070 MtrunA17_Chr3g0083591 34.568 162 96 6 51 204 571 730 6.77e-13 70.5
Msa0806070 MtrunA17_Chr4g0010201 25.735 408 204 15 46 359 597 999 7.70e-13 70.5
Msa0806070 MtrunA17_Chr2g0299151 25.697 323 188 11 46 361 594 871 8.09e-13 70.5
Msa0806070 MtrunA17_Chr6g0467921 32.258 155 90 4 209 359 1 144 8.62e-13 68.9
Msa0806070 MtrunA17_Chr4g0009781 24.069 403 215 13 46 359 598 998 9.03e-13 70.1
Msa0806070 MtrunA17_Chr3g0085331 30.058 173 109 6 46 211 615 782 9.54e-13 70.1
Msa0806070 MtrunA17_Chr6g0468341 27.402 281 138 9 129 359 472 736 1.35e-12 69.7
Msa0806070 MtrunA17_Chr3g0084031 33.714 175 102 7 46 209 607 778 2.39e-12 68.9
Msa0806070 MtrunA17_Chr4g0020501 25.000 368 191 13 58 358 488 837 5.35e-12 67.8
Msa0806070 MtrunA17_Chr3g0082791 28.267 375 211 18 46 390 606 952 9.86e-12 67.0
Msa0806070 MtrunA17_Chr4g0010221 33.149 181 107 7 46 212 596 776 1.29e-11 66.6
Msa0806070 MtrunA17_Chr2g0316291 26.024 415 198 17 46 357 575 983 1.51e-11 66.2
Msa0806070 MtrunA17_Chr4g0010261 34.932 146 84 5 46 180 598 743 3.20e-11 65.5
Msa0806070 MtrunA17_Chr4g0010251 27.811 338 212 14 46 362 593 919 4.68e-11 64.7
Msa0806070 MtrunA17_Chr3g0085471 26.609 233 129 9 46 265 605 808 5.13e-11 64.7
Msa0806070 MtrunA17_Chr3g0080871 32.967 182 110 7 51 224 594 771 8.46e-11 63.9
Msa0806070 MtrunA17_Chr3g0085501 29.762 168 105 6 46 207 615 775 8.58e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0806070 AT3G14470.1 26.546 388 212 12 43 358 602 988 6.42e-18 86.7
Msa0806070 AT3G14470.2 26.546 388 212 12 43 358 602 988 6.42e-18 86.7
Msa0806070 AT3G14460.1 39.394 132 72 4 46 170 599 729 2.62e-13 72.4

Find 82 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATAATTCTAATAAAAT+AGG 0.122851 5_3:-23521284 None:intergenic
CCTTCCATCTCTCATTGATA+TGG 0.210039 5_3:+23518946 Msa0806070:CDS
AGGCTTAAGTGTATTCTCTT+AGG 0.224893 5_3:+23521161 Msa0806070:exon
AGGTACTTGAATCTTTCTTA+TGG 0.246045 5_3:+23518119 Msa0806070:intron
AACCTCCTGCAACTCCATTA+TGG 0.246508 5_3:-23518787 None:intergenic
AAAGGCAAGCCCAACAATTT+AGG 0.253032 5_3:-23518923 None:intergenic
CCAGACTATTAGGCAACTTT+TGG 0.258593 5_3:-23518220 None:intergenic
GGTGTTAATGTTAAAGATTT+CGG 0.295929 5_3:-23519149 None:intergenic
GGATGGGAATTGCTCAAGTT+TGG 0.326605 5_3:-23519128 None:intergenic
CATCCCTTGATAAGGCTATC+AGG 0.335767 5_3:-23518850 None:intergenic
CTTCGAAGTTGCTGAGTTTG+AGG 0.337667 5_3:-23518569 None:intergenic
TTCAAGTGTACCAGACTATT+AGG 0.343140 5_3:-23518230 None:intergenic
CACAACGTACATAAGGATTC+TGG 0.350495 5_3:-23518158 None:intergenic
ACCTCCTGCAACTCCATTAT+GGG 0.351088 5_3:-23518786 None:intergenic
CTTCATTGTGGTTGAACATA+AGG 0.357595 5_3:-23519033 None:intergenic
TACTGAATGTCCTAAATTGT+TGG 0.361413 5_3:+23518913 Msa0806070:CDS
TTCTCTATCATCCCTTGATA+AGG 0.370077 5_3:-23518858 None:intergenic
CTTCCATCTCTCATTGATAT+GGG 0.376582 5_3:+23518947 Msa0806070:CDS
TCGTCCCATAATGGAGTTGC+AGG 0.387609 5_3:+23518782 Msa0806070:CDS
ATAATAATTCTAATAAAATA+GGG 0.394282 5_3:-23521283 None:intergenic
AAATACTTTGCATTAAGAAT+AGG 0.399908 5_3:-23521362 None:intergenic
ACTGAATGTCCTAAATTGTT+GGG 0.400442 5_3:+23518914 Msa0806070:CDS
GGCTTAAGTGTATTCTCTTA+GGG 0.414020 5_3:+23521162 Msa0806070:exon
CCAAAAGTTGCCTAATAGTC+TGG 0.414031 5_3:+23518220 Msa0806070:CDS
TTGCAGGTGGAACATGTTAG+AGG 0.415349 5_3:+23517935 None:intergenic
CTTGATAAGGCTATCAGGTT+TGG 0.421804 5_3:-23518845 None:intergenic
GACGAGGAGTCGTCCCATAA+TGG 0.428211 5_3:+23518773 Msa0806070:CDS
TTCATTGTGGTTGAACATAA+GGG 0.431182 5_3:-23519032 None:intergenic
TAATGTAAAGGTCTCCTTGA+AGG 0.444130 5_3:-23518400 None:intergenic
GCTTGACATATTAGCTTCCT+TGG 0.444359 5_3:-23518456 None:intergenic
CAGTAATTTGAAATTTAGAA+AGG 0.444658 5_3:-23518895 None:intergenic
CAAATTCCACAACGTACATA+AGG 0.470935 5_3:-23518165 None:intergenic
AGGTTTCTTAGCATCCCATC+TGG 0.475533 5_3:-23519070 None:intergenic
TTTGATATATATTTCTTGGA+TGG 0.485821 5_3:-23519170 None:intergenic
TCTCCAAGTGCTTAATGTAA+AGG 0.491947 5_3:-23518412 None:intergenic
AGGAATAACATCTCAATACA+TGG 0.492791 5_3:-23518031 None:intergenic
CAAACCTGATAGCCTTATCA+AGG 0.493624 5_3:+23518846 Msa0806070:CDS
AAATGAAGTGTCACAAGTAC+AGG 0.494355 5_3:+23518514 Msa0806070:CDS
ATGCCAACATTCTTACATGC+GGG 0.494866 5_3:-23518304 None:intergenic
AGCTAACTTGCTTTCCAGAT+GGG 0.497385 5_3:+23519056 Msa0806070:CDS
AGACCTTTACATTAAGCACT+TGG 0.498417 5_3:+23518409 Msa0806070:CDS
TAAACAGTATGAAAATATCT+TGG 0.501346 5_3:+23521196 Msa0806070:three_prime_UTR
TGGAACATGTTAGAGGAAGT+TGG 0.514806 5_3:+23517942 None:intergenic
AGATCAAATTCTGTTGCATC+CGG 0.525216 5_3:+23521096 Msa0806070:CDS
TTAGGACAAATGAACCTTCA+AGG 0.526890 5_3:+23518386 Msa0806070:CDS
GACATCCTCTTGTTCAAGAC+CGG 0.527277 5_3:-23521115 None:intergenic
TTAATGTTAAAGATTTCGGA+TGG 0.530887 5_3:-23519145 None:intergenic
CTCGAAGTCAAGCACTCGTA+AGG 0.536802 5_3:-23518051 None:intergenic
CTTCGAAGTCTGTATGTTGG+AGG 0.538768 5_3:+23518584 Msa0806070:CDS
GGACAATTTGAAATTGTCAT+GGG 0.548122 5_3:+23518487 Msa0806070:CDS
GAGCTAACTTGCTTTCCAGA+TGG 0.548886 5_3:+23519055 Msa0806070:CDS
CAGAATCCTTATGTACGTTG+TGG 0.551748 5_3:+23518159 Msa0806070:CDS
TCAATACATGGGGTGAGAGT+TGG 0.554333 5_3:-23518019 None:intergenic
AGATGGGATGCTAAGAAACC+TGG 0.563751 5_3:+23519072 Msa0806070:CDS
ATTTGAAATTTAGAAAGGTG+TGG 0.566416 5_3:-23518890 None:intergenic
TGGACAATTTGAAATTGTCA+TGG 0.569957 5_3:+23518486 Msa0806070:CDS
GGAATAACATCTCAATACAT+GGG 0.574024 5_3:-23518030 None:intergenic
TCAATGAGAGATGGAAGGAA+AGG 0.574345 5_3:-23518941 None:intergenic
TCAATAATAACTAAGAAATG+TGG 0.577722 5_3:-23521249 None:intergenic
CAACTTCGAAGTCTGTATGT+TGG 0.580363 5_3:+23518581 Msa0806070:CDS
CATTAAGAATAGGTTTGACA+CGG 0.582049 5_3:-23521352 None:intergenic
TAATGTTAAAGATTTCGGAT+GGG 0.586146 5_3:-23519144 None:intergenic
ATGGGTAATCAGAGTGGACA+AGG 0.589821 5_3:+23521067 Msa0806070:CDS
TGCATCCGGTCTTGAACAAG+AGG 0.595969 5_3:+23521110 Msa0806070:CDS
ATACCCATATCAATGAGAGA+TGG 0.600725 5_3:-23518950 None:intergenic
AGCAAGTTAGCTCTTCATTG+TGG 0.608038 5_3:-23519045 None:intergenic
AGTAAAATGGGTAATCAGAG+TGG 0.614238 5_3:+23521061 Msa0806070:CDS
TAAAAGTGTAATGAATGCCA+AGG 0.617269 5_3:+23518439 Msa0806070:CDS
TGCCAACATTCTTACATGCG+GGG 0.617367 5_3:-23518303 None:intergenic
TCCCATAATGGAGTTGCAGG+AGG 0.618291 5_3:+23518785 Msa0806070:CDS
ACAAGTACAGGAAAATGTTG+AGG 0.624923 5_3:+23518526 Msa0806070:CDS
CCATATCAATGAGAGATGGA+AGG 0.625295 5_3:-23518946 None:intergenic
TTAAGTGTATTCTCTTAGGG+AGG 0.626896 5_3:+23521165 Msa0806070:exon
GCCAACATTCTTACATGCGG+GGG 0.632733 5_3:-23518302 None:intergenic
CTGTATGTTGGAGGATACAC+CGG 0.637156 5_3:+23518593 Msa0806070:CDS
AAACCTGATAGCCTTATCAA+GGG 0.638408 5_3:+23518847 Msa0806070:CDS
ATAAAAGGAAAATGCAACAC+GGG 0.645734 5_3:+23518971 Msa0806070:intron
TAATATGTCAAGCAAGTGCG+TGG 0.652604 5_3:+23518466 Msa0806070:CDS
GAATAACATCTCAATACATG+GGG 0.663735 5_3:-23518029 None:intergenic
GATGCCAACATTCTTACATG+CGG 0.676201 5_3:-23518305 None:intergenic
TATAAAAGGAAAATGCAACA+CGG 0.720090 5_3:+23518970 Msa0806070:intron
TCATGTAAAATACTTAGACG+AGG 0.738566 5_3:+23518757 Msa0806070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTGATATATATTTCT+TGG - chr5_3:23519177-23519196 None:intergenic 10.0%
!!! TTTTCAAATTAAAGTTTTTT+AGG + chr5_3:23520794-23520813 Msa0806070:intron 10.0%
!! CATAATAATTCTAATAAAAT+AGG - chr5_3:23521287-23521306 None:intergenic 10.0%
!!! ATATAGTGTTAAAATTTTCA+AGG - chr5_3:23518330-23518349 None:intergenic 15.0%
!!! TTTTTCATTTTGTTGAATTT+TGG + chr5_3:23519810-23519829 Msa0806070:intron 15.0%
!!! TTTGAAATTTGAAATTTTAG+TGG + chr5_3:23520135-23520154 Msa0806070:intron 15.0%
!!! TGTGATTTATTTTTAATTGT+TGG + chr5_3:23520452-23520471 Msa0806070:intron 15.0%
!! AATGTTGAATGAAATATATT+TGG + chr5_3:23520681-23520700 Msa0806070:intron 15.0%
!! TAAATGTAATACAAAACATT+AGG - chr5_3:23521020-23521039 None:intergenic 15.0%
!!! ATTTTAACACTATATGTAGT+TGG + chr5_3:23518335-23518354 Msa0806070:CDS 20.0%
!!! CTAGTTAAATATTTTAGAGT+AGG - chr5_3:23518647-23518666 None:intergenic 20.0%
!!! CAGTAATTTGAAATTTAGAA+AGG - chr5_3:23518898-23518917 None:intergenic 20.0%
!! TAAAAACATTTGACATTTGT+GGG + chr5_3:23519101-23519120 Msa0806070:CDS 20.0%
!! TTTGATATATATTTCTTGGA+TGG - chr5_3:23519173-23519192 None:intergenic 20.0%
!! AAATTTGTAATGCTTCAAAA+GGG - chr5_3:23519758-23519777 None:intergenic 20.0%
!! AAAATTTGTAATGCTTCAAA+AGG - chr5_3:23519759-23519778 None:intergenic 20.0%
!!! TTTTGAAGCATTACAAATTT+TGG + chr5_3:23519758-23519777 Msa0806070:intron 20.0%
!!! TTTGAAGCATTACAAATTTT+GGG + chr5_3:23519759-23519778 Msa0806070:intron 20.0%
!!! AAACTGAATGATACAAATTT+TGG + chr5_3:23519887-23519906 Msa0806070:intron 20.0%
!!! AACTGAATGATACAAATTTT+GGG + chr5_3:23519888-23519907 Msa0806070:intron 20.0%
!!! TGTACTTTTTGTTTGTTTTA+TGG - chr5_3:23520297-23520316 None:intergenic 20.0%
!! ATACACTGAAAATAACTTAT+TGG - chr5_3:23520578-23520597 None:intergenic 20.0%
!! AAATATATTTGGAGAAAGTA+GGG + chr5_3:23520692-23520711 Msa0806070:intron 20.0%
!! GTGAAATGATATATAACAAT+TGG - chr5_3:23520972-23520991 None:intergenic 20.0%
!! TAAACAGTATGAAAATATCT+TGG + chr5_3:23521196-23521215 Msa0806070:three_prime_UTR 20.0%
!! TCAATAATAACTAAGAAATG+TGG - chr5_3:23521252-23521271 None:intergenic 20.0%
!! AAATACTTTGCATTAAGAAT+AGG - chr5_3:23521365-23521384 None:intergenic 20.0%
!! TTGGTCGTTTAAAATATCTA+AGG + chr5_3:23518099-23518118 Msa0806070:intron 25.0%
!! ATTTGAAATTTAGAAAGGTG+TGG - chr5_3:23518893-23518912 None:intergenic 25.0%
! TATAAAAGGAAAATGCAACA+CGG + chr5_3:23518970-23518989 Msa0806070:intron 25.0%
! CTAAAAACATTTGACATTTG+TGG + chr5_3:23519100-23519119 Msa0806070:CDS 25.0%
! TAATGTTAAAGATTTCGGAT+GGG - chr5_3:23519147-23519166 None:intergenic 25.0%
! TTAATGTTAAAGATTTCGGA+TGG - chr5_3:23519148-23519167 None:intergenic 25.0%
! GGTGTTAATGTTAAAGATTT+CGG - chr5_3:23519152-23519171 None:intergenic 25.0%
! GTAATTCTTGAAGTAAACTT+AGG - chr5_3:23519384-23519403 None:intergenic 25.0%
! CTTAAAAGTTTGAGAATTTG+TGG + chr5_3:23519463-23519482 Msa0806070:intron 25.0%
!!! GGTATTTTAATAGAAAACAG+AGG + chr5_3:23519556-23519575 Msa0806070:intron 25.0%
!!! ACTAGTATAATTTTGAAGTG+AGG + chr5_3:23519724-23519743 Msa0806070:intron 25.0%
!!! CTAGTATAATTTTGAAGTGA+GGG + chr5_3:23519725-23519744 Msa0806070:intron 25.0%
! TATCATCTACTCAATATTCT+TGG + chr5_3:23520037-23520056 Msa0806070:intron 25.0%
! AAATAACAAGCTTGAAATTC+TGG - chr5_3:23520217-23520236 None:intergenic 25.0%
! GAAATATATTTGGAGAAAGT+AGG + chr5_3:23520691-23520710 Msa0806070:intron 25.0%
!! CAAGTTACTTTCTTGTAAAA+TGG - chr5_3:23520760-23520779 None:intergenic 25.0%
!!! ACATTTTACTTTTTTTCCCT+GGG - chr5_3:23520867-23520886 None:intergenic 25.0%
!! CATTTATGACAATTCTGTTT+TGG + chr5_3:23521034-23521053 Msa0806070:intron 25.0%
! GAATGAATAAAAAGCAATGT+AGG - chr5_3:23521140-23521159 None:intergenic 25.0%
!!! CATTGCTTTTTATTCATTCA+AGG + chr5_3:23521141-23521160 Msa0806070:CDS 25.0%
!! TTTTCATACTGTTTAACACT+AGG - chr5_3:23521191-23521210 None:intergenic 25.0%
GAATAACATCTCAATACATG+GGG - chr5_3:23518032-23518051 None:intergenic 30.0%
GGAATAACATCTCAATACAT+GGG - chr5_3:23518033-23518052 None:intergenic 30.0%
AGGAATAACATCTCAATACA+TGG - chr5_3:23518034-23518053 None:intergenic 30.0%
AGGTACTTGAATCTTTCTTA+TGG + chr5_3:23518119-23518138 Msa0806070:intron 30.0%
TATGTAGTTGGCATGAAAAA+AGG + chr5_3:23518347-23518366 Msa0806070:CDS 30.0%
! TAAAAGTGTAATGAATGCCA+AGG + chr5_3:23518439-23518458 Msa0806070:CDS 30.0%
! TGGACAATTTGAAATTGTCA+TGG + chr5_3:23518486-23518505 Msa0806070:CDS 30.0%
! GGACAATTTGAAATTGTCAT+GGG + chr5_3:23518487-23518506 Msa0806070:CDS 30.0%
TCATGTAAAATACTTAGACG+AGG + chr5_3:23518757-23518776 Msa0806070:CDS 30.0%
TACTGAATGTCCTAAATTGT+TGG + chr5_3:23518913-23518932 Msa0806070:CDS 30.0%
ACTGAATGTCCTAAATTGTT+GGG + chr5_3:23518914-23518933 Msa0806070:CDS 30.0%
CTCATTGATATGGGTATAAA+AGG + chr5_3:23518956-23518975 Msa0806070:intron 30.0%
ATAAAAGGAAAATGCAACAC+GGG + chr5_3:23518971-23518990 Msa0806070:intron 30.0%
TTCATTGTGGTTGAACATAA+GGG - chr5_3:23519035-23519054 None:intergenic 30.0%
! TTGACAGATGAAGTTTTACA+AGG + chr5_3:23519214-23519233 Msa0806070:intron 30.0%
! TGACAGATGAAGTTTTACAA+GGG + chr5_3:23519215-23519234 Msa0806070:intron 30.0%
!!! TCAAACTTTTAAGACCAGTA+AGG - chr5_3:23519456-23519475 None:intergenic 30.0%
!!! AGCATTACAAATTTTGGGTT+TGG + chr5_3:23519764-23519783 Msa0806070:intron 30.0%
!!! ATTACAAATTTTGGGTTTGG+TGG + chr5_3:23519767-23519786 Msa0806070:intron 30.0%
!!! TTACAAATTTTGGGTTTGGT+GGG + chr5_3:23519768-23519787 Msa0806070:intron 30.0%
!!! TTGGGTTTTATTTCACTACT+TGG + chr5_3:23519906-23519925 Msa0806070:intron 30.0%
AATAGCTAGAAGAAAATGAC+AGG - chr5_3:23520273-23520292 None:intergenic 30.0%
TCTCAGATGATTAATCATAG+TGG + chr5_3:23520346-23520365 Msa0806070:intron 30.0%
CTCAGATGATTAATCATAGT+GGG + chr5_3:23520347-23520366 Msa0806070:intron 30.0%
TTTAATTGTTGGCGTCAAAA+TGG + chr5_3:23520463-23520482 Msa0806070:intron 30.0%
ACTTATTGGATACTACTGAT+TGG - chr5_3:23520564-23520583 None:intergenic 30.0%
TTGAGAATAGATTTAGCTGT+GGG + chr5_3:23520641-23520660 Msa0806070:intron 30.0%
!!! ATTTTACTTTTTTTCCCTGG+GGG - chr5_3:23520865-23520884 None:intergenic 30.0%
!!! CATTTTACTTTTTTTCCCTG+GGG - chr5_3:23520866-23520885 None:intergenic 30.0%
!!! CACATTTTACTTTTTTTCCC+TGG - chr5_3:23520868-23520887 None:intergenic 30.0%
AATGCACAAGATAGTTGAAT+GGG - chr5_3:23520935-23520954 None:intergenic 30.0%
CATTAAGAATAGGTTTGACA+CGG - chr5_3:23521355-23521374 None:intergenic 30.0%
GAAAAGTTGTCGTCTTCAAT+TGG + chr5_3:23518080-23518099 Msa0806070:intron 35.0%
CAAATTCCACAACGTACATA+AGG - chr5_3:23518168-23518187 None:intergenic 35.0%
TTCAAGTGTACCAGACTATT+AGG - chr5_3:23518233-23518252 None:intergenic 35.0%
! GGTTTTCTTTTAGCAGAGTT+AGG + chr5_3:23518368-23518387 Msa0806070:intron 35.0%
TTAGGACAAATGAACCTTCA+AGG + chr5_3:23518386-23518405 Msa0806070:CDS 35.0%
TAATGTAAAGGTCTCCTTGA+AGG - chr5_3:23518403-23518422 None:intergenic 35.0%
AGACCTTTACATTAAGCACT+TGG + chr5_3:23518409-23518428 Msa0806070:CDS 35.0%
TCTCCAAGTGCTTAATGTAA+AGG - chr5_3:23518415-23518434 None:intergenic 35.0%
! AAATGAAGTGTCACAAGTAC+AGG + chr5_3:23518514-23518533 Msa0806070:CDS 35.0%
ACAAGTACAGGAAAATGTTG+AGG + chr5_3:23518526-23518545 Msa0806070:CDS 35.0%
TTGTTTACACCTTCCACATT+TGG + chr5_3:23518691-23518710 Msa0806070:intron 35.0%
TGTTTACACCTTCCACATTT+GGG + chr5_3:23518692-23518711 Msa0806070:intron 35.0%
! ACTGCTAAACTCTTTAGAGA+TGG - chr5_3:23518725-23518744 None:intergenic 35.0%
!! AAACCTGATAGCCTTATCAA+GGG + chr5_3:23518847-23518866 Msa0806070:CDS 35.0%
TTCTCTATCATCCCTTGATA+AGG - chr5_3:23518861-23518880 None:intergenic 35.0%
CTTCCATCTCTCATTGATAT+GGG + chr5_3:23518947-23518966 Msa0806070:CDS 35.0%
ATACCCATATCAATGAGAGA+TGG - chr5_3:23518953-23518972 None:intergenic 35.0%
CTTCATTGTGGTTGAACATA+AGG - chr5_3:23519036-23519055 None:intergenic 35.0%
!!! CAAATGTTTTTAGAGAAGCC+AGG - chr5_3:23519093-23519112 None:intergenic 35.0%
TTAGTCATTGAATCTTGCTC+AGG + chr5_3:23519256-23519275 Msa0806070:intron 35.0%
AAGTCATATGTTGTAAAGCC+GGG - chr5_3:23519297-23519316 None:intergenic 35.0%
GAAGTCATATGTTGTAAAGC+CGG - chr5_3:23519298-23519317 None:intergenic 35.0%
TATGACTTCCCTTCAATCAT+TGG + chr5_3:23519309-23519328 Msa0806070:intron 35.0%
ACTCAATGCCAATGATTGAA+GGG - chr5_3:23519320-23519339 None:intergenic 35.0%
!! CCTTGAACATGAGCTATTTT+TGG - chr5_3:23519538-23519557 None:intergenic 35.0%
CCAAAAATAGCTCATGTTCA+AGG + chr5_3:23519535-23519554 Msa0806070:intron 35.0%
TATTATACTCACGTGAGAAG+TGG + chr5_3:23519693-23519712 Msa0806070:intron 35.0%
!!! GTATAATTTTGAAGTGAGGG+TGG + chr5_3:23519728-23519747 Msa0806070:intron 35.0%
!! GGTGGGTGTCATTTAAATTT+AGG + chr5_3:23519785-23519804 Msa0806070:intron 35.0%
!!! TCCTGTGTTTTTTTTTCCAC+AGG + chr5_3:23519839-23519858 Msa0806070:intron 35.0%
ACCTGTGGAAAAAAAAACAC+AGG - chr5_3:23519843-23519862 None:intergenic 35.0%
!!! TGTTTTTTTTTCCACAGGTC+TGG + chr5_3:23519844-23519863 Msa0806070:intron 35.0%
! AACCCAGTTGTATATTTTCC+CGG - chr5_3:23519956-23519975 None:intergenic 35.0%
AGGCGATATAATGCTACAAT+TGG - chr5_3:23519995-23520014 None:intergenic 35.0%
!! TAATCATAGTGGGTACTAGT+TGG + chr5_3:23520357-23520376 Msa0806070:intron 35.0%
! TAGAGGCAGATGAACTTTTT+GGG - chr5_3:23520397-23520416 None:intergenic 35.0%
! ATAGAGGCAGATGAACTTTT+TGG - chr5_3:23520398-23520417 None:intergenic 35.0%
GCATGAACATTAACGAATAG+AGG - chr5_3:23520414-23520433 None:intergenic 35.0%
CTTGAGAATAGATTTAGCTG+TGG + chr5_3:23520640-23520659 Msa0806070:intron 35.0%
!!! TTTTACTTTTTTTCCCTGGG+GGG - chr5_3:23520864-23520883 None:intergenic 35.0%
TTGAATGGGGTATATTGACA+GGG - chr5_3:23520921-23520940 None:intergenic 35.0%
ATGCACAAGATAGTTGAATG+GGG - chr5_3:23520934-23520953 None:intergenic 35.0%
CAATGCACAAGATAGTTGAA+TGG - chr5_3:23520936-23520955 None:intergenic 35.0%
!!! TATGACAATTCTGTTTTGGC+AGG + chr5_3:23521038-23521057 Msa0806070:intron 35.0%
!!! TGTTTTGGCAGGAGTAAAAT+GGG + chr5_3:23521049-23521068 Msa0806070:intron 35.0%
AGTAAAATGGGTAATCAGAG+TGG + chr5_3:23521061-23521080 Msa0806070:CDS 35.0%
AGATCAAATTCTGTTGCATC+CGG + chr5_3:23521096-23521115 Msa0806070:CDS 35.0%
AGGCTTAAGTGTATTCTCTT+AGG + chr5_3:23521161-23521180 Msa0806070:exon 35.0%
GGCTTAAGTGTATTCTCTTA+GGG + chr5_3:23521162-23521181 Msa0806070:exon 35.0%
TTAAGTGTATTCTCTTAGGG+AGG + chr5_3:23521165-23521184 Msa0806070:exon 35.0%
CACAACGTACATAAGGATTC+TGG - chr5_3:23518161-23518180 None:intergenic 40.0%
CAGAATCCTTATGTACGTTG+TGG + chr5_3:23518159-23518178 Msa0806070:CDS 40.0%
! CCAGACTATTAGGCAACTTT+TGG - chr5_3:23518223-23518242 None:intergenic 40.0%
CCAAAAGTTGCCTAATAGTC+TGG + chr5_3:23518220-23518239 Msa0806070:CDS 40.0%
ATGCCAACATTCTTACATGC+GGG - chr5_3:23518307-23518326 None:intergenic 40.0%
GATGCCAACATTCTTACATG+CGG - chr5_3:23518308-23518327 None:intergenic 40.0%
! GCTTGACATATTAGCTTCCT+TGG - chr5_3:23518459-23518478 None:intergenic 40.0%
TAATATGTCAAGCAAGTGCG+TGG + chr5_3:23518466-23518485 Msa0806070:CDS 40.0%
CAACTTCGAAGTCTGTATGT+TGG + chr5_3:23518581-23518600 Msa0806070:CDS 40.0%
TAGGACTAGACATCCATTCT+GGG - chr5_3:23518628-23518647 None:intergenic 40.0%
GTTTACACCTTCCACATTTG+GGG + chr5_3:23518693-23518712 Msa0806070:intron 40.0%
CTTGATAAGGCTATCAGGTT+TGG - chr5_3:23518848-23518867 None:intergenic 40.0%
!! CAAACCTGATAGCCTTATCA+AGG + chr5_3:23518846-23518865 Msa0806070:CDS 40.0%
AAAGGCAAGCCCAACAATTT+AGG - chr5_3:23518926-23518945 None:intergenic 40.0%
TCAATGAGAGATGGAAGGAA+AGG - chr5_3:23518944-23518963 None:intergenic 40.0%
CCATATCAATGAGAGATGGA+AGG - chr5_3:23518949-23518968 None:intergenic 40.0%
CCTTCCATCTCTCATTGATA+TGG + chr5_3:23518946-23518965 Msa0806070:CDS 40.0%
! AGCAAGTTAGCTCTTCATTG+TGG - chr5_3:23519048-23519067 None:intergenic 40.0%
!! AGCTAACTTGCTTTCCAGAT+GGG + chr5_3:23519056-23519075 Msa0806070:CDS 40.0%
CTTCATCTGTCAATGACTTC+AGG - chr5_3:23519207-23519226 None:intergenic 40.0%
GAATCTTGCTCAGGAATAGA+AGG + chr5_3:23519265-23519284 Msa0806070:intron 40.0%
AGGAATAGAAGGTTTACACC+CGG + chr5_3:23519276-23519295 Msa0806070:intron 40.0%
CACTCAATGCCAATGATTGA+AGG - chr5_3:23519321-23519340 None:intergenic 40.0%
ATTTGTGGTTGCAGTGAGTT+AGG + chr5_3:23519478-23519497 Msa0806070:intron 40.0%
TTTGTGGTTGCAGTGAGTTA+GGG + chr5_3:23519479-23519498 Msa0806070:intron 40.0%
GAACGATGTAAAGAGAACAC+AGG + chr5_3:23519502-23519521 Msa0806070:intron 40.0%
ATGTAAAGAGAACACAGGTG+AGG + chr5_3:23519507-23519526 Msa0806070:intron 40.0%
GAAAACAGAGGAATGTTCTG+CGG + chr5_3:23519568-23519587 Msa0806070:intron 40.0%
! GAGGATTTTATTGTGCAGCT+TGG + chr5_3:23519599-23519618 Msa0806070:intron 40.0%
AAATCTAAACACCAGACCTG+TGG - chr5_3:23519858-23519877 None:intergenic 40.0%
! TGTCATTTCCTTGTTCAACC+CGG + chr5_3:23519934-23519953 Msa0806070:intron 40.0%
!! ATATTTTCCCGGGTTGAACA+AGG - chr5_3:23519945-23519964 None:intergenic 40.0%
AACCCGGGAAAATATACAAC+TGG + chr5_3:23519950-23519969 Msa0806070:intron 40.0%
ACCCGGGAAAATATACAACT+GGG + chr5_3:23519951-23519970 Msa0806070:intron 40.0%
! ACCCAGTTGTATATTTTCCC+GGG - chr5_3:23519955-23519974 None:intergenic 40.0%
TATAATGCTACAATTGGCGC+TGG - chr5_3:23519989-23520008 None:intergenic 40.0%
TACCCACGTAAGCAAAGAAA+AGG - chr5_3:23520015-23520034 None:intergenic 40.0%
AATTTCGCACGAAGTTGTTG+AGG - chr5_3:23520326-23520345 None:intergenic 40.0%
TTGAATGTCCCAAGTAAGAG+AGG + chr5_3:23520494-23520513 Msa0806070:intron 40.0%
CTCCAATACCTCTCTTACTT+GGG - chr5_3:23520505-23520524 None:intergenic 40.0%
GCTCACAGGATTGCTAAATA+AGG - chr5_3:23520539-23520558 None:intergenic 40.0%
TAGATTTAGCTGTGGGAATG+TGG + chr5_3:23520648-23520667 Msa0806070:intron 40.0%
!!! AGTTTTTTAGGCGTAATCGC+TGG + chr5_3:23520806-23520825 Msa0806070:intron 40.0%
AACATGTTAACTCAGTCTCG+CGG - chr5_3:23520891-23520910 None:intergenic 40.0%
GTTGAATGGGGTATATTGAC+AGG - chr5_3:23520922-23520941 None:intergenic 40.0%
!!! CTGTTTTGGCAGGAGTAAAA+TGG + chr5_3:23521048-23521067 Msa0806070:intron 40.0%
TCAATACATGGGGTGAGAGT+TGG - chr5_3:23518022-23518041 None:intergenic 45.0%
TGCCAACATTCTTACATGCG+GGG - chr5_3:23518306-23518325 None:intergenic 45.0%
CTTCGAAGTTGCTGAGTTTG+AGG - chr5_3:23518572-23518591 None:intergenic 45.0%
CTTCGAAGTCTGTATGTTGG+AGG + chr5_3:23518584-23518603 Msa0806070:CDS 45.0%
CTGTATGTTGGAGGATACAC+CGG + chr5_3:23518593-23518612 Msa0806070:CDS 45.0%
! AGACATCCATTCTGGGAAAC+AGG - chr5_3:23518621-23518640 None:intergenic 45.0%
GTAGGACTAGACATCCATTC+TGG - chr5_3:23518629-23518648 None:intergenic 45.0%
GTAGTTTCCCCAAATGTGGA+AGG - chr5_3:23518703-23518722 None:intergenic 45.0%
GATGGTAGTTTCCCCAAATG+TGG - chr5_3:23518707-23518726 None:intergenic 45.0%
ACCTCCTGCAACTCCATTAT+GGG - chr5_3:23518789-23518808 None:intergenic 45.0%
AACCTCCTGCAACTCCATTA+TGG - chr5_3:23518790-23518809 None:intergenic 45.0%
CATCCCTTGATAAGGCTATC+AGG - chr5_3:23518853-23518872 None:intergenic 45.0%
!! GAGCTAACTTGCTTTCCAGA+TGG + chr5_3:23519055-23519074 Msa0806070:CDS 45.0%
AGGTTTCTTAGCATCCCATC+TGG - chr5_3:23519073-23519092 None:intergenic 45.0%
AGATGGGATGCTAAGAAACC+TGG + chr5_3:23519072-23519091 Msa0806070:CDS 45.0%
GGATGGGAATTGCTCAAGTT+TGG - chr5_3:23519131-23519150 None:intergenic 45.0%
GTTTCCTAACCAGTCAGGTA+GGG - chr5_3:23519362-23519381 None:intergenic 45.0%
CTTAGGTTTCCTAACCAGTC+AGG - chr5_3:23519367-23519386 None:intergenic 45.0%
!! GTAAGGCATTGAATGCTCGT+CGG - chr5_3:23519439-23519458 None:intergenic 45.0%
ACGAGCATTCAATGCCTTAC+TGG + chr5_3:23519439-23519458 Msa0806070:intron 45.0%
TTGTGGTTGCAGTGAGTTAG+GGG + chr5_3:23519480-23519499 Msa0806070:intron 45.0%
AAGAGAACACAGGTGAGGAT+TGG + chr5_3:23519512-23519531 Msa0806070:intron 45.0%
TATACTCACGTGAGAAGTGG+TGG + chr5_3:23519696-23519715 Msa0806070:intron 45.0%
ATACTCACGTGAGAAGTGGT+GGG + chr5_3:23519697-23519716 Msa0806070:intron 45.0%
GTCATTTCCTTGTTCAACCC+GGG + chr5_3:23519935-23519954 Msa0806070:intron 45.0%
! TCGCCTTTTCTTTGCTTACG+TGG + chr5_3:23520009-23520028 Msa0806070:intron 45.0%
! CGCCTTTTCTTTGCTTACGT+GGG + chr5_3:23520010-23520029 Msa0806070:intron 45.0%
!!! GGCAGATGAACTTTTTGGGT+CGG - chr5_3:23520393-23520412 None:intergenic 45.0%
! GTCCCAAGTAAGAGAGGTAT+TGG + chr5_3:23520500-23520519 Msa0806070:intron 45.0%
GCTCCAATACCTCTCTTACT+TGG - chr5_3:23520506-23520525 None:intergenic 45.0%
! ACTACTGATTGGATGCTCAC+AGG - chr5_3:23520553-23520572 None:intergenic 45.0%
GAAAGTAGGGCTGCTATAGA+TGG + chr5_3:23520705-23520724 Msa0806070:intron 45.0%
ATGGGTAATCAGAGTGGACA+AGG + chr5_3:23521067-23521086 Msa0806070:CDS 45.0%
GACATCCTCTTGTTCAAGAC+CGG - chr5_3:23521118-23521137 None:intergenic 45.0%
!! ATAATAATTCTAATAAAATA+GGG - chr5_3:23521286-23521305 None:intergenic 5.0%
CTCGAAGTCAAGCACTCGTA+AGG - chr5_3:23518054-23518073 None:intergenic 50.0%
GCCAACATTCTTACATGCGG+GGG - chr5_3:23518305-23518324 None:intergenic 50.0%
! CCATTCTGGGAAACAGGAAC+CGG - chr5_3:23518615-23518634 None:intergenic 50.0%
! TCGTCCCATAATGGAGTTGC+AGG + chr5_3:23518782-23518801 Msa0806070:CDS 50.0%
! TCCCATAATGGAGTTGCAGG+AGG + chr5_3:23518785-23518804 Msa0806070:CDS 50.0%
TCAGGTAGGGATGCTAGGTT+TGG - chr5_3:23519349-23519368 None:intergenic 50.0%
GGTTTCCTAACCAGTCAGGT+AGG - chr5_3:23519363-23519382 None:intergenic 50.0%
AACAGAGGAATGTTCTGCGG+AGG + chr5_3:23519571-23519590 Msa0806070:intron 50.0%
!!! TTTTTTTCCCTGGGGGGTTG+GGG - chr5_3:23520858-23520877 None:intergenic 50.0%
!!! CTTTTTTTCCCTGGGGGGTT+GGG - chr5_3:23520859-23520878 None:intergenic 50.0%
!!! ACTTTTTTTCCCTGGGGGGT+TGG - chr5_3:23520860-23520879 None:intergenic 50.0%
TGCATCCGGTCTTGAACAAG+AGG + chr5_3:23521110-23521129 Msa0806070:CDS 50.0%
GCCCCCGCATGTAAGAATGT+TGG + chr5_3:23518301-23518320 Msa0806070:CDS 55.0%
CCGGTTCCTGTTTCCCAGAA+TGG + chr5_3:23518612-23518631 Msa0806070:intron 55.0%
GACGAGGAGTCGTCCCATAA+TGG + chr5_3:23518773-23518792 Msa0806070:CDS 55.0%
ACCTAGCATCCCTACCTGAC+TGG + chr5_3:23519350-23519369 Msa0806070:intron 55.0%
ACCAGTCAGGTAGGGATGCT+AGG - chr5_3:23519354-23519373 None:intergenic 55.0%
! GCATCCCTACCTGACTGGTT+AGG + chr5_3:23519355-23519374 Msa0806070:intron 55.0%
GTCGGAAGACACGTCAGCTT+TGG - chr5_3:23519421-23519440 None:intergenic 55.0%
AGAGGAATGTTCTGCGGAGG+AGG + chr5_3:23519574-23519593 Msa0806070:intron 55.0%
!!! TTTTTTCCCTGGGGGGTTGG+GGG - chr5_3:23520857-23520876 None:intergenic 55.0%
GGAATGTTCTGCGGAGGAGG+AGG + chr5_3:23519577-23519596 Msa0806070:intron 60.0%
ATGTTCTGCGGAGGAGGAGG+AGG + chr5_3:23519580-23519599 Msa0806070:intron 60.0%
TAGTAATCCCCCAACCCCCC+AGG + chr5_3:23520847-23520866 Msa0806070:intron 60.0%
AGTAATCCCCCAACCCCCCA+GGG + chr5_3:23520848-23520867 Msa0806070:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 23517948 23521396 23517948 ID=Msa0806070;Name=Msa0806070
chr5_3 mRNA 23517948 23521396 23517948 ID=Msa0806070-mRNA-1;Parent=Msa0806070;Name=Msa0806070-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0.16|1|0|0.16|6|228|393
chr5_3 exon 23517948 23518084 23517948 ID=Msa0806070-mRNA-1:exon:9245;Parent=Msa0806070-mRNA-1
chr5_3 exon 23518121 23518368 23518121 ID=Msa0806070-mRNA-1:exon:9246;Parent=Msa0806070-mRNA-1
chr5_3 exon 23518384 23518614 23518384 ID=Msa0806070-mRNA-1:exon:9247;Parent=Msa0806070-mRNA-1
chr5_3 exon 23518744 23518968 23518744 ID=Msa0806070-mRNA-1:exon:9248;Parent=Msa0806070-mRNA-1
chr5_3 exon 23518978 23519209 23518978 ID=Msa0806070-mRNA-1:exon:9249;Parent=Msa0806070-mRNA-1
chr5_3 exon 23521060 23521396 23521060 ID=Msa0806070-mRNA-1:exon:9250;Parent=Msa0806070-mRNA-1
chr5_3 CDS 23517948 23518084 23517948 ID=Msa0806070-mRNA-1:cds;Parent=Msa0806070-mRNA-1
chr5_3 CDS 23518121 23518368 23518121 ID=Msa0806070-mRNA-1:cds;Parent=Msa0806070-mRNA-1
chr5_3 CDS 23518384 23518614 23518384 ID=Msa0806070-mRNA-1:cds;Parent=Msa0806070-mRNA-1
chr5_3 CDS 23518744 23518968 23518744 ID=Msa0806070-mRNA-1:cds;Parent=Msa0806070-mRNA-1
chr5_3 CDS 23518978 23519209 23518978 ID=Msa0806070-mRNA-1:cds;Parent=Msa0806070-mRNA-1
chr5_3 CDS 23521060 23521168 23521060 ID=Msa0806070-mRNA-1:cds;Parent=Msa0806070-mRNA-1
chr5_3 three_prime_UTR 23521169 23521396 23521169 ID=Msa0806070-mRNA-1:three_prime_utr;Parent=Msa0806070-mRNA-1
Gene Sequence

>Msa0806070

ATGTTAGAGGAAGTTGGTTCAATCACGTTACACTGTGTTGAATCATTAAAAACATGTATAAATTGTGTTGACCAACTCTCACCCCATGTATTGAGATGTTATTCCTTACGAGTGCTTGACTTCGAGAGCAAAGAAAAGTACTTGAATCTTTCTTATGGATATTTCAAAACTCTTCCAGAATCCTTATGTACGTTGTGGAATTTGCAGATTTTAAAGTTAGACTATTGTCGAGATCTCCAAAAGTTGCCTAATAGTCTGGTACACTTGAAATCTCTACAACATCTATATTTGAGACGCTGCAATTCTCTATCAAGCTTGCCCCCGCATGTAAGAATGTTGGCATCCTTGAAAATTTTAACACTATATGTAGTTGGCATGAAAAAAGAGTTAGGACAAATGAACCTTCAAGGAGACCTTTACATTAAGCACTTGGAGAGAGTTAAAAGTGTAATGAATGCCAAGGAAGCTAATATGTCAAGCAAGTGCGTGGACAATTTGAAATTGTCATGGGATAGAAATGAAGTGTCACAAGTACAGGAAAATGTTGAGGAGATTCTTGAAGTGCTTCAACCTCAAACTCAGCAACTTCGAAGTCTGTATGTTGGAGGATACACCGTATCTAGTATGAGTCATGTAAAATACTTAGACGAGGAGTCGTCCCATAATGGAGTTGCAGGAGGTTTCATACGTCTAGAATGTCTGATTCTAGAGAAGCTGCCAAACCTGATAGCCTTATCAAGGGATGATAGAGAAAACATGCTTCCACACCTTTCTAAATTTCAAATTACTGAATGTCCTAAATTGTTGGGCTTGCCTTTCCTTCCATCTCTCATTGATATGGGAAAATGCAACACGGGTTTACTAACTTCAATTCATAAACTTGTTAGTCTTGAATCCCTTATGTTCAACCACAATGAAGAGCTAACTTGCTTTCCAGATGGGATGCTAAGAAACCTGGCTTCTCTAAAAACATTTGACATTTGTGGGATTTCCAAACTTGAGCAATTCCCATCCGAAATCTTTAACATTAACACCATCCAAGAAATATATATCAAAAAATGTGATAACCTGAAGAGTAAAATGGGTAATCAGAGTGGACAAGGACATGAAGATCAAATTCTGTTGCATCCGGTCTTGAACAAGAGGATGTCCTACATTGCTTTTTATTCATTCAAGGCTTAA
Protein sequence

>Msa0806070

MLEEVGSITLHCVESLKTCINCVDQLSPHVLRCYSLRVLDFESKEKYLNLSYGYFKTLPESLCTLWNLQILKLDYCRDLQKLPNSLVHLKSLQHLYLRRCNSLSSLPPHVRMLASLKILTLYVVGMKKELGQMNLQGDLYIKHLERVKSVMNAKEANMSSKCVDNLKLSWDRNEVSQVQENVEEILEVLQPQTQQLRSLYVGGYTVSSMSHVKYLDEESSHNGVAGGFIRLECLILEKLPNLIALSRDDRENMLPHLSKFQITECPKLLGLPFLPSLIDMGKCNTGLLTSIHKLVSLESLMFNHNEELTCFPDGMLRNLASLKTFDICGISKLEQFPSEIFNINTIQEIYIKKCDNLKSKMGNQSGQGHEDQILLHPVLNKRMSYIAFYSFKA*