AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0845870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0845870 MtrunA17_Chr5g0417571 88.927 289 29 2 1 287 1 288 5.56e-173 481
Msa0845870 MtrunA17_Chr5g0417551 72.165 291 61 5 1 286 1 276 3.30e-134 384
Msa0845870 MtrunA17_Chr5g0417601 68.750 288 77 3 1 283 1 280 3.67e-126 364
Msa0845870 MtrunA17_Chr5g0417561 67.698 291 66 7 1 286 1 268 5.58e-122 353
Msa0845870 MtrunA17_Chr4g0042481 35.561 374 114 15 2 283 4 342 1.40e-49 170
Msa0845870 MtrunA17_Chr7g0217581 38.258 264 116 6 4 243 103 343 2.68e-43 155
Msa0845870 MtrunA17_Chr6g0453321 38.333 240 115 7 6 220 8 239 1.42e-39 144
Msa0845870 MtrunA17_Chr3g0092111 38.462 234 114 7 7 216 9 236 2.39e-36 135
Msa0845870 MtrunA17_Chr7g0254161 54.310 116 32 3 126 220 90 205 7.99e-35 130
Msa0845870 MtrunA17_Chr6g0460731 54.369 103 43 2 97 195 53 155 7.23e-33 124
Msa0845870 MtrunA17_Chr8g0355261 67.568 74 24 0 117 190 98 171 4.03e-32 123
Msa0845870 MtrunA17_Chr6g0460721 53.125 96 42 1 98 190 47 142 5.40e-30 115
Msa0845870 MtrunA17_Chr2g0283811 70.000 60 18 0 131 190 41 100 1.93e-29 109
Msa0845870 MtrunA17_Chr2g0326001 63.636 66 23 1 126 190 28 93 2.69e-25 102
Msa0845870 MtrunA17_Chr3g0127201 57.143 70 30 0 124 193 14 83 4.58e-25 101
Msa0845870 MtrunA17_Chr8g0374111 63.934 61 22 0 129 189 21 81 2.29e-24 98.2
Msa0845870 MtrunA17_Chr2g0328021 55.714 70 31 0 121 190 46 115 6.15e-24 98.2
Msa0845870 MtrunA17_Chr8g0341671 62.500 64 23 1 126 188 8 71 6.44e-24 96.7
Msa0845870 MtrunA17_Chr4g0010151 48.684 76 39 0 115 190 60 135 1.46e-23 98.6
Msa0845870 MtrunA17_Chr8g0346551 61.017 59 23 0 132 190 19 77 1.86e-23 96.7
Msa0845870 MtrunA17_Chr4g0030051 61.538 65 24 1 126 189 29 93 2.36e-23 96.7
Msa0845870 MtrunA17_Chr8g0374251 57.333 75 31 1 135 208 66 140 3.13e-23 97.4
Msa0845870 MtrunA17_Chr3g0126201 57.746 71 28 2 122 190 34 104 7.86e-23 96.3
Msa0845870 MtrunA17_Chr4g0061761 67.925 53 17 0 135 187 38 90 1.09e-22 95.5
Msa0845870 MtrunA17_Chr1g0188851 59.375 64 25 1 127 189 32 95 1.32e-22 94.7
Msa0845870 MtrunA17_Chr7g0223311 70.588 51 15 0 139 189 79 129 2.02e-22 95.5
Msa0845870 MtrunA17_Chr8g0367161 54.167 72 33 0 119 190 14 85 2.46e-22 94.7
Msa0845870 MtrunA17_Chr1g0177251 63.333 60 21 1 132 190 6 65 2.67e-22 94.0
Msa0845870 MtrunA17_Chr2g0282051 47.500 80 42 0 112 191 21 100 3.31e-22 93.6
Msa0845870 MtrunA17_Chr4g0047761 67.925 53 17 0 139 191 74 126 3.96e-22 94.0
Msa0845870 MtrunA17_Chr4g0031411 61.404 57 22 0 133 189 71 127 5.11e-22 94.0
Msa0845870 MtrunA17_Chr4g0047001 69.231 52 16 0 139 190 57 108 5.27e-22 94.7
Msa0845870 MtrunA17_Chr2g0308011 68.000 50 16 0 136 185 36 85 6.80e-22 91.3
Msa0845870 MtrunA17_Chr5g0411951 65.455 55 19 0 133 187 39 93 1.07e-21 93.2
Msa0845870 MtrunA17_Chr7g0237921 63.462 52 19 0 139 190 78 129 1.27e-20 90.1
Msa0845870 MtrunA17_Chr2g0282511 66.000 50 17 0 136 185 44 93 1.99e-20 89.0
Msa0845870 MtrunA17_Chr3g0117601 59.677 62 24 1 127 187 50 111 2.79e-20 89.0
Msa0845870 MtrunA17_Chr2g0282441 68.627 51 16 0 139 189 27 77 3.44e-20 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0845870 AT1G69570.1 44.048 168 62 6 131 272 126 287 7.58e-39 140
Msa0845870 AT1G26790.1 37.443 219 110 7 84 281 59 271 1.34e-36 134
Msa0845870 AT5G39660.3 36.546 249 120 7 2 222 1 239 2.67e-36 135
Msa0845870 AT5G39660.1 36.546 249 120 7 2 222 1 239 2.67e-36 135
Msa0845870 AT5G39660.2 36.546 249 120 7 2 222 1 239 2.67e-36 135
Msa0845870 AT5G62430.1 46.203 158 60 7 122 261 38 188 2.81e-35 129
Msa0845870 AT3G47500.1 54.054 111 38 1 86 196 72 169 5.27e-33 126
Msa0845870 AT1G29160.1 65.217 69 21 1 122 190 50 115 1.85e-31 115
Msa0845870 AT2G34140.1 40.741 135 66 4 85 208 2 133 7.12e-31 114
Msa0845870 AT5G60200.1 61.538 65 25 0 130 194 48 112 5.85e-25 100
Msa0845870 AT1G51700.1 60.000 65 25 1 125 188 20 84 1.66e-24 98.2
Msa0845870 AT2G37590.1 50.000 74 37 0 117 190 69 142 3.30e-24 100
Msa0845870 AT4G24060.1 59.091 66 27 0 125 190 41 106 4.55e-24 100
Msa0845870 AT3G45610.1 61.538 65 25 0 130 194 33 97 5.66e-24 97.8
Msa0845870 AT3G61850.2 55.714 70 31 0 125 194 50 119 2.39e-23 97.1
Msa0845870 AT3G61850.3 55.714 70 31 0 125 194 50 119 2.39e-23 97.1
Msa0845870 AT3G55370.3 41.237 97 56 1 98 194 83 178 2.60e-23 98.6
Msa0845870 AT3G61850.1 55.714 70 31 0 125 194 62 131 2.66e-23 97.1
Msa0845870 AT3G55370.1 41.237 97 56 1 98 194 38 133 2.88e-23 97.4
Msa0845870 AT3G61850.5 55.714 70 31 0 125 194 50 119 3.64e-23 97.1
Msa0845870 AT2G28810.2 43.182 88 48 1 101 188 43 128 3.71e-23 97.4
Msa0845870 AT3G61850.4 55.714 70 31 0 125 194 62 131 3.73e-23 97.4
Msa0845870 AT3G55370.2 41.237 97 56 1 98 194 38 133 4.13e-23 97.8
Msa0845870 AT2G28810.1 43.182 88 48 1 101 188 60 145 4.83e-23 97.4
Msa0845870 AT5G60850.1 63.333 60 22 0 135 194 49 108 7.59e-23 96.3
Msa0845870 AT3G50410.1 69.231 52 16 0 137 188 30 81 9.83e-23 94.7
Msa0845870 AT3G21270.1 70.588 51 15 0 139 189 31 81 2.14e-22 92.8
Msa0845870 AT1G07640.1 68.627 51 16 0 139 189 23 73 2.52e-22 94.0
Msa0845870 AT1G07640.2 54.286 70 26 1 120 189 66 129 3.04e-22 94.7
Msa0845870 AT1G07640.3 54.286 70 26 1 120 189 74 137 3.44e-22 94.7
Msa0845870 AT2G28510.1 66.071 56 19 0 139 194 50 105 5.41e-22 93.6
Msa0845870 AT5G66940.1 44.211 95 40 1 95 189 3 84 6.93e-22 91.7
Msa0845870 AT1G64620.1 56.250 64 27 1 122 185 35 97 1.67e-21 93.2
Msa0845870 AT3G52440.1 50.588 85 37 1 139 223 27 106 2.38e-21 90.9
Msa0845870 AT1G28310.1 64.815 54 19 0 137 190 27 80 2.92e-21 92.0
Msa0845870 AT5G65590.1 63.793 58 21 0 135 192 39 96 3.39e-21 91.7
Msa0845870 AT1G28310.2 64.815 54 19 0 137 190 51 104 3.68e-21 92.0
Msa0845870 AT3G52440.2 61.404 57 21 1 135 190 41 97 3.85e-21 90.9
Msa0845870 AT1G47655.1 48.718 78 37 1 108 185 3 77 4.40e-21 89.4
Msa0845870 AT4G38000.1 64.286 56 19 1 136 190 40 95 7.86e-21 89.4
Msa0845870 AT4G00940.1 57.627 59 25 0 132 190 63 121 1.13e-20 90.1
Msa0845870 AT4G00940.3 57.627 59 25 0 132 190 63 121 1.13e-20 90.1
Msa0845870 AT4G00940.2 57.627 59 25 0 132 190 63 121 1.13e-20 90.1
Msa0845870 AT5G02460.1 58.621 58 24 0 133 190 91 148 1.15e-20 91.3
Msa0845870 AT1G21340.1 66.000 50 17 0 139 188 40 89 1.75e-20 89.0
Msa0845870 AT5G62940.1 40.984 122 48 2 139 236 75 196 1.95e-20 90.5
Msa0845870 AT2G46590.1 60.377 53 21 0 132 184 63 115 4.80e-20 89.0
Msa0845870 AT2G46590.2 60.377 53 21 0 132 184 75 127 5.26e-20 89.4
Msa0845870 AT4G21050.1 54.688 64 29 0 125 188 12 75 3.54e-19 84.0
Msa0845870 AT4G21040.1 63.462 52 19 0 139 190 27 78 9.19e-18 80.9
Msa0845870 AT4G21080.1 63.462 52 19 0 139 190 27 78 4.02e-17 79.3

Find 54 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACCAAATAACTAACTTTA+AGG 0.142835 5_4:+25287146 Msa0845870:exon
TTTCCTTAAAGTTAGTTATT+TGG 0.224729 5_4:-25287149 None:intergenic
CCACCATAGAATGCTATATT+TGG 0.230148 5_4:-25287074 None:intergenic
ACAACTTGGTCTGGTTTCTT+TGG 0.248772 5_4:-25286681 None:intergenic
CTCCAGAAGTCCAGTATCTT+TGG 0.293736 5_4:-25286794 None:intergenic
TGCCAAAGATACTGGACTTC+TGG 0.300675 5_4:+25286792 Msa0845870:CDS
TTCCATGAGATGATTTGATT+TGG 0.304050 5_4:+25287271 Msa0845870:three_prime_UTR
AATGCTATATTTGGATAGAT+TGG 0.337846 5_4:-25287065 None:intergenic
ATGAGTACTGTCTTGACATT+TGG 0.342898 5_4:+25286906 Msa0845870:CDS
TATCCAAATATAGCATTCTA+TGG 0.352424 5_4:+25287071 Msa0845870:CDS
AGTGAAGTTGAAGACATAAT+AGG 0.361504 5_4:-25286939 None:intergenic
GAGATGATTTGATTTGGAAT+AGG 0.387321 5_4:+25287277 Msa0845870:three_prime_UTR
ACATGATGCAAAGAGATATA+AGG 0.388068 5_4:+25285828 Msa0845870:CDS
ATTCTGAATCCTCTGTGGTT+TGG 0.395071 5_4:-25286542 None:intergenic
AGCATTCTATGGTGGTTGTT+TGG 0.399605 5_4:+25287082 Msa0845870:CDS
GGACATGGAACAACTTGGTC+TGG 0.406395 5_4:-25286690 None:intergenic
GTGGTTGTTTGGTACCATCT+TGG 0.412555 5_4:+25287093 Msa0845870:CDS
ATCTTTCGTTCAAACAATGA+TGG 0.431104 5_4:+25287193 Msa0845870:three_prime_UTR
ACCACGAGAAATATGCTTAT+AGG 0.437951 5_4:+25286819 Msa0845870:CDS
ACTGTGTTGAGATGGATTGT+AGG 0.456181 5_4:-25287118 None:intergenic
CTAACGCTGCAGCTTTATCA+AGG 0.467712 5_4:+25287239 Msa0845870:three_prime_UTR
GTTGAAGAATTCACTTCAAG+TGG 0.481761 5_4:-25285802 None:intergenic
TAAGGAAACATTCAAGAGAT+GGG 0.488389 5_4:+25287164 Msa0845870:three_prime_UTR
AAATCTTCATGAGCAATAGT+AGG 0.488963 5_4:-25285739 None:intergenic
TTCCAAATCAAATCATCTCA+TGG 0.489753 5_4:-25287273 None:intergenic
TACTGTCTTGACATTTGGAT+CGG 0.502009 5_4:+25286911 Msa0845870:CDS
AAAAGAACTCATCGAGGATG+AGG 0.512018 5_4:+25286512 Msa0845870:CDS
TTAAGGAAACATTCAAGAGA+TGG 0.512653 5_4:+25287163 Msa0845870:three_prime_UTR
GACGTCTCTGTTTCAGATGA+GGG 0.514212 5_4:-25286603 None:intergenic
TTCATTAGACTGTGTTGAGA+TGG 0.528897 5_4:-25287126 None:intergenic
AGAAATATGCTTATAGGCGC+AGG 0.536173 5_4:+25286825 Msa0845870:CDS
ATATTTGGATAGATTGGCAC+AGG 0.538462 5_4:-25287059 None:intergenic
TAACACATAGCAGGATTCCA+TGG 0.544411 5_4:-25287035 None:intergenic
CAATGATGGAGGACTTTCCA+AGG 0.547224 5_4:+25287207 Msa0845870:three_prime_UTR
AGACGTCTCTGTTTCAGATG+AGG 0.571871 5_4:-25286604 None:intergenic
AAGAAGAGATTCTGTTCTTG+CGG 0.572733 5_4:-25286851 None:intergenic
ATCTTGGACATGGAACAACT+TGG 0.574876 5_4:-25286695 None:intergenic
TCAATAAGGCTTGAAGATGT+CGG 0.575632 5_4:-25286567 None:intergenic
GCCTATAAGCATATTTCTCG+TGG 0.581874 5_4:-25286820 None:intergenic
GGCACAGGATAACACATAGC+AGG 0.586834 5_4:-25287044 None:intergenic
GGATTCCATGGAAACTGTTG+AGG 0.594593 5_4:-25287023 None:intergenic
AATAGTAGGAGTAAATTCTG+AGG 0.595274 5_4:-25285725 None:intergenic
AGCGTTAGAAGCAAAACCCT+TGG 0.603793 5_4:-25287224 None:intergenic
ATGGATTGTAGGCTCCAAGA+TGG 0.604901 5_4:-25287107 None:intergenic
AAGGAAACATTCAAGAGATG+GGG 0.613145 5_4:+25287165 Msa0845870:three_prime_UTR
TTTCGTTCAAACAATGATGG+AGG 0.626558 5_4:+25287196 Msa0845870:three_prime_UTR
AATGATGGAGGACTTTCCAA+GGG 0.627563 5_4:+25287208 Msa0845870:three_prime_UTR
CGGAGATTCTGAATCCTCTG+TGG 0.629252 5_4:-25286547 None:intergenic
CAAAGATACTGGACTTCTGG+AGG 0.634659 5_4:+25286795 Msa0845870:CDS
CCAAATATAGCATTCTATGG+TGG 0.645659 5_4:+25287074 Msa0845870:CDS
ATGTCCAAGATGCAAAAGCA+CGG 0.662046 5_4:+25286707 Msa0845870:CDS
ATCAAGAAAAGAACTCATCG+AGG 0.704142 5_4:+25286506 Msa0845870:CDS
GGCTTCATTCCAAACCACAG+AGG 0.724092 5_4:+25286533 Msa0845870:CDS
ACTCATTTGATGATGATGTG+AGG 0.757465 5_4:-25286889 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATAAGAACAAATAAAT+CGG + chr5_4:25286268-25286287 Msa0845870:intron 10.0%
!! TTATATAAGAACAAATAAAT+CGG + chr5_4:25286268-25286287 Msa0845870:intron 10.0%
!!! TTCAAATTTTTCATCTTTTT+TGG + chr5_4:25285927-25285946 Msa0845870:intron 15.0%
!! AATACATGTAGTTATATTAT+CGG + chr5_4:25286357-25286376 Msa0845870:intron 15.0%
!!! TATAAGCCAATAAAATTTTA+TGG + chr5_4:25286436-25286455 Msa0845870:intron 15.0%
!!! TTCAAATTTTTCATCTTTTT+TGG + chr5_4:25285927-25285946 Msa0845870:intron 15.0%
!! AATACATGTAGTTATATTAT+CGG + chr5_4:25286357-25286376 Msa0845870:intron 15.0%
!!! TATAAGCCAATAAAATTTTA+TGG + chr5_4:25286436-25286455 Msa0845870:intron 15.0%
!!! TATGAGCCATAAAATTTTAT+TGG - chr5_4:25286445-25286464 None:intergenic 20.0%
!!! AAATTTTATGGCTCATATTT+TGG + chr5_4:25286448-25286467 Msa0845870:intron 20.0%
!!! AGTTTGTTTATCAATTTTAG+TGG - chr5_4:25286640-25286659 None:intergenic 20.0%
!! TTTCCTTAAAGTTAGTTATT+TGG - chr5_4:25287152-25287171 None:intergenic 20.0%
!!! TATGAGCCATAAAATTTTAT+TGG - chr5_4:25286445-25286464 None:intergenic 20.0%
!!! AAATTTTATGGCTCATATTT+TGG + chr5_4:25286448-25286467 Msa0845870:intron 20.0%
!!! AGTTTGTTTATCAATTTTAG+TGG - chr5_4:25286640-25286659 None:intergenic 20.0%
!! TTTCCTTAAAGTTAGTTATT+TGG - chr5_4:25287152-25287171 None:intergenic 20.0%
!! AATTCAACCATAAAGCTTTT+TGG + chr5_4:25285652-25285671 Msa0845870:CDS 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5_4:25285653-25285672 Msa0845870:CDS 25.0%
! TTCTGTTCGAAAAATATACA+CGG + chr5_4:25286030-25286049 Msa0845870:intron 25.0%
!!! TATATTTTGATCCTAACATG+AGG - chr5_4:25286140-25286159 None:intergenic 25.0%
! AAACAACAGAAAATTCATGT+TGG - chr5_4:25286246-25286265 None:intergenic 25.0%
! AACAGCTATATATCAATTTC+AGG + chr5_4:25286479-25286498 Msa0845870:intron 25.0%
! AATGCTATATTTGGATAGAT+TGG - chr5_4:25287068-25287087 None:intergenic 25.0%
! TATCCAAATATAGCATTCTA+TGG + chr5_4:25287071-25287090 Msa0845870:CDS 25.0%
! GAACCAAATAACTAACTTTA+AGG + chr5_4:25287146-25287165 Msa0845870:exon 25.0%
! TATGGTTGAATTGTTTATCA+TGG - chr5_4:25285644-25285663 None:intergenic 25.0%
!! AATTCAACCATAAAGCTTTT+TGG + chr5_4:25285652-25285671 Msa0845870:CDS 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5_4:25285653-25285672 Msa0845870:CDS 25.0%
! TTCTGTTCGAAAAATATACA+CGG + chr5_4:25286030-25286049 Msa0845870:intron 25.0%
!!! TATATTTTGATCCTAACATG+AGG - chr5_4:25286140-25286159 None:intergenic 25.0%
! AAACAACAGAAAATTCATGT+TGG - chr5_4:25286246-25286265 None:intergenic 25.0%
! AACAGCTATATATCAATTTC+AGG + chr5_4:25286479-25286498 Msa0845870:intron 25.0%
! AATGCTATATTTGGATAGAT+TGG - chr5_4:25287068-25287087 None:intergenic 25.0%
! TATCCAAATATAGCATTCTA+TGG + chr5_4:25287071-25287090 Msa0845870:CDS 25.0%
! GAACCAAATAACTAACTTTA+AGG + chr5_4:25287146-25287165 Msa0845870:exon 25.0%
AATAGTAGGAGTAAATTCTG+AGG - chr5_4:25285728-25285747 None:intergenic 30.0%
AAATCTTCATGAGCAATAGT+AGG - chr5_4:25285742-25285761 None:intergenic 30.0%
ACATGATGCAAAGAGATATA+AGG + chr5_4:25285828-25285847 Msa0845870:CDS 30.0%
TTCTTCAAATAGAATCACCA+TGG - chr5_4:25285865-25285884 None:intergenic 30.0%
AAAATATGTTGCTAGCTAGT+AGG + chr5_4:25285899-25285918 Msa0845870:intron 30.0%
! GTTGATGAAATTGTTCATCA+AGG + chr5_4:25286052-25286071 Msa0845870:intron 30.0%
! CTCTCTTTATATTTGTTGGT+AGG - chr5_4:25286206-25286225 None:intergenic 30.0%
GCATCTCTCTTTATATTTGT+TGG - chr5_4:25286210-25286229 None:intergenic 30.0%
!! AAGAGAGATGCTTTTTATCA+AGG + chr5_4:25286219-25286238 Msa0845870:intron 30.0%
! TACATGTATTGACATCATGT+TGG - chr5_4:25286347-25286366 None:intergenic 30.0%
AGTGAAGTTGAAGACATAAT+AGG - chr5_4:25286942-25286961 None:intergenic 30.0%
TCAACTTCACTTCACAAAAA+AGG + chr5_4:25286951-25286970 Msa0845870:CDS 30.0%
CAACTTCACTTCACAAAAAA+GGG + chr5_4:25286952-25286971 Msa0845870:CDS 30.0%
TTAAGGAAACATTCAAGAGA+TGG + chr5_4:25287163-25287182 Msa0845870:three_prime_UTR 30.0%
TAAGGAAACATTCAAGAGAT+GGG + chr5_4:25287164-25287183 Msa0845870:three_prime_UTR 30.0%
ATCTTTCGTTCAAACAATGA+TGG + chr5_4:25287193-25287212 Msa0845870:three_prime_UTR 30.0%
TTCCATGAGATGATTTGATT+TGG + chr5_4:25287271-25287290 Msa0845870:three_prime_UTR 30.0%
TTCCAAATCAAATCATCTCA+TGG - chr5_4:25287276-25287295 None:intergenic 30.0%
!! GAGATGATTTGATTTGGAAT+AGG + chr5_4:25287277-25287296 Msa0845870:three_prime_UTR 30.0%
AATAGTAGGAGTAAATTCTG+AGG - chr5_4:25285728-25285747 None:intergenic 30.0%
AAATCTTCATGAGCAATAGT+AGG - chr5_4:25285742-25285761 None:intergenic 30.0%
ACATGATGCAAAGAGATATA+AGG + chr5_4:25285828-25285847 Msa0845870:CDS 30.0%
TTCTTCAAATAGAATCACCA+TGG - chr5_4:25285865-25285884 None:intergenic 30.0%
AAAATATGTTGCTAGCTAGT+AGG + chr5_4:25285899-25285918 Msa0845870:intron 30.0%
! GTTGATGAAATTGTTCATCA+AGG + chr5_4:25286052-25286071 Msa0845870:intron 30.0%
! CTCTCTTTATATTTGTTGGT+AGG - chr5_4:25286206-25286225 None:intergenic 30.0%
GCATCTCTCTTTATATTTGT+TGG - chr5_4:25286210-25286229 None:intergenic 30.0%
!! AAGAGAGATGCTTTTTATCA+AGG + chr5_4:25286219-25286238 Msa0845870:intron 30.0%
! TACATGTATTGACATCATGT+TGG - chr5_4:25286347-25286366 None:intergenic 30.0%
AGTGAAGTTGAAGACATAAT+AGG - chr5_4:25286942-25286961 None:intergenic 30.0%
TCAACTTCACTTCACAAAAA+AGG + chr5_4:25286951-25286970 Msa0845870:CDS 30.0%
CAACTTCACTTCACAAAAAA+GGG + chr5_4:25286952-25286971 Msa0845870:CDS 30.0%
TTAAGGAAACATTCAAGAGA+TGG + chr5_4:25287163-25287182 Msa0845870:three_prime_UTR 30.0%
TAAGGAAACATTCAAGAGAT+GGG + chr5_4:25287164-25287183 Msa0845870:three_prime_UTR 30.0%
ATCTTTCGTTCAAACAATGA+TGG + chr5_4:25287193-25287212 Msa0845870:three_prime_UTR 30.0%
TTCCATGAGATGATTTGATT+TGG + chr5_4:25287271-25287290 Msa0845870:three_prime_UTR 30.0%
TTCCAAATCAAATCATCTCA+TGG - chr5_4:25287276-25287295 None:intergenic 30.0%
!! GAGATGATTTGATTTGGAAT+AGG + chr5_4:25287277-25287296 Msa0845870:three_prime_UTR 30.0%
GTTGAAGAATTCACTTCAAG+TGG - chr5_4:25285805-25285824 None:intergenic 35.0%
CAAATAGAATCACCATGGTA+TGG - chr5_4:25285860-25285879 None:intergenic 35.0%
TATATGAGCAACCTCATGTT+AGG + chr5_4:25286126-25286145 Msa0845870:intron 35.0%
ATAGTAACAAGAGACCTTGA+TGG - chr5_4:25286177-25286196 None:intergenic 35.0%
ATCAAGAAAAGAACTCATCG+AGG + chr5_4:25286506-25286525 Msa0845870:CDS 35.0%
TCAATAAGGCTTGAAGATGT+CGG - chr5_4:25286570-25286589 None:intergenic 35.0%
!!! GAGTTTTCAGGTTCTCAATA+AGG - chr5_4:25286584-25286603 None:intergenic 35.0%
GCAAAAATTGCCAAAGATAC+TGG + chr5_4:25286784-25286803 Msa0845870:CDS 35.0%
ACCACGAGAAATATGCTTAT+AGG + chr5_4:25286819-25286838 Msa0845870:CDS 35.0%
AAGAAGAGATTCTGTTCTTG+CGG - chr5_4:25286854-25286873 None:intergenic 35.0%
!! ACTCATTTGATGATGATGTG+AGG - chr5_4:25286892-25286911 None:intergenic 35.0%
! ATGAGTACTGTCTTGACATT+TGG + chr5_4:25286906-25286925 Msa0845870:CDS 35.0%
TACTGTCTTGACATTTGGAT+CGG + chr5_4:25286911-25286930 Msa0845870:CDS 35.0%
CTTCACAAAAAAGGGAATGT+TGG + chr5_4:25286960-25286979 Msa0845870:CDS 35.0%
ATATTTGGATAGATTGGCAC+AGG - chr5_4:25287062-25287081 None:intergenic 35.0%
CCACCATAGAATGCTATATT+TGG - chr5_4:25287077-25287096 None:intergenic 35.0%
CCAAATATAGCATTCTATGG+TGG + chr5_4:25287074-25287093 Msa0845870:CDS 35.0%
TTCATTAGACTGTGTTGAGA+TGG - chr5_4:25287129-25287148 None:intergenic 35.0%
AAGGAAACATTCAAGAGATG+GGG + chr5_4:25287165-25287184 Msa0845870:three_prime_UTR 35.0%
TTTCGTTCAAACAATGATGG+AGG + chr5_4:25287196-25287215 Msa0845870:three_prime_UTR 35.0%
GTTGAAGAATTCACTTCAAG+TGG - chr5_4:25285805-25285824 None:intergenic 35.0%
CAAATAGAATCACCATGGTA+TGG - chr5_4:25285860-25285879 None:intergenic 35.0%
TATATGAGCAACCTCATGTT+AGG + chr5_4:25286126-25286145 Msa0845870:intron 35.0%
ATAGTAACAAGAGACCTTGA+TGG - chr5_4:25286177-25286196 None:intergenic 35.0%
ATCAAGAAAAGAACTCATCG+AGG + chr5_4:25286506-25286525 Msa0845870:CDS 35.0%
TCAATAAGGCTTGAAGATGT+CGG - chr5_4:25286570-25286589 None:intergenic 35.0%
!!! GAGTTTTCAGGTTCTCAATA+AGG - chr5_4:25286584-25286603 None:intergenic 35.0%
GCAAAAATTGCCAAAGATAC+TGG + chr5_4:25286784-25286803 Msa0845870:CDS 35.0%
ACCACGAGAAATATGCTTAT+AGG + chr5_4:25286819-25286838 Msa0845870:CDS 35.0%
AAGAAGAGATTCTGTTCTTG+CGG - chr5_4:25286854-25286873 None:intergenic 35.0%
!! ACTCATTTGATGATGATGTG+AGG - chr5_4:25286892-25286911 None:intergenic 35.0%
! ATGAGTACTGTCTTGACATT+TGG + chr5_4:25286906-25286925 Msa0845870:CDS 35.0%
TACTGTCTTGACATTTGGAT+CGG + chr5_4:25286911-25286930 Msa0845870:CDS 35.0%
CTTCACAAAAAAGGGAATGT+TGG + chr5_4:25286960-25286979 Msa0845870:CDS 35.0%
ATATTTGGATAGATTGGCAC+AGG - chr5_4:25287062-25287081 None:intergenic 35.0%
CCACCATAGAATGCTATATT+TGG - chr5_4:25287077-25287096 None:intergenic 35.0%
CCAAATATAGCATTCTATGG+TGG + chr5_4:25287074-25287093 Msa0845870:CDS 35.0%
TTCATTAGACTGTGTTGAGA+TGG - chr5_4:25287129-25287148 None:intergenic 35.0%
AAGGAAACATTCAAGAGATG+GGG + chr5_4:25287165-25287184 Msa0845870:three_prime_UTR 35.0%
TTTCGTTCAAACAATGATGG+AGG + chr5_4:25287196-25287215 Msa0845870:three_prime_UTR 35.0%
CGTTCTCCCAAAAAGCTTTA+TGG - chr5_4:25285662-25285681 None:intergenic 40.0%
ATAAGGTATGTGCCATACCA+TGG + chr5_4:25285845-25285864 Msa0845870:intron 40.0%
TGAGTTTCACTGCGATTTCT+CGG + chr5_4:25285991-25286010 Msa0845870:intron 40.0%
TGAAAGAGATCTGTCCATCA+AGG + chr5_4:25286160-25286179 Msa0845870:intron 40.0%
CTCGATGTTTGTGTATGTGT+CGG + chr5_4:25286387-25286406 Msa0845870:intron 40.0%
AAAAGAACTCATCGAGGATG+AGG + chr5_4:25286512-25286531 Msa0845870:CDS 40.0%
ATTCTGAATCCTCTGTGGTT+TGG - chr5_4:25286545-25286564 None:intergenic 40.0%
! TTTCAGATGAGGGAGTTTTC+AGG - chr5_4:25286596-25286615 None:intergenic 40.0%
! ACAACTTGGTCTGGTTTCTT+TGG - chr5_4:25286684-25286703 None:intergenic 40.0%
ATCTTGGACATGGAACAACT+TGG - chr5_4:25286698-25286717 None:intergenic 40.0%
ATGTCCAAGATGCAAAAGCA+CGG + chr5_4:25286707-25286726 Msa0845870:CDS 40.0%
!!! CAATTTTTGCAGAAGTGTCG+AGG - chr5_4:25286774-25286793 None:intergenic 40.0%
GCCTATAAGCATATTTCTCG+TGG - chr5_4:25286823-25286842 None:intergenic 40.0%
AGAAATATGCTTATAGGCGC+AGG + chr5_4:25286825-25286844 Msa0845870:CDS 40.0%
! GTTTTCCTCAACAGTTTCCA+TGG + chr5_4:25287018-25287037 Msa0845870:CDS 40.0%
TAACACATAGCAGGATTCCA+TGG - chr5_4:25287038-25287057 None:intergenic 40.0%
AGCATTCTATGGTGGTTGTT+TGG + chr5_4:25287082-25287101 Msa0845870:CDS 40.0%
!! ACTGTGTTGAGATGGATTGT+AGG - chr5_4:25287121-25287140 None:intergenic 40.0%
! AATGATGGAGGACTTTCCAA+GGG + chr5_4:25287208-25287227 Msa0845870:three_prime_UTR 40.0%
CGTTCTCCCAAAAAGCTTTA+TGG - chr5_4:25285662-25285681 None:intergenic 40.0%
ATAAGGTATGTGCCATACCA+TGG + chr5_4:25285845-25285864 Msa0845870:intron 40.0%
TGAGTTTCACTGCGATTTCT+CGG + chr5_4:25285991-25286010 Msa0845870:intron 40.0%
TGAAAGAGATCTGTCCATCA+AGG + chr5_4:25286160-25286179 Msa0845870:intron 40.0%
CTCGATGTTTGTGTATGTGT+CGG + chr5_4:25286387-25286406 Msa0845870:intron 40.0%
AAAAGAACTCATCGAGGATG+AGG + chr5_4:25286512-25286531 Msa0845870:CDS 40.0%
ATTCTGAATCCTCTGTGGTT+TGG - chr5_4:25286545-25286564 None:intergenic 40.0%
! TTTCAGATGAGGGAGTTTTC+AGG - chr5_4:25286596-25286615 None:intergenic 40.0%
! ACAACTTGGTCTGGTTTCTT+TGG - chr5_4:25286684-25286703 None:intergenic 40.0%
ATCTTGGACATGGAACAACT+TGG - chr5_4:25286698-25286717 None:intergenic 40.0%
ATGTCCAAGATGCAAAAGCA+CGG + chr5_4:25286707-25286726 Msa0845870:CDS 40.0%
!!! CAATTTTTGCAGAAGTGTCG+AGG - chr5_4:25286774-25286793 None:intergenic 40.0%
GCCTATAAGCATATTTCTCG+TGG - chr5_4:25286823-25286842 None:intergenic 40.0%
AGAAATATGCTTATAGGCGC+AGG + chr5_4:25286825-25286844 Msa0845870:CDS 40.0%
! GTTTTCCTCAACAGTTTCCA+TGG + chr5_4:25287018-25287037 Msa0845870:CDS 40.0%
TAACACATAGCAGGATTCCA+TGG - chr5_4:25287038-25287057 None:intergenic 40.0%
AGCATTCTATGGTGGTTGTT+TGG + chr5_4:25287082-25287101 Msa0845870:CDS 40.0%
!! ACTGTGTTGAGATGGATTGT+AGG - chr5_4:25287121-25287140 None:intergenic 40.0%
! AATGATGGAGGACTTTCCAA+GGG + chr5_4:25287208-25287227 Msa0845870:three_prime_UTR 40.0%
GAGTTTCACTGCGATTTCTC+GGG + chr5_4:25285992-25286011 Msa0845870:intron 45.0%
AGTTTCACTGCGATTTCTCG+GGG + chr5_4:25285993-25286012 Msa0845870:intron 45.0%
GACGTCTCTGTTTCAGATGA+GGG - chr5_4:25286606-25286625 None:intergenic 45.0%
AGACGTCTCTGTTTCAGATG+AGG - chr5_4:25286607-25286626 None:intergenic 45.0%
! GTGCTTTTGCATCTTGGACA+TGG - chr5_4:25286708-25286727 None:intergenic 45.0%
TGCCAAAGATACTGGACTTC+TGG + chr5_4:25286792-25286811 Msa0845870:CDS 45.0%
CTCCAGAAGTCCAGTATCTT+TGG - chr5_4:25286797-25286816 None:intergenic 45.0%
CAAAGATACTGGACTTCTGG+AGG + chr5_4:25286795-25286814 Msa0845870:CDS 45.0%
GGATTCCATGGAAACTGTTG+AGG - chr5_4:25287026-25287045 None:intergenic 45.0%
GTGGTTGTTTGGTACCATCT+TGG + chr5_4:25287093-25287112 Msa0845870:CDS 45.0%
! ATGGATTGTAGGCTCCAAGA+TGG - chr5_4:25287110-25287129 None:intergenic 45.0%
! CAATGATGGAGGACTTTCCA+AGG + chr5_4:25287207-25287226 Msa0845870:three_prime_UTR 45.0%
AGCGTTAGAAGCAAAACCCT+TGG - chr5_4:25287227-25287246 None:intergenic 45.0%
! CTAACGCTGCAGCTTTATCA+AGG + chr5_4:25287239-25287258 Msa0845870:three_prime_UTR 45.0%
GAGTTTCACTGCGATTTCTC+GGG + chr5_4:25285992-25286011 Msa0845870:intron 45.0%
AGTTTCACTGCGATTTCTCG+GGG + chr5_4:25285993-25286012 Msa0845870:intron 45.0%
GACGTCTCTGTTTCAGATGA+GGG - chr5_4:25286606-25286625 None:intergenic 45.0%
AGACGTCTCTGTTTCAGATG+AGG - chr5_4:25286607-25286626 None:intergenic 45.0%
! GTGCTTTTGCATCTTGGACA+TGG - chr5_4:25286708-25286727 None:intergenic 45.0%
TGCCAAAGATACTGGACTTC+TGG + chr5_4:25286792-25286811 Msa0845870:CDS 45.0%
CTCCAGAAGTCCAGTATCTT+TGG - chr5_4:25286797-25286816 None:intergenic 45.0%
CAAAGATACTGGACTTCTGG+AGG + chr5_4:25286795-25286814 Msa0845870:CDS 45.0%
GGATTCCATGGAAACTGTTG+AGG - chr5_4:25287026-25287045 None:intergenic 45.0%
GTGGTTGTTTGGTACCATCT+TGG + chr5_4:25287093-25287112 Msa0845870:CDS 45.0%
! ATGGATTGTAGGCTCCAAGA+TGG - chr5_4:25287110-25287129 None:intergenic 45.0%
! CAATGATGGAGGACTTTCCA+AGG + chr5_4:25287207-25287226 Msa0845870:three_prime_UTR 45.0%
AGCGTTAGAAGCAAAACCCT+TGG - chr5_4:25287227-25287246 None:intergenic 45.0%
! CTAACGCTGCAGCTTTATCA+AGG + chr5_4:25287239-25287258 Msa0845870:three_prime_UTR 45.0%
GGCTTCATTCCAAACCACAG+AGG + chr5_4:25286533-25286552 Msa0845870:CDS 50.0%
CGGAGATTCTGAATCCTCTG+TGG - chr5_4:25286550-25286569 None:intergenic 50.0%
GGACATGGAACAACTTGGTC+TGG - chr5_4:25286693-25286712 None:intergenic 50.0%
! GTGTCCGTGCTTTTGCATCT+TGG - chr5_4:25286714-25286733 None:intergenic 50.0%
GGCACAGGATAACACATAGC+AGG - chr5_4:25287047-25287066 None:intergenic 50.0%
GGCTTCATTCCAAACCACAG+AGG + chr5_4:25286533-25286552 Msa0845870:CDS 50.0%
CGGAGATTCTGAATCCTCTG+TGG - chr5_4:25286550-25286569 None:intergenic 50.0%
GGACATGGAACAACTTGGTC+TGG - chr5_4:25286693-25286712 None:intergenic 50.0%
! GTGTCCGTGCTTTTGCATCT+TGG - chr5_4:25286714-25286733 None:intergenic 50.0%
GGCACAGGATAACACATAGC+AGG - chr5_4:25287047-25287066 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 25285643 25287312 25285643 ID=Msa0845870;Name=Msa0845870
chr5_4 mRNA 25285643 25287312 25285643 ID=Msa0845870-mRNA-1;Parent=Msa0845870;Name=Msa0845870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.5|1|1|1|2|152|288
chr5_4 exon 25285643 25285849 25285643 ID=Msa0845870-mRNA-1:exon:10049;Parent=Msa0845870-mRNA-1
chr5_4 exon 25286501 25287312 25286501 ID=Msa0845870-mRNA-1:exon:10050;Parent=Msa0845870-mRNA-1
chr5_4 CDS 25285643 25285849 25285643 ID=Msa0845870-mRNA-1:cds;Parent=Msa0845870-mRNA-1
chr5_4 CDS 25286501 25287160 25286501 ID=Msa0845870-mRNA-1:cds;Parent=Msa0845870-mRNA-1
chr5_4 three_prime_UTR 25287161 25287312 25287161 ID=Msa0845870-mRNA-1:three_prime_utr;Parent=Msa0845870-mRNA-1
Gene Sequence

>Msa0845870

ATGATAAACAATTCAACCATAAAGCTTTTTGGGAGAACGATTTTTCAAACACACAACACTGATGTGTCTATCAATGATTCTTCCTCAGAATTTACTCCTACTATTGCTCATGAAGATTTCTCTGATCATAGTAGTCATTCATCTTTATCTGCATCTTCTCCACTTGAAGTGAATTCTTCAACTGAACATGATGCAAAGAGATATAAGGAAACATCAAGAAAAGAACTCATCGAGGATGAGGCTTCATTCCAAACCACAGAGGATTCAGAATCTCCGACATCTTCAAGCCTTATTGAGAACCTGAAAACTCCCTCATCTGAAACAGAGACGTCTCAATCAATTTCCACTAAAATTGATAAACAAACTGATATGTCACAAGAAAAATCTCCAAAGAAACCAGACCAAGTTGTTCCATGTCCAAGATGCAAAAGCACGGACACAAAATTTTGCTACTACAACAACTACAACGTCAAACAGCCTCGACACTTCTGCAAAAATTGCCAAAGATACTGGACTTCTGGAGGAACCACGAGAAATATGCTTATAGGCGCAGGTCGCCGCAAGAACAGAATCTCTTCTTCTTTTCATTCTGATACCTCACATCATCATCAAATGAGTACTGTCTTGACATTTGGATCGGATTCTCCTATTATGTCTTCAACTTCACTTCACAAAAAAGGGAATGTTGGTTCACTTGAAAAAACATTTGACAAGAGTTATCAAAGTTTTCCTCAACAGTTTCCATGGAATCCTGCTATGTGTTATCCTGTGCCAATCTATCCAAATATAGCATTCTATGGTGGTTGTTTGGTACCATCTTGGAGCCTACAATCCATCTCAACACAGTCTAATGAACCAAATAACTAA
Protein sequence

>Msa0845870

MINNSTIKLFGRTIFQTHNTDVSINDSSSEFTPTIAHEDFSDHSSHSSLSASSPLEVNSSTEHDAKRYKETSRKELIEDEASFQTTEDSESPTSSSLIENLKTPSSETETSQSISTKIDKQTDMSQEKSPKKPDQVVPCPRCKSTDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGTTRNMLIGAGRRKNRISSSFHSDTSHHHQMSTVLTFGSDSPIMSSTSLHKKGNVGSLEKTFDKSYQSFPQQFPWNPAMCYPVPIYPNIAFYGGCLVPSWSLQSISTQSNEPNN*