AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0889310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0889310 MtrunA17_Chr6g0483981 70.635 126 35 1 16 139 1 126 1.93e-54 179
Msa0889310 MtrunA17_Chr4g0064041 62.687 134 48 2 16 147 1 134 5.54e-51 166
Msa0889310 MtrunA17_Chr4g0064041 55.000 100 45 0 41 140 190 289 3.27e-34 122
Msa0889310 MtrunA17_Chr2g0306461 46.154 117 61 2 23 139 9 123 7.17e-28 102
Msa0889310 MtrunA17_Chr5g0394321 37.903 124 75 1 24 147 8 129 1.70e-25 95.5
Msa0889310 MtrunA17_Chr4g0033131 35.833 120 75 1 23 140 9 128 2.44e-24 92.4
Msa0889310 MtrunA17_Chr4g0033531 35.833 120 75 1 23 140 9 128 2.44e-24 92.4
Msa0889310 MtrunA17_Chr1g0205701 35.200 125 77 2 21 145 6 126 7.31e-24 91.7
Msa0889310 MtrunA17_Chr3g0132441 40.336 119 66 3 21 139 5 118 3.26e-23 89.7
Msa0889310 MtrunA17_Chr2g0301431 39.167 120 63 4 23 140 12 123 4.99e-22 85.1
Msa0889310 MtrunA17_Chr8g0335531 40.650 123 66 4 23 143 5 122 1.23e-21 85.9
Msa0889310 MtrunA17_Chr3g0132431 37.607 117 68 3 21 137 5 116 5.84e-20 81.3
Msa0889310 MtrunA17_Chr8g0335571 39.024 123 68 3 23 143 5 122 6.46e-20 81.6
Msa0889310 MtrunA17_Chr3g0132701 36.364 121 72 2 20 139 4 120 9.64e-20 81.6
Msa0889310 MtrunA17_Chr3g0132511 36.667 120 71 2 21 139 5 120 1.17e-19 81.3
Msa0889310 MtrunA17_Chr2g0323021 44.304 79 43 1 62 140 42 119 1.52e-19 81.3
Msa0889310 MtrunA17_Chr8g0380901 37.288 118 64 3 23 140 12 119 4.13e-19 77.4
Msa0889310 MtrunA17_Chr8g0380711 35.294 102 60 1 39 140 25 120 6.96e-19 77.0
Msa0889310 MtrunA17_Chr7g0253631 36.885 122 72 3 20 140 3 120 7.81e-19 79.7
Msa0889310 MtrunA17_Chr1g0177341 34.454 119 70 3 23 140 9 120 1.24e-18 77.0
Msa0889310 MtrunA17_Chr1g0177331 34.454 119 70 3 23 140 9 120 1.24e-18 77.0
Msa0889310 MtrunA17_Chr7g0253681 39.216 102 57 2 39 139 24 121 1.30e-18 77.4
Msa0889310 MtrunA17_Chr7g0253641 36.441 118 70 2 24 140 7 120 4.24e-18 77.4
Msa0889310 MtrunA17_Chr7g0253761 35.897 117 70 2 24 139 7 119 4.46e-18 76.3
Msa0889310 MtrunA17_Chr7g0253701 36.937 111 65 2 30 139 14 120 5.74e-18 75.5
Msa0889310 MtrunA17_Chr7g0253671 36.937 111 65 2 30 139 14 120 5.74e-18 75.5
Msa0889310 MtrunA17_Chr4g0064031 38.318 107 62 4 39 142 25 130 1.24e-17 74.7
Msa0889310 MtrunA17_Chr7g0253731 32.759 116 74 1 20 135 3 114 1.47e-17 74.7
Msa0889310 MtrunA17_Chr3g0125761 33.051 118 72 2 23 140 14 124 5.32e-17 72.0
Msa0889310 MtrunA17_Chr3g0125791 35.644 101 58 2 39 139 30 123 7.99e-17 71.6
Msa0889310 MtrunA17_Chr1g0196801 40.594 101 55 2 39 139 20 115 2.23e-16 72.0
Msa0889310 MtrunA17_Chr8g0387804 30.769 117 80 1 23 139 10 125 9.55e-16 68.9
Msa0889310 MtrunA17_Chr1g0211101 31.933 119 76 2 23 140 14 128 1.20e-15 70.1
Msa0889310 MtrunA17_Chr2g0331181 35.922 103 63 2 39 140 24 124 2.41e-15 69.3
Msa0889310 MtrunA17_Chr1g0188981 34.343 99 64 1 42 139 29 127 7.93e-15 68.2
Msa0889310 MtrunA17_Chr1g0150131 32.231 121 80 2 26 144 8 128 1.04e-14 67.8
Msa0889310 MtrunA17_Chr3g0132411 35.922 103 61 2 37 139 60 157 1.59e-14 67.0
Msa0889310 MtrunA17_Chr6g0458331 36.082 97 59 2 42 137 37 131 3.63e-14 65.9
Msa0889310 MtrunA17_Chr6g0481491 30.172 116 76 2 24 139 10 120 4.02e-14 66.6
Msa0889310 MtrunA17_Chr4g0041811 29.565 115 78 1 25 139 18 129 5.18e-14 66.6
Msa0889310 MtrunA17_Chr3g0136871 32.000 100 67 1 39 137 24 123 1.14e-12 62.0
Msa0889310 MtrunA17_Chr8g0360371 32.231 121 78 2 23 143 7 123 3.38e-12 62.4
Msa0889310 MtrunA17_Chr4g0062341 31.304 115 75 2 27 139 17 129 6.33e-12 60.1
Msa0889310 MtrunA17_Chr4g0062311 31.304 115 75 2 27 139 17 129 6.33e-12 60.1
Msa0889310 MtrunA17_Chr4g0040291 32.075 106 70 1 39 144 22 125 2.72e-11 58.5
Msa0889310 MtrunA17_Chr2g0324721 32.673 101 66 1 39 139 24 122 6.54e-11 57.8
Msa0889310 MtrunA17_Chr4g0073901 26.400 125 91 1 16 139 3 127 8.49e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0889310 AT2G32300.2 39.855 138 79 1 9 146 23 156 6.07e-28 105
Msa0889310 AT5G20230.1 45.370 108 58 1 40 147 23 129 5.42e-27 100
Msa0889310 AT2G32300.1 41.935 124 68 1 23 146 7 126 2.34e-26 100
Msa0889310 AT2G31050.1 45.000 120 62 2 21 139 7 123 7.64e-25 94.7
Msa0889310 AT5G26330.1 37.600 125 76 1 23 147 5 127 3.95e-22 87.4
Msa0889310 AT2G25060.1 41.322 121 67 2 24 143 16 133 5.90e-22 86.7
Msa0889310 AT3G20570.1 34.646 127 79 2 16 139 1 126 1.59e-21 86.3
Msa0889310 AT3G17675.1 35.593 118 72 1 23 140 13 126 3.62e-21 83.2
Msa0889310 AT2G26720.1 40.000 115 65 3 26 139 12 123 7.29e-21 84.7
Msa0889310 AT3G60270.1 39.815 108 60 2 39 146 23 125 7.65e-20 81.6
Msa0889310 AT4G31840.1 37.815 119 72 2 23 139 7 125 6.16e-18 76.3
Msa0889310 AT1G79800.1 34.146 123 77 3 23 144 16 135 6.03e-17 73.9
Msa0889310 AT1G64640.1 31.452 124 83 2 15 137 5 127 3.31e-16 72.0
Msa0889310 AT5G53870.2 36.364 121 72 2 20 137 5 123 4.94e-15 70.9
Msa0889310 AT5G53870.1 36.364 121 72 2 20 137 5 123 6.57e-15 70.9
Msa0889310 AT2G02850.1 34.483 116 66 3 24 139 21 126 6.71e-15 67.0
Msa0889310 AT5G25090.1 37.895 95 59 0 45 139 30 124 9.35e-15 68.2
Msa0889310 AT5G07475.1 37.255 102 59 2 39 140 27 123 1.23e-14 67.8
Msa0889310 AT1G72230.1 40.000 80 47 1 60 139 37 115 1.90e-14 67.4
Msa0889310 AT1G45063.2 36.364 99 60 2 42 139 149 245 2.56e-14 68.2
Msa0889310 AT1G45063.2 31.897 116 75 3 25 139 12 124 5.66e-12 62.0
Msa0889310 AT4G30590.1 31.092 119 80 2 23 139 8 126 3.25e-14 66.6
Msa0889310 AT4G28365.1 33.884 121 77 3 23 143 11 128 4.69e-14 66.6
Msa0889310 AT1G45063.1 36.364 99 60 2 42 139 149 245 5.10e-14 68.2
Msa0889310 AT1G45063.1 31.897 116 75 3 25 139 12 124 1.03e-11 61.6
Msa0889310 AT2G44790.1 35.398 113 62 4 33 143 23 126 1.14e-13 65.5
Msa0889310 AT3G60280.1 38.614 101 57 2 39 139 21 116 1.74e-13 65.5
Msa0889310 AT4G27520.1 31.624 117 78 1 27 143 16 130 8.01e-13 64.7
Msa0889310 AT2G23990.1 29.565 115 79 2 27 139 12 126 2.46e-12 62.0
Msa0889310 AT1G22480.1 33.058 121 66 3 23 143 10 115 3.42e-12 61.2
Msa0889310 AT4G32490.1 30.392 102 69 1 42 143 31 130 4.91e-12 61.6

Find 35 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTCATATGTACCTTTCC+TGG 0.302641 6_1:-56411092 Msa0889310:CDS
TTGCTACGGATCCATGAAAA+AGG 0.306137 6_1:+56411446 None:intergenic
TTTCCTGGTCACTGTTCTGC+TGG 0.401958 6_1:-56411077 Msa0889310:CDS
TAGACTTCGGATCCCTTGGC+TGG 0.402341 6_1:+56411385 None:intergenic
GATCCGAAGTCTATGCCAAC+TGG 0.420089 6_1:-56411375 Msa0889310:CDS
GGCATAGACTTCGGATCCCT+TGG 0.433725 6_1:+56411381 None:intergenic
TTCGGATCCCTTGGCTGGGA+TGG 0.450055 6_1:+56411390 None:intergenic
ACCATTCATGTTGCTTGCTA+TGG 0.457665 6_1:+56411498 None:intergenic
CTTCACCGTGGGTGATGACA+TGG 0.470735 6_1:-56411419 Msa0889310:CDS
CCAGCGACGGTGACTCTCAC+CGG 0.512034 6_1:-56411128 Msa0889310:CDS
TTGTTCTTACAAGTAATAAG+TGG 0.513067 6_1:+56411532 None:intergenic
GGGCTGGACCATCCCAGCCA+AGG 0.526470 6_1:-56411398 Msa0889310:CDS
ACGGTGACTCTCACCGGAGT+AGG 0.531650 6_1:-56411122 Msa0889310:CDS
TTCACTGTAGGTGACAATCT+TGG 0.533049 6_1:-56411337 Msa0889310:intron
TTCAAAACACGATGTGGCAA+AGG 0.539358 6_1:-56411213 Msa0889310:CDS
TCAGCTTTCGATAGTTGCAA+TGG 0.542896 6_1:-56411182 Msa0889310:CDS
CCGGTGAGAGTCACCGTCGC+TGG 0.543264 6_1:+56411128 None:intergenic
CCGTGGGTGATGACATGGGC+TGG 0.546502 6_1:-56411414 Msa0889310:CDS
CGGTGACTCTCACCGGAGTA+GGG 0.549165 6_1:-56411121 Msa0889310:CDS
GGCTGGACCATCCCAGCCAA+GGG 0.550523 6_1:-56411397 Msa0889310:CDS
GAAATATTGTTTCCCTACTC+CGG 0.551702 6_1:+56411109 None:intergenic
ACCATAGCAAGCAACATGAA+TGG 0.562926 6_1:-56411499 Msa0889310:CDS
TGCCCAGTTGGCATAGACTT+CGG 0.568566 6_1:+56411372 None:intergenic
AGCAGAACAGTGACCAGGAA+AGG 0.575490 6_1:+56411079 None:intergenic
CATGTCATCACCCACGGTGA+AGG 0.580846 6_1:+56411420 None:intergenic
AGACTTCGGATCCCTTGGCT+GGG 0.581146 6_1:+56411386 None:intergenic
CATACTAAAACCTTCACTGT+AGG 0.599735 6_1:-56411349 Msa0889310:CDS
TGCTAATTCAAAACACGATG+TGG 0.601535 6_1:-56411219 Msa0889310:CDS
CCAGCCCATGTCATCACCCA+CGG 0.603422 6_1:+56411414 None:intergenic
CAATGGAACAAACGCACTTG+CGG 0.604289 6_1:-56411165 Msa0889310:CDS
TGACCAGCAGAACAGTGACC+AGG 0.608525 6_1:+56411074 None:intergenic
AAGATTGTCACCTACAGTGA+AGG 0.614101 6_1:+56411339 None:intergenic
ATCCGAAGTCTATGCCAACT+GGG 0.618490 6_1:-56411374 Msa0889310:CDS
CATAACAAACACTCCAGCGA+CGG 0.643148 6_1:-56411141 Msa0889310:CDS
TTCACCGTGGGTGATGACAT+GGG 0.693895 6_1:-56411418 Msa0889310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGTTCTTACAAGTAATAAG+TGG + chr6_1:56411049-56411068 None:intergenic 25.0%
TATTTCATATGTACCTTTCC+TGG - chr6_1:56411486-56411505 Msa0889310:CDS 30.0%
!! TGTTTTTGCGTTGCTTGTTA+TGG - chr6_1:56411105-56411124 Msa0889310:CDS 35.0%
CATACTAAAACCTTCACTGT+AGG - chr6_1:56411229-56411248 Msa0889310:CDS 35.0%
TGCTAATTCAAAACACGATG+TGG - chr6_1:56411359-56411378 Msa0889310:CDS 35.0%
GAAATATTGTTTCCCTACTC+CGG + chr6_1:56411472-56411491 None:intergenic 35.0%
ACCATAGCAAGCAACATGAA+TGG - chr6_1:56411079-56411098 Msa0889310:CDS 40.0%
! ACCATTCATGTTGCTTGCTA+TGG + chr6_1:56411083-56411102 None:intergenic 40.0%
!!! GTTATGGCTAGCCTTTTTCA+TGG - chr6_1:56411121-56411140 Msa0889310:CDS 40.0%
TTGCTACGGATCCATGAAAA+AGG + chr6_1:56411135-56411154 None:intergenic 40.0%
AAGATTGTCACCTACAGTGA+AGG + chr6_1:56411242-56411261 None:intergenic 40.0%
TTCACTGTAGGTGACAATCT+TGG - chr6_1:56411241-56411260 Msa0889310:intron 40.0%
TTCAAAACACGATGTGGCAA+AGG - chr6_1:56411365-56411384 Msa0889310:CDS 40.0%
TCAGCTTTCGATAGTTGCAA+TGG - chr6_1:56411396-56411415 Msa0889310:CDS 40.0%
! CACGGTGAAGGTTTTTGCTA+CGG + chr6_1:56411149-56411168 None:intergenic 45.0%
GTAGCAAAAACCTTCACCGT+GGG - chr6_1:56411148-56411167 Msa0889310:CDS 45.0%
ATCCGAAGTCTATGCCAACT+GGG - chr6_1:56411204-56411223 Msa0889310:CDS 45.0%
!! GGTTTTAGTATGTGCCCAGT+TGG + chr6_1:56411221-56411240 None:intergenic 45.0%
CAATGGAACAAACGCACTTG+CGG - chr6_1:56411413-56411432 Msa0889310:CDS 45.0%
CATAACAAACACTCCAGCGA+CGG - chr6_1:56411437-56411456 Msa0889310:CDS 45.0%
CGTAGCAAAAACCTTCACCG+TGG - chr6_1:56411147-56411166 Msa0889310:CDS 50.0%
TTCACCGTGGGTGATGACAT+GGG - chr6_1:56411160-56411179 Msa0889310:CDS 50.0%
GATCCGAAGTCTATGCCAAC+TGG - chr6_1:56411203-56411222 Msa0889310:CDS 50.0%
TGCCCAGTTGGCATAGACTT+CGG + chr6_1:56411209-56411228 None:intergenic 50.0%
AGCAGAACAGTGACCAGGAA+AGG + chr6_1:56411502-56411521 None:intergenic 50.0%
TTTCCTGGTCACTGTTCTGC+TGG - chr6_1:56411501-56411520 Msa0889310:CDS 50.0%
CATGTCATCACCCACGGTGA+AGG + chr6_1:56411161-56411180 None:intergenic 55.0%
CTTCACCGTGGGTGATGACA+TGG - chr6_1:56411159-56411178 Msa0889310:CDS 55.0%
AGACTTCGGATCCCTTGGCT+GGG + chr6_1:56411195-56411214 None:intergenic 55.0%
TAGACTTCGGATCCCTTGGC+TGG + chr6_1:56411196-56411215 None:intergenic 55.0%
GGCATAGACTTCGGATCCCT+TGG + chr6_1:56411200-56411219 None:intergenic 55.0%
TGACCAGCAGAACAGTGACC+AGG + chr6_1:56411507-56411526 None:intergenic 55.0%
CCAGCCCATGTCATCACCCA+CGG + chr6_1:56411167-56411186 None:intergenic 60.0%
TTCGGATCCCTTGGCTGGGA+TGG + chr6_1:56411191-56411210 None:intergenic 60.0%
ACGGTGACTCTCACCGGAGT+AGG - chr6_1:56411456-56411475 Msa0889310:CDS 60.0%
CGGTGACTCTCACCGGAGTA+GGG - chr6_1:56411457-56411476 Msa0889310:CDS 60.0%
CCGTGGGTGATGACATGGGC+TGG - chr6_1:56411164-56411183 Msa0889310:CDS 65.0%
GGCTGGACCATCCCAGCCAA+GGG - chr6_1:56411181-56411200 Msa0889310:CDS 65.0%
CCAGCGACGGTGACTCTCAC+CGG - chr6_1:56411450-56411469 Msa0889310:CDS 65.0%
GGGCTGGACCATCCCAGCCA+AGG - chr6_1:56411180-56411199 Msa0889310:CDS 70.0%
CCGGTGAGAGTCACCGTCGC+TGG + chr6_1:56411453-56411472 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 56411037 56411563 56411037 ID=Msa0889310;Name=Msa0889310
chr6_1 mRNA 56411037 56411563 56411037 ID=Msa0889310-mRNA-1;Parent=Msa0889310;Name=Msa0889310-mRNA-1;_AED=0.13;_eAED=0.14;_QI=0|0|0|1|1|0.5|2|0|147
chr6_1 exon 56411338 56411563 56411338 ID=Msa0889310-mRNA-1:exon:11143;Parent=Msa0889310-mRNA-1
chr6_1 exon 56411037 56411251 56411037 ID=Msa0889310-mRNA-1:exon:11142;Parent=Msa0889310-mRNA-1
chr6_1 CDS 56411338 56411563 56411338 ID=Msa0889310-mRNA-1:cds;Parent=Msa0889310-mRNA-1
chr6_1 CDS 56411037 56411251 56411037 ID=Msa0889310-mRNA-1:cds;Parent=Msa0889310-mRNA-1
Gene Sequence

>Msa0889310

ATGGCAACTCCACTTATTACTTGTAAGAACAATCACTACACAACCATAGCAAGCAACATGAATGGTCTTGTTTTTGCGTTGCTTGTTATGGCTAGCCTTTTTCATGGATCCGTAGCAAAAACCTTCACCGTGGGTGATGACATGGGCTGGACCATCCCAGCCAAGGGATCCGAAGTCTATGCCAACTGGGCACATACTAAAACCTTCACTGTAGGTGACAATCTTGTGTTCAACTTTGCTAATTCAAAACACGATGTGGCAAAGGTAACAAAATCAGCTTTCGATAGTTGCAATGGAACAAACGCACTTGCGGTCATAACAAACACTCCAGCGACGGTGACTCTCACCGGAGTAGGGAAACAATATTTCATATGTACCTTTCCTGGTCACTGTTCTGCTGGTCAGAAACTGTCGATTGATTACGTTTCTTTAGCATCAACT

Protein sequence

>Msa0889310

MATPLITCKNNHYTTIASNMNGLVFALLVMASLFHGSVAKTFTVGDDMGWTIPAKGSEVYANWAHTKTFTVGDNLVFNFANSKHDVAKVTKSAFDSCNGTNALAVITNTPATVTLTGVGKQYFICTFPGHCSAGQKLSIDYVSLAST