AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0858840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCCTCATTTCATAATCT+TGG 0.259074 5_4:+56695865 None:intergenic
CATTCTTTGAAGAAGCAAAA+TGG 0.312978 5_4:+56695321 None:intergenic
TTCAAATGCTCCTCTGCCTT+TGG 0.317129 5_4:-56695362 Msa0858840:CDS
AAAACTAACAATTTGTTAGT+TGG 0.351135 5_4:+56695996 None:intergenic
CCTTTCTTGCAAGAAATACT+TGG 0.363433 5_4:+56695799 None:intergenic
TGTTGTGTCCTTATCAAGAT+TGG 0.368222 5_4:-56696103 None:intergenic
AAATACTTGGTCCGACATAT+TGG 0.368493 5_4:+56695812 None:intergenic
AAGCAAAATGGTTTGCTTCT+CGG 0.368539 5_4:+56695333 None:intergenic
CCAAGTATTTCTTGCAAGAA+AGG 0.377212 5_4:-56695799 Msa0858840:CDS
CCTCATTTCATAATCTTGGT+TGG 0.436906 5_4:+56695869 None:intergenic
TCTTCCTCATGAGTCATTGA+AGG 0.474531 5_4:+56695744 None:intergenic
TGGTGGTGGAGAAGAGATAT+AGG 0.485148 5_4:+56696049 None:intergenic
ATGTGCAAGAATATATATAG+AGG 0.485343 5_4:+56695946 None:intergenic
TAGTGGATCCAATCTTGATA+AGG 0.509800 5_4:+56696095 None:intergenic
TTGATTGGGTGCGATGGTGG+TGG 0.514450 5_4:+56696035 None:intergenic
TTCAATGACTCATGAGGAAG+AGG 0.520203 5_4:-56695742 Msa0858840:intron
CACAATGATTTCATCAGTAG+TGG 0.524252 5_4:+56696078 None:intergenic
ATTTCTTGCAAGAAAGGCAA+AGG 0.564591 5_4:-56695793 Msa0858840:CDS
CCAACCAAGATTATGAAATG+AGG 0.575459 5_4:-56695869 Msa0858840:exon
TGATCCTTCAATGACTCATG+AGG 0.582376 5_4:-56695748 Msa0858840:CDS
TGCTTCTCGGAATGTTCCAA+AGG 0.583114 5_4:+56695346 None:intergenic
TCGGAATGTTCCAAAGGCAG+AGG 0.588258 5_4:+56695352 None:intergenic
ATATACTCTAACCAATATGT+CGG 0.607580 5_4:-56695823 Msa0858840:CDS
TTGCAAGAAAGGCAAAGGTG+TGG 0.616875 5_4:-56695788 Msa0858840:CDS
ATGCACGACAGTGAGCACTG+AGG 0.825154 5_4:+56695387 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGATTTGAATTTTGATT+GGG + chr5_4:56695395-56695414 None:intergenic 15.0%
!! ATTAAAAAAAGAAAAAGAAG+TGG + chr5_4:56695512-56695531 None:intergenic 15.0%
!!! TTTGAAAATTTTTAAGTTGA+GGG - chr5_4:56695821-56695840 Msa0858840:CDS 15.0%
!!! ATTTGATTTGAATTTTGATT+GGG + chr5_4:56695395-56695414 None:intergenic 15.0%
!! ATTAAAAAAAGAAAAAGAAG+TGG + chr5_4:56695512-56695531 None:intergenic 15.0%
!!! TTTGAAAATTTTTAAGTTGA+GGG - chr5_4:56695821-56695840 Msa0858840:CDS 15.0%
!!! GATTTGATTTGAATTTTGAT+TGG + chr5_4:56695396-56695415 None:intergenic 20.0%
!!! AAAACTAACAATTTGTTAGT+TGG + chr5_4:56695420-56695439 None:intergenic 20.0%
!!! CTTTGAAAATTTTTAAGTTG+AGG - chr5_4:56695820-56695839 Msa0858840:CDS 20.0%
!! AATAATAAATACCTGAAACA+AGG + chr5_4:56695970-56695989 None:intergenic 20.0%
!! TTATTATTAAATTCGTCTGA+AGG - chr5_4:56695982-56696001 Msa0858840:five_prime_UTR 20.0%
!!! GATTTGATTTGAATTTTGAT+TGG + chr5_4:56695396-56695415 None:intergenic 20.0%
!!! AAAACTAACAATTTGTTAGT+TGG + chr5_4:56695420-56695439 None:intergenic 20.0%
!!! CTTTGAAAATTTTTAAGTTG+AGG - chr5_4:56695820-56695839 Msa0858840:CDS 20.0%
!! AATAATAAATACCTGAAACA+AGG + chr5_4:56695970-56695989 None:intergenic 20.0%
!! TTATTATTAAATTCGTCTGA+AGG - chr5_4:56695982-56696001 Msa0858840:five_prime_UTR 20.0%
! ATGTGCAAGAATATATATAG+AGG + chr5_4:56695470-56695489 None:intergenic 25.0%
! ATATACTCTAACCAATATGT+CGG - chr5_4:56695590-56695609 Msa0858840:intron 25.0%
! ATGTGCAAGAATATATATAG+AGG + chr5_4:56695470-56695489 None:intergenic 25.0%
! ATATACTCTAACCAATATGT+CGG - chr5_4:56695590-56695609 Msa0858840:intron 25.0%
TCTTCCTCATTTCATAATCT+TGG + chr5_4:56695551-56695570 None:intergenic 30.0%
TCTATTCTCATTATGTTGCT+TGG - chr5_4:56695705-56695724 Msa0858840:intron 30.0%
!!! ATTAGTCTTTTGTCTTCCAA+GGG - chr5_4:56695867-56695886 Msa0858840:exon 30.0%
TTGATAAGATGACGAACTAA+TGG + chr5_4:56695942-56695961 None:intergenic 30.0%
!!! TATCAATTTTGCCTTGTTTC+AGG - chr5_4:56695956-56695975 Msa0858840:five_prime_UTR 30.0%
! CATTCTTTGAAGAAGCAAAA+TGG + chr5_4:56696095-56696114 None:intergenic 30.0%
TCTTCCTCATTTCATAATCT+TGG + chr5_4:56695551-56695570 None:intergenic 30.0%
TCTATTCTCATTATGTTGCT+TGG - chr5_4:56695705-56695724 Msa0858840:intron 30.0%
!!! ATTAGTCTTTTGTCTTCCAA+GGG - chr5_4:56695867-56695886 Msa0858840:exon 30.0%
TTGATAAGATGACGAACTAA+TGG + chr5_4:56695942-56695961 None:intergenic 30.0%
!!! TATCAATTTTGCCTTGTTTC+AGG - chr5_4:56695956-56695975 Msa0858840:five_prime_UTR 30.0%
! CATTCTTTGAAGAAGCAAAA+TGG + chr5_4:56696095-56696114 None:intergenic 30.0%
! TAGTGGATCCAATCTTGATA+AGG + chr5_4:56695321-56695340 None:intergenic 35.0%
CACAATGATTTCATCAGTAG+TGG + chr5_4:56695338-56695357 None:intergenic 35.0%
CCTCATTTCATAATCTTGGT+TGG + chr5_4:56695547-56695566 None:intergenic 35.0%
CCAACCAAGATTATGAAATG+AGG - chr5_4:56695544-56695563 Msa0858840:intron 35.0%
AAATACTTGGTCCGACATAT+TGG + chr5_4:56695604-56695623 None:intergenic 35.0%
CCTTTCTTGCAAGAAATACT+TGG + chr5_4:56695617-56695636 None:intergenic 35.0%
CCAAGTATTTCTTGCAAGAA+AGG - chr5_4:56695614-56695633 Msa0858840:intron 35.0%
ATTTCTTGCAAGAAAGGCAA+AGG - chr5_4:56695620-56695639 Msa0858840:intron 35.0%
!!! TAAGTTGAGGGTTTTGTTGA+TGG - chr5_4:56695833-56695852 Msa0858840:CDS 35.0%
!!! CATTAGTCTTTTGTCTTCCA+AGG - chr5_4:56695866-56695885 Msa0858840:exon 35.0%
ATAAGATGACGAACTAATGG+AGG + chr5_4:56695939-56695958 None:intergenic 35.0%
!! AAGCAAAATGGTTTGCTTCT+CGG + chr5_4:56696083-56696102 None:intergenic 35.0%
! TAGTGGATCCAATCTTGATA+AGG + chr5_4:56695321-56695340 None:intergenic 35.0%
CACAATGATTTCATCAGTAG+TGG + chr5_4:56695338-56695357 None:intergenic 35.0%
CCTCATTTCATAATCTTGGT+TGG + chr5_4:56695547-56695566 None:intergenic 35.0%
CCAACCAAGATTATGAAATG+AGG - chr5_4:56695544-56695563 Msa0858840:intron 35.0%
AAATACTTGGTCCGACATAT+TGG + chr5_4:56695604-56695623 None:intergenic 35.0%
CCTTTCTTGCAAGAAATACT+TGG + chr5_4:56695617-56695636 None:intergenic 35.0%
CCAAGTATTTCTTGCAAGAA+AGG - chr5_4:56695614-56695633 Msa0858840:intron 35.0%
ATTTCTTGCAAGAAAGGCAA+AGG - chr5_4:56695620-56695639 Msa0858840:intron 35.0%
!!! TAAGTTGAGGGTTTTGTTGA+TGG - chr5_4:56695833-56695852 Msa0858840:CDS 35.0%
!!! CATTAGTCTTTTGTCTTCCA+AGG - chr5_4:56695866-56695885 Msa0858840:exon 35.0%
ATAAGATGACGAACTAATGG+AGG + chr5_4:56695939-56695958 None:intergenic 35.0%
!! AAGCAAAATGGTTTGCTTCT+CGG + chr5_4:56696083-56696102 None:intergenic 35.0%
!!! TGAATTTTGATTGGGTGCGA+TGG + chr5_4:56695387-56695406 None:intergenic 40.0%
TGATCCTTCAATGACTCATG+AGG - chr5_4:56695665-56695684 Msa0858840:intron 40.0%
TCTTCCTCATGAGTCATTGA+AGG + chr5_4:56695672-56695691 None:intergenic 40.0%
TTCAATGACTCATGAGGAAG+AGG - chr5_4:56695671-56695690 Msa0858840:intron 40.0%
! TTATGTTGCTTGGTTGCATG+TGG - chr5_4:56695715-56695734 Msa0858840:intron 40.0%
!!! TGAATTTTGATTGGGTGCGA+TGG + chr5_4:56695387-56695406 None:intergenic 40.0%
TGATCCTTCAATGACTCATG+AGG - chr5_4:56695665-56695684 Msa0858840:intron 40.0%
TCTTCCTCATGAGTCATTGA+AGG + chr5_4:56695672-56695691 None:intergenic 40.0%
TTCAATGACTCATGAGGAAG+AGG - chr5_4:56695671-56695690 Msa0858840:intron 40.0%
! TTATGTTGCTTGGTTGCATG+TGG - chr5_4:56695715-56695734 Msa0858840:intron 40.0%
!! TGGTGGTGGAGAAGAGATAT+AGG + chr5_4:56695367-56695386 None:intergenic 45.0%
!!! ATTTTGATTGGGTGCGATGG+TGG + chr5_4:56695384-56695403 None:intergenic 45.0%
TTGCAAGAAAGGCAAAGGTG+TGG - chr5_4:56695625-56695644 Msa0858840:intron 45.0%
TCTGTCTGAGCCTTGAACAA+GGG + chr5_4:56695897-56695916 None:intergenic 45.0%
ATCTGTCTGAGCCTTGAACA+AGG + chr5_4:56695898-56695917 None:intergenic 45.0%
TTCAAATGCTCCTCTGCCTT+TGG - chr5_4:56696051-56696070 Msa0858840:five_prime_UTR 45.0%
TGCTTCTCGGAATGTTCCAA+AGG + chr5_4:56696070-56696089 None:intergenic 45.0%
!! TGGTGGTGGAGAAGAGATAT+AGG + chr5_4:56695367-56695386 None:intergenic 45.0%
!!! ATTTTGATTGGGTGCGATGG+TGG + chr5_4:56695384-56695403 None:intergenic 45.0%
TTGCAAGAAAGGCAAAGGTG+TGG - chr5_4:56695625-56695644 Msa0858840:intron 45.0%
TCTGTCTGAGCCTTGAACAA+GGG + chr5_4:56695897-56695916 None:intergenic 45.0%
ATCTGTCTGAGCCTTGAACA+AGG + chr5_4:56695898-56695917 None:intergenic 45.0%
TTCAAATGCTCCTCTGCCTT+TGG - chr5_4:56696051-56696070 Msa0858840:five_prime_UTR 45.0%
TGCTTCTCGGAATGTTCCAA+AGG + chr5_4:56696070-56696089 None:intergenic 45.0%
!! AAAAAAATAAAATAAATGAA+AGG + chr5_4:56695754-56695773 None:intergenic 5.0%
!!! TTTCATTTATTTTATTTTTT+TGG - chr5_4:56695753-56695772 Msa0858840:CDS 5.0%
!! AAAAAAATAAAATAAATGAA+AGG + chr5_4:56695754-56695773 None:intergenic 5.0%
!!! TTTCATTTATTTTATTTTTT+TGG - chr5_4:56695753-56695772 Msa0858840:CDS 5.0%
CTTGAACAAGGGGAATCCCT+TGG + chr5_4:56695886-56695905 None:intergenic 50.0%
CAAGGGATTCCCCTTGTTCA+AGG - chr5_4:56695884-56695903 Msa0858840:five_prime_UTR 50.0%
CTGTCTGAGCCTTGAACAAG+GGG + chr5_4:56695896-56695915 None:intergenic 50.0%
TCGGAATGTTCCAAAGGCAG+AGG + chr5_4:56696064-56696083 None:intergenic 50.0%
CTTGAACAAGGGGAATCCCT+TGG + chr5_4:56695886-56695905 None:intergenic 50.0%
CAAGGGATTCCCCTTGTTCA+AGG - chr5_4:56695884-56695903 Msa0858840:five_prime_UTR 50.0%
CTGTCTGAGCCTTGAACAAG+GGG + chr5_4:56695896-56695915 None:intergenic 50.0%
TCGGAATGTTCCAAAGGCAG+AGG + chr5_4:56696064-56696083 None:intergenic 50.0%
! TTGATTGGGTGCGATGGTGG+TGG + chr5_4:56695381-56695400 None:intergenic 55.0%
! ATGCACGACAGTGAGCACTG+AGG + chr5_4:56696029-56696048 None:intergenic 55.0%
! TTGATTGGGTGCGATGGTGG+TGG + chr5_4:56695381-56695400 None:intergenic 55.0%
! ATGCACGACAGTGAGCACTG+AGG + chr5_4:56696029-56696048 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 56695315 56696120 56695315 ID=Msa0858840;Name=Msa0858840
chr5_4 mRNA 56695315 56696120 56695315 ID=Msa0858840-mRNA-1;Parent=Msa0858840;Name=Msa0858840-mRNA-1;_AED=0.31;_eAED=0.47;_QI=246|0|0.5|1|1|1|2|0|82
chr5_4 exon 56695315 56695431 56695315 ID=Msa0858840-mRNA-1:exon:17369;Parent=Msa0858840-mRNA-1
chr5_4 exon 56695743 56696120 56695743 ID=Msa0858840-mRNA-1:exon:17368;Parent=Msa0858840-mRNA-1
chr5_4 five_prime_UTR 56695875 56696120 56695875 ID=Msa0858840-mRNA-1:five_prime_utr;Parent=Msa0858840-mRNA-1
chr5_4 CDS 56695743 56695874 56695743 ID=Msa0858840-mRNA-1:cds;Parent=Msa0858840-mRNA-1
chr5_4 CDS 56695315 56695431 56695315 ID=Msa0858840-mRNA-1:cds;Parent=Msa0858840-mRNA-1
Gene Sequence

>Msa0858840

ATGAGGAAGAAGAGAGAGCAAATTATAATATATACTCTAACCAATATGTCGGACCAAGTATTTCTTGCAAGAAAGGCAAAGGTGTGGAAAACAGTTCTCAAAAATGATCCTTCAATGACTCATGAGGAAGAGGCGTGCACAAGCATCACGTCTACCTCAGTGCTCACTGTCGTGCATTCTTCAAATGCTCCTCTGCCTTTGGAACATTCCGAGAAGCAAACCATTTTGCTTCTTCAAAGAATGCGTTGA

Protein sequence

>Msa0858840

MRKKREQIIIYTLTNMSDQVFLARKAKVWKTVLKNDPSMTHEEEACTSITSTSVLTVVHSSNAPLPLEHSEKQTILLLQRMR*