AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0912180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0912180 MtrunA17_Chr2g0297261 91.451 386 27 1 1 380 21 406 0.0 729
Msa0912180 MtrunA17_Chr2g0299681 63.003 373 132 1 14 380 41 413 7.85e-175 493
Msa0912180 MtrunA17_Chr2g0329931 60.204 392 143 2 2 380 22 413 1.03e-172 488
Msa0912180 MtrunA17_Chr8g0342861 61.662 373 137 1 14 380 41 413 9.05e-172 485
Msa0912180 MtrunA17_Chr4g0070981 62.198 373 135 1 14 380 41 413 1.09e-170 482
Msa0912180 MtrunA17_Chr1g0169331 41.878 394 186 6 29 380 6 398 8.64e-101 304
Msa0912180 MtrunA17_Chr8g0342851 42.486 346 163 4 43 380 2 319 5.38e-89 271
Msa0912180 MtrunA17_Chr7g0247191 38.630 365 216 3 15 373 151 513 3.16e-85 268
Msa0912180 MtrunA17_Chr7g0247181 38.082 365 218 3 15 373 141 503 3.55e-85 267
Msa0912180 MtrunA17_Chr7g0247221 38.082 365 218 3 15 373 144 506 5.64e-85 267
Msa0912180 MtrunA17_Chr2g0330401 33.333 372 233 5 5 361 84 455 3.82e-69 225
Msa0912180 MtrunA17_Chr4g0003441 35.897 390 230 9 5 376 93 480 5.27e-69 225
Msa0912180 MtrunA17_Chr1g0179761 33.753 397 227 10 11 372 77 472 4.04e-61 204
Msa0912180 MtrunA17_Chr1g0149251 36.228 334 199 8 52 373 2 333 5.06e-61 200
Msa0912180 MtrunA17_Chr7g0270131 32.653 392 241 7 3 374 224 612 8.46e-61 209
Msa0912180 MtrunA17_Chr2g0293631 32.432 370 230 6 14 364 496 864 1.27e-60 211
Msa0912180 MtrunA17_Chr5g0442191 31.117 376 242 5 17 378 84 456 4.09e-59 201
Msa0912180 MtrunA17_Chr3g0107851 32.353 374 229 7 12 363 115 486 4.45e-59 201
Msa0912180 MtrunA17_Chr3g0121351 32.010 403 237 8 4 373 133 531 5.95e-59 201
Msa0912180 MtrunA17_Chr6g0483491 30.526 380 244 6 10 375 6 379 6.58e-59 197
Msa0912180 MtrunA17_Chr7g0236841 30.000 380 246 6 10 375 6 379 6.87e-57 192
Msa0912180 MtrunA17_Chr3g0095031 31.622 370 236 5 18 373 94 460 4.62e-56 192
Msa0912180 MtrunA17_Chr6g0449911 32.603 365 227 9 12 361 163 523 1.82e-55 194
Msa0912180 MtrunA17_Chr4g0063551 31.343 402 229 7 3 361 119 516 2.54e-54 190
Msa0912180 MtrunA17_Chr7g0271121 35.277 343 199 9 39 363 131 468 5.91e-54 190
Msa0912180 MtrunA17_Chr4g0040761 30.811 370 244 5 15 373 20 388 8.53e-54 190
Msa0912180 MtrunA17_Chr4g0076491 29.574 399 249 5 10 377 283 680 1.01e-53 189
Msa0912180 MtrunA17_Chr1g0176041 30.357 392 239 6 12 373 152 539 2.01e-53 186
Msa0912180 MtrunA17_Chr1g0193491 32.665 349 211 7 18 347 118 461 4.66e-53 186
Msa0912180 MtrunA17_Chr6g0449741 28.822 399 250 6 10 377 245 640 4.32e-52 184
Msa0912180 MtrunA17_Chr2g0277821 32.514 366 233 5 10 361 500 865 2.00e-51 184
Msa0912180 MtrunA17_Chr1g0200181 30.691 391 246 8 12 380 143 530 4.33e-51 179
Msa0912180 MtrunA17_Chr1g0157691 29.923 391 249 8 12 380 145 532 1.97e-50 178
Msa0912180 MtrunA17_Chr4g0058591 33.333 321 205 3 5 316 190 510 4.98e-50 176
Msa0912180 MtrunA17_Chr2g0310581 29.679 374 246 4 11 370 50 420 1.36e-49 174
Msa0912180 MtrunA17_Chr5g0409981 31.579 361 227 6 7 349 97 455 1.97e-49 174
Msa0912180 MtrunA17_Chr7g0263771 30.769 364 242 4 24 379 143 504 9.87e-47 168
Msa0912180 MtrunA17_Chr3g0098121 30.728 371 239 6 14 369 152 519 4.99e-46 168
Msa0912180 MtrunA17_Chr6g0461751 28.610 374 233 8 12 361 138 501 1.57e-43 158
Msa0912180 MtrunA17_Chr2g0309551 25.653 421 248 7 14 369 109 529 7.87e-42 154
Msa0912180 MtrunA17_Chr2g0300291 29.688 384 250 8 3 369 129 509 1.21e-40 152
Msa0912180 MtrunA17_Chr2g0307471 26.902 368 252 8 8 359 85 451 1.27e-38 145
Msa0912180 MtrunA17_Chr3g0114081 28.312 385 240 9 1 350 1 384 7.43e-38 143
Msa0912180 MtrunA17_Chr4g0062301 28.532 361 228 11 26 363 109 462 1.77e-37 142
Msa0912180 MtrunA17_Chr6g0466611 27.532 385 239 10 12 363 140 517 1.70e-36 140
Msa0912180 MtrunA17_Chr8g0347151 28.967 397 225 12 15 374 202 578 2.84e-36 139
Msa0912180 MtrunA17_Chr8g0347161 27.342 395 240 10 15 377 103 482 7.72e-36 137
Msa0912180 MtrunA17_Chr8g0347171 26.718 393 245 10 15 377 161 540 3.28e-34 133
Msa0912180 MtrunA17_Chr8g0386451 25.616 406 243 12 7 374 281 665 5.16e-34 133
Msa0912180 MtrunA17_Chr6g0475251 27.806 392 241 10 14 372 103 485 3.41e-32 128
Msa0912180 MtrunA17_Chr4g0034411 27.200 375 247 9 12 362 66 438 5.08e-32 127
Msa0912180 MtrunA17_Chr5g0443991 30.267 337 212 10 33 350 95 427 1.76e-31 126
Msa0912180 MtrunA17_Chr1g0192021 25.061 411 243 10 14 364 26 431 2.49e-31 125
Msa0912180 MtrunA17_Chr1g0204391 26.617 402 247 9 20 377 50 447 7.71e-31 123
Msa0912180 MtrunA17_Chr7g0236831 24.816 407 226 9 24 350 173 579 4.11e-30 122
Msa0912180 MtrunA17_Chr4g0025141 32.850 207 118 4 187 372 14 220 6.56e-29 112
Msa0912180 MtrunA17_Chr3g0137901 36.585 164 96 3 220 376 10 172 4.89e-28 108
Msa0912180 MtrunA17_Chr3g0145781 25.128 390 253 9 11 361 129 518 3.29e-25 107
Msa0912180 MtrunA17_Chr4g0025131 34.969 163 103 2 11 170 53 215 3.47e-25 103
Msa0912180 MtrunA17_Chr8g0349951 35.252 139 89 1 10 147 6 144 2.47e-23 95.1
Msa0912180 MtrunA17_Chr4g0039471 28.426 197 138 1 14 207 223 419 4.55e-22 97.1
Msa0912180 MtrunA17_Chr4g0074521 26.902 368 212 15 20 343 88 442 7.24e-22 97.1
Msa0912180 MtrunA17_Chr5g0429221 21.149 383 282 7 12 380 1 377 7.79e-22 96.3
Msa0912180 MtrunA17_Chr3g0137891 34.969 163 101 4 52 210 2 163 8.69e-21 90.1
Msa0912180 MtrunA17_Chr4g0062321 27.619 210 147 4 116 321 25 233 8.80e-19 85.1
Msa0912180 MtrunA17_Chr4g0029191 27.511 229 139 6 13 214 43 271 9.74e-18 85.1
Msa0912180 MtrunA17_Chr4g0029191 34.831 89 52 1 282 364 391 479 4.62e-12 67.4
Msa0912180 MtrunA17_Chr2g0301281 26.695 236 146 6 6 214 33 268 1.06e-17 84.7
Msa0912180 MtrunA17_Chr2g0301281 34.831 89 52 1 282 364 388 476 2.42e-12 68.6
Msa0912180 MtrunA17_Chr4g0025101 28.947 228 92 7 171 369 7 193 2.60e-17 80.1
Msa0912180 MtrunA17_Chr0c30g0494091 26.442 208 132 5 20 213 88 288 4.65e-13 70.1
Msa0912180 MtrunA17_Chr1g0155201 24.803 254 163 9 133 372 13 252 1.79e-11 65.1
Msa0912180 MtrunA17_Chr2g0300871 22.647 340 217 11 29 342 36 355 5.10e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0912180 AT3G19760.1 83.594 384 57 1 3 380 25 408 0.0 682
Msa0912180 AT1G51380.1 70.052 384 106 2 3 380 12 392 0.0 570
Msa0912180 AT3G13920.3 63.003 373 132 1 14 380 30 402 2.08e-174 492
Msa0912180 AT3G13920.1 63.003 373 132 1 14 380 40 412 2.89e-174 492
Msa0912180 AT1G54270.1 62.735 373 133 1 14 380 40 412 7.24e-174 491
Msa0912180 AT1G72730.1 61.662 373 137 1 14 380 42 414 3.25e-170 481
Msa0912180 AT3G13920.4 61.930 373 131 2 14 380 40 407 3.60e-168 476
Msa0912180 AT1G54270.2 61.662 373 132 2 14 380 40 407 5.75e-168 476
Msa0912180 AT3G13920.5 64.103 351 120 1 14 358 44 394 3.98e-164 466
Msa0912180 AT3G13920.2 64.103 351 120 1 14 358 40 390 4.70e-164 466
Msa0912180 AT1G51380.2 72.624 263 72 0 3 265 12 274 1.78e-145 414
Msa0912180 AT1G51380.4 73.423 222 59 0 3 224 12 233 1.29e-119 348
Msa0912180 AT1G51380.3 73.423 222 59 0 3 224 12 233 2.30e-119 348
Msa0912180 AT4G00660.1 38.441 372 221 3 15 380 133 502 2.98e-87 273
Msa0912180 AT4G00660.2 38.441 372 221 3 15 380 133 502 2.98e-87 273
Msa0912180 AT3G61240.2 37.097 372 226 3 15 380 126 495 2.04e-84 265
Msa0912180 AT3G61240.1 37.097 372 226 3 15 380 126 495 2.04e-84 265
Msa0912180 AT2G45810.1 36.986 365 222 3 15 373 156 518 1.05e-81 259
Msa0912180 AT1G55150.1 34.605 367 222 6 10 361 96 459 7.59e-70 228
Msa0912180 AT1G55150.2 34.605 367 222 6 10 361 121 484 7.91e-70 228
Msa0912180 AT3G53110.1 33.850 387 231 8 11 372 89 475 1.11e-69 227
Msa0912180 AT5G11170.1 35.584 385 216 8 13 376 46 419 1.63e-69 225
Msa0912180 AT5G11200.1 35.584 385 216 8 13 376 46 419 1.63e-69 225
Msa0912180 AT5G63120.2 34.615 364 226 3 10 361 162 525 5.99e-67 222
Msa0912180 AT3G01540.1 35.789 380 224 7 12 372 157 535 1.06e-63 214
Msa0912180 AT3G01540.4 35.789 380 224 7 12 372 157 535 1.12e-63 214
Msa0912180 AT3G01540.2 35.789 380 224 7 12 372 157 535 1.12e-63 214
Msa0912180 AT3G01540.3 35.789 380 224 7 12 372 157 535 1.12e-63 214
Msa0912180 AT3G06480.1 34.555 382 226 7 14 374 436 814 1.27e-61 214
Msa0912180 AT5G14610.2 34.115 384 225 9 12 372 160 538 4.57e-61 208
Msa0912180 AT5G11200.3 32.160 426 216 9 13 376 46 460 5.13e-61 204
Msa0912180 AT5G14610.6 34.115 384 225 9 12 372 160 538 5.26e-61 207
Msa0912180 AT5G14610.5 34.115 384 225 9 12 372 168 546 6.05e-61 207
Msa0912180 AT3G22310.2 31.818 374 238 5 14 373 117 487 2.05e-59 202
Msa0912180 AT5G11170.2 35.569 343 189 8 52 373 2 333 2.11e-59 196
Msa0912180 AT3G22310.1 31.818 374 238 5 14 373 117 487 2.65e-59 202
Msa0912180 AT3G22330.1 31.948 385 223 7 14 373 105 475 3.93e-59 202
Msa0912180 AT2G47330.1 32.564 390 236 9 10 379 225 607 3.97e-58 202
Msa0912180 AT5G14610.7 33.696 368 216 9 28 372 45 407 8.19e-57 194
Msa0912180 AT5G14610.3 33.696 368 216 9 28 372 45 407 8.19e-57 194
Msa0912180 AT3G58510.1 32.242 397 241 8 4 373 140 535 1.77e-56 195
Msa0912180 AT3G58510.3 32.242 397 241 8 4 373 140 535 1.77e-56 195
Msa0912180 AT3G58510.2 32.242 397 241 8 4 373 140 535 1.77e-56 195
Msa0912180 AT5G26742.1 34.160 363 213 9 39 380 128 485 2.70e-56 196
Msa0912180 AT5G26742.2 34.160 363 213 9 39 380 128 485 2.73e-56 196
Msa0912180 AT2G33730.1 30.075 399 247 4 10 377 310 707 7.41e-56 195
Msa0912180 AT5G26742.3 34.078 358 210 9 44 380 40 392 1.63e-55 193
Msa0912180 AT5G14610.1 33.062 369 221 9 26 372 242 606 1.89e-55 194
Msa0912180 AT5G60990.1 30.711 394 240 7 4 373 3 387 2.77e-55 188
Msa0912180 AT1G77030.1 31.452 372 239 6 15 373 30 398 3.21e-55 194
Msa0912180 AT2G42520.2 31.472 394 232 8 12 373 158 545 4.66e-55 191
Msa0912180 AT2G42520.1 31.472 394 232 8 12 373 158 545 4.66e-55 191
Msa0912180 AT2G42520.3 31.472 394 232 8 12 373 158 545 4.66e-55 191
Msa0912180 AT3G58570.1 31.122 392 236 7 12 373 145 532 2.04e-53 187
Msa0912180 AT1G20920.2 31.880 367 234 6 10 361 188 553 1.72e-52 187
Msa0912180 AT5G11200.2 29.955 444 220 10 13 376 46 478 3.56e-52 181
Msa0912180 AT4G33370.1 31.282 390 243 8 12 379 96 482 7.93e-52 181
Msa0912180 AT5G63120.3 33.227 313 203 2 10 316 162 474 1.88e-51 179
Msa0912180 AT5G63120.4 33.227 313 203 2 10 316 162 474 1.88e-51 179
Msa0912180 AT5G63120.5 33.227 313 203 2 10 316 162 474 1.88e-51 179
Msa0912180 AT3G09720.2 32.249 369 240 4 19 379 147 513 2.06e-51 180
Msa0912180 AT3G09720.1 32.249 369 240 4 19 379 147 513 2.06e-51 180
Msa0912180 AT5G63120.1 33.227 313 203 2 10 316 162 474 2.12e-51 179
Msa0912180 AT1G20920.4 31.880 367 234 6 10 361 526 891 6.15e-51 183
Msa0912180 AT1G20920.6 31.880 367 234 6 10 361 526 891 6.15e-51 183
Msa0912180 AT1G20920.3 31.880 367 234 6 10 361 526 891 6.15e-51 183
Msa0912180 AT1G20920.7 31.880 367 234 6 10 361 526 891 6.15e-51 183
Msa0912180 AT1G20920.5 31.880 367 234 6 10 361 526 891 6.15e-51 183
Msa0912180 AT1G20920.1 31.880 367 234 6 10 361 526 891 6.15e-51 183
Msa0912180 AT1G16280.1 30.691 391 228 12 11 375 56 429 8.29e-51 177
Msa0912180 AT3G06480.2 33.750 320 201 5 14 325 436 752 3.88e-50 180
Msa0912180 AT5G51280.1 31.026 390 244 8 12 379 145 531 5.81e-50 177
Msa0912180 AT5G14610.4 33.333 318 201 7 12 321 168 482 2.14e-49 174
Msa0912180 AT3G02065.3 28.351 388 253 6 12 379 109 491 2.50e-48 171
Msa0912180 AT3G02065.2 28.351 388 253 6 12 379 109 491 2.50e-48 171
Msa0912180 AT1G31970.1 31.548 336 211 5 33 349 134 469 1.46e-46 167
Msa0912180 AT1G12770.2 26.969 419 232 8 14 363 111 524 7.01e-46 165
Msa0912180 AT1G12770.1 26.969 419 232 8 14 363 111 524 7.01e-46 165
Msa0912180 AT1G28180.1 31.751 337 210 6 43 363 3 335 2.65e-45 160
Msa0912180 AT4G16630.1 30.789 380 250 5 3 369 156 535 1.58e-44 164
Msa0912180 AT3G02065.1 28.492 358 231 6 42 379 2 354 1.19e-43 155
Msa0912180 AT3G09620.1 30.000 370 208 9 10 361 393 729 9.84e-43 159
Msa0912180 AT3G09620.2 30.000 370 208 9 10 361 394 730 9.85e-43 159
Msa0912180 AT5G63630.1 27.569 399 246 9 11 379 52 437 1.58e-41 153
Msa0912180 AT3G18600.1 28.610 367 247 7 7 359 83 448 4.23e-41 152
Msa0912180 AT5G05450.1 29.609 358 221 8 23 350 26 382 7.74e-41 152
Msa0912180 AT1G71370.1 29.670 364 231 9 20 359 23 385 1.57e-40 150
Msa0912180 AT5G63630.2 27.569 399 246 9 11 379 246 631 7.51e-40 150
Msa0912180 AT5G08620.1 28.293 410 243 12 4 379 71 463 6.49e-39 146
Msa0912180 AT5G08610.1 28.224 411 246 10 5 379 368 765 8.20e-39 148
Msa0912180 AT5G65900.1 27.945 365 249 5 9 359 150 514 1.04e-38 146
Msa0912180 AT5G62190.1 29.101 378 226 9 32 380 115 479 3.47e-36 140
Msa0912180 AT5G54910.1 29.301 372 237 13 12 363 70 435 9.12e-36 139
Msa0912180 AT1G63250.1 27.114 402 249 10 4 374 319 707 7.54e-34 133
Msa0912180 AT2G07750.1 26.108 406 248 11 4 374 366 754 1.08e-32 130
Msa0912180 AT4G09730.1 24.740 384 255 7 10 363 109 488 3.06e-32 128
Msa0912180 AT2G40700.1 25.328 458 255 13 8 378 25 482 5.53e-32 127
Msa0912180 AT4G34910.1 25.845 414 244 11 14 367 47 457 3.22e-30 122
Msa0912180 AT5G19210.2 26.684 386 232 13 20 362 83 460 1.85e-28 116
Msa0912180 AT1G71280.2 29.487 312 180 9 20 318 24 308 2.58e-28 113
Msa0912180 AT1G71280.1 29.260 311 182 9 20 318 24 308 1.58e-27 113
Msa0912180 AT3G06980.1 25.381 394 252 10 8 361 369 760 3.65e-25 107
Msa0912180 AT4G15850.1 23.294 425 223 13 14 356 45 448 1.96e-20 93.2
Msa0912180 AT3G16840.1 25.381 197 123 4 12 184 189 385 1.31e-14 75.9
Msa0912180 AT3G16840.2 25.381 197 123 4 12 184 190 386 1.35e-14 75.9

Find 89 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACGGCCAATTCGATTATT+TGG 0.156282 6_2:+20310674 None:intergenic
TCAATTGCCGTGTACAATTT+AGG 0.186773 6_2:+20313232 None:intergenic
TGCAATTATGGGAGAATTTC+GGG 0.192770 6_2:-20311621 Msa0912180:CDS
AATTGCATCTTTCGAGAATA+TGG 0.210189 6_2:-20313164 Msa0912180:CDS
AAGAGAAAGAGATGCAATTA+TGG 0.245902 6_2:-20311633 Msa0912180:CDS
ATTGGCCGTTCTGGACGTTT+TGG 0.252537 6_2:-20310662 Msa0912180:CDS
AATAATCGAATTGGCCGTTC+TGG 0.276634 6_2:-20310671 Msa0912180:CDS
ATGCAATTATGGGAGAATTT+CGG 0.295530 6_2:-20311622 Msa0912180:CDS
TACGTTGCCTCGAGAAATTT+TGG 0.334761 6_2:-20312122 Msa0912180:CDS
GAGTTTGTGACATGATTAAA+AGG 0.335963 6_2:-20312516 Msa0912180:CDS
ATATCTCCCTCCCGATCTTC+AGG 0.342306 6_2:-20312161 Msa0912180:CDS
TGCTGATAACAACAGATGTT+TGG 0.363013 6_2:-20311583 Msa0912180:CDS
ACGTCGCGGCCCTGGATGAT+AGG 0.373899 6_2:+20313061 None:intergenic
ATTGCATCTCTTTCTCTTTG+AGG 0.376417 6_2:+20311638 None:intergenic
GAGCAATGACGTCGCGGCCC+TGG 0.378821 6_2:+20313053 None:intergenic
TGTGTCTCCCACGCGGGAAC+TGG 0.380636 6_2:-20312680 Msa0912180:CDS
GTCATTGCTCAGGCTCAATC+TGG 0.384667 6_2:-20313040 Msa0912180:CDS
AGAGAAAGAGATGCAATTAT+GGG 0.391866 6_2:-20311632 Msa0912180:CDS
GACCTTCCAAATAATCGAAT+TGG 0.394156 6_2:-20310680 Msa0912180:CDS
ATAAGATCAGCAACATTCAT+TGG 0.404488 6_2:+20310432 None:intergenic
GAGAAAGTTATATCTGCTAT+TGG 0.414565 6_2:-20312646 Msa0912180:CDS
GCTGATAACAACAGATGTTT+GGG 0.425669 6_2:-20311582 Msa0912180:CDS
ATTGCATCTTTCGAGAATAT+GGG 0.433255 6_2:-20313163 Msa0912180:CDS
CCATCTAATTACAGGGCATA+AGG 0.449551 6_2:-20311959 Msa0912180:intron
TGCGATTGCACCTATCATCC+AGG 0.458327 6_2:-20313071 Msa0912180:CDS
AAAGTGTAGGCGAAGACATA+AGG 0.459571 6_2:-20312582 Msa0912180:CDS
CATGGAGCTCACGTAGTGTC+TGG 0.460432 6_2:-20312550 Msa0912180:CDS
GCAAACAGTGAGAGCAATCA+TGG 0.460588 6_2:+20313003 None:intergenic
ATCAAGACAACCTGAAGATC+GGG 0.462723 6_2:+20312151 None:intergenic
GCTCAGGCTCAATCTGGAAC+TGG 0.468328 6_2:-20313034 Msa0912180:CDS
CTGCGTGGAATCTATCAATA+CGG 0.476673 6_2:-20313124 Msa0912180:CDS
AGCTATCAAGTTGCTTGTGC+TGG 0.499944 6_2:-20312476 Msa0912180:intron
AATCAAGACAACCTGAAGAT+CGG 0.503982 6_2:+20312150 None:intergenic
GTACACGGCAATTGAAAGCT+TGG 0.507092 6_2:-20313224 Msa0912180:five_prime_UTR
AATTGAAAGCTTGGAGAAGA+TGG 0.511403 6_2:-20313215 Msa0912180:exon
GTCACCATGCATTGAGGAGA+CGG 0.516149 6_2:+20311664 None:intergenic
GGACGGAGGTATCGATAAGA+TGG 0.518084 6_2:+20312981 None:intergenic
CGAGCCCATGCTTGCATCGG+AGG 0.520742 6_2:-20312607 Msa0912180:CDS
TTTGCCTCCGATGCAAGCAT+GGG 0.521295 6_2:+20312603 None:intergenic
ACAACAGATGTTTGGGCTCG+TGG 0.521521 6_2:-20311575 Msa0912180:CDS
TCGTGGCCTTGATGTACAGC+AGG 0.521836 6_2:-20311558 Msa0912180:intron
GGCCAATTCGATTATTTGGA+AGG 0.529972 6_2:+20310678 None:intergenic
CACGTAGTGTCTGGAACACC+TGG 0.534536 6_2:-20312541 Msa0912180:CDS
TATAACTTTCTCAGTTTGTG+AGG 0.539616 6_2:+20312657 None:intergenic
ATCCCTAAATTGCGATCGAA+CGG 0.551080 6_2:-20313258 None:intergenic
TTGCCGTTGAAAGGGAGGAC+TGG 0.552225 6_2:-20311926 Msa0912180:CDS
AACTTGCCTGCTGTACATCA+AGG 0.558036 6_2:+20311552 None:intergenic
AAAGTTATATCTGCTATTGG+CGG 0.558436 6_2:-20312643 Msa0912180:CDS
ATCTGTTGTTATCAGCACTC+TGG 0.558524 6_2:+20311589 None:intergenic
TGATATTCTTACCATCACTC+AGG 0.562078 6_2:-20311877 Msa0912180:CDS
CTTCCAGTCCTCCCTTTCAA+CGG 0.566897 6_2:+20311923 None:intergenic
TTTCTGTAACACAAAGCGAA+AGG 0.571619 6_2:-20311847 Msa0912180:intron
TTTCGTTGCCGTTGAAAGGG+AGG 0.573539 6_2:-20311931 Msa0912180:CDS
GTCATCTCCAAAATTTCTCG+AGG 0.575420 6_2:+20312115 None:intergenic
AGGCATGTCACCATGCATTG+AGG 0.576582 6_2:+20311658 None:intergenic
GACATAAGGCAACTTGAACA+TGG 0.578733 6_2:-20312568 Msa0912180:CDS
ATGGGAGAATTTCGGGCTGG+AGG 0.586619 6_2:-20311614 Msa0912180:CDS
GAAAATAACAGCCTGAGTGA+TGG 0.589122 6_2:+20311866 None:intergenic
ATACGAGCCCATGCTTGCAT+CGG 0.590719 6_2:-20312610 Msa0912180:CDS
TGTGAGGCCAGTTCCCGCGT+GGG 0.594222 6_2:+20312673 None:intergenic
ATTATGGGAGAATTTCGGGC+TGG 0.595634 6_2:-20311617 Msa0912180:CDS
TTGCATCTTTCGAGAATATG+GGG 0.596713 6_2:-20313162 Msa0912180:CDS
TTGTGAGGCCAGTTCCCGCG+TGG 0.598017 6_2:+20312672 None:intergenic
ACGTGACGAATTAACGTTGG+AGG 0.598891 6_2:-20312053 Msa0912180:intron
GAAGATGGAGTTCGAGACAA+CGG 0.598987 6_2:-20313200 Msa0912180:exon
TTTAATTGTGTCTCCCACGC+GGG 0.610189 6_2:-20312686 Msa0912180:CDS
CTGATGAAATGCTTAGCAGA+GGG 0.618009 6_2:-20312213 Msa0912180:CDS
TTCACCGTCTCCTCAATGCA+TGG 0.622726 6_2:-20311668 Msa0912180:CDS
ATCATGTCACAAACTCGACC+AGG 0.628442 6_2:+20312523 None:intergenic
GCGATTGCACCTATCATCCA+GGG 0.630970 6_2:-20313070 Msa0912180:CDS
TTCGCCCAAAACGTCCAGAA+CGG 0.631007 6_2:+20310657 None:intergenic
AAAACGTGACGAATTAACGT+TGG 0.633814 6_2:-20312056 Msa0912180:CDS
AAGACAACCTGAAGATCGGG+AGG 0.633873 6_2:+20312154 None:intergenic
ATGTACTCACTCTCGGACGG+AGG 0.634898 6_2:+20312967 None:intergenic
TCTGATGAAATGCTTAGCAG+AGG 0.642998 6_2:-20312214 Msa0912180:CDS
AAGATGTACTCACTCTCGGA+CGG 0.644954 6_2:+20312964 None:intergenic
TGCATCGGAGGCAAAAGTGT+AGG 0.645004 6_2:-20312595 Msa0912180:CDS
CTTTAATTGTGTCTCCCACG+CGG 0.646289 6_2:-20312687 Msa0912180:CDS
GCACGCTGTTGAATCGCCGA+CGG 0.662677 6_2:+20313091 None:intergenic
GAGCCTGAGCAATGACGTCG+CGG 0.666235 6_2:+20313047 None:intergenic
ATGGAGTTCGAGACAACGGA+GGG 0.672934 6_2:-20313196 Msa0912180:CDS
GATGGAGTTCGAGACAACGG+AGG 0.683107 6_2:-20313197 Msa0912180:CDS
AATTTCTCGAGGCAACGTAG+CGG 0.683757 6_2:+20312126 None:intergenic
ATACGGTTTCGAGAATCCGT+CGG 0.683964 6_2:-20313107 Msa0912180:CDS
ACGGAAACCTAAATTGTACA+CGG 0.685249 6_2:-20313239 Msa0912180:five_prime_UTR
ATAAAAGACGATTTACTGCG+TGG 0.693059 6_2:-20313139 Msa0912180:CDS
AGACAACCTGAAGATCGGGA+GGG 0.702108 6_2:+20312155 None:intergenic
AGTTTATGACAGATCCCGTG+AGG 0.729356 6_2:-20312087 Msa0912180:CDS
AACAGTGAGAGCAATCATGG+AGG 0.739002 6_2:+20313006 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATAATTTCAGTTTTAATT+AGG - chr6_2:20310856-20310875 Msa0912180:intron 10.0%
!!! TATAATTTCAGTTTTAATTA+GGG - chr6_2:20310857-20310876 Msa0912180:intron 10.0%
!! AAGGTAAATGAAAATTATAA+AGG - chr6_2:20311845-20311864 Msa0912180:intron 15.0%
!!! CTATATTAAAATTGATTTTG+AGG + chr6_2:20312509-20312528 None:intergenic 15.0%
!! ATTGCTTATTACTTATTATT+TGG - chr6_2:20313113-20313132 Msa0912180:CDS 15.0%
!!! GTTTTAATTAGGGTTTTTAA+AGG - chr6_2:20310766-20310785 Msa0912180:intron 20.0%
!!! TGTAATTTTAGGTTGAATTA+GGG - chr6_2:20310804-20310823 Msa0912180:intron 20.0%
!!! GTTTTAATTAGGGTTTTTAA+AGG - chr6_2:20310766-20310785 Msa0912180:intron 20.0%
!!! TGTAATTTTAGGTTGAATTA+GGG - chr6_2:20310804-20310823 Msa0912180:intron 20.0%
!! AAAGAGAAAAAACAATGAAT+AGG + chr6_2:20311235-20311254 None:intergenic 20.0%
!! AAATTAAGAGATTAATGAGT+TGG + chr6_2:20311319-20311338 None:intergenic 20.0%
!!! GAATTATGAATATGTGTTTT+CGG - chr6_2:20312266-20312285 Msa0912180:intron 20.0%
!!! AATTTACACCAATTTTTACA+TGG - chr6_2:20312440-20312459 Msa0912180:intron 20.0%
!!! TACTAAAGATTGTTAGTTTA+AGG + chr6_2:20312684-20312703 None:intergenic 20.0%
!! AAAAAAATCAATGAATTGCA+GGG + chr6_2:20312893-20312912 None:intergenic 20.0%
!! TAGCAGTATAGTGTAATTTT+AGG - chr6_2:20310793-20310812 Msa0912180:intron 25.0%
!!! GTGTAATTTTAGGTTGAATT+AGG - chr6_2:20310803-20310822 Msa0912180:intron 25.0%
!! TAGCAGTATAGTGTAATTTT+AGG - chr6_2:20310793-20310812 Msa0912180:intron 25.0%
!!! GTGTAATTTTAGGTTGAATT+AGG - chr6_2:20310803-20310822 Msa0912180:intron 25.0%
!! TTTCTCTTTCTTTTTCTTGT+CGG - chr6_2:20311246-20311265 Msa0912180:intron 25.0%
! ATATGGTTTATGATGCATTA+TGG + chr6_2:20311290-20311309 None:intergenic 25.0%
! AATGCATCATAAACCATATT+TGG - chr6_2:20311291-20311310 Msa0912180:intron 25.0%
!! GTTTCTCTTATAGTTTTTCT+AGG - chr6_2:20311348-20311367 Msa0912180:intron 25.0%
! AAATATATCATAGAGCACAA+AGG + chr6_2:20311670-20311689 None:intergenic 25.0%
! GAAAATTATAAAGGACTTGT+AGG - chr6_2:20311854-20311873 Msa0912180:CDS 25.0%
!! TTTATATGTCCTTTTCAAAC+AGG - chr6_2:20311947-20311966 Msa0912180:CDS 25.0%
! ATTCACATAGCTTAAATATC+TGG - chr6_2:20312229-20312248 Msa0912180:intron 25.0%
!! TATAATTCCGTCTATGTTTT+GGG + chr6_2:20312579-20312598 None:intergenic 25.0%
! TATACATAAAAACGCAACAA+TGG + chr6_2:20312770-20312789 None:intergenic 25.0%
! CAAAAAAATCAATGAATTGC+AGG + chr6_2:20312894-20312913 None:intergenic 25.0%
!!! CTGCAATTCATTGATTTTTT+TGG - chr6_2:20312892-20312911 Msa0912180:intron 25.0%
!!! TGATTTTTTTGGATTGTAGA+CGG - chr6_2:20312903-20312922 Msa0912180:intron 25.0%
AATTGCATCTTTCGAGAATA+TGG - chr6_2:20310509-20310528 Msa0912180:CDS 30.0%
ATTGCATCTTTCGAGAATAT+GGG - chr6_2:20310510-20310529 Msa0912180:CDS 30.0%
TATAACTTTCTCAGTTTGTG+AGG + chr6_2:20311019-20311038 None:intergenic 30.0%
GAGAAAGTTATATCTGCTAT+TGG - chr6_2:20311027-20311046 Msa0912180:intron 30.0%
AAAGTTATATCTGCTATTGG+CGG - chr6_2:20311030-20311049 Msa0912180:intron 30.0%
GAGTTTGTGACATGATTAAA+AGG - chr6_2:20311157-20311176 Msa0912180:intron 30.0%
! TAATGAGTTGGATCCAAATA+TGG + chr6_2:20311307-20311326 None:intergenic 30.0%
! TTAATTCGTCACGTTTTACA+AGG + chr6_2:20311614-20311633 None:intergenic 30.0%
GTGCTCTATGATATATTTAC+CGG - chr6_2:20311672-20311691 Msa0912180:CDS 30.0%
AGGATAACAACATCAATATC+CGG + chr6_2:20311694-20311713 None:intergenic 30.0%
TTATCCTCCATCTAATTACA+GGG - chr6_2:20311707-20311726 Msa0912180:CDS 30.0%
TTCTGTACTCTATTTACACA+TGG - chr6_2:20311889-20311908 Msa0912180:CDS 30.0%
AAGAGAAAGAGATGCAATTA+TGG - chr6_2:20312040-20312059 Msa0912180:intron 30.0%
AGAGAAAGAGATGCAATTAT+GGG - chr6_2:20312041-20312060 Msa0912180:intron 30.0%
ATGCAATTATGGGAGAATTT+CGG - chr6_2:20312051-20312070 Msa0912180:intron 30.0%
!! TTATTTGTGCCTCTAAAACA+TGG - chr6_2:20312203-20312222 Msa0912180:CDS 30.0%
GACAAGTTCCATGTAAAAAT+TGG + chr6_2:20312451-20312470 None:intergenic 30.0%
GAGGCAAAGTTATTTATGTT+AGG + chr6_2:20312490-20312509 None:intergenic 30.0%
! CTATAATTCCGTCTATGTTT+TGG + chr6_2:20312580-20312599 None:intergenic 30.0%
TATTCTTATTACATCCAGCA+AGG - chr6_2:20312638-20312657 Msa0912180:CDS 30.0%
! CTAATGTCGTAACATGTTTT+CGG - chr6_2:20312792-20312811 Msa0912180:intron 30.0%
CTTATTATTTGGCCTAATCA+TGG - chr6_2:20313124-20313143 Msa0912180:CDS 30.0%
ATAAGATCAGCAACATTCAT+TGG + chr6_2:20313244-20313263 None:intergenic 30.0%
ACGGAAACCTAAATTGTACA+CGG - chr6_2:20310434-20310453 Msa0912180:CDS 35.0%
TCAATTGCCGTGTACAATTT+AGG + chr6_2:20310444-20310463 None:intergenic 35.0%
AATTGAAAGCTTGGAGAAGA+TGG - chr6_2:20310458-20310477 Msa0912180:CDS 35.0%
TTGCATCTTTCGAGAATATG+GGG - chr6_2:20310511-20310530 Msa0912180:CDS 35.0%
ATAAAAGACGATTTACTGCG+TGG - chr6_2:20310534-20310553 Msa0912180:intron 35.0%
AAACAAGATGTACTCACTCT+CGG + chr6_2:20310716-20310735 None:intergenic 35.0%
! AGGGTTTCTCATGTTTTGTA+TGG - chr6_2:20310924-20310943 Msa0912180:intron 35.0%
! GGGTTTCTCATGTTTTGTAT+GGG - chr6_2:20310925-20310944 Msa0912180:intron 35.0%
GCACACTGTACATACAAAAT+TGG + chr6_2:20310963-20310982 None:intergenic 35.0%
! ATTTTGTATGTACAGTGTGC+AGG - chr6_2:20310963-20310982 Msa0912180:intron 35.0%
CTTGACACATGATTAGATGA+AGG + chr6_2:20311403-20311422 None:intergenic 35.0%
CTTCATCTAATCATGTGTCA+AGG - chr6_2:20311401-20311420 Msa0912180:intron 35.0%
AATCAAGACAACCTGAAGAT+CGG + chr6_2:20311526-20311545 None:intergenic 35.0%
AAAACGTGACGAATTAACGT+TGG - chr6_2:20311617-20311636 Msa0912180:CDS 35.0%
GTTATCCTCCATCTAATTAC+AGG - chr6_2:20311706-20311725 Msa0912180:CDS 35.0%
! AATTTTTCGTTGCCGTTGAA+AGG - chr6_2:20311738-20311757 Msa0912180:intron 35.0%
! ATTTTTCGTTGCCGTTGAAA+GGG - chr6_2:20311739-20311758 Msa0912180:intron 35.0%
TGATATTCTTACCATCACTC+AGG - chr6_2:20311796-20311815 Msa0912180:intron 35.0%
TTTCTGTAACACAAAGCGAA+AGG - chr6_2:20311826-20311845 Msa0912180:intron 35.0%
! ATATGTCCTTTTCAAACAGG+TGG - chr6_2:20311950-20311969 Msa0912180:intron 35.0%
ATTGCATCTCTTTCTCTTTG+AGG + chr6_2:20312038-20312057 None:intergenic 35.0%
TGCAATTATGGGAGAATTTC+GGG - chr6_2:20312052-20312071 Msa0912180:intron 35.0%
TGCTGATAACAACAGATGTT+TGG - chr6_2:20312090-20312109 Msa0912180:CDS 35.0%
GCTGATAACAACAGATGTTT+GGG - chr6_2:20312091-20312110 Msa0912180:CDS 35.0%
ATTCTTACCTTCCTTACACA+AGG - chr6_2:20312155-20312174 Msa0912180:CDS 35.0%
! AGTAAAGAAAGCCTTGTGTA+AGG + chr6_2:20312169-20312188 None:intergenic 35.0%
!! GTGAATTCACCATGTTTTAG+AGG + chr6_2:20312215-20312234 None:intergenic 35.0%
TTGCTGACATGAACAAATGT+AGG - chr6_2:20312607-20312626 Msa0912180:CDS 35.0%
TAGTTTAAGGAGTTGTGAAC+TGG + chr6_2:20312671-20312690 None:intergenic 35.0%
TTTCAAATGTGACTACTCAG+TGG + chr6_2:20312857-20312876 None:intergenic 35.0%
!!! TTTTTTTGGATTGTAGACGG+AGG - chr6_2:20312906-20312925 Msa0912180:intron 35.0%
GACCTTCCAAATAATCGAAT+TGG - chr6_2:20312993-20313012 Msa0912180:CDS 35.0%
CTGCGTGGAATCTATCAATA+CGG - chr6_2:20310549-20310568 Msa0912180:intron 40.0%
AAAGTGTAGGCGAAGACATA+AGG - chr6_2:20311091-20311110 Msa0912180:intron 40.0%
GACATAAGGCAACTTGAACA+TGG - chr6_2:20311105-20311124 Msa0912180:intron 40.0%
AAGGATAGTCAGATCACAAG+AGG + chr6_2:20311384-20311403 None:intergenic 40.0%
TCTGATGAAATGCTTAGCAG+AGG - chr6_2:20311459-20311478 Msa0912180:intron 40.0%
CTGATGAAATGCTTAGCAGA+GGG - chr6_2:20311460-20311479 Msa0912180:intron 40.0%
ATCAAGACAACCTGAAGATC+GGG + chr6_2:20311525-20311544 None:intergenic 40.0%
! TACGTTGCCTCGAGAAATTT+TGG - chr6_2:20311551-20311570 Msa0912180:intron 40.0%
GTCATCTCCAAAATTTCTCG+AGG + chr6_2:20311561-20311580 None:intergenic 40.0%
! ACGTTTTACAAGGATCCTCA+CGG + chr6_2:20311604-20311623 None:intergenic 40.0%
TATGCCCTGTAATTAGATGG+AGG + chr6_2:20311714-20311733 None:intergenic 40.0%
CCTTATGCCCTGTAATTAGA+TGG + chr6_2:20311717-20311736 None:intergenic 40.0%
CCATCTAATTACAGGGCATA+AGG - chr6_2:20311714-20311733 Msa0912180:intron 40.0%
GAAAATAACAGCCTGAGTGA+TGG + chr6_2:20311810-20311829 None:intergenic 40.0%
CTCTATTTACACATGGCGAT+TGG - chr6_2:20311896-20311915 Msa0912180:CDS 40.0%
! TCCTTTTCAAACAGGTGGAT+TGG - chr6_2:20311955-20311974 Msa0912180:intron 40.0%
ACCAATCCACCTGTTTGAAA+AGG + chr6_2:20311959-20311978 None:intergenic 40.0%
ATCTGTTGTTATCAGCACTC+TGG + chr6_2:20312087-20312106 None:intergenic 40.0%
! AAGAAAGCCTTGTGTAAGGA+AGG + chr6_2:20312165-20312184 None:intergenic 40.0%
!! TAGAGACTAGCTTCTGCATT+AGG - chr6_2:20312411-20312430 Msa0912180:intron 40.0%
! GTGCGTTTGTTTCTCTTATC+AGG - chr6_2:20312950-20312969 Msa0912180:intron 40.0%
GGCCAATTCGATTATTTGGA+AGG + chr6_2:20312998-20313017 None:intergenic 40.0%
GAACGGCCAATTCGATTATT+TGG + chr6_2:20313002-20313021 None:intergenic 40.0%
AATAATCGAATTGGCCGTTC+TGG - chr6_2:20313002-20313021 Msa0912180:CDS 40.0%
GTACACGGCAATTGAAAGCT+TGG - chr6_2:20310449-20310468 Msa0912180:CDS 45.0%
GAAGATGGAGTTCGAGACAA+CGG - chr6_2:20310473-20310492 Msa0912180:CDS 45.0%
ATACGGTTTCGAGAATCCGT+CGG - chr6_2:20310566-20310585 Msa0912180:intron 45.0%
AACAGTGAGAGCAATCATGG+AGG + chr6_2:20310670-20310689 None:intergenic 45.0%
GCAAACAGTGAGAGCAATCA+TGG + chr6_2:20310673-20310692 None:intergenic 45.0%
AAGATGTACTCACTCTCGGA+CGG + chr6_2:20310712-20310731 None:intergenic 45.0%
CTTTAATTGTGTCTCCCACG+CGG - chr6_2:20310986-20311005 Msa0912180:intron 45.0%
TTTAATTGTGTCTCCCACGC+GGG - chr6_2:20310987-20311006 Msa0912180:intron 45.0%
ATCATGTCACAAACTCGACC+AGG + chr6_2:20311153-20311172 None:intergenic 45.0%
! AGCTATCAAGTTGCTTGTGC+TGG - chr6_2:20311197-20311216 Msa0912180:intron 45.0%
AGTCAGATCACAAGAGGTAG+AGG + chr6_2:20311378-20311397 None:intergenic 45.0%
AATTTCTCGAGGCAACGTAG+CGG + chr6_2:20311550-20311569 None:intergenic 45.0%
AGTTTATGACAGATCCCGTG+AGG - chr6_2:20311586-20311605 Msa0912180:CDS 45.0%
! CGTTTTACAAGGATCCTCAC+GGG + chr6_2:20311603-20311622 None:intergenic 45.0%
ACGTGACGAATTAACGTTGG+AGG - chr6_2:20311620-20311639 Msa0912180:CDS 45.0%
ATTATGGGAGAATTTCGGGC+TGG - chr6_2:20312056-20312075 Msa0912180:CDS 45.0%
AACTTGCCTGCTGTACATCA+AGG + chr6_2:20312124-20312143 None:intergenic 45.0%
ACCCTCACCCAAAACATAGA+CGG - chr6_2:20312569-20312588 Msa0912180:CDS 45.0%
!! TCCGTCTATGTTTTGGGTGA+GGG + chr6_2:20312573-20312592 None:intergenic 45.0%
!! TTCCGTCTATGTTTTGGGTG+AGG + chr6_2:20312574-20312593 None:intergenic 45.0%
GAACTGGTGATATTCCTTGC+TGG + chr6_2:20312655-20312674 None:intergenic 45.0%
GTGACTACTCAGTGGTCATA+AGG + chr6_2:20312849-20312868 None:intergenic 45.0%
! TTGGCCTAATCATGGTTTGC+AGG - chr6_2:20313132-20313151 Msa0912180:CDS 45.0%
! TGGCCTAATCATGGTTTGCA+GGG - chr6_2:20313133-20313152 Msa0912180:CDS 45.0%
!!! ATATAATTTTAGTTTTAATT+AGG - chr6_2:20310755-20310774 Msa0912180:intron 5.0%
!!! TATAATTTTAGTTTTAATTA+GGG - chr6_2:20310756-20310775 Msa0912180:intron 5.0%
!!! TAAATTTTTAATTTTATTAC+AGG - chr6_2:20311431-20311450 Msa0912180:intron 5.0%
ATGGAGTTCGAGACAACGGA+GGG - chr6_2:20310477-20310496 Msa0912180:CDS 50.0%
TGCGATTGCACCTATCATCC+AGG - chr6_2:20310602-20310621 Msa0912180:intron 50.0%
GCGATTGCACCTATCATCCA+GGG - chr6_2:20310603-20310622 Msa0912180:intron 50.0%
GTCATTGCTCAGGCTCAATC+TGG - chr6_2:20310633-20310652 Msa0912180:intron 50.0%
!! GGACGGAGGTATCGATAAGA+TGG + chr6_2:20310695-20310714 None:intergenic 50.0%
ATACGAGCCCATGCTTGCAT+CGG - chr6_2:20311063-20311082 Msa0912180:intron 50.0%
TTTGCCTCCGATGCAAGCAT+GGG + chr6_2:20311073-20311092 None:intergenic 50.0%
! TTTTGCCTCCGATGCAAGCA+TGG + chr6_2:20311074-20311093 None:intergenic 50.0%
! TGCATCGGAGGCAAAAGTGT+AGG - chr6_2:20311078-20311097 Msa0912180:intron 50.0%
ATATCTCCCTCCCGATCTTC+AGG - chr6_2:20311512-20311531 Msa0912180:intron 50.0%
AGACAACCTGAAGATCGGGA+GGG + chr6_2:20311521-20311540 None:intergenic 50.0%
AAGACAACCTGAAGATCGGG+AGG + chr6_2:20311522-20311541 None:intergenic 50.0%
TTTCGTTGCCGTTGAAAGGG+AGG - chr6_2:20311742-20311761 Msa0912180:intron 50.0%
CTTCCAGTCCTCCCTTTCAA+CGG + chr6_2:20311753-20311772 None:intergenic 50.0%
TTCACCGTCTCCTCAATGCA+TGG - chr6_2:20312005-20312024 Msa0912180:intron 50.0%
GTCACCATGCATTGAGGAGA+CGG + chr6_2:20312012-20312031 None:intergenic 50.0%
AGGCATGTCACCATGCATTG+AGG + chr6_2:20312018-20312037 None:intergenic 50.0%
ACAACAGATGTTTGGGCTCG+TGG - chr6_2:20312098-20312117 Msa0912180:CDS 50.0%
GGGTGAGGGTTTGACAAATG+CGG + chr6_2:20312559-20312578 None:intergenic 50.0%
!! ATTGGCCGTTCTGGACGTTT+TGG - chr6_2:20313011-20313030 Msa0912180:CDS 50.0%
!! TTGGCCGTTCTGGACGTTTT+GGG - chr6_2:20313012-20313031 Msa0912180:CDS 50.0%
TTCGCCCAAAACGTCCAGAA+CGG + chr6_2:20313019-20313038 None:intergenic 50.0%
!! TTCTGGACGTTTTGGGCGAA+AGG - chr6_2:20313019-20313038 Msa0912180:CDS 50.0%
!! ACGTTTTGGGCGAAAGGTAC+TGG - chr6_2:20313025-20313044 Msa0912180:CDS 50.0%
ACGCCCTGCAAACCATGATT+AGG + chr6_2:20313139-20313158 None:intergenic 50.0%
GATGGAGTTCGAGACAACGG+AGG - chr6_2:20310476-20310495 Msa0912180:CDS 55.0%
! GCTCAGGCTCAATCTGGAAC+TGG - chr6_2:20310639-20310658 Msa0912180:intron 55.0%
ATGTACTCACTCTCGGACGG+AGG + chr6_2:20310709-20310728 None:intergenic 55.0%
CATGGAGCTCACGTAGTGTC+TGG - chr6_2:20311123-20311142 Msa0912180:intron 55.0%
! CACGTAGTGTCTGGAACACC+TGG - chr6_2:20311132-20311151 Msa0912180:intron 55.0%
TTGCCGTTGAAAGGGAGGAC+TGG - chr6_2:20311747-20311766 Msa0912180:intron 55.0%
! ATGGGAGAATTTCGGGCTGG+AGG - chr6_2:20312059-20312078 Msa0912180:CDS 55.0%
! TCGTGGCCTTGATGTACAGC+AGG - chr6_2:20312115-20312134 Msa0912180:CDS 55.0%
GCACGCTGTTGAATCGCCGA+CGG + chr6_2:20310585-20310604 None:intergenic 60.0%
GAGCCTGAGCAATGACGTCG+CGG + chr6_2:20310629-20310648 None:intergenic 60.0%
ACGTCGCGGCCCTGGATGAT+AGG + chr6_2:20310615-20310634 None:intergenic 65.0%
TGTGTCTCCCACGCGGGAAC+TGG - chr6_2:20310993-20311012 Msa0912180:intron 65.0%
TGTGAGGCCAGTTCCCGCGT+GGG + chr6_2:20311003-20311022 None:intergenic 65.0%
TTGTGAGGCCAGTTCCCGCG+TGG + chr6_2:20311004-20311023 None:intergenic 65.0%
CGAGCCCATGCTTGCATCGG+AGG - chr6_2:20311066-20311085 Msa0912180:intron 65.0%
GAGCAATGACGTCGCGGCCC+TGG + chr6_2:20310623-20310642 None:intergenic 70.0%
GGGCCGCGACGTCATTGCTC+AGG - chr6_2:20310623-20310642 Msa0912180:intron 70.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 20310428 20313267 20310428 ID=Msa0912180;Name=Msa0912180
chr6_2 mRNA 20310428 20313267 20310428 ID=Msa0912180-mRNA-1;Parent=Msa0912180;Name=Msa0912180-mRNA-1;_AED=0.09;_eAED=0.09;_QI=49|0.66|0.57|1|1|1|7|0|380
chr6_2 exon 20310428 20310541 20310428 ID=Msa0912180-mRNA-1:exon:4127;Parent=Msa0912180-mRNA-1
chr6_2 exon 20310655 20310723 20310655 ID=Msa0912180-mRNA-1:exon:4126;Parent=Msa0912180-mRNA-1
chr6_2 exon 20311559 20311726 20311559 ID=Msa0912180-mRNA-1:exon:4125;Parent=Msa0912180-mRNA-1
chr6_2 exon 20311848 20311967 20311848 ID=Msa0912180-mRNA-1:exon:4124;Parent=Msa0912180-mRNA-1
chr6_2 exon 20312054 20312242 20312054 ID=Msa0912180-mRNA-1:exon:4123;Parent=Msa0912180-mRNA-1
chr6_2 exon 20312477 20312717 20312477 ID=Msa0912180-mRNA-1:exon:4122;Parent=Msa0912180-mRNA-1
chr6_2 exon 20312977 20313267 20312977 ID=Msa0912180-mRNA-1:exon:4121;Parent=Msa0912180-mRNA-1
chr6_2 five_prime_UTR 20313219 20313267 20313219 ID=Msa0912180-mRNA-1:five_prime_utr;Parent=Msa0912180-mRNA-1
chr6_2 CDS 20312977 20313218 20312977 ID=Msa0912180-mRNA-1:cds;Parent=Msa0912180-mRNA-1
chr6_2 CDS 20312477 20312717 20312477 ID=Msa0912180-mRNA-1:cds;Parent=Msa0912180-mRNA-1
chr6_2 CDS 20312054 20312242 20312054 ID=Msa0912180-mRNA-1:cds;Parent=Msa0912180-mRNA-1
chr6_2 CDS 20311848 20311967 20311848 ID=Msa0912180-mRNA-1:cds;Parent=Msa0912180-mRNA-1
chr6_2 CDS 20311559 20311726 20311559 ID=Msa0912180-mRNA-1:cds;Parent=Msa0912180-mRNA-1
chr6_2 CDS 20310655 20310723 20310655 ID=Msa0912180-mRNA-1:cds;Parent=Msa0912180-mRNA-1
chr6_2 CDS 20310428 20310541 20310428 ID=Msa0912180-mRNA-1:cds;Parent=Msa0912180-mRNA-1
Gene Sequence

>Msa0912180

ATGGAGTTCGAGACAACGGAGGGTGTTAAGACAATTGCATCTTTCGAGAATATGGGGATAAAAGACGATTTACTGCGTGGAATCTATCAATACGGTTTCGAGAATCCGTCGGCGATTCAACAGCGTGCGATTGCACCTATCATCCAGGGCCGCGACGTCATTGCTCAGGCTCAATCTGGAACTGGAAAGACCTCCATGATTGCTCTCACTGTTTGCCATCTTATCGATACCTCCGTCCGAGATGTGCAGGCTTTAATTGTGTCTCCCACGCGGGAACTGGCCTCACAAACTGAGAAAGTTATATCTGCTATTGGCGGTTTCACAAATATACGAGCCCATGCTTGCATCGGAGGCAAAAGTGTAGGCGAAGACATAAGGCAACTTGAACATGGAGCTCACGTAGTGTCTGGAACACCTGGTCGAGTTTGTGACATGATTAAAAGGAGAACATTGCGAACAAGAGCTATCAAGTTGCTTGTGCTGGATGAATCTGATGAAATGCTTAGCAGAGGGTTCAAAGATCAAATATATGATGTGTATAGATATCTCCCTCCCGATCTTCAGGTTGTCTTGATTTCCGCTACGTTGCCTCGAGAAATTTTGGAGATGACAAATAAGTTTATGACAGATCCCGTGAGGATCCTTGTAAAACGTGACGAATTAACGTTGGAGGGCATAAGGCAATTTTTCGTTGCCGTTGAAAGGGAGGACTGGAAGTTCGACACTCTGTGCGATCTTTATGATATTCTTACCATCACTCAGGCTGTTATTTTCTGTAACACAAAGCGAAAGGTGGATTGGTTAACTGAAAAGTTGCGTAACAATAATTTCACCGTCTCCTCAATGCATGGTGACATGCCTCAAAGAGAAAGAGATGCAATTATGGGAGAATTTCGGGCTGGAGGAACCAGAGTGCTGATAACAACAGATGTTTGGGCTCGTGGCCTTGATGTACAGCAGGTTTCTCTAGTTATTAATTACGACCTTCCAAATAATCGAATTGGCCGTTCTGGACGTTTTGGGCGAAAGGGCGTTGCAATAAATTTTGTGAAAAGCGATGATATTAAGATATTGAGAGATATTGAGCAATATTACAGTACTCAAATTGATGATCTGCCAATGAATGTTGCTGATCTTATATAA
Protein sequence

>Msa0912180

MEFETTEGVKTIASFENMGIKDDLLRGIYQYGFENPSAIQQRAIAPIIQGRDVIAQAQSGTGKTSMIALTVCHLIDTSVRDVQALIVSPTRELASQTEKVISAIGGFTNIRAHACIGGKSVGEDIRQLEHGAHVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVVLISATLPREILEMTNKFMTDPVRILVKRDELTLEGIRQFFVAVEREDWKFDTLCDLYDILTITQAVIFCNTKRKVDWLTEKLRNNNFTVSSMHGDMPQRERDAIMGEFRAGGTRVLITTDVWARGLDVQQVSLVINYDLPNNRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDDLPMNVADLI*