AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0880940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0880940 MtrunA17_Chr1g0213011 98.198 222 4 0 1 222 1 222 1.01e-161 445
Msa0880940 MtrunA17_Chr1g0213021 62.896 221 82 0 2 222 5 225 7.70e-98 284
Msa0880940 MtrunA17_Chr1g0213031 61.187 219 85 0 2 220 5 223 8.08e-93 271
Msa0880940 MtrunA17_Chr1g0213041 54.884 215 95 1 4 216 5 219 1.75e-80 240
Msa0880940 MtrunA17_Chr1g0213051 52.804 214 99 1 4 215 5 218 7.94e-78 233
Msa0880940 MtrunA17_Chr7g0224381 50.000 162 81 0 4 165 5 166 4.81e-56 176
Msa0880940 MtrunA17_Chr3g0128761 32.086 187 116 5 29 209 15 196 1.83e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0880940 AT3G55120.1 49.048 210 107 0 4 213 15 224 3.16e-67 207
Msa0880940 AT5G66230.2 42.453 212 114 1 4 215 5 208 1.27e-53 180
Msa0880940 AT5G66220.1 42.767 159 91 0 57 215 1 159 5.51e-44 145
Msa0880940 AT5G05270.1 28.708 209 142 4 8 214 6 209 6.90e-13 65.5
Msa0880940 AT5G05270.2 28.708 209 142 4 8 214 6 209 6.90e-13 65.5

Find 80 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGACCAATTCACCTATTT+TGG 0.093565 6_1:+27785505 None:intergenic
AGCTATGATGTTTCAGATTT+TGG 0.159725 6_1:+27785393 None:intergenic
CAATCACCTGATGGAATATT+AGG 0.209322 6_1:-27784389 Msa0880940:CDS
CACTGCCATAGGTGTTTATT+TGG 0.211608 6_1:-27785204 Msa0880940:CDS
TTGAAATCAGTTGGAACTTA+TGG 0.230001 6_1:-27784497 Msa0880940:CDS
TTCTTGTGCTGCAACGCTTT+TGG 0.248420 6_1:+27784022 None:intergenic
AATTAGAGGGTCAAAGATTA+GGG 0.310626 6_1:-27784573 Msa0880940:CDS
CTTGATGAAGTTTCCTTCAA+TGG 0.330932 6_1:+27785229 None:intergenic
CCCTGATCTTAAGCGCTGTT+TGG 0.332738 6_1:-27784128 Msa0880940:CDS
AATAATTAAGAACAAGAATA+TGG 0.334406 6_1:-27785363 Msa0880940:exon
AACGAGGGTGCTTTCAAGAT+TGG 0.337469 6_1:-27784082 Msa0880940:CDS
TAATTAGAGGGTCAAAGATT+AGG 0.343487 6_1:-27784574 Msa0880940:CDS
AAAATCCAAAATAGGTGAAT+TGG 0.349824 6_1:-27785510 Msa0880940:five_prime_UTR
TCATATTTCCTCGGTGGCGC+TGG 0.355424 6_1:-27785266 Msa0880940:CDS
CCCTTTGAAAAGTTAATTAG+AGG 0.361486 6_1:-27784587 Msa0880940:CDS
AATCACCTGATGGAATATTA+GGG 0.365814 6_1:-27784388 Msa0880940:CDS
TCAAATAAAATACATGCAAC+TGG 0.398245 6_1:-27785487 Msa0880940:five_prime_UTR
GCCAAACAGCGCTTAAGATC+AGG 0.410390 6_1:+27784127 None:intergenic
GCACCAGGTGGAAAATTAAC+AGG 0.410631 6_1:+27784428 None:intergenic
ACCGAGGAAATATGATTTGC+CGG 0.416952 6_1:+27785274 None:intergenic
AGATGATTTACCCTTCCATT+TGG 0.439741 6_1:+27785154 None:intergenic
ACTTGCGTTAAGTGAAGAAC+GGG 0.443654 6_1:+27785421 None:intergenic
GGCAGTTTCATCAGCAGTGT+TGG 0.459798 6_1:-27784179 Msa0880940:CDS
CTCGGTGGCGCTGGGGAGAG+AGG 0.461142 6_1:-27785257 Msa0880940:CDS
CATATTTCCTCGGTGGCGCT+GGG 0.461249 6_1:-27785265 Msa0880940:CDS
GGAACGAAATAAACGCCGCC+TGG 0.467910 6_1:-27785466 Msa0880940:five_prime_UTR
AAATATGATTTGCCGGTGAC+CGG 0.472992 6_1:+27785281 None:intergenic
ACCGGAGAGGTAACCACCGC+TGG 0.477165 6_1:+27785299 None:intergenic
TGAGTACTCAAGGAAGGTTA+TGG 0.487878 6_1:-27784537 Msa0880940:CDS
TAACCACCGCTGGGTATTCA+AGG 0.488480 6_1:+27785309 None:intergenic
GGATACAAGTATACCAGAAA+AGG 0.490457 6_1:-27784224 Msa0880940:CDS
AATAGGAAGCGGTCTGGCCC+AGG 0.492465 6_1:+27785448 None:intergenic
CCCAGCGGTGGTTACCTCTC+CGG 0.492838 6_1:-27785300 Msa0880940:CDS
TATTATTGATACTAATAAGA+AGG 0.499753 6_1:+27783945 None:intergenic
TGGCTTCACTAGCTGCCAAA+TGG 0.503292 6_1:-27785169 Msa0880940:CDS
GTTACAGCTTAGTTTCTCTC+CGG 0.504378 6_1:-27784245 Msa0880940:intron
GAGAGAGGATTGACCATTGA+AGG 0.509487 6_1:-27785242 Msa0880940:CDS
CTAACCCCTAATATTCCATC+AGG 0.511312 6_1:+27784383 None:intergenic
ATCTTCCAAATAAACACCTA+TGG 0.512141 6_1:+27785199 None:intergenic
GTGGTTACCTCTCCGGTCAC+CGG 0.524005 6_1:-27785293 Msa0880940:CDS
TTCACTAGCTGCCAAATGGA+AGG 0.526249 6_1:-27785165 Msa0880940:CDS
CCAAACAGCGCTTAAGATCA+GGG 0.532079 6_1:+27784128 None:intergenic
TTCTACAGGCAATCACCTGA+TGG 0.554083 6_1:-27784398 Msa0880940:CDS
GAACGAAATAAACGCCGCCT+GGG 0.554703 6_1:-27785465 Msa0880940:five_prime_UTR
TGATGTCTCTGTAAAAGTCA+AGG 0.560127 6_1:+27785114 None:intergenic
GACTTGCGTTAAGTGAAGAA+CGG 0.560970 6_1:+27785420 None:intergenic
GGCTGCACTCATAGAGAACA+AGG 0.569164 6_1:-27784200 Msa0880940:CDS
ACCGGCAAATCATATTTCCT+CGG 0.576452 6_1:-27785275 Msa0880940:CDS
TGAGTGGTCCTGAGTACTCA+AGG 0.577740 6_1:-27784547 Msa0880940:CDS
CTCTCTCCCCAGCGCCACCG+AGG 0.582911 6_1:+27785258 None:intergenic
CTGTAGAAAACAGAGGCACC+AGG 0.591901 6_1:+27784413 None:intergenic
GATGTCTCTGTAAAAGTCAA+GGG 0.595362 6_1:+27785115 None:intergenic
CCTTTGAAAAGTTAATTAGA+GGG 0.595496 6_1:-27784586 Msa0880940:CDS
ATTACCTGCGTTGTTGAACG+AGG 0.600190 6_1:-27784098 Msa0880940:CDS
ATAACCTTCCTTGAGTACTC+AGG 0.607676 6_1:+27784539 None:intergenic
TTCATCAAGTTCACTGCCAT+AGG 0.610295 6_1:-27785215 Msa0880940:CDS
GCAGTGTTGGAGACTATGAT+CGG 0.622152 6_1:-27784166 Msa0880940:CDS
AGGAAGCGGTCTGGCCCAGG+CGG 0.623344 6_1:+27785451 None:intergenic
TCACTAGCTGCCAAATGGAA+GGG 0.624312 6_1:-27785164 Msa0880940:CDS
GTGGCACACTTGAAATCAGT+TGG 0.625898 6_1:-27784506 Msa0880940:CDS
CCGGAGAGGTAACCACCGCT+GGG 0.632340 6_1:+27785300 None:intergenic
TACAAGTATACCAGAAAAGG+AGG 0.632960 6_1:-27784221 Msa0880940:CDS
TGGTCCTGAGTACTCAAGGA+AGG 0.639707 6_1:-27784543 Msa0880940:CDS
GGCAAATCATATTTCCTCGG+TGG 0.648065 6_1:-27785272 Msa0880940:CDS
AAGGTTCTCCACAGTGATTG+CGG 0.648293 6_1:+27785328 None:intergenic
ATATTTCCTCGGTGGCGCTG+GGG 0.654176 6_1:-27785264 Msa0880940:CDS
GCACCCTCGTTCAACAACGC+AGG 0.670342 6_1:+27784094 None:intergenic
TGATTTGCCGGTGACCGGAG+AGG 0.671162 6_1:+27785286 None:intergenic
TTATTTGGAAGATATAGCAG+TGG 0.671898 6_1:-27785189 Msa0880940:CDS
GAACCTTGAATACCCAGCGG+TGG 0.677026 6_1:-27785312 Msa0880940:CDS
CAAACAGCGCTTAAGATCAG+GGG 0.682290 6_1:+27784129 None:intergenic
TTACCTGCGTTGTTGAACGA+GGG 0.686961 6_1:-27784097 Msa0880940:CDS
ATCACCTGATGGAATATTAG+GGG 0.689762 6_1:-27784387 Msa0880940:intron
TCAAAGATTAGGGAATTGAG+TGG 0.698363 6_1:-27784563 Msa0880940:CDS
TAGAAAACAGAGGCACCAGG+TGG 0.700225 6_1:+27784416 None:intergenic
GCAGCACAAGAATGAGACCA+TGG 0.722638 6_1:-27784011 Msa0880940:three_prime_UTR
ATCAATCACCGCAATCACTG+TGG 0.724881 6_1:-27785336 Msa0880940:CDS
GAAGGTTATGGAGAACTGTG+TGG 0.734369 6_1:-27784525 Msa0880940:CDS
TGATTGCCTGTAGAAAACAG+AGG 0.767471 6_1:+27784406 None:intergenic
GGAGAACCTTGAATACCCAG+CGG 0.782943 6_1:-27785315 Msa0880940:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTAAGAACAAGAATA+TGG - chr6_1:27783955-27783974 Msa0880940:three_prime_UTR 15.0%
!! AAATCATTTAATTAATTGCA+AGG + chr6_1:27784270-27784289 None:intergenic 15.0%
!! GTAATACTATAGTATTTAAT+TGG + chr6_1:27784586-27784605 None:intergenic 15.0%
!!! TGATAAATTGATAATTTTGT+AGG - chr6_1:27784707-27784726 Msa0880940:intron 15.0%
!!! TTTTAATATACTAGTATTAG+TGG + chr6_1:27785047-27785066 None:intergenic 15.0%
!! TATTATTGATACTAATAAGA+AGG + chr6_1:27785376-27785395 None:intergenic 15.0%
!! AATAATTAAGAACAAGAATA+TGG - chr6_1:27783955-27783974 Msa0880940:three_prime_UTR 15.0%
!! AAATCATTTAATTAATTGCA+AGG + chr6_1:27784270-27784289 None:intergenic 15.0%
!! GTAATACTATAGTATTTAAT+TGG + chr6_1:27784586-27784605 None:intergenic 15.0%
!!! TGATAAATTGATAATTTTGT+AGG - chr6_1:27784707-27784726 Msa0880940:intron 15.0%
!!! TTTTAATATACTAGTATTAG+TGG + chr6_1:27785047-27785066 None:intergenic 15.0%
!! TATTATTGATACTAATAAGA+AGG + chr6_1:27785376-27785395 None:intergenic 15.0%
!! AGAAAAGAAAAATCCAAAAT+AGG - chr6_1:27783800-27783819 Msa0880940:three_prime_UTR 20.0%
!! GTTAGACTTTAATTAATTCT+TGG + chr6_1:27784495-27784514 None:intergenic 20.0%
!! AATTAAAGTCTAACATACAA+CGG - chr6_1:27784501-27784520 Msa0880940:CDS 20.0%
!! AGTTAAGAAACAGAATTAAT+TGG + chr6_1:27784969-27784988 None:intergenic 20.0%
!!! TTTCTTAACTATTTTAGTTG+AGG - chr6_1:27784979-27784998 Msa0880940:intron 20.0%
!! AGAAAAGAAAAATCCAAAAT+AGG - chr6_1:27783800-27783819 Msa0880940:three_prime_UTR 20.0%
!! GTTAGACTTTAATTAATTCT+TGG + chr6_1:27784495-27784514 None:intergenic 20.0%
!! AATTAAAGTCTAACATACAA+CGG - chr6_1:27784501-27784520 Msa0880940:CDS 20.0%
!! AGTTAAGAAACAGAATTAAT+TGG + chr6_1:27784969-27784988 None:intergenic 20.0%
!!! TTTCTTAACTATTTTAGTTG+AGG - chr6_1:27784979-27784998 Msa0880940:intron 20.0%
! AAAATCCAAAATAGGTGAAT+TGG - chr6_1:27783808-27783827 Msa0880940:three_prime_UTR 25.0%
! TCAAATAAAATACATGCAAC+TGG - chr6_1:27783831-27783850 Msa0880940:three_prime_UTR 25.0%
!!! CATTTTTTAACATATGAAGC+TGG - chr6_1:27784325-27784344 Msa0880940:intron 25.0%
! AATGTGAGTAAAAACTATGA+AGG + chr6_1:27784394-27784413 None:intergenic 25.0%
! TCAATAAGACTCATATGATT+TGG + chr6_1:27784460-27784479 None:intergenic 25.0%
! TTTAATTGGATGAATTCAAG+TGG + chr6_1:27784572-27784591 None:intergenic 25.0%
!! CCTCTAATTAACTTTTCAAA+GGG + chr6_1:27784734-27784753 None:intergenic 25.0%
!! CCTTTGAAAAGTTAATTAGA+GGG - chr6_1:27784732-27784751 Msa0880940:intron 25.0%
! ATATACTAGTATTAGTGGAA+AGG + chr6_1:27785042-27785061 None:intergenic 25.0%
! AAAATCCAAAATAGGTGAAT+TGG - chr6_1:27783808-27783827 Msa0880940:three_prime_UTR 25.0%
! TCAAATAAAATACATGCAAC+TGG - chr6_1:27783831-27783850 Msa0880940:three_prime_UTR 25.0%
!!! CATTTTTTAACATATGAAGC+TGG - chr6_1:27784325-27784344 Msa0880940:intron 25.0%
! AATGTGAGTAAAAACTATGA+AGG + chr6_1:27784394-27784413 None:intergenic 25.0%
! TCAATAAGACTCATATGATT+TGG + chr6_1:27784460-27784479 None:intergenic 25.0%
! TTTAATTGGATGAATTCAAG+TGG + chr6_1:27784572-27784591 None:intergenic 25.0%
!! CCTCTAATTAACTTTTCAAA+GGG + chr6_1:27784734-27784753 None:intergenic 25.0%
!! CCTTTGAAAAGTTAATTAGA+GGG - chr6_1:27784732-27784751 Msa0880940:intron 25.0%
! ATATACTAGTATTAGTGGAA+AGG + chr6_1:27785042-27785061 None:intergenic 25.0%
! TTTGACCAATTCACCTATTT+TGG + chr6_1:27783816-27783835 None:intergenic 30.0%
! AGCTATGATGTTTCAGATTT+TGG + chr6_1:27783928-27783947 None:intergenic 30.0%
ATCTTCCAAATAAACACCTA+TGG + chr6_1:27784122-27784141 None:intergenic 30.0%
TTATTTGGAAGATATAGCAG+TGG - chr6_1:27784129-27784148 Msa0880940:CDS 30.0%
!! CTCATATGATTTGGTTGATT+AGG + chr6_1:27784451-27784470 None:intergenic 30.0%
!! AGTCTTATTGATGTAGAGTT+CGG - chr6_1:27784469-27784488 Msa0880940:CDS 30.0%
! CCCTTTGAAAAGTTAATTAG+AGG - chr6_1:27784731-27784750 Msa0880940:intron 30.0%
! CCCTCTAATTAACTTTTCAA+AGG + chr6_1:27784735-27784754 None:intergenic 30.0%
TAATTAGAGGGTCAAAGATT+AGG - chr6_1:27784744-27784763 Msa0880940:intron 30.0%
AATTAGAGGGTCAAAGATTA+GGG - chr6_1:27784745-27784764 Msa0880940:intron 30.0%
TTGAAATCAGTTGGAACTTA+TGG - chr6_1:27784821-27784840 Msa0880940:intron 30.0%
AATCACCTGATGGAATATTA+GGG - chr6_1:27784930-27784949 Msa0880940:intron 30.0%
!! TTTTCTGGTATACTTGTATC+CGG + chr6_1:27785095-27785114 None:intergenic 30.0%
TGTAGACTTAAAAAAGTCCA+TGG + chr6_1:27785327-27785346 None:intergenic 30.0%
! TTTGACCAATTCACCTATTT+TGG + chr6_1:27783816-27783835 None:intergenic 30.0%
! AGCTATGATGTTTCAGATTT+TGG + chr6_1:27783928-27783947 None:intergenic 30.0%
ATCTTCCAAATAAACACCTA+TGG + chr6_1:27784122-27784141 None:intergenic 30.0%
TTATTTGGAAGATATAGCAG+TGG - chr6_1:27784129-27784148 Msa0880940:CDS 30.0%
!! CTCATATGATTTGGTTGATT+AGG + chr6_1:27784451-27784470 None:intergenic 30.0%
!! AGTCTTATTGATGTAGAGTT+CGG - chr6_1:27784469-27784488 Msa0880940:CDS 30.0%
! CCCTTTGAAAAGTTAATTAG+AGG - chr6_1:27784731-27784750 Msa0880940:intron 30.0%
! CCCTCTAATTAACTTTTCAA+AGG + chr6_1:27784735-27784754 None:intergenic 30.0%
TAATTAGAGGGTCAAAGATT+AGG - chr6_1:27784744-27784763 Msa0880940:intron 30.0%
AATTAGAGGGTCAAAGATTA+GGG - chr6_1:27784745-27784764 Msa0880940:intron 30.0%
TTGAAATCAGTTGGAACTTA+TGG - chr6_1:27784821-27784840 Msa0880940:intron 30.0%
AATCACCTGATGGAATATTA+GGG - chr6_1:27784930-27784949 Msa0880940:intron 30.0%
!! TTTTCTGGTATACTTGTATC+CGG + chr6_1:27785095-27785114 None:intergenic 30.0%
TGTAGACTTAAAAAAGTCCA+TGG + chr6_1:27785327-27785346 None:intergenic 30.0%
!! AGTGAAGAACGGGTTTTAAT+AGG + chr6_1:27783890-27783909 None:intergenic 35.0%
CTTGATGAAGTTTCCTTCAA+TGG + chr6_1:27784092-27784111 None:intergenic 35.0%
AGATGATTTACCCTTCCATT+TGG + chr6_1:27784167-27784186 None:intergenic 35.0%
GATGTCTCTGTAAAAGTCAA+GGG + chr6_1:27784206-27784225 None:intergenic 35.0%
TGATGTCTCTGTAAAAGTCA+AGG + chr6_1:27784207-27784226 None:intergenic 35.0%
! TTTTACAGAGACATCATCTC+AGG - chr6_1:27784211-27784230 Msa0880940:CDS 35.0%
TCAAAGATTAGGGAATTGAG+TGG - chr6_1:27784755-27784774 Msa0880940:intron 35.0%
CAATCACCTGATGGAATATT+AGG - chr6_1:27784929-27784948 Msa0880940:intron 35.0%
ATCACCTGATGGAATATTAG+GGG - chr6_1:27784931-27784950 Msa0880940:intron 35.0%
GGATACAAGTATACCAGAAA+AGG - chr6_1:27785094-27785113 Msa0880940:intron 35.0%
TACAAGTATACCAGAAAAGG+AGG - chr6_1:27785097-27785116 Msa0880940:intron 35.0%
!! AGTGAAGAACGGGTTTTAAT+AGG + chr6_1:27783890-27783909 None:intergenic 35.0%
CTTGATGAAGTTTCCTTCAA+TGG + chr6_1:27784092-27784111 None:intergenic 35.0%
AGATGATTTACCCTTCCATT+TGG + chr6_1:27784167-27784186 None:intergenic 35.0%
GATGTCTCTGTAAAAGTCAA+GGG + chr6_1:27784206-27784225 None:intergenic 35.0%
TGATGTCTCTGTAAAAGTCA+AGG + chr6_1:27784207-27784226 None:intergenic 35.0%
! TTTTACAGAGACATCATCTC+AGG - chr6_1:27784211-27784230 Msa0880940:CDS 35.0%
TCAAAGATTAGGGAATTGAG+TGG - chr6_1:27784755-27784774 Msa0880940:intron 35.0%
CAATCACCTGATGGAATATT+AGG - chr6_1:27784929-27784948 Msa0880940:intron 35.0%
ATCACCTGATGGAATATTAG+GGG - chr6_1:27784931-27784950 Msa0880940:intron 35.0%
GGATACAAGTATACCAGAAA+AGG - chr6_1:27785094-27785113 Msa0880940:intron 35.0%
TACAAGTATACCAGAAAAGG+AGG - chr6_1:27785097-27785116 Msa0880940:intron 35.0%
!! GAACGGGTTTTAATAGGAAG+CGG + chr6_1:27783884-27783903 None:intergenic 40.0%
ACTTGCGTTAAGTGAAGAAC+GGG + chr6_1:27783900-27783919 None:intergenic 40.0%
GACTTGCGTTAAGTGAAGAA+CGG + chr6_1:27783901-27783920 None:intergenic 40.0%
! AAATATGATTTGCCGGTGAC+CGG + chr6_1:27784040-27784059 None:intergenic 40.0%
ACCGGCAAATCATATTTCCT+CGG - chr6_1:27784043-27784062 Msa0880940:three_prime_UTR 40.0%
! ACCGAGGAAATATGATTTGC+CGG + chr6_1:27784047-27784066 None:intergenic 40.0%
TTCATCAAGTTCACTGCCAT+AGG - chr6_1:27784103-27784122 Msa0880940:CDS 40.0%
CACTGCCATAGGTGTTTATT+TGG - chr6_1:27784114-27784133 Msa0880940:CDS 40.0%
ATAACCTTCCTTGAGTACTC+AGG + chr6_1:27784782-27784801 None:intergenic 40.0%
TGAGTACTCAAGGAAGGTTA+TGG - chr6_1:27784781-27784800 Msa0880940:intron 40.0%
! AAGCCTGTTAATTTTCCACC+TGG - chr6_1:27784887-27784906 Msa0880940:intron 40.0%
TGATTGCCTGTAGAAAACAG+AGG + chr6_1:27784915-27784934 None:intergenic 40.0%
CTAACCCCTAATATTCCATC+AGG + chr6_1:27784938-27784957 None:intergenic 40.0%
GTTACAGCTTAGTTTCTCTC+CGG - chr6_1:27785073-27785092 Msa0880940:intron 40.0%
TTGAGTAGAGTTGCTTGATG+AGG - chr6_1:27785528-27785547 Msa0880940:five_prime_UTR 40.0%
!! GAACGGGTTTTAATAGGAAG+CGG + chr6_1:27783884-27783903 None:intergenic 40.0%
ACTTGCGTTAAGTGAAGAAC+GGG + chr6_1:27783900-27783919 None:intergenic 40.0%
GACTTGCGTTAAGTGAAGAA+CGG + chr6_1:27783901-27783920 None:intergenic 40.0%
! AAATATGATTTGCCGGTGAC+CGG + chr6_1:27784040-27784059 None:intergenic 40.0%
ACCGGCAAATCATATTTCCT+CGG - chr6_1:27784043-27784062 Msa0880940:three_prime_UTR 40.0%
! ACCGAGGAAATATGATTTGC+CGG + chr6_1:27784047-27784066 None:intergenic 40.0%
TTCATCAAGTTCACTGCCAT+AGG - chr6_1:27784103-27784122 Msa0880940:CDS 40.0%
CACTGCCATAGGTGTTTATT+TGG - chr6_1:27784114-27784133 Msa0880940:CDS 40.0%
ATAACCTTCCTTGAGTACTC+AGG + chr6_1:27784782-27784801 None:intergenic 40.0%
TGAGTACTCAAGGAAGGTTA+TGG - chr6_1:27784781-27784800 Msa0880940:intron 40.0%
! AAGCCTGTTAATTTTCCACC+TGG - chr6_1:27784887-27784906 Msa0880940:intron 40.0%
TGATTGCCTGTAGAAAACAG+AGG + chr6_1:27784915-27784934 None:intergenic 40.0%
CTAACCCCTAATATTCCATC+AGG + chr6_1:27784938-27784957 None:intergenic 40.0%
GTTACAGCTTAGTTTCTCTC+CGG - chr6_1:27785073-27785092 Msa0880940:intron 40.0%
!! GGTTTTAATAGGAAGCGGTC+TGG + chr6_1:27783879-27783898 None:intergenic 45.0%
ATCAATCACCGCAATCACTG+TGG - chr6_1:27783982-27784001 Msa0880940:three_prime_UTR 45.0%
AAGGTTCTCCACAGTGATTG+CGG + chr6_1:27783993-27784012 None:intergenic 45.0%
GGCAAATCATATTTCCTCGG+TGG - chr6_1:27784046-27784065 Msa0880940:three_prime_UTR 45.0%
GAGAGAGGATTGACCATTGA+AGG - chr6_1:27784076-27784095 Msa0880940:CDS 45.0%
TTCACTAGCTGCCAAATGGA+AGG - chr6_1:27784153-27784172 Msa0880940:CDS 45.0%
TCACTAGCTGCCAAATGGAA+GGG - chr6_1:27784154-27784173 Msa0880940:CDS 45.0%
GAAGGTTATGGAGAACTGTG+TGG - chr6_1:27784793-27784812 Msa0880940:intron 45.0%
GTGGCACACTTGAAATCAGT+TGG - chr6_1:27784812-27784831 Msa0880940:intron 45.0%
GCACCAGGTGGAAAATTAAC+AGG + chr6_1:27784893-27784912 None:intergenic 45.0%
TTCTACAGGCAATCACCTGA+TGG - chr6_1:27784920-27784939 Msa0880940:intron 45.0%
! GCAGTGTTGGAGACTATGAT+CGG - chr6_1:27785152-27785171 Msa0880940:CDS 45.0%
CAAACAGCGCTTAAGATCAG+GGG + chr6_1:27785192-27785211 None:intergenic 45.0%
CCAAACAGCGCTTAAGATCA+GGG + chr6_1:27785193-27785212 None:intergenic 45.0%
ATTACCTGCGTTGTTGAACG+AGG - chr6_1:27785220-27785239 Msa0880940:CDS 45.0%
TTACCTGCGTTGTTGAACGA+GGG - chr6_1:27785221-27785240 Msa0880940:CDS 45.0%
!! AACGAGGGTGCTTTCAAGAT+TGG - chr6_1:27785236-27785255 Msa0880940:CDS 45.0%
! TTCTTGTGCTGCAACGCTTT+TGG + chr6_1:27785299-27785318 None:intergenic 45.0%
!! GGTTTTAATAGGAAGCGGTC+TGG + chr6_1:27783879-27783898 None:intergenic 45.0%
ATCAATCACCGCAATCACTG+TGG - chr6_1:27783982-27784001 Msa0880940:three_prime_UTR 45.0%
AAGGTTCTCCACAGTGATTG+CGG + chr6_1:27783993-27784012 None:intergenic 45.0%
GGCAAATCATATTTCCTCGG+TGG - chr6_1:27784046-27784065 Msa0880940:three_prime_UTR 45.0%
GAGAGAGGATTGACCATTGA+AGG - chr6_1:27784076-27784095 Msa0880940:CDS 45.0%
TTCACTAGCTGCCAAATGGA+AGG - chr6_1:27784153-27784172 Msa0880940:CDS 45.0%
TCACTAGCTGCCAAATGGAA+GGG - chr6_1:27784154-27784173 Msa0880940:CDS 45.0%
GAAGGTTATGGAGAACTGTG+TGG - chr6_1:27784793-27784812 Msa0880940:intron 45.0%
GTGGCACACTTGAAATCAGT+TGG - chr6_1:27784812-27784831 Msa0880940:intron 45.0%
GCACCAGGTGGAAAATTAAC+AGG + chr6_1:27784893-27784912 None:intergenic 45.0%
TTCTACAGGCAATCACCTGA+TGG - chr6_1:27784920-27784939 Msa0880940:intron 45.0%
! GCAGTGTTGGAGACTATGAT+CGG - chr6_1:27785152-27785171 Msa0880940:CDS 45.0%
CAAACAGCGCTTAAGATCAG+GGG + chr6_1:27785192-27785211 None:intergenic 45.0%
CCAAACAGCGCTTAAGATCA+GGG + chr6_1:27785193-27785212 None:intergenic 45.0%
ATTACCTGCGTTGTTGAACG+AGG - chr6_1:27785220-27785239 Msa0880940:CDS 45.0%
TTACCTGCGTTGTTGAACGA+GGG - chr6_1:27785221-27785240 Msa0880940:CDS 45.0%
!! AACGAGGGTGCTTTCAAGAT+TGG - chr6_1:27785236-27785255 Msa0880940:CDS 45.0%
! TTCTTGTGCTGCAACGCTTT+TGG + chr6_1:27785299-27785318 None:intergenic 45.0%
GAACGAAATAAACGCCGCCT+GGG - chr6_1:27783853-27783872 Msa0880940:three_prime_UTR 50.0%
GGAGAACCTTGAATACCCAG+CGG - chr6_1:27784003-27784022 Msa0880940:three_prime_UTR 50.0%
TAACCACCGCTGGGTATTCA+AGG + chr6_1:27784012-27784031 None:intergenic 50.0%
TGGCTTCACTAGCTGCCAAA+TGG - chr6_1:27784149-27784168 Msa0880940:CDS 50.0%
GACAGACACTCCTCAATCCT+CGG + chr6_1:27784372-27784391 None:intergenic 50.0%
TGAGTGGTCCTGAGTACTCA+AGG - chr6_1:27784771-27784790 Msa0880940:intron 50.0%
TGGTCCTGAGTACTCAAGGA+AGG - chr6_1:27784775-27784794 Msa0880940:intron 50.0%
!! TAGAAAACAGAGGCACCAGG+TGG + chr6_1:27784905-27784924 None:intergenic 50.0%
!! CTGTAGAAAACAGAGGCACC+AGG + chr6_1:27784908-27784927 None:intergenic 50.0%
! CTGGTGCCTCTGTTTTCTAC+AGG - chr6_1:27784906-27784925 Msa0880940:intron 50.0%
! ATGAGTGCAGCCTCCTTTTC+TGG + chr6_1:27785110-27785129 None:intergenic 50.0%
GGCTGCACTCATAGAGAACA+AGG - chr6_1:27785118-27785137 Msa0880940:CDS 50.0%
GGCAGTTTCATCAGCAGTGT+TGG - chr6_1:27785139-27785158 Msa0880940:CDS 50.0%
! CCCTGATCTTAAGCGCTGTT+TGG - chr6_1:27785190-27785209 Msa0880940:CDS 50.0%
GCCAAACAGCGCTTAAGATC+AGG + chr6_1:27785194-27785213 None:intergenic 50.0%
GCAGCACAAGAATGAGACCA+TGG - chr6_1:27785307-27785326 Msa0880940:CDS 50.0%
GAACGAAATAAACGCCGCCT+GGG - chr6_1:27783853-27783872 Msa0880940:three_prime_UTR 50.0%
GGAGAACCTTGAATACCCAG+CGG - chr6_1:27784003-27784022 Msa0880940:three_prime_UTR 50.0%
TAACCACCGCTGGGTATTCA+AGG + chr6_1:27784012-27784031 None:intergenic 50.0%
TGGCTTCACTAGCTGCCAAA+TGG - chr6_1:27784149-27784168 Msa0880940:CDS 50.0%
GACAGACACTCCTCAATCCT+CGG + chr6_1:27784372-27784391 None:intergenic 50.0%
TGAGTGGTCCTGAGTACTCA+AGG - chr6_1:27784771-27784790 Msa0880940:intron 50.0%
TGGTCCTGAGTACTCAAGGA+AGG - chr6_1:27784775-27784794 Msa0880940:intron 50.0%
!! TAGAAAACAGAGGCACCAGG+TGG + chr6_1:27784905-27784924 None:intergenic 50.0%
!! CTGTAGAAAACAGAGGCACC+AGG + chr6_1:27784908-27784927 None:intergenic 50.0%
! CTGGTGCCTCTGTTTTCTAC+AGG - chr6_1:27784906-27784925 Msa0880940:intron 50.0%
! ATGAGTGCAGCCTCCTTTTC+TGG + chr6_1:27785110-27785129 None:intergenic 50.0%
GGCTGCACTCATAGAGAACA+AGG - chr6_1:27785118-27785137 Msa0880940:CDS 50.0%
GGCAGTTTCATCAGCAGTGT+TGG - chr6_1:27785139-27785158 Msa0880940:CDS 50.0%
! CCCTGATCTTAAGCGCTGTT+TGG - chr6_1:27785190-27785209 Msa0880940:CDS 50.0%
GCCAAACAGCGCTTAAGATC+AGG + chr6_1:27785194-27785213 None:intergenic 50.0%
GCAGCACAAGAATGAGACCA+TGG - chr6_1:27785307-27785326 Msa0880940:CDS 50.0%
GGAACGAAATAAACGCCGCC+TGG - chr6_1:27783852-27783871 Msa0880940:three_prime_UTR 55.0%
GAACCTTGAATACCCAGCGG+TGG - chr6_1:27784006-27784025 Msa0880940:three_prime_UTR 55.0%
TCATATTTCCTCGGTGGCGC+TGG - chr6_1:27784052-27784071 Msa0880940:three_prime_UTR 55.0%
CATATTTCCTCGGTGGCGCT+GGG - chr6_1:27784053-27784072 Msa0880940:three_prime_UTR 55.0%
ATATTTCCTCGGTGGCGCTG+GGG - chr6_1:27784054-27784073 Msa0880940:three_prime_UTR 55.0%
GACACATGTCCTCTGATCCG+AGG - chr6_1:27784352-27784371 Msa0880940:intron 55.0%
GTCCTCTGATCCGAGGATTG+AGG - chr6_1:27784359-27784378 Msa0880940:intron 55.0%
CTCCTCAATCCTCGGATCAG+AGG + chr6_1:27784364-27784383 None:intergenic 55.0%
GGAACGAAATAAACGCCGCC+TGG - chr6_1:27783852-27783871 Msa0880940:three_prime_UTR 55.0%
GAACCTTGAATACCCAGCGG+TGG - chr6_1:27784006-27784025 Msa0880940:three_prime_UTR 55.0%
TCATATTTCCTCGGTGGCGC+TGG - chr6_1:27784052-27784071 Msa0880940:three_prime_UTR 55.0%
CATATTTCCTCGGTGGCGCT+GGG - chr6_1:27784053-27784072 Msa0880940:three_prime_UTR 55.0%
ATATTTCCTCGGTGGCGCTG+GGG - chr6_1:27784054-27784073 Msa0880940:three_prime_UTR 55.0%
GACACATGTCCTCTGATCCG+AGG - chr6_1:27784352-27784371 Msa0880940:intron 55.0%
GTCCTCTGATCCGAGGATTG+AGG - chr6_1:27784359-27784378 Msa0880940:intron 55.0%
CTCCTCAATCCTCGGATCAG+AGG + chr6_1:27784364-27784383 None:intergenic 55.0%
AATAGGAAGCGGTCTGGCCC+AGG + chr6_1:27783873-27783892 None:intergenic 60.0%
GTGGTTACCTCTCCGGTCAC+CGG - chr6_1:27784025-27784044 Msa0880940:three_prime_UTR 60.0%
! TGATTTGCCGGTGACCGGAG+AGG + chr6_1:27784035-27784054 None:intergenic 60.0%
GCACCCTCGTTCAACAACGC+AGG + chr6_1:27785227-27785246 None:intergenic 60.0%
AATAGGAAGCGGTCTGGCCC+AGG + chr6_1:27783873-27783892 None:intergenic 60.0%
GTGGTTACCTCTCCGGTCAC+CGG - chr6_1:27784025-27784044 Msa0880940:three_prime_UTR 60.0%
! TGATTTGCCGGTGACCGGAG+AGG + chr6_1:27784035-27784054 None:intergenic 60.0%
GCACCCTCGTTCAACAACGC+AGG + chr6_1:27785227-27785246 None:intergenic 60.0%
CCGGAGAGGTAACCACCGCT+GGG + chr6_1:27784021-27784040 None:intergenic 65.0%
CCCAGCGGTGGTTACCTCTC+CGG - chr6_1:27784018-27784037 Msa0880940:three_prime_UTR 65.0%
ACCGGAGAGGTAACCACCGC+TGG + chr6_1:27784022-27784041 None:intergenic 65.0%
CCGGAGAGGTAACCACCGCT+GGG + chr6_1:27784021-27784040 None:intergenic 65.0%
CCCAGCGGTGGTTACCTCTC+CGG - chr6_1:27784018-27784037 Msa0880940:three_prime_UTR 65.0%
ACCGGAGAGGTAACCACCGC+TGG + chr6_1:27784022-27784041 None:intergenic 65.0%
AGGAAGCGGTCTGGCCCAGG+CGG + chr6_1:27783870-27783889 None:intergenic 70.0%
AGGAAGCGGTCTGGCCCAGG+CGG + chr6_1:27783870-27783889 None:intergenic 70.0%
CTCTCTCCCCAGCGCCACCG+AGG + chr6_1:27784063-27784082 None:intergenic 75.0%
! CTCGGTGGCGCTGGGGAGAG+AGG - chr6_1:27784061-27784080 Msa0880940:exon 75.0%
CTCTCTCCCCAGCGCCACCG+AGG + chr6_1:27784063-27784082 None:intergenic 75.0%
! CTCGGTGGCGCTGGGGAGAG+AGG - chr6_1:27784061-27784080 Msa0880940:exon 75.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 27783792 27785548 27783792 ID=Msa0880940;Name=Msa0880940
chr6_1 mRNA 27783792 27785548 27783792 ID=Msa0880940-mRNA-1;Parent=Msa0880940;Name=Msa0880940-mRNA-1;_AED=0.40;_eAED=0.40;_QI=182|1|1|1|1|1|3|283|222
chr6_1 exon 27783792 27784260 27783792 ID=Msa0880940-mRNA-1:exon:6876;Parent=Msa0880940-mRNA-1
chr6_1 exon 27784388 27784611 27784388 ID=Msa0880940-mRNA-1:exon:6875;Parent=Msa0880940-mRNA-1
chr6_1 exon 27785108 27785548 27785108 ID=Msa0880940-mRNA-1:exon:6874;Parent=Msa0880940-mRNA-1
chr6_1 five_prime_UTR 27785367 27785548 27785367 ID=Msa0880940-mRNA-1:five_prime_utr;Parent=Msa0880940-mRNA-1
chr6_1 CDS 27785108 27785366 27785108 ID=Msa0880940-mRNA-1:cds;Parent=Msa0880940-mRNA-1
chr6_1 CDS 27784388 27784611 27784388 ID=Msa0880940-mRNA-1:cds;Parent=Msa0880940-mRNA-1
chr6_1 CDS 27784075 27784260 27784075 ID=Msa0880940-mRNA-1:cds;Parent=Msa0880940-mRNA-1
chr6_1 three_prime_UTR 27783792 27784074 27783792 ID=Msa0880940-mRNA-1:three_prime_utr;Parent=Msa0880940-mRNA-1
Gene Sequence

>Msa0880940

ATGGCTGCATCAATCACCGCAATCACTGTGGAGAACCTTGAATACCCAGCGGTGGTTACCTCTCCGGTCACCGGCAAATCATATTTCCTCGGTGGCGCTGGGGAGAGAGGATTGACCATTGAAGGAAACTTCATCAAGTTCACTGCCATAGGTGTTTATTTGGAAGATATAGCAGTGGCTTCACTAGCTGCCAAATGGAAGGGTAAATCATCTGAAGAGTTACTTGAGACCCTTGACTTTTACAGAGACATCATCTCAGGTCCCTTTGAAAAGTTAATTAGAGGGTCAAAGATTAGGGAATTGAGTGGTCCTGAGTACTCAAGGAAGGTTATGGAGAACTGTGTGGCACACTTGAAATCAGTTGGAACTTATGGAGATGCAGAAGCTGAAGCTATGCAAAAATTTGCTGAAGCTTTCAAGCCTGTTAATTTTCCACCTGGTGCCTCTGTTTTCTACAGGCAATCACCTGATGGAATATTAGGGCTTAGTTTCTCTCCGGATACAAGTATACCAGAAAAGGAGGCTGCACTCATAGAGAACAAGGCAGTTTCATCAGCAGTGTTGGAGACTATGATCGGCGAACACGCTGTTTCCCCTGATCTTAAGCGCTGTTTGGCTGCAAGATTACCTGCGTTGTTGAACGAGGGTGCTTTCAAGATTGGAAACTGA
Protein sequence

>Msa0880940

MAASITAITVENLEYPAVVTSPVTGKSYFLGGAGERGLTIEGNFIKFTAIGVYLEDIAVASLAAKWKGKSSEELLETLDFYRDIISGPFEKLIRGSKIRELSGPEYSRKVMENCVAHLKSVGTYGDAEAEAMQKFAEAFKPVNFPPGASVFYRQSPDGILGLSFSPDTSIPEKEAALIENKAVSSAVLETMIGEHAVSPDLKRCLAARLPALLNEGAFKIGN*