AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0894470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0894470 MtrunA17_Chr1g0169361 90.476 42 3 1 39 79 311 352 1.52e-19 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0894470 AT3G24190.1 85.714 42 5 1 39 79 319 360 2.53e-18 77.8

Find 67 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTTTCTCTCCATTGTT+AGG 0.215554 6_1:-80235441 Msa0894470:five_prime_UTR
GAGAGGGAATTGACGGAGTT+TGG 0.224000 6_1:+80235533 None:intergenic
CAATGGAGAGAAAGGGAAAA+TGG 0.224627 6_1:+80235446 None:intergenic
AATAATTTATATTGTATATA+TGG 0.249228 6_1:-80232281 Msa0894470:three_prime_UTR
AATAATAAATCACCTGATTT+AGG 0.253705 6_1:+80232095 None:intergenic
TGTTCTTAATATTGTGAATC+AGG 0.290660 6_1:+80232375 None:intergenic
GTTACTAGGGTTAAGGAATA+TGG 0.301794 6_1:-80235208 Msa0894470:intron
GCGAGAATCGATGGTGAGTT+TGG 0.305199 6_1:+80235566 None:intergenic
TGGCAGCTCTTTCTTCATTT+CGG 0.324100 6_1:+80232865 None:intergenic
CAACAAATTTGTACTCGAAT+AGG 0.330985 6_1:-80234969 Msa0894470:CDS
CAGGAATTGATTACTTGTAT+AGG 0.331274 6_1:+80232394 None:intergenic
GTATTGGTGGACCTGGATCC+TGG 0.348944 6_1:-80235162 Msa0894470:five_prime_UTR
TTAATATGTTACTAGGGTTA+AGG 0.351314 6_1:-80235215 Msa0894470:intron
AGAGGGAATTGACGGAGTTT+GGG 0.351980 6_1:+80235534 None:intergenic
TCCCTTTCTCTCCATTGTTA+GGG 0.369621 6_1:-80235440 Msa0894470:five_prime_UTR
TACTTTGCGACATGTAGATC+TGG 0.369716 6_1:+80235393 None:intergenic
TTTCAATGTTTGATGCATAA+GGG 0.377123 6_1:-80235047 Msa0894470:exon
TAGGTCTCCATTGATGTGGT+TGG 0.395649 6_1:-80232965 Msa0894470:CDS
TCGATGGTGAGTTTGGGAAT+TGG 0.411483 6_1:+80235573 None:intergenic
TGTCACCTGCTACCTAAATC+AGG 0.412254 6_1:-80232107 Msa0894470:three_prime_UTR
TATGTGAATGAAGGTGAAAA+TGG 0.419960 6_1:-80232899 Msa0894470:CDS
CTCCATTGATGTGGTTGGGT+TGG 0.420334 6_1:-80232960 Msa0894470:CDS
AAATTCAGGAAACTTCCTTA+TGG 0.420915 6_1:+80235128 None:intergenic
CGAGAATCGATGGTGAGTTT+GGG 0.425913 6_1:+80235567 None:intergenic
AGGTCTCCATTGATGTGGTT+GGG 0.427169 6_1:-80232964 Msa0894470:CDS
TTCACAATATTAAGAACATT+AGG 0.430074 6_1:-80232370 Msa0894470:three_prime_UTR
AACCCTAACAATGGAGAGAA+AGG 0.434993 6_1:+80235438 None:intergenic
TTGATGCATAAGGGGATGTT+TGG 0.437646 6_1:-80235038 Msa0894470:CDS
AATGGAGAGAAAGGGAAAAT+GGG 0.441935 6_1:+80235447 None:intergenic
GAGAGATAGGTGAAATGAGA+AGG 0.442996 6_1:+80235623 None:intergenic
TCTGATGAGACAATAAATTC+AGG 0.449478 6_1:+80235114 None:intergenic
TAGGCTGGAGTTTCAATCAA+TGG 0.451150 6_1:-80232351 Msa0894470:three_prime_UTR
ACATGTAGATCTGGGAAGAT+TGG 0.451465 6_1:+80235402 None:intergenic
TAGTACCAGGTCCAGGATCC+AGG 0.480428 6_1:+80235151 None:intergenic
TAGCTAGATTACTAGAAAAG+TGG 0.485781 6_1:+80235073 None:intergenic
AGAGATAGGTGAAATGAGAA+GGG 0.487919 6_1:+80235624 None:intergenic
AGCGGTGAAGCGAGCGAGAA+AGG 0.488084 6_1:+80235491 None:intergenic
TTTGTTGAATATATGCAGAT+AGG 0.489247 6_1:+80234985 None:intergenic
TAAGGAATATGGAATAGAAA+AGG 0.490073 6_1:-80235197 Msa0894470:five_prime_UTR
AACCAACCCAACCACATCAA+TGG 0.491341 6_1:+80232958 None:intergenic
GTGCTGGAAGACAGAGAGAT+AGG 0.498501 6_1:+80235610 None:intergenic
GGTTGGGTTGGTTGACGAGT+GGG 0.507535 6_1:-80232948 Msa0894470:CDS
CAATATTAAGAACATTAGGC+TGG 0.524879 6_1:-80232366 Msa0894470:three_prime_UTR
TGGTGGTGAGAGGGAATTGA+CGG 0.529017 6_1:+80235526 None:intergenic
AATCACCTGATTTAGGTAGC+AGG 0.532267 6_1:+80232102 None:intergenic
TCTTTGGGTATTGGTGGACC+TGG 0.534787 6_1:-80235169 Msa0894470:five_prime_UTR
CTGGACCTGGTACTACCATA+AGG 0.546932 6_1:-80235143 Msa0894470:five_prime_UTR
ACTTTGCGACATGTAGATCT+GGG 0.547807 6_1:+80235394 None:intergenic
TTATCAGGTAAAATAGGCGT+TGG 0.548996 6_1:-80235011 Msa0894470:CDS
GTGGACCTGGATCCTGGACC+TGG 0.552759 6_1:-80235156 Msa0894470:five_prime_UTR
ACCCTAACAATGGAGAGAAA+GGG 0.557073 6_1:+80235439 None:intergenic
TGGTTGGGTTGGTTGACGAG+TGG 0.563824 6_1:-80232949 Msa0894470:CDS
AATTGACGGAGTTTGGGTGA+TGG 0.579938 6_1:+80235540 None:intergenic
GAGAATGAAAACCCTAACAA+TGG 0.582262 6_1:+80235429 None:intergenic
TTCAATGTTTGATGCATAAG+GGG 0.586838 6_1:-80235046 Msa0894470:exon
GTATAAGATTGTTCTGCAGA+AGG 0.590909 6_1:-80234940 Msa0894470:intron
CTTATGGTAGTACCAGGTCC+AGG 0.593749 6_1:+80235144 None:intergenic
AACTTCCTTATGGTAGTACC+AGG 0.594142 6_1:+80235138 None:intergenic
ATGAATCGATGGTGGTGAGA+GGG 0.606923 6_1:+80235517 None:intergenic
GAGAAAGGATGATGAATCGA+TGG 0.612943 6_1:+80235506 None:intergenic
GATGAATCGATGGTGGTGAG+AGG 0.614197 6_1:+80235516 None:intergenic
AATGAAGAAAGAGCTGCCAT+AGG 0.617243 6_1:-80232861 Msa0894470:intron
GAGTTAGACTATGTGAATGA+AGG 0.620549 6_1:-80232908 Msa0894470:CDS
TGATGGTGAGCGAGAATCGA+TGG 0.644413 6_1:+80235557 None:intergenic
ACACTATTTACATATATACA+AGG 0.645479 6_1:+80232155 None:intergenic
CGAAGTGAATCAAGAAACAG+CGG 0.692134 6_1:+80235473 None:intergenic
AAAGGATGATGAATCGATGG+TGG 0.734273 6_1:+80235509 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTATTAATATGTTACT+AGG - chr6_1:80232437-80232456 Msa0894470:intron 10.0%
!! TATTTATTAATATGTTACTA+GGG - chr6_1:80232438-80232457 Msa0894470:intron 10.0%
!! ACATTTATTTAAATAAACAA+AGG + chr6_1:80233646-80233665 None:intergenic 10.0%
!! CTTATATAAAAATAAATTTC+TGG - chr6_1:80234318-80234337 Msa0894470:intron 10.0%
!! ATATTTAATTGATTAAAATG+TGG - chr6_1:80234480-80234499 Msa0894470:intron 10.0%
!! AAAAAAGAAAATTGTATATA+TGG - chr6_1:80235412-80235431 Msa0894470:five_prime_UTR 10.0%
!! GTATATTTATTGAAAATAAC+AGG - chr6_1:80232804-80232823 Msa0894470:intron 15.0%
!!! AAGAATCTTTTCTTATATAT+AGG + chr6_1:80233166-80233185 None:intergenic 15.0%
!! CATTTAATAATTAATGTATC+TGG - chr6_1:80233097-80233116 Msa0894470:intron 15.0%
!!! AAGAATCTTTTCTTATATAT+AGG - chr6_1:80233166-80233185 Msa0894470:intron 15.0%
!!! ATAACTTTAAGTTTATGATA+GGG + chr6_1:80234555-80234574 None:intergenic 15.0%
!!! TATAACTTTAAGTTTATGAT+AGG + chr6_1:80234556-80234575 None:intergenic 15.0%
!! TAAACAATGAAAGAGAAAAA+TGG + chr6_1:80232361-80232380 None:intergenic 20.0%
!!! ATTAGCTAGTTTAAGAAATA+GGG - chr6_1:80232389-80232408 Msa0894470:three_prime_UTR 20.0%
!! AATAGAAAAGGAAAAATCTT+TGG - chr6_1:80232474-80232493 Msa0894470:intron 20.0%
!! ATAGAAAAGGAAAAATCTTT+GGG - chr6_1:80232475-80232494 Msa0894470:intron 20.0%
!!! AAAAGACACATAATATTTTG+TGG + chr6_1:80232767-80232786 None:intergenic 20.0%
!!! TCTTTTCTTATATATAGGAT+CGG + chr6_1:80233062-80233081 None:intergenic 20.0%
!! ACAAATAACTTAATAGAGAT+TGG + chr6_1:80233475-80233494 None:intergenic 20.0%
!!! TAGAAATTGGTTTATCTTAT+TGG + chr6_1:80233970-80233989 None:intergenic 20.0%
!!! TATTTTGACTAGTTAGAAAT+TGG + chr6_1:80233983-80234002 None:intergenic 20.0%
!!! ACTAGTTTAGCTTATTATAA+TGG + chr6_1:80234176-80234195 None:intergenic 20.0%
!!! AAAAATAAATTTCTGGAAGT+TGG - chr6_1:80234325-80234344 Msa0894470:intron 20.0%
!! AAGAAAGAAAATAACATCAT+TGG + chr6_1:80234989-80235008 None:intergenic 20.0%
!! ATTATAAAAGCAAAGAAACA+AGG - chr6_1:80235085-80235104 Msa0894470:five_prime_UTR 20.0%
!!! AGGTTAAGTTTTTTCATAAT+AGG - chr6_1:80235105-80235124 Msa0894470:five_prime_UTR 20.0%
!! ATATAAAAAGGATAACAATG+AGG + chr6_1:80235232-80235251 None:intergenic 20.0%
!! TTCACAATATTAAGAACATT+AGG - chr6_1:80235289-80235308 Msa0894470:intron 20.0%
!! ACACTATTTACATATATACA+AGG + chr6_1:80235451-80235470 None:intergenic 20.0%
!! AATAATAAATCACCTGATTT+AGG + chr6_1:80235567-80235586 None:intergenic 20.0%
!! GTTGAAATTTGAAATGATGA+AGG + chr6_1:80232304-80232323 None:intergenic 25.0%
!! GATTAGCTAGTTTAAGAAAT+AGG - chr6_1:80232388-80232407 Msa0894470:three_prime_UTR 25.0%
! TTAATATGTTACTAGGGTTA+AGG - chr6_1:80232444-80232463 Msa0894470:intron 25.0%
! TAAGGAATATGGAATAGAAA+AGG - chr6_1:80232462-80232481 Msa0894470:intron 25.0%
!! TTTTCAATGTTTGATGCATA+AGG - chr6_1:80232611-80232630 Msa0894470:intron 25.0%
! TTTCAATGTTTGATGCATAA+GGG - chr6_1:80232612-80232631 Msa0894470:intron 25.0%
!!! GGTTTTTTATCAGGTAAAAT+AGG - chr6_1:80232642-80232661 Msa0894470:intron 25.0%
! TTTGTTGAATATATGCAGAT+AGG + chr6_1:80232677-80232696 None:intergenic 25.0%
! ATTGAAAATTGAGACAGTTA+TGG - chr6_1:80232878-80232897 Msa0894470:CDS 25.0%
! TTGAAAATTGAGACAGTTAT+GGG - chr6_1:80232879-80232898 Msa0894470:CDS 25.0%
!! TCTTTTCTTATATATAGGAC+CGG - chr6_1:80233171-80233190 Msa0894470:intron 25.0%
! GTGAATAAAGACATACAAAA+TGG - chr6_1:80233256-80233275 Msa0894470:intron 25.0%
! AGTTGACAATAATGCTTAAA+AGG - chr6_1:80233279-80233298 Msa0894470:intron 25.0%
! GTTGACAATAATGCTTAAAA+GGG - chr6_1:80233280-80233299 Msa0894470:intron 25.0%
!!! AATTTCCTACTTTGTTTTAG+AGG - chr6_1:80233532-80233551 Msa0894470:intron 25.0%
!!! AATTGGACTGCATTTTAATA+AGG - chr6_1:80233618-80233637 Msa0894470:intron 25.0%
! ATGACAAACTATAATACACA+AGG + chr6_1:80233672-80233691 None:intergenic 25.0%
!! GTATTATAGTTTGTCATATG+TGG - chr6_1:80233675-80233694 Msa0894470:intron 25.0%
! ATAAGGCTTATTCGATTTAA+TGG + chr6_1:80234075-80234094 None:intergenic 25.0%
! ATACTAGATGGTAAGTTATA+AGG + chr6_1:80234092-80234111 None:intergenic 25.0%
!! ACTAGTTTAGCTTATACTAA+TGG + chr6_1:80234205-80234224 None:intergenic 25.0%
! CATAAGAAGTAAGAAATGAT+AGG + chr6_1:80234829-80234848 None:intergenic 25.0%
! TAAAGATCTAACAGTTTACA+TGG - chr6_1:80234860-80234879 Msa0894470:intron 25.0%
!! AGATGATCTATTTGATTTCT+TGG - chr6_1:80234915-80234934 Msa0894470:intron 25.0%
!! ATTAGGAATGTTTGAAACAT+GGG + chr6_1:80234942-80234961 None:intergenic 25.0%
!! AATTAGGAATGTTTGAAACA+TGG + chr6_1:80234943-80234962 None:intergenic 25.0%
! ATGTTTCAAACATTCCTAAT+TGG - chr6_1:80234942-80234961 Msa0894470:CDS 25.0%
! TGTTTCAAACATTCCTAATT+GGG - chr6_1:80234943-80234962 Msa0894470:CDS 25.0%
!! ATGATATTTGTCAAGGTTTT+TGG - chr6_1:80235195-80235214 Msa0894470:five_prime_UTR 25.0%
!! GTTATCCTTTTTATATGTAG+AGG - chr6_1:80235236-80235255 Msa0894470:intron 25.0%
! TGTAACCTCTACATATAAAA+AGG + chr6_1:80235244-80235263 None:intergenic 25.0%
! TGTTCTTAATATTGTGAATC+AGG + chr6_1:80235287-80235306 None:intergenic 25.0%
!! TTTGGGAATTGGAGTTTTAA+GGG + chr6_1:80232078-80232097 None:intergenic 30.0%
AAGGAAAAATCTTTGGGTAT+TGG - chr6_1:80232481-80232500 Msa0894470:intron 30.0%
AAATTCAGGAAACTTCCTTA+TGG + chr6_1:80232534-80232553 None:intergenic 30.0%
TCTGATGAGACAATAAATTC+AGG + chr6_1:80232548-80232567 None:intergenic 30.0%
! TATTGTCTCATCAGAGTTTT+TGG - chr6_1:80232553-80232572 Msa0894470:intron 30.0%
! ATTGTCTCATCAGAGTTTTT+GGG - chr6_1:80232554-80232573 Msa0894470:intron 30.0%
TAGCTAGATTACTAGAAAAG+TGG + chr6_1:80232589-80232608 None:intergenic 30.0%
TTCAATGTTTGATGCATAAG+GGG - chr6_1:80232613-80232632 Msa0894470:intron 30.0%
CAACAAATTTGTACTCGAAT+AGG - chr6_1:80232690-80232709 Msa0894470:intron 30.0%
AGAAAAGCTTATTGCCAAAA+TGG - chr6_1:80232980-80232999 Msa0894470:intron 30.0%
TTCTTATATATAGGATCGGA+GGG + chr6_1:80233058-80233077 None:intergenic 30.0%
TTTCTTATATATAGGATCGG+AGG + chr6_1:80233059-80233078 None:intergenic 30.0%
!! CATTTCCTCTAAAACAAAGT+AGG + chr6_1:80233540-80233559 None:intergenic 30.0%
AATGTGTAAACTATCACCAT+AGG + chr6_1:80233586-80233605 None:intergenic 30.0%
ACATTTCCCTATTGTGAAAT+TGG - chr6_1:80233601-80233620 Msa0894470:intron 30.0%
!!! GGTTTGTTGTCTTGTTTTTT+AGG - chr6_1:80233749-80233768 Msa0894470:intron 30.0%
!!! TTGTTGTCTTGTTTTTTAGG+TGG - chr6_1:80233752-80233771 Msa0894470:intron 30.0%
!!! TCTTGTTTTTTAGGTGGTTT+TGG - chr6_1:80233758-80233777 Msa0894470:intron 30.0%
!!! AGTGTTTTGTTTCAATCATG+TGG - chr6_1:80233898-80233917 Msa0894470:intron 30.0%
GCAACTATATCAATACTAGA+TGG + chr6_1:80234104-80234123 None:intergenic 30.0%
! GCTTTTTACCTTACCTTTTT+AGG - chr6_1:80234675-80234694 Msa0894470:intron 30.0%
TATGTGAATGAAGGTGAAAA+TGG - chr6_1:80234760-80234779 Msa0894470:intron 30.0%
GTTTCAAACATTCCTAATTG+GGG - chr6_1:80234944-80234963 Msa0894470:CDS 30.0%
TTTCAAACATTCCTAATTGG+GGG - chr6_1:80234945-80234964 Msa0894470:CDS 30.0%
AAAAAGGATAACAATGAGGA+AGG + chr6_1:80235228-80235247 None:intergenic 30.0%
CAGGAATTGATTACTTGTAT+AGG + chr6_1:80235268-80235287 None:intergenic 30.0%
CAATATTAAGAACATTAGGC+TGG - chr6_1:80235293-80235312 Msa0894470:intron 30.0%
!!! ATTATTTTAGTGCTTTCTGC+TGG - chr6_1:80235581-80235600 Msa0894470:five_prime_UTR 30.0%
! GCTACATCTTTTTCTAACTT+TGG - chr6_1:80235636-80235655 Msa0894470:five_prime_UTR 30.0%
AGAGATAGGTGAAATGAGAA+GGG + chr6_1:80232038-80232057 None:intergenic 35.0%
! GTTTGGGAATTGGAGTTTTA+AGG + chr6_1:80232079-80232098 None:intergenic 35.0%
AATGGAGAGAAAGGGAAAAT+GGG + chr6_1:80232215-80232234 None:intergenic 35.0%
GAGAATGAAAACCCTAACAA+TGG + chr6_1:80232233-80232252 None:intergenic 35.0%
GTTACTAGGGTTAAGGAATA+TGG - chr6_1:80232451-80232470 Msa0894470:intron 35.0%
!! GAAAAATCTTTGGGTATTGG+TGG - chr6_1:80232484-80232503 Msa0894470:intron 35.0%
!!! GGGATGTTTGGTTTTTTATC+AGG - chr6_1:80232633-80232652 Msa0894470:intron 35.0%
TTATCAGGTAAAATAGGCGT+TGG - chr6_1:80232648-80232667 Msa0894470:intron 35.0%
GTATAAGATTGTTCTGCAGA+AGG - chr6_1:80232719-80232738 Msa0894470:intron 35.0%
GCTTGCATAAATACATGTCT+TGG + chr6_1:80233019-80233038 None:intergenic 35.0%
TTTCTTATATATAGGACCGG+AGG - chr6_1:80233174-80233193 Msa0894470:intron 35.0%
TTCTTATATATAGGACCGGA+GGG - chr6_1:80233175-80233194 Msa0894470:intron 35.0%
!!! GTTAGTTTTAGTGCAGTACA+TGG + chr6_1:80233369-80233388 None:intergenic 35.0%
TTTAGAGGAAATGAATGCTC+AGG - chr6_1:80233547-80233566 Msa0894470:intron 35.0%
TGCAGTCCAATTTCACAATA+GGG + chr6_1:80233610-80233629 None:intergenic 35.0%
ATGCAGTCCAATTTCACAAT+AGG + chr6_1:80233611-80233630 None:intergenic 35.0%
!!! TTTTTTAGGTGGTTTTGGCT+GGG - chr6_1:80233763-80233782 Msa0894470:intron 35.0%
TTAAAACCAACACAACAGAG+AGG + chr6_1:80233845-80233864 None:intergenic 35.0%
!! TCAAGAGCATCAACTGTATT+GGG - chr6_1:80233866-80233885 Msa0894470:intron 35.0%
! TCTTTGTTATGTCATTGTGC+AGG - chr6_1:80234132-80234151 Msa0894470:intron 35.0%
CAAAGACGGATATTCAACTA+TGG + chr6_1:80234416-80234435 None:intergenic 35.0%
CATAGTTGAATATCCGTCTT+TGG - chr6_1:80234414-80234433 Msa0894470:intron 35.0%
CAGTTGTTAACAACCAAAGA+CGG + chr6_1:80234430-80234449 None:intergenic 35.0%
GGTTGTTAACAACTGTCTTT+AGG - chr6_1:80234435-80234454 Msa0894470:intron 35.0%
TTAACAACTGTCTTTAGGTG+AGG - chr6_1:80234440-80234459 Msa0894470:intron 35.0%
!! AGGTAAGGTAAAAAGCACTT+GGG + chr6_1:80234671-80234690 None:intergenic 35.0%
!! AAGGTAAGGTAAAAAGCACT+TGG + chr6_1:80234672-80234691 None:intergenic 35.0%
AATGGAGACCTAAAAAGGTA+AGG + chr6_1:80234686-80234705 None:intergenic 35.0%
ACATCAATGGAGACCTAAAA+AGG + chr6_1:80234691-80234710 None:intergenic 35.0%
!!! TTTTTAGGTCTCCATTGATG+TGG - chr6_1:80234690-80234709 Msa0894470:intron 35.0%
GAGTTAGACTATGTGAATGA+AGG - chr6_1:80234751-80234770 Msa0894470:intron 35.0%
ATATCTTATCAGTTGAGAGC+AGG + chr6_1:80234886-80234905 None:intergenic 35.0%
!! AGGATCACATTGCTTTTATG+AGG - chr6_1:80235125-80235144 Msa0894470:five_prime_UTR 35.0%
!! GGATCACATTGCTTTTATGA+GGG - chr6_1:80235126-80235145 Msa0894470:five_prime_UTR 35.0%
GTACATGCAAACAGATCATT+GGG + chr6_1:80235169-80235188 None:intergenic 35.0%
CCTTGACAAATATCATCAAC+AGG + chr6_1:80235191-80235210 None:intergenic 35.0%
!! CCTGTTGATGATATTTGTCA+AGG - chr6_1:80235188-80235207 Msa0894470:five_prime_UTR 35.0%
GAGAGATAGGTGAAATGAGA+AGG + chr6_1:80232039-80232058 None:intergenic 40.0%
AAAGGATGATGAATCGATGG+TGG + chr6_1:80232153-80232172 None:intergenic 40.0%
GAGAAAGGATGATGAATCGA+TGG + chr6_1:80232156-80232175 None:intergenic 40.0%
CGAAGTGAATCAAGAAACAG+CGG + chr6_1:80232189-80232208 None:intergenic 40.0%
CAATGGAGAGAAAGGGAAAA+TGG + chr6_1:80232216-80232235 None:intergenic 40.0%
TTCCCTTTCTCTCCATTGTT+AGG - chr6_1:80232218-80232237 Msa0894470:three_prime_UTR 40.0%
TCCCTTTCTCTCCATTGTTA+GGG - chr6_1:80232219-80232238 Msa0894470:three_prime_UTR 40.0%
ACCCTAACAATGGAGAGAAA+GGG + chr6_1:80232223-80232242 None:intergenic 40.0%
AACCCTAACAATGGAGAGAA+AGG + chr6_1:80232224-80232243 None:intergenic 40.0%
ACATGTAGATCTGGGAAGAT+TGG + chr6_1:80232260-80232279 None:intergenic 40.0%
ACTTTGCGACATGTAGATCT+GGG + chr6_1:80232268-80232287 None:intergenic 40.0%
TACTTTGCGACATGTAGATC+TGG + chr6_1:80232269-80232288 None:intergenic 40.0%
AACTTCCTTATGGTAGTACC+AGG + chr6_1:80232524-80232543 None:intergenic 40.0%
TTGATGCATAAGGGGATGTT+TGG - chr6_1:80232621-80232640 Msa0894470:intron 40.0%
! GCACATTGTCAACACCATTT+TGG + chr6_1:80232997-80233016 None:intergenic 40.0%
TCAATCTTTAGTACTCCCTC+CGG + chr6_1:80233193-80233212 None:intergenic 40.0%
! TTTTAGTGCAGTACATGGTG+TGG + chr6_1:80233364-80233383 None:intergenic 40.0%
! ATGAATGCTCAGGTAGCTTT+TGG - chr6_1:80233557-80233576 Msa0894470:intron 40.0%
!!! AAGGGGTCTGTTTTTGCTTT+TGG - chr6_1:80233720-80233739 Msa0894470:intron 40.0%
!!! AGGGGTCTGTTTTTGCTTTT+GGG - chr6_1:80233721-80233740 Msa0894470:intron 40.0%
!!! GTTTTTTAGGTGGTTTTGGC+TGG - chr6_1:80233762-80233781 Msa0894470:intron 40.0%
!! TCCTGCCTTGTTCTTTGTTT+TGG - chr6_1:80233796-80233815 Msa0894470:intron 40.0%
CCTAGCCAAAACAAAGAACA+AGG + chr6_1:80233804-80233823 None:intergenic 40.0%
!!! CCTTGTTCTTTGTTTTGGCT+AGG - chr6_1:80233801-80233820 Msa0894470:intron 40.0%
!! GTCAAGAGCATCAACTGTAT+TGG - chr6_1:80233865-80233884 Msa0894470:intron 40.0%
TCATTGTGCAGGAGTGATAA+TGG - chr6_1:80234143-80234162 Msa0894470:intron 40.0%
TGGCAGCTCTTTCTTCATTT+CGG + chr6_1:80234797-80234816 None:intergenic 40.0%
AATGAAGAAAGAGCTGCCAT+AGG - chr6_1:80234798-80234817 Msa0894470:intron 40.0%
GAAATGATAGGACTCACCTA+TGG + chr6_1:80234817-80234836 None:intergenic 40.0%
AAACATTCCTAATTGGGGGT+TGG - chr6_1:80234949-80234968 Msa0894470:CDS 40.0%
AACATTCCTAATTGGGGGTT+GGG - chr6_1:80234950-80234969 Msa0894470:CDS 40.0%
GGTACATGCAAACAGATCAT+TGG + chr6_1:80235170-80235189 None:intergenic 40.0%
TAGGCTGGAGTTTCAATCAA+TGG - chr6_1:80235308-80235327 Msa0894470:intron 40.0%
AATCACCTGATTTAGGTAGC+AGG + chr6_1:80235560-80235579 None:intergenic 40.0%
!!! GGAGTTTTAAGGGTTTGTGC+TGG + chr6_1:80232068-80232087 None:intergenic 45.0%
TCGATGGTGAGTTTGGGAAT+TGG + chr6_1:80232089-80232108 None:intergenic 45.0%
CGAGAATCGATGGTGAGTTT+GGG + chr6_1:80232095-80232114 None:intergenic 45.0%
AATTGACGGAGTTTGGGTGA+TGG + chr6_1:80232122-80232141 None:intergenic 45.0%
AGAGGGAATTGACGGAGTTT+GGG + chr6_1:80232128-80232147 None:intergenic 45.0%
! ATGAATCGATGGTGGTGAGA+GGG + chr6_1:80232145-80232164 None:intergenic 45.0%
!!! AGGTAGCTTTTGGAGTCCTA+TGG - chr6_1:80233567-80233586 Msa0894470:intron 45.0%
TTCCATATGTCTGTGCAGCA+AGG - chr6_1:80233701-80233720 Msa0894470:intron 45.0%
TCCATATGTCTGTGCAGCAA+GGG - chr6_1:80233702-80233721 Msa0894470:intron 45.0%
!!! GGGGTCTGTTTTTGCTTTTG+GGG - chr6_1:80233722-80233741 Msa0894470:intron 45.0%
!! TGTTTTTGCTTTTGGGGAGC+TGG - chr6_1:80233728-80233747 Msa0894470:intron 45.0%
GCCAAAACAAAGAACAAGGC+AGG + chr6_1:80233800-80233819 None:intergenic 45.0%
!!! TTGTTTTGGCTAGGTTGCCT+AGG - chr6_1:80233810-80233829 Msa0894470:intron 45.0%
TGTCAAGTGAGATTTCAGCC+TGG - chr6_1:80234349-80234368 Msa0894470:intron 45.0%
TGAGATTTCAGCCTGGATCT+GGG - chr6_1:80234356-80234375 Msa0894470:intron 45.0%
TAGGTCTCCATTGATGTGGT+TGG - chr6_1:80234694-80234713 Msa0894470:intron 45.0%
AGGTCTCCATTGATGTGGTT+GGG - chr6_1:80234695-80234714 Msa0894470:intron 45.0%
AACCAACCCAACCACATCAA+TGG + chr6_1:80234704-80234723 None:intergenic 45.0%
!!! GTGGGCTGCTAGATTTTTTG+AGG - chr6_1:80234729-80234748 Msa0894470:intron 45.0%
TGTCACCTGCTACCTAAATC+AGG - chr6_1:80235552-80235571 Msa0894470:five_prime_UTR 45.0%
!! ATATGTTTATTTAATTAATA+TGG - chr6_1:80234575-80234594 Msa0894470:intron 5.0%
!! AATAATTTATATTGTATATA+TGG - chr6_1:80235378-80235397 Msa0894470:intron 5.0%
GTGCTGGAAGACAGAGAGAT+AGG + chr6_1:80232052-80232071 None:intergenic 50.0%
GCGAGAATCGATGGTGAGTT+TGG + chr6_1:80232096-80232115 None:intergenic 50.0%
! TGATGGTGAGCGAGAATCGA+TGG + chr6_1:80232105-80232124 None:intergenic 50.0%
GAGAGGGAATTGACGGAGTT+TGG + chr6_1:80232129-80232148 None:intergenic 50.0%
! TGGTGGTGAGAGGGAATTGA+CGG + chr6_1:80232136-80232155 None:intergenic 50.0%
! GATGAATCGATGGTGGTGAG+AGG + chr6_1:80232146-80232165 None:intergenic 50.0%
!! TCTTTGGGTATTGGTGGACC+TGG - chr6_1:80232490-80232509 Msa0894470:intron 50.0%
CTTATGGTAGTACCAGGTCC+AGG + chr6_1:80232518-80232537 None:intergenic 50.0%
CTGGACCTGGTACTACCATA+AGG - chr6_1:80232516-80232535 Msa0894470:intron 50.0%
CCATATGTCTGTGCAGCAAG+GGG - chr6_1:80233703-80233722 Msa0894470:intron 50.0%
! CAACACAACAGAGAGGCACA+AGG + chr6_1:80233838-80233857 None:intergenic 50.0%
CTTGTGCCTCTCTGTTGTGT+TGG - chr6_1:80233836-80233855 Msa0894470:intron 50.0%
GTGAGATTTCAGCCTGGATC+TGG - chr6_1:80234355-80234374 Msa0894470:intron 50.0%
! GCCTGGATCTGGGAAGATTT+TGG - chr6_1:80234366-80234385 Msa0894470:intron 50.0%
GCCAAAATCTTCCCAGATCC+AGG + chr6_1:80234370-80234389 None:intergenic 50.0%
CTCCATTGATGTGGTTGGGT+TGG - chr6_1:80234699-80234718 Msa0894470:intron 50.0%
TTAGCACCCAACCCCCAATT+AGG + chr6_1:80234959-80234978 None:intergenic 50.0%
! GTATTGGTGGACCTGGATCC+TGG - chr6_1:80232497-80232516 Msa0894470:intron 55.0%
TAGTACCAGGTCCAGGATCC+AGG + chr6_1:80232511-80232530 None:intergenic 55.0%
CCCCTTGCTGCACAGACATA+TGG + chr6_1:80233706-80233725 None:intergenic 55.0%
!! TGGTTGGGTTGGTTGACGAG+TGG - chr6_1:80234710-80234729 Msa0894470:intron 55.0%
!! GGTTGGGTTGGTTGACGAGT+GGG - chr6_1:80234711-80234730 Msa0894470:intron 55.0%
AGCGGTGAAGCGAGCGAGAA+AGG + chr6_1:80232171-80232190 None:intergenic 60.0%
! CAGAGAGGCACAAGGCACCT+AGG + chr6_1:80233830-80233849 None:intergenic 60.0%
GTGGACCTGGATCCTGGACC+TGG - chr6_1:80232503-80232522 Msa0894470:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 80232022 80235659 80232022 ID=Msa0894470;Name=Msa0894470
chr6_1 mRNA 80232022 80235659 80232022 ID=Msa0894470-mRNA-1;Parent=Msa0894470;Name=Msa0894470-mRNA-1;_AED=0.14;_eAED=0.14;_QI=429|1|1|1|0|0.25|4|404|79
chr6_1 exon 80232022 80232425 80232022 ID=Msa0894470-mRNA-1:exon:13461;Parent=Msa0894470-mRNA-1
chr6_1 exon 80232862 80232984 80232862 ID=Msa0894470-mRNA-1:exon:13460;Parent=Msa0894470-mRNA-1
chr6_1 exon 80234941 80235222 80234941 ID=Msa0894470-mRNA-1:exon:13459;Parent=Msa0894470-mRNA-1
chr6_1 exon 80235396 80235659 80235396 ID=Msa0894470-mRNA-1:exon:13458;Parent=Msa0894470-mRNA-1
chr6_1 five_prime_UTR 80235396 80235659 80235396 ID=Msa0894470-mRNA-1:five_prime_utr;Parent=Msa0894470-mRNA-1
chr6_1 five_prime_UTR 80235058 80235222 80235058 ID=Msa0894470-mRNA-1:five_prime_utr;Parent=Msa0894470-mRNA-1
chr6_1 CDS 80234941 80235057 80234941 ID=Msa0894470-mRNA-1:cds;Parent=Msa0894470-mRNA-1
chr6_1 CDS 80232862 80232984 80232862 ID=Msa0894470-mRNA-1:cds;Parent=Msa0894470-mRNA-1
chr6_1 three_prime_UTR 80232022 80232425 80232022 ID=Msa0894470-mRNA-1:three_prime_utr;Parent=Msa0894470-mRNA-1
Gene Sequence

>Msa0894470

ATGCATAAGGGGATGTTTGGTTTTTTATCAGGTAAAATAGGCGTTGGCATCCTATCTGCATATATTCAACAAATTTGTACTCGAATAGGCGTTGAGTATAAGATTGTTCTGCAGAAGGTCTCCATTGATGTGGTTGGGTTGGTTGACGAGTGGGCTGCTAGATTTTTTGAGGAGTTAGACTATGTGAATGAAGGTGAAAATGGAGATTGTTTTGCCGAAATGAAGAAAGAGCTGCCATAG
Protein sequence

>Msa0894470

MHKGMFGFLSGKIGVGILSAYIQQICTRIGVEYKIVLQKVSIDVVGLVDEWAARFFEELDYVNEGENGDCFAEMKKELP*