Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0864060 | Msa0864070 | 0.800915 | 1.151656e-48 | -8.615850e-47 |
| Msa0864060 | Msa1270240 | 0.802631 | 5.119491e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCGTTTGTTAAATCATTT+AGG | 0.281266 | 5_4:-64171094 | Msa0864060:CDS |
| CGAGCTGCTAGATTAGGTTT+TGG | 0.331839 | 5_4:-64171185 | Msa0864060:CDS |
| GCTGTTTGGGAACTAGTTAA+TGG | 0.356582 | 5_4:-64171748 | Msa0864060:CDS |
| CAAGATTGAGATGGCTGTTT+GGG | 0.360205 | 5_4:-64171761 | None:intergenic |
| CCACAGTGTTTACTAATAGA+TGG | 0.393695 | 5_4:-64171660 | Msa0864060:CDS |
| TCCTAGCTAGAAAGGTGATA+TGG | 0.410532 | 5_4:+64171716 | None:intergenic |
| TCACTGCGAGCTGCTAGATT+AGG | 0.430552 | 5_4:-64171191 | Msa0864060:CDS |
| TCAAGATTGAGATGGCTGTT+TGG | 0.443129 | 5_4:-64171762 | None:intergenic |
| CGGATAAGATGGAAGATAAG+AGG | 0.483166 | 5_4:+64171145 | None:intergenic |
| TAGCATAATAACGGATAAGA+TGG | 0.485732 | 5_4:+64171134 | None:intergenic |
| ACCATATCACCTTTCTAGCT+AGG | 0.492797 | 5_4:-64171717 | Msa0864060:CDS |
| TCTTATCCGTTATTATGCTA+TGG | 0.498677 | 5_4:-64171131 | Msa0864060:CDS |
| CTAGAAAGGTGATATGGTAA+AGG | 0.506004 | 5_4:+64171722 | None:intergenic |
| ATCAGCATTCCTAGCTAGAA+AGG | 0.535370 | 5_4:+64171708 | None:intergenic |
| AGCTTGCCATAGCATAATAA+CGG | 0.565019 | 5_4:+64171125 | None:intergenic |
| AAGATGGAAGATAAGAGGAT+CGG | 0.605242 | 5_4:+64171150 | None:intergenic |
| TGTTTACTAATAGATGGATG+AGG | 0.629998 | 5_4:-64171654 | Msa0864060:CDS |
| TACTAATAGATGGATGAGGA+AGG | 0.630665 | 5_4:-64171650 | Msa0864060:intron |
| ATGATTTAACAAACGAACGC+AGG | 0.657550 | 5_4:+64171099 | None:intergenic |
| CCATCTATTAGTAAACACTG+TGG | 0.792095 | 5_4:+64171660 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGCAAATAATATTTTTGTTA+GGG | + | chr5_4:64171453-64171472 | None:intergenic | 15.0% |
| !!! | AAGCAAATAATATTTTTGTT+AGG | + | chr5_4:64171454-64171473 | None:intergenic | 15.0% |
| !!! | GCAAATAATATTTTTGTTAG+GGG | + | chr5_4:64171452-64171471 | None:intergenic | 20.0% |
| ! | ATAGACTGAATTTACATAAC+TGG | + | chr5_4:64171503-64171522 | None:intergenic | 25.0% |
| ! | TGTACTCTAAATCATATAGA+AGG | + | chr5_4:64171552-64171571 | None:intergenic | 25.0% |
| !! | GGTTCTTGTTTACATAATTA+CGG | - | chr5_4:64171581-64171600 | Msa0864060:intron | 25.0% |
| !! | GTTCTTGTTTACATAATTAC+GGG | - | chr5_4:64171582-64171601 | Msa0864060:intron | 25.0% |
| !! | GTTCGTTTGTTAAATCATTT+AGG | - | chr5_4:64171739-64171758 | Msa0864060:CDS | 25.0% |
| TGTTTACTAATAGATGGATG+AGG | - | chr5_4:64171179-64171198 | Msa0864060:CDS | 30.0% | |
| GCAAAATAAGAAAGACTGAA+AGG | + | chr5_4:64171277-64171296 | None:intergenic | 30.0% | |
| !!! | AGTTTTTTGTCCAACGAAAA+AGG | - | chr5_4:64171386-64171405 | Msa0864060:intron | 30.0% |
| !!! | GTTTTTTGTCCAACGAAAAA+GGG | - | chr5_4:64171387-64171406 | Msa0864060:intron | 30.0% |
| !!! | TTTTTTGTCCAACGAAAAAG+GGG | - | chr5_4:64171388-64171407 | Msa0864060:intron | 30.0% |
| ACTGAATTTACATAACTGGA+AGG | + | chr5_4:64171499-64171518 | None:intergenic | 30.0% | |
| ! | ATTTAGAGTACATGCTCATT+TGG | - | chr5_4:64171560-64171579 | Msa0864060:intron | 30.0% |
| !!! | TTTGTTTGCTTGTTTTGTTC+AGG | - | chr5_4:64171608-64171627 | Msa0864060:intron | 30.0% |
| !! | TAGCATAATAACGGATAAGA+TGG | + | chr5_4:64171702-64171721 | None:intergenic | 30.0% |
| TCTTATCCGTTATTATGCTA+TGG | - | chr5_4:64171702-64171721 | Msa0864060:CDS | 30.0% | |
| CTAGAAAGGTGATATGGTAA+AGG | + | chr5_4:64171114-64171133 | None:intergenic | 35.0% | |
| CCATCTATTAGTAAACACTG+TGG | + | chr5_4:64171176-64171195 | None:intergenic | 35.0% | |
| CCACAGTGTTTACTAATAGA+TGG | - | chr5_4:64171173-64171192 | Msa0864060:CDS | 35.0% | |
| TACTAATAGATGGATGAGGA+AGG | - | chr5_4:64171183-64171202 | Msa0864060:CDS | 35.0% | |
| GAATCAAAAGTAGCGATGTT+TGG | - | chr5_4:64171218-64171237 | Msa0864060:intron | 35.0% | |
| TCAGATGCAGAGAAACATAT+TGG | + | chr5_4:64171341-64171360 | None:intergenic | 35.0% | |
| AAGATGGAAGATAAGAGGAT+CGG | + | chr5_4:64171686-64171705 | None:intergenic | 35.0% | |
| AGCTTGCCATAGCATAATAA+CGG | + | chr5_4:64171711-64171730 | None:intergenic | 35.0% | |
| ATGATTTAACAAACGAACGC+AGG | + | chr5_4:64171737-64171756 | None:intergenic | 35.0% | |
| GCTGTTTGGGAACTAGTTAA+TGG | - | chr5_4:64171085-64171104 | Msa0864060:CDS | 40.0% | |
| !! | ACCATATCACCTTTCTAGCT+AGG | - | chr5_4:64171116-64171135 | Msa0864060:CDS | 40.0% |
| TCCTAGCTAGAAAGGTGATA+TGG | + | chr5_4:64171120-64171139 | None:intergenic | 40.0% | |
| ATCAGCATTCCTAGCTAGAA+AGG | + | chr5_4:64171128-64171147 | None:intergenic | 40.0% | |
| ATAGATGGATGAGGAAGGTT+CGG | - | chr5_4:64171188-64171207 | Msa0864060:CDS | 40.0% | |
| GCTCTCAAGAATCAAGGTAA+AGG | + | chr5_4:64171530-64171549 | None:intergenic | 40.0% | |
| TAGAAGGCTCTCAAGAATCA+AGG | + | chr5_4:64171536-64171555 | None:intergenic | 40.0% | |
| !!! | CTTGTTTTGTTCAGGTACGT+TGG | - | chr5_4:64171616-64171635 | Msa0864060:intron | 40.0% |
| CGGATAAGATGGAAGATAAG+AGG | + | chr5_4:64171691-64171710 | None:intergenic | 40.0% | |
| GTCCAACGAAAAAGGGGATA+AGG | - | chr5_4:64171394-64171413 | Msa0864060:intron | 45.0% | |
| !! | AGCCTTATCCCCTTTTTCGT+TGG | + | chr5_4:64171399-64171418 | None:intergenic | 45.0% |
| ! | CGAGCTGCTAGATTAGGTTT+TGG | - | chr5_4:64171648-64171667 | Msa0864060:intron | 45.0% |
| ATGAGGAAGGTTCGGAGAGT+CGG | - | chr5_4:64171196-64171215 | Msa0864060:CDS | 50.0% | |
| TCACTGCGAGCTGCTAGATT+AGG | - | chr5_4:64171642-64171661 | Msa0864060:intron | 50.0% | |
| AAGGCTAACCTCCCATGCAG+TGG | - | chr5_4:64171413-64171432 | Msa0864060:intron | 55.0% | |
| AGGCTAACCTCCCATGCAGT+GGG | - | chr5_4:64171414-64171433 | Msa0864060:intron | 55.0% | |
| AGCTAACACCCCCACTGCAT+GGG | + | chr5_4:64171427-64171446 | None:intergenic | 55.0% | |
| TAGCTAACACCCCCACTGCA+TGG | + | chr5_4:64171428-64171447 | None:intergenic | 55.0% | |
| GGCTAACCTCCCATGCAGTG+GGG | - | chr5_4:64171415-64171434 | Msa0864060:intron | 60.0% | |
| GCTAACCTCCCATGCAGTGG+GGG | - | chr5_4:64171416-64171435 | Msa0864060:intron | 60.0% | |
| TAACACCCCCACTGCATGGG+AGG | + | chr5_4:64171424-64171443 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 64171082 | 64171773 | 64171082 | ID=Msa0864060;Name=Msa0864060 |
| chr5_4 | mRNA | 64171082 | 64171773 | 64171082 | ID=Msa0864060-mRNA-1;Parent=Msa0864060;Name=Msa0864060-mRNA-1;_AED=0.64;_eAED=0.65;_QI=0|0|0|0.5|1|1|2|0|88 |
| chr5_4 | exon | 64171651 | 64171773 | 64171651 | ID=Msa0864060-mRNA-1:exon:20222;Parent=Msa0864060-mRNA-1 |
| chr5_4 | exon | 64171082 | 64171225 | 64171082 | ID=Msa0864060-mRNA-1:exon:20221;Parent=Msa0864060-mRNA-1 |
| chr5_4 | CDS | 64171651 | 64171773 | 64171651 | ID=Msa0864060-mRNA-1:cds;Parent=Msa0864060-mRNA-1 |
| chr5_4 | CDS | 64171082 | 64171225 | 64171082 | ID=Msa0864060-mRNA-1:cds;Parent=Msa0864060-mRNA-1 |
| Gene Sequence |
| Protein sequence |
