AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1270240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1270240 MtrunA17_Chr8g0355041 75.238 105 21 2 8 107 483 587 1.06e-43 150
Msa1270240 MtrunA17_Chr5g0415181 55.000 100 25 2 8 107 400 479 5.25e-24 95.1
Msa1270240 MtrunA17_Chr8g0355051 54.762 84 32 3 29 107 1 83 5.04e-21 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1270240 AT1G60560.1 60.000 105 37 2 8 107 481 585 1.28e-34 125

Find 19 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCACAGTGGTAGGCTTCT+TGG 0.245815 8_3:+3696397 None:intergenic
TTCATTCTGTGATAGGTTCT+TGG 0.310754 8_3:-3696079 Msa1270240:CDS
GTCTTTCTTGCGCACAACTT+TGG 0.394069 8_3:+3696143 None:intergenic
TTGAGTTGCATTCAAGCTAC+TGG 0.449714 8_3:-3696298 Msa1270240:CDS
AACACATATAGTGTGGAATC+GGG 0.456647 8_3:-3696112 Msa1270240:CDS
TAACACATATAGTGTGGAAT+CGG 0.468672 8_3:-3696113 Msa1270240:CDS
GCTTTCAGTTTCACAGTGGT+AGG 0.491986 8_3:+3696389 None:intergenic
TCTTTCCAGAATGTGAAAGA+AGG 0.512792 8_3:-3696248 Msa1270240:intron
TCTAAGGTGACAGCAGAGTC+GGG 0.520386 8_3:+3696186 None:intergenic
GTCTAAGGTGACAGCAGAGT+CGG 0.532861 8_3:+3696185 None:intergenic
TGCGCACAACTTTGGCAAAG+AGG 0.548038 8_3:+3696151 None:intergenic
GTTATATTGCTTCTACGTCA+TGG 0.555768 8_3:-3696226 Msa1270240:CDS
GCAACTCAATTGTTACCTTG+TGG 0.556438 8_3:+3696312 None:intergenic
AGTAGATTGTTTAGTCCACA+AGG 0.612089 8_3:-3696327 Msa1270240:CDS
ATAGGTTCTTGGTCATTGCA+TGG 0.631361 8_3:-3696068 Msa1270240:CDS
GCAGCTTAACACATATAGTG+TGG 0.647606 8_3:-3696119 Msa1270240:CDS
GCACAACTTTGGCAAAGAGG+CGG 0.647684 8_3:+3696154 None:intergenic
ACACATATAGTGTGGAATCG+GGG 0.674995 8_3:-3696111 Msa1270240:CDS
TTTGGCTTTCAGTTTCACAG+TGG 0.743653 8_3:+3696385 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAAAGTATTAGTCTTTTT+TGG - chr8_3:3696196-3696215 Msa1270240:CDS 15.0%
!!! TTAAAAGTATTAGTCTTTTT+TGG - chr8_3:3696196-3696215 Msa1270240:CDS 15.0%
!!! AAAAATTTGATAACGTTACA+AGG - chr8_3:3696309-3696328 Msa1270240:CDS 20.0%
!! AACATTTCAGATGTAATATA+TGG + chr8_3:3696364-3696383 None:intergenic 20.0%
!!! AAAAATGACAAATTTTCTAC+TGG - chr8_3:3696493-3696512 Msa1270240:intron 20.0%
!!! AAAATGACAAATTTTCTACT+GGG - chr8_3:3696494-3696513 Msa1270240:intron 20.0%
!! ATGTGAATCATCAAAAAATT+TGG + chr8_3:3696553-3696572 None:intergenic 20.0%
!!! AAAAATTTGATAACGTTACA+AGG - chr8_3:3696309-3696328 Msa1270240:CDS 20.0%
!! AACATTTCAGATGTAATATA+TGG + chr8_3:3696364-3696383 None:intergenic 20.0%
!!! AAAAATGACAAATTTTCTAC+TGG - chr8_3:3696493-3696512 Msa1270240:intron 20.0%
!!! AAAATGACAAATTTTCTACT+GGG - chr8_3:3696494-3696513 Msa1270240:intron 20.0%
!! ATGTGAATCATCAAAAAATT+TGG + chr8_3:3696553-3696572 None:intergenic 20.0%
!! TCATTTTGCTTCAAATGTTT+CGG - chr8_3:3696388-3696407 Msa1270240:CDS 25.0%
!!! TTTTTTGATGATTCACATCT+CGG - chr8_3:3696555-3696574 Msa1270240:intron 25.0%
!! TCATTTTGCTTCAAATGTTT+CGG - chr8_3:3696388-3696407 Msa1270240:CDS 25.0%
!!! TTTTTTGATGATTCACATCT+CGG - chr8_3:3696555-3696574 Msa1270240:intron 25.0%
CAGAATATGTGGTAACAATA+CGG + chr8_3:3696335-3696354 None:intergenic 30.0%
! CATTTTGCTTCAAATGTTTC+GGG - chr8_3:3696389-3696408 Msa1270240:CDS 30.0%
TATAACCTTCTTTCACATTC+TGG + chr8_3:3696677-3696696 None:intergenic 30.0%
TAACACATATAGTGTGGAAT+CGG - chr8_3:3696804-3696823 Msa1270240:intron 30.0%
! CTGAATTTTCATTCTGTGAT+AGG - chr8_3:3696831-3696850 Msa1270240:intron 30.0%
CAGAATATGTGGTAACAATA+CGG + chr8_3:3696335-3696354 None:intergenic 30.0%
! CATTTTGCTTCAAATGTTTC+GGG - chr8_3:3696389-3696408 Msa1270240:CDS 30.0%
TATAACCTTCTTTCACATTC+TGG + chr8_3:3696677-3696696 None:intergenic 30.0%
TAACACATATAGTGTGGAAT+CGG - chr8_3:3696804-3696823 Msa1270240:intron 30.0%
! CTGAATTTTCATTCTGTGAT+AGG - chr8_3:3696831-3696850 Msa1270240:intron 30.0%
CGTATTGTTACCACATATTC+TGG - chr8_3:3696333-3696352 Msa1270240:CDS 35.0%
! TCGTTGATTATGTTAGCTTG+TGG - chr8_3:3696425-3696444 Msa1270240:intron 35.0%
AGTAGATTGTTTAGTCCACA+AGG - chr8_3:3696590-3696609 Msa1270240:intron 35.0%
TCTTTCCAGAATGTGAAAGA+AGG - chr8_3:3696669-3696688 Msa1270240:intron 35.0%
GTTATATTGCTTCTACGTCA+TGG - chr8_3:3696691-3696710 Msa1270240:intron 35.0%
AACACATATAGTGTGGAATC+GGG - chr8_3:3696805-3696824 Msa1270240:intron 35.0%
TTCATTCTGTGATAGGTTCT+TGG - chr8_3:3696838-3696857 Msa1270240:intron 35.0%
CGTATTGTTACCACATATTC+TGG - chr8_3:3696333-3696352 Msa1270240:CDS 35.0%
! TCGTTGATTATGTTAGCTTG+TGG - chr8_3:3696425-3696444 Msa1270240:intron 35.0%
AGTAGATTGTTTAGTCCACA+AGG - chr8_3:3696590-3696609 Msa1270240:intron 35.0%
TCTTTCCAGAATGTGAAAGA+AGG - chr8_3:3696669-3696688 Msa1270240:intron 35.0%
GTTATATTGCTTCTACGTCA+TGG - chr8_3:3696691-3696710 Msa1270240:intron 35.0%
AACACATATAGTGTGGAATC+GGG - chr8_3:3696805-3696824 Msa1270240:intron 35.0%
TTCATTCTGTGATAGGTTCT+TGG - chr8_3:3696838-3696857 Msa1270240:intron 35.0%
TATGGTGAAGCCAGAATATG+TGG + chr8_3:3696346-3696365 None:intergenic 40.0%
!! TTTGGCTTTCAGTTTCACAG+TGG + chr8_3:3696535-3696554 None:intergenic 40.0%
GCAACTCAATTGTTACCTTG+TGG + chr8_3:3696608-3696627 None:intergenic 40.0%
! TTGAGTTGCATTCAAGCTAC+TGG - chr8_3:3696619-3696638 Msa1270240:intron 40.0%
GCAGCTTAACACATATAGTG+TGG - chr8_3:3696798-3696817 Msa1270240:intron 40.0%
ACACATATAGTGTGGAATCG+GGG - chr8_3:3696806-3696825 Msa1270240:intron 40.0%
ATAGGTTCTTGGTCATTGCA+TGG - chr8_3:3696849-3696868 Msa1270240:intron 40.0%
TATGGTGAAGCCAGAATATG+TGG + chr8_3:3696346-3696365 None:intergenic 40.0%
!! TTTGGCTTTCAGTTTCACAG+TGG + chr8_3:3696535-3696554 None:intergenic 40.0%
GCAACTCAATTGTTACCTTG+TGG + chr8_3:3696608-3696627 None:intergenic 40.0%
! TTGAGTTGCATTCAAGCTAC+TGG - chr8_3:3696619-3696638 Msa1270240:intron 40.0%
GCAGCTTAACACATATAGTG+TGG - chr8_3:3696798-3696817 Msa1270240:intron 40.0%
ACACATATAGTGTGGAATCG+GGG - chr8_3:3696806-3696825 Msa1270240:intron 40.0%
ATAGGTTCTTGGTCATTGCA+TGG - chr8_3:3696849-3696868 Msa1270240:intron 40.0%
TTTCACAGTGGTAGGCTTCT+TGG + chr8_3:3696523-3696542 None:intergenic 45.0%
GCTTTCAGTTTCACAGTGGT+AGG + chr8_3:3696531-3696550 None:intergenic 45.0%
! GAGGCGGTTTTTATCGTCTA+AGG + chr8_3:3696750-3696769 None:intergenic 45.0%
GTCTTTCTTGCGCACAACTT+TGG + chr8_3:3696777-3696796 None:intergenic 45.0%
TTTCACAGTGGTAGGCTTCT+TGG + chr8_3:3696523-3696542 None:intergenic 45.0%
GCTTTCAGTTTCACAGTGGT+AGG + chr8_3:3696531-3696550 None:intergenic 45.0%
! GAGGCGGTTTTTATCGTCTA+AGG + chr8_3:3696750-3696769 None:intergenic 45.0%
GTCTTTCTTGCGCACAACTT+TGG + chr8_3:3696777-3696796 None:intergenic 45.0%
AGCAGAGGAAAGGAAGGGTA+AGG + chr8_3:3696454-3696473 None:intergenic 50.0%
TGCACAGCAGAGGAAAGGAA+GGG + chr8_3:3696459-3696478 None:intergenic 50.0%
GTTTCTGCACAGCAGAGGAA+AGG + chr8_3:3696464-3696483 None:intergenic 50.0%
! TCTAAGGTGACAGCAGAGTC+GGG + chr8_3:3696734-3696753 None:intergenic 50.0%
! GTCTAAGGTGACAGCAGAGT+CGG + chr8_3:3696735-3696754 None:intergenic 50.0%
GCACAACTTTGGCAAAGAGG+CGG + chr8_3:3696766-3696785 None:intergenic 50.0%
TGCGCACAACTTTGGCAAAG+AGG + chr8_3:3696769-3696788 None:intergenic 50.0%
AGCAGAGGAAAGGAAGGGTA+AGG + chr8_3:3696454-3696473 None:intergenic 50.0%
TGCACAGCAGAGGAAAGGAA+GGG + chr8_3:3696459-3696478 None:intergenic 50.0%
GTTTCTGCACAGCAGAGGAA+AGG + chr8_3:3696464-3696483 None:intergenic 50.0%
! TCTAAGGTGACAGCAGAGTC+GGG + chr8_3:3696734-3696753 None:intergenic 50.0%
! GTCTAAGGTGACAGCAGAGT+CGG + chr8_3:3696735-3696754 None:intergenic 50.0%
GCACAACTTTGGCAAAGAGG+CGG + chr8_3:3696766-3696785 None:intergenic 50.0%
TGCGCACAACTTTGGCAAAG+AGG + chr8_3:3696769-3696788 None:intergenic 50.0%
CTGCACAGCAGAGGAAAGGA+AGG + chr8_3:3696460-3696479 None:intergenic 55.0%
GTCACGTTTCTGCACAGCAG+AGG + chr8_3:3696469-3696488 None:intergenic 55.0%
CTGCACAGCAGAGGAAAGGA+AGG + chr8_3:3696460-3696479 None:intergenic 55.0%
GTCACGTTTCTGCACAGCAG+AGG + chr8_3:3696469-3696488 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 3696065 3696874 3696065 ID=Msa1270240;Name=Msa1270240
chr8_3 mRNA 3696065 3696874 3696065 ID=Msa1270240-mRNA-1;Parent=Msa1270240;Name=Msa1270240-mRNA-1;_AED=0.49;_eAED=0.55;_QI=0|0|0|0.66|0|0|3|0|114
chr8_3 exon 3696871 3696874 3696871 ID=Msa1270240-mRNA-1:exon:1202;Parent=Msa1270240-mRNA-1
chr8_3 exon 3696276 3696419 3696276 ID=Msa1270240-mRNA-1:exon:1201;Parent=Msa1270240-mRNA-1
chr8_3 exon 3696065 3696261 3696065 ID=Msa1270240-mRNA-1:exon:1200;Parent=Msa1270240-mRNA-1
chr8_3 CDS 3696871 3696874 3696871 ID=Msa1270240-mRNA-1:cds;Parent=Msa1270240-mRNA-1
chr8_3 CDS 3696276 3696419 3696276 ID=Msa1270240-mRNA-1:cds;Parent=Msa1270240-mRNA-1
chr8_3 CDS 3696065 3696261 3696065 ID=Msa1270240-mRNA-1:cds;Parent=Msa1270240-mRNA-1
Gene Sequence

>Msa1270240

ATGGCCAAGAAGCCTACCACTGTGAAACTGAAAGCCAAATTTTTTGATGATTCACATCTCGGAGTGCTGCAAAGAGTAGATTGTTTAGTCCACAAGGTAACAATTGAGTTGCATTCAAGCTACTGGCTTGATCGTTTCGCTGATGAAAAATGTGAAAGAAGGTTATATTGCTTCTACGTCATGGCACAAGCACTTGAAATTCCCGACTCTGCTGTCACCTTAGACGATAAAAACCGCCTCTTTGCCAAAGTTGTGCGCAAGAAAGACAGCAGCTTAACACATATAGTGTGGAATCGGGGATCTGAATTTTCATTCTGTGATAGGTTCTTGGTCATTGCATGGTAA

Protein sequence

>Msa1270240

MAKKPTTVKLKAKFFDDSHLGVLQRVDCLVHKVTIELHSSYWLDRFADEKCERRLYCFYVMAQALEIPDSAVTLDDKNRLFAKVVRKKDSSLTHIVWNRGSEFSFCDRFLVIAW*