AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0903240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0903240 MtrunA17_Chr6g0451441 53.020 149 56 1 1 135 319 467 1.04e-48 162
Msa0903240 MtrunA17_Chr6g0451421 47.826 138 66 2 3 134 9 146 9.15e-39 128
Msa0903240 MtrunA17_Chr2g0325301 44.118 136 70 2 2 131 10 145 4.62e-36 121
Msa0903240 MtrunA17_Chr6g0451411 44.604 139 71 1 1 133 175 313 7.85e-35 122
Msa0903240 MtrunA17_Chr2g0329701 39.516 124 75 0 12 135 250 373 7.44e-25 97.4
Msa0903240 MtrunA17_Chr7g0264421 40.164 122 73 0 12 133 220 341 1.31e-24 96.3
Msa0903240 MtrunA17_Chr6g0451451 45.977 87 47 0 50 136 1 87 7.80e-22 83.2
Msa0903240 MtrunA17_Chr1g0158081 28.333 120 86 0 13 132 1221 1340 5.84e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0903240 AT3G01410.2 44.776 134 68 2 2 129 154 287 1.40e-27 103
Msa0903240 AT3G01410.1 44.776 134 68 2 2 129 154 287 1.40e-27 103
Msa0903240 AT1G24090.1 40.833 120 71 0 12 131 232 351 6.63e-21 86.7
Msa0903240 AT5G51080.3 37.500 120 75 0 12 131 138 257 1.07e-19 82.4
Msa0903240 AT5G51080.1 37.500 120 75 0 12 131 201 320 2.82e-19 82.0
Msa0903240 AT5G51080.2 37.500 120 75 0 12 131 201 320 2.82e-19 82.0

Find 49 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAGCCCCTAAGCTAATTT+TGG 0.153721 6_2:-364239 None:intergenic
GGCATAACCTTGGAAATTTA+AGG 0.184360 6_2:-363727 None:intergenic
CTTTCTTGTACCATTGTATT+TGG 0.185261 6_2:+362245 Msa0903240:CDS
CTTTGCAGTACTATGGATTC+TGG 0.252287 6_2:+362398 Msa0903240:intron
TATGGATTCTGGCAAGGATT+TGG 0.278405 6_2:+362409 Msa0903240:CDS
CTCTGAGTTTGTTGTCAATC+AGG 0.282564 6_2:+362528 Msa0903240:CDS
TATGTTTCTTTGCAGTACTA+TGG 0.304270 6_2:+362391 Msa0903240:intron
ATGGATTCTGGCAAGGATTT+GGG 0.335466 6_2:+362410 Msa0903240:CDS
TTTGAACTTGCCACGACATA+TGG 0.342170 6_2:+364212 Msa0903240:three_prime_UTR
ATGATTTATATTCCAAACTT+TGG 0.371920 6_2:-364174 None:intergenic
GTGTGTGTCGTTGGTCTGCC+AGG 0.395874 6_2:-362875 None:intergenic
GCTTTACTGCTTGCTGATGA+TGG 0.411891 6_2:+362290 Msa0903240:CDS
TGGATGCCCAAAATTAGCTT+AGG 0.436390 6_2:+364232 Msa0903240:three_prime_UTR
CTTTACTGCTTGCTGATGAT+GGG 0.465599 6_2:+362291 Msa0903240:CDS
AAACTCAGAGTCTCCTTTGA+CGG 0.469224 6_2:-362515 None:intergenic
GCTTGCTGATGATGGGAGTC+AGG 0.473864 6_2:+362298 Msa0903240:CDS
AACCTTGGAAATTTAAGGCC+CGG 0.476258 6_2:-363722 None:intergenic
GGATGCCCAAAATTAGCTTA+GGG 0.478567 6_2:+364233 Msa0903240:three_prime_UTR
ACACTTAAGGAGCTTATGTC+TGG 0.479927 6_2:+362894 Msa0903240:CDS
AGACCAACGACACACACTTA+AGG 0.491634 6_2:+362881 Msa0903240:CDS
TCTCCATCCAGCACATTCCT+CGG 0.496932 6_2:+362950 Msa0903240:CDS
TTTAAACACGTTACCGTCAA+AGG 0.509214 6_2:+362502 Msa0903240:CDS
CAGTACTATGGATTCTGGCA+AGG 0.514595 6_2:+362403 Msa0903240:intron
ATGTCGTGGCAAGTTCAAAT+CGG 0.520480 6_2:-364208 None:intergenic
CTCCATCCAGCACATTCCTC+GGG 0.523432 6_2:+362951 Msa0903240:CDS
CAATTACCCGAGGAATGTGC+TGG 0.523567 6_2:-362957 None:intergenic
AGCTCGTTATTATGCAAGCC+GGG 0.525084 6_2:+363704 Msa0903240:CDS
AGTAAAGCTCCTGCACCAGC+TGG 0.540297 6_2:-362275 None:intergenic
GTATTTGGTGCTGAACCAGC+TGG 0.540412 6_2:+362260 Msa0903240:CDS
CAGCTCGTTATTATGCAAGC+CGG 0.540821 6_2:+363703 Msa0903240:CDS
TCCAACAGTTTGGGGAACCC+TGG 0.542372 6_2:+362857 Msa0903240:intron
ATGAGACAGGCGATCATGAA+AGG 0.548157 6_2:+362478 Msa0903240:CDS
AGCCGGGCCTTAAATTTCCA+AGG 0.548554 6_2:+363720 Msa0903240:CDS
GGTGCTGAACCAGCTGGTGC+AGG 0.551244 6_2:+362266 Msa0903240:CDS
CCACCACATTATATCATGAC+TGG 0.554466 6_2:+364319 Msa0903240:three_prime_UTR
GTCTGGAGGCTATGAAGTTG+AGG 0.555604 6_2:+362911 Msa0903240:CDS
GCTCCTTAAGTGTGTGTCGT+TGG 0.557850 6_2:-362884 None:intergenic
TACCCGAGGAATGTGCTGGA+TGG 0.580365 6_2:-362953 None:intergenic
GATGCCCAAAATTAGCTTAG+GGG 0.582782 6_2:+364234 Msa0903240:three_prime_UTR
TGTGTGTCGTTGGTCTGCCA+GGG 0.596653 6_2:-362874 None:intergenic
ATTTGGGCATCAAACAAAAG+AGG 0.600961 6_2:+362426 Msa0903240:CDS
TTTGGGCATCAAACAAAAGA+GGG 0.614208 6_2:+362427 Msa0903240:CDS
TCGCCAGTCATGATATAATG+TGG 0.651159 6_2:-364322 None:intergenic
CTTAAGGAGCTTATGTCTGG+AGG 0.659047 6_2:+362897 Msa0903240:CDS
CCAGTCATGATATAATGTGG+TGG 0.686353 6_2:-364319 None:intergenic
TGGGCATCAAACAAAAGAGG+GGG 0.687228 6_2:+362429 Msa0903240:CDS
TGGTTCAGCACCAAATACAA+TGG 0.687523 6_2:-362255 None:intergenic
ACAATAACATCAAACATCTG+CGG 0.712173 6_2:-364345 None:intergenic
TTGGGCATCAAACAAAAGAG+GGG 0.719635 6_2:+362428 Msa0903240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTAAATATTCATATAATT+AGG - chr6_2:363572-363591 None:intergenic 10.0%
!! ATGTAAATATTCATATAATT+AGG - chr6_2:363572-363591 None:intergenic 10.0%
!!! TTTTTTTTATCTCTTCAATT+TGG + chr6_2:363603-363622 Msa0903240:intron 15.0%
!! ATTAGAAAAAGAAATGTTAT+TGG - chr6_2:363663-363682 None:intergenic 15.0%
!!! ATTTCTTTTTCTAATGTTTA+AGG + chr6_2:363668-363687 Msa0903240:intron 15.0%
!!! TTTCTTTTTCTAATGTTTAA+GGG + chr6_2:363669-363688 Msa0903240:intron 15.0%
!!! TTTTTTTTATCTCTTCAATT+TGG + chr6_2:363603-363622 Msa0903240:intron 15.0%
!! ATTAGAAAAAGAAATGTTAT+TGG - chr6_2:363663-363682 None:intergenic 15.0%
!!! ATTTCTTTTTCTAATGTTTA+AGG + chr6_2:363668-363687 Msa0903240:intron 15.0%
!!! TTTCTTTTTCTAATGTTTAA+GGG + chr6_2:363669-363688 Msa0903240:intron 15.0%
!!! GTAGAAAGTATTTAATTACT+TGG - chr6_2:362669-362688 None:intergenic 20.0%
!!! CTTTGTTATTTTTATAAAGG+GGG - chr6_2:362719-362738 None:intergenic 20.0%
!!! CCTTTGTTATTTTTATAAAG+GGG - chr6_2:362720-362739 None:intergenic 20.0%
!!! GCCTTTGTTATTTTTATAAA+GGG - chr6_2:362721-362740 None:intergenic 20.0%
!!! AGCCTTTGTTATTTTTATAA+AGG - chr6_2:362722-362741 None:intergenic 20.0%
!! AAACAATCTTTACATGAATT+TGG - chr6_2:363110-363129 None:intergenic 20.0%
!! AAACAATCTTTCCATAAATT+TGG + chr6_2:363272-363291 Msa0903240:intron 20.0%
!! TATAGGAATTCTCATTTATT+AGG - chr6_2:363798-363817 None:intergenic 20.0%
!! TTTAAACCAACAAGTATATT+TGG + chr6_2:363837-363856 Msa0903240:intron 20.0%
!! ATTGAAAGTAATATTGTGAA+AGG + chr6_2:363904-363923 Msa0903240:intron 20.0%
!!! GGATATGAAAATAAATTTTG+AGG - chr6_2:363979-363998 None:intergenic 20.0%
!! TTATAATCCTGTATCAAATA+GGG - chr6_2:364000-364019 None:intergenic 20.0%
!! TTTATAATCCTGTATCAAAT+AGG - chr6_2:364001-364020 None:intergenic 20.0%
!! TACAGGATTATAAAATCTAA+TGG + chr6_2:364007-364026 Msa0903240:intron 20.0%
!!! CATTTCTTGTTTTAGTATAT+TGG + chr6_2:364134-364153 Msa0903240:three_prime_UTR 20.0%
!! ATGATTTATATTCCAAACTT+TGG - chr6_2:364177-364196 None:intergenic 20.0%
!!! GTAGAAAGTATTTAATTACT+TGG - chr6_2:362669-362688 None:intergenic 20.0%
!!! CTTTGTTATTTTTATAAAGG+GGG - chr6_2:362719-362738 None:intergenic 20.0%
!!! CCTTTGTTATTTTTATAAAG+GGG - chr6_2:362720-362739 None:intergenic 20.0%
!!! GCCTTTGTTATTTTTATAAA+GGG - chr6_2:362721-362740 None:intergenic 20.0%
!!! AGCCTTTGTTATTTTTATAA+AGG - chr6_2:362722-362741 None:intergenic 20.0%
!! AAACAATCTTTACATGAATT+TGG - chr6_2:363110-363129 None:intergenic 20.0%
!! AAACAATCTTTCCATAAATT+TGG + chr6_2:363272-363291 Msa0903240:intron 20.0%
!! TATAGGAATTCTCATTTATT+AGG - chr6_2:363798-363817 None:intergenic 20.0%
!! TTTAAACCAACAAGTATATT+TGG + chr6_2:363837-363856 Msa0903240:intron 20.0%
!! ATTGAAAGTAATATTGTGAA+AGG + chr6_2:363904-363923 Msa0903240:intron 20.0%
!!! GGATATGAAAATAAATTTTG+AGG - chr6_2:363979-363998 None:intergenic 20.0%
!! TTATAATCCTGTATCAAATA+GGG - chr6_2:364000-364019 None:intergenic 20.0%
!! TTTATAATCCTGTATCAAAT+AGG - chr6_2:364001-364020 None:intergenic 20.0%
!! TACAGGATTATAAAATCTAA+TGG + chr6_2:364007-364026 Msa0903240:intron 20.0%
!!! CATTTCTTGTTTTAGTATAT+TGG + chr6_2:364134-364153 Msa0903240:three_prime_UTR 20.0%
!! ATGATTTATATTCCAAACTT+TGG - chr6_2:364177-364196 None:intergenic 20.0%
! CCCCTTTATAAAAATAACAA+AGG + chr6_2:362717-362736 Msa0903240:intron 25.0%
! TTAACAGTATCTAACCATTT+AGG - chr6_2:362761-362780 None:intergenic 25.0%
! AATTTACTGACACATGTTAT+TGG + chr6_2:362831-362850 Msa0903240:intron 25.0%
! GAGTTCAAAAACAATTACAT+TGG + chr6_2:362987-363006 Msa0903240:intron 25.0%
!! TTTTAAATGCAACCATTTAC+TGG + chr6_2:363039-363058 Msa0903240:intron 25.0%
! ATACATGTCTACCAAATTTA+TGG - chr6_2:363286-363305 None:intergenic 25.0%
! TTTGTTAGGATTCTTATGAT+AGG + chr6_2:363422-363441 Msa0903240:intron 25.0%
!! AACAATATATCTAAGATGCT+GGG - chr6_2:363519-363538 None:intergenic 25.0%
! AAAAGAAATGTTATTGGTAG+AGG - chr6_2:363657-363676 None:intergenic 25.0%
!!! TGTTTGGTTTTCTATATCAA+TGG + chr6_2:363863-363882 Msa0903240:intron 25.0%
! GTAATATTGTGAAAGGTTAA+TGG + chr6_2:363911-363930 Msa0903240:intron 25.0%
!! TTATGTGATACTTTTGTAGA+TGG + chr6_2:364034-364053 Msa0903240:intron 25.0%
!!! TTCTTGTTTTAGTATATTGG+AGG + chr6_2:364137-364156 Msa0903240:three_prime_UTR 25.0%
! ATCTTAAAAACTCTTCTATG+TGG + chr6_2:364293-364312 Msa0903240:three_prime_UTR 25.0%
! CCCCTTTATAAAAATAACAA+AGG + chr6_2:362717-362736 Msa0903240:intron 25.0%
! TTAACAGTATCTAACCATTT+AGG - chr6_2:362761-362780 None:intergenic 25.0%
! AATTTACTGACACATGTTAT+TGG + chr6_2:362831-362850 Msa0903240:intron 25.0%
! GAGTTCAAAAACAATTACAT+TGG + chr6_2:362987-363006 Msa0903240:intron 25.0%
!! TTTTAAATGCAACCATTTAC+TGG + chr6_2:363039-363058 Msa0903240:intron 25.0%
! ATACATGTCTACCAAATTTA+TGG - chr6_2:363286-363305 None:intergenic 25.0%
! TTTGTTAGGATTCTTATGAT+AGG + chr6_2:363422-363441 Msa0903240:intron 25.0%
!! AACAATATATCTAAGATGCT+GGG - chr6_2:363519-363538 None:intergenic 25.0%
! AAAAGAAATGTTATTGGTAG+AGG - chr6_2:363657-363676 None:intergenic 25.0%
!!! TGTTTGGTTTTCTATATCAA+TGG + chr6_2:363863-363882 Msa0903240:intron 25.0%
! GTAATATTGTGAAAGGTTAA+TGG + chr6_2:363911-363930 Msa0903240:intron 25.0%
!! TTATGTGATACTTTTGTAGA+TGG + chr6_2:364034-364053 Msa0903240:intron 25.0%
!!! TTCTTGTTTTAGTATATTGG+AGG + chr6_2:364137-364156 Msa0903240:three_prime_UTR 25.0%
! ATCTTAAAAACTCTTCTATG+TGG + chr6_2:364293-364312 Msa0903240:three_prime_UTR 25.0%
CTTTCTTGTACCATTGTATT+TGG + chr6_2:362245-362264 Msa0903240:CDS 30.0%
ATTCTATGCAACAGTTCATT+TGG + chr6_2:362326-362345 Msa0903240:intron 30.0%
TTCTATGCAACAGTTCATTT+GGG + chr6_2:362327-362346 Msa0903240:intron 30.0%
TATGTTTCTTTGCAGTACTA+TGG + chr6_2:362391-362410 Msa0903240:intron 30.0%
!! ATTGATTTTAGGTATGAGAC+AGG + chr6_2:362465-362484 Msa0903240:CDS 30.0%
ACATTAATAGTAGCAACCTA+AGG + chr6_2:362687-362706 Msa0903240:intron 30.0%
!!! GTTATTTTTATAAAGGGGGT+AGG - chr6_2:362715-362734 None:intergenic 30.0%
TTCAATCCATTGTTCCTAAA+TGG + chr6_2:362744-362763 Msa0903240:intron 30.0%
ATCTAACCATTTAGGAACAA+TGG - chr6_2:362753-362772 None:intergenic 30.0%
CATGTGTCAGTAAATTGTTT+AGG - chr6_2:362827-362846 None:intergenic 30.0%
!!! TTATTGGTTTTCCAACAGTT+TGG + chr6_2:362847-362866 Msa0903240:intron 30.0%
!!! TATTGGTTTTCCAACAGTTT+GGG + chr6_2:362848-362867 Msa0903240:intron 30.0%
CTTAACAAGATACCTATTGA+AGG - chr6_2:363146-363165 None:intergenic 30.0%
CTTCAATAGGTATCTTGTTA+AGG + chr6_2:363144-363163 Msa0903240:intron 30.0%
TAGGTATCTTGTTAAGGAAA+TGG + chr6_2:363150-363169 Msa0903240:intron 30.0%
!! GTAGAAAGTATTGCTAGATT+AGG + chr6_2:363179-363198 Msa0903240:intron 30.0%
AGTAAGTGTATAGCAAACTA+AGG - chr6_2:363217-363236 None:intergenic 30.0%
CTCAAGATATTCAGAAACTT+AGG - chr6_2:363490-363509 None:intergenic 30.0%
! GAACAATATATCTAAGATGC+TGG - chr6_2:363520-363539 None:intergenic 30.0%
AATTAGGATCCTAATGCAAT+TGG - chr6_2:363556-363575 None:intergenic 30.0%
! ACATTTTCTCAGTTCTCTAT+AGG + chr6_2:363755-363774 Msa0903240:intron 30.0%
ACACAACCAAATATACTTGT+TGG - chr6_2:363846-363865 None:intergenic 30.0%
CAAGTATATTTGGTTGTGTT+TGG + chr6_2:363847-363866 Msa0903240:intron 30.0%
!! TTGTGAAAGGTTAATGGATT+TGG + chr6_2:363917-363936 Msa0903240:intron 30.0%
!! AAAGGTTAATGGATTTGGTA+GGG + chr6_2:363922-363941 Msa0903240:intron 30.0%
TTCATATCCCTATTTGATAC+AGG + chr6_2:363990-364009 Msa0903240:intron 30.0%
!!! TTGTTTTAGTATATTGGAGG+AGG + chr6_2:364140-364159 Msa0903240:three_prime_UTR 30.0%
! GTTACAATTTTGCCAAAGTT+TGG + chr6_2:364162-364181 Msa0903240:three_prime_UTR 30.0%
CTTTCTTGTACCATTGTATT+TGG + chr6_2:362245-362264 Msa0903240:CDS 30.0%
ATTCTATGCAACAGTTCATT+TGG + chr6_2:362326-362345 Msa0903240:intron 30.0%
TTCTATGCAACAGTTCATTT+GGG + chr6_2:362327-362346 Msa0903240:intron 30.0%
TATGTTTCTTTGCAGTACTA+TGG + chr6_2:362391-362410 Msa0903240:intron 30.0%
!! ATTGATTTTAGGTATGAGAC+AGG + chr6_2:362465-362484 Msa0903240:CDS 30.0%
ACATTAATAGTAGCAACCTA+AGG + chr6_2:362687-362706 Msa0903240:intron 30.0%
!!! GTTATTTTTATAAAGGGGGT+AGG - chr6_2:362715-362734 None:intergenic 30.0%
TTCAATCCATTGTTCCTAAA+TGG + chr6_2:362744-362763 Msa0903240:intron 30.0%
ATCTAACCATTTAGGAACAA+TGG - chr6_2:362753-362772 None:intergenic 30.0%
CATGTGTCAGTAAATTGTTT+AGG - chr6_2:362827-362846 None:intergenic 30.0%
!!! TTATTGGTTTTCCAACAGTT+TGG + chr6_2:362847-362866 Msa0903240:intron 30.0%
!!! TATTGGTTTTCCAACAGTTT+GGG + chr6_2:362848-362867 Msa0903240:intron 30.0%
CTTAACAAGATACCTATTGA+AGG - chr6_2:363146-363165 None:intergenic 30.0%
CTTCAATAGGTATCTTGTTA+AGG + chr6_2:363144-363163 Msa0903240:intron 30.0%
TAGGTATCTTGTTAAGGAAA+TGG + chr6_2:363150-363169 Msa0903240:intron 30.0%
!! GTAGAAAGTATTGCTAGATT+AGG + chr6_2:363179-363198 Msa0903240:intron 30.0%
AGTAAGTGTATAGCAAACTA+AGG - chr6_2:363217-363236 None:intergenic 30.0%
CTCAAGATATTCAGAAACTT+AGG - chr6_2:363490-363509 None:intergenic 30.0%
! GAACAATATATCTAAGATGC+TGG - chr6_2:363520-363539 None:intergenic 30.0%
AATTAGGATCCTAATGCAAT+TGG - chr6_2:363556-363575 None:intergenic 30.0%
! ACATTTTCTCAGTTCTCTAT+AGG + chr6_2:363755-363774 Msa0903240:intron 30.0%
ACACAACCAAATATACTTGT+TGG - chr6_2:363846-363865 None:intergenic 30.0%
CAAGTATATTTGGTTGTGTT+TGG + chr6_2:363847-363866 Msa0903240:intron 30.0%
!! TTGTGAAAGGTTAATGGATT+TGG + chr6_2:363917-363936 Msa0903240:intron 30.0%
!! AAAGGTTAATGGATTTGGTA+GGG + chr6_2:363922-363941 Msa0903240:intron 30.0%
TTCATATCCCTATTTGATAC+AGG + chr6_2:363990-364009 Msa0903240:intron 30.0%
!!! TTGTTTTAGTATATTGGAGG+AGG + chr6_2:364140-364159 Msa0903240:three_prime_UTR 30.0%
! GTTACAATTTTGCCAAAGTT+TGG + chr6_2:364162-364181 Msa0903240:three_prime_UTR 30.0%
TCTATGCAACAGTTCATTTG+GGG + chr6_2:362328-362347 Msa0903240:intron 35.0%
CTGCAAAGAAACATAAGAGT+TGG - chr6_2:362386-362405 None:intergenic 35.0%
ATTTGGGCATCAAACAAAAG+AGG + chr6_2:362426-362445 Msa0903240:CDS 35.0%
TTTGGGCATCAAACAAAAGA+GGG + chr6_2:362427-362446 Msa0903240:CDS 35.0%
TTTAAACACGTTACCGTCAA+AGG + chr6_2:362502-362521 Msa0903240:CDS 35.0%
!!! ATTGGTTTTCCAACAGTTTG+GGG + chr6_2:362849-362868 Msa0903240:intron 35.0%
CCATGAATAGAACCAGTAAA+TGG - chr6_2:363054-363073 None:intergenic 35.0%
!! CCATTTACTGGTTCTATTCA+TGG + chr6_2:363051-363070 Msa0903240:intron 35.0%
! CATTTTCTGTCACCTTCAAT+AGG + chr6_2:363131-363150 Msa0903240:intron 35.0%
CAGTATATAACTAGCTAGGA+TGG - chr6_2:363331-363350 None:intergenic 35.0%
CGAACAGTATATAACTAGCT+AGG - chr6_2:363335-363354 None:intergenic 35.0%
GATTGTACCAAGCAAATACT+GGG - chr6_2:363383-363402 None:intergenic 35.0%
TGATTGTACCAAGCAAATAC+TGG - chr6_2:363384-363403 None:intergenic 35.0%
GCATTAGCAGTAACTTTGTT+AGG + chr6_2:363408-363427 Msa0903240:intron 35.0%
AATGTTATTGGTAGAGGTTG+AGG - chr6_2:363651-363670 None:intergenic 35.0%
GGCATAACCTTGGAAATTTA+AGG - chr6_2:363730-363749 None:intergenic 35.0%
AGAACTGAGAAAATGTCTTG+AGG - chr6_2:363751-363770 None:intergenic 35.0%
! CAGCTTTGGTTGCAAAATAT+AGG - chr6_2:363815-363834 None:intergenic 35.0%
!! TGTTGGTTTAAAAGCAGCTT+TGG - chr6_2:363829-363848 None:intergenic 35.0%
!! GAAAGGTTAATGGATTTGGT+AGG + chr6_2:363921-363940 Msa0903240:intron 35.0%
!! AAGGTTAATGGATTTGGTAG+GGG + chr6_2:363923-363942 Msa0903240:intron 35.0%
! TTTTGCAAGTGTCATGTTGT+CGG - chr6_2:364095-364114 None:intergenic 35.0%
CATGACACTTGCAAAAACTA+TGG + chr6_2:364099-364118 Msa0903240:three_prime_UTR 35.0%
! TAAGCCCCTAAGCTAATTTT+GGG - chr6_2:364241-364260 None:intergenic 35.0%
! TTAAGCCCCTAAGCTAATTT+TGG - chr6_2:364242-364261 None:intergenic 35.0%
TCTATGCAACAGTTCATTTG+GGG + chr6_2:362328-362347 Msa0903240:intron 35.0%
CTGCAAAGAAACATAAGAGT+TGG - chr6_2:362386-362405 None:intergenic 35.0%
ATTTGGGCATCAAACAAAAG+AGG + chr6_2:362426-362445 Msa0903240:CDS 35.0%
TTTGGGCATCAAACAAAAGA+GGG + chr6_2:362427-362446 Msa0903240:CDS 35.0%
TTTAAACACGTTACCGTCAA+AGG + chr6_2:362502-362521 Msa0903240:CDS 35.0%
!!! ATTGGTTTTCCAACAGTTTG+GGG + chr6_2:362849-362868 Msa0903240:intron 35.0%
CCATGAATAGAACCAGTAAA+TGG - chr6_2:363054-363073 None:intergenic 35.0%
!! CCATTTACTGGTTCTATTCA+TGG + chr6_2:363051-363070 Msa0903240:intron 35.0%
! CATTTTCTGTCACCTTCAAT+AGG + chr6_2:363131-363150 Msa0903240:intron 35.0%
CAGTATATAACTAGCTAGGA+TGG - chr6_2:363331-363350 None:intergenic 35.0%
CGAACAGTATATAACTAGCT+AGG - chr6_2:363335-363354 None:intergenic 35.0%
GATTGTACCAAGCAAATACT+GGG - chr6_2:363383-363402 None:intergenic 35.0%
TGATTGTACCAAGCAAATAC+TGG - chr6_2:363384-363403 None:intergenic 35.0%
GCATTAGCAGTAACTTTGTT+AGG + chr6_2:363408-363427 Msa0903240:intron 35.0%
AATGTTATTGGTAGAGGTTG+AGG - chr6_2:363651-363670 None:intergenic 35.0%
GGCATAACCTTGGAAATTTA+AGG - chr6_2:363730-363749 None:intergenic 35.0%
AGAACTGAGAAAATGTCTTG+AGG - chr6_2:363751-363770 None:intergenic 35.0%
! CAGCTTTGGTTGCAAAATAT+AGG - chr6_2:363815-363834 None:intergenic 35.0%
!! TGTTGGTTTAAAAGCAGCTT+TGG - chr6_2:363829-363848 None:intergenic 35.0%
!! GAAAGGTTAATGGATTTGGT+AGG + chr6_2:363921-363940 Msa0903240:intron 35.0%
!! AAGGTTAATGGATTTGGTAG+GGG + chr6_2:363923-363942 Msa0903240:intron 35.0%
! TTTTGCAAGTGTCATGTTGT+CGG - chr6_2:364095-364114 None:intergenic 35.0%
CATGACACTTGCAAAAACTA+TGG + chr6_2:364099-364118 Msa0903240:three_prime_UTR 35.0%
! TAAGCCCCTAAGCTAATTTT+GGG - chr6_2:364241-364260 None:intergenic 35.0%
! TTAAGCCCCTAAGCTAATTT+TGG - chr6_2:364242-364261 None:intergenic 35.0%
TGGTTCAGCACCAAATACAA+TGG - chr6_2:362258-362277 None:intergenic 40.0%
! CTTTACTGCTTGCTGATGAT+GGG + chr6_2:362291-362310 Msa0903240:CDS 40.0%
CTTTGCAGTACTATGGATTC+TGG + chr6_2:362398-362417 Msa0903240:intron 40.0%
TATGGATTCTGGCAAGGATT+TGG + chr6_2:362409-362428 Msa0903240:CDS 40.0%
ATGGATTCTGGCAAGGATTT+GGG + chr6_2:362410-362429 Msa0903240:CDS 40.0%
TTGGGCATCAAACAAAAGAG+GGG + chr6_2:362428-362447 Msa0903240:CDS 40.0%
!!! CAGTATCGGGCATTGATTTT+AGG + chr6_2:362454-362473 Msa0903240:CDS 40.0%
AAACTCAGAGTCTCCTTTGA+CGG - chr6_2:362518-362537 None:intergenic 40.0%
!! CTCTGAGTTTGTTGTCAATC+AGG + chr6_2:362528-362547 Msa0903240:CDS 40.0%
ACACTTAAGGAGCTTATGTC+TGG + chr6_2:362894-362913 Msa0903240:CDS 40.0%
CACAATTCCCAGTATTTGCT+TGG + chr6_2:363373-363392 Msa0903240:intron 40.0%
TCACTGACACCAATTGCATT+AGG + chr6_2:363544-363563 Msa0903240:intron 40.0%
! AACCTTGGAAATTTAAGGCC+CGG - chr6_2:363725-363744 None:intergenic 40.0%
AATGTCTTGAGGCATAACCT+TGG - chr6_2:363740-363759 None:intergenic 40.0%
ATGTCGTGGCAAGTTCAAAT+CGG - chr6_2:364211-364230 None:intergenic 40.0%
TTTGAACTTGCCACGACATA+TGG + chr6_2:364212-364231 Msa0903240:three_prime_UTR 40.0%
! TGGATGCCCAAAATTAGCTT+AGG + chr6_2:364232-364251 Msa0903240:three_prime_UTR 40.0%
! GGATGCCCAAAATTAGCTTA+GGG + chr6_2:364233-364252 Msa0903240:three_prime_UTR 40.0%
! GATGCCCAAAATTAGCTTAG+GGG + chr6_2:364234-364253 Msa0903240:three_prime_UTR 40.0%
CCAGTCATGATATAATGTGG+TGG - chr6_2:364322-364341 None:intergenic 40.0%
CCACCACATTATATCATGAC+TGG + chr6_2:364319-364338 Msa0903240:three_prime_UTR 40.0%
TCGCCAGTCATGATATAATG+TGG - chr6_2:364325-364344 None:intergenic 40.0%
TGGTTCAGCACCAAATACAA+TGG - chr6_2:362258-362277 None:intergenic 40.0%
! CTTTACTGCTTGCTGATGAT+GGG + chr6_2:362291-362310 Msa0903240:CDS 40.0%
CTTTGCAGTACTATGGATTC+TGG + chr6_2:362398-362417 Msa0903240:intron 40.0%
TATGGATTCTGGCAAGGATT+TGG + chr6_2:362409-362428 Msa0903240:CDS 40.0%
ATGGATTCTGGCAAGGATTT+GGG + chr6_2:362410-362429 Msa0903240:CDS 40.0%
TTGGGCATCAAACAAAAGAG+GGG + chr6_2:362428-362447 Msa0903240:CDS 40.0%
!!! CAGTATCGGGCATTGATTTT+AGG + chr6_2:362454-362473 Msa0903240:CDS 40.0%
AAACTCAGAGTCTCCTTTGA+CGG - chr6_2:362518-362537 None:intergenic 40.0%
!! CTCTGAGTTTGTTGTCAATC+AGG + chr6_2:362528-362547 Msa0903240:CDS 40.0%
ACACTTAAGGAGCTTATGTC+TGG + chr6_2:362894-362913 Msa0903240:CDS 40.0%
CACAATTCCCAGTATTTGCT+TGG + chr6_2:363373-363392 Msa0903240:intron 40.0%
TCACTGACACCAATTGCATT+AGG + chr6_2:363544-363563 Msa0903240:intron 40.0%
! AACCTTGGAAATTTAAGGCC+CGG - chr6_2:363725-363744 None:intergenic 40.0%
AATGTCTTGAGGCATAACCT+TGG - chr6_2:363740-363759 None:intergenic 40.0%
ATGTCGTGGCAAGTTCAAAT+CGG - chr6_2:364211-364230 None:intergenic 40.0%
TTTGAACTTGCCACGACATA+TGG + chr6_2:364212-364231 Msa0903240:three_prime_UTR 40.0%
! TGGATGCCCAAAATTAGCTT+AGG + chr6_2:364232-364251 Msa0903240:three_prime_UTR 40.0%
! GGATGCCCAAAATTAGCTTA+GGG + chr6_2:364233-364252 Msa0903240:three_prime_UTR 40.0%
! GATGCCCAAAATTAGCTTAG+GGG + chr6_2:364234-364253 Msa0903240:three_prime_UTR 40.0%
CCAGTCATGATATAATGTGG+TGG - chr6_2:364322-364341 None:intergenic 40.0%
CCACCACATTATATCATGAC+TGG + chr6_2:364319-364338 Msa0903240:three_prime_UTR 40.0%
TCGCCAGTCATGATATAATG+TGG - chr6_2:364325-364344 None:intergenic 40.0%
! GCTTTACTGCTTGCTGATGA+TGG + chr6_2:362290-362309 Msa0903240:CDS 45.0%
CAGTACTATGGATTCTGGCA+AGG + chr6_2:362403-362422 Msa0903240:intron 45.0%
TGGGCATCAAACAAAAGAGG+GGG + chr6_2:362429-362448 Msa0903240:CDS 45.0%
ATGAGACAGGCGATCATGAA+AGG + chr6_2:362478-362497 Msa0903240:CDS 45.0%
ATAAAGGGGGTAGGTTCCTT+AGG - chr6_2:362706-362725 None:intergenic 45.0%
AGACCAACGACACACACTTA+AGG + chr6_2:362881-362900 Msa0903240:CDS 45.0%
CTTAAGGAGCTTATGTCTGG+AGG + chr6_2:362897-362916 Msa0903240:CDS 45.0%
CTCATCAAAGCAATTACCCG+AGG - chr6_2:362970-362989 None:intergenic 45.0%
CAGCTCGTTATTATGCAAGC+CGG + chr6_2:363703-363722 Msa0903240:CDS 45.0%
AGCTCGTTATTATGCAAGCC+GGG + chr6_2:363704-363723 Msa0903240:CDS 45.0%
! TTTTGGGCATCCATATGTCG+TGG - chr6_2:364225-364244 None:intergenic 45.0%
! GCTTTACTGCTTGCTGATGA+TGG + chr6_2:362290-362309 Msa0903240:CDS 45.0%
CAGTACTATGGATTCTGGCA+AGG + chr6_2:362403-362422 Msa0903240:intron 45.0%
TGGGCATCAAACAAAAGAGG+GGG + chr6_2:362429-362448 Msa0903240:CDS 45.0%
ATGAGACAGGCGATCATGAA+AGG + chr6_2:362478-362497 Msa0903240:CDS 45.0%
ATAAAGGGGGTAGGTTCCTT+AGG - chr6_2:362706-362725 None:intergenic 45.0%
AGACCAACGACACACACTTA+AGG + chr6_2:362881-362900 Msa0903240:CDS 45.0%
CTTAAGGAGCTTATGTCTGG+AGG + chr6_2:362897-362916 Msa0903240:CDS 45.0%
CTCATCAAAGCAATTACCCG+AGG - chr6_2:362970-362989 None:intergenic 45.0%
CAGCTCGTTATTATGCAAGC+CGG + chr6_2:363703-363722 Msa0903240:CDS 45.0%
AGCTCGTTATTATGCAAGCC+GGG + chr6_2:363704-363723 Msa0903240:CDS 45.0%
! TTTTGGGCATCCATATGTCG+TGG - chr6_2:364225-364244 None:intergenic 45.0%
!! GTATTTGGTGCTGAACCAGC+TGG + chr6_2:362260-362279 Msa0903240:CDS 50.0%
GCTCCTTAAGTGTGTGTCGT+TGG - chr6_2:362887-362906 None:intergenic 50.0%
GTCTGGAGGCTATGAAGTTG+AGG + chr6_2:362911-362930 Msa0903240:CDS 50.0%
TCTCCATCCAGCACATTCCT+CGG + chr6_2:362950-362969 Msa0903240:CDS 50.0%
CAATTACCCGAGGAATGTGC+TGG - chr6_2:362960-362979 None:intergenic 50.0%
AGCCGGGCCTTAAATTTCCA+AGG + chr6_2:363720-363739 Msa0903240:CDS 50.0%
!! GTATTTGGTGCTGAACCAGC+TGG + chr6_2:362260-362279 Msa0903240:CDS 50.0%
GCTCCTTAAGTGTGTGTCGT+TGG - chr6_2:362887-362906 None:intergenic 50.0%
GTCTGGAGGCTATGAAGTTG+AGG + chr6_2:362911-362930 Msa0903240:CDS 50.0%
TCTCCATCCAGCACATTCCT+CGG + chr6_2:362950-362969 Msa0903240:CDS 50.0%
CAATTACCCGAGGAATGTGC+TGG - chr6_2:362960-362979 None:intergenic 50.0%
AGCCGGGCCTTAAATTTCCA+AGG + chr6_2:363720-363739 Msa0903240:CDS 50.0%
AGTAAAGCTCCTGCACCAGC+TGG - chr6_2:362278-362297 None:intergenic 55.0%
!! GCTTGCTGATGATGGGAGTC+AGG + chr6_2:362298-362317 Msa0903240:CDS 55.0%
! CAAAAGAGGGGGCGCAGTAT+CGG + chr6_2:362440-362459 Msa0903240:CDS 55.0%
! AAAAGAGGGGGCGCAGTATC+GGG + chr6_2:362441-362460 Msa0903240:CDS 55.0%
TCCAACAGTTTGGGGAACCC+TGG + chr6_2:362857-362876 Msa0903240:intron 55.0%
! TGTGTGTCGTTGGTCTGCCA+GGG - chr6_2:362877-362896 None:intergenic 55.0%
CTCCATCCAGCACATTCCTC+GGG + chr6_2:362951-362970 Msa0903240:CDS 55.0%
TACCCGAGGAATGTGCTGGA+TGG - chr6_2:362956-362975 None:intergenic 55.0%
AGTAAAGCTCCTGCACCAGC+TGG - chr6_2:362278-362297 None:intergenic 55.0%
!! GCTTGCTGATGATGGGAGTC+AGG + chr6_2:362298-362317 Msa0903240:CDS 55.0%
! CAAAAGAGGGGGCGCAGTAT+CGG + chr6_2:362440-362459 Msa0903240:CDS 55.0%
! AAAAGAGGGGGCGCAGTATC+GGG + chr6_2:362441-362460 Msa0903240:CDS 55.0%
TCCAACAGTTTGGGGAACCC+TGG + chr6_2:362857-362876 Msa0903240:intron 55.0%
! TGTGTGTCGTTGGTCTGCCA+GGG - chr6_2:362877-362896 None:intergenic 55.0%
CTCCATCCAGCACATTCCTC+GGG + chr6_2:362951-362970 Msa0903240:CDS 55.0%
TACCCGAGGAATGTGCTGGA+TGG - chr6_2:362956-362975 None:intergenic 55.0%
GCCAGGGTTCCCCAAACTGT+TGG - chr6_2:362861-362880 None:intergenic 60.0%
! GTGTGTGTCGTTGGTCTGCC+AGG - chr6_2:362878-362897 None:intergenic 60.0%
GCCAGGGTTCCCCAAACTGT+TGG - chr6_2:362861-362880 None:intergenic 60.0%
! GTGTGTGTCGTTGGTCTGCC+AGG - chr6_2:362878-362897 None:intergenic 60.0%
! GGTGCTGAACCAGCTGGTGC+AGG + chr6_2:362266-362285 Msa0903240:CDS 65.0%
! GGTGCTGAACCAGCTGGTGC+AGG + chr6_2:362266-362285 Msa0903240:CDS 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 362245 364357 362245 ID=Msa0903240;Name=Msa0903240
chr6_2 mRNA 362245 364357 362245 ID=Msa0903240-mRNA-1;Parent=Msa0903240;Name=Msa0903240-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0.8|1|0.75|0.8|5|273|136
chr6_2 exon 362245 362319 362245 ID=Msa0903240-mRNA-1:exon:168;Parent=Msa0903240-mRNA-1
chr6_2 exon 362406 362549 362406 ID=Msa0903240-mRNA-1:exon:169;Parent=Msa0903240-mRNA-1
chr6_2 exon 362865 362972 362865 ID=Msa0903240-mRNA-1:exon:170;Parent=Msa0903240-mRNA-1
chr6_2 exon 363690 363741 363690 ID=Msa0903240-mRNA-1:exon:171;Parent=Msa0903240-mRNA-1
chr6_2 exon 364053 364357 364053 ID=Msa0903240-mRNA-1:exon:172;Parent=Msa0903240-mRNA-1
chr6_2 CDS 362245 362319 362245 ID=Msa0903240-mRNA-1:cds;Parent=Msa0903240-mRNA-1
chr6_2 CDS 362406 362549 362406 ID=Msa0903240-mRNA-1:cds;Parent=Msa0903240-mRNA-1
chr6_2 CDS 362865 362972 362865 ID=Msa0903240-mRNA-1:cds;Parent=Msa0903240-mRNA-1
chr6_2 CDS 363690 363741 363690 ID=Msa0903240-mRNA-1:cds;Parent=Msa0903240-mRNA-1
chr6_2 CDS 364053 364084 364053 ID=Msa0903240-mRNA-1:cds;Parent=Msa0903240-mRNA-1
chr6_2 three_prime_UTR 364085 364357 364085 ID=Msa0903240-mRNA-1:three_prime_utr;Parent=Msa0903240-mRNA-1
Gene Sequence

>Msa0903240

CTTTCTTGTACCATTGTATTTGGTGCTGAACCAGCTGGTGCAGGAGCTTTACTGCTTGCTGATGATGGGAGTCAGTACTATGGATTCTGGCAAGGATTTGGGCATCAAACAAAAGAGGGGGCGCAGTATCGGGCATTGATTTTAGGTATGAGACAGGCGATCATGAAAGGATTTAAACACGTTACCGTCAAAGGAGACTCTGAGTTTGTTGTCAATCAGTTTGGGGAACCCTGGCAGACCAACGACACACACTTAAGGAGCTTATGTCTGGAGGCTATGAAGTTGAGGAATAGCTTTGACTCATTCTCCATCCAGCACATTCCTCGGGGACAAAACAAAGCAGCTCGTTATTATGCAAGCCGGGCCTTAAATTTCCAAGATGGTGAATATGACGAAGAACGCATCACTTGA
Protein sequence

>Msa0903240

LSCTIVFGAEPAGAGALLLADDGSQYYGFWQGFGHQTKEGAQYRALILGMRQAIMKGFKHVTVKGDSEFVVNQFGEPWQTNDTHLRSLCLEAMKLRNSFDSFSIQHIPRGQNKAARYYASRALNFQDGEYDEERIT*