AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0914210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0914210 MtrunA17_Chr7g0271761 53.226 124 36 1 30 153 30 131 1.77e-30 109
Msa0914210 MtrunA17_Chr2g0294701 52.419 124 37 1 30 153 30 131 4.33e-30 108
Msa0914210 MtrunA17_Chr4g0068171 50.806 124 39 1 30 153 30 131 3.20e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0914210 AT4G02080.1 52.419 124 37 1 30 153 30 131 5.72e-30 108
Msa0914210 AT3G62560.1 52.419 124 37 1 30 153 30 131 6.30e-30 108
Msa0914210 AT1G56330.1 50.806 124 39 1 30 153 30 131 2.78e-29 107
Msa0914210 AT1G09180.1 47.581 124 43 1 30 153 30 131 2.67e-26 99.4
Msa0914210 AT1G02620.1 69.388 49 15 0 105 153 12 60 1.62e-14 66.6

Find 77 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCAACCTCATCTTTC+TGG 0.109259 6_2:+27219705 None:intergenic
TCCCTTTCCGCTCATGAAAT+TGG 0.172813 6_2:-27219764 Msa0914210:five_prime_UTR
CAGAAGGTCAAGGATGAAAT+TGG 0.206313 6_2:-27219658 Msa0914210:CDS
TCTCGCTGCGCCACTGTTTC+CGG 0.273332 6_2:-27219921 Msa0914210:five_prime_UTR
GTGTAGTATTGTGAAGAAAA+TGG 0.299445 6_2:-27217569 Msa0914210:three_prime_UTR
CCCTTTCCGCTCATGAAATT+GGG 0.328424 6_2:-27219763 Msa0914210:five_prime_UTR
AGACGAATTGCGTTACCATT+TGG 0.332275 6_2:-27217668 Msa0914210:CDS
GATATTTGACTCTTATGTTA+TGG 0.333300 6_2:+27219987 None:intergenic
TTTAGCTTTGGGCTATGAAA+TGG 0.341745 6_2:+27219958 None:intergenic
GACGAATTGCGTTACCATTT+GGG 0.348316 6_2:-27217667 Msa0914210:CDS
ATCAGATTGCTCGTAGAGTA+TGG 0.358368 6_2:-27218624 Msa0914210:CDS
CCCAATTTCATGAGCGGAAA+GGG 0.382770 6_2:+27219763 None:intergenic
TAGTCTCTGTACTTATGAAT+TGG 0.389228 6_2:-27217458 Msa0914210:three_prime_UTR
AATGTCCCTTTCCTTGTCCT+TGG 0.407599 6_2:-27217724 Msa0914210:CDS
ACGGCAACGAAGTTTAGCTT+TGG 0.411869 6_2:+27219946 None:intergenic
ATATCTTTATCTCTGAGTCT+TGG 0.420271 6_2:-27218776 Msa0914210:CDS
CATCAGAAGAGTTGAGTATT+GGG 0.422638 6_2:-27218682 Msa0914210:CDS
TCGTCTTCTGAGGCAACATA+TGG 0.429959 6_2:+27217685 None:intergenic
TTCTTTAGTAAGAGATGGCT+TGG 0.431594 6_2:-27217500 Msa0914210:three_prime_UTR
TTAGCTTTGGGCTATGAAAT+GGG 0.435894 6_2:+27219959 None:intergenic
CGGCAACGAAGTTTAGCTTT+GGG 0.438285 6_2:+27219947 None:intergenic
GGCTTGAGCAACTTCGCCAC+TGG 0.454627 6_2:-27217646 Msa0914210:exon
GTTATGGTGATGGTTTCAAA+TGG 0.456424 6_2:-27217546 Msa0914210:three_prime_UTR
TGTAGTATTGTGAAGAAAAT+GGG 0.463302 6_2:-27217568 Msa0914210:three_prime_UTR
CGAAGTTGCTCAAGCCCAAA+TGG 0.477033 6_2:+27217653 None:intergenic
TTATGGTGATGGTTTCAAAT+GGG 0.483238 6_2:-27217545 Msa0914210:three_prime_UTR
GATACTGAGTCGGCTAATGC+TGG 0.485382 6_2:+27218708 None:intergenic
ACATCAGAAGAGTTGAGTAT+TGG 0.487462 6_2:-27218683 Msa0914210:CDS
TTATGAATTGGAAGTGTTGT+TGG 0.489479 6_2:-27217446 Msa0914210:three_prime_UTR
TAGAGTTCTTTAGTAAGAGA+TGG 0.491654 6_2:-27217505 Msa0914210:three_prime_UTR
GAGCAACTTCGCCACTGGCA+AGG 0.495672 6_2:-27217641 Msa0914210:three_prime_UTR
ATGGGTCGGACATTTGAGTC+TGG 0.503815 6_2:+27217604 None:intergenic
AGCAACTTCGCCACTGGCAA+GGG 0.507605 6_2:-27217640 Msa0914210:three_prime_UTR
CTTCGCCACTGGCAAGGGTA+AGG 0.510379 6_2:-27217635 Msa0914210:three_prime_UTR
ACAGTGGCGCAGCGAGATAA+CGG 0.512663 6_2:+27219927 None:intergenic
GTTAGACGGCGGAAACCTGC+CGG 0.513106 6_2:+27219902 None:intergenic
TGTTTCCAAGGACAAGGAAA+GGG 0.516162 6_2:+27217719 None:intergenic
AGAAGGTCAAGGATGAAATT+GGG 0.521910 6_2:-27219657 Msa0914210:CDS
GGTAGATGCAGTGGTCTACT+TGG 0.525431 6_2:-27217823 Msa0914210:CDS
GCTGCGCCACTGTTTCCGGC+AGG 0.529101 6_2:-27219917 Msa0914210:five_prime_UTR
CGAACAGAACGAGGCAACGG+CGG 0.533173 6_2:-27219625 Msa0914210:CDS
TTTGCAATGATTCATCAGAA+AGG 0.533510 6_2:+27217745 None:intergenic
TTGTTTCCAAGGACAAGGAA+AGG 0.535849 6_2:+27217718 None:intergenic
CAGCGAACAGAACGAGGCAA+CGG 0.537808 6_2:-27219628 Msa0914210:CDS
AAGAAAATGGGTTATGGTGA+TGG 0.542914 6_2:-27217556 Msa0914210:three_prime_UTR
GCTTCACATGCTCAAAGATG+AGG 0.546601 6_2:-27219590 Msa0914210:intron
GGTGTCTGAGATTGATCTAG+TGG 0.549241 6_2:+27220008 None:intergenic
TTTATTTCTATAAAATTACT+GGG 0.557099 6_2:-27217384 Msa0914210:three_prime_UTR
ATTGTGAAGAAAATGGGTTA+TGG 0.564263 6_2:-27217562 Msa0914210:three_prime_UTR
CTTGGTTGATGCTTATGACA+AGG 0.569852 6_2:-27217805 Msa0914210:CDS
ATCTTTGAGCATGTGAAGCA+AGG 0.571061 6_2:+27219594 None:intergenic
ATGGAAAGATTACTATGCCC+AGG 0.572618 6_2:-27218605 Msa0914210:intron
AGACTCAAATGTCCGACCCA+TGG 0.577020 6_2:-27217602 Msa0914210:three_prime_UTR
ACTACACATGAATACCTCCA+TGG 0.577745 6_2:+27217585 None:intergenic
ACCCAATTTCATGAGCGGAA+AGG 0.581440 6_2:+27219762 None:intergenic
ATACTCAACTCTTCTGATGT+CGG 0.590024 6_2:+27218686 None:intergenic
CTACACATGAATACCTCCAT+GGG 0.593579 6_2:+27217586 None:intergenic
TGGAAGCAGCGAACAGAACG+AGG 0.601120 6_2:-27219634 Msa0914210:CDS
CGGAAACCTGCCGGAAACAG+TGG 0.601403 6_2:+27219911 None:intergenic
GATGGTGAAGGTATGTTAGA+CGG 0.603499 6_2:+27219888 None:intergenic
TCTGATGTCGGATACTGAGT+CGG 0.603604 6_2:+27218698 None:intergenic
GAAAGCCAGAAAGATGAGGT+TGG 0.609717 6_2:-27219710 Msa0914210:exon
GAAAGAAGAATTATATGAGA+CGG 0.611804 6_2:+27219831 None:intergenic
GCCAGAAAGATGAGGTTGGT+AGG 0.616865 6_2:-27219706 Msa0914210:exon
ATTTACCTTACCCTTGCCAG+TGG 0.624440 6_2:+27217630 None:intergenic
GTAACGCAATTCGTCTTCTG+AGG 0.626984 6_2:+27217675 None:intergenic
AATTGAAAGCCAGAAAGATG+AGG 0.640929 6_2:-27219714 Msa0914210:exon
TGCAATGCAGGTAGATGCAG+TGG 0.654691 6_2:-27217832 Msa0914210:intron
TTTGAGCATGTGAAGCAAGG+TGG 0.658779 6_2:+27219597 None:intergenic
AGGTCAAGGATGAAATTGGG+TGG 0.659233 6_2:-27219654 Msa0914210:CDS
TAAACACCCAATTTCATGAG+CGG 0.660336 6_2:+27219757 None:intergenic
CTCAAATGTCCGACCCATGG+AGG 0.662910 6_2:-27217599 Msa0914210:three_prime_UTR
GTGTCTGAGATTGATCTAGT+GGG 0.665837 6_2:+27220009 None:intergenic
ACATGAATACCTCCATGGGT+CGG 0.669346 6_2:+27217590 None:intergenic
ATTGTGATTCTAAACAGCGT+GGG 0.675646 6_2:+27219856 None:intergenic
GATTGTGATTCTAAACAGCG+TGG 0.681150 6_2:+27219855 None:intergenic
GGTGAAGGTATGTTAGACGG+CGG 0.722965 6_2:+27219891 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTTTTAAATTGATTTAA+CGG + chr6_2:27219275-27219294 None:intergenic 10.0%
!!! TTTTATTTCTATAAAATTAC+TGG - chr6_2:27219976-27219995 Msa0914210:five_prime_UTR 10.0%
!!! TTTATTTCTATAAAATTACT+GGG - chr6_2:27219977-27219996 Msa0914210:five_prime_UTR 10.0%
!!! TGCTATTTTTCAAATAAAAA+CGG - chr6_2:27217559-27217578 Msa0914210:three_prime_UTR 15.0%
!!! AAAATTTTGAACAGTAAATA+AGG + chr6_2:27217899-27217918 None:intergenic 15.0%
!! ATATCTTAATCATGAAAATT+TGG - chr6_2:27218836-27218855 Msa0914210:intron 15.0%
!!! GCTATTTTTCAAATAAAAAC+GGG - chr6_2:27217560-27217579 Msa0914210:three_prime_UTR 20.0%
!! TTAACACTTATTACTATTAC+CGG - chr6_2:27217941-27217960 Msa0914210:intron 20.0%
!! TAACTTATGTAAACAACTTA+TGG - chr6_2:27218304-27218323 Msa0914210:intron 20.0%
!!! AAGTTTAAAGCTTTTGATTT+GGG - chr6_2:27218708-27218727 Msa0914210:CDS 20.0%
!!! ATGCCTTAAATACTTTTTTA+TGG - chr6_2:27218792-27218811 Msa0914210:CDS 20.0%
!!! TAGCCATAAAAAAGTATTTA+AGG + chr6_2:27218798-27218817 None:intergenic 20.0%
! GATATTTGACTCTTATGTTA+TGG + chr6_2:27217377-27217396 None:intergenic 25.0%
! GAAAGAAGAATTATATGAGA+CGG + chr6_2:27217533-27217552 None:intergenic 25.0%
!! ATCTAGAATTGAAAAACAGA+AGG - chr6_2:27217687-27217706 Msa0914210:CDS 25.0%
!! TGTTTATTTCTCAATAGTGA+CGG - chr6_2:27217865-27217884 Msa0914210:intron 25.0%
!! TTCAAAATTTTGCAGTCAAA+GGG - chr6_2:27217908-27217927 Msa0914210:intron 25.0%
!!! ATAAGTTTTGTGAGATTGTT+CGG - chr6_2:27218259-27218278 Msa0914210:intron 25.0%
! AAACAAAATGCTATATGTCA+AGG + chr6_2:27218501-27218520 None:intergenic 25.0%
! AGAAACCATAAAAGATTAAG+TGG + chr6_2:27218527-27218546 None:intergenic 25.0%
!! GTTTCTTTTATCATGAGAAT+TGG - chr6_2:27218614-27218633 Msa0914210:CDS 25.0%
!!! CAAGTTTAAAGCTTTTGATT+TGG - chr6_2:27218707-27218726 Msa0914210:CDS 25.0%
! GATTAAGATATTCTTTCACT+TGG + chr6_2:27218827-27218846 None:intergenic 25.0%
! TCCAAGACATAATTTAATCA+GGG + chr6_2:27218877-27218896 None:intergenic 25.0%
! ATTAAATTATGTCTTGGACT+TGG - chr6_2:27218879-27218898 Msa0914210:intron 25.0%
! TATAGAACAACCTAAGATAT+TGG - chr6_2:27218974-27218993 Msa0914210:intron 25.0%
! ACTATGATTGTGTTTCATTA+TGG + chr6_2:27219057-27219076 None:intergenic 25.0%
! TTATAAGCTACTGTATACAA+TGG + chr6_2:27219235-27219254 None:intergenic 25.0%
!!! GTTTTAAATTGATTTAACGG+CGG + chr6_2:27219272-27219291 None:intergenic 25.0%
! TCTCAGTTAAATATTGAACA+GGG + chr6_2:27219341-27219360 None:intergenic 25.0%
! TTCTCAGTTAAATATTGAAC+AGG + chr6_2:27219342-27219361 None:intergenic 25.0%
! CTAAAAGCAATATAGTGAAA+AGG + chr6_2:27219392-27219411 None:intergenic 25.0%
!! TGCTTTTAGTACCTTATAAA+TGG - chr6_2:27219403-27219422 Msa0914210:intron 25.0%
!!! GAATATCAATTTTGTTTCCA+AGG + chr6_2:27219657-27219676 None:intergenic 25.0%
! TGTAGTATTGTGAAGAAAAT+GGG - chr6_2:27219793-27219812 Msa0914210:five_prime_UTR 25.0%
! CCAAAAATTAACTGAATTGT+AGG + chr6_2:27220005-27220024 None:intergenic 25.0%
!! CCTACAATTCAGTTAATTTT+TGG - chr6_2:27220002-27220021 Msa0914210:five_prime_UTR 25.0%
AATTGAAAAACAGAAGGTCA+AGG - chr6_2:27217693-27217712 Msa0914210:CDS 30.0%
! GTTCAAAATTTTGCAGTCAA+AGG - chr6_2:27217907-27217926 Msa0914210:intron 30.0%
! TCAAAATTTTGCAGTCAAAG+GGG - chr6_2:27217909-27217928 Msa0914210:intron 30.0%
!! TCGTATTGATTAGAAAACTC+CGG + chr6_2:27217963-27217982 None:intergenic 30.0%
TCACATGTTTATGTCAACAA+AGG + chr6_2:27218133-27218152 None:intergenic 30.0%
! GCCATGTTTTTCTATGTTTA+GGG - chr6_2:27218199-27218218 Msa0914210:intron 30.0%
TTATATGCAACTTAAAGCAC+AGG + chr6_2:27218422-27218441 None:intergenic 30.0%
AACACAGCAAAACAAGTTAA+GGG + chr6_2:27218463-27218482 None:intergenic 30.0%
! TTCGACCACTTAATCTTTTA+TGG - chr6_2:27218519-27218538 Msa0914210:intron 30.0%
ATATCTTTATCTCTGAGTCT+TGG - chr6_2:27218585-27218604 Msa0914210:intron 30.0%
!!! TTTAAAGCTTTTGATTTGGG+TGG - chr6_2:27218711-27218730 Msa0914210:CDS 30.0%
!!! TTAAAGCTTTTGATTTGGGT+GGG - chr6_2:27218712-27218731 Msa0914210:CDS 30.0%
AGACATAATTTAATCAGGGA+AGG + chr6_2:27218873-27218892 None:intergenic 30.0%
TCCCTGATTAAATTATGTCT+TGG - chr6_2:27218873-27218892 Msa0914210:intron 30.0%
GTCCAAGACATAATTTAATC+AGG + chr6_2:27218878-27218897 None:intergenic 30.0%
AGATTCTAAGCCAATATCTT+AGG + chr6_2:27218987-27219006 None:intergenic 30.0%
ATACAATGGATAGAATGCAA+GGG + chr6_2:27219221-27219240 None:intergenic 30.0%
TATACAATGGATAGAATGCA+AGG + chr6_2:27219222-27219241 None:intergenic 30.0%
!!! AACGTTTTAACTGAGAAACA+AGG - chr6_2:27219306-27219325 Msa0914210:intron 30.0%
TTTAACTGAGAAACAAGGTT+TGG - chr6_2:27219311-27219330 Msa0914210:intron 30.0%
TTGATTCAGAGCCATTTATA+AGG + chr6_2:27219417-27219436 None:intergenic 30.0%
!! AAGATTTGCTGAGTCAAAAA+AGG - chr6_2:27219580-27219599 Msa0914210:intron 30.0%
TTTGCAATGATTCATCAGAA+AGG + chr6_2:27219619-27219638 None:intergenic 30.0%
GTGTAGTATTGTGAAGAAAA+TGG - chr6_2:27219792-27219811 Msa0914210:five_prime_UTR 30.0%
!! ATTGTGAAGAAAATGGGTTA+TGG - chr6_2:27219799-27219818 Msa0914210:five_prime_UTR 30.0%
!! TTATGGTGATGGTTTCAAAT+GGG - chr6_2:27219816-27219835 Msa0914210:five_prime_UTR 30.0%
TAGAGTTCTTTAGTAAGAGA+TGG - chr6_2:27219856-27219875 Msa0914210:five_prime_UTR 30.0%
TAGTCTCTGTACTTATGAAT+TGG - chr6_2:27219903-27219922 Msa0914210:five_prime_UTR 30.0%
!! TTATGAATTGGAAGTGTTGT+TGG - chr6_2:27219915-27219934 Msa0914210:five_prime_UTR 30.0%
!! TTAGCTTTGGGCTATGAAAT+GGG + chr6_2:27217405-27217424 None:intergenic 35.0%
!! TTTAGCTTTGGGCTATGAAA+TGG + chr6_2:27217406-27217425 None:intergenic 35.0%
ATTGTGATTCTAAACAGCGT+GGG + chr6_2:27217508-27217527 None:intergenic 35.0%
TAAACACCCAATTTCATGAG+CGG + chr6_2:27217607-27217626 None:intergenic 35.0%
AATTGAAAGCCAGAAAGATG+AGG - chr6_2:27217647-27217666 Msa0914210:exon 35.0%
AGAAGGTCAAGGATGAAATT+GGG - chr6_2:27217704-27217723 Msa0914210:CDS 35.0%
TCATTCCAAAAGAACAACGT+CGG + chr6_2:27217839-27217858 None:intergenic 35.0%
CATGTTTATGTCAACAAAGG+TGG + chr6_2:27218130-27218149 None:intergenic 35.0%
! GGCCATGTTTTTCTATGTTT+AGG - chr6_2:27218198-27218217 Msa0914210:intron 35.0%
GCCCTAAACATAGAAAAACA+TGG + chr6_2:27218203-27218222 None:intergenic 35.0%
TTAAATAAGCCAATCCAACC+AGG + chr6_2:27218225-27218244 None:intergenic 35.0%
! AAACAAGTTAAGGGCATGTT+TGG + chr6_2:27218454-27218473 None:intergenic 35.0%
CAACACAGCAAAACAAGTTA+AGG + chr6_2:27218464-27218483 None:intergenic 35.0%
ATACTCAACTCTTCTGATGT+CGG + chr6_2:27218678-27218697 None:intergenic 35.0%
ACATCAGAAGAGTTGAGTAT+TGG - chr6_2:27218678-27218697 Msa0914210:CDS 35.0%
CATCAGAAGAGTTGAGTATT+GGG - chr6_2:27218679-27218698 Msa0914210:CDS 35.0%
ATGAAAATTTGGATGCCACT+AGG - chr6_2:27218847-27218866 Msa0914210:intron 35.0%
!! CAATTTTGTTTCCAAGGACA+AGG + chr6_2:27219651-27219670 None:intergenic 35.0%
!! AAGAAAATGGGTTATGGTGA+TGG - chr6_2:27219805-27219824 Msa0914210:five_prime_UTR 35.0%
!! GTTATGGTGATGGTTTCAAA+TGG - chr6_2:27219815-27219834 Msa0914210:five_prime_UTR 35.0%
TTCTTTAGTAAGAGATGGCT+TGG - chr6_2:27219861-27219880 Msa0914210:five_prime_UTR 35.0%
! GATGGTGAAGGTATGTTAGA+CGG + chr6_2:27217476-27217495 None:intergenic 40.0%
GATTGTGATTCTAAACAGCG+TGG + chr6_2:27217509-27217528 None:intergenic 40.0%
CAGAAGGTCAAGGATGAAAT+TGG - chr6_2:27217703-27217722 Msa0914210:CDS 40.0%
ATCTTTGAGCATGTGAAGCA+AGG + chr6_2:27217770-27217789 None:intergenic 40.0%
! TGTTACCGACGTTGTTCTTT+TGG - chr6_2:27217831-27217850 Msa0914210:intron 40.0%
AAAACATGGCCAATCATGCA+TGG + chr6_2:27218189-27218208 None:intergenic 40.0%
!! GTTTTTCTATGTTTAGGGCC+TGG - chr6_2:27218204-27218223 Msa0914210:intron 40.0%
!! TTGTTTTGCTGTGTTGCTCT+TGG - chr6_2:27218468-27218487 Msa0914210:intron 40.0%
ATCAGATTGCTCGTAGAGTA+TGG - chr6_2:27218737-27218756 Msa0914210:CDS 40.0%
ATGGAAAGATTACTATGCCC+AGG - chr6_2:27218756-27218775 Msa0914210:CDS 40.0%
GCATAGAAAAAGAGAGACCT+GGG + chr6_2:27218776-27218795 None:intergenic 40.0%
AATCGAGAAACATTACGTGC+TGG - chr6_2:27219466-27219485 Msa0914210:intron 40.0%
!! TCATGAGTTTGTTGCAATGC+AGG - chr6_2:27219517-27219536 Msa0914210:intron 40.0%
! CTTGGTTGATGCTTATGACA+AGG - chr6_2:27219556-27219575 Msa0914210:intron 40.0%
TGTTTCCAAGGACAAGGAAA+GGG + chr6_2:27219645-27219664 None:intergenic 40.0%
TTGTTTCCAAGGACAAGGAA+AGG + chr6_2:27219646-27219665 None:intergenic 40.0%
AGACGAATTGCGTTACCATT+TGG - chr6_2:27219693-27219712 Msa0914210:CDS 40.0%
GACGAATTGCGTTACCATTT+GGG - chr6_2:27219694-27219713 Msa0914210:CDS 40.0%
CTACACATGAATACCTCCAT+GGG + chr6_2:27219778-27219797 None:intergenic 40.0%
ACTACACATGAATACCTCCA+TGG + chr6_2:27219779-27219798 None:intergenic 40.0%
!! CGGCAACGAAGTTTAGCTTT+GGG + chr6_2:27217417-27217436 None:intergenic 45.0%
! ACGGCAACGAAGTTTAGCTT+TGG + chr6_2:27217418-27217437 None:intergenic 45.0%
!!! GGGTGTTTTTGAGATGGTGA+AGG + chr6_2:27217488-27217507 None:intergenic 45.0%
TCCCTTTCCGCTCATGAAAT+TGG - chr6_2:27217597-27217616 Msa0914210:three_prime_UTR 45.0%
CCCAATTTCATGAGCGGAAA+GGG + chr6_2:27217601-27217620 None:intergenic 45.0%
CCCTTTCCGCTCATGAAATT+GGG - chr6_2:27217598-27217617 Msa0914210:three_prime_UTR 45.0%
ACCCAATTTCATGAGCGGAA+AGG + chr6_2:27217602-27217621 None:intergenic 45.0%
GAAAGCCAGAAAGATGAGGT+TGG - chr6_2:27217651-27217670 Msa0914210:exon 45.0%
TCCTACCAACCTCATCTTTC+TGG + chr6_2:27217659-27217678 None:intergenic 45.0%
AGGTCAAGGATGAAATTGGG+TGG - chr6_2:27217707-27217726 Msa0914210:CDS 45.0%
TTTGAGCATGTGAAGCAAGG+TGG + chr6_2:27217767-27217786 None:intergenic 45.0%
GCTTCACATGCTCAAAGATG+AGG - chr6_2:27217771-27217790 Msa0914210:CDS 45.0%
TCATGCATGGAGGAAGGTAA+TGG + chr6_2:27218176-27218195 None:intergenic 45.0%
TACCTTCCTCCATGCATGAT+TGG - chr6_2:27218177-27218196 Msa0914210:intron 45.0%
! TTCTATGTTTAGGGCCTGGT+TGG - chr6_2:27218208-27218227 Msa0914210:intron 45.0%
TCTGATGTCGGATACTGAGT+CGG + chr6_2:27218666-27218685 None:intergenic 45.0%
GGCATAGAAAAAGAGAGACC+TGG + chr6_2:27218777-27218796 None:intergenic 45.0%
TTTAATCAGGGAAGGCCTAG+TGG + chr6_2:27218865-27218884 None:intergenic 45.0%
AATGTCCCTTTCCTTGTCCT+TGG - chr6_2:27219637-27219656 Msa0914210:CDS 45.0%
!! TCGTCTTCTGAGGCAACATA+TGG + chr6_2:27219679-27219698 None:intergenic 45.0%
GTAACGCAATTCGTCTTCTG+AGG + chr6_2:27219689-27219708 None:intergenic 45.0%
ATTTACCTTACCCTTGCCAG+TGG + chr6_2:27219734-27219753 None:intergenic 45.0%
ACATGAATACCTCCATGGGT+CGG + chr6_2:27219774-27219793 None:intergenic 45.0%
! GGTGAAGGTATGTTAGACGG+CGG + chr6_2:27217473-27217492 None:intergenic 50.0%
!! CAGCGTGGGTGTTTTTGAGA+TGG + chr6_2:27217494-27217513 None:intergenic 50.0%
! GCCAGAAAGATGAGGTTGGT+AGG - chr6_2:27217655-27217674 Msa0914210:exon 50.0%
ACATGGCCAATCATGCATGG+AGG + chr6_2:27218186-27218205 None:intergenic 50.0%
! TGTTTAGGGCCTGGTTGGAT+TGG - chr6_2:27218213-27218232 Msa0914210:intron 50.0%
GATACTGAGTCGGCTAATGC+TGG + chr6_2:27218656-27218675 None:intergenic 50.0%
TGCAATGCAGGTAGATGCAG+TGG - chr6_2:27219529-27219548 Msa0914210:intron 50.0%
GGTAGATGCAGTGGTCTACT+TGG - chr6_2:27219538-27219557 Msa0914210:intron 50.0%
CGAAGTTGCTCAAGCCCAAA+TGG + chr6_2:27219711-27219730 None:intergenic 50.0%
ATGGGTCGGACATTTGAGTC+TGG + chr6_2:27219760-27219779 None:intergenic 50.0%
AGACTCAAATGTCCGACCCA+TGG - chr6_2:27219759-27219778 Msa0914210:five_prime_UTR 50.0%
ACAGTGGCGCAGCGAGATAA+CGG + chr6_2:27217437-27217456 None:intergenic 55.0%
TGGAAGCAGCGAACAGAACG+AGG - chr6_2:27217727-27217746 Msa0914210:CDS 55.0%
CAGCGAACAGAACGAGGCAA+CGG - chr6_2:27217733-27217752 Msa0914210:CDS 55.0%
GGCCAATCATGCATGGAGGA+AGG + chr6_2:27218182-27218201 None:intergenic 55.0%
AGCAACTTCGCCACTGGCAA+GGG - chr6_2:27219721-27219740 Msa0914210:five_prime_UTR 55.0%
CTCAAATGTCCGACCCATGG+AGG - chr6_2:27219762-27219781 Msa0914210:five_prime_UTR 55.0%
TCTCGCTGCGCCACTGTTTC+CGG - chr6_2:27217440-27217459 Msa0914210:three_prime_UTR 60.0%
CGGAAACCTGCCGGAAACAG+TGG + chr6_2:27217453-27217472 None:intergenic 60.0%
GTTAGACGGCGGAAACCTGC+CGG + chr6_2:27217462-27217481 None:intergenic 60.0%
CGAACAGAACGAGGCAACGG+CGG - chr6_2:27217736-27217755 Msa0914210:CDS 60.0%
GGCTTGAGCAACTTCGCCAC+TGG - chr6_2:27219715-27219734 Msa0914210:exon 60.0%
GAGCAACTTCGCCACTGGCA+AGG - chr6_2:27219720-27219739 Msa0914210:five_prime_UTR 60.0%
CTTCGCCACTGGCAAGGGTA+AGG - chr6_2:27219726-27219745 Msa0914210:five_prime_UTR 60.0%
GCTGCGCCACTGTTTCCGGC+AGG - chr6_2:27217444-27217463 Msa0914210:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 27217355 27220028 27217355 ID=Msa0914210;Name=Msa0914210
chr6_2 mRNA 27217355 27220028 27217355 ID=Msa0914210-mRNA-1;Parent=Msa0914210;Name=Msa0914210-mRNA-1;_AED=0.29;_eAED=0.29;_QI=309|0.33|0.5|1|0|0.5|4|307|169
chr6_2 exon 27217355 27217844 27217355 ID=Msa0914210-mRNA-1:exon:5120;Parent=Msa0914210-mRNA-1
chr6_2 exon 27218606 27218665 27218606 ID=Msa0914210-mRNA-1:exon:5119;Parent=Msa0914210-mRNA-1
chr6_2 exon 27218674 27218811 27218674 ID=Msa0914210-mRNA-1:exon:5118;Parent=Msa0914210-mRNA-1
chr6_2 exon 27219591 27220028 27219591 ID=Msa0914210-mRNA-1:exon:5117;Parent=Msa0914210-mRNA-1
chr6_2 five_prime_UTR 27219720 27220028 27219720 ID=Msa0914210-mRNA-1:five_prime_utr;Parent=Msa0914210-mRNA-1
chr6_2 CDS 27219591 27219719 27219591 ID=Msa0914210-mRNA-1:cds;Parent=Msa0914210-mRNA-1
chr6_2 CDS 27218674 27218811 27218674 ID=Msa0914210-mRNA-1:cds;Parent=Msa0914210-mRNA-1
chr6_2 CDS 27218606 27218665 27218606 ID=Msa0914210-mRNA-1:cds;Parent=Msa0914210-mRNA-1
chr6_2 CDS 27217662 27217844 27217662 ID=Msa0914210-mRNA-1:cds;Parent=Msa0914210-mRNA-1
chr6_2 three_prime_UTR 27217355 27217661 27217355 ID=Msa0914210-mRNA-1:three_prime_utr;Parent=Msa0914210-mRNA-1
Gene Sequence

>Msa0914210

ATGAGGTTGGTAGGAAGCAACGAATCTAGAATTGAAAAACAGAAGGTCAAGGATGAAATTGGGTGGAAGCAGCGAACAGAACGAGGCAACGGCGGCGAAACCACCTTGCTTCACATGCTCAAAGATGAGCAAGTGTGTGTTTATATCTTTATCTCTGAGTCTTGGTGTAATGTTTCTTTTATCATGAGAATTGGTTTTGCAGCGATTAGTCCAGCATTAGCCGACTCAGTATCCGACATCAGAAGAGTTGAGTATTGGGAAGATCAACTTTTGATTTGGGTGGGTCATCAGATTGCTCGTAGAGTATGGAAAGATTACTATGCCCAGGTAGATGCAGTGGTCTACTTGGTTGATGCTTATGACAAGGAAAGATTTGCTGAGTCAAAAAAGGAGTTAGATGCTCTCCTTTCTGATGAATCATTGCAAATGTCCCTTTCCTTGTCCTTGGAAACAAAATTGATATTCCATATGTTGCCTCAGAAGACGAATTGCGTTACCATTTGGGCTTGA
Protein sequence

>Msa0914210

MRLVGSNESRIEKQKVKDEIGWKQRTERGNGGETTLLHMLKDEQVCVYIFISESWCNVSFIMRIGFAAISPALADSVSDIRRVEYWEDQLLIWVGHQIARRVWKDYYAQVDAVVYLVDAYDKERFAESKKELDALLSDESLQMSLSLSLETKLIFHMLPQKTNCVTIWA*