Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0732310 | Msa0902410 | 0.815573 | 8.628108e-52 | -8.615850e-47 |
| Msa0851120 | Msa0902410 | 0.802958 | 4.381279e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATGCTCATTGTTGTTAGT+AGG | 0.397626 | 6_1:-100682091 | None:intergenic |
| AGTAGGCTTCATCTCTTGAA+TGG | 0.408840 | 6_1:-100682074 | None:intergenic |
| CAACAATGAGCATGTTACTT+TGG | 0.429504 | 6_1:+100682099 | Msa0902410:CDS |
| GATGATGTCCCTATGTATAA+TGG | 0.449134 | 6_1:+100682498 | Msa0902410:CDS |
| ATGTAGTCAACTTGCAAAAG+TGG | 0.460959 | 6_1:-100682446 | None:intergenic |
| GTAGGCTTCATCTCTTGAAT+GGG | 0.472668 | 6_1:-100682073 | None:intergenic |
| TTCATCTCTTGAATGGGAGG+AGG | 0.490510 | 6_1:-100682067 | None:intergenic |
| GCATGTTACTTTGGAGATGC+AGG | 0.490868 | 6_1:+100682108 | Msa0902410:CDS |
| TCATCTCTTGAATGGGAGGA+GGG | 0.507184 | 6_1:-100682066 | None:intergenic |
| CTGACTATTGCAAATGGATC+AGG | 0.511515 | 6_1:+100682474 | Msa0902410:CDS |
| AGTGGCTGACCATTATACAT+AGG | 0.538075 | 6_1:-100682507 | None:intergenic |
| TTGTGTCTGACTATTGCAAA+TGG | 0.540390 | 6_1:+100682468 | Msa0902410:CDS |
| TTATACTTCTTAATCTCTAG+TGG | 0.543560 | 6_1:-100682525 | None:intergenic |
| GTGGCTGACCATTATACATA+GGG | 0.571454 | 6_1:-100682506 | None:intergenic |
| GGCTTCATCTCTTGAATGGG+AGG | 0.588689 | 6_1:-100682070 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACACTTATGTTGCAATTTAT+GGG | + | chr6_1:100682146-100682165 | Msa0902410:intron | 25.0% |
| !! | TGTTTAAAACCATGACAATA+AGG | + | chr6_1:100682187-100682206 | Msa0902410:intron | 25.0% |
| ! | TGTGTGTTAGATTTAACAAA+AGG | + | chr6_1:100682326-100682345 | Msa0902410:intron | 25.0% |
| ! | GTGTGTTAGATTTAACAAAA+GGG | + | chr6_1:100682327-100682346 | Msa0902410:intron | 25.0% |
| ! | TTATACTTCTTAATCTCTAG+TGG | - | chr6_1:100682528-100682547 | None:intergenic | 25.0% |
| CACACTTATGTTGCAATTTA+TGG | + | chr6_1:100682145-100682164 | Msa0902410:intron | 30.0% | |
| ! | TCTTTTCAACCTTATTGTCA+TGG | - | chr6_1:100682199-100682218 | None:intergenic | 30.0% |
| TAAGGTTGAAAAGAACATCA+TGG | + | chr6_1:100682205-100682224 | Msa0902410:intron | 30.0% | |
| ! | ACATGCTCATTGTTGTTAGT+AGG | - | chr6_1:100682094-100682113 | None:intergenic | 35.0% |
| ! | CAACAATGAGCATGTTACTT+TGG | + | chr6_1:100682099-100682118 | Msa0902410:CDS | 35.0% |
| ! | CTTATGCTGCTACATGTTTT+AGG | + | chr6_1:100682299-100682318 | Msa0902410:intron | 35.0% |
| CATAAATCTAACTCGCACAA+AGG | - | chr6_1:100682376-100682395 | None:intergenic | 35.0% | |
| ATGTAGTCAACTTGCAAAAG+TGG | - | chr6_1:100682449-100682468 | None:intergenic | 35.0% | |
| TTGTGTCTGACTATTGCAAA+TGG | + | chr6_1:100682468-100682487 | Msa0902410:CDS | 35.0% | |
| GATGATGTCCCTATGTATAA+TGG | + | chr6_1:100682498-100682517 | Msa0902410:CDS | 35.0% | |
| GTAGGCTTCATCTCTTGAAT+GGG | - | chr6_1:100682076-100682095 | None:intergenic | 40.0% | |
| AGTAGGCTTCATCTCTTGAA+TGG | - | chr6_1:100682077-100682096 | None:intergenic | 40.0% | |
| ! | ATGGTGGGAAAAGACTCATT+TGG | - | chr6_1:100682420-100682439 | None:intergenic | 40.0% |
| CAAAAGTGGCTACAAATGGT+GGG | - | chr6_1:100682435-100682454 | None:intergenic | 40.0% | |
| CTTGCAAAAGTGGCTACAAA+TGG | - | chr6_1:100682439-100682458 | None:intergenic | 40.0% | |
| CTGACTATTGCAAATGGATC+AGG | + | chr6_1:100682474-100682493 | Msa0902410:CDS | 40.0% | |
| GTGGCTGACCATTATACATA+GGG | - | chr6_1:100682509-100682528 | None:intergenic | 40.0% | |
| AGTGGCTGACCATTATACAT+AGG | - | chr6_1:100682510-100682529 | None:intergenic | 40.0% | |
| TCATCTCTTGAATGGGAGGA+GGG | - | chr6_1:100682069-100682088 | None:intergenic | 45.0% | |
| TTCATCTCTTGAATGGGAGG+AGG | - | chr6_1:100682070-100682089 | None:intergenic | 45.0% | |
| !! | GCATGTTACTTTGGAGATGC+AGG | + | chr6_1:100682108-100682127 | Msa0902410:CDS | 45.0% |
| GCAAAAGTGGCTACAAATGG+TGG | - | chr6_1:100682436-100682455 | None:intergenic | 45.0% | |
| GGCTTCATCTCTTGAATGGG+AGG | - | chr6_1:100682073-100682092 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 100682052 | 100682557 | 100682052 | ID=Msa0902410;Name=Msa0902410 |
| chr6_1 | mRNA | 100682052 | 100682557 | 100682052 | ID=Msa0902410-mRNA-1;Parent=Msa0902410;Name=Msa0902410-mRNA-1;_AED=0.71;_eAED=0.71;_QI=0|0|0|0.5|1|1|2|0|64 |
| chr6_1 | exon | 100682052 | 100682134 | 100682052 | ID=Msa0902410-mRNA-1:exon:17797;Parent=Msa0902410-mRNA-1 |
| chr6_1 | exon | 100682446 | 100682557 | 100682446 | ID=Msa0902410-mRNA-1:exon:17798;Parent=Msa0902410-mRNA-1 |
| chr6_1 | CDS | 100682052 | 100682134 | 100682052 | ID=Msa0902410-mRNA-1:cds;Parent=Msa0902410-mRNA-1 |
| chr6_1 | CDS | 100682446 | 100682557 | 100682446 | ID=Msa0902410-mRNA-1:cds;Parent=Msa0902410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
