Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0072830 | Msa0884820 | 0.815212 | 1.037938e-51 | -8.615850e-47 |
| Msa0072860 | Msa0884820 | 0.808897 | 2.471013e-50 | -8.615850e-47 |
| Msa0416060 | Msa0884820 | 0.807793 | 4.249051e-50 | -8.615850e-47 |
| Msa0884820 | Msa1096510 | 0.801134 | 1.038885e-48 | -8.615850e-47 |
| Msa0884820 | Msa1248220 | 0.808027 | 3.788990e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAATTGCTGCTGGGATTC+TGG | 0.285120 | 6_1:+38957207 | None:intergenic |
| CCGAATACAGTATTTGATTC+TGG | 0.287519 | 6_1:+38957459 | None:intergenic |
| ACATTCCGTCAATGTCATAT+AGG | 0.377716 | 6_1:-38957504 | Msa0884820:CDS |
| ACTTCAATTGCCAGCTAACT+TGG | 0.390370 | 6_1:-38957187 | Msa0884820:three_prime_UTR |
| CATTCCGTCAATGTCATATA+GGG | 0.395902 | 6_1:-38957503 | Msa0884820:CDS |
| CCAGAATCAAATACTGTATT+CGG | 0.396007 | 6_1:-38957459 | Msa0884820:CDS |
| CTGCTGCGAGAACTATCAAA+AGG | 0.443736 | 6_1:-38957143 | Msa0884820:three_prime_UTR |
| TACAGTATTTGATTCTGGTC+GGG | 0.463595 | 6_1:+38957464 | None:intergenic |
| AACTGTTGAACCAAGTTAGC+TGG | 0.464113 | 6_1:+38957177 | None:intergenic |
| GCAATTGAAGTAATTGCTGC+TGG | 0.485971 | 6_1:+38957199 | None:intergenic |
| AACAGGAAGAATGTCTTGAA+TGG | 0.494577 | 6_1:+38957403 | None:intergenic |
| CTCTCCCTATATGACATTGA+CGG | 0.498527 | 6_1:+38957499 | None:intergenic |
| TCAGGAATGACGGAAAGAAC+AGG | 0.508934 | 6_1:+38957386 | None:intergenic |
| GATAGTTCTCGCAGCAGCGC+GGG | 0.517491 | 6_1:+38957149 | None:intergenic |
| TAGATTTACCTCAGGAATGA+CGG | 0.561552 | 6_1:+38957376 | None:intergenic |
| ATACAGTATTTGATTCTGGT+CGG | 0.566488 | 6_1:+38957463 | None:intergenic |
| CGTCAATGTCATATAGGGAG+AGG | 0.574228 | 6_1:-38957498 | Msa0884820:CDS |
| TGTTCTTTCCGTCATTCCTG+AGG | 0.588868 | 6_1:-38957384 | Msa0884820:intron |
| TGCTGCGAGAACTATCAAAA+GGG | 0.593150 | 6_1:-38957142 | None:intergenic |
| CAATTGAAGTAATTGCTGCT+GGG | 0.594992 | 6_1:+38957200 | None:intergenic |
| ATTGCTGCTGGGATTCTGGT+GGG | 0.596453 | 6_1:+38957211 | None:intergenic |
| TGTCATATAGGGAGAGGCAA+AGG | 0.609167 | 6_1:-38957492 | Msa0884820:CDS |
| AATTGCTGCTGGGATTCTGG+TGG | 0.630893 | 6_1:+38957210 | None:intergenic |
| TGATAGTTCTCGCAGCAGCG+CGG | 0.673188 | 6_1:+38957148 | None:intergenic |
| GAATCAAATACTGTATTCGG+AGG | 0.674701 | 6_1:-38957456 | Msa0884820:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATACAGTATTTGATTCTGGT+CGG | + | chr6_1:38957210-38957229 | None:intergenic | 30.0% | |
| CCAGAATCAAATACTGTATT+CGG | - | chr6_1:38957211-38957230 | Msa0884820:three_prime_UTR | 30.0% | |
| GTCATAAGCAATTACACATA+TGG | - | chr6_1:38957388-38957407 | Msa0884820:three_prime_UTR | 30.0% | |
| ACATTCCGTCAATGTCATAT+AGG | - | chr6_1:38957166-38957185 | Msa0884820:three_prime_UTR | 35.0% | |
| CATTCCGTCAATGTCATATA+GGG | - | chr6_1:38957167-38957186 | Msa0884820:three_prime_UTR | 35.0% | |
| TACAGTATTTGATTCTGGTC+GGG | + | chr6_1:38957209-38957228 | None:intergenic | 35.0% | |
| CCGAATACAGTATTTGATTC+TGG | + | chr6_1:38957214-38957233 | None:intergenic | 35.0% | |
| GAATCAAATACTGTATTCGG+AGG | - | chr6_1:38957214-38957233 | Msa0884820:three_prime_UTR | 35.0% | |
| AACAGGAAGAATGTCTTGAA+TGG | + | chr6_1:38957270-38957289 | None:intergenic | 35.0% | |
| TAGATTTACCTCAGGAATGA+CGG | + | chr6_1:38957297-38957316 | None:intergenic | 35.0% | |
| CAAATGCTTAGATTTACCTC+AGG | + | chr6_1:38957305-38957324 | None:intergenic | 35.0% | |
| ! | TATTAGTGAACATGGTTCTG+CGG | + | chr6_1:38957349-38957368 | None:intergenic | 35.0% |
| TTCGTCTGTATTAGTGAACA+TGG | + | chr6_1:38957357-38957376 | None:intergenic | 35.0% | |
| CAATTGAAGTAATTGCTGCT+GGG | + | chr6_1:38957473-38957492 | None:intergenic | 35.0% | |
| CTCTCCCTATATGACATTGA+CGG | + | chr6_1:38957174-38957193 | None:intergenic | 40.0% | |
| GCAATTGAAGTAATTGCTGC+TGG | + | chr6_1:38957474-38957493 | None:intergenic | 40.0% | |
| ACTTCAATTGCCAGCTAACT+TGG | - | chr6_1:38957483-38957502 | Msa0884820:CDS | 40.0% | |
| AACTGTTGAACCAAGTTAGC+TGG | + | chr6_1:38957496-38957515 | None:intergenic | 40.0% | |
| CGTCAATGTCATATAGGGAG+AGG | - | chr6_1:38957172-38957191 | Msa0884820:three_prime_UTR | 45.0% | |
| TGTCATATAGGGAGAGGCAA+AGG | - | chr6_1:38957178-38957197 | Msa0884820:three_prime_UTR | 45.0% | |
| ! | TCAGGAATGACGGAAAGAAC+AGG | + | chr6_1:38957287-38957306 | None:intergenic | 45.0% |
| TGTTCTTTCCGTCATTCCTG+AGG | - | chr6_1:38957286-38957305 | Msa0884820:intron | 45.0% | |
| AGTAATTGCTGCTGGGATTC+TGG | + | chr6_1:38957466-38957485 | None:intergenic | 45.0% | |
| ATTGCTGCTGGGATTCTGGT+GGG | + | chr6_1:38957462-38957481 | None:intergenic | 50.0% | |
| AATTGCTGCTGGGATTCTGG+TGG | + | chr6_1:38957463-38957482 | None:intergenic | 50.0% | |
| TGATAGTTCTCGCAGCAGCG+CGG | + | chr6_1:38957525-38957544 | None:intergenic | 55.0% | |
| GATAGTTCTCGCAGCAGCGC+GGG | + | chr6_1:38957524-38957543 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 38957143 | 38957549 | 38957143 | ID=Msa0884820;Name=Msa0884820 |
| chr6_1 | mRNA | 38957143 | 38957549 | 38957143 | ID=Msa0884820-mRNA-1;Parent=Msa0884820;Name=Msa0884820-mRNA-1;_AED=0.05;_eAED=0.05;_QI=7|1|0.5|1|1|1|2|152|32 |
| chr6_1 | exon | 38957143 | 38957235 | 38957143 | ID=Msa0884820-mRNA-1:exon:9004;Parent=Msa0884820-mRNA-1 |
| chr6_1 | exon | 38957385 | 38957549 | 38957385 | ID=Msa0884820-mRNA-1:exon:9003;Parent=Msa0884820-mRNA-1 |
| chr6_1 | five_prime_UTR | 38957543 | 38957549 | 38957543 | ID=Msa0884820-mRNA-1:five_prime_utr;Parent=Msa0884820-mRNA-1 |
| chr6_1 | CDS | 38957444 | 38957542 | 38957444 | ID=Msa0884820-mRNA-1:cds;Parent=Msa0884820-mRNA-1 |
| chr6_1 | three_prime_UTR | 38957385 | 38957443 | 38957385 | ID=Msa0884820-mRNA-1:three_prime_utr;Parent=Msa0884820-mRNA-1 |
| chr6_1 | three_prime_UTR | 38957143 | 38957235 | 38957143 | ID=Msa0884820-mRNA-1:three_prime_utr;Parent=Msa0884820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
