Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0322830 | Msa0876670 | 0.804598 | 2.000617e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0876670 | MtrunA17_Chr3g0089261 | 67.241 | 58 | 15 | 2 | 45 | 98 | 139 | 196 | 1.99e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACAGCCCTTGTACTAAT+AGG | 0.207882 | 6_1:+15051226 | None:intergenic |
| CTTTCAATAGCACTTGTAAT+AGG | 0.214722 | 6_1:+15051067 | None:intergenic |
| TTTCAATAGCACTTGTAATA+GGG | 0.290537 | 6_1:+15051068 | None:intergenic |
| AGATTATTTCGAAGGATATC+AGG | 0.292739 | 6_1:-15051176 | Msa0876670:CDS |
| CAACAGCCCTTGTACTAATA+GGG | 0.295589 | 6_1:+15051227 | None:intergenic |
| GGAAGAAGATGAAGAGAAAA+TGG | 0.338955 | 6_1:-15050972 | Msa0876670:CDS |
| ATATCAATGGAACGATAGTT+TGG | 0.382670 | 6_1:-15050993 | Msa0876670:CDS |
| ATATCAGGGGAACAATGATT+TGG | 0.392944 | 6_1:-15051161 | Msa0876670:CDS |
| GGAAGAAGATGAAGAGGAAA+TGG | 0.404037 | 6_1:-15051140 | Msa0876670:CDS |
| GTATATAATCGGTTCGAGTC+CGG | 0.423189 | 6_1:-15050938 | Msa0876670:CDS |
| GTTAATGATGAAGATGATTA+CGG | 0.442607 | 6_1:-15051019 | Msa0876670:CDS |
| GATTATGAAGATTATTTCGA+AGG | 0.485832 | 6_1:-15051184 | Msa0876670:CDS |
| AGAGGAAATTGAGGTTGAAG+AGG | 0.503049 | 6_1:-15051110 | Msa0876670:CDS |
| TTCAATAGCACTTGTAATAG+GGG | 0.508231 | 6_1:+15051069 | None:intergenic |
| AGAGGAAATGGAGGTTGAAG+AGG | 0.518218 | 6_1:-15051128 | Msa0876670:CDS |
| GGAGGTTGAAGAGGAAATTG+AGG | 0.526203 | 6_1:-15051119 | Msa0876670:CDS |
| GATTATTTCGAAGGATATCA+GGG | 0.534381 | 6_1:-15051175 | Msa0876670:CDS |
| AGGTTGAAGAAGTATATAAT+CGG | 0.535082 | 6_1:-15050949 | Msa0876670:CDS |
| AGAAGATGAAGAGGAAATGG+AGG | 0.547063 | 6_1:-15051137 | Msa0876670:CDS |
| AACAGCCCTTGTACTAATAG+GGG | 0.547908 | 6_1:+15051228 | None:intergenic |
| ATGATTACGGAGGATATCAA+TGG | 0.549796 | 6_1:-15051006 | Msa0876670:CDS |
| ATGTTACTATGCAGTGTGGC+GGG | 0.555419 | 6_1:-15051274 | Msa0876670:intron |
| TGATTTGGAAGAAGATGAAG+AGG | 0.561798 | 6_1:-15051146 | Msa0876670:CDS |
| TCAATAAATGACTTAGCCGC+CGG | 0.579194 | 6_1:+15050919 | None:intergenic |
| AGAAGATGAAGAGAAAATGG+AGG | 0.594524 | 6_1:-15050969 | Msa0876670:CDS |
| TATGTTACTATGCAGTGTGG+CGG | 0.630562 | 6_1:-15051275 | Msa0876670:intron |
| GGGTGGCAGTAGTAATGTGT+TGG | 0.652249 | 6_1:-15051254 | Msa0876670:CDS |
| GAGGTTGAAGAGGAAGACAA+TGG | 0.654903 | 6_1:-15051100 | Msa0876670:CDS |
| AATGATGAAGATGATTACGG+AGG | 0.682302 | 6_1:-15051016 | Msa0876670:CDS |
| ACAGCCCTTGTACTAATAGG+GGG | 0.685664 | 6_1:+15051229 | None:intergenic |
| ATTATTTCGAAGGATATCAG+GGG | 0.700321 | 6_1:-15051174 | Msa0876670:CDS |
| TTACTATGCAGTGTGGCGGG+TGG | 0.711237 | 6_1:-15051271 | Msa0876670:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAACATAAAACATATTTA+TGG | + | chr6_1:15051156-15051175 | None:intergenic | 15.0% |
| !!! | ATTCAAATAACCTTTTTGTT+TGG | - | chr6_1:15050986-15051005 | Msa0876670:CDS | 20.0% |
| !! | AACAATGTTTATATGCTATA+AGG | - | chr6_1:15051018-15051037 | Msa0876670:CDS | 20.0% |
| !!! | CTTTTTTTAGTGTAAAGTTT+AGG | - | chr6_1:15051050-15051069 | Msa0876670:CDS | 20.0% |
| !!! | TAACCTTTTTGTTTGGATAT+CGG | - | chr6_1:15050993-15051012 | Msa0876670:CDS | 25.0% |
| ! | TTATATGCTATAAGGATACT+CGG | - | chr6_1:15051026-15051045 | Msa0876670:CDS | 25.0% |
| ! | GATTATGAAGATTATTTCGA+AGG | - | chr6_1:15051258-15051277 | Msa0876670:CDS | 25.0% |
| ! | TTTCAATAGCACTTGTAATA+GGG | + | chr6_1:15051377-15051396 | None:intergenic | 25.0% |
| ! | GTTAATGATGAAGATGATTA+CGG | - | chr6_1:15051423-15051442 | Msa0876670:intron | 25.0% |
| !! | AGGTTGAAGAAGTATATAAT+CGG | - | chr6_1:15051493-15051512 | Msa0876670:intron | 25.0% |
| TTACCGATATCCAAACAAAA+AGG | + | chr6_1:15050999-15051018 | None:intergenic | 30.0% | |
| ! | TTTTATGTTACTATGCAGTG+TGG | - | chr6_1:15051164-15051183 | Msa0876670:CDS | 30.0% |
| AGATTATTTCGAAGGATATC+AGG | - | chr6_1:15051266-15051285 | Msa0876670:intron | 30.0% | |
| GATTATTTCGAAGGATATCA+GGG | - | chr6_1:15051267-15051286 | Msa0876670:intron | 30.0% | |
| ATTATTTCGAAGGATATCAG+GGG | - | chr6_1:15051268-15051287 | Msa0876670:intron | 30.0% | |
| TTCAATAGCACTTGTAATAG+GGG | + | chr6_1:15051376-15051395 | None:intergenic | 30.0% | |
| CTTTCAATAGCACTTGTAAT+AGG | + | chr6_1:15051378-15051397 | None:intergenic | 30.0% | |
| ATATCAATGGAACGATAGTT+TGG | - | chr6_1:15051449-15051468 | Msa0876670:intron | 30.0% | |
| ATATCAGGGGAACAATGATT+TGG | - | chr6_1:15051281-15051300 | Msa0876670:intron | 35.0% | |
| ! | TGATTTGGAAGAAGATGAAG+AGG | - | chr6_1:15051296-15051315 | Msa0876670:intron | 35.0% |
| AATGATGAAGATGATTACGG+AGG | - | chr6_1:15051426-15051445 | Msa0876670:intron | 35.0% | |
| ATGATTACGGAGGATATCAA+TGG | - | chr6_1:15051436-15051455 | Msa0876670:intron | 35.0% | |
| GGAAGAAGATGAAGAGAAAA+TGG | - | chr6_1:15051470-15051489 | Msa0876670:intron | 35.0% | |
| AGAAGATGAAGAGAAAATGG+AGG | - | chr6_1:15051473-15051492 | Msa0876670:intron | 35.0% | |
| ATACACACATAGAAGCTGAG+AGG | + | chr6_1:15050952-15050971 | None:intergenic | 40.0% | |
| TATGTTACTATGCAGTGTGG+CGG | - | chr6_1:15051167-15051186 | Msa0876670:CDS | 40.0% | |
| AACAGCCCTTGTACTAATAG+GGG | + | chr6_1:15051217-15051236 | None:intergenic | 40.0% | |
| CAACAGCCCTTGTACTAATA+GGG | + | chr6_1:15051218-15051237 | None:intergenic | 40.0% | |
| TCAACAGCCCTTGTACTAAT+AGG | + | chr6_1:15051219-15051238 | None:intergenic | 40.0% | |
| GGAAGAAGATGAAGAGGAAA+TGG | - | chr6_1:15051302-15051321 | Msa0876670:intron | 40.0% | |
| AGAAGATGAAGAGGAAATGG+AGG | - | chr6_1:15051305-15051324 | Msa0876670:intron | 40.0% | |
| AGAGGAAATTGAGGTTGAAG+AGG | - | chr6_1:15051332-15051351 | Msa0876670:intron | 40.0% | |
| !! | GTATATAATCGGTTCGAGTC+CGG | - | chr6_1:15051504-15051523 | Msa0876670:intron | 40.0% |
| ATGTTACTATGCAGTGTGGC+GGG | - | chr6_1:15051168-15051187 | Msa0876670:CDS | 45.0% | |
| TGGTTCCCCCTATTAGTACA+AGG | - | chr6_1:15051208-15051227 | Msa0876670:CDS | 45.0% | |
| GGTTCCCCCTATTAGTACAA+GGG | - | chr6_1:15051209-15051228 | Msa0876670:CDS | 45.0% | |
| ACAGCCCTTGTACTAATAGG+GGG | + | chr6_1:15051216-15051235 | None:intergenic | 45.0% | |
| AGAGGAAATGGAGGTTGAAG+AGG | - | chr6_1:15051314-15051333 | Msa0876670:intron | 45.0% | |
| GGAGGTTGAAGAGGAAATTG+AGG | - | chr6_1:15051323-15051342 | Msa0876670:intron | 45.0% | |
| GAGGTTGAAGAGGAAGACAA+TGG | - | chr6_1:15051342-15051361 | Msa0876670:intron | 45.0% | |
| GGGTGGCAGTAGTAATGTGT+TGG | - | chr6_1:15051188-15051207 | Msa0876670:CDS | 50.0% | |
| !! | TATAATCGGTTCGAGTCCGG+CGG | - | chr6_1:15051507-15051526 | Msa0876670:intron | 50.0% |
| ! | TTACTATGCAGTGTGGCGGG+TGG | - | chr6_1:15051171-15051190 | Msa0876670:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 15050931 | 15051533 | 15050931 | ID=Msa0876670;Name=Msa0876670 |
| chr6_1 | mRNA | 15050931 | 15051533 | 15050931 | ID=Msa0876670-mRNA-1;Parent=Msa0876670;Name=Msa0876670-mRNA-1;_AED=0.19;_eAED=0.23;_QI=0|0|0|0.5|1|1|2|0|118 |
| chr6_1 | exon | 15051529 | 15051533 | 15051529 | ID=Msa0876670-mRNA-1:exon:4331;Parent=Msa0876670-mRNA-1 |
| chr6_1 | exon | 15050931 | 15051282 | 15050931 | ID=Msa0876670-mRNA-1:exon:4330;Parent=Msa0876670-mRNA-1 |
| chr6_1 | CDS | 15051529 | 15051533 | 15051529 | ID=Msa0876670-mRNA-1:cds;Parent=Msa0876670-mRNA-1 |
| chr6_1 | CDS | 15050931 | 15051282 | 15050931 | ID=Msa0876670-mRNA-1:cds;Parent=Msa0876670-mRNA-1 |
| Gene Sequence |
| Protein sequence |
