AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0860800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0860800 MtrunA17_Chr5g0440321 75.637 353 10 4 1 353 1 277 1.66e-165 462
Msa0860800 MtrunA17_Chr5g0440311 35.057 348 137 13 1 342 1 265 1.36e-35 130
Msa0860800 MtrunA17_Chr5g0440291 44.545 110 58 2 1 108 3 111 4.91e-25 102
Msa0860800 MtrunA17_Chr6g0481571 32.558 129 79 3 1 126 1 124 1.96e-14 70.5
Msa0860800 MtrunA17_Chr8g0356011 28.571 231 125 8 4 225 15 214 1.31e-12 68.6
Msa0860800 MtrunA17_Chr7g0253661 37.209 86 52 1 10 93 8 93 2.42e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0860800 AT2G27140.1 42.361 144 67 4 9 144 10 145 2.90e-26 104
Msa0860800 AT5G20970.1 39.844 128 73 3 10 135 7 132 5.40e-19 85.5

Find 91 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTCTCTTCTATAGCTTT+TGG 0.148334 5_4:-59877372 Msa0860810:three_prime_UTR
ACTTTCTCTTCTTTAGCTTT+TGG 0.157965 5_4:-59877138 Msa0860810:three_prime_UTR
GCTTTCTCTTCTTTAGCTTT+TGG 0.158375 5_4:-59877249 Msa0860810:three_prime_UTR
AGCAATAAACTTGGCATCTT+TGG 0.197304 5_4:-59877565 Msa0860810:three_prime_UTR
TTTGGTCTTGGGGCTTCTTC+TGG 0.224293 5_4:-59877078 Msa0860810:three_prime_UTR
ATCTTATTGGTCGTGGGACT+TGG 0.235571 5_4:+59877717 Msa0860800:CDS
TTTGGTCTTGGAGCTTCTTC+TGG 0.244570 5_4:-59877189 Msa0860810:three_prime_UTR
TTTGGTCTTGGAGCTTCTTC+TGG 0.244570 5_4:-59877300 Msa0860810:three_prime_UTR
TGCGATGGTTCCTTTGGTCT+TGG 0.253322 5_4:-59877090 Msa0860810:three_prime_UTR
TATTTATCCCATTCATGATA+TGG 0.253330 5_4:-59876580 None:intergenic
ACAAAGGCATAAATAATAAA+TGG 0.280026 5_4:+59876510 Msa0860800:exon
GCGATGGTTCCTTTGGTCTT+GGG 0.287083 5_4:-59877089 Msa0860810:three_prime_UTR
CTTATCATGCAGGATATAGA+AGG 0.298323 5_4:+59876845 Msa0860800:intron
TGCGATGGTTCCTTTGGTCT+TGG 0.307717 5_4:-59877201 Msa0860810:three_prime_UTR
CTTTATTGTAGAAATACATT+TGG 0.339189 5_4:+59877741 Msa0860800:CDS
GTCTTTCACCTATCAGCCTT+AGG 0.347092 5_4:-59876903 None:intergenic
TGTGGCGATTCCTTTGGTCT+TGG 0.352071 5_4:-59877312 Msa0860810:three_prime_UTR
GGATTTGTTGTTGTTTCCTT+AGG 0.356840 5_4:-59877054 None:intergenic
ATGTGTCATGCTAATCTTAT+TGG 0.363566 5_4:+59877704 Msa0860800:CDS
TCATAGACACGATCAGCTTC+TGG 0.364950 5_4:-59876547 None:intergenic
TGTAGATTTCACTCTCTCTA+AGG 0.366735 5_4:+59877605 Msa0860800:CDS
CTCTAAGGCTCTCTGTAATC+AGG 0.374231 5_4:+59877620 Msa0860800:CDS
TGGATCGTCTCAGGGAGAAT+TGG 0.395265 5_4:+59877761 Msa0860800:CDS
AAATACATTTGGATCGTCTC+AGG 0.407520 5_4:+59877752 Msa0860800:CDS
ACAAAAGGCTGATGAGCAAA+AGG 0.421804 5_4:+59877332 Msa0860800:CDS
AAACCTGCCCTAAGGCTGAT+AGG 0.427961 5_4:+59876895 Msa0860800:CDS
GCAAAATGTTGATGAGCAAA+AGG 0.428473 5_4:+59877221 Msa0860800:CDS
CGATGGTTCCTTTGGTCTTG+GGG 0.432437 5_4:-59877088 Msa0860810:three_prime_UTR
GCAAAAGGTTGATGAGCAAA+AGG 0.439413 5_4:+59877110 Msa0860800:CDS
TTGCCTCTTAGTAATTGAAA+TGG 0.441701 5_4:-59876464 None:intergenic
CATGCTAATCTTATTGGTCG+TGG 0.455442 5_4:+59877710 Msa0860800:CDS
ACTTTGAACCATATCATGAA+TGG 0.461998 5_4:+59876572 Msa0860800:CDS
CATGAATGGGATAAATATGA+TGG 0.465682 5_4:+59876586 Msa0860800:CDS
TCTTTCACCTATCAGCCTTA+GGG 0.470253 5_4:-59876902 None:intergenic
TCAAAAGATACCACCACAAA+AGG 0.472076 5_4:+59877422 Msa0860800:CDS
TCTTAAGAAGTGGAAGAGAT+TGG 0.472099 5_4:+59877677 Msa0860800:CDS
GGTCTTGGGGCTTCTTCTGG+TGG 0.474159 5_4:-59877075 Msa0860810:three_prime_UTR
GCGGAGAAAGTGAGGACTAA+TGG 0.476427 5_4:+59877498 Msa0860800:CDS
CGTTTCACTGTTATGCTACC+AGG 0.481775 5_4:+59876610 Msa0860800:CDS
TGCTAAACTTACTAATCCTA+AGG 0.484970 5_4:+59877038 Msa0860800:CDS
TCTTATTGGTCGTGGGACTT+GGG 0.489096 5_4:+59877718 Msa0860800:CDS
CAGGATGAAAATGAAGCCCT+TGG 0.494161 5_4:+59877639 Msa0860800:CDS
ACCAAAGGAATCGCCACAAA+AGG 0.496416 5_4:+59877317 Msa0860800:CDS
ACCAAAGGAACCATCGCAAA+AGG 0.505295 5_4:+59877095 Msa0860800:CDS
TGACCATTTCAATTACTAAG+AGG 0.508482 5_4:+59876461 Msa0860800:five_prime_UTR
CTTTGAACCATATCATGAAT+GGG 0.508732 5_4:+59876573 Msa0860800:CDS
ATGCTAATCTTATTGGTCGT+GGG 0.509173 5_4:+59877711 Msa0860800:CDS
TTATTGCTAGATGCAAAACC+AGG 0.528675 5_4:+59877580 Msa0860800:CDS
AGAAGAGAAAGTAGCAACAA+AGG 0.529245 5_4:+59877149 Msa0860800:CDS
TTTGCATCTAGCAATAAACT+TGG 0.529793 5_4:-59877574 Msa0860810:three_prime_UTR
GTGTCATAATCAGATGGAAT+GGG 0.536982 5_4:-59876967 None:intergenic
TCACCTATCAGCCTTAGGGC+AGG 0.538165 5_4:-59876898 None:intergenic
GCCCTTGGAAATTCGACCAC+AGG 0.540651 5_4:+59877654 Msa0860800:CDS
GGTCTTGGAGCTTCTTCTGG+TGG 0.541290 5_4:-59877186 Msa0860810:three_prime_UTR
GGTCTTGGAGCTTCTTCTGG+TGG 0.541290 5_4:-59877297 Msa0860810:three_prime_UTR
GAGAGTGAAATCTACAAGCC+TGG 0.545424 5_4:-59877598 Msa0860810:three_prime_UTR
CAAAATGTTGATGAGCAAAA+GGG 0.546557 5_4:+59877222 Msa0860800:CDS
CAAATATTAGTACATCAGTA+AGG 0.547214 5_4:-59877807 Msa0860810:three_prime_UTR
CAAAAGGCTGATGAGCAAAA+GGG 0.548506 5_4:+59877333 Msa0860800:CDS
CAGTGTGACTGCAACATTTG+AGG 0.549500 5_4:+59876993 Msa0860800:CDS
CGCCATCTGTTCTGAAAGGT+CGG 0.552867 5_4:-59876925 None:intergenic
CAAAAGGTTGATGAGCAAAA+GGG 0.552944 5_4:+59877111 Msa0860800:CDS
AGTGTGACTGCAACATTTGA+GGG 0.554937 5_4:+59876994 Msa0860800:CDS
GAAATAATAACAGCTCTTCG+AGG 0.555655 5_4:-59877785 Msa0860810:three_prime_UTR
GACCTGTGGTCGAATTTCCA+AGG 0.558465 5_4:-59877656 Msa0860810:three_prime_UTR
AGAAGAAGCCCCAAGACCAA+AGG 0.559386 5_4:+59877080 Msa0860800:CDS
AGAAGAGAAAGCAGAAACAA+AGG 0.563791 5_4:+59877260 Msa0860800:CDS
CGTGTCATAATCAGATGGAA+TGG 0.580612 5_4:-59876968 None:intergenic
TGACATCAAAACCTGCCCTA+AGG 0.581927 5_4:+59876887 Msa0860800:CDS
TAAAAGCAAAAGAGAAACAA+AGG 0.585363 5_4:+59876494 Msa0860800:five_prime_UTR
GACCGACCTTTCAGAACAGA+TGG 0.586897 5_4:+59876923 Msa0860800:CDS
ATATAGAAGGGATCAAATGA+AGG 0.587427 5_4:+59876858 Msa0860800:CDS
AATACATTTGGATCGTCTCA+GGG 0.587906 5_4:+59877753 Msa0860800:CDS
TGAGCAAAAGGGTGCTCAAG+AGG 0.590093 5_4:+59877344 Msa0860800:CDS
ACCTGTGGTCGAATTTCCAA+GGG 0.591551 5_4:-59877655 Msa0860810:three_prime_UTR
TTATCATGCAGGATATAGAA+GGG 0.591949 5_4:+59876846 Msa0860800:intron
CGACCACAGGTCTTAAGAAG+TGG 0.593414 5_4:+59877667 Msa0860800:CDS
ATGAATGGGATAAATATGAT+GGG 0.593539 5_4:+59876587 Msa0860800:CDS
ACAAAGTGGCGGAGAAAGTG+AGG 0.597287 5_4:+59877490 Msa0860800:CDS
GAAAGTGAGGACTAATGGCT+CGG 0.599305 5_4:+59877503 Msa0860800:CDS
GAGCAAAAGGGTGCTCAAGA+GGG 0.603253 5_4:+59877345 Msa0860800:CDS
CTTCCACTTCTTAAGACCTG+TGG 0.604468 5_4:-59877670 Msa0860810:three_prime_UTR
AGAAGAAGCTCCAAGACCAA+AGG 0.617008 5_4:+59877191 Msa0860800:CDS
CATTCCATCTGATTATGACA+CGG 0.620370 5_4:+59876969 Msa0860800:CDS
ACGACGCCATCTGTTCTGAA+AGG 0.626270 5_4:-59876929 None:intergenic
CTGTCCGTGTCATAATCAGA+TGG 0.633670 5_4:-59876973 None:intergenic
AGAAGAAGCTCCAAGACCAA+AGG 0.639663 5_4:+59877302 Msa0860800:CDS
AGAAGCTAGCATAGACAAAG+TGG 0.640905 5_4:+59877476 Msa0860800:CDS
AGCTAGCATAGACAAAGTGG+CGG 0.665250 5_4:+59877479 Msa0860800:CDS
TAATTGAAATGGTCATGACA+TGG 0.727826 5_4:-59876453 None:intergenic
GTGACTGCAACATTTGAGGG+TGG 0.744026 5_4:+59876997 Msa0860800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAAAAATGTATATTATT+TGG - chr5_4:59876695-59876714 None:intergenic 10.0%
!! GTAAAAAAATGTATATTATT+TGG - chr5_4:59876695-59876714 None:intergenic 10.0%
!!! TATTTCAAGTTTTTATATCT+TGG + chr5_4:59876640-59876659 Msa0860800:intron 15.0%
!!! ATATACATTTTTTTACTGTA+AGG + chr5_4:59876699-59876718 Msa0860800:intron 15.0%
!!! TATTTCAAGTTTTTATATCT+TGG + chr5_4:59876640-59876659 Msa0860800:intron 15.0%
!!! ATATACATTTTTTTACTGTA+AGG + chr5_4:59876699-59876718 Msa0860800:intron 15.0%
!!! ACAAAGGCATAAATAATAAA+TGG + chr5_4:59876510-59876529 Msa0860800:exon 20.0%
!!! AAAACAGAGCTTTTTATAAA+TGG - chr5_4:59876757-59876776 None:intergenic 20.0%
!!! TCTGTTTTTCATTAATTTGA+AGG + chr5_4:59876769-59876788 Msa0860800:intron 20.0%
!!! AGTATATAGTTCGTATTTTT+TGG - chr5_4:59876823-59876842 None:intergenic 20.0%
!! CTTTATTGTAGAAATACATT+TGG + chr5_4:59877741-59877760 Msa0860800:CDS 20.0%
!!! ACAAAGGCATAAATAATAAA+TGG + chr5_4:59876510-59876529 Msa0860800:exon 20.0%
!!! AAAACAGAGCTTTTTATAAA+TGG - chr5_4:59876757-59876776 None:intergenic 20.0%
!!! TCTGTTTTTCATTAATTTGA+AGG + chr5_4:59876769-59876788 Msa0860800:intron 20.0%
!!! AGTATATAGTTCGTATTTTT+TGG - chr5_4:59876823-59876842 None:intergenic 20.0%
!! CTTTATTGTAGAAATACATT+TGG + chr5_4:59877741-59877760 Msa0860800:CDS 20.0%
! TAAAAGCAAAAGAGAAACAA+AGG + chr5_4:59876494-59876513 Msa0860800:five_prime_UTR 25.0%
! TATTTATCCCATTCATGATA+TGG - chr5_4:59876583-59876602 None:intergenic 25.0%
! ATGAATGGGATAAATATGAT+GGG + chr5_4:59876587-59876606 Msa0860800:CDS 25.0%
! AACTTGAAATAACATTTACC+TGG - chr5_4:59876631-59876650 None:intergenic 25.0%
! TATATACTTTCTTATCATGC+AGG + chr5_4:59876835-59876854 Msa0860800:intron 25.0%
!! ATTGTATTATTCTCCTTTTG+TGG - chr5_4:59877438-59877457 Msa0860810:three_prime_UTR 25.0%
! TAAAAGCAAAAGAGAAACAA+AGG + chr5_4:59876494-59876513 Msa0860800:five_prime_UTR 25.0%
! TATTTATCCCATTCATGATA+TGG - chr5_4:59876583-59876602 None:intergenic 25.0%
! ATGAATGGGATAAATATGAT+GGG + chr5_4:59876587-59876606 Msa0860800:CDS 25.0%
! AACTTGAAATAACATTTACC+TGG - chr5_4:59876631-59876650 None:intergenic 25.0%
! TATATACTTTCTTATCATGC+AGG + chr5_4:59876835-59876854 Msa0860800:intron 25.0%
!! ATTGTATTATTCTCCTTTTG+TGG - chr5_4:59877438-59877457 Msa0860810:three_prime_UTR 25.0%
TAATTGAAATGGTCATGACA+TGG - chr5_4:59876456-59876475 None:intergenic 30.0%
TGACCATTTCAATTACTAAG+AGG + chr5_4:59876461-59876480 Msa0860800:five_prime_UTR 30.0%
TTGCCTCTTAGTAATTGAAA+TGG - chr5_4:59876467-59876486 None:intergenic 30.0%
ACTTTGAACCATATCATGAA+TGG + chr5_4:59876572-59876591 Msa0860800:CDS 30.0%
CTTTGAACCATATCATGAAT+GGG + chr5_4:59876573-59876592 Msa0860800:CDS 30.0%
CATGAATGGGATAAATATGA+TGG + chr5_4:59876586-59876605 Msa0860800:CDS 30.0%
TTATCATGCAGGATATAGAA+GGG + chr5_4:59876846-59876865 Msa0860800:intron 30.0%
ATATAGAAGGGATCAAATGA+AGG + chr5_4:59876858-59876877 Msa0860800:CDS 30.0%
TGCTAAACTTACTAATCCTA+AGG + chr5_4:59877038-59877057 Msa0860800:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_4:59877139-59877158 Msa0860810:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_4:59877140-59877159 Msa0860810:three_prime_UTR 30.0%
!!! ACTTTCTCTTCTTTAGCTTT+TGG - chr5_4:59877141-59877160 Msa0860810:three_prime_UTR 30.0%
! CAAAATGTTGATGAGCAAAA+GGG + chr5_4:59877222-59877241 Msa0860800:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_4:59877139-59877158 Msa0860810:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_4:59877140-59877159 Msa0860810:three_prime_UTR 30.0%
! TTTCTCTTCTATAGCTTTTG+GGG - chr5_4:59877373-59877392 Msa0860810:three_prime_UTR 30.0%
! CTTTCTCTTCTATAGCTTTT+GGG - chr5_4:59877374-59877393 Msa0860810:three_prime_UTR 30.0%
TTTGCATCTAGCAATAAACT+TGG - chr5_4:59877577-59877596 Msa0860810:three_prime_UTR 30.0%
ATGTGTCATGCTAATCTTAT+TGG + chr5_4:59877704-59877723 Msa0860800:CDS 30.0%
TAATTGAAATGGTCATGACA+TGG - chr5_4:59876456-59876475 None:intergenic 30.0%
TGACCATTTCAATTACTAAG+AGG + chr5_4:59876461-59876480 Msa0860800:five_prime_UTR 30.0%
TTGCCTCTTAGTAATTGAAA+TGG - chr5_4:59876467-59876486 None:intergenic 30.0%
ACTTTGAACCATATCATGAA+TGG + chr5_4:59876572-59876591 Msa0860800:CDS 30.0%
CTTTGAACCATATCATGAAT+GGG + chr5_4:59876573-59876592 Msa0860800:CDS 30.0%
CATGAATGGGATAAATATGA+TGG + chr5_4:59876586-59876605 Msa0860800:CDS 30.0%
TTATCATGCAGGATATAGAA+GGG + chr5_4:59876846-59876865 Msa0860800:intron 30.0%
ATATAGAAGGGATCAAATGA+AGG + chr5_4:59876858-59876877 Msa0860800:CDS 30.0%
TGCTAAACTTACTAATCCTA+AGG + chr5_4:59877038-59877057 Msa0860800:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_4:59877139-59877158 Msa0860810:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_4:59877140-59877159 Msa0860810:three_prime_UTR 30.0%
!!! ACTTTCTCTTCTTTAGCTTT+TGG - chr5_4:59877141-59877160 Msa0860810:three_prime_UTR 30.0%
! CAAAATGTTGATGAGCAAAA+GGG + chr5_4:59877222-59877241 Msa0860800:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_4:59877139-59877158 Msa0860810:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_4:59877140-59877159 Msa0860810:three_prime_UTR 30.0%
! TTTCTCTTCTATAGCTTTTG+GGG - chr5_4:59877373-59877392 Msa0860810:three_prime_UTR 30.0%
! CTTTCTCTTCTATAGCTTTT+GGG - chr5_4:59877374-59877393 Msa0860810:three_prime_UTR 30.0%
TTTGCATCTAGCAATAAACT+TGG - chr5_4:59877577-59877596 Msa0860810:three_prime_UTR 30.0%
ATGTGTCATGCTAATCTTAT+TGG + chr5_4:59877704-59877723 Msa0860800:CDS 30.0%
CTTATCATGCAGGATATAGA+AGG + chr5_4:59876845-59876864 Msa0860800:intron 35.0%
GTGTCATAATCAGATGGAAT+GGG - chr5_4:59876970-59876989 None:intergenic 35.0%
CATTCCATCTGATTATGACA+CGG + chr5_4:59876969-59876988 Msa0860800:CDS 35.0%
!! GGATTTGTTGTTGTTTCCTT+AGG - chr5_4:59877057-59877076 Msa0860810:three_prime_UTR 35.0%
!! CAAAAGGTTGATGAGCAAAA+GGG + chr5_4:59877111-59877130 Msa0860800:CDS 35.0%
AGAAGAGAAAGTAGCAACAA+AGG + chr5_4:59877149-59877168 Msa0860800:CDS 35.0%
! GCAAAATGTTGATGAGCAAA+AGG + chr5_4:59877221-59877240 Msa0860800:CDS 35.0%
!!! GCTTTCTCTTCTTTAGCTTT+TGG - chr5_4:59877252-59877271 Msa0860810:three_prime_UTR 35.0%
AGAAGAGAAAGCAGAAACAA+AGG + chr5_4:59877260-59877279 Msa0860800:CDS 35.0%
! GCTTTCTCTTCTATAGCTTT+TGG - chr5_4:59877375-59877394 Msa0860810:three_prime_UTR 35.0%
! TTGTGGTGGTATCTTTTGAT+CGG - chr5_4:59877421-59877440 Msa0860810:three_prime_UTR 35.0%
TCAAAAGATACCACCACAAA+AGG + chr5_4:59877422-59877441 Msa0860800:CDS 35.0%
! GTATTATTCTCCTTTTGTGG+TGG - chr5_4:59877435-59877454 Msa0860810:three_prime_UTR 35.0%
!!! AACTTGGCATCTTTGGTTTT+AGG - chr5_4:59877561-59877580 Msa0860810:three_prime_UTR 35.0%
AGCAATAAACTTGGCATCTT+TGG - chr5_4:59877568-59877587 Msa0860810:three_prime_UTR 35.0%
TTATTGCTAGATGCAAAACC+AGG + chr5_4:59877580-59877599 Msa0860800:CDS 35.0%
TGTAGATTTCACTCTCTCTA+AGG + chr5_4:59877605-59877624 Msa0860800:CDS 35.0%
TCTTAAGAAGTGGAAGAGAT+TGG + chr5_4:59877677-59877696 Msa0860800:CDS 35.0%
ATGCTAATCTTATTGGTCGT+GGG + chr5_4:59877711-59877730 Msa0860800:CDS 35.0%
AAATACATTTGGATCGTCTC+AGG + chr5_4:59877752-59877771 Msa0860800:CDS 35.0%
AATACATTTGGATCGTCTCA+GGG + chr5_4:59877753-59877772 Msa0860800:CDS 35.0%
GAAATAATAACAGCTCTTCG+AGG - chr5_4:59877788-59877807 Msa0860810:three_prime_UTR 35.0%
CTTATCATGCAGGATATAGA+AGG + chr5_4:59876845-59876864 Msa0860800:intron 35.0%
GTGTCATAATCAGATGGAAT+GGG - chr5_4:59876970-59876989 None:intergenic 35.0%
CATTCCATCTGATTATGACA+CGG + chr5_4:59876969-59876988 Msa0860800:CDS 35.0%
!! GGATTTGTTGTTGTTTCCTT+AGG - chr5_4:59877057-59877076 Msa0860810:three_prime_UTR 35.0%
!! CAAAAGGTTGATGAGCAAAA+GGG + chr5_4:59877111-59877130 Msa0860800:CDS 35.0%
AGAAGAGAAAGTAGCAACAA+AGG + chr5_4:59877149-59877168 Msa0860800:CDS 35.0%
! GCAAAATGTTGATGAGCAAA+AGG + chr5_4:59877221-59877240 Msa0860800:CDS 35.0%
!!! GCTTTCTCTTCTTTAGCTTT+TGG - chr5_4:59877252-59877271 Msa0860810:three_prime_UTR 35.0%
AGAAGAGAAAGCAGAAACAA+AGG + chr5_4:59877260-59877279 Msa0860800:CDS 35.0%
! GCTTTCTCTTCTATAGCTTT+TGG - chr5_4:59877375-59877394 Msa0860810:three_prime_UTR 35.0%
! TTGTGGTGGTATCTTTTGAT+CGG - chr5_4:59877421-59877440 Msa0860810:three_prime_UTR 35.0%
TCAAAAGATACCACCACAAA+AGG + chr5_4:59877422-59877441 Msa0860800:CDS 35.0%
! GTATTATTCTCCTTTTGTGG+TGG - chr5_4:59877435-59877454 Msa0860810:three_prime_UTR 35.0%
!!! AACTTGGCATCTTTGGTTTT+AGG - chr5_4:59877561-59877580 Msa0860810:three_prime_UTR 35.0%
AGCAATAAACTTGGCATCTT+TGG - chr5_4:59877568-59877587 Msa0860810:three_prime_UTR 35.0%
TTATTGCTAGATGCAAAACC+AGG + chr5_4:59877580-59877599 Msa0860800:CDS 35.0%
TGTAGATTTCACTCTCTCTA+AGG + chr5_4:59877605-59877624 Msa0860800:CDS 35.0%
TCTTAAGAAGTGGAAGAGAT+TGG + chr5_4:59877677-59877696 Msa0860800:CDS 35.0%
ATGCTAATCTTATTGGTCGT+GGG + chr5_4:59877711-59877730 Msa0860800:CDS 35.0%
AAATACATTTGGATCGTCTC+AGG + chr5_4:59877752-59877771 Msa0860800:CDS 35.0%
AATACATTTGGATCGTCTCA+GGG + chr5_4:59877753-59877772 Msa0860800:CDS 35.0%
GAAATAATAACAGCTCTTCG+AGG - chr5_4:59877788-59877807 Msa0860810:three_prime_UTR 35.0%
TCTTTCACCTATCAGCCTTA+GGG - chr5_4:59876905-59876924 None:intergenic 40.0%
CGTGTCATAATCAGATGGAA+TGG - chr5_4:59876971-59876990 None:intergenic 40.0%
AGTGTGACTGCAACATTTGA+GGG + chr5_4:59876994-59877013 Msa0860800:CDS 40.0%
!! GCAAAAGGTTGATGAGCAAA+AGG + chr5_4:59877110-59877129 Msa0860800:CDS 40.0%
! ACATTTTGCGATGGTTCCTT+TGG - chr5_4:59877210-59877229 Msa0860810:three_prime_UTR 40.0%
! TGCTCATCAACATTTTGCGA+TGG - chr5_4:59877219-59877238 Msa0860810:three_prime_UTR 40.0%
! TTTTGCTCATCAGCCTTTTG+TGG - chr5_4:59877333-59877352 Msa0860810:three_prime_UTR 40.0%
ACAAAAGGCTGATGAGCAAA+AGG + chr5_4:59877332-59877351 Msa0860800:CDS 40.0%
CAAAAGGCTGATGAGCAAAA+GGG + chr5_4:59877333-59877352 Msa0860800:CDS 40.0%
! AGCTTCTGTTCTGCTCTTTT+CGG - chr5_4:59877463-59877482 Msa0860810:three_prime_UTR 40.0%
AGAAGCTAGCATAGACAAAG+TGG + chr5_4:59877476-59877495 Msa0860800:CDS 40.0%
CATGCTAATCTTATTGGTCG+TGG + chr5_4:59877710-59877729 Msa0860800:CDS 40.0%
TCTTTCACCTATCAGCCTTA+GGG - chr5_4:59876905-59876924 None:intergenic 40.0%
CGTGTCATAATCAGATGGAA+TGG - chr5_4:59876971-59876990 None:intergenic 40.0%
AGTGTGACTGCAACATTTGA+GGG + chr5_4:59876994-59877013 Msa0860800:CDS 40.0%
!! GCAAAAGGTTGATGAGCAAA+AGG + chr5_4:59877110-59877129 Msa0860800:CDS 40.0%
! ACATTTTGCGATGGTTCCTT+TGG - chr5_4:59877210-59877229 Msa0860810:three_prime_UTR 40.0%
! TGCTCATCAACATTTTGCGA+TGG - chr5_4:59877219-59877238 Msa0860810:three_prime_UTR 40.0%
! TTTTGCTCATCAGCCTTTTG+TGG - chr5_4:59877333-59877352 Msa0860810:three_prime_UTR 40.0%
ACAAAAGGCTGATGAGCAAA+AGG + chr5_4:59877332-59877351 Msa0860800:CDS 40.0%
CAAAAGGCTGATGAGCAAAA+GGG + chr5_4:59877333-59877352 Msa0860800:CDS 40.0%
! AGCTTCTGTTCTGCTCTTTT+CGG - chr5_4:59877463-59877482 Msa0860810:three_prime_UTR 40.0%
AGAAGCTAGCATAGACAAAG+TGG + chr5_4:59877476-59877495 Msa0860800:CDS 40.0%
CATGCTAATCTTATTGGTCG+TGG + chr5_4:59877710-59877729 Msa0860800:CDS 40.0%
TCATAGACACGATCAGCTTC+TGG - chr5_4:59876550-59876569 None:intergenic 45.0%
CGTTTCACTGTTATGCTACC+AGG + chr5_4:59876610-59876629 Msa0860800:CDS 45.0%
TGACATCAAAACCTGCCCTA+AGG + chr5_4:59876887-59876906 Msa0860800:CDS 45.0%
GTCTTTCACCTATCAGCCTT+AGG - chr5_4:59876906-59876925 None:intergenic 45.0%
CTGTCCGTGTCATAATCAGA+TGG - chr5_4:59876976-59876995 None:intergenic 45.0%
CAGTGTGACTGCAACATTTG+AGG + chr5_4:59876993-59877012 Msa0860800:CDS 45.0%
ACCAAAGGAACCATCGCAAA+AGG + chr5_4:59877095-59877114 Msa0860800:CDS 45.0%
! ACCTTTTGCGATGGTTCCTT+TGG - chr5_4:59877099-59877118 Msa0860810:three_prime_UTR 45.0%
! TGCTCATCAACCTTTTGCGA+TGG - chr5_4:59877108-59877127 Msa0860810:three_prime_UTR 45.0%
!! TTTGGTCTTGGAGCTTCTTC+TGG - chr5_4:59877192-59877211 Msa0860810:three_prime_UTR 45.0%
AGAAGAAGCTCCAAGACCAA+AGG + chr5_4:59877191-59877210 Msa0860800:CDS 45.0%
!! TTTGGTCTTGGAGCTTCTTC+TGG - chr5_4:59877192-59877211 Msa0860810:three_prime_UTR 45.0%
AGAAGAAGCTCCAAGACCAA+AGG + chr5_4:59877191-59877210 Msa0860800:CDS 45.0%
ACCAAAGGAATCGCCACAAA+AGG + chr5_4:59877317-59877336 Msa0860800:CDS 45.0%
AGCTAGCATAGACAAAGTGG+CGG + chr5_4:59877479-59877498 Msa0860800:CDS 45.0%
GAAAGTGAGGACTAATGGCT+CGG + chr5_4:59877503-59877522 Msa0860800:CDS 45.0%
GAGAGTGAAATCTACAAGCC+TGG - chr5_4:59877601-59877620 Msa0860810:three_prime_UTR 45.0%
!! CTCTAAGGCTCTCTGTAATC+AGG + chr5_4:59877620-59877639 Msa0860800:CDS 45.0%
CAGGATGAAAATGAAGCCCT+TGG + chr5_4:59877639-59877658 Msa0860800:CDS 45.0%
ACCTGTGGTCGAATTTCCAA+GGG - chr5_4:59877658-59877677 Msa0860810:three_prime_UTR 45.0%
CTTCCACTTCTTAAGACCTG+TGG - chr5_4:59877673-59877692 Msa0860810:three_prime_UTR 45.0%
ATCTTATTGGTCGTGGGACT+TGG + chr5_4:59877717-59877736 Msa0860800:CDS 45.0%
TCTTATTGGTCGTGGGACTT+GGG + chr5_4:59877718-59877737 Msa0860800:CDS 45.0%
TCATAGACACGATCAGCTTC+TGG - chr5_4:59876550-59876569 None:intergenic 45.0%
CGTTTCACTGTTATGCTACC+AGG + chr5_4:59876610-59876629 Msa0860800:CDS 45.0%
TGACATCAAAACCTGCCCTA+AGG + chr5_4:59876887-59876906 Msa0860800:CDS 45.0%
GTCTTTCACCTATCAGCCTT+AGG - chr5_4:59876906-59876925 None:intergenic 45.0%
CTGTCCGTGTCATAATCAGA+TGG - chr5_4:59876976-59876995 None:intergenic 45.0%
CAGTGTGACTGCAACATTTG+AGG + chr5_4:59876993-59877012 Msa0860800:CDS 45.0%
ACCAAAGGAACCATCGCAAA+AGG + chr5_4:59877095-59877114 Msa0860800:CDS 45.0%
! ACCTTTTGCGATGGTTCCTT+TGG - chr5_4:59877099-59877118 Msa0860810:three_prime_UTR 45.0%
! TGCTCATCAACCTTTTGCGA+TGG - chr5_4:59877108-59877127 Msa0860810:three_prime_UTR 45.0%
!! TTTGGTCTTGGAGCTTCTTC+TGG - chr5_4:59877192-59877211 Msa0860810:three_prime_UTR 45.0%
AGAAGAAGCTCCAAGACCAA+AGG + chr5_4:59877191-59877210 Msa0860800:CDS 45.0%
!! TTTGGTCTTGGAGCTTCTTC+TGG - chr5_4:59877192-59877211 Msa0860810:three_prime_UTR 45.0%
AGAAGAAGCTCCAAGACCAA+AGG + chr5_4:59877191-59877210 Msa0860800:CDS 45.0%
ACCAAAGGAATCGCCACAAA+AGG + chr5_4:59877317-59877336 Msa0860800:CDS 45.0%
AGCTAGCATAGACAAAGTGG+CGG + chr5_4:59877479-59877498 Msa0860800:CDS 45.0%
GAAAGTGAGGACTAATGGCT+CGG + chr5_4:59877503-59877522 Msa0860800:CDS 45.0%
GAGAGTGAAATCTACAAGCC+TGG - chr5_4:59877601-59877620 Msa0860810:three_prime_UTR 45.0%
!! CTCTAAGGCTCTCTGTAATC+AGG + chr5_4:59877620-59877639 Msa0860800:CDS 45.0%
CAGGATGAAAATGAAGCCCT+TGG + chr5_4:59877639-59877658 Msa0860800:CDS 45.0%
ACCTGTGGTCGAATTTCCAA+GGG - chr5_4:59877658-59877677 Msa0860810:three_prime_UTR 45.0%
CTTCCACTTCTTAAGACCTG+TGG - chr5_4:59877673-59877692 Msa0860810:three_prime_UTR 45.0%
ATCTTATTGGTCGTGGGACT+TGG + chr5_4:59877717-59877736 Msa0860800:CDS 45.0%
TCTTATTGGTCGTGGGACTT+GGG + chr5_4:59877718-59877737 Msa0860800:CDS 45.0%
!! TAAAAAAATGTATATTATTT+GGG - chr5_4:59876694-59876713 None:intergenic 5.0%
!! TAAAAAAATGTATATTATTT+GGG - chr5_4:59876694-59876713 None:intergenic 5.0%
AAACCTGCCCTAAGGCTGAT+AGG + chr5_4:59876895-59876914 Msa0860800:CDS 50.0%
GACCGACCTTTCAGAACAGA+TGG + chr5_4:59876923-59876942 Msa0860800:CDS 50.0%
!! CGCCATCTGTTCTGAAAGGT+CGG - chr5_4:59876928-59876947 None:intergenic 50.0%
! ACGACGCCATCTGTTCTGAA+AGG - chr5_4:59876932-59876951 None:intergenic 50.0%
GTGACTGCAACATTTGAGGG+TGG + chr5_4:59876997-59877016 Msa0860800:CDS 50.0%
!! TTTGGTCTTGGGGCTTCTTC+TGG - chr5_4:59877081-59877100 Msa0860810:three_prime_UTR 50.0%
AGAAGAAGCCCCAAGACCAA+AGG + chr5_4:59877080-59877099 Msa0860800:CDS 50.0%
!! CGATGGTTCCTTTGGTCTTG+GGG - chr5_4:59877091-59877110 Msa0860810:three_prime_UTR 50.0%
! GCGATGGTTCCTTTGGTCTT+GGG - chr5_4:59877092-59877111 Msa0860810:three_prime_UTR 50.0%
TGCGATGGTTCCTTTGGTCT+TGG - chr5_4:59877093-59877112 Msa0860810:three_prime_UTR 50.0%
TGCGATGGTTCCTTTGGTCT+TGG - chr5_4:59877093-59877112 Msa0860810:three_prime_UTR 50.0%
TGTGGCGATTCCTTTGGTCT+TGG - chr5_4:59877315-59877334 Msa0860810:three_prime_UTR 50.0%
! GCCTTTTGTGGCGATTCCTT+TGG - chr5_4:59877321-59877340 Msa0860810:three_prime_UTR 50.0%
!! TGAGCAAAAGGGTGCTCAAG+AGG + chr5_4:59877344-59877363 Msa0860800:CDS 50.0%
!! GAGCAAAAGGGTGCTCAAGA+GGG + chr5_4:59877345-59877364 Msa0860800:CDS 50.0%
! ACAAAGTGGCGGAGAAAGTG+AGG + chr5_4:59877490-59877509 Msa0860800:CDS 50.0%
GCGGAGAAAGTGAGGACTAA+TGG + chr5_4:59877498-59877517 Msa0860800:CDS 50.0%
GACCTGTGGTCGAATTTCCA+AGG - chr5_4:59877659-59877678 Msa0860810:three_prime_UTR 50.0%
CGACCACAGGTCTTAAGAAG+TGG + chr5_4:59877667-59877686 Msa0860800:CDS 50.0%
TGGATCGTCTCAGGGAGAAT+TGG + chr5_4:59877761-59877780 Msa0860800:CDS 50.0%
AAACCTGCCCTAAGGCTGAT+AGG + chr5_4:59876895-59876914 Msa0860800:CDS 50.0%
GACCGACCTTTCAGAACAGA+TGG + chr5_4:59876923-59876942 Msa0860800:CDS 50.0%
!! CGCCATCTGTTCTGAAAGGT+CGG - chr5_4:59876928-59876947 None:intergenic 50.0%
! ACGACGCCATCTGTTCTGAA+AGG - chr5_4:59876932-59876951 None:intergenic 50.0%
GTGACTGCAACATTTGAGGG+TGG + chr5_4:59876997-59877016 Msa0860800:CDS 50.0%
!! TTTGGTCTTGGGGCTTCTTC+TGG - chr5_4:59877081-59877100 Msa0860810:three_prime_UTR 50.0%
AGAAGAAGCCCCAAGACCAA+AGG + chr5_4:59877080-59877099 Msa0860800:CDS 50.0%
!! CGATGGTTCCTTTGGTCTTG+GGG - chr5_4:59877091-59877110 Msa0860810:three_prime_UTR 50.0%
! GCGATGGTTCCTTTGGTCTT+GGG - chr5_4:59877092-59877111 Msa0860810:three_prime_UTR 50.0%
TGCGATGGTTCCTTTGGTCT+TGG - chr5_4:59877093-59877112 Msa0860810:three_prime_UTR 50.0%
TGCGATGGTTCCTTTGGTCT+TGG - chr5_4:59877093-59877112 Msa0860810:three_prime_UTR 50.0%
TGTGGCGATTCCTTTGGTCT+TGG - chr5_4:59877315-59877334 Msa0860810:three_prime_UTR 50.0%
! GCCTTTTGTGGCGATTCCTT+TGG - chr5_4:59877321-59877340 Msa0860810:three_prime_UTR 50.0%
!! TGAGCAAAAGGGTGCTCAAG+AGG + chr5_4:59877344-59877363 Msa0860800:CDS 50.0%
!! GAGCAAAAGGGTGCTCAAGA+GGG + chr5_4:59877345-59877364 Msa0860800:CDS 50.0%
! ACAAAGTGGCGGAGAAAGTG+AGG + chr5_4:59877490-59877509 Msa0860800:CDS 50.0%
GCGGAGAAAGTGAGGACTAA+TGG + chr5_4:59877498-59877517 Msa0860800:CDS 50.0%
GACCTGTGGTCGAATTTCCA+AGG - chr5_4:59877659-59877678 Msa0860810:three_prime_UTR 50.0%
CGACCACAGGTCTTAAGAAG+TGG + chr5_4:59877667-59877686 Msa0860800:CDS 50.0%
TGGATCGTCTCAGGGAGAAT+TGG + chr5_4:59877761-59877780 Msa0860800:CDS 50.0%
TCACCTATCAGCCTTAGGGC+AGG - chr5_4:59876901-59876920 None:intergenic 55.0%
GGTCTTGGAGCTTCTTCTGG+TGG - chr5_4:59877189-59877208 Msa0860810:three_prime_UTR 55.0%
GGTCTTGGAGCTTCTTCTGG+TGG - chr5_4:59877189-59877208 Msa0860810:three_prime_UTR 55.0%
GCCCTTGGAAATTCGACCAC+AGG + chr5_4:59877654-59877673 Msa0860800:CDS 55.0%
TCACCTATCAGCCTTAGGGC+AGG - chr5_4:59876901-59876920 None:intergenic 55.0%
GGTCTTGGAGCTTCTTCTGG+TGG - chr5_4:59877189-59877208 Msa0860810:three_prime_UTR 55.0%
GGTCTTGGAGCTTCTTCTGG+TGG - chr5_4:59877189-59877208 Msa0860810:three_prime_UTR 55.0%
GCCCTTGGAAATTCGACCAC+AGG + chr5_4:59877654-59877673 Msa0860800:CDS 55.0%
GGTCTTGGGGCTTCTTCTGG+TGG - chr5_4:59877078-59877097 Msa0860810:three_prime_UTR 60.0%
GGTCTTGGGGCTTCTTCTGG+TGG - chr5_4:59877078-59877097 Msa0860810:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 59876386 59877815 59876386 ID=Msa0860800;Name=Msa0860800
chr5_4 mRNA 59876386 59877815 59876386 ID=Msa0860800-mRNA-1;Parent=Msa0860800;Name=Msa0860800-mRNA-1;_AED=0.23;_eAED=0.23;_QI=143|1|0.5|1|1|1|2|0|353
chr5_4 exon 59876386 59876631 59876386 ID=Msa0860800-mRNA-1:exon:18393;Parent=Msa0860800-mRNA-1
chr5_4 exon 59876857 59877815 59876857 ID=Msa0860800-mRNA-1:exon:18394;Parent=Msa0860800-mRNA-1
chr5_4 five_prime_UTR 59876386 59876528 59876386 ID=Msa0860800-mRNA-1:five_prime_utr;Parent=Msa0860800-mRNA-1
chr5_4 CDS 59876529 59876631 59876529 ID=Msa0860800-mRNA-1:cds;Parent=Msa0860800-mRNA-1
chr5_4 CDS 59876857 59877815 59876857 ID=Msa0860800-mRNA-1:cds;Parent=Msa0860800-mRNA-1
Gene Sequence

>Msa0860800

ATGGATACAAAGACACAGCCAGAAGCTGATCGTGTCTATGAAGACTTTGAACCATATCATGAATGGGATAAATATGATGGGCGTTTCACTGTTATGCTACCAGGATATAGAAGGGATCAAATGAAGGTTCAAGTGACATCAAAACCTGCCCTAAGGCTGATAGGTGAAAGACCGACCTTTCAGAACAGATGGCGTCGTTTTAAATTAGAATTTCCCATTCCATCTGATTATGACACGGACAGTGTGACTGCAACATTTGAGGGTGGCAAGCTATCTGTCAAATTTGCTAAACTTACTAATCCTAAGGAAACAACAACAAATCCACCAGAAGAAGCCCCAAGACCAAAGGAACCATCGCAAAAGGTTGATGAGCAAAAGGGTGCCCCAAAAGCTAAAGAAGAGAAAGTAGCAACAAAGGAAACAACAACAAATCCACCAGAAGAAGCTCCAAGACCAAAGGAACCATCGCAAAATGTTGATGAGCAAAAGGGTACCCCAAAAGCTAAAGAAGAGAAAGCAGAAACAAAGGAAACAACAACAAATCCACCAGAAGAAGCTCCAAGACCAAAGGAATCGCCACAAAAGGCTGATGAGCAAAAGGGTGCTCAAGAGGGTACCCCAAAAGCTATAGAAGAGAAAGCAGAAACAAAGACTAATGATGTCTCCGATCAAAAGATACCACCACAAAAGGAGAATAATACAATAACCGAAAAGAGCAGAACAGAAGCTAGCATAGACAAAGTGGCGGAGAAAGTGAGGACTAATGGCTCGGCTGAGACAATAGACGCAGCAACATCTAATTCTCCTAAAACCAAAGATGCCAAGTTTATTGCTAGATGCAAAACCAGGCTTGTAGATTTCACTCTCTCTAAGGCTCTCTGTAATCAGGATGAAAATGAAGCCCTTGGAAATTCGACCACAGGTCTTAAGAAGTGGAAGAGATTGGTTGCATGTGTCATGCTAATCTTATTGGTCGTGGGACTTGGGCTTTATTGTAGAAATACATTTGGATCGTCTCAGGGAGAATTGGACCTCGAAGAGCTGTTATTATTTCCTTACTGA
Protein sequence

>Msa0860800

MDTKTQPEADRVYEDFEPYHEWDKYDGRFTVMLPGYRRDQMKVQVTSKPALRLIGERPTFQNRWRRFKLEFPIPSDYDTDSVTATFEGGKLSVKFAKLTNPKETTTNPPEEAPRPKEPSQKVDEQKGAPKAKEEKVATKETTTNPPEEAPRPKEPSQNVDEQKGTPKAKEEKAETKETTTNPPEEAPRPKESPQKADEQKGAQEGTPKAIEEKAETKTNDVSDQKIPPQKENNTITEKSRTEASIDKVAEKVRTNGSAETIDAATSNSPKTKDAKFIARCKTRLVDFTLSKALCNQDENEALGNSTTGLKKWKRLVACVMLILLVVGLGLYCRNTFGSSQGELDLEELLLFPY*