AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0907100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0907100 MtrunA17_Chr6g0454911 68.534 232 42 3 2 228 61 266 3.59e-106 306
Msa0907100 MtrunA17_Chr6g0454951 66.942 242 64 3 2 228 910 1150 4.02e-102 320
Msa0907100 MtrunA17_Chr6g0455251 68.534 232 53 4 2 228 951 1167 1.44e-95 302
Msa0907100 MtrunA17_Chr6g0455191 73.558 208 39 3 21 228 1175 1366 9.02e-94 299
Msa0907100 MtrunA17_Chr6g0455221 61.200 250 61 4 3 228 921 1158 3.84e-88 282
Msa0907100 MtrunA17_Chr6g0455211 67.839 199 58 2 2 195 868 1065 1.39e-87 279
Msa0907100 MtrunA17_Chr6g0454971 71.078 204 47 2 25 228 894 1085 1.84e-86 276
Msa0907100 MtrunA17_Chr3g0135571 67.598 179 57 1 50 228 1 178 8.19e-82 242
Msa0907100 MtrunA17_Chr3g0135541 67.778 180 57 1 49 228 152 330 2.91e-81 246
Msa0907100 MtrunA17_Chr3g0135511 67.039 179 58 1 50 228 1 178 9.80e-81 239
Msa0907100 MtrunA17_Chr7g0257531 60.697 201 77 2 2 201 488 687 1.30e-76 244
Msa0907100 MtrunA17_Chr6g0454851 62.371 194 72 1 14 207 943 1135 7.00e-76 248
Msa0907100 MtrunA17_Chr6g0455131 60.488 205 56 3 21 224 951 1131 1.03e-69 231
Msa0907100 MtrunA17_Chr6g0454961 49.817 273 83 6 3 224 852 1121 1.06e-68 228
Msa0907100 MtrunA17_Chr6g0455551 57.339 218 67 4 3 195 900 1116 1.40e-67 224
Msa0907100 MtrunA17_Chr4g0005851 51.082 231 95 2 11 224 866 1095 6.77e-67 223
Msa0907100 MtrunA17_Chr4g0014821 62.424 165 60 2 56 220 3 165 7.52e-65 199
Msa0907100 MtrunA17_Chr6g0455031 55.372 242 78 6 2 228 848 1074 2.30e-61 207
Msa0907100 MtrunA17_Chr4g0005591 47.009 234 102 3 10 224 905 1135 3.31e-61 206
Msa0907100 MtrunA17_Chr6g0454821 47.232 271 84 7 3 223 865 1126 4.12e-60 203
Msa0907100 MtrunA17_Chr4g0005871 46.983 232 101 3 11 224 955 1182 7.01e-60 203
Msa0907100 MtrunA17_Chr6g0454831 58.378 185 74 2 9 191 851 1034 4.74e-59 200
Msa0907100 MtrunA17_Chr4g0010711 54.444 180 80 1 21 200 1369 1546 2.29e-57 196
Msa0907100 MtrunA17_Chr4g0005891 46.559 247 106 4 2 224 864 1108 1.36e-55 191
Msa0907100 MtrunA17_Chr7g0234901 50.725 207 76 3 1 183 340 544 1.14e-54 184
Msa0907100 MtrunA17_Chr7g0255681 52.736 201 69 4 1 177 1832 2030 1.25e-52 182
Msa0907100 MtrunA17_Chr6g0454861 66.176 136 20 2 93 228 1 110 4.83e-52 164
Msa0907100 MtrunA17_Chr3g0114381 42.690 171 96 2 14 183 76 245 3.71e-41 142
Msa0907100 MtrunA17_Chr4g0009041 48.538 171 83 5 17 186 1356 1522 8.24e-40 145
Msa0907100 MtrunA17_Chr4g0009241 45.989 187 95 4 1 183 1351 1535 9.26e-40 145
Msa0907100 MtrunA17_Chr8g0352211 46.707 167 77 3 14 179 781 936 7.81e-39 142
Msa0907100 MtrunA17_Chr5g0420341 37.118 229 125 6 4 226 828 1043 2.02e-38 141
Msa0907100 MtrunA17_Chr8g0338881 42.442 172 96 3 14 183 871 1041 1.26e-37 139
Msa0907100 MtrunA17_Chr4g0009401 41.176 204 114 5 18 219 1349 1548 4.43e-37 137
Msa0907100 MtrunA17_Chr4g0009141 38.073 218 108 5 1 193 1326 1541 6.22e-37 137
Msa0907100 MtrunA17_Chr4g0009011 39.437 213 103 5 9 200 109 316 1.11e-36 132
Msa0907100 MtrunA17_Chr4g0009171 45.143 175 92 4 28 200 1582 1754 1.33e-36 136
Msa0907100 MtrunA17_Chr6g0455141 71.951 82 23 0 147 228 1 82 2.78e-36 122
Msa0907100 MtrunA17_Chr2g0296451 36.842 228 133 5 4 221 346 572 3.20e-36 134
Msa0907100 MtrunA17_Chr4g0009101 39.623 212 102 5 11 201 1359 1565 3.88e-36 135
Msa0907100 MtrunA17_Chr8g0338981 39.459 185 105 4 4 183 827 1009 1.28e-35 133
Msa0907100 MtrunA17_Chr4g0009301 40.299 201 106 6 18 208 1389 1585 1.46e-35 133
Msa0907100 MtrunA17_Chr5g0420331 37.949 195 111 5 4 192 828 1018 5.07e-35 131
Msa0907100 MtrunA17_Chr7g0223531 41.622 185 102 3 4 183 843 1026 1.18e-34 130
Msa0907100 MtrunA17_Chr8g0339011 40.323 186 102 6 4 183 823 1005 1.70e-34 130
Msa0907100 MtrunA17_Chr8g0352221 42.690 171 95 3 15 183 758 927 3.57e-34 129
Msa0907100 MtrunA17_Chr8g0338901 38.919 185 106 4 4 183 850 1032 3.93e-34 129
Msa0907100 MtrunA17_Chr8g0339031 36.957 184 113 3 1 183 836 1017 6.81e-34 128
Msa0907100 MtrunA17_Chr8g0339021 38.021 192 109 5 4 189 817 1004 7.99e-34 128
Msa0907100 MtrunA17_Chr5g0414761 39.459 185 104 5 4 183 615 796 8.12e-34 128
Msa0907100 MtrunA17_Chr8g0370511 36.818 220 126 7 4 216 827 1040 1.96e-33 127
Msa0907100 MtrunA17_Chr3g0085731 38.919 185 107 3 4 183 835 1018 5.46e-32 122
Msa0907100 MtrunA17_Chr8g0338931 40.288 139 81 2 46 183 11 148 2.06e-31 116
Msa0907100 MtrunA17_Chr8g0338841 37.222 180 110 3 1 178 271 449 3.50e-31 119
Msa0907100 MtrunA17_Chr3g0085531 38.919 185 104 4 4 183 28 208 4.53e-31 116
Msa0907100 MtrunA17_Chr6g0455181 51.304 115 41 4 2 111 216 320 8.03e-29 110
Msa0907100 MtrunA17_Chr4g0009361 42.857 161 87 5 17 174 360 518 1.05e-28 113
Msa0907100 MtrunA17_Chr6g0454881 60.000 80 31 1 114 193 1 79 4.58e-28 102
Msa0907100 MtrunA17_Chr4g0009211 47.009 117 60 2 85 200 1 116 6.90e-28 103
Msa0907100 MtrunA17_Chr8g0338801 39.062 128 73 4 61 186 86 210 9.06e-24 97.1
Msa0907100 MtrunA17_Chr4g0008981 38.129 139 83 3 85 221 1 138 1.80e-22 89.7
Msa0907100 MtrunA17_Chr5g0430541 33.511 188 105 6 4 184 345 519 3.10e-22 94.7
Msa0907100 MtrunA17_Chr8g0343781 47.436 78 40 1 93 170 5 81 5.23e-18 75.9
Msa0907100 MtrunA17_Chr6g0454871 69.231 52 11 1 2 48 216 267 2.77e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTTTAGATTTATTATTTC+TGG 0.050338 6_2:-5698920 Msa0907100:five_prime_UTR
GAATAAAAGGTTAACCAATT+TGG 0.156402 6_2:+5698215 None:intergenic
AGAAGAACATTCAGAATAAA+AGG 0.229562 6_2:+5698202 None:intergenic
TTCAACTTATAGATACTTCT+TGG 0.270204 6_2:+5699085 None:intergenic
TTGTTTAAATATTGATATAT+TGG 0.286266 6_2:-5699035 Msa0907100:five_prime_UTR
CCGGTGATAGTTATCCAAAT+TGG 0.355985 6_2:-5698229 Msa0907100:CDS
GTGACTGCAAACTTGTTTAT+CGG 0.375374 6_2:-5697674 Msa0907100:three_prime_UTR
TCAAAGGTTTCTCCTACAAT+TGG 0.385254 6_2:-5699166 Msa0907100:five_prime_UTR
CAAGCAATTGAATTCGAATC+TGG 0.407064 6_2:+5697849 None:intergenic
TCAACTTATAGATACTTCTT+GGG 0.411779 6_2:+5699086 None:intergenic
CTAGAAACTTCAGCCAGAAA+TGG 0.412434 6_2:+5697638 None:intergenic
TGATATATTGGAAGAGGACT+TGG 0.413185 6_2:-5699023 Msa0907100:five_prime_UTR
ACTTTAGATTTATTATTTCT+GGG 0.413861 6_2:-5698919 Msa0907100:five_prime_UTR
GGAAGAGGACTTGGAACAAA+TGG 0.414502 6_2:-5699014 Msa0907100:five_prime_UTR
GATCTACTTAGCAGCAAGAT+TGG 0.430014 6_2:+5697568 None:intergenic
TTAAAAGAGACTTCAAGAAA+TGG 0.432195 6_2:+5698751 None:intergenic
TTTGACCTTGCAACTGAAAA+GGG 0.438981 6_2:+5698944 None:intergenic
TTAGGCAAGCATGAAAAGTC+CGG 0.482068 6_2:+5699226 None:intergenic
TTCTGATGTTATGTTGTCCA+TGG 0.488711 6_2:+5698109 None:intergenic
AAATATTGATATATTGGAAG+AGG 0.493151 6_2:-5699029 Msa0907100:five_prime_UTR
TCAGTAGAGAATCTCTTGAC+AGG 0.493870 6_2:+5697804 None:intergenic
ATTTGTGATGTAGTTGCAGT+TGG 0.494996 6_2:+5698780 None:intergenic
AGTTATGCCCTTCCACTTGA+GGG 0.503447 6_2:+5698165 None:intergenic
GGAGAAACCTTTGATAACAT+TGG 0.513337 6_2:+5699175 None:intergenic
AGCGGTGGTTGCTTCTTCCC+CGG 0.514887 6_2:-5698248 Msa0907100:CDS
CCAATTTGGATAACTATCAC+CGG 0.519080 6_2:+5698229 None:intergenic
TACTTCTTGGGAGGCTACAT+AGG 0.520724 6_2:+5699098 None:intergenic
TTCTGATTGAAGTCATCAGT+GGG 0.523114 6_2:+5697774 None:intergenic
ATTACACTTATAGAGCTGAA+TGG 0.524275 6_2:+5698046 None:intergenic
AAGTTATGCCCTTCCACTTG+AGG 0.525281 6_2:+5698164 None:intergenic
ACGACCTCCACTTTGTTACC+AGG 0.527935 6_2:+5697966 None:intergenic
ACTTGAACTTTCTCATCAGA+TGG 0.530954 6_2:-5698835 Msa0907100:exon
ATTAGTTTCCTTTGAAGATG+AGG 0.532317 6_2:-5698018 Msa0907100:CDS
TTTGAAGTCCCTCAAGTGGA+AGG 0.532396 6_2:-5698173 Msa0907100:CDS
GTTCTGATTGAAGTCATCAG+TGG 0.549628 6_2:+5697773 None:intergenic
AGATTGTCCAATGTTATCAA+AGG 0.554207 6_2:-5699182 Msa0907100:five_prime_UTR
CAAGAGATTCTCTACTGAAG+AGG 0.554484 6_2:-5697799 Msa0907100:CDS
CTGCCCGTCCTCATCTTCAA+AGG 0.557946 6_2:+5698010 None:intergenic
CAATTTGGATAACTATCACC+GGG 0.558896 6_2:+5698230 None:intergenic
CATAGTCTATACTTCAACCA+TGG 0.564030 6_2:-5698126 Msa0907100:CDS
CTCACTGTCTGACAAACACC+CGG 0.570281 6_2:-5699245 Msa0907100:five_prime_UTR
TTGAAGATGAGGACGGGCAG+AGG 0.570651 6_2:-5698007 Msa0907100:CDS
TAGGCAAGCATGAAAAGTCC+GGG 0.579313 6_2:+5699227 None:intergenic
TGTAGTTGCAGTTGGTTGCA+AGG 0.580339 6_2:+5698788 None:intergenic
AGAAAGTTCAAGTTCCGAGC+TGG 0.586660 6_2:+5698845 None:intergenic
GTTTCCTTTGAAGATGAGGA+CGG 0.588818 6_2:-5698014 Msa0907100:CDS
ACTTATAGATACTTCTTGGG+AGG 0.588957 6_2:+5699089 None:intergenic
GATGAAAGATAGTAAAGCAA+TGG 0.597129 6_2:-5697720 Msa0907100:three_prime_UTR
TTGAAGTCCCTCAAGTGGAA+GGG 0.598230 6_2:-5698172 Msa0907100:CDS
AAATCTTTCAACAGTCACCA+TGG 0.605119 6_2:+5697608 None:intergenic
CAGTAGAGAATCTCTTGACA+GGG 0.607184 6_2:+5697805 None:intergenic
ACATATCTCAGCTACCAGCT+CGG 0.610539 6_2:-5698859 Msa0907100:five_prime_UTR
TTTCCTTTGAAGATGAGGAC+GGG 0.614409 6_2:-5698013 Msa0907100:CDS
TATAGAACCTGGTAACAAAG+TGG 0.620153 6_2:-5697973 Msa0907100:CDS
TTTCATCGATTCATTCCACA+CGG 0.653854 6_2:+5697736 None:intergenic
TTAGTATCAAGTATAGAACC+TGG 0.675876 6_2:-5697984 Msa0907100:CDS
ACACTTATAGAGCTGAATGG+TGG 0.682446 6_2:+5698049 None:intergenic
AGTGTTATTTGCACGCAGAG+TGG 0.684066 6_2:+5698982 None:intergenic
ATGGATGTCAATGTGAGCGG+TGG 0.702164 6_2:-5698263 Msa0907100:CDS
AGAACCTGGTAACAAAGTGG+AGG 0.707895 6_2:-5697970 Msa0907100:CDS
AATATGGATGTCAATGTGAG+CGG 0.747306 6_2:-5698266 Msa0907100:CDS
AATTTGGATAACTATCACCG+GGG 0.761873 6_2:+5698231 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTTAAATATTGATATAT+TGG - chr6_2:5697808-5697827 Msa0907100:CDS 10.0%
!!! AAATACTTTTTTTTTATCAA+AGG - chr6_2:5698299-5698318 Msa0907100:intron 10.0%
!! TTGTTTAAATATTGATATAT+TGG - chr6_2:5697808-5697827 Msa0907100:CDS 10.0%
!!! AAATACTTTTTTTTTATCAA+AGG - chr6_2:5698299-5698318 Msa0907100:intron 10.0%
!!! AAAAATTCTGTTGATAAATT+TGG - chr6_2:5697709-5697728 Msa0907100:three_prime_UTR 15.0%
!!! ACTTTAGATTTATTATTTCT+GGG - chr6_2:5697924-5697943 Msa0907100:CDS 15.0%
!!! AAAAATTCTGTTGATAAATT+TGG - chr6_2:5697709-5697728 Msa0907100:three_prime_UTR 15.0%
!!! ACTTTAGATTTATTATTTCT+GGG - chr6_2:5697924-5697943 Msa0907100:CDS 15.0%
!! AAATATTGATATATTGGAAG+AGG - chr6_2:5697814-5697833 Msa0907100:CDS 20.0%
!! GACTTTAGATTTATTATTTC+TGG - chr6_2:5697923-5697942 Msa0907100:CDS 20.0%
!!! AAGTCTCTTTTAATTCAAAT+TGG - chr6_2:5698102-5698121 Msa0907100:CDS 20.0%
!!! TTTGAAAGAGATAATTTTAC+AGG - chr6_2:5698149-5698168 Msa0907100:CDS 20.0%
!! TGTATATGATTATTTGCATT+AGG - chr6_2:5698272-5698291 Msa0907100:intron 20.0%
!! TATTTGTTTAATGAATGACA+AGG - chr6_2:5698506-5698525 Msa0907100:intron 20.0%
!! AAATATTGATATATTGGAAG+AGG - chr6_2:5697814-5697833 Msa0907100:CDS 20.0%
!! GACTTTAGATTTATTATTTC+TGG - chr6_2:5697923-5697942 Msa0907100:CDS 20.0%
!!! AAGTCTCTTTTAATTCAAAT+TGG - chr6_2:5698102-5698121 Msa0907100:CDS 20.0%
!!! TTTGAAAGAGATAATTTTAC+AGG - chr6_2:5698149-5698168 Msa0907100:CDS 20.0%
!! TGTATATGATTATTTGCATT+AGG - chr6_2:5698272-5698291 Msa0907100:intron 20.0%
!! TATTTGTTTAATGAATGACA+AGG - chr6_2:5698506-5698525 Msa0907100:intron 20.0%
! TCAACTTATAGATACTTCTT+GGG + chr6_2:5697760-5697779 None:intergenic 25.0%
! TTCAACTTATAGATACTTCT+TGG + chr6_2:5697761-5697780 None:intergenic 25.0%
! CTAAAAACTTTGATTGTTTC+TGG - chr6_2:5697785-5697804 Msa0907100:CDS 25.0%
! TTAAAAGAGACTTCAAGAAA+TGG + chr6_2:5698095-5698114 None:intergenic 25.0%
!! ATCCAAATTCTCACAAATTT+TGG - chr6_2:5698379-5698398 Msa0907100:intron 25.0%
! TACCAAAATTTGTGAGAATT+TGG + chr6_2:5698384-5698403 None:intergenic 25.0%
! GAATAAAAGGTTAACCAATT+TGG + chr6_2:5698631-5698650 None:intergenic 25.0%
! AGAAGAACATTCAGAATAAA+AGG + chr6_2:5698644-5698663 None:intergenic 25.0%
!! TTTTCACTAACAGACATTTA+AGG + chr6_2:5698762-5698781 None:intergenic 25.0%
! TAAACCAGTTTCAAAAACAT+TGG - chr6_2:5698957-5698976 Msa0907100:five_prime_UTR 25.0%
!!! CATAAGAATTGATTTTTGCT+TGG + chr6_2:5699149-5699168 None:intergenic 25.0%
! CAAGCAAAAATCAATTCTTA+TGG - chr6_2:5699147-5699166 Msa0907100:five_prime_UTR 25.0%
!!! AAGTTTCTAGATTTTATCCA+TGG - chr6_2:5699218-5699237 Msa0907100:five_prime_UTR 25.0%
! TCAACTTATAGATACTTCTT+GGG + chr6_2:5697760-5697779 None:intergenic 25.0%
! TTCAACTTATAGATACTTCT+TGG + chr6_2:5697761-5697780 None:intergenic 25.0%
! CTAAAAACTTTGATTGTTTC+TGG - chr6_2:5697785-5697804 Msa0907100:CDS 25.0%
! TTAAAAGAGACTTCAAGAAA+TGG + chr6_2:5698095-5698114 None:intergenic 25.0%
!! ATCCAAATTCTCACAAATTT+TGG - chr6_2:5698379-5698398 Msa0907100:intron 25.0%
! TACCAAAATTTGTGAGAATT+TGG + chr6_2:5698384-5698403 None:intergenic 25.0%
! GAATAAAAGGTTAACCAATT+TGG + chr6_2:5698631-5698650 None:intergenic 25.0%
! AGAAGAACATTCAGAATAAA+AGG + chr6_2:5698644-5698663 None:intergenic 25.0%
!! TTTTCACTAACAGACATTTA+AGG + chr6_2:5698762-5698781 None:intergenic 25.0%
! TAAACCAGTTTCAAAAACAT+TGG - chr6_2:5698957-5698976 Msa0907100:five_prime_UTR 25.0%
!!! CATAAGAATTGATTTTTGCT+TGG + chr6_2:5699149-5699168 None:intergenic 25.0%
! CAAGCAAAAATCAATTCTTA+TGG - chr6_2:5699147-5699166 Msa0907100:five_prime_UTR 25.0%
!!! AAGTTTCTAGATTTTATCCA+TGG - chr6_2:5699218-5699237 Msa0907100:five_prime_UTR 25.0%
!!! AGTACTAGCTTTTCAAGATT+AGG + chr6_2:5697638-5697657 None:intergenic 30.0%
AGATTGTCCAATGTTATCAA+AGG - chr6_2:5697661-5697680 Msa0907100:three_prime_UTR 30.0%
! TTTTTCTGATGTCCAATTGT+AGG + chr6_2:5697692-5697711 None:intergenic 30.0%
! TCATCAGATGGCATTATTTT+TGG - chr6_2:5698020-5698039 Msa0907100:CDS 30.0%
TTAAGAGTTCAAATGTAGGA+GGG + chr6_2:5698223-5698242 None:intergenic 30.0%
ACACTTAAGAGTTCAAATGT+AGG + chr6_2:5698227-5698246 None:intergenic 30.0%
TTGTTTAATGAATGACAAGG+TGG - chr6_2:5698509-5698528 Msa0907100:intron 30.0%
ATCTCTACTTTCACAGAATA+TGG - chr6_2:5698561-5698580 Msa0907100:intron 30.0%
GTTAGTGAAAAATTACACGA+AGG - chr6_2:5698771-5698790 Msa0907100:CDS 30.0%
ATTACACTTATAGAGCTGAA+TGG + chr6_2:5698800-5698819 None:intergenic 30.0%
! ATTAGTTTCCTTTGAAGATG+AGG - chr6_2:5698825-5698844 Msa0907100:exon 30.0%
TTAGTATCAAGTATAGAACC+TGG - chr6_2:5698859-5698878 Msa0907100:five_prime_UTR 30.0%
!!! AACTCCAATGTTTTTGAAAC+TGG + chr6_2:5698964-5698983 None:intergenic 30.0%
GATGAAAGATAGTAAAGCAA+TGG - chr6_2:5699123-5699142 Msa0907100:five_prime_UTR 30.0%
! CAACAATTGTTTTCCATTTC+TGG - chr6_2:5699192-5699211 Msa0907100:five_prime_UTR 30.0%
!!! AGTACTAGCTTTTCAAGATT+AGG + chr6_2:5697638-5697657 None:intergenic 30.0%
AGATTGTCCAATGTTATCAA+AGG - chr6_2:5697661-5697680 Msa0907100:three_prime_UTR 30.0%
! TTTTTCTGATGTCCAATTGT+AGG + chr6_2:5697692-5697711 None:intergenic 30.0%
! TCATCAGATGGCATTATTTT+TGG - chr6_2:5698020-5698039 Msa0907100:CDS 30.0%
TTAAGAGTTCAAATGTAGGA+GGG + chr6_2:5698223-5698242 None:intergenic 30.0%
ACACTTAAGAGTTCAAATGT+AGG + chr6_2:5698227-5698246 None:intergenic 30.0%
TTGTTTAATGAATGACAAGG+TGG - chr6_2:5698509-5698528 Msa0907100:intron 30.0%
ATCTCTACTTTCACAGAATA+TGG - chr6_2:5698561-5698580 Msa0907100:intron 30.0%
GTTAGTGAAAAATTACACGA+AGG - chr6_2:5698771-5698790 Msa0907100:CDS 30.0%
ATTACACTTATAGAGCTGAA+TGG + chr6_2:5698800-5698819 None:intergenic 30.0%
! ATTAGTTTCCTTTGAAGATG+AGG - chr6_2:5698825-5698844 Msa0907100:exon 30.0%
TTAGTATCAAGTATAGAACC+TGG - chr6_2:5698859-5698878 Msa0907100:five_prime_UTR 30.0%
!!! AACTCCAATGTTTTTGAAAC+TGG + chr6_2:5698964-5698983 None:intergenic 30.0%
GATGAAAGATAGTAAAGCAA+TGG - chr6_2:5699123-5699142 Msa0907100:five_prime_UTR 30.0%
! CAACAATTGTTTTCCATTTC+TGG - chr6_2:5699192-5699211 Msa0907100:five_prime_UTR 30.0%
! GGAGAAACCTTTGATAACAT+TGG + chr6_2:5697671-5697690 None:intergenic 35.0%
TCAAAGGTTTCTCCTACAAT+TGG - chr6_2:5697677-5697696 Msa0907100:three_prime_UTR 35.0%
ACTTATAGATACTTCTTGGG+AGG + chr6_2:5697757-5697776 None:intergenic 35.0%
TGATATATTGGAAGAGGACT+TGG - chr6_2:5697820-5697839 Msa0907100:CDS 35.0%
TTTGACCTTGCAACTGAAAA+GGG + chr6_2:5697902-5697921 None:intergenic 35.0%
! TTTTGACCTTGCAACTGAAA+AGG + chr6_2:5697903-5697922 None:intergenic 35.0%
ACTTGAACTTTCTCATCAGA+TGG - chr6_2:5698008-5698027 Msa0907100:CDS 35.0%
ATTTGTGATGTAGTTGCAGT+TGG + chr6_2:5698066-5698085 None:intergenic 35.0%
ATACACACACAAACATTTGG+GGG + chr6_2:5698199-5698218 None:intergenic 35.0%
GATACACACACAAACATTTG+GGG + chr6_2:5698200-5698219 None:intergenic 35.0%
GGATACACACACAAACATTT+GGG + chr6_2:5698201-5698220 None:intergenic 35.0%
TAAGAGTTCAAATGTAGGAG+GGG + chr6_2:5698222-5698241 None:intergenic 35.0%
CTTAAGAGTTCAAATGTAGG+AGG + chr6_2:5698224-5698243 None:intergenic 35.0%
AATATGGATGTCAATGTGAG+CGG - chr6_2:5698577-5698596 Msa0907100:intron 35.0%
AATTTGGATAACTATCACCG+GGG + chr6_2:5698615-5698634 None:intergenic 35.0%
CAATTTGGATAACTATCACC+GGG + chr6_2:5698616-5698635 None:intergenic 35.0%
CCAATTTGGATAACTATCAC+CGG + chr6_2:5698617-5698636 None:intergenic 35.0%
CATAGTCTATACTTCAACCA+TGG - chr6_2:5698717-5698736 Msa0907100:CDS 35.0%
TTCTGATGTTATGTTGTCCA+TGG + chr6_2:5698737-5698756 None:intergenic 35.0%
TATAGAACCTGGTAACAAAG+TGG - chr6_2:5698870-5698889 Msa0907100:five_prime_UTR 35.0%
CAAGCAATTGAATTCGAATC+TGG + chr6_2:5698997-5699016 None:intergenic 35.0%
TTCTGATTGAAGTCATCAGT+GGG + chr6_2:5699072-5699091 None:intergenic 35.0%
!! TTTCATCGATTCATTCCACA+CGG + chr6_2:5699110-5699129 None:intergenic 35.0%
! GTGACTGCAAACTTGTTTAT+CGG - chr6_2:5699169-5699188 Msa0907100:five_prime_UTR 35.0%
AAATCTTTCAACAGTCACCA+TGG + chr6_2:5699238-5699257 None:intergenic 35.0%
! GGAGAAACCTTTGATAACAT+TGG + chr6_2:5697671-5697690 None:intergenic 35.0%
TCAAAGGTTTCTCCTACAAT+TGG - chr6_2:5697677-5697696 Msa0907100:three_prime_UTR 35.0%
ACTTATAGATACTTCTTGGG+AGG + chr6_2:5697757-5697776 None:intergenic 35.0%
TGATATATTGGAAGAGGACT+TGG - chr6_2:5697820-5697839 Msa0907100:CDS 35.0%
TTTGACCTTGCAACTGAAAA+GGG + chr6_2:5697902-5697921 None:intergenic 35.0%
! TTTTGACCTTGCAACTGAAA+AGG + chr6_2:5697903-5697922 None:intergenic 35.0%
ACTTGAACTTTCTCATCAGA+TGG - chr6_2:5698008-5698027 Msa0907100:CDS 35.0%
ATTTGTGATGTAGTTGCAGT+TGG + chr6_2:5698066-5698085 None:intergenic 35.0%
ATACACACACAAACATTTGG+GGG + chr6_2:5698199-5698218 None:intergenic 35.0%
GATACACACACAAACATTTG+GGG + chr6_2:5698200-5698219 None:intergenic 35.0%
GGATACACACACAAACATTT+GGG + chr6_2:5698201-5698220 None:intergenic 35.0%
TAAGAGTTCAAATGTAGGAG+GGG + chr6_2:5698222-5698241 None:intergenic 35.0%
CTTAAGAGTTCAAATGTAGG+AGG + chr6_2:5698224-5698243 None:intergenic 35.0%
AATATGGATGTCAATGTGAG+CGG - chr6_2:5698577-5698596 Msa0907100:intron 35.0%
AATTTGGATAACTATCACCG+GGG + chr6_2:5698615-5698634 None:intergenic 35.0%
CAATTTGGATAACTATCACC+GGG + chr6_2:5698616-5698635 None:intergenic 35.0%
CCAATTTGGATAACTATCAC+CGG + chr6_2:5698617-5698636 None:intergenic 35.0%
CATAGTCTATACTTCAACCA+TGG - chr6_2:5698717-5698736 Msa0907100:CDS 35.0%
TTCTGATGTTATGTTGTCCA+TGG + chr6_2:5698737-5698756 None:intergenic 35.0%
TATAGAACCTGGTAACAAAG+TGG - chr6_2:5698870-5698889 Msa0907100:five_prime_UTR 35.0%
CAAGCAATTGAATTCGAATC+TGG + chr6_2:5698997-5699016 None:intergenic 35.0%
TTCTGATTGAAGTCATCAGT+GGG + chr6_2:5699072-5699091 None:intergenic 35.0%
!! TTTCATCGATTCATTCCACA+CGG + chr6_2:5699110-5699129 None:intergenic 35.0%
! GTGACTGCAAACTTGTTTAT+CGG - chr6_2:5699169-5699188 Msa0907100:five_prime_UTR 35.0%
AAATCTTTCAACAGTCACCA+TGG + chr6_2:5699238-5699257 None:intergenic 35.0%
TTAGGCAAGCATGAAAAGTC+CGG + chr6_2:5697620-5697639 None:intergenic 40.0%
TACACACACAAACATTTGGG+GGG + chr6_2:5698198-5698217 None:intergenic 40.0%
GGGATACACACACAAACATT+TGG + chr6_2:5698202-5698221 None:intergenic 40.0%
CCGGTGATAGTTATCCAAAT+TGG - chr6_2:5698614-5698633 Msa0907100:intron 40.0%
!!! AACTTTTGAAGTCCCTCAAG+TGG - chr6_2:5698666-5698685 Msa0907100:intron 40.0%
ACACTTATAGAGCTGAATGG+TGG + chr6_2:5698797-5698816 None:intergenic 40.0%
! GTTTCCTTTGAAGATGAGGA+CGG - chr6_2:5698829-5698848 Msa0907100:exon 40.0%
! TTTCCTTTGAAGATGAGGAC+GGG - chr6_2:5698830-5698849 Msa0907100:exon 40.0%
CAGTAGAGAATCTCTTGACA+GGG + chr6_2:5699041-5699060 None:intergenic 40.0%
TCAGTAGAGAATCTCTTGAC+AGG + chr6_2:5699042-5699061 None:intergenic 40.0%
CAAGAGATTCTCTACTGAAG+AGG - chr6_2:5699044-5699063 Msa0907100:five_prime_UTR 40.0%
GTTCTGATTGAAGTCATCAG+TGG + chr6_2:5699073-5699092 None:intergenic 40.0%
CAGAAAAAAGAAAAGCCGTG+TGG - chr6_2:5699092-5699111 Msa0907100:five_prime_UTR 40.0%
CTAGAAACTTCAGCCAGAAA+TGG + chr6_2:5699208-5699227 None:intergenic 40.0%
GATCTACTTAGCAGCAAGAT+TGG + chr6_2:5699278-5699297 None:intergenic 40.0%
TTAGGCAAGCATGAAAAGTC+CGG + chr6_2:5697620-5697639 None:intergenic 40.0%
TACACACACAAACATTTGGG+GGG + chr6_2:5698198-5698217 None:intergenic 40.0%
GGGATACACACACAAACATT+TGG + chr6_2:5698202-5698221 None:intergenic 40.0%
CCGGTGATAGTTATCCAAAT+TGG - chr6_2:5698614-5698633 Msa0907100:intron 40.0%
!!! AACTTTTGAAGTCCCTCAAG+TGG - chr6_2:5698666-5698685 Msa0907100:intron 40.0%
ACACTTATAGAGCTGAATGG+TGG + chr6_2:5698797-5698816 None:intergenic 40.0%
! GTTTCCTTTGAAGATGAGGA+CGG - chr6_2:5698829-5698848 Msa0907100:exon 40.0%
! TTTCCTTTGAAGATGAGGAC+GGG - chr6_2:5698830-5698849 Msa0907100:exon 40.0%
CAGTAGAGAATCTCTTGACA+GGG + chr6_2:5699041-5699060 None:intergenic 40.0%
TCAGTAGAGAATCTCTTGAC+AGG + chr6_2:5699042-5699061 None:intergenic 40.0%
CAAGAGATTCTCTACTGAAG+AGG - chr6_2:5699044-5699063 Msa0907100:five_prime_UTR 40.0%
GTTCTGATTGAAGTCATCAG+TGG + chr6_2:5699073-5699092 None:intergenic 40.0%
CAGAAAAAAGAAAAGCCGTG+TGG - chr6_2:5699092-5699111 Msa0907100:five_prime_UTR 40.0%
CTAGAAACTTCAGCCAGAAA+TGG + chr6_2:5699208-5699227 None:intergenic 40.0%
GATCTACTTAGCAGCAAGAT+TGG + chr6_2:5699278-5699297 None:intergenic 40.0%
TAGGCAAGCATGAAAAGTCC+GGG + chr6_2:5697619-5697638 None:intergenic 45.0%
TACTTCTTGGGAGGCTACAT+AGG + chr6_2:5697748-5697767 None:intergenic 45.0%
GGAAGAGGACTTGGAACAAA+TGG - chr6_2:5697829-5697848 Msa0907100:CDS 45.0%
!! AGTGTTATTTGCACGCAGAG+TGG + chr6_2:5697864-5697883 None:intergenic 45.0%
! GAGTACCCTTTTCAGTTGCA+AGG - chr6_2:5697894-5697913 Msa0907100:CDS 45.0%
ACATATCTCAGCTACCAGCT+CGG - chr6_2:5697984-5698003 Msa0907100:CDS 45.0%
! AGAAAGTTCAAGTTCCGAGC+TGG + chr6_2:5698001-5698020 None:intergenic 45.0%
TGTAGTTGCAGTTGGTTGCA+AGG + chr6_2:5698058-5698077 None:intergenic 45.0%
CTGACAGCTTACTTGATACG+TGG - chr6_2:5698411-5698430 Msa0907100:intron 45.0%
! TTTGAAGTCCCTCAAGTGGA+AGG - chr6_2:5698670-5698689 Msa0907100:intron 45.0%
TTGAAGTCCCTCAAGTGGAA+GGG - chr6_2:5698671-5698690 Msa0907100:intron 45.0%
AGTTATGCCCTTCCACTTGA+GGG + chr6_2:5698681-5698700 None:intergenic 45.0%
AAGTTATGCCCTTCCACTTG+AGG + chr6_2:5698682-5698701 None:intergenic 45.0%
AGAACCTGGTAACAAAGTGG+AGG - chr6_2:5698873-5698892 Msa0907100:five_prime_UTR 45.0%
TAGGCAAGCATGAAAAGTCC+GGG + chr6_2:5697619-5697638 None:intergenic 45.0%
TACTTCTTGGGAGGCTACAT+AGG + chr6_2:5697748-5697767 None:intergenic 45.0%
GGAAGAGGACTTGGAACAAA+TGG - chr6_2:5697829-5697848 Msa0907100:CDS 45.0%
!! AGTGTTATTTGCACGCAGAG+TGG + chr6_2:5697864-5697883 None:intergenic 45.0%
! GAGTACCCTTTTCAGTTGCA+AGG - chr6_2:5697894-5697913 Msa0907100:CDS 45.0%
ACATATCTCAGCTACCAGCT+CGG - chr6_2:5697984-5698003 Msa0907100:CDS 45.0%
! AGAAAGTTCAAGTTCCGAGC+TGG + chr6_2:5698001-5698020 None:intergenic 45.0%
TGTAGTTGCAGTTGGTTGCA+AGG + chr6_2:5698058-5698077 None:intergenic 45.0%
CTGACAGCTTACTTGATACG+TGG - chr6_2:5698411-5698430 Msa0907100:intron 45.0%
! TTTGAAGTCCCTCAAGTGGA+AGG - chr6_2:5698670-5698689 Msa0907100:intron 45.0%
TTGAAGTCCCTCAAGTGGAA+GGG - chr6_2:5698671-5698690 Msa0907100:intron 45.0%
AGTTATGCCCTTCCACTTGA+GGG + chr6_2:5698681-5698700 None:intergenic 45.0%
AAGTTATGCCCTTCCACTTG+AGG + chr6_2:5698682-5698701 None:intergenic 45.0%
AGAACCTGGTAACAAAGTGG+AGG - chr6_2:5698873-5698892 Msa0907100:five_prime_UTR 45.0%
!! ATTTAAAAATAAATATGTTA+AGG + chr6_2:5698355-5698374 None:intergenic 5.0%
!! ATTTAAAAATAAATATGTTA+AGG + chr6_2:5698355-5698374 None:intergenic 5.0%
CTCACTGTCTGACAAACACC+CGG - chr6_2:5697598-5697617 Msa0907100:three_prime_UTR 50.0%
ACACACAAACATTTGGGGGG+CGG + chr6_2:5698195-5698214 None:intergenic 50.0%
ATGGATGTCAATGTGAGCGG+TGG - chr6_2:5698580-5698599 Msa0907100:intron 50.0%
ACGACCTCCACTTTGTTACC+AGG + chr6_2:5698880-5698899 None:intergenic 50.0%
CTCACTGTCTGACAAACACC+CGG - chr6_2:5697598-5697617 Msa0907100:three_prime_UTR 50.0%
ACACACAAACATTTGGGGGG+CGG + chr6_2:5698195-5698214 None:intergenic 50.0%
ATGGATGTCAATGTGAGCGG+TGG - chr6_2:5698580-5698599 Msa0907100:intron 50.0%
ACGACCTCCACTTTGTTACC+AGG + chr6_2:5698880-5698899 None:intergenic 50.0%
CACACAAACATTTGGGGGGC+GGG + chr6_2:5698194-5698213 None:intergenic 55.0%
CTGCCCGTCCTCATCTTCAA+AGG + chr6_2:5698836-5698855 None:intergenic 55.0%
TTGAAGATGAGGACGGGCAG+AGG - chr6_2:5698836-5698855 Msa0907100:exon 55.0%
CACACAAACATTTGGGGGGC+GGG + chr6_2:5698194-5698213 None:intergenic 55.0%
CTGCCCGTCCTCATCTTCAA+AGG + chr6_2:5698836-5698855 None:intergenic 55.0%
TTGAAGATGAGGACGGGCAG+AGG - chr6_2:5698836-5698855 Msa0907100:exon 55.0%
AGCGGTGGTTGCTTCTTCCC+CGG - chr6_2:5698595-5698614 Msa0907100:intron 60.0%
AGCGGTGGTTGCTTCTTCCC+CGG - chr6_2:5698595-5698614 Msa0907100:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 5697563 5699302 5697563 ID=Msa0907100;Name=Msa0907100
chr6_2 mRNA 5697563 5699302 5697563 ID=Msa0907100-mRNA-1;Parent=Msa0907100;Name=Msa0907100-mRNA-1;_AED=0.39;_eAED=0.39;_QI=464|1|1|1|0|0|2|183|228
chr6_2 exon 5697563 5698288 5697563 ID=Msa0907100-mRNA-1:exon:1772;Parent=Msa0907100-mRNA-1
chr6_2 exon 5698695 5699302 5698695 ID=Msa0907100-mRNA-1:exon:1771;Parent=Msa0907100-mRNA-1
chr6_2 five_prime_UTR 5698839 5699302 5698839 ID=Msa0907100-mRNA-1:five_prime_utr;Parent=Msa0907100-mRNA-1
chr6_2 CDS 5698695 5698838 5698695 ID=Msa0907100-mRNA-1:cds;Parent=Msa0907100-mRNA-1
chr6_2 CDS 5697746 5698288 5697746 ID=Msa0907100-mRNA-1:cds;Parent=Msa0907100-mRNA-1
chr6_2 three_prime_UTR 5697563 5697745 5697563 ID=Msa0907100-mRNA-1:three_prime_utr;Parent=Msa0907100-mRNA-1
Gene Sequence

>Msa0907100

ATGGCATTATTTTTGGATGATTTGCATGCCTTGCAACCAACTGCAACTACATCACAAATATCAAACCATTTCTTGAAGTCTCTTTTAATTCAAATTGGAATGAATTGCGAAGTCACTAATATTTTGAAAGAGATAATTTTACAGAATATGGATGTCAATGTGAGCGGTGGTTGCTTCTTCCCCGGTGATAGTTATCCAAATTGGTTAACCTTTTATTCTGAATGTTCTTCTCTAACTTTTGAAGTCCCTCAAGTGGAAGGGCATAACTTGAAGACAATAATGTGCATAGTCTATACTTCAACCATGGACAACATAACATCAGAATGCCTTAAATGTCTGTTAGTGAAAAATTACACGAAGGCCACCATTCAGCTCTATAAGTGTAATAATACATTAGTTTCCTTTGAAGATGAGGACGGGCAGAGGTTAGTATCAAGTATAGAACCTGGTAACAAAGTGGAGGTCGTTTTTGTTTTTGAGAATGATTTCATTGTGAAGAAGACAACAATTTATCTCGTATACGATAAACCAGTTTCAAAAACATTGGAGTTGTATCACATACCAGATTCGAATTCAATTGCTTGTATTAATGAAGAAAATGAACGCCCTGTCAAGAGATTCTCTACTGAAGAGGAGCCCACTGATGACTTCAATCAGAACAGAAAAAAGAAAAGCCGTGTGGAATGA
Protein sequence

>Msa0907100

MALFLDDLHALQPTATTSQISNHFLKSLLIQIGMNCEVTNILKEIILQNMDVNVSGGCFFPGDSYPNWLTFYSECSSLTFEVPQVEGHNLKTIMCIVYTSTMDNITSECLKCLLVKNYTKATIQLYKCNNTLVSFEDEDGQRLVSSIEPGNKVEVVFVFENDFIVKKTTIYLVYDKPVSKTLELYHIPDSNSIACINEENERPVKRFSTEEEPTDDFNQNRKKKSRVE*