AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0871900


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0871900 MtrunA17_Chr6g0453221 94.118 85 1 1 1 85 1 81 1.27e-53 161
Msa0871900 MtrunA17_Chr3g0092371 68.675 83 23 2 1 81 1 82 2.18e-36 117
Msa0871900 MtrunA17_Chr1g0188441 58.209 67 28 0 15 81 3 69 2.88e-23 87.8
Msa0871900 MtrunA17_Chr4g0060731 65.333 75 19 3 1 71 1 72 5.31e-23 84.0
Msa0871900 MtrunA17_Chr3g0133181 60.000 55 22 0 18 72 77 131 1.03e-20 82.8
Msa0871900 MtrunA17_Chr2g0315941 56.923 65 26 1 21 83 68 132 3.25e-20 81.3
Msa0871900 MtrunA17_Chr8g0367691 56.061 66 27 1 20 83 68 133 4.70e-20 80.9
Msa0871900 MtrunA17_Chr8g0361671 67.273 55 16 1 18 70 61 115 1.92e-19 80.5
Msa0871900 MtrunA17_Chr4g0076821 63.158 57 21 0 20 76 112 168 1.94e-19 80.5
Msa0871900 MtrunA17_Chr7g0217231 59.322 59 22 1 16 72 83 141 2.48e-19 79.7
Msa0871900 MtrunA17_Chr5g0412501 54.098 61 28 0 20 80 77 137 6.21e-19 77.8
Msa0871900 MtrunA17_Chr5g0427051 56.667 60 26 0 19 78 56 115 1.00e-18 77.4
Msa0871900 MtrunA17_Chr5g0404271 59.615 52 21 0 21 72 99 150 3.33e-18 76.3
Msa0871900 MtrunA17_Chr6g0453231 61.111 54 19 1 21 72 91 144 4.83e-17 73.6
Msa0871900 MtrunA17_Chr6g0479901 51.667 60 28 1 21 79 19 78 1.33e-15 67.4
Msa0871900 MtrunA17_Chr8g0357721 50.000 38 19 0 30 67 14 51 1.30e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0871900 AT3G28917.1 59.596 99 25 3 1 84 1 99 8.03e-32 107
Msa0871900 AT1G18835.1 60.000 90 26 3 1 83 1 87 3.92e-30 102
Msa0871900 AT1G74660.1 50.495 101 31 2 1 82 2 102 6.60e-28 97.4
Msa0871900 AT1G74660.2 50.562 89 27 1 1 72 2 90 4.14e-26 92.4
Msa0871900 AT4G24660.1 66.071 56 19 0 19 74 47 102 3.65e-23 88.6
Msa0871900 AT4G24660.2 66.071 56 19 0 19 74 47 102 4.57e-23 88.6
Msa0871900 AT2G02540.2 55.224 67 30 0 6 72 72 138 2.86e-22 87.8
Msa0871900 AT2G02540.1 55.224 67 30 0 6 72 72 138 2.86e-22 87.8
Msa0871900 AT1G14440.1 55.072 69 29 1 15 81 84 152 9.02e-22 86.3
Msa0871900 AT1G14440.2 55.072 69 29 1 15 81 84 152 9.02e-22 86.3
Msa0871900 AT5G15210.1 60.938 64 23 1 17 78 52 115 1.01e-20 83.2
Msa0871900 AT3G50890.1 54.412 68 28 1 7 71 43 110 3.42e-20 81.3
Msa0871900 AT1G75240.1 59.677 62 25 0 16 77 71 132 5.38e-20 81.6
Msa0871900 AT2G18350.1 58.209 67 25 1 19 82 80 146 1.13e-19 80.1
Msa0871900 AT5G65410.1 60.000 55 22 0 17 71 71 125 2.13e-19 79.7
Msa0871900 AT1G69600.1 64.815 54 17 1 19 70 29 82 2.15e-18 76.3
Msa0871900 AT3G28920.1 59.375 64 24 1 19 80 50 113 4.11e-16 71.2
Msa0871900 AT5G60480.1 59.259 54 20 1 18 69 34 87 4.82e-16 70.1
Msa0871900 AT5G42780.1 60.784 51 18 1 21 71 64 112 1.96e-15 68.6
Msa0871900 AT5G39760.1 49.333 75 36 1 6 78 41 115 4.08e-15 68.6
Msa0871900 AT1G14687.1 50.769 65 23 4 21 85 7 62 6.24e-15 65.9

Find 11 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGAAGTGTTGTGAAATA+TGG 0.276912 6_1:+7153509 Msa0871900:CDS
GTTGGAGGTTATGCTGTTGA+TGG 0.403038 6_1:+7153560 Msa0871900:CDS
TTTGTACCTTGTTCACTACT+TGG 0.415225 6_1:-7153599 None:intergenic
TATTTCACAACACTTCTTTG+TGG 0.421230 6_1:-7153506 None:intergenic
AGTAGTGAACAAGGTACAAA+TGG 0.484103 6_1:+7153602 Msa0871900:CDS
AGTTCTTCACCTCACTCAAA+TGG 0.507969 6_1:+7153695 Msa0871900:CDS
TCTCTCACTTGTGCTGCTTG+TGG 0.516521 6_1:+7153626 Msa0871900:CDS
AAGAATCATGCAGCTAATGT+TGG 0.517780 6_1:+7153542 Msa0871900:CDS
AATCATGCAGCTAATGTTGG+AGG 0.576991 6_1:+7153545 Msa0871900:CDS
TTTCAAGTACCATTTGAGTG+AGG 0.642570 6_1:-7153704 None:intergenic
TTCATGCCAAGTAGTGAACA+AGG 0.658987 6_1:+7153593 Msa0871900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TATTTCACAACACTTCTTTG+TGG - chr6_1:7153509-7153528 None:intergenic 30.0%
!! CAAAGAAGTGTTGTGAAATA+TGG + chr6_1:7153509-7153528 Msa0871900:CDS 30.0%
AAGAATCATGCAGCTAATGT+TGG + chr6_1:7153542-7153561 Msa0871900:CDS 35.0%
TTTGTACCTTGTTCACTACT+TGG - chr6_1:7153602-7153621 None:intergenic 35.0%
AGTAGTGAACAAGGTACAAA+TGG + chr6_1:7153602-7153621 Msa0871900:CDS 35.0%
AATCATGCAGCTAATGTTGG+AGG + chr6_1:7153545-7153564 Msa0871900:CDS 40.0%
TTCATGCCAAGTAGTGAACA+AGG + chr6_1:7153593-7153612 Msa0871900:CDS 40.0%
AGTTCTTCACCTCACTCAAA+TGG + chr6_1:7153695-7153714 Msa0871900:CDS 40.0%
GTTGGAGGTTATGCTGTTGA+TGG + chr6_1:7153560-7153579 Msa0871900:CDS 45.0%
!! TCTCTCACTTGTGCTGCTTG+TGG + chr6_1:7153626-7153645 Msa0871900:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 7153467 7153724 7153467 ID=Msa0871900;Name=Msa0871900
chr6_1 mRNA 7153467 7153724 7153467 ID=Msa0871900-mRNA-1;Parent=Msa0871900;Name=Msa0871900-mRNA-1;_AED=0.27;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|85
chr6_1 exon 7153467 7153724 7153467 ID=Msa0871900-mRNA-1:exon:2162;Parent=Msa0871900-mRNA-1
chr6_1 CDS 7153467 7153724 7153467 ID=Msa0871900-mRNA-1:cds;Parent=Msa0871900-mRNA-1
Gene Sequence

>Msa0871900

ATGAGGAAGAGACAAGTAGTTGTGAGAAGATCAGAAGATCCACAAAGAAGTGTTGTGAAATATGGAGAGTGTCAGAAGAATCATGCAGCTAATGTTGGAGGTTATGCTGTTGATGGTTGTAGAGAGTTCATGCCAAGTAGTGAACAAGGTACAAATGGTTCTCTCACTTGTGCTGCTTGTGGTTGTCACAGAAACTTTCACAAAAGAGAAGTTGAAGTTGTTTCTGAGAGTTCTTCACCTCACTCAAATGGTACTTGA

Protein sequence

>Msa0871900

MRKRQVVVRRSEDPQRSVVKYGECQKNHAANVGGYAVDGCREFMPSSEQGTNGSLTCAACGCHRNFHKREVEVVSESSSPHSNGT*