AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0918400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCAGACGACTTCGATTT+TGG 0.115681 6_2:-46099175 Msa0918400:three_prime_UTR
CCAGTTGCTTTGCTGCTTCT+AGG 0.230202 6_2:-46099532 Msa0918400:three_prime_UTR
GTCTGCTTTCATGTTCAAAA+TGG 0.260239 6_2:-46099490 Msa0918400:three_prime_UTR
CAAAGATTAAAAGAATTATC+AGG 0.287216 6_2:+46099031 None:intergenic
TTGCTTGCTTCAGAAATATA+AGG 0.326840 6_2:+46099635 None:intergenic
TGCCTCATCTACCTGCTCTT+TGG 0.343328 6_2:-46099260 Msa0918400:three_prime_UTR
TGTAAGATAACATGTTCTTT+TGG 0.347950 6_2:-46099136 Msa0918400:three_prime_UTR
ACAAATAATCTGACAGTAAA+GGG 0.367788 6_2:+46099451 None:intergenic
TGTCAGATTATTTGTTTAGC+TGG 0.373302 6_2:-46099443 Msa0918400:three_prime_UTR
TGCTGCTTCTAGGGTTTCAA+TGG 0.376043 6_2:-46099522 Msa0918400:three_prime_UTR
AGCAGCAAAGCAACTGGCTT+TGG 0.376104 6_2:+46099538 None:intergenic
CAGTTGCTTTGCTGCTTCTA+GGG 0.401547 6_2:-46099531 Msa0918400:three_prime_UTR
AAGTTGTGCCTAATGGAGTT+GGG 0.432681 6_2:-46100776 None:intergenic
TTGTTTGTCAGCTTCTACCT+AGG 0.452066 6_2:+46098921 None:intergenic
CCTAGAAGCAGCAAAGCAAC+TGG 0.453522 6_2:+46099532 None:intergenic
ACAATGACATTAAAGACGTC+TGG 0.460611 6_2:+46099229 None:intergenic
AAACGCTTCACATCTAACTC+TGG 0.466143 6_2:+46099582 None:intergenic
CTTCGTACCTTTGAAGCCAT+TGG 0.470863 6_2:+46099072 None:intergenic
TTGAAACAAATGTTAAGTAA+TGG 0.474398 6_2:+46098949 None:intergenic
AACAAATAATCTGACAGTAA+AGG 0.475660 6_2:+46099450 None:intergenic
CATTTGTTTCAAAATTTCCT+AGG 0.482677 6_2:-46098938 Msa0918400:three_prime_UTR
CACGACATTCTGCATGATGA+TGG 0.483604 6_2:+46099368 None:intergenic
AAAGTTGTGCCTAATGGAGT+TGG 0.484051 6_2:-46100777 None:intergenic
TTATCAATTATTTGCCTCAA+GGG 0.487270 6_2:-46098848 Msa0918400:three_prime_UTR
ATATGAGGCATCCACAGATT+CGG 0.489287 6_2:-46100684 Msa0918400:CDS
CAACTGGCTTTGGCATGAGT+TGG 0.491247 6_2:+46099548 None:intergenic
ATAAATGCCAATGGCTTCAA+AGG 0.498141 6_2:-46099079 Msa0918400:three_prime_UTR
AAGAACAAGAGTTTGAATCA+AGG 0.508907 6_2:-46100748 Msa0918400:CDS
TTTATCAATTATTTGCCTCA+AGG 0.511450 6_2:-46098849 Msa0918400:three_prime_UTR
GAACTAAAATTGAAAGCCAT+GGG 0.512223 6_2:-46099696 Msa0918400:CDS
TTCTGTTAAATAAATGCCAA+TGG 0.514345 6_2:-46099088 Msa0918400:three_prime_UTR
AGAACAAGAGTTTGAATCAA+GGG 0.529400 6_2:-46100747 Msa0918400:CDS
TGTTTAGCTGGCTCATTCGT+AGG 0.529846 6_2:-46099431 Msa0918400:three_prime_UTR
GGAACTAAAATTGAAAGCCA+TGG 0.533259 6_2:-46099697 Msa0918400:CDS
GGAGCTTAGAAGTAAATATG+AGG 0.536877 6_2:-46100699 Msa0918400:CDS
AAATGTTAAGTAATGGAAGT+AGG 0.539480 6_2:+46098956 None:intergenic
AGTTGTGCCTAATGGAGTTG+GGG 0.555775 6_2:-46100775 None:intergenic
ATCCAAAGAGCAGGTAGATG+AGG 0.559257 6_2:+46099258 None:intergenic
AAAATCGAAGTCGTCTGCAA+TGG 0.561648 6_2:+46099177 None:intergenic
CATCCACAGATTCGGAAGCA+TGG 0.563427 6_2:-46100676 Msa0918400:intron
AACGCTTCACATCTAACTCT+GGG 0.565571 6_2:+46099583 None:intergenic
TGGAATAGCATCCAAAGAGC+AGG 0.568476 6_2:+46099249 None:intergenic
ATAGCAATATAACAGATAGC+AGG 0.571487 6_2:+46100413 None:intergenic
AAACCATGCTTCCGAATCTG+TGG 0.574654 6_2:+46100673 None:intergenic
GGCATATACTGCATTGTGCA+TGG 0.575854 6_2:+46099279 None:intergenic
AAAGATTAAAAGAATTATCA+GGG 0.579114 6_2:+46099032 None:intergenic
TTAGCTATCAAATGTTAACT+CGG 0.593697 6_2:+46099394 None:intergenic
ATTACATAAAAGTCATCTCG+AGG 0.598565 6_2:+46098888 None:intergenic
ACAGATATCAGCAGCCCTTG+AGG 0.603275 6_2:+46098834 None:intergenic
ACAAGATAATATTGATGTGG+TGG 0.622268 6_2:-46100720 Msa0918400:CDS
GTTGTGCCTAATGGAGTTGG+GGG 0.637160 6_2:-46100774 None:intergenic
ACTAAAATTGAAAGCCATGG+GGG 0.646756 6_2:-46099694 Msa0918400:CDS
TTACATAAAAGTCATCTCGA+GGG 0.652774 6_2:+46098889 None:intergenic
AGGAGATTATCCAATAAACC+AGG 0.654576 6_2:+46099655 None:intergenic
TGAACAAGATAATATTGATG+TGG 0.663705 6_2:-46100723 Msa0918400:CDS
CAATATAACAGATAGCAGGA+TGG 0.674476 6_2:+46100417 None:intergenic
ATGGCTTCAAAGGTACGAAG+AGG 0.680485 6_2:-46099069 Msa0918400:three_prime_UTR
AACTAAAATTGAAAGCCATG+GGG 0.687621 6_2:-46099695 Msa0918400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATGTTTTTATTTTTATT+AGG + chr6_2:46099847-46099866 None:intergenic 10.0%
!!! TCAATGTTTTTATTTTTATT+AGG + chr6_2:46099847-46099866 None:intergenic 10.0%
!!! ACAATTAAAACTAATTTTGA+TGG - chr6_2:46099036-46099055 Msa0918400:three_prime_UTR 15.0%
!! TTATAGTTATTAAGTATGAT+TGG - chr6_2:46099075-46099094 Msa0918400:three_prime_UTR 15.0%
!! AAAGATTAAAAGAATTATCA+GGG + chr6_2:46100582-46100601 None:intergenic 15.0%
!!! ACAATTAAAACTAATTTTGA+TGG - chr6_2:46099036-46099055 Msa0918400:three_prime_UTR 15.0%
!! TTATAGTTATTAAGTATGAT+TGG - chr6_2:46099075-46099094 Msa0918400:three_prime_UTR 15.0%
!! AAAGATTAAAAGAATTATCA+GGG + chr6_2:46100582-46100601 None:intergenic 15.0%
!!! CTTATAGAACTTCATTTTAA+TGG - chr6_2:46098967-46098986 Msa0918400:three_prime_UTR 20.0%
!! AGAAAGATACACAAAATTAA+AGG + chr6_2:46099102-46099121 None:intergenic 20.0%
!! TGATTATTTATGTATCTTGT+TGG - chr6_2:46099124-46099143 Msa0918400:three_prime_UTR 20.0%
!! ATAGATTGAATAAAATGTGT+TGG + chr6_2:46099482-46099501 None:intergenic 20.0%
!! GTAACACATTAAGAAAAAAA+AGG + chr6_2:46099533-46099552 None:intergenic 20.0%
!! ATTGTTTGATCAAGATAATT+TGG + chr6_2:46099769-46099788 None:intergenic 20.0%
!! CAAAGATTAAAAGAATTATC+AGG + chr6_2:46100583-46100602 None:intergenic 20.0%
!! TTGAAACAAATGTTAAGTAA+TGG + chr6_2:46100665-46100684 None:intergenic 20.0%
!!! CTTATAGAACTTCATTTTAA+TGG - chr6_2:46098967-46098986 Msa0918400:three_prime_UTR 20.0%
!! AGAAAGATACACAAAATTAA+AGG + chr6_2:46099102-46099121 None:intergenic 20.0%
!! TGATTATTTATGTATCTTGT+TGG - chr6_2:46099124-46099143 Msa0918400:three_prime_UTR 20.0%
!! ATAGATTGAATAAAATGTGT+TGG + chr6_2:46099482-46099501 None:intergenic 20.0%
!! GTAACACATTAAGAAAAAAA+AGG + chr6_2:46099533-46099552 None:intergenic 20.0%
!! ATTGTTTGATCAAGATAATT+TGG + chr6_2:46099769-46099788 None:intergenic 20.0%
!! CAAAGATTAAAAGAATTATC+AGG + chr6_2:46100583-46100602 None:intergenic 20.0%
!! TTGAAACAAATGTTAAGTAA+TGG + chr6_2:46100665-46100684 None:intergenic 20.0%
! TGAACAAGATAATATTGATG+TGG - chr6_2:46098888-46098907 Msa0918400:three_prime_UTR 25.0%
! TTAAGAAAAAAAAGGCTAAC+AGG + chr6_2:46099525-46099544 None:intergenic 25.0%
! ACAATCATAACAAGCAATAT+TGG + chr6_2:46099555-46099574 None:intergenic 25.0%
!! TAGTTTGATAACAAGGAAAA+TGG - chr6_2:46099619-46099638 Msa0918400:exon 25.0%
! TTATCTTGATCAAACAATCA+CGG - chr6_2:46099771-46099790 Msa0918400:intron 25.0%
! ACAAATAATCTGACAGTAAA+GGG + chr6_2:46100163-46100182 None:intergenic 25.0%
! AACAAATAATCTGACAGTAA+AGG + chr6_2:46100164-46100183 None:intergenic 25.0%
! TTAGCTATCAAATGTTAACT+CGG + chr6_2:46100220-46100239 None:intergenic 25.0%
!! TGTAAGATAACATGTTCTTT+TGG - chr6_2:46100475-46100494 Msa0918400:intron 25.0%
! TTCTGTTAAATAAATGCCAA+TGG - chr6_2:46100523-46100542 Msa0918400:intron 25.0%
!! AAATGTTAAGTAATGGAAGT+AGG + chr6_2:46100658-46100677 None:intergenic 25.0%
! CATTTGTTTCAAAATTTCCT+AGG - chr6_2:46100673-46100692 Msa0918400:intron 25.0%
! TTTATCAATTATTTGCCTCA+AGG - chr6_2:46100762-46100781 Msa0918400:CDS 25.0%
! TTATCAATTATTTGCCTCAA+GGG - chr6_2:46100763-46100782 Msa0918400:CDS 25.0%
! TGAACAAGATAATATTGATG+TGG - chr6_2:46098888-46098907 Msa0918400:three_prime_UTR 25.0%
! TTAAGAAAAAAAAGGCTAAC+AGG + chr6_2:46099525-46099544 None:intergenic 25.0%
! ACAATCATAACAAGCAATAT+TGG + chr6_2:46099555-46099574 None:intergenic 25.0%
!! TAGTTTGATAACAAGGAAAA+TGG - chr6_2:46099619-46099638 Msa0918400:exon 25.0%
! TTATCTTGATCAAACAATCA+CGG - chr6_2:46099771-46099790 Msa0918400:intron 25.0%
! ACAAATAATCTGACAGTAAA+GGG + chr6_2:46100163-46100182 None:intergenic 25.0%
! AACAAATAATCTGACAGTAA+AGG + chr6_2:46100164-46100183 None:intergenic 25.0%
! TTAGCTATCAAATGTTAACT+CGG + chr6_2:46100220-46100239 None:intergenic 25.0%
!! TGTAAGATAACATGTTCTTT+TGG - chr6_2:46100475-46100494 Msa0918400:intron 25.0%
! TTCTGTTAAATAAATGCCAA+TGG - chr6_2:46100523-46100542 Msa0918400:intron 25.0%
!! AAATGTTAAGTAATGGAAGT+AGG + chr6_2:46100658-46100677 None:intergenic 25.0%
! CATTTGTTTCAAAATTTCCT+AGG - chr6_2:46100673-46100692 Msa0918400:intron 25.0%
! TTTATCAATTATTTGCCTCA+AGG - chr6_2:46100762-46100781 Msa0918400:CDS 25.0%
! TTATCAATTATTTGCCTCAA+GGG - chr6_2:46100763-46100782 Msa0918400:CDS 25.0%
AAGAACAAGAGTTTGAATCA+AGG - chr6_2:46098863-46098882 Msa0918400:three_prime_UTR 30.0%
AGAACAAGAGTTTGAATCAA+GGG - chr6_2:46098864-46098883 Msa0918400:three_prime_UTR 30.0%
ACAAGATAATATTGATGTGG+TGG - chr6_2:46098891-46098910 Msa0918400:three_prime_UTR 30.0%
TTGATGGAAAATGACAAAGT+TGG - chr6_2:46099052-46099071 Msa0918400:three_prime_UTR 30.0%
ATAGCAATATAACAGATAGC+AGG + chr6_2:46099201-46099220 None:intergenic 30.0%
!!! GCTATTGCATTTTTGAAGTT+CGG - chr6_2:46099216-46099235 Msa0918400:three_prime_UTR 30.0%
! TATGTTGTTTATCCCAGATA+CGG + chr6_2:46099251-46099270 None:intergenic 30.0%
AGACTTTATATGTGTATGCA+TGG - chr6_2:46099351-46099370 Msa0918400:three_prime_UTR 30.0%
GAACTAAAATTGAAAGCCAT+GGG - chr6_2:46099915-46099934 Msa0918400:intron 30.0%
AACTAAAATTGAAAGCCATG+GGG - chr6_2:46099916-46099935 Msa0918400:intron 30.0%
TTGCTTGCTTCAGAAATATA+AGG + chr6_2:46099979-46099998 None:intergenic 30.0%
TGTCAGATTATTTGTTTAGC+TGG - chr6_2:46100168-46100187 Msa0918400:intron 30.0%
TTACATAAAAGTCATCTCGA+GGG + chr6_2:46100725-46100744 None:intergenic 30.0%
ATTACATAAAAGTCATCTCG+AGG + chr6_2:46100726-46100745 None:intergenic 30.0%
AAGAACAAGAGTTTGAATCA+AGG - chr6_2:46098863-46098882 Msa0918400:three_prime_UTR 30.0%
AGAACAAGAGTTTGAATCAA+GGG - chr6_2:46098864-46098883 Msa0918400:three_prime_UTR 30.0%
ACAAGATAATATTGATGTGG+TGG - chr6_2:46098891-46098910 Msa0918400:three_prime_UTR 30.0%
TTGATGGAAAATGACAAAGT+TGG - chr6_2:46099052-46099071 Msa0918400:three_prime_UTR 30.0%
ATAGCAATATAACAGATAGC+AGG + chr6_2:46099201-46099220 None:intergenic 30.0%
!!! GCTATTGCATTTTTGAAGTT+CGG - chr6_2:46099216-46099235 Msa0918400:three_prime_UTR 30.0%
! TATGTTGTTTATCCCAGATA+CGG + chr6_2:46099251-46099270 None:intergenic 30.0%
AGACTTTATATGTGTATGCA+TGG - chr6_2:46099351-46099370 Msa0918400:three_prime_UTR 30.0%
GAACTAAAATTGAAAGCCAT+GGG - chr6_2:46099915-46099934 Msa0918400:intron 30.0%
AACTAAAATTGAAAGCCATG+GGG - chr6_2:46099916-46099935 Msa0918400:intron 30.0%
TTGCTTGCTTCAGAAATATA+AGG + chr6_2:46099979-46099998 None:intergenic 30.0%
TGTCAGATTATTTGTTTAGC+TGG - chr6_2:46100168-46100187 Msa0918400:intron 30.0%
TTACATAAAAGTCATCTCGA+GGG + chr6_2:46100725-46100744 None:intergenic 30.0%
ATTACATAAAAGTCATCTCG+AGG + chr6_2:46100726-46100745 None:intergenic 30.0%
GGAGCTTAGAAGTAAATATG+AGG - chr6_2:46098912-46098931 Msa0918400:three_prime_UTR 35.0%
CAATATAACAGATAGCAGGA+TGG + chr6_2:46099197-46099216 None:intergenic 35.0%
TTTCCTTAGAATCCGACATA+AGG - chr6_2:46099392-46099411 Msa0918400:three_prime_UTR 35.0%
TTCCTTAGAATCCGACATAA+GGG - chr6_2:46099393-46099412 Msa0918400:three_prime_UTR 35.0%
! CTGAGTCTAGTTTGATAACA+AGG - chr6_2:46099612-46099631 Msa0918400:exon 35.0%
AACATCTGCAAGCAAAAATG+TGG + chr6_2:46099894-46099913 None:intergenic 35.0%
! ACATTTTTGCTTGCAGATGT+TGG - chr6_2:46099893-46099912 Msa0918400:intron 35.0%
GGAACTAAAATTGAAAGCCA+TGG - chr6_2:46099914-46099933 Msa0918400:intron 35.0%
ACTAAAATTGAAAGCCATGG+GGG - chr6_2:46099917-46099936 Msa0918400:intron 35.0%
AGGAGATTATCCAATAAACC+AGG + chr6_2:46099959-46099978 None:intergenic 35.0%
GTCTGCTTTCATGTTCAAAA+TGG - chr6_2:46100121-46100140 Msa0918400:intron 35.0%
ACAATGACATTAAAGACGTC+TGG + chr6_2:46100385-46100404 None:intergenic 35.0%
ATAAATGCCAATGGCTTCAA+AGG - chr6_2:46100532-46100551 Msa0918400:intron 35.0%
GGAGCTTAGAAGTAAATATG+AGG - chr6_2:46098912-46098931 Msa0918400:three_prime_UTR 35.0%
CAATATAACAGATAGCAGGA+TGG + chr6_2:46099197-46099216 None:intergenic 35.0%
TTTCCTTAGAATCCGACATA+AGG - chr6_2:46099392-46099411 Msa0918400:three_prime_UTR 35.0%
TTCCTTAGAATCCGACATAA+GGG - chr6_2:46099393-46099412 Msa0918400:three_prime_UTR 35.0%
! CTGAGTCTAGTTTGATAACA+AGG - chr6_2:46099612-46099631 Msa0918400:exon 35.0%
AACATCTGCAAGCAAAAATG+TGG + chr6_2:46099894-46099913 None:intergenic 35.0%
! ACATTTTTGCTTGCAGATGT+TGG - chr6_2:46099893-46099912 Msa0918400:intron 35.0%
GGAACTAAAATTGAAAGCCA+TGG - chr6_2:46099914-46099933 Msa0918400:intron 35.0%
ACTAAAATTGAAAGCCATGG+GGG - chr6_2:46099917-46099936 Msa0918400:intron 35.0%
AGGAGATTATCCAATAAACC+AGG + chr6_2:46099959-46099978 None:intergenic 35.0%
GTCTGCTTTCATGTTCAAAA+TGG - chr6_2:46100121-46100140 Msa0918400:intron 35.0%
ACAATGACATTAAAGACGTC+TGG + chr6_2:46100385-46100404 None:intergenic 35.0%
ATAAATGCCAATGGCTTCAA+AGG - chr6_2:46100532-46100551 Msa0918400:intron 35.0%
! ATATGAGGCATCCACAGATT+CGG - chr6_2:46098927-46098946 Msa0918400:three_prime_UTR 40.0%
ATCCCTTATGTCGGATTCTA+AGG + chr6_2:46099398-46099417 None:intergenic 40.0%
TAGTGGTACATCCCTTATGT+CGG + chr6_2:46099407-46099426 None:intergenic 40.0%
! TAGTAGAGAGTGTACTGTAG+CGG + chr6_2:46099444-46099463 None:intergenic 40.0%
! AACAAGGAAAATGGATTCCG+AGG - chr6_2:46099628-46099647 Msa0918400:exon 40.0%
! TATTGACTGCGTGAATGAAC+GGG + chr6_2:46099671-46099690 None:intergenic 40.0%
!! GTATTGACTGCGTGAATGAA+CGG + chr6_2:46099672-46099691 None:intergenic 40.0%
CATTCACGCAGTCAATACAT+TGG - chr6_2:46099674-46099693 Msa0918400:CDS 40.0%
!! ATGTGCTGACTGTGTGAATT+TGG - chr6_2:46099794-46099813 Msa0918400:intron 40.0%
! TGTGTGAATTTGGAGTATCC+CGG - chr6_2:46099804-46099823 Msa0918400:intron 40.0%
!! GTTTTGGTCACCTGGTTTAT+TGG - chr6_2:46099946-46099965 Msa0918400:intron 40.0%
AACGCTTCACATCTAACTCT+GGG + chr6_2:46100031-46100050 None:intergenic 40.0%
AAACGCTTCACATCTAACTC+TGG + chr6_2:46100032-46100051 None:intergenic 40.0%
AAAATCGAAGTCGTCTGCAA+TGG + chr6_2:46100437-46100456 None:intergenic 40.0%
! ATTGCAGACGACTTCGATTT+TGG - chr6_2:46100436-46100455 Msa0918400:CDS 40.0%
! TTGCAGACGACTTCGATTTT+GGG - chr6_2:46100437-46100456 Msa0918400:CDS 40.0%
! CAATGCTTTTCACGTATCTG+AGG + chr6_2:46100501-46100520 None:intergenic 40.0%
TTGTTTGTCAGCTTCTACCT+AGG + chr6_2:46100693-46100712 None:intergenic 40.0%
! ATATGAGGCATCCACAGATT+CGG - chr6_2:46098927-46098946 Msa0918400:three_prime_UTR 40.0%
ATCCCTTATGTCGGATTCTA+AGG + chr6_2:46099398-46099417 None:intergenic 40.0%
TAGTGGTACATCCCTTATGT+CGG + chr6_2:46099407-46099426 None:intergenic 40.0%
! TAGTAGAGAGTGTACTGTAG+CGG + chr6_2:46099444-46099463 None:intergenic 40.0%
! AACAAGGAAAATGGATTCCG+AGG - chr6_2:46099628-46099647 Msa0918400:exon 40.0%
! TATTGACTGCGTGAATGAAC+GGG + chr6_2:46099671-46099690 None:intergenic 40.0%
!! GTATTGACTGCGTGAATGAA+CGG + chr6_2:46099672-46099691 None:intergenic 40.0%
CATTCACGCAGTCAATACAT+TGG - chr6_2:46099674-46099693 Msa0918400:CDS 40.0%
!! ATGTGCTGACTGTGTGAATT+TGG - chr6_2:46099794-46099813 Msa0918400:intron 40.0%
! TGTGTGAATTTGGAGTATCC+CGG - chr6_2:46099804-46099823 Msa0918400:intron 40.0%
!! GTTTTGGTCACCTGGTTTAT+TGG - chr6_2:46099946-46099965 Msa0918400:intron 40.0%
AACGCTTCACATCTAACTCT+GGG + chr6_2:46100031-46100050 None:intergenic 40.0%
AAACGCTTCACATCTAACTC+TGG + chr6_2:46100032-46100051 None:intergenic 40.0%
AAAATCGAAGTCGTCTGCAA+TGG + chr6_2:46100437-46100456 None:intergenic 40.0%
! ATTGCAGACGACTTCGATTT+TGG - chr6_2:46100436-46100455 Msa0918400:CDS 40.0%
! TTGCAGACGACTTCGATTTT+GGG - chr6_2:46100437-46100456 Msa0918400:CDS 40.0%
! CAATGCTTTTCACGTATCTG+AGG + chr6_2:46100501-46100520 None:intergenic 40.0%
TTGTTTGTCAGCTTCTACCT+AGG + chr6_2:46100693-46100712 None:intergenic 40.0%
AAACCATGCTTCCGAATCTG+TGG + chr6_2:46098941-46098960 None:intergenic 45.0%
TCACGCAGTCAATACATTGG+TGG - chr6_2:46099677-46099696 Msa0918400:CDS 45.0%
! CAGTTGCTTTGCTGCTTCTA+GGG - chr6_2:46100080-46100099 Msa0918400:intron 45.0%
!! TGCTGCTTCTAGGGTTTCAA+TGG - chr6_2:46100089-46100108 Msa0918400:intron 45.0%
! TGTTTAGCTGGCTCATTCGT+AGG - chr6_2:46100180-46100199 Msa0918400:intron 45.0%
CACGACATTCTGCATGATGA+TGG + chr6_2:46100246-46100265 None:intergenic 45.0%
GGCATATACTGCATTGTGCA+TGG + chr6_2:46100335-46100354 None:intergenic 45.0%
ATCCAAAGAGCAGGTAGATG+AGG + chr6_2:46100356-46100375 None:intergenic 45.0%
TGGAATAGCATCCAAAGAGC+AGG + chr6_2:46100365-46100384 None:intergenic 45.0%
!! CTTCGTACCTTTGAAGCCAT+TGG + chr6_2:46100542-46100561 None:intergenic 45.0%
! ATGGCTTCAAAGGTACGAAG+AGG - chr6_2:46100542-46100561 Msa0918400:intron 45.0%
AAACCATGCTTCCGAATCTG+TGG + chr6_2:46098941-46098960 None:intergenic 45.0%
TCACGCAGTCAATACATTGG+TGG - chr6_2:46099677-46099696 Msa0918400:CDS 45.0%
! CAGTTGCTTTGCTGCTTCTA+GGG - chr6_2:46100080-46100099 Msa0918400:intron 45.0%
!! TGCTGCTTCTAGGGTTTCAA+TGG - chr6_2:46100089-46100108 Msa0918400:intron 45.0%
! TGTTTAGCTGGCTCATTCGT+AGG - chr6_2:46100180-46100199 Msa0918400:intron 45.0%
CACGACATTCTGCATGATGA+TGG + chr6_2:46100246-46100265 None:intergenic 45.0%
GGCATATACTGCATTGTGCA+TGG + chr6_2:46100335-46100354 None:intergenic 45.0%
ATCCAAAGAGCAGGTAGATG+AGG + chr6_2:46100356-46100375 None:intergenic 45.0%
TGGAATAGCATCCAAAGAGC+AGG + chr6_2:46100365-46100384 None:intergenic 45.0%
!! CTTCGTACCTTTGAAGCCAT+TGG + chr6_2:46100542-46100561 None:intergenic 45.0%
! ATGGCTTCAAAGGTACGAAG+AGG - chr6_2:46100542-46100561 Msa0918400:intron 45.0%
CATCCACAGATTCGGAAGCA+TGG - chr6_2:46098935-46098954 Msa0918400:three_prime_UTR 50.0%
GATGTACCACTACCCCACTA+TGG - chr6_2:46099415-46099434 Msa0918400:three_prime_UTR 50.0%
!! ACTGTAGCGGACACCATAGT+GGG + chr6_2:46099431-46099450 None:intergenic 50.0%
!! TACTGTAGCGGACACCATAG+TGG + chr6_2:46099432-46099451 None:intergenic 50.0%
ATTCCGAGGTCCCTGTAGTT+GGG - chr6_2:46099642-46099661 Msa0918400:CDS 50.0%
TATCCCAACTACAGGGACCT+CGG + chr6_2:46099648-46099667 None:intergenic 50.0%
GAACGGGTATCCCAACTACA+GGG + chr6_2:46099655-46099674 None:intergenic 50.0%
TGAACGGGTATCCCAACTAC+AGG + chr6_2:46099656-46099675 None:intergenic 50.0%
! ATTTGGAGTATCCCGGTTGC+AGG - chr6_2:46099811-46099830 Msa0918400:intron 50.0%
! TTTGGAGTATCCCGGTTGCA+GGG - chr6_2:46099812-46099831 Msa0918400:intron 50.0%
ACCAAAACTCATTGCCCCCA+TGG + chr6_2:46099934-46099953 None:intergenic 50.0%
!! GGCAATGAGTTTTGGTCACC+TGG - chr6_2:46099938-46099957 Msa0918400:intron 50.0%
!! CAACTGGCTTTGGCATGAGT+TGG + chr6_2:46100066-46100085 None:intergenic 50.0%
! AGCAGCAAAGCAACTGGCTT+TGG + chr6_2:46100076-46100095 None:intergenic 50.0%
CCTAGAAGCAGCAAAGCAAC+TGG + chr6_2:46100082-46100101 None:intergenic 50.0%
! CCAGTTGCTTTGCTGCTTCT+AGG - chr6_2:46100079-46100098 Msa0918400:intron 50.0%
TGCCTCATCTACCTGCTCTT+TGG - chr6_2:46100351-46100370 Msa0918400:intron 50.0%
CATCCACAGATTCGGAAGCA+TGG - chr6_2:46098935-46098954 Msa0918400:three_prime_UTR 50.0%
GATGTACCACTACCCCACTA+TGG - chr6_2:46099415-46099434 Msa0918400:three_prime_UTR 50.0%
!! ACTGTAGCGGACACCATAGT+GGG + chr6_2:46099431-46099450 None:intergenic 50.0%
!! TACTGTAGCGGACACCATAG+TGG + chr6_2:46099432-46099451 None:intergenic 50.0%
ATTCCGAGGTCCCTGTAGTT+GGG - chr6_2:46099642-46099661 Msa0918400:CDS 50.0%
TATCCCAACTACAGGGACCT+CGG + chr6_2:46099648-46099667 None:intergenic 50.0%
GAACGGGTATCCCAACTACA+GGG + chr6_2:46099655-46099674 None:intergenic 50.0%
TGAACGGGTATCCCAACTAC+AGG + chr6_2:46099656-46099675 None:intergenic 50.0%
! ATTTGGAGTATCCCGGTTGC+AGG - chr6_2:46099811-46099830 Msa0918400:intron 50.0%
! TTTGGAGTATCCCGGTTGCA+GGG - chr6_2:46099812-46099831 Msa0918400:intron 50.0%
ACCAAAACTCATTGCCCCCA+TGG + chr6_2:46099934-46099953 None:intergenic 50.0%
!! GGCAATGAGTTTTGGTCACC+TGG - chr6_2:46099938-46099957 Msa0918400:intron 50.0%
!! CAACTGGCTTTGGCATGAGT+TGG + chr6_2:46100066-46100085 None:intergenic 50.0%
! AGCAGCAAAGCAACTGGCTT+TGG + chr6_2:46100076-46100095 None:intergenic 50.0%
CCTAGAAGCAGCAAAGCAAC+TGG + chr6_2:46100082-46100101 None:intergenic 50.0%
! CCAGTTGCTTTGCTGCTTCT+AGG - chr6_2:46100079-46100098 Msa0918400:intron 50.0%
TGCCTCATCTACCTGCTCTT+TGG - chr6_2:46100351-46100370 Msa0918400:intron 50.0%
!! CTGTAGCGGACACCATAGTG+GGG + chr6_2:46099430-46099449 None:intergenic 55.0%
GATTCCGAGGTCCCTGTAGT+TGG - chr6_2:46099641-46099660 Msa0918400:CDS 55.0%
! TTGGAGTATCCCGGTTGCAG+GGG - chr6_2:46099813-46099832 Msa0918400:intron 55.0%
GATCAAGATCCCCTGCAACC+GGG + chr6_2:46099825-46099844 None:intergenic 55.0%
GGATCAAGATCCCCTGCAAC+CGG + chr6_2:46099826-46099845 None:intergenic 55.0%
! GCCATGGGGGCAATGAGTTT+TGG - chr6_2:46099930-46099949 Msa0918400:intron 55.0%
!! CTGTAGCGGACACCATAGTG+GGG + chr6_2:46099430-46099449 None:intergenic 55.0%
GATTCCGAGGTCCCTGTAGT+TGG - chr6_2:46099641-46099660 Msa0918400:CDS 55.0%
! TTGGAGTATCCCGGTTGCAG+GGG - chr6_2:46099813-46099832 Msa0918400:intron 55.0%
GATCAAGATCCCCTGCAACC+GGG + chr6_2:46099825-46099844 None:intergenic 55.0%
GGATCAAGATCCCCTGCAAC+CGG + chr6_2:46099826-46099845 None:intergenic 55.0%
! GCCATGGGGGCAATGAGTTT+TGG - chr6_2:46099930-46099949 Msa0918400:intron 55.0%
! CGGACACCATAGTGGGGTAG+TGG + chr6_2:46099424-46099443 None:intergenic 60.0%
! CGGACACCATAGTGGGGTAG+TGG + chr6_2:46099424-46099443 None:intergenic 60.0%
TCGGCGCTATGCGCCGTATC+TGG - chr6_2:46099235-46099254 Msa0918400:three_prime_UTR 65.0%
CGGCGCTATGCGCCGTATCT+GGG - chr6_2:46099236-46099255 Msa0918400:three_prime_UTR 65.0%
TCGGCGCTATGCGCCGTATC+TGG - chr6_2:46099235-46099254 Msa0918400:three_prime_UTR 65.0%
CGGCGCTATGCGCCGTATCT+GGG - chr6_2:46099236-46099255 Msa0918400:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 46098847 46100786 46098847 ID=Msa0918400;Name=Msa0918400
chr6_2 mRNA 46098847 46100786 46098847 ID=Msa0918400-mRNA-1;Parent=Msa0918400;Name=Msa0918400-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|0|0.33|0.33|1|1|3|783|92
chr6_2 exon 46098847 46099724 46098847 ID=Msa0918400-mRNA-1:exon:7218;Parent=Msa0918400-mRNA-1
chr6_2 exon 46100384 46100457 46100384 ID=Msa0918400-mRNA-1:exon:7217;Parent=Msa0918400-mRNA-1
chr6_2 exon 46100677 46100786 46100677 ID=Msa0918400-mRNA-1:exon:7216;Parent=Msa0918400-mRNA-1
chr6_2 CDS 46100677 46100786 46100677 ID=Msa0918400-mRNA-1:cds;Parent=Msa0918400-mRNA-1
chr6_2 CDS 46100384 46100457 46100384 ID=Msa0918400-mRNA-1:cds;Parent=Msa0918400-mRNA-1
chr6_2 CDS 46099630 46099724 46099630 ID=Msa0918400-mRNA-1:cds;Parent=Msa0918400-mRNA-1
chr6_2 three_prime_UTR 46098847 46099629 46098847 ID=Msa0918400-mRNA-1:three_prime_utr;Parent=Msa0918400-mRNA-1
Gene Sequence

>Msa0918400

ATGGAGTTGGGGGTTGAAGAACAAGAGTTTGAATCAAGGGATGAACAAGATAATATTGATGTGGTGGAGCTTAGAAGTAAATATGAGGCATCCACAGATTCGGAAGCATGTAGTCTTCACAATAGCTGCCATCCTGCTATCTGTTATATTGCTATTGCATTTTTGAAGTTCGGCGCTATGCGCCATGTTGGAACTAAAATTGAAAGCCATGGGGGCAATGAGTTTTGGTCACCTGGTTTATTGGATAATCTCCTTATATTTCTGAAGCAAGCAATGTGA
Protein sequence

>Msa0918400

MELGVEEQEFESRDEQDNIDVVELRSKYEASTDSEACSLHNSCHPAICYIAIAFLKFGAMRHVGTKIESHGGNEFWSPGLLDNLLIFLKQAM*