AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0914650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0914650 MtrunA17_Chr6g0469681 61.475 122 21 2 1 107 1 111 1.16e-32 111
Msa0914650 MtrunA17_Chr6g0469651 59.829 117 27 2 1 102 1 112 1.04e-29 103
Msa0914650 MtrunA17_Chr6g0469671 59.483 116 23 2 1 101 1 107 3.81e-25 91.7
Msa0914650 MtrunA17_Chr7g0233201 51.220 82 25 1 1 67 1 82 9.28e-21 80.5
Msa0914650 MtrunA17_Chr7g0233191 51.220 82 25 1 1 67 1 82 1.12e-20 80.5
Msa0914650 MtrunA17_Chr6g0469571 50.442 113 26 3 1 98 1 98 1.29e-20 80.5
Msa0914650 MtrunA17_Chr7g0233251 52.439 82 24 1 1 67 1 82 1.33e-20 80.1
Msa0914650 MtrunA17_Chr7g0233181 50.000 82 26 1 1 67 1 82 4.26e-20 78.6
Msa0914650 MtrunA17_Chr6g0469551 49.558 113 27 3 1 98 1 98 4.65e-20 79.3
Msa0914650 MtrunA17_Chr6g0469591 50.442 113 26 3 1 98 1 98 4.96e-20 79.0
Msa0914650 MtrunA17_Chr6g0469541 39.516 124 35 3 1 105 1 103 5.16e-20 78.6
Msa0914650 MtrunA17_Chr1g0202581 56.522 69 16 1 1 55 1 69 5.69e-20 79.3
Msa0914650 MtrunA17_Chr7g0233231 50.000 82 26 1 1 67 1 82 7.14e-20 78.2
Msa0914650 MtrunA17_Chr1g0204291 53.623 69 18 1 1 55 1 69 3.54e-19 77.0
Msa0914650 MtrunA17_Chr7g0233211 48.780 82 27 1 1 67 1 82 4.28e-19 76.3
Msa0914650 MtrunA17_Chr8g0387471 36.842 133 54 3 1 118 1 118 6.31e-19 76.3
Msa0914650 MtrunA17_Chr1g0202541 54.286 70 17 1 1 55 1 70 1.67e-18 75.5
Msa0914650 MtrunA17_Chr1g0202561 55.556 72 17 1 1 57 1 72 1.41e-17 73.2
Msa0914650 MtrunA17_Chr1g0202501 47.059 68 21 1 1 53 1 68 1.14e-13 62.8
Msa0914650 MtrunA17_Chr7g0248671 47.059 68 21 1 1 53 1 68 2.04e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0914650 AT1G01490.1 50.704 71 19 2 1 55 1 71 5.47e-13 62.4
Msa0914650 AT1G01490.2 50.704 71 19 2 1 55 1 71 5.47e-13 62.4
Msa0914650 AT1G01490.4 49.275 69 19 2 3 55 30 98 1.56e-11 58.9

Find 18 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATATTGAAATATTATTTA+TGG 0.092295 6_2:+29358768 Msa0914650:CDS
TCAATATATCTTAACAAATA+AGG 0.215104 6_2:-29358754 None:intergenic
GAAGGAGGAGCCAAAGAAAA+AGG 0.237298 6_2:+29358660 Msa0914650:CDS
GATAAGAAAATGACAATAAT+TGG 0.247507 6_2:+29358505 Msa0914650:CDS
ATGACAGAAACTAGTGTTAA+AGG 0.285053 6_2:+29358010 Msa0914650:intron
AACTTCGACACAACATTAAC+AGG 0.307594 6_2:-29358538 None:intergenic
TATCTTAACAAATAAGGATA+TGG 0.344061 6_2:-29358748 None:intergenic
AAAGGACACAAAAGATGAAT+TGG 0.372542 6_2:+29358678 Msa0914650:CDS
GAGACCAGATACCGTCTTCA+TGG 0.381713 6_2:-29358066 None:intergenic
TTAATGTTGTGTCGAAGTTA+AGG 0.400464 6_2:+29358542 Msa0914650:CDS
CATGCTGAAATAATTGCAAT+TGG 0.461465 6_2:+29358574 Msa0914650:CDS
TACAGTTGCTAAGGAAGGTC+AGG 0.464456 6_2:-29357686 None:intergenic
TCATATCGATTTGAAGCTAG+TGG 0.498363 6_2:-29357711 None:intergenic
TGAATTGGCTGATCTTATGA+AGG 0.506423 6_2:+29358693 Msa0914650:CDS
CTAGCTTCAAATCGATATGA+AGG 0.515190 6_2:+29357714 Msa0914650:exon
CTAGTGGGTTACAGTTGCTA+AGG 0.551629 6_2:-29357695 None:intergenic
CATATCGATTTGAAGCTAGT+GGG 0.555444 6_2:-29357710 None:intergenic
TGGGTTACAGTTGCTAAGGA+AGG 0.623584 6_2:-29357691 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATAAATTAAATCAAATA+TGG - chr6_2:29357838-29357857 None:intergenic 10.0%
!!! TAAAATATTTCTTAAGTTTT+TGG - chr6_2:29358332-29358351 None:intergenic 10.0%
!!! AAAAACTTAAGAAATATTTT+AGG + chr6_2:29358331-29358350 Msa0914650:intron 10.0%
!!! AAAACTTAAGAAATATTTTA+GGG + chr6_2:29358332-29358351 Msa0914650:intron 10.0%
!!! ATTTTTTATTTTTCTTTTTC+AGG + chr6_2:29358454-29358473 Msa0914650:intron 10.0%
!!! TTTTTTATTTTTCTTTTTCA+GGG + chr6_2:29358455-29358474 Msa0914650:intron 10.0%
!! TTGATAAATTAAATCAAATA+TGG - chr6_2:29357838-29357857 None:intergenic 10.0%
!!! TAAAATATTTCTTAAGTTTT+TGG - chr6_2:29358332-29358351 None:intergenic 10.0%
!!! AAAAACTTAAGAAATATTTT+AGG + chr6_2:29358331-29358350 Msa0914650:intron 10.0%
!!! AAAACTTAAGAAATATTTTA+GGG + chr6_2:29358332-29358351 Msa0914650:intron 10.0%
!!! ATTTTTTATTTTTCTTTTTC+AGG + chr6_2:29358454-29358473 Msa0914650:intron 10.0%
!!! TTTTTTATTTTTCTTTTTCA+GGG + chr6_2:29358455-29358474 Msa0914650:intron 10.0%
!! AATTAAATCAAATATGGAAA+GGG - chr6_2:29357832-29357851 None:intergenic 15.0%
!! AAATTAAATCAAATATGGAA+AGG - chr6_2:29357833-29357852 None:intergenic 15.0%
!! TGTAATTTAATAACTTAAAC+TGG - chr6_2:29357923-29357942 None:intergenic 15.0%
!!! ATTTCATGTATGAAAATTTT+TGG + chr6_2:29358301-29358320 Msa0914650:intron 15.0%
!!! AAACTTAAGAAATATTTTAG+GGG + chr6_2:29358333-29358352 Msa0914650:intron 15.0%
!!! TTTTTATTTTTCTTTTTCAG+GGG + chr6_2:29358456-29358475 Msa0914650:intron 15.0%
!! TCAATATATCTTAACAAATA+AGG - chr6_2:29358757-29358776 None:intergenic 15.0%
!! AATTAAATCAAATATGGAAA+GGG - chr6_2:29357832-29357851 None:intergenic 15.0%
!! AAATTAAATCAAATATGGAA+AGG - chr6_2:29357833-29357852 None:intergenic 15.0%
!! TGTAATTTAATAACTTAAAC+TGG - chr6_2:29357923-29357942 None:intergenic 15.0%
!!! ATTTCATGTATGAAAATTTT+TGG + chr6_2:29358301-29358320 Msa0914650:intron 15.0%
!!! AAACTTAAGAAATATTTTAG+GGG + chr6_2:29358333-29358352 Msa0914650:intron 15.0%
!!! TTTTTATTTTTCTTTTTCAG+GGG + chr6_2:29358456-29358475 Msa0914650:intron 15.0%
!! TCAATATATCTTAACAAATA+AGG - chr6_2:29358757-29358776 None:intergenic 15.0%
!! TAATAATTATGACAGAAAAC+TGG - chr6_2:29357765-29357784 None:intergenic 20.0%
!!! AACTTAAGAAATATTTTAGG+GGG + chr6_2:29358334-29358353 Msa0914650:intron 20.0%
!!! TATTCAACCTTTAAAAAGTT+AGG + chr6_2:29358397-29358416 Msa0914650:intron 20.0%
!!! ATTCAACCTTTAAAAAGTTA+GGG + chr6_2:29358398-29358417 Msa0914650:intron 20.0%
!! GATAAGAAAATGACAATAAT+TGG + chr6_2:29358505-29358524 Msa0914650:CDS 20.0%
!! AAAGAAAGAAGAAAAAAAGA+AGG + chr6_2:29358642-29358661 Msa0914650:CDS 20.0%
!! TATCTTAACAAATAAGGATA+TGG - chr6_2:29358751-29358770 None:intergenic 20.0%
!! TAATAATTATGACAGAAAAC+TGG - chr6_2:29357765-29357784 None:intergenic 20.0%
!!! AACTTAAGAAATATTTTAGG+GGG + chr6_2:29358334-29358353 Msa0914650:intron 20.0%
!!! TATTCAACCTTTAAAAAGTT+AGG + chr6_2:29358397-29358416 Msa0914650:intron 20.0%
!!! ATTCAACCTTTAAAAAGTTA+GGG + chr6_2:29358398-29358417 Msa0914650:intron 20.0%
!! GATAAGAAAATGACAATAAT+TGG + chr6_2:29358505-29358524 Msa0914650:CDS 20.0%
!! AAAGAAAGAAGAAAAAAAGA+AGG + chr6_2:29358642-29358661 Msa0914650:CDS 20.0%
!! TATCTTAACAAATAAGGATA+TGG - chr6_2:29358751-29358770 None:intergenic 20.0%
! AAAGGGAAAAAATTGAGAAA+TGG - chr6_2:29357815-29357834 None:intergenic 25.0%
! TCTCAAATTGATTATTCCAA+AGG - chr6_2:29357966-29357985 None:intergenic 25.0%
! TGCATAAAATGATTAGATGT+TGG - chr6_2:29358106-29358125 None:intergenic 25.0%
! AAAGGGAAAAAATTGAGAAA+TGG - chr6_2:29357815-29357834 None:intergenic 25.0%
! TCTCAAATTGATTATTCCAA+AGG - chr6_2:29357966-29357985 None:intergenic 25.0%
! TGCATAAAATGATTAGATGT+TGG - chr6_2:29358106-29358125 None:intergenic 25.0%
AGAAAACTGGAAGGAAAAAA+AGG - chr6_2:29357752-29357771 None:intergenic 30.0%
AATTATGACAGAAAACTGGA+AGG - chr6_2:29357761-29357780 None:intergenic 30.0%
AAAGGCAAACTAAAAGAGAA+CGG - chr6_2:29357948-29357967 None:intergenic 30.0%
ATGACAGAAACTAGTGTTAA+AGG + chr6_2:29358010-29358029 Msa0914650:intron 30.0%
! ATCATTTTATGCAACATCTC+AGG + chr6_2:29358113-29358132 Msa0914650:intron 30.0%
! GAATTTTATACCTGAGATGT+TGG - chr6_2:29358148-29358167 None:intergenic 30.0%
! TTTCCTCCCTAACTTTTTAA+AGG - chr6_2:29358407-29358426 None:intergenic 30.0%
TAAAAAGTTAGGGAGGAAAA+AGG + chr6_2:29358408-29358427 Msa0914650:intron 30.0%
TTAATGTTGTGTCGAAGTTA+AGG + chr6_2:29358542-29358561 Msa0914650:CDS 30.0%
CATGCTGAAATAATTGCAAT+TGG + chr6_2:29358574-29358593 Msa0914650:CDS 30.0%
!!! TCTTTTTTCTCTTCTTTTGC+TGG - chr6_2:29358601-29358620 None:intergenic 30.0%
AGCAAAAGAAGAGAAAAAAG+AGG + chr6_2:29358600-29358619 Msa0914650:CDS 30.0%
GAAAGAAGAAAAAAAGAAGG+AGG + chr6_2:29358645-29358664 Msa0914650:CDS 30.0%
! TCTTTTGTGTCCTTTTTCTT+TGG - chr6_2:29358673-29358692 None:intergenic 30.0%
AAAGGACACAAAAGATGAAT+TGG + chr6_2:29358678-29358697 Msa0914650:CDS 30.0%
AGAAAACTGGAAGGAAAAAA+AGG - chr6_2:29357752-29357771 None:intergenic 30.0%
AATTATGACAGAAAACTGGA+AGG - chr6_2:29357761-29357780 None:intergenic 30.0%
AAAGGCAAACTAAAAGAGAA+CGG - chr6_2:29357948-29357967 None:intergenic 30.0%
ATGACAGAAACTAGTGTTAA+AGG + chr6_2:29358010-29358029 Msa0914650:intron 30.0%
! ATCATTTTATGCAACATCTC+AGG + chr6_2:29358113-29358132 Msa0914650:intron 30.0%
! GAATTTTATACCTGAGATGT+TGG - chr6_2:29358148-29358167 None:intergenic 30.0%
! TTTCCTCCCTAACTTTTTAA+AGG - chr6_2:29358407-29358426 None:intergenic 30.0%
TAAAAAGTTAGGGAGGAAAA+AGG + chr6_2:29358408-29358427 Msa0914650:intron 30.0%
TTAATGTTGTGTCGAAGTTA+AGG + chr6_2:29358542-29358561 Msa0914650:CDS 30.0%
CATGCTGAAATAATTGCAAT+TGG + chr6_2:29358574-29358593 Msa0914650:CDS 30.0%
!!! TCTTTTTTCTCTTCTTTTGC+TGG - chr6_2:29358601-29358620 None:intergenic 30.0%
AGCAAAAGAAGAGAAAAAAG+AGG + chr6_2:29358600-29358619 Msa0914650:CDS 30.0%
GAAAGAAGAAAAAAAGAAGG+AGG + chr6_2:29358645-29358664 Msa0914650:CDS 30.0%
! TCTTTTGTGTCCTTTTTCTT+TGG - chr6_2:29358673-29358692 None:intergenic 30.0%
AAAGGACACAAAAGATGAAT+TGG + chr6_2:29358678-29358697 Msa0914650:CDS 30.0%
!! CATATCGATTTGAAGCTAGT+GGG - chr6_2:29357713-29357732 None:intergenic 35.0%
!! TCATATCGATTTGAAGCTAG+TGG - chr6_2:29357714-29357733 None:intergenic 35.0%
CTAGCTTCAAATCGATATGA+AGG + chr6_2:29357714-29357733 Msa0914650:exon 35.0%
CTTAAACTGGATCTCAAACT+CGG - chr6_2:29357910-29357929 None:intergenic 35.0%
AAGGCAAACTAAAAGAGAAC+GGG - chr6_2:29357947-29357966 None:intergenic 35.0%
!!! GTTCTCTTTTAGTTTGCCTT+TGG + chr6_2:29357947-29357966 Msa0914650:intron 35.0%
CAAGAAAAAAGCCATGAAGA+CGG + chr6_2:29358055-29358074 Msa0914650:CDS 35.0%
GTATGAAATTCCAACATCTC+AGG + chr6_2:29358135-29358154 Msa0914650:intron 35.0%
! CAACCTTTAAAAAGTTAGGG+AGG + chr6_2:29358401-29358420 Msa0914650:intron 35.0%
!!! TTTTTCTTTTTCAGGGGTTG+AGG + chr6_2:29358462-29358481 Msa0914650:intron 35.0%
AACTTCGACACAACATTAAC+AGG - chr6_2:29358541-29358560 None:intergenic 35.0%
TGAATTGGCTGATCTTATGA+AGG + chr6_2:29358693-29358712 Msa0914650:CDS 35.0%
!! CATATCGATTTGAAGCTAGT+GGG - chr6_2:29357713-29357732 None:intergenic 35.0%
!! TCATATCGATTTGAAGCTAG+TGG - chr6_2:29357714-29357733 None:intergenic 35.0%
CTAGCTTCAAATCGATATGA+AGG + chr6_2:29357714-29357733 Msa0914650:exon 35.0%
CTTAAACTGGATCTCAAACT+CGG - chr6_2:29357910-29357929 None:intergenic 35.0%
AAGGCAAACTAAAAGAGAAC+GGG - chr6_2:29357947-29357966 None:intergenic 35.0%
!!! GTTCTCTTTTAGTTTGCCTT+TGG + chr6_2:29357947-29357966 Msa0914650:intron 35.0%
CAAGAAAAAAGCCATGAAGA+CGG + chr6_2:29358055-29358074 Msa0914650:CDS 35.0%
GTATGAAATTCCAACATCTC+AGG + chr6_2:29358135-29358154 Msa0914650:intron 35.0%
! CAACCTTTAAAAAGTTAGGG+AGG + chr6_2:29358401-29358420 Msa0914650:intron 35.0%
!!! TTTTTCTTTTTCAGGGGTTG+AGG + chr6_2:29358462-29358481 Msa0914650:intron 35.0%
AACTTCGACACAACATTAAC+AGG - chr6_2:29358541-29358560 None:intergenic 35.0%
TGAATTGGCTGATCTTATGA+AGG + chr6_2:29358693-29358712 Msa0914650:CDS 35.0%
! GGTTGTTTCATTGGACATGA+AGG + chr6_2:29358483-29358502 Msa0914650:intron 40.0%
! GGTTGTTTCATTGGACATGA+AGG + chr6_2:29358483-29358502 Msa0914650:intron 40.0%
TACAGTTGCTAAGGAAGGTC+AGG - chr6_2:29357689-29357708 None:intergenic 45.0%
TGGGTTACAGTTGCTAAGGA+AGG - chr6_2:29357694-29357713 None:intergenic 45.0%
!! CTAGTGGGTTACAGTTGCTA+AGG - chr6_2:29357698-29357717 None:intergenic 45.0%
AAAGCCATGAAGACGGTATC+TGG + chr6_2:29358062-29358081 Msa0914650:intron 45.0%
! AGGGGTTGAGGTTGTTTCAT+TGG + chr6_2:29358474-29358493 Msa0914650:intron 45.0%
GAAGGAGGAGCCAAAGAAAA+AGG + chr6_2:29358660-29358679 Msa0914650:CDS 45.0%
TACAGTTGCTAAGGAAGGTC+AGG - chr6_2:29357689-29357708 None:intergenic 45.0%
TGGGTTACAGTTGCTAAGGA+AGG - chr6_2:29357694-29357713 None:intergenic 45.0%
!! CTAGTGGGTTACAGTTGCTA+AGG - chr6_2:29357698-29357717 None:intergenic 45.0%
AAAGCCATGAAGACGGTATC+TGG + chr6_2:29358062-29358081 Msa0914650:intron 45.0%
! AGGGGTTGAGGTTGTTTCAT+TGG + chr6_2:29358474-29358493 Msa0914650:intron 45.0%
GAAGGAGGAGCCAAAGAAAA+AGG + chr6_2:29358660-29358679 Msa0914650:CDS 45.0%
!! AAATTTATTAAAAAATACAA+AGG + chr6_2:29358245-29358264 Msa0914650:intron 5.0%
!! ATATATTGAAATATTATTTA+TGG + chr6_2:29358768-29358787 Msa0914650:CDS 5.0%
!! AAATTTATTAAAAAATACAA+AGG + chr6_2:29358245-29358264 Msa0914650:intron 5.0%
!! ATATATTGAAATATTATTTA+TGG + chr6_2:29358768-29358787 Msa0914650:CDS 5.0%
GAGACCAGATACCGTCTTCA+TGG - chr6_2:29358069-29358088 None:intergenic 50.0%
AAGACGGTATCTGGTCTCTC+AGG + chr6_2:29358071-29358090 Msa0914650:intron 50.0%
GAGACCAGATACCGTCTTCA+TGG - chr6_2:29358069-29358088 None:intergenic 50.0%
AAGACGGTATCTGGTCTCTC+AGG + chr6_2:29358071-29358090 Msa0914650:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 29357679 29358807 29357679 ID=Msa0914650;Name=Msa0914650
chr6_2 mRNA 29357679 29358807 29357679 ID=Msa0914650-mRNA-1;Parent=Msa0914650;Name=Msa0914650-mRNA-1;_AED=0.13;_eAED=0.23;_QI=51|0.5|0.33|1|0|0|3|0|122
chr6_2 exon 29357679 29357735 29357679 ID=Msa0914650-mRNA-1:exon:5310;Parent=Msa0914650-mRNA-1
chr6_2 exon 29358017 29358076 29358017 ID=Msa0914650-mRNA-1:exon:5311;Parent=Msa0914650-mRNA-1
chr6_2 exon 29358505 29358807 29358505 ID=Msa0914650-mRNA-1:exon:5312;Parent=Msa0914650-mRNA-1
chr6_2 five_prime_UTR 29357679 29357729 29357679 ID=Msa0914650-mRNA-1:five_prime_utr;Parent=Msa0914650-mRNA-1
chr6_2 CDS 29357730 29357735 29357730 ID=Msa0914650-mRNA-1:cds;Parent=Msa0914650-mRNA-1
chr6_2 CDS 29358017 29358076 29358017 ID=Msa0914650-mRNA-1:cds;Parent=Msa0914650-mRNA-1
chr6_2 CDS 29358505 29358807 29358505 ID=Msa0914650-mRNA-1:cds;Parent=Msa0914650-mRNA-1
Gene Sequence

>Msa0914650

ATGAAGAAACTAGTGTTAAAGGTAGATCTCTATGATGATAGAATCAAGAAAAAAGCCATGAAGACGGATAAGAAAATGACAATAATTGGAGACACAGATCCTGTTAATGTTGTGTCGAAGTTAAGGAAATTGTGTCATGCTGAAATAATTGCAATTGGACCAGCAAAAGAAGAGAAAAAAGAGGAAAGAAAGAAAGAAGAAGAAAAGAAAGAAGAAAAAAAGAAGGAGGAGCCAAAGAAAAAGGACACAAAAGATGAATTGGCTGATCTTATGAAGGCTTATGAAACATATTATAATCAAACGAAACAGCCATATCCTTATTTGTTAAGATATATTGAAATATTATTTATGGAATTTAAGAGTAGTTAA

Protein sequence

>Msa0914650

MKKLVLKVDLYDDRIKKKAMKTDKKMTIIGDTDPVNVVSKLRKLCHAEIIAIGPAKEEKKEERKKEEEKKEEKKKEEPKKKDTKDELADLMKAYETYYNQTKQPYPYLLRYIEILFMEFKSS*