Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0858830 | Msa0858840 | 0.817829 | 2.691202e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCCTCATTTCATAATCT+TGG | 0.259074 | 5_4:+56685513 | None:intergenic |
| TTCAAATACTCCTCTGGCTT+TGG | 0.276682 | 5_4:-56685032 | Msa0858830:CDS |
| CATTCTTGGAAGAAGCAAAA+TGG | 0.310539 | 5_4:+56684991 | None:intergenic |
| CCTTTCTTGCAAGAAATACT+TGG | 0.363433 | 5_4:+56685447 | None:intergenic |
| AAGACTCATAAATGCATTCT+TGG | 0.368144 | 5_4:+56684977 | None:intergenic |
| AAATACTTGGTCCGACATAT+TGG | 0.368493 | 5_4:+56685460 | None:intergenic |
| AAGCAAAATGGTTTGCTTCT+CGG | 0.368539 | 5_4:+56685003 | None:intergenic |
| CCAAGTATTTCTTGCAAGAA+AGG | 0.377212 | 5_4:-56685447 | Msa0858830:CDS |
| CCTCATTTCATAATCTTGGT+TGG | 0.436906 | 5_4:+56685517 | None:intergenic |
| GTATTCTTCAAATACTCCTC+TGG | 0.446060 | 5_4:-56685038 | Msa0858830:CDS |
| TCTTCCTCATGAGTCATTGA+AGG | 0.474531 | 5_4:+56685392 | None:intergenic |
| TTCAATGACTCATGAGGAAG+AGG | 0.520203 | 5_4:-56685390 | Msa0858830:intron |
| ATTTCTTGCAAGAAAGGCAA+AGG | 0.564591 | 5_4:-56685441 | Msa0858830:CDS |
| CCAACCAAGATTATGAAATG+AGG | 0.575459 | 5_4:-56685517 | Msa0858830:exon |
| TGATCCTTCAATGACTCATG+AGG | 0.582376 | 5_4:-56685396 | Msa0858830:CDS |
| ATATACTCTAACCAATATGT+CGG | 0.607580 | 5_4:-56685471 | Msa0858830:CDS |
| CACAAGCATCACGTCTACCT+CGG | 0.607932 | 5_4:-56685062 | Msa0858830:CDS |
| TTGCAAGAAAGGCAAAGGTG+TGG | 0.616875 | 5_4:-56685436 | Msa0858830:CDS |
| TCGGAATGTTCCAAAGCCAG+AGG | 0.668181 | 5_4:+56685022 | None:intergenic |
| AGTATTTGAAGAATACACCG+AGG | 0.801873 | 5_4:+56685045 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGACAAATATTTTTTTAATT+TGG | - | chr5_4:56685382-56685401 | Msa0858830:intron | 10.0% |
| !! | AAAAAATTTCCAAAAAATGA+AGG | + | chr5_4:56685237-56685256 | None:intergenic | 15.0% |
| !!! | TTAGAAATTTTTTAAGTTGA+GGG | - | chr5_4:56685293-56685312 | Msa0858830:intron | 15.0% |
| !! | TTAAAAAGAAGAAAAAGAAG+TGG | + | chr5_4:56684994-56685013 | None:intergenic | 20.0% |
| !! | CTCAACTTAAAAAATTTCTA+AGG | + | chr5_4:56685294-56685313 | None:intergenic | 20.0% |
| !!! | CTTAGAAATTTTTTAAGTTG+AGG | - | chr5_4:56685292-56685311 | Msa0858830:intron | 20.0% |
| ! | ATATACTCTAACCAATATGT+CGG | - | chr5_4:56685073-56685092 | Msa0858830:intron | 25.0% |
| ! | AATAATGAATACCTGAAACA+AGG | + | chr5_4:56685443-56685462 | None:intergenic | 25.0% |
| ! | TCATTATTAAATTCGTCTGA+AGG | - | chr5_4:56685455-56685474 | Msa0858830:CDS | 25.0% |
| TCTTCCTCATTTCATAATCT+TGG | + | chr5_4:56685034-56685053 | None:intergenic | 30.0% | |
| TCTATTCTCATTATGTTGCT+TGG | - | chr5_4:56685188-56685207 | Msa0858830:intron | 30.0% | |
| !!! | ATTAATGCGCCTTCATTTTT+TGG | - | chr5_4:56685225-56685244 | Msa0858830:intron | 30.0% |
| !!! | ATTAGTCTTTTGTCTTCCAA+GGG | - | chr5_4:56685339-56685358 | Msa0858830:intron | 30.0% |
| CCTCATTTCATAATCTTGGT+TGG | + | chr5_4:56685030-56685049 | None:intergenic | 35.0% | |
| CCAACCAAGATTATGAAATG+AGG | - | chr5_4:56685027-56685046 | Msa0858830:CDS | 35.0% | |
| AAATACTTGGTCCGACATAT+TGG | + | chr5_4:56685087-56685106 | None:intergenic | 35.0% | |
| CCTTTCTTGCAAGAAATACT+TGG | + | chr5_4:56685100-56685119 | None:intergenic | 35.0% | |
| CCAAGTATTTCTTGCAAGAA+AGG | - | chr5_4:56685097-56685116 | Msa0858830:intron | 35.0% | |
| ATTTCTTGCAAGAAAGGCAA+AGG | - | chr5_4:56685103-56685122 | Msa0858830:intron | 35.0% | |
| !!! | TAAGTTGAGGGTTTTGTTGA+TGG | - | chr5_4:56685305-56685324 | Msa0858830:intron | 35.0% |
| !!! | CATTAGTCTTTTGTCTTCCA+AGG | - | chr5_4:56685338-56685357 | Msa0858830:intron | 35.0% |
| ATAAGATGACGAACTAATGG+AGG | + | chr5_4:56685412-56685431 | None:intergenic | 35.0% | |
| TGGATAAGATGACGAACTAA+TGG | + | chr5_4:56685415-56685434 | None:intergenic | 35.0% | |
| !!! | TATCCATTTTGCCTTGTTTC+AGG | - | chr5_4:56685429-56685448 | Msa0858830:CDS | 35.0% |
| ATACCTGAAACAAGGCAAAA+TGG | + | chr5_4:56685435-56685454 | None:intergenic | 35.0% | |
| AGTATTTGAAGAATACACCG+AGG | + | chr5_4:56685502-56685521 | None:intergenic | 35.0% | |
| GTATTCTTCAAATACTCCTC+TGG | - | chr5_4:56685506-56685525 | Msa0858830:exon | 35.0% | |
| !! | AAGCAAAATGGTTTGCTTCT+CGG | + | chr5_4:56685544-56685563 | None:intergenic | 35.0% |
| CATTCTTGGAAGAAGCAAAA+TGG | + | chr5_4:56685556-56685575 | None:intergenic | 35.0% | |
| TGATCCTTCAATGACTCATG+AGG | - | chr5_4:56685148-56685167 | Msa0858830:intron | 40.0% | |
| TCTTCCTCATGAGTCATTGA+AGG | + | chr5_4:56685155-56685174 | None:intergenic | 40.0% | |
| TTCAATGACTCATGAGGAAG+AGG | - | chr5_4:56685154-56685173 | Msa0858830:intron | 40.0% | |
| ! | TTATGTTGCTTGGTTGCATG+TGG | - | chr5_4:56685198-56685217 | Msa0858830:intron | 40.0% |
| !! | TTTGTCTGAGCGTTGAACAA+GGG | + | chr5_4:56685369-56685388 | None:intergenic | 40.0% |
| !! | ATTTGTCTGAGCGTTGAACA+AGG | + | chr5_4:56685370-56685389 | None:intergenic | 40.0% |
| ! | TTCAAATACTCCTCTGGCTT+TGG | - | chr5_4:56685512-56685531 | Msa0858830:exon | 40.0% |
| TTGCAAGAAAGGCAAAGGTG+TGG | - | chr5_4:56685108-56685127 | Msa0858830:intron | 45.0% | |
| !! | TTGTCTGAGCGTTGAACAAG+GGG | + | chr5_4:56685368-56685387 | None:intergenic | 45.0% |
| GTTGAACAAGGGGAATCCCT+TGG | + | chr5_4:56685358-56685377 | None:intergenic | 50.0% | |
| CACAAGCATCACGTCTACCT+CGG | - | chr5_4:56685482-56685501 | Msa0858830:CDS | 50.0% | |
| TCGGAATGTTCCAAAGCCAG+AGG | + | chr5_4:56685525-56685544 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 56684982 | 56685584 | 56684982 | ID=Msa0858830;Name=Msa0858830 |
| chr5_4 | mRNA | 56684982 | 56685584 | 56684982 | ID=Msa0858830-mRNA-1;Parent=Msa0858830;Name=Msa0858830-mRNA-1;_AED=0.38;_eAED=0.60;_QI=62|0|0.5|1|1|1|2|0|79 |
| chr5_4 | exon | 56684982 | 56685089 | 56684982 | ID=Msa0858830-mRNA-1:exon:17371;Parent=Msa0858830-mRNA-1 |
| chr5_4 | exon | 56685391 | 56685584 | 56685391 | ID=Msa0858830-mRNA-1:exon:17370;Parent=Msa0858830-mRNA-1 |
| chr5_4 | five_prime_UTR | 56685523 | 56685584 | 56685523 | ID=Msa0858830-mRNA-1:five_prime_utr;Parent=Msa0858830-mRNA-1 |
| chr5_4 | CDS | 56685391 | 56685522 | 56685391 | ID=Msa0858830-mRNA-1:cds;Parent=Msa0858830-mRNA-1 |
| chr5_4 | CDS | 56684982 | 56685089 | 56684982 | ID=Msa0858830-mRNA-1:cds;Parent=Msa0858830-mRNA-1 |
| Gene Sequence |
| Protein sequence |
