AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0907020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0907020 MtrunA17_Chr6g0454951 71.795 195 54 1 1 195 957 1150 1.35e-91 290
Msa0907020 MtrunA17_Chr6g0454911 69.231 195 34 2 1 195 98 266 1.67e-89 263
Msa0907020 MtrunA17_Chr6g0455191 74.227 194 34 3 2 195 1189 1366 3.33e-88 282
Msa0907020 MtrunA17_Chr3g0135571 67.598 179 57 1 17 195 1 178 4.79e-82 241
Msa0907020 MtrunA17_Chr6g0455221 68.718 195 49 2 1 195 976 1158 1.98e-81 262
Msa0907020 MtrunA17_Chr3g0135541 67.778 180 57 1 16 195 152 330 3.14e-81 244
Msa0907020 MtrunA17_Chr6g0454971 70.256 195 46 2 1 195 903 1085 3.38e-81 260
Msa0907020 MtrunA17_Chr3g0135511 67.039 179 58 1 17 195 1 178 5.88e-81 238
Msa0907020 MtrunA17_Chr6g0455251 68.718 195 46 3 1 195 988 1167 1.90e-80 259
Msa0907020 MtrunA17_Chr6g0455211 69.753 162 48 1 1 162 905 1065 3.54e-74 241
Msa0907020 MtrunA17_Chr7g0257531 64.497 169 58 2 1 168 520 687 3.75e-68 220
Msa0907020 MtrunA17_Chr6g0454851 66.667 159 52 1 2 160 964 1121 6.20e-67 221
Msa0907020 MtrunA17_Chr4g0014821 62.424 165 60 2 23 187 3 165 5.50e-65 197
Msa0907020 MtrunA17_Chr6g0455131 60.417 192 51 3 1 191 964 1131 2.30e-64 214
Msa0907020 MtrunA17_Chr6g0454961 55.046 218 68 4 1 191 907 1121 1.24e-62 209
Msa0907020 MtrunA17_Chr6g0455551 67.485 163 51 2 1 162 955 1116 4.12e-62 207
Msa0907020 MtrunA17_Chr4g0005851 54.974 191 85 1 1 191 906 1095 8.59e-62 206
Msa0907020 MtrunA17_Chr6g0455031 57.619 210 61 5 1 195 878 1074 1.88e-56 191
Msa0907020 MtrunA17_Chr4g0005591 50.000 190 92 2 2 191 949 1135 2.31e-55 188
Msa0907020 MtrunA17_Chr6g0454831 61.392 158 60 1 1 158 878 1034 2.90e-54 185
Msa0907020 MtrunA17_Chr4g0005871 49.474 190 92 2 2 191 997 1182 3.15e-54 185
Msa0907020 MtrunA17_Chr6g0454821 51.389 216 70 5 1 190 920 1126 3.56e-54 185
Msa0907020 MtrunA17_Chr4g0010711 55.090 167 73 1 1 167 1382 1546 9.97e-54 184
Msa0907020 MtrunA17_Chr6g0454861 66.176 136 20 2 60 195 1 110 4.33e-52 162
Msa0907020 MtrunA17_Chr7g0234901 58.000 150 61 1 1 150 397 544 4.71e-49 167
Msa0907020 MtrunA17_Chr7g0255681 60.417 144 55 1 1 144 1889 2030 6.84e-49 170
Msa0907020 MtrunA17_Chr4g0005891 50.526 190 92 2 2 191 921 1108 7.42e-49 170
Msa0907020 MtrunA17_Chr6g0455141 71.951 82 23 0 114 195 1 82 1.01e-36 122
Msa0907020 MtrunA17_Chr8g0352211 48.889 135 67 2 13 146 803 936 4.93e-35 130
Msa0907020 MtrunA17_Chr4g0009041 46.711 152 77 4 3 153 1374 1522 5.03e-34 127
Msa0907020 MtrunA17_Chr3g0114381 41.497 147 84 2 5 150 100 245 5.76e-34 122
Msa0907020 MtrunA17_Chr4g0009171 44.910 167 88 4 3 167 1590 1754 8.80e-34 127
Msa0907020 MtrunA17_Chr8g0338881 42.361 144 80 3 9 150 899 1041 5.32e-33 124
Msa0907020 MtrunA17_Chr4g0009241 46.309 149 77 3 3 150 1389 1535 3.90e-32 122
Msa0907020 MtrunA17_Chr8g0338931 40.288 139 81 2 13 150 11 148 8.19e-32 116
Msa0907020 MtrunA17_Chr4g0009401 40.860 186 104 5 3 186 1367 1548 2.28e-31 120
Msa0907020 MtrunA17_Chr5g0420341 36.316 190 107 5 5 193 867 1043 3.30e-31 119
Msa0907020 MtrunA17_Chr8g0338981 39.456 147 86 3 5 150 865 1009 8.10e-31 118
Msa0907020 MtrunA17_Chr4g0009301 39.560 182 97 5 4 175 1407 1585 1.09e-30 117
Msa0907020 MtrunA17_Chr5g0420331 39.103 156 90 4 5 159 867 1018 3.23e-30 116
Msa0907020 MtrunA17_Chr4g0009141 42.282 149 83 3 3 150 1385 1531 4.58e-30 116
Msa0907020 MtrunA17_Chr8g0338901 39.456 147 86 3 5 150 888 1032 7.96e-30 115
Msa0907020 MtrunA17_Chr4g0009101 40.964 166 92 4 4 168 1405 1565 8.25e-30 115
Msa0907020 MtrunA17_Chr2g0296451 35.263 190 116 4 5 188 384 572 1.80e-29 114
Msa0907020 MtrunA17_Chr8g0352221 41.216 148 84 3 5 150 781 927 1.90e-29 114
Msa0907020 MtrunA17_Chr4g0009011 40.964 166 92 4 3 167 156 316 5.42e-29 110
Msa0907020 MtrunA17_Chr5g0414761 39.456 147 85 3 5 150 653 796 8.32e-29 112
Msa0907020 MtrunA17_Chr8g0338841 41.667 132 75 2 15 145 319 449 8.55e-29 111
Msa0907020 MtrunA17_Chr8g0370511 36.264 182 102 6 11 183 864 1040 1.85e-28 111
Msa0907020 MtrunA17_Chr6g0454881 60.000 80 31 1 81 160 1 79 2.00e-28 102
Msa0907020 MtrunA17_Chr7g0223531 40.816 147 85 2 5 150 881 1026 3.26e-28 110
Msa0907020 MtrunA17_Chr4g0009211 47.009 117 60 2 52 167 1 116 4.05e-28 102
Msa0907020 MtrunA17_Chr8g0339011 39.073 151 88 4 2 150 857 1005 4.44e-28 110
Msa0907020 MtrunA17_Chr8g0339021 38.562 153 89 4 5 156 856 1004 7.45e-28 109
Msa0907020 MtrunA17_Chr8g0339031 35.811 148 93 2 4 150 871 1017 1.45e-27 108
Msa0907020 MtrunA17_Chr3g0085731 38.776 147 88 2 5 150 873 1018 6.51e-27 107
Msa0907020 MtrunA17_Chr3g0085531 38.776 147 85 3 5 150 66 208 6.08e-26 102
Msa0907020 MtrunA17_Chr8g0338801 39.062 128 73 4 28 153 86 210 3.83e-24 97.1
Msa0907020 MtrunA17_Chr6g0455181 57.692 78 26 2 1 78 250 320 4.85e-23 94.4
Msa0907020 MtrunA17_Chr4g0008981 38.129 139 83 3 52 188 1 138 6.99e-23 89.7
Msa0907020 MtrunA17_Chr4g0009361 40.141 142 81 4 3 141 378 518 1.29e-22 94.7
Msa0907020 MtrunA17_Chr5g0430541 38.168 131 77 3 22 151 392 519 1.61e-20 88.6
Msa0907020 MtrunA17_Chr8g0343781 47.436 78 40 1 60 137 5 81 3.99e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTTTAGATTTATTATTTC+TGG 0.050338 6_2:+5626537 Msa0907020:five_prime_UTR
GAATAAAAGGTTAACCAATT+TGG 0.156402 6_2:-5627230 None:intergenic
AGAAGAACATTCAGAATAAA+AGG 0.229562 6_2:-5627243 None:intergenic
TTCAACTTATAGATACTTCT+TGG 0.270204 6_2:-5626372 None:intergenic
TTGTTTAAATATTGATATAT+TGG 0.286266 6_2:+5626422 Msa0907020:five_prime_UTR
CCGGTGATAGTTATCCAAAT+TGG 0.355985 6_2:+5627216 Msa0907020:CDS
GTGACTGCAAACTTGTTTAT+CGG 0.375374 6_2:+5627771 Msa0907020:three_prime_UTR
TCAAAGGTTTCTCCTACAAT+TGG 0.385254 6_2:+5626291 Msa0907020:five_prime_UTR
TTCAAGTTCCGAGCTGGTAG+TGG 0.388076 6_2:-5626595 None:intergenic
CAAGCAATTGAATTCGAATC+TGG 0.407064 6_2:-5627596 None:intergenic
TCAACTTATAGATACTTCTT+GGG 0.411779 6_2:-5626371 None:intergenic
CTAGAAACTTCAGCCAGAAA+TGG 0.412434 6_2:-5627807 None:intergenic
TGATATATTGGAAGAGGACT+TGG 0.413185 6_2:+5626434 Msa0907020:five_prime_UTR
ACTTTAGATTTATTATTTCT+GGG 0.413861 6_2:+5626538 Msa0907020:five_prime_UTR
GGAAGAGGACTTGGAACAAA+TGG 0.414502 6_2:+5626443 Msa0907020:five_prime_UTR
GATCTACTTAGCAGCAAGAT+TGG 0.430014 6_2:-5627877 None:intergenic
TTTGACCTTGCAACTGAAAA+GGG 0.438981 6_2:-5626513 None:intergenic
TTAAAAGAGACTTCAAGAAA+TGG 0.446177 6_2:-5626694 None:intergenic
TTAGGCAAGCATGAAAAGTC+CGG 0.482068 6_2:-5626231 None:intergenic
TTCTGATGTTATGTTGTCCA+TGG 0.488711 6_2:-5627336 None:intergenic
AAATATTGATATATTGGAAG+AGG 0.493151 6_2:+5626428 Msa0907020:five_prime_UTR
TCAGTAGAGAATCTCTTGAC+AGG 0.493870 6_2:-5627641 None:intergenic
ATTTGTGATGTAGTTGCAGT+TGG 0.494996 6_2:-5626666 None:intergenic
AGTTATGCCCTTCCACTTGA+GGG 0.503447 6_2:-5627280 None:intergenic
GGAGAAACCTTTGATAACAT+TGG 0.513337 6_2:-5626282 None:intergenic
AGCGGTGGTTGCTTCTTCCC+CGG 0.514887 6_2:+5627197 Msa0907020:CDS
CCAATTTGGATAACTATCAC+CGG 0.519080 6_2:-5627216 None:intergenic
TAGGCAAGCATGAAAAGTCC+GGG 0.519706 6_2:-5626230 None:intergenic
TACTTCTTGGGAGGCTACAT+AGG 0.520724 6_2:-5626359 None:intergenic
TTCTGATTGAAGTCATCAGT+GGG 0.523114 6_2:-5627671 None:intergenic
ATTACACTTATAGAGCTGAA+TGG 0.524275 6_2:-5627399 None:intergenic
AAGTTATGCCCTTCCACTTG+AGG 0.525281 6_2:-5627281 None:intergenic
ACGACCTCCACTTTGTTACC+AGG 0.527935 6_2:-5627479 None:intergenic
ACTTGAACTTTCTCATCAGA+TGG 0.530954 6_2:+5626611 Msa0907020:five_prime_UTR
ATTAGTTTCCTTTGAAGATG+AGG 0.532317 6_2:+5627427 Msa0907020:CDS
TTTGAAGTCCCTCAAGTGGA+AGG 0.532396 6_2:+5627272 Msa0907020:CDS
GTTCTGATTGAAGTCATCAG+TGG 0.549628 6_2:-5627672 None:intergenic
AGATTGTCCAATGTTATCAA+AGG 0.554207 6_2:+5626275 Msa0907020:five_prime_UTR
CAAGAGATTCTCTACTGAAG+AGG 0.554484 6_2:+5627646 Msa0907020:CDS
CTGCCCGTCCTCATCTTCAA+AGG 0.557946 6_2:-5627435 None:intergenic
CAATTTGGATAACTATCACC+GGG 0.558896 6_2:-5627215 None:intergenic
CATAGTCTATACTTCAACCA+TGG 0.564030 6_2:+5627319 Msa0907020:CDS
TTGAAGATGAGGACGGGCAG+AGG 0.570651 6_2:+5627438 Msa0907020:CDS
TGTAGTTGCAGTTGGTTGCA+AGG 0.580339 6_2:-5626658 None:intergenic
AGAAAGTTCAAGTTCCGAGC+TGG 0.586660 6_2:-5626601 None:intergenic
GTTTCCTTTGAAGATGAGGA+CGG 0.588818 6_2:+5627431 Msa0907020:CDS
ACTTATAGATACTTCTTGGG+AGG 0.588957 6_2:-5626368 None:intergenic
TCAAGTTCCGAGCTGGTAGT+GGG 0.591128 6_2:-5626594 None:intergenic
GATGAAAGATAGTAAAGCAA+TGG 0.597129 6_2:+5627725 Msa0907020:three_prime_UTR
TTGAAGTCCCTCAAGTGGAA+GGG 0.598230 6_2:+5627273 Msa0907020:CDS
TGTATCTCCCACTACCAGCT+CGG 0.604265 6_2:+5626587 Msa0907020:five_prime_UTR
AAATCTTTCAACAGTCACCA+TGG 0.605119 6_2:-5627837 None:intergenic
CAGTAGAGAATCTCTTGACA+GGG 0.607184 6_2:-5627640 None:intergenic
TTTCCTTTGAAGATGAGGAC+GGG 0.614409 6_2:+5627432 Msa0907020:CDS
TATAGAACCTGGTAACAAAG+TGG 0.620153 6_2:+5627472 Msa0907020:CDS
TCACTGTCTGACAAACACCC+CGG 0.647019 6_2:+5626212 Msa0907020:five_prime_UTR
TTTCATCGATTCATTCCACA+CGG 0.653854 6_2:-5627709 None:intergenic
TTAGTATCAAGTATAGAACC+TGG 0.675876 6_2:+5627461 Msa0907020:CDS
ACACTTATAGAGCTGAATGG+TGG 0.682446 6_2:-5627396 None:intergenic
AGTGTTATTTGCACGCAGAG+TGG 0.684066 6_2:-5626475 None:intergenic
ATGGATGTCAATGTGAGCGG+TGG 0.702164 6_2:+5627182 Msa0907020:CDS
AGAACCTGGTAACAAAGTGG+AGG 0.707895 6_2:+5627475 Msa0907020:CDS
AGGCAAGCATGAAAAGTCCG+GGG 0.710145 6_2:-5626229 None:intergenic
AATATGGATGTCAATGTGAG+CGG 0.747306 6_2:+5627179 Msa0907020:CDS
AATTTGGATAACTATCACCG+GGG 0.761873 6_2:-5627214 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTTAAATATTGATATAT+TGG + chr6_2:5626422-5626441 Msa0907020:five_prime_UTR 10.0%
!!! AAATACTTTTTTTTTATCAA+AGG + chr6_2:5626901-5626920 Msa0907020:intron 10.0%
!! TTGTTTAAATATTGATATAT+TGG + chr6_2:5626422-5626441 Msa0907020:five_prime_UTR 10.0%
!!! AAATACTTTTTTTTTATCAA+AGG + chr6_2:5626901-5626920 Msa0907020:intron 10.0%
!!! AAAAATTCTGTTGATAAATT+TGG + chr6_2:5626323-5626342 Msa0907020:five_prime_UTR 15.0%
!!! ACTTTAGATTTATTATTTCT+GGG + chr6_2:5626538-5626557 Msa0907020:five_prime_UTR 15.0%
!!! AAAAATTCTGTTGATAAATT+TGG + chr6_2:5626323-5626342 Msa0907020:five_prime_UTR 15.0%
!!! ACTTTAGATTTATTATTTCT+GGG + chr6_2:5626538-5626557 Msa0907020:five_prime_UTR 15.0%
!! AAATATTGATATATTGGAAG+AGG + chr6_2:5626428-5626447 Msa0907020:five_prime_UTR 20.0%
!! GACTTTAGATTTATTATTTC+TGG + chr6_2:5626537-5626556 Msa0907020:five_prime_UTR 20.0%
!!! AAGTCTCTTTTAATTCAAAT+TGG + chr6_2:5626704-5626723 Msa0907020:five_prime_UTR 20.0%
!!! TTTGAAAGAGATAATTTTAC+AGG + chr6_2:5626751-5626770 Msa0907020:CDS 20.0%
!! TGTATATGATTATTTGCATT+AGG + chr6_2:5626874-5626893 Msa0907020:intron 20.0%
!! TATTTGTTTAATGAATGACA+AGG + chr6_2:5627108-5627127 Msa0907020:intron 20.0%
!! AAATATTGATATATTGGAAG+AGG + chr6_2:5626428-5626447 Msa0907020:five_prime_UTR 20.0%
!! GACTTTAGATTTATTATTTC+TGG + chr6_2:5626537-5626556 Msa0907020:five_prime_UTR 20.0%
!!! AAGTCTCTTTTAATTCAAAT+TGG + chr6_2:5626704-5626723 Msa0907020:five_prime_UTR 20.0%
!!! TTTGAAAGAGATAATTTTAC+AGG + chr6_2:5626751-5626770 Msa0907020:CDS 20.0%
!! TGTATATGATTATTTGCATT+AGG + chr6_2:5626874-5626893 Msa0907020:intron 20.0%
!! TATTTGTTTAATGAATGACA+AGG + chr6_2:5627108-5627127 Msa0907020:intron 20.0%
! TCAACTTATAGATACTTCTT+GGG - chr6_2:5626374-5626393 None:intergenic 25.0%
! TTCAACTTATAGATACTTCT+TGG - chr6_2:5626375-5626394 None:intergenic 25.0%
! CTAAAAACTTTGATTGTTTC+TGG + chr6_2:5626399-5626418 Msa0907020:five_prime_UTR 25.0%
! TTAAAAGAGACTTCAAGAAA+TGG - chr6_2:5626697-5626716 None:intergenic 25.0%
!! ATCCAAATTCTCACAAATTT+TGG + chr6_2:5626981-5627000 Msa0907020:intron 25.0%
! TACCAAAATTTGTGAGAATT+TGG - chr6_2:5626986-5627005 None:intergenic 25.0%
! GAATAAAAGGTTAACCAATT+TGG - chr6_2:5627233-5627252 None:intergenic 25.0%
! AGAAGAACATTCAGAATAAA+AGG - chr6_2:5627246-5627265 None:intergenic 25.0%
!! TTTTCACTAACAGACATTTA+AGG - chr6_2:5627364-5627383 None:intergenic 25.0%
! TAAACCAGTTTCAAAAACAT+TGG + chr6_2:5627559-5627578 Msa0907020:CDS 25.0%
!!! CATAAGAATTGATTTTTGCT+TGG - chr6_2:5627751-5627770 None:intergenic 25.0%
! CAAGCAAAAATCAATTCTTA+TGG + chr6_2:5627749-5627768 Msa0907020:three_prime_UTR 25.0%
!!! AAGTTTCTAGATTTTATCCA+TGG + chr6_2:5627820-5627839 Msa0907020:three_prime_UTR 25.0%
! TCAACTTATAGATACTTCTT+GGG - chr6_2:5626374-5626393 None:intergenic 25.0%
! TTCAACTTATAGATACTTCT+TGG - chr6_2:5626375-5626394 None:intergenic 25.0%
! CTAAAAACTTTGATTGTTTC+TGG + chr6_2:5626399-5626418 Msa0907020:five_prime_UTR 25.0%
! TTAAAAGAGACTTCAAGAAA+TGG - chr6_2:5626697-5626716 None:intergenic 25.0%
!! ATCCAAATTCTCACAAATTT+TGG + chr6_2:5626981-5627000 Msa0907020:intron 25.0%
! TACCAAAATTTGTGAGAATT+TGG - chr6_2:5626986-5627005 None:intergenic 25.0%
! GAATAAAAGGTTAACCAATT+TGG - chr6_2:5627233-5627252 None:intergenic 25.0%
! AGAAGAACATTCAGAATAAA+AGG - chr6_2:5627246-5627265 None:intergenic 25.0%
!! TTTTCACTAACAGACATTTA+AGG - chr6_2:5627364-5627383 None:intergenic 25.0%
! TAAACCAGTTTCAAAAACAT+TGG + chr6_2:5627559-5627578 Msa0907020:CDS 25.0%
!!! CATAAGAATTGATTTTTGCT+TGG - chr6_2:5627751-5627770 None:intergenic 25.0%
! CAAGCAAAAATCAATTCTTA+TGG + chr6_2:5627749-5627768 Msa0907020:three_prime_UTR 25.0%
!!! AAGTTTCTAGATTTTATCCA+TGG + chr6_2:5627820-5627839 Msa0907020:three_prime_UTR 25.0%
!!! AGTACTAGCTTTTCAAGATT+AGG - chr6_2:5626252-5626271 None:intergenic 30.0%
AGATTGTCCAATGTTATCAA+AGG + chr6_2:5626275-5626294 Msa0907020:five_prime_UTR 30.0%
! TTTTTCTGATGTCCAATTGT+AGG - chr6_2:5626306-5626325 None:intergenic 30.0%
! TCATCAGATGGCATTATTTT+TGG + chr6_2:5626623-5626642 Msa0907020:five_prime_UTR 30.0%
TTAAGAGTTCAAATGTAGGA+GGG - chr6_2:5626825-5626844 None:intergenic 30.0%
ACACTTAAGAGTTCAAATGT+AGG - chr6_2:5626829-5626848 None:intergenic 30.0%
TTGTTTAATGAATGACAAGG+TGG + chr6_2:5627111-5627130 Msa0907020:intron 30.0%
ATCTCTACTTTCACAGAATA+TGG + chr6_2:5627163-5627182 Msa0907020:intron 30.0%
GTTAGTGAAAAATTACACGA+AGG + chr6_2:5627373-5627392 Msa0907020:CDS 30.0%
ATTACACTTATAGAGCTGAA+TGG - chr6_2:5627402-5627421 None:intergenic 30.0%
! ATTAGTTTCCTTTGAAGATG+AGG + chr6_2:5627427-5627446 Msa0907020:CDS 30.0%
TTAGTATCAAGTATAGAACC+TGG + chr6_2:5627461-5627480 Msa0907020:CDS 30.0%
!!! AACTCCAATGTTTTTGAAAC+TGG - chr6_2:5627566-5627585 None:intergenic 30.0%
GATGAAAGATAGTAAAGCAA+TGG + chr6_2:5627725-5627744 Msa0907020:three_prime_UTR 30.0%
! CAACAATTGTTTTCCATTTC+TGG + chr6_2:5627794-5627813 Msa0907020:three_prime_UTR 30.0%
!!! AGTACTAGCTTTTCAAGATT+AGG - chr6_2:5626252-5626271 None:intergenic 30.0%
AGATTGTCCAATGTTATCAA+AGG + chr6_2:5626275-5626294 Msa0907020:five_prime_UTR 30.0%
! TTTTTCTGATGTCCAATTGT+AGG - chr6_2:5626306-5626325 None:intergenic 30.0%
! TCATCAGATGGCATTATTTT+TGG + chr6_2:5626623-5626642 Msa0907020:five_prime_UTR 30.0%
TTAAGAGTTCAAATGTAGGA+GGG - chr6_2:5626825-5626844 None:intergenic 30.0%
ACACTTAAGAGTTCAAATGT+AGG - chr6_2:5626829-5626848 None:intergenic 30.0%
TTGTTTAATGAATGACAAGG+TGG + chr6_2:5627111-5627130 Msa0907020:intron 30.0%
ATCTCTACTTTCACAGAATA+TGG + chr6_2:5627163-5627182 Msa0907020:intron 30.0%
GTTAGTGAAAAATTACACGA+AGG + chr6_2:5627373-5627392 Msa0907020:CDS 30.0%
ATTACACTTATAGAGCTGAA+TGG - chr6_2:5627402-5627421 None:intergenic 30.0%
! ATTAGTTTCCTTTGAAGATG+AGG + chr6_2:5627427-5627446 Msa0907020:CDS 30.0%
TTAGTATCAAGTATAGAACC+TGG + chr6_2:5627461-5627480 Msa0907020:CDS 30.0%
!!! AACTCCAATGTTTTTGAAAC+TGG - chr6_2:5627566-5627585 None:intergenic 30.0%
GATGAAAGATAGTAAAGCAA+TGG + chr6_2:5627725-5627744 Msa0907020:three_prime_UTR 30.0%
! CAACAATTGTTTTCCATTTC+TGG + chr6_2:5627794-5627813 Msa0907020:three_prime_UTR 30.0%
! GGAGAAACCTTTGATAACAT+TGG - chr6_2:5626285-5626304 None:intergenic 35.0%
TCAAAGGTTTCTCCTACAAT+TGG + chr6_2:5626291-5626310 Msa0907020:five_prime_UTR 35.0%
ACTTATAGATACTTCTTGGG+AGG - chr6_2:5626371-5626390 None:intergenic 35.0%
TGATATATTGGAAGAGGACT+TGG + chr6_2:5626434-5626453 Msa0907020:five_prime_UTR 35.0%
TTTGACCTTGCAACTGAAAA+GGG - chr6_2:5626516-5626535 None:intergenic 35.0%
! TTTTGACCTTGCAACTGAAA+AGG - chr6_2:5626517-5626536 None:intergenic 35.0%
ACTTGAACTTTCTCATCAGA+TGG + chr6_2:5626611-5626630 Msa0907020:five_prime_UTR 35.0%
ATTTGTGATGTAGTTGCAGT+TGG - chr6_2:5626669-5626688 None:intergenic 35.0%
ATACACACACAAACATTTGG+GGG - chr6_2:5626801-5626820 None:intergenic 35.0%
GATACACACACAAACATTTG+GGG - chr6_2:5626802-5626821 None:intergenic 35.0%
GGATACACACACAAACATTT+GGG - chr6_2:5626803-5626822 None:intergenic 35.0%
TAAGAGTTCAAATGTAGGAG+GGG - chr6_2:5626824-5626843 None:intergenic 35.0%
CTTAAGAGTTCAAATGTAGG+AGG - chr6_2:5626826-5626845 None:intergenic 35.0%
AATATGGATGTCAATGTGAG+CGG + chr6_2:5627179-5627198 Msa0907020:CDS 35.0%
AATTTGGATAACTATCACCG+GGG - chr6_2:5627217-5627236 None:intergenic 35.0%
CAATTTGGATAACTATCACC+GGG - chr6_2:5627218-5627237 None:intergenic 35.0%
CCAATTTGGATAACTATCAC+CGG - chr6_2:5627219-5627238 None:intergenic 35.0%
CATAGTCTATACTTCAACCA+TGG + chr6_2:5627319-5627338 Msa0907020:CDS 35.0%
TTCTGATGTTATGTTGTCCA+TGG - chr6_2:5627339-5627358 None:intergenic 35.0%
TATAGAACCTGGTAACAAAG+TGG + chr6_2:5627472-5627491 Msa0907020:CDS 35.0%
CAAGCAATTGAATTCGAATC+TGG - chr6_2:5627599-5627618 None:intergenic 35.0%
TTCTGATTGAAGTCATCAGT+GGG - chr6_2:5627674-5627693 None:intergenic 35.0%
!! TTTCATCGATTCATTCCACA+CGG - chr6_2:5627712-5627731 None:intergenic 35.0%
! GTGACTGCAAACTTGTTTAT+CGG + chr6_2:5627771-5627790 Msa0907020:three_prime_UTR 35.0%
AAATCTTTCAACAGTCACCA+TGG - chr6_2:5627840-5627859 None:intergenic 35.0%
! GGAGAAACCTTTGATAACAT+TGG - chr6_2:5626285-5626304 None:intergenic 35.0%
TCAAAGGTTTCTCCTACAAT+TGG + chr6_2:5626291-5626310 Msa0907020:five_prime_UTR 35.0%
ACTTATAGATACTTCTTGGG+AGG - chr6_2:5626371-5626390 None:intergenic 35.0%
TGATATATTGGAAGAGGACT+TGG + chr6_2:5626434-5626453 Msa0907020:five_prime_UTR 35.0%
TTTGACCTTGCAACTGAAAA+GGG - chr6_2:5626516-5626535 None:intergenic 35.0%
! TTTTGACCTTGCAACTGAAA+AGG - chr6_2:5626517-5626536 None:intergenic 35.0%
ACTTGAACTTTCTCATCAGA+TGG + chr6_2:5626611-5626630 Msa0907020:five_prime_UTR 35.0%
ATTTGTGATGTAGTTGCAGT+TGG - chr6_2:5626669-5626688 None:intergenic 35.0%
ATACACACACAAACATTTGG+GGG - chr6_2:5626801-5626820 None:intergenic 35.0%
GATACACACACAAACATTTG+GGG - chr6_2:5626802-5626821 None:intergenic 35.0%
GGATACACACACAAACATTT+GGG - chr6_2:5626803-5626822 None:intergenic 35.0%
TAAGAGTTCAAATGTAGGAG+GGG - chr6_2:5626824-5626843 None:intergenic 35.0%
CTTAAGAGTTCAAATGTAGG+AGG - chr6_2:5626826-5626845 None:intergenic 35.0%
AATATGGATGTCAATGTGAG+CGG + chr6_2:5627179-5627198 Msa0907020:CDS 35.0%
AATTTGGATAACTATCACCG+GGG - chr6_2:5627217-5627236 None:intergenic 35.0%
CAATTTGGATAACTATCACC+GGG - chr6_2:5627218-5627237 None:intergenic 35.0%
CCAATTTGGATAACTATCAC+CGG - chr6_2:5627219-5627238 None:intergenic 35.0%
CATAGTCTATACTTCAACCA+TGG + chr6_2:5627319-5627338 Msa0907020:CDS 35.0%
TTCTGATGTTATGTTGTCCA+TGG - chr6_2:5627339-5627358 None:intergenic 35.0%
TATAGAACCTGGTAACAAAG+TGG + chr6_2:5627472-5627491 Msa0907020:CDS 35.0%
CAAGCAATTGAATTCGAATC+TGG - chr6_2:5627599-5627618 None:intergenic 35.0%
TTCTGATTGAAGTCATCAGT+GGG - chr6_2:5627674-5627693 None:intergenic 35.0%
!! TTTCATCGATTCATTCCACA+CGG - chr6_2:5627712-5627731 None:intergenic 35.0%
! GTGACTGCAAACTTGTTTAT+CGG + chr6_2:5627771-5627790 Msa0907020:three_prime_UTR 35.0%
AAATCTTTCAACAGTCACCA+TGG - chr6_2:5627840-5627859 None:intergenic 35.0%
TTAGGCAAGCATGAAAAGTC+CGG - chr6_2:5626234-5626253 None:intergenic 40.0%
TACACACACAAACATTTGGG+GGG - chr6_2:5626800-5626819 None:intergenic 40.0%
GGGATACACACACAAACATT+TGG - chr6_2:5626804-5626823 None:intergenic 40.0%
CCGGTGATAGTTATCCAAAT+TGG + chr6_2:5627216-5627235 Msa0907020:CDS 40.0%
!!! AACTTTTGAAGTCCCTCAAG+TGG + chr6_2:5627268-5627287 Msa0907020:CDS 40.0%
ACACTTATAGAGCTGAATGG+TGG - chr6_2:5627399-5627418 None:intergenic 40.0%
! GTTTCCTTTGAAGATGAGGA+CGG + chr6_2:5627431-5627450 Msa0907020:CDS 40.0%
! TTTCCTTTGAAGATGAGGAC+GGG + chr6_2:5627432-5627451 Msa0907020:CDS 40.0%
CAGTAGAGAATCTCTTGACA+GGG - chr6_2:5627643-5627662 None:intergenic 40.0%
TCAGTAGAGAATCTCTTGAC+AGG - chr6_2:5627644-5627663 None:intergenic 40.0%
CAAGAGATTCTCTACTGAAG+AGG + chr6_2:5627646-5627665 Msa0907020:CDS 40.0%
GTTCTGATTGAAGTCATCAG+TGG - chr6_2:5627675-5627694 None:intergenic 40.0%
CAGAAAAAAGAAAAGCCGTG+TGG + chr6_2:5627694-5627713 Msa0907020:CDS 40.0%
CTAGAAACTTCAGCCAGAAA+TGG - chr6_2:5627810-5627829 None:intergenic 40.0%
GATCTACTTAGCAGCAAGAT+TGG - chr6_2:5627880-5627899 None:intergenic 40.0%
TTAGGCAAGCATGAAAAGTC+CGG - chr6_2:5626234-5626253 None:intergenic 40.0%
TACACACACAAACATTTGGG+GGG - chr6_2:5626800-5626819 None:intergenic 40.0%
GGGATACACACACAAACATT+TGG - chr6_2:5626804-5626823 None:intergenic 40.0%
CCGGTGATAGTTATCCAAAT+TGG + chr6_2:5627216-5627235 Msa0907020:CDS 40.0%
!!! AACTTTTGAAGTCCCTCAAG+TGG + chr6_2:5627268-5627287 Msa0907020:CDS 40.0%
ACACTTATAGAGCTGAATGG+TGG - chr6_2:5627399-5627418 None:intergenic 40.0%
! GTTTCCTTTGAAGATGAGGA+CGG + chr6_2:5627431-5627450 Msa0907020:CDS 40.0%
! TTTCCTTTGAAGATGAGGAC+GGG + chr6_2:5627432-5627451 Msa0907020:CDS 40.0%
CAGTAGAGAATCTCTTGACA+GGG - chr6_2:5627643-5627662 None:intergenic 40.0%
TCAGTAGAGAATCTCTTGAC+AGG - chr6_2:5627644-5627663 None:intergenic 40.0%
CAAGAGATTCTCTACTGAAG+AGG + chr6_2:5627646-5627665 Msa0907020:CDS 40.0%
GTTCTGATTGAAGTCATCAG+TGG - chr6_2:5627675-5627694 None:intergenic 40.0%
CAGAAAAAAGAAAAGCCGTG+TGG + chr6_2:5627694-5627713 Msa0907020:CDS 40.0%
CTAGAAACTTCAGCCAGAAA+TGG - chr6_2:5627810-5627829 None:intergenic 40.0%
GATCTACTTAGCAGCAAGAT+TGG - chr6_2:5627880-5627899 None:intergenic 40.0%
TAGGCAAGCATGAAAAGTCC+GGG - chr6_2:5626233-5626252 None:intergenic 45.0%
TACTTCTTGGGAGGCTACAT+AGG - chr6_2:5626362-5626381 None:intergenic 45.0%
GGAAGAGGACTTGGAACAAA+TGG + chr6_2:5626443-5626462 Msa0907020:five_prime_UTR 45.0%
!! AGTGTTATTTGCACGCAGAG+TGG - chr6_2:5626478-5626497 None:intergenic 45.0%
! GAGTACCCTTTTCAGTTGCA+AGG + chr6_2:5626508-5626527 Msa0907020:five_prime_UTR 45.0%
! AGAAAGTTCAAGTTCCGAGC+TGG - chr6_2:5626604-5626623 None:intergenic 45.0%
TGTAGTTGCAGTTGGTTGCA+AGG - chr6_2:5626661-5626680 None:intergenic 45.0%
CTGACAGCTTACTTGATACG+TGG + chr6_2:5627013-5627032 Msa0907020:intron 45.0%
! TTTGAAGTCCCTCAAGTGGA+AGG + chr6_2:5627272-5627291 Msa0907020:CDS 45.0%
TTGAAGTCCCTCAAGTGGAA+GGG + chr6_2:5627273-5627292 Msa0907020:CDS 45.0%
AGTTATGCCCTTCCACTTGA+GGG - chr6_2:5627283-5627302 None:intergenic 45.0%
AAGTTATGCCCTTCCACTTG+AGG - chr6_2:5627284-5627303 None:intergenic 45.0%
AGAACCTGGTAACAAAGTGG+AGG + chr6_2:5627475-5627494 Msa0907020:CDS 45.0%
TAGGCAAGCATGAAAAGTCC+GGG - chr6_2:5626233-5626252 None:intergenic 45.0%
TACTTCTTGGGAGGCTACAT+AGG - chr6_2:5626362-5626381 None:intergenic 45.0%
GGAAGAGGACTTGGAACAAA+TGG + chr6_2:5626443-5626462 Msa0907020:five_prime_UTR 45.0%
!! AGTGTTATTTGCACGCAGAG+TGG - chr6_2:5626478-5626497 None:intergenic 45.0%
! GAGTACCCTTTTCAGTTGCA+AGG + chr6_2:5626508-5626527 Msa0907020:five_prime_UTR 45.0%
! AGAAAGTTCAAGTTCCGAGC+TGG - chr6_2:5626604-5626623 None:intergenic 45.0%
TGTAGTTGCAGTTGGTTGCA+AGG - chr6_2:5626661-5626680 None:intergenic 45.0%
CTGACAGCTTACTTGATACG+TGG + chr6_2:5627013-5627032 Msa0907020:intron 45.0%
! TTTGAAGTCCCTCAAGTGGA+AGG + chr6_2:5627272-5627291 Msa0907020:CDS 45.0%
TTGAAGTCCCTCAAGTGGAA+GGG + chr6_2:5627273-5627292 Msa0907020:CDS 45.0%
AGTTATGCCCTTCCACTTGA+GGG - chr6_2:5627283-5627302 None:intergenic 45.0%
AAGTTATGCCCTTCCACTTG+AGG - chr6_2:5627284-5627303 None:intergenic 45.0%
AGAACCTGGTAACAAAGTGG+AGG + chr6_2:5627475-5627494 Msa0907020:CDS 45.0%
!! ATTTAAAAATAAATATGTTA+AGG - chr6_2:5626957-5626976 None:intergenic 5.0%
!! ATTTAAAAATAAATATGTTA+AGG - chr6_2:5626957-5626976 None:intergenic 5.0%
TCACTGTCTGACAAACACCC+CGG + chr6_2:5626212-5626231 Msa0907020:five_prime_UTR 50.0%
AGGCAAGCATGAAAAGTCCG+GGG - chr6_2:5626232-5626251 None:intergenic 50.0%
TGTATCTCCCACTACCAGCT+CGG + chr6_2:5626587-5626606 Msa0907020:five_prime_UTR 50.0%
TCAAGTTCCGAGCTGGTAGT+GGG - chr6_2:5626597-5626616 None:intergenic 50.0%
! TTCAAGTTCCGAGCTGGTAG+TGG - chr6_2:5626598-5626617 None:intergenic 50.0%
ACACACAAACATTTGGGGGG+CGG - chr6_2:5626797-5626816 None:intergenic 50.0%
ATGGATGTCAATGTGAGCGG+TGG + chr6_2:5627182-5627201 Msa0907020:CDS 50.0%
ACGACCTCCACTTTGTTACC+AGG - chr6_2:5627482-5627501 None:intergenic 50.0%
TCACTGTCTGACAAACACCC+CGG + chr6_2:5626212-5626231 Msa0907020:five_prime_UTR 50.0%
AGGCAAGCATGAAAAGTCCG+GGG - chr6_2:5626232-5626251 None:intergenic 50.0%
TGTATCTCCCACTACCAGCT+CGG + chr6_2:5626587-5626606 Msa0907020:five_prime_UTR 50.0%
TCAAGTTCCGAGCTGGTAGT+GGG - chr6_2:5626597-5626616 None:intergenic 50.0%
! TTCAAGTTCCGAGCTGGTAG+TGG - chr6_2:5626598-5626617 None:intergenic 50.0%
ACACACAAACATTTGGGGGG+CGG - chr6_2:5626797-5626816 None:intergenic 50.0%
ATGGATGTCAATGTGAGCGG+TGG + chr6_2:5627182-5627201 Msa0907020:CDS 50.0%
ACGACCTCCACTTTGTTACC+AGG - chr6_2:5627482-5627501 None:intergenic 50.0%
CACACAAACATTTGGGGGGC+GGG - chr6_2:5626796-5626815 None:intergenic 55.0%
CTGCCCGTCCTCATCTTCAA+AGG - chr6_2:5627438-5627457 None:intergenic 55.0%
TTGAAGATGAGGACGGGCAG+AGG + chr6_2:5627438-5627457 Msa0907020:CDS 55.0%
CACACAAACATTTGGGGGGC+GGG - chr6_2:5626796-5626815 None:intergenic 55.0%
CTGCCCGTCCTCATCTTCAA+AGG - chr6_2:5627438-5627457 None:intergenic 55.0%
TTGAAGATGAGGACGGGCAG+AGG + chr6_2:5627438-5627457 Msa0907020:CDS 55.0%
AGCGGTGGTTGCTTCTTCCC+CGG + chr6_2:5627197-5627216 Msa0907020:CDS 60.0%
AGCGGTGGTTGCTTCTTCCC+CGG + chr6_2:5627197-5627216 Msa0907020:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 5626176 5627904 5626176 ID=Msa0907020;Name=Msa0907020
chr6_2 mRNA 5626176 5627904 5626176 ID=Msa0907020-mRNA-1;Parent=Msa0907020;Name=Msa0907020-mRNA-1;_AED=0.39;_eAED=0.39;_QI=552|1|1|1|0|0|2|183|195
chr6_2 exon 5626176 5626772 5626176 ID=Msa0907020-mRNA-1:exon:1724;Parent=Msa0907020-mRNA-1
chr6_2 exon 5627179 5627904 5627179 ID=Msa0907020-mRNA-1:exon:1725;Parent=Msa0907020-mRNA-1
chr6_2 five_prime_UTR 5626176 5626727 5626176 ID=Msa0907020-mRNA-1:five_prime_utr;Parent=Msa0907020-mRNA-1
chr6_2 CDS 5626728 5626772 5626728 ID=Msa0907020-mRNA-1:cds;Parent=Msa0907020-mRNA-1
chr6_2 CDS 5627179 5627721 5627179 ID=Msa0907020-mRNA-1:cds;Parent=Msa0907020-mRNA-1
chr6_2 three_prime_UTR 5627722 5627904 5627722 ID=Msa0907020-mRNA-1:three_prime_utr;Parent=Msa0907020-mRNA-1
Gene Sequence

>Msa0907020

ATGAATTGCGAAGTCACTAATATTTTGAAAGAGATAATTTTACAGAATATGGATGTCAATGTGAGCGGTGGTTGCTTCTTCCCCGGTGATAGTTATCCAAATTGGTTAACCTTTTATTCTGAATGTTCTTCTCTAACTTTTGAAGTCCCTCAAGTGGAAGGGCATAACTTGAAGACAATAATGTGCATAGTCTATACTTCAACCATGGACAACATAACATCAGAATGCCTTAAATGTCTGTTAGTGAAAAATTACACGAAGGCCACCATTCAGCTCTATAAGTGTAATAATACATTAGTTTCCTTTGAAGATGAGGACGGGCAGAGGTTAGTATCAAGTATAGAACCTGGTAACAAAGTGGAGGTCGTTTTTGTTTTTGAGAATGATTTCATTGTGAAGAAGACAACAATTTATCTCGTATACGATAAACCAGTTTCAAAAACATTGGAGTTGTATCACATACCAGATTCGAATTCAATTGCTTGTATTAATGAAGAAAATGAACGCCCTGTCAAGAGATTCTCTACTGAAGAGGAGCCCACTGATGACTTCAATCAGAACAGAAAAAAGAAAAGCCGTGTGGAATGA
Protein sequence

>Msa0907020

MNCEVTNILKEIILQNMDVNVSGGCFFPGDSYPNWLTFYSECSSLTFEVPQVEGHNLKTIMCIVYTSTMDNITSECLKCLLVKNYTKATIQLYKCNNTLVSFEDEDGQRLVSSIEPGNKVEVVFVFENDFIVKKTTIYLVYDKPVSKTLELYHIPDSNSIACINEENERPVKRFSTEEEPTDDFNQNRKKKSRVE*